CN108699515A - 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 - Google Patents

由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 Download PDF

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CN108699515A
CN108699515A CN201780013255.3A CN201780013255A CN108699515A CN 108699515 A CN108699515 A CN 108699515A CN 201780013255 A CN201780013255 A CN 201780013255A CN 108699515 A CN108699515 A CN 108699515A
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cells
pluripotent stem
derived
endocrine progenitor
human pluripotent
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Chinese (zh)
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N.S.克里斯特弗森
U.多恩
M.汉森
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Novo Nordisk AS
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/845—Gamma amino butyric acid [GABA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
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  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
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  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN201780013255.3A 2016-02-24 2017-02-24 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞 Pending CN108699515A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16157181 2016-02-24
EP16157181.5 2016-02-24
PCT/EP2017/054390 WO2017144695A1 (en) 2016-02-24 2017-02-24 Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors

Publications (1)

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CN108699515A true CN108699515A (zh) 2018-10-23

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CN201780013255.3A Pending CN108699515A (zh) 2016-02-24 2017-02-24 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞

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US (1) US11274280B2 (https=)
EP (1) EP3420073B1 (https=)
JP (1) JP7123802B2 (https=)
CN (1) CN108699515A (https=)
ES (1) ES2967942T3 (https=)
MA (1) MA45747A (https=)
WO (1) WO2017144695A1 (https=)

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CN113069440A (zh) * 2021-04-08 2021-07-06 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基
CN116286663A (zh) * 2016-06-21 2023-06-23 詹森生物科技公司 人多能干细胞衍生的功能性β细胞的体外产生

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CN120796168A (zh) 2013-06-11 2025-10-17 哈佛学院校长同事会 SC-β细胞以及用于产生其的组合物和方法
WO2015175307A1 (en) 2014-05-16 2015-11-19 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
CN108699515A (zh) 2016-02-24 2018-10-23 诺和诺德股份有限公司 由人多能干细胞衍生的内分泌祖细胞生成功能性β细胞
KR102738995B1 (ko) 2017-09-11 2024-12-06 노보 노르디스크 에이/에스 시험관 내 줄기 세포로부터 유래된 nkx6.1 및 c-펩티드 공발현 세포의 농축
EP3710021A4 (en) 2017-11-15 2021-08-11 Semma Therapeutics, Inc. ISLAND CELL MANUFACTURING COMPOSITIONS AND METHOD OF USE
EP3758719A4 (en) * 2018-03-02 2021-12-08 Vertex Pharmaceuticals Inc. METHODS FOR IMPROVING THE DIFFERENTIATION OF STEM CELLS INTO BETA CELLS
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
WO2020043292A1 (en) * 2018-08-30 2020-03-05 Novo Nordisk A/S Generation of functional beta cells from human pluripotent stem cell-derived endocrine progenitors
US20220177849A1 (en) 2019-04-08 2022-06-09 Novo Nordisk A/S Generation of pancreatic endoderm from stem cell derived definitive endoderm
TW202115245A (zh) 2019-06-27 2021-04-16 丹麥商諾佛 儂迪克股份有限公司 安全免疫隱形細胞
CN114729323B (zh) 2019-11-22 2024-09-17 诺和诺德股份有限公司 旋转聚集的神经微球及其应用
WO2021174201A1 (en) * 2020-02-28 2021-09-02 The Trustees Of The University Of Pennsylvania Tissue culture media for survival and engraftment of human pancreatic islets
WO2021175768A1 (en) 2020-03-02 2021-09-10 Novo Nordisk A/S Use of pluripotent markers to detect contaminating residual undifferentiated pluripotent stem cells
US20230313145A1 (en) 2020-08-28 2023-10-05 Novo Nordisk A/S Method for screening in vitro population of stem cell derived beta like cells and novel markers thereof
WO2022129472A1 (en) 2020-12-18 2022-06-23 Novo Nordisk A/S Safe immuno-stealth cells
WO2022136215A1 (en) 2020-12-21 2022-06-30 Novo Nordisk A/S Safe immuno-stealth cells
EP4423254A4 (en) * 2021-10-28 2026-01-14 Memorial Sloan Kettering Cancer Center COMPOSITIONS AND PROCESSES TO PROMOTE IN VITRO CELL MATURATION
WO2023118050A1 (en) 2021-12-21 2023-06-29 Novo Nordisk A/S Use of novel markers to detect pluripotent stem cells
WO2024151541A1 (en) 2023-01-09 2024-07-18 Sana Biotechnology, Inc. Type-1 diabetes autoimmune mouse
EP4716541A2 (en) 2023-05-22 2026-04-01 Sana Biotechnology, Inc. Methods of delivery of islet cells and related methods
WO2025093467A1 (en) 2023-10-31 2025-05-08 Novo Nordisk A/S Methods for preventing unwanted cells arising during differentiation of human pluripotent stem cells

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Publication number Priority date Publication date Assignee Title
CN116286663A (zh) * 2016-06-21 2023-06-23 詹森生物科技公司 人多能干细胞衍生的功能性β细胞的体外产生
CN113069440A (zh) * 2021-04-08 2021-07-06 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基
WO2022213502A1 (zh) * 2021-04-08 2022-10-13 澳门大学 甲状腺素的新的应用、培养多能干细胞的方法和培养基

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Publication number Publication date
EP3420073C0 (en) 2023-11-08
EP3420073A1 (en) 2019-01-02
JP7123802B2 (ja) 2022-08-23
JP2019510482A (ja) 2019-04-18
MA45747A (fr) 2019-01-02
WO2017144695A9 (en) 2017-10-19
ES2967942T3 (es) 2024-05-06
US20190085295A1 (en) 2019-03-21
US11274280B2 (en) 2022-03-15
EP3420073B1 (en) 2023-11-08
WO2017144695A1 (en) 2017-08-31

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