CN108685711A - A kind of bacterial cellulose facial mask and preparation method thereof that the lipid of load essential oil is modifies - Google Patents
A kind of bacterial cellulose facial mask and preparation method thereof that the lipid of load essential oil is modifies Download PDFInfo
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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Abstract
The present invention provides a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil, includes the following steps:Lecithin, cholesterol and vitamin E are added in ethyl alcohol, after being stirred until homogeneous, rotary evaporation removes ethyl alcohol, and citrate buffer solution, heating stirring is then added, and filtering obtains liposome vectors;Essential oil solution is added while vibrating in liposome vectors, regulation system to alkalescent, heating oscillation obtains the liposomic microcapsule of cladding essential oil;Aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture fluid, the liposomic microcapsule for coating essential oil is added in seed culture fluid, after mixing, the culture medium containing graphene oxide as carbon source, culture is added, washing sterilizing, obtains the modifies bacteria cellulose film of lipid;By the modifies bacteria cellulose film multiple-layer stacked of lipid, pressurization compacting obtains the modifies bacterial cellulose facial mask of the lipid of load essential oil.
Description
Technical field
The invention belongs to daily cosmetics technical fields, and in particular to a kind of bacterium that the lipid of load essential oil is modifies is fine
Tie up vegetarian noodles film and preparation method thereof.
Background technology
Bacteria cellulose is a kind of natural nano meter biomaterial, closely similar with native cellulose chemical composition, is all
The high-molecular compound being formed by connecting with β-Isosorbide-5-Nitrae-glycosidic linkage by glucose, be with acetobacter xylinum under the action of glucokinase
Conversion polymerization formed, have unique texture, have high water absorption, high-moisture-retention, high gas-liquid transmitance, high wet strength and
Can under hygrometric state the excellent properties such as in situ machine-shaping, have good application prospect in cosmetic field.
Purposes of the bacterial cellulose wet-coating in hair products disclosed in Chinese patent CN 102188330B, by zinc sulfate,
Vitamin B6/H, tea-seed oil/avocado oil/macadamia nut oil vegetable oil, hexadecyltrimethylammonium chloride/double tridecyl dimethyl chloride
Change ammonium/stearyl trimethyl chlorination ammonium surfactant, Arlacel-60/Tween-60 emulsifier, aloe water extract/ginseng water to carry
Object/tuber of multiflower knotweed water extract composition hair care active constituent, then adsorbs hair care active constituent, then bacterium is fine by bacterial cellulose wet-coating
The plain wet film of dimension is covered in hair hair-waving or hair dye on hair, and bacteria cellulose is utilized to have the spy of high retention ability
Property, can persistently keep the skin wet to hair and nutrition, improve the healthy glow and vigor of hair quality.Chinese patent CN
A kind of preparation method of collagen-modified bacteria cellulose compound film, surface is removed by bacteria cellulose film disclosed in 102352051B
Culture medium and impurity, after purification through sodium hydroxide activation process and with distilled water, through TEMPO/ periodic acid/periodate/dioxy
Change the oxidation systems such as nitrogen and the hydroxyl on bacteria cellulose is oxidized to carboxyl or aldehyde radical, then takes off bacteria cellulose through pressure
Collagen solution is coated on bacterial fibers film surface, forms collagen-modified bacteria cellulose film, be finally dried and dehydrated by water
To the collagen-modified bacteria cellulose compounded wet film of different moisture content.Collagen-modified bacteria cellulose prepared by this method is compound wet
Film can promote the recovery of collagen content in defect skin, so that bacteria cellulose is had first on excellent biocompatibility and bed
The ability of paying, and the collagen residue in bacteria cellulose composite membrane passes through imines with the functionalization group in bacteria cellulose
The chemical bonds such as key keep the combination of collagen and bacteria cellulose more secured, and discharge collagen by the fracture of imine linkage,
Achieve the purpose that skin care.By the above-mentioned prior art it is found that bacterial fibers can be assigned by being modified modification to bacteria cellulose
It is plain more functional, it is allowed to be more advantageous to the application in cosmetic field.
