CN108699515A - 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 - Google Patents

由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 Download PDF

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CN108699515A
CN108699515A CN201780013255.3A CN201780013255A CN108699515A CN 108699515 A CN108699515 A CN 108699515A CN 201780013255 A CN201780013255 A CN 201780013255A CN 108699515 A CN108699515 A CN 108699515A
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cells
pluripotent stem
derived
endocrine progenitor
human pluripotent
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N.S.克里斯特弗森
U.多恩
M.汉森
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Novo Nordisk AS
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/845—Gamma amino butyric acid [GABA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201780013255.3A 2016-02-24 2017-02-24 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 Pending CN108699515A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16157181.5 2016-02-24
EP16157181 2016-02-24
PCT/EP2017/054390 WO2017144695A1 (en) 2016-02-24 2017-02-24 Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors

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CN108699515A true CN108699515A (zh) 2018-10-23

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CN201780013255.3A Pending CN108699515A (zh) 2016-02-24 2017-02-24 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞

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US (1) US11274280B2 (https=)
EP (1) EP3420073B1 (https=)
JP (1) JP7123802B2 (https=)
CN (1) CN108699515A (https=)
ES (1) ES2967942T3 (https=)
MA (1) MA45747A (https=)
WO (1) WO2017144695A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113069440A (zh) * 2021-04-08 2021-07-06 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基
CN116286663A (zh) * 2016-06-21 2023-06-23 詹森生物科技公司 人多能干细胞衍生的功能性β细胞的体外产生

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KR102203056B1 (ko) 2011-12-22 2021-01-14 얀센 바이오테크 인코포레이티드 인간 배아 줄기 세포의 단일 인슐린 호르몬 양성 세포로의 분화
SG11201510177WA (en) 2013-06-11 2016-01-28 Harvard College SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
US10006006B2 (en) 2014-05-16 2018-06-26 Janssen Biotech, Inc. Use of small molecules to enhance MAFA expression in pancreatic endocrine cells
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
WO2017144695A1 (en) 2016-02-24 2017-08-31 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
AU2018330499B2 (en) * 2017-09-11 2025-01-30 Novo Nordisk A/S Enrichment of NKX6.1 and C-peptide co-expressing cells derived in vitro from stem cells
KR20250048146A (ko) 2017-11-15 2025-04-07 셈마 테라퓨틱스, 인크. 섬세포 제조 조성물 및 사용 방법
CN112074281A (zh) * 2018-03-02 2020-12-11 沃泰克斯药物股份有限公司 增强干细胞向β细胞分化的方法
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
AU2018439389A1 (en) * 2018-08-30 2021-02-18 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
CN113728090B (zh) 2019-04-08 2024-04-05 诺和诺德股份有限公司 从干细胞衍生的定形内胚层生成胰腺内胚层
TW202115245A (zh) 2019-06-27 2021-04-16 丹麥商諾佛 儂迪克股份有限公司 安全免疫隱形細胞
CN114729323B (zh) 2019-11-22 2024-09-17 诺和诺德股份有限公司 旋转聚集的神经微球及其应用
WO2021174201A1 (en) * 2020-02-28 2021-09-02 The Trustees Of The University Of Pennsylvania Tissue culture media for survival and engraftment of human pancreatic islets
JP2023516672A (ja) 2020-03-02 2023-04-20 ノヴォ ノルディスク アー／エス 汚染性残留未分化多能性幹細胞を検出するための多能性マーカーの使用
CN116018150A (zh) 2020-08-28 2023-04-25 诺和诺德股份有限公司 用于筛选干细胞衍生的β样细胞的体外群体的方法及其新型标志物
WO2022129472A1 (en) 2020-12-18 2022-06-23 Novo Nordisk A/S Safe immuno-stealth cells
WO2022136215A1 (en) 2020-12-21 2022-06-30 Novo Nordisk A/S Safe immuno-stealth cells
WO2023076554A1 (en) * 2021-10-28 2023-05-04 Memorial Sloan-Kettering Cancer Center Compositions and methods for promoting in vitro maturation of cells
WO2023118050A1 (en) 2021-12-21 2023-06-29 Novo Nordisk A/S Use of novel markers to detect pluripotent stem cells
WO2024151541A1 (en) 2023-01-09 2024-07-18 Sana Biotechnology, Inc. Type-1 diabetes autoimmune mouse
CN121604968A (zh) 2023-05-22 2026-03-03 萨那生物科技公司 递送胰岛细胞的方法及相关方法
WO2025093467A1 (en) 2023-10-31 2025-05-08 Novo Nordisk A/S Methods for preventing unwanted cells arising during differentiation of human pluripotent stem cells