Invention content
The technical problem to be solved in the present invention is to provide a kind of bacterial cellulose facial masks that the lipid of load essential oil is modifies
And preparation method thereof, essential oil is embedded in the liposomic microcapsule that load essential oil is formed in liposome, then the fat that essential oil will be loaded
Plastid microcapsules are added in the preparation process of bacteria cellulose film, bacteria cellulose are formed on the surface of liposome, by lipid
Body coats completely, forms the modifies bacteria cellulose film of lipid, finally folds the bacteria cellulose film that multilamellar liposome is modified
Pressurization system obtains the modifies bacterial cellulose facial mask of the lipid of load essential oil.
In order to solve the above technical problems, the technical scheme is that:
A kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil, includes the following steps:
(1) lecithin, cholesterol and vitamin E are added in ethyl alcohol, after being stirred until homogeneous, rotary evaporation removes ethyl alcohol,
Then citrate buffer solution, heating stirring is added, filter at low temperature obtains liposome vectors;
(2) essential oil solution is added while vibrating in the liposome vectors of step (1) preparation, regulation system to weak base
Property, heating oscillation obtains the liposomic microcapsule of cladding essential oil;
(3) aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture
The liposomic microcapsule of cladding essential oil prepared by step (2) is added in seed culture fluid liquid, after mixing, is added oxygen-containing
Culture medium of the graphite alkene as carbon source, culture, washing sterilizing obtain the modifies bacteria cellulose film of lipid;
(4) by the modifies bacteria cellulose film multiple-layer stacked of the lipid of step (3) preparation, pressurization compacting is loaded
The modifies bacterial cellulose facial mask of the lipid of essential oil.
As a preferred embodiment of the above technical solution, in the step (1), the mass ratio of lecithin, cholesterol and vitamin E is
1:0.2-0.3:0.003-0.005。
As a preferred embodiment of the above technical solution, in the step (1), the temperature of heating stirring is 60-65 DEG C, and rotating speed is
50-200r/min, time 10-15min.
As a preferred embodiment of the above technical solution, in the step (2), essential oil is butter fruit essential oil, rose in essential oil solution
One or several kinds in essential oil, Seed of Camellia Sinensis essential oil, passion fruit essential oil, grape pip essential oil, pine tree essence oil, lemon, it is described
The content of essential oil is 0.5-5wt%.
As a preferred embodiment of the above technical solution, in the step (2), the technique for heating oscillation is:It is first 7.5-8 in pH
Under, 1-5min is handled with 40-50 DEG C and 100-150r/min of rotating speed, then again under 8-8.5, with 55-65 DEG C and 50-100r/
The rotating speed of min handles 5-10min.
As a preferred embodiment of the above technical solution, in the step (3), the liposomic microcapsule and seed culture of essential oil are coated
The mass ratio of liquid is 1:5-10.
As a preferred embodiment of the above technical solution, in the step (3), the temperature of culture is 30-32 DEG C, time 5-7d.
As a preferred embodiment of the above technical solution, in the step (3), essence is coated in the modifies bacteria cellulose film of lipid
The liposomic microcapsule of oil is embedded by bacteria cellulose.
As a preferred embodiment of the above technical solution, in the step (4), the modifies bacteria cellulose of the lipid of essential oil is loaded
The thickness of the modifies bacteria cellulose film of lipid is 0.8-1.5mm in facial mask.
A kind of modifies bacterial cellulose facial mask of lipid of load essential oil described above is also claimed in the present invention
The modifies bacterial cellulose facial mask of lipid of load essential oil prepared by preparation method.
Compared with prior art, the invention has the advantages that:
(1) the modifies bacterial cellulose facial mask of the lipid of load essential oil that prepared by the present invention include essential oil, liposome and
Bacteria cellulose, liposome have the bilayer lipid membrane structure of similar cell device film, are a kind of colloidal ions, good biocompatibility,
It can be fully absorbed by human body, but stability is poor, essential oil is embedded in the lipid that load essential oil is formed in liposome by the present invention
Body microcapsules are the loose of the bilayer side of liposome in the technique of preparation, are conducive to the infiltration of essential oil, then through low temperature
Processing is that liposome is closed again, and essential oil is firmly coated wherein, then bacterium is added in the liposomic microcapsule for loading essential oil
In the preparation process of cellulose membrane, bacteria cellulose is formed on the surface of liposome, liposome is coated completely, forms lipid
Modifies bacteria cellulose film improves the stability of liposome and essential oil in facial mask by double shielding, finally will be more
The modifies bacteria cellulose film superposition compacting of layer lipid, obtains the modifies bacterial cellulose facial mask of the lipid of load essential oil.
The modifies bacterial cellulose facial mask of lipid of load essential oil prepared by the present invention is during preparation, a small amount of liposomal fraction
Expose bacteria cellulose surface, most of liposome is coated wherein by bacteria cellulose, reduces facial mask and transported in packing box
The loss of active principle in journey, using preceding or in the process, by the liposome slow release of suitable swage load essential oil,
Into the surface layer and inside of skin, profound nourishing is carried out to skin, improves the utilization rate of active principle in facial mask, improve
The using effect of facial mask.
(2) present invention utilizes double-coating technology, and essential oil, liposome and bacteria cellulose are coated layer by layer, are prepared
The mechanical strength of the facial mask acted on layer by layer, facial mask is good, good stability of the dimension, for the profit of permanent sustained release and raising essential oil and liposome
Expenditure provides guarantee, and preparation method is simple, and controllability is strong, can take the circumstances into consideration to customize according to individual's skin situation.
Specific implementation mode
Below in conjunction with specific embodiment, the present invention will be described in detail, herein illustrative examples and explanation of the invention
For explaining the present invention, but it is not as a limitation of the invention.
Embodiment 1:
(1) it is 1 by mass ratio:0.2:0.003 lecithin, cholesterol and vitamin E is added in ethyl alcohol, is stirred until homogeneous
Afterwards, rotary evaporation removes ethyl alcohol, and the citrate buffer solution of 3 volumes times is then added, and is heated with the rotating speed of 50r/min at 60 DEG C
10min is stirred, the filter at low temperature at 0 DEG C obtains liposome vectors.
(2) the essential oil solution of the essential oil of butter fruit containing 0.5wt% and Rosa Damascana is added to liposome load while vibrating
In body, regulation system to alkalescent handles 1min first in the case where pH is 7.5 with the rotating speed of 40 DEG C and 100r/min, then again under 8,
5min is handled with 55 DEG C and 50r/min of rotating speed, obtains the liposomic microcapsule of cladding essential oil.
(3) aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture
Liquid is 1 according to the liposomic microcapsule of cladding essential oil and the mass ratio of seed culture fluid:5, the micro- glue of liposome that essential oil will be coated
Capsule is added in seed culture fluid, after mixing, the culture medium containing graphene oxide as carbon source is added, is cultivated at 30 DEG C
5d, washing sterilizing, obtains the modifies bacteria cellulose film of lipid.
(4) by the modifies bacteria cellulose film multiple-layer stacked of lipid, the pressurization compacting 1s at 0.1MPa, obtaining thickness is
The modifies bacterial cellulose facial mask of lipid of the load essential oil of 0.8mm.
Embodiment 2:
(1) it is 1 by mass ratio:0.3:0.005 lecithin, cholesterol and vitamin E is added in ethyl alcohol, is stirred until homogeneous
Afterwards, rotary evaporation removes ethyl alcohol, and the citrate buffer solution of 5 volumes times is then added, is added with the rotating speed of 200r/min at 65 DEG C
Thermal agitation 15min, filter at low temperature, obtains liposome vectors at 5 DEG C.
(2) the essential oil solution of the essential oil of Seed of Camellia Sinensis containing 5wt%, passion fruit essential oil, grape pip essential oil is added while vibrating
Enter in liposome vectors, regulation system to alkalescent, first in the case where pH is 8,5min is handled with 50 DEG C and 150r/min of rotating speed, so
Afterwards again under 8.5,10min is handled with 65 DEG C and 100r/min of rotating speed, obtains the liposomic microcapsule of cladding essential oil.
(3) aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture
Liquid is 1 according to the liposomic microcapsule of cladding essential oil and the mass ratio of seed culture fluid:10, the liposome for coating essential oil is micro-
Capsule is added in seed culture fluid, after mixing, the culture medium containing graphene oxide as carbon source is added, is cultivated at 32 DEG C
7d, washing sterilizing, obtains the modifies bacteria cellulose film of lipid.