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WO2014201167A1 (en) * 2013-06-11 2014-12-18 President And Fellows Of Harvard College SC-β CELLS AND COMPOSITIONS AND METHODS FOR GENERATING THE SAME
WO2015028614A1 (en) * 2013-08-30 2015-03-05 Novo Nordisk A/S Generation of endocrine progenitor cells from human pluripotent stem cells using small molecules
CN104411815A (zh) * 2012-04-30 2015-03-11 大学健康网络 用于从hPSC生成胰腺祖细胞和功能性β细胞的方法和组合物
CN104903440A (zh) * 2012-09-03 2015-09-09 诺和诺德股份有限公司 使用小分子从多能干细胞产生胰内胚层
US20150250824A1 (en) * 2014-03-07 2015-09-10 The Research Foundation For The State University Of New York Methods and compositions for expansion of stem cells and other cells

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WO2012175633A1 (en) 2011-06-21 2012-12-27 Novo Nordisk A/S Efficient induction of definitive endoderm from pluripotent stem cells
EP2900820B2 (en) * 2012-09-27 2020-11-18 Institut National de la Sante et de la Recherche Medicale (INSERM) Methods for producing a population of pancreatic beta-cells
JP6557147B2 (ja) 2012-12-31 2019-08-07 ヤンセン バイオテツク，インコーポレーテツド Ｈｂ９調節物を用いたヒト胚性幹細胞の膵臓内分泌細胞への分化
US20150368616A1 (en) * 2013-02-14 2015-12-24 The Cleveland Clinic Foundation Methods for induction of cell fates from pluripotent cells
JP2016521971A (ja) 2013-04-23 2016-07-28 イェダ リサーチ アンド ディベロップメント カンパニー リミテッド 単離ナイーブ型多能性幹細胞およびそれを発生させる方法関連出願本出願は、米国特許法１１９条第（ｅ）項に基づき、２０１４年１月２９日出願の米国特許仮出願第６１／９３２，９３５号、２０１３年９月１７日出願の米国特許仮出願第６１／８７８，７６９号、および２０１３年４月２３日出願の米国特許仮出願第６１／８１４，９２０号の優先権を主張する。また、本出願は、同時に提出された同出願人による同時係属出願である、ＹａｑｕｂＨＡＮＮＡ、ＮｏａＮＯＶＥＲＳＨＴＥＲＮ、およびＹｏａｃｈＲＡＩＳによる米国特許出願（発明の名称「単離ナイーブ型多能性幹細胞およびそれを発生させる方法（ＩＳＯＬＡＴＥＤＮＡＩＶＥＰＬＵＲＩＰＯＴＥＮＴＳＴＥＭＣＥＬＬＳＡＮＤＭＥＴＨＯＤＳＯＦＧＥＮＥＲＡＴＩＮＧＳＡＭＥ）」）（代理人事件記録簿第５８８７０号）にも関する。上記出願の内容はその全体を参考として本明細書に組み込む。
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116286663A (zh) * 2016-06-21 2023-06-23 詹森生物科技公司 人多能干细胞衍生的功能性β细胞的体外产生
CN113069440A (zh) * 2021-04-08 2021-07-06 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基
WO2022213502A1 (zh) * 2021-04-08 2022-10-13 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基

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WO2017144695A1 (en) 2017-08-31
EP3420073C0 (en) 2023-11-08
MA45747A (fr) 2019-01-02
JP2019510482A (ja) 2019-04-18
WO2017144695A9 (en) 2017-10-19
US20190085295A1 (en) 2019-03-21
EP3420073A1 (en) 2019-01-02
JP7123802B2 (ja) 2022-08-23
EP3420073B1 (en) 2023-11-08
ES2967942T3 (es) 2024-05-06
US11274280B2 (en) 2022-03-15

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