(4) by the modifies bacteria cellulose film multiple-layer stacked of lipid, the pressurization compacting 5s at 0.5MPa, obtaining thickness is
The modifies bacterial cellulose facial mask of lipid of the load essential oil of 1.5mm.
Embodiment 3:
(1) it is 1 by mass ratio:0.25:0.004 lecithin, cholesterol and vitamin E is added in ethyl alcohol, stirs to equal
After even, rotary evaporation removes ethyl alcohol, the citrate buffer solution of 4 volumes times is then added, with the rotating speed of 100r/min at 63 DEG C
Heating stirring 13min, the filter at low temperature at 3 DEG C, obtains liposome vectors.
(2) the essential oil solution of pine tree essence oil containing 3wt%, lemon is added while vibrating in liposome vectors,
Regulation system is to alkalescent, and first in the case where pH is 7.8,3min is handled with 46 DEG C and 120r/min of rotating speed, then again under 8.3, with
60 DEG C and 80r/min of rotating speed handles 6min, obtains the liposomic microcapsule of cladding essential oil.
(3) aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture
Liquid is 1 according to the liposomic microcapsule of cladding essential oil and the mass ratio of seed culture fluid:7, the micro- glue of liposome that essential oil will be coated
Capsule is added in seed culture fluid, after mixing, the culture medium containing graphene oxide as carbon source is added, is cultivated at 31 DEG C
6d, washing sterilizing, obtains the modifies bacteria cellulose film of lipid.
(4) by the modifies bacteria cellulose film multiple-layer stacked of lipid, the pressurization compacting 2s at 0.4MPa, obtaining thickness is
The modifies bacterial cellulose facial mask of lipid of the load essential oil of 1.3mm.
Embodiment 4:
(1) it is 1 by mass ratio:0.2:0.005 lecithin, cholesterol and vitamin E is added in ethyl alcohol, is stirred until homogeneous
Afterwards, rotary evaporation removes ethyl alcohol, and the citrate buffer solution of 3 volumes times is then added, and is heated with the rotating speed of 50r/min at 65 DEG C
15min is stirred, the filter at low temperature at 0 DEG C obtains liposome vectors.
(2) the essential oil solution of the essential oil of butter fruit containing 5wt%, passion fruit essential oil, lemon is added while vibrating
In liposome vectors, regulation system to alkalescent handles 5min, so first in the case where pH is 7.5 with 50 DEG C and 100r/min of rotating speed
Afterwards again under 8,5min is handled with 65 DEG C and 100r/min of rotating speed, obtains the liposomic microcapsule of cladding essential oil.
(3) aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture
Liquid is 1 according to the liposomic microcapsule of cladding essential oil and the mass ratio of seed culture fluid:10, the liposome for coating essential oil is micro-
Capsule is added in seed culture fluid, after mixing, the culture medium containing graphene oxide as carbon source is added, is cultivated at 30 DEG C
7d, washing sterilizing, obtains the modifies bacteria cellulose film of lipid.
(4) by the modifies bacteria cellulose film multiple-layer stacked of lipid, the pressurization compacting 5s at 0.1MPa, obtaining thickness is
The modifies bacterial cellulose facial mask of lipid of the load essential oil of 1.3mm.
Embodiment 5:
(1) it is 1 by mass ratio:0.3:0.003 lecithin, cholesterol and vitamin E is added in ethyl alcohol, is stirred until homogeneous
Afterwards, rotary evaporation removes ethyl alcohol, and the citrate buffer solution of 5 volumes times is then added, is added with the rotating speed of 150r/min at 60 DEG C
Thermal agitation 11min, the filter at low temperature at 3 DEG C, obtains liposome vectors.
(2) liposome vectors are added while vibrating in the essential oil solution of the essential oil of passion fruit containing 1.5wt%, lemon
In, regulation system to alkalescent handles 3min first in the case where pH is 7.9 with the rotating speed of 43 DEG C and 120r/min, then again under 8.1,
7min is handled with 62 DEG C and 80r/min of rotating speed, obtains the liposomic microcapsule of cladding essential oil.
(3) aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture
Liquid is 1 according to the liposomic microcapsule of cladding essential oil and the mass ratio of seed culture fluid:9, the micro- glue of liposome that essential oil will be coated
Capsule is added in seed culture fluid, after mixing, the culture medium containing graphene oxide as carbon source is added, is cultivated at 31 DEG C
6.5d, washing sterilizing, obtains the modifies bacteria cellulose film of lipid.
(4) by the modifies bacteria cellulose film multiple-layer stacked of lipid, the pressurization compacting 2s at 0.4MPa, obtaining thickness is
The modifies bacterial cellulose facial mask of lipid of the load essential oil of 1.4mm.
Embodiment 6:
(1) it is 1 by mass ratio:0.27:0.0045 lecithin, cholesterol and vitamin E is added in ethyl alcohol, stirs to equal
After even, rotary evaporation removes ethyl alcohol, the citrate buffer solution of 4.5 volumes times is then added, with 50-200r/min's at 61 DEG C
Rotating speed heating stirring 13min, the filter at low temperature at 2 DEG C obtain liposome vectors.
(2) fat is added while vibrating in the essential oil solution of the essential oil of grape pip containing 2wt%, pine tree essence oil, lemon
In plasmid vector, regulation system to alkalescent handles 5min, then first in the case where pH is 7.8 with 47 DEG C and 100r/min of rotating speed
Again under 8,10min is handled with 65 DEG C and 50r/min of rotating speed, obtains the liposomic microcapsule of cladding essential oil.
(3) aseptically, acetobacter xylinum is inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture
Liquid is 1 according to the liposomic microcapsule of cladding essential oil and the mass ratio of seed culture fluid:6, the micro- glue of liposome that essential oil will be coated
Capsule is added in seed culture fluid, after mixing, the culture medium containing graphene oxide as carbon source is added, is cultivated at 30 DEG C
7d, washing sterilizing, obtains the modifies bacteria cellulose film of lipid.
(4) by the modifies bacteria cellulose film multiple-layer stacked of lipid, the pressurization compacting 1s at 0.5MPa, obtaining thickness is
The modifies bacterial cellulose facial mask of lipid of the load essential oil of 0.9mm.
The mechanical performance of the modifies bacterial cellulose facial mask of lipid for the load essential oil that after testing, prepared by embodiment 1-6,
Anti-microbial property, sustained release performance, the result of moisturizing rate and antioxygenic property are as follows:
As seen from the above table, the bacterial cellulose facial mask mechanical performance that the lipid for the load essential oil that prepared by the present invention is modifies is excellent
It is different, can be in short-term with long-acting skin care, using effect is good.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology can all carry out modifications and changes to above-described embodiment without violating the spirit and scope of the present invention.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil, which is characterized in that including following
Step:
(1) lecithin, cholesterol and vitamin E are added in ethyl alcohol, after being stirred until homogeneous, rotary evaporation removes ethyl alcohol, then
Citrate buffer solution, heating stirring is added, filtering obtains liposome vectors;
(2) essential oil solution is added while vibrating in the liposome vectors of step (1) preparation, regulation system to alkalescent,
Heating oscillation, obtains the liposomic microcapsule of cladding essential oil;
(3) aseptically, acetobacter xylinum being inoculated in fluid nutrient medium, uniform shaken cultivation obtains seed culture fluid,
The liposomic microcapsule of cladding essential oil prepared by step (2) is added in seed culture fluid, after mixing, oxygen-containing fossil is added
Culture medium of the black alkene as carbon source, culture, washing sterilizing obtain the modifies bacteria cellulose film of lipid;
(4) by the modifies bacteria cellulose film multiple-layer stacked of the lipid of step (3) preparation, pressurization compacting obtains load essential oil
The modifies bacterial cellulose facial mask of lipid.
2. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (1), the mass ratio of lecithin, cholesterol and vitamin E is 1:0.2-0.3:0.003-
0.005。
3. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (1), the temperature of heating stirring is 60-65 DEG C, rotating speed 50-200r/min, time 10-
15min。
4. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (2), essential oil is butter fruit essential oil, Rosa Damascana, Seed of Camellia Sinensis essential oil, hundred perfume in essential oil solution
The content of one or several kinds in fruit essential oil, grape pip essential oil, pine tree essence oil, lemon, the essential oil is 0.5-
5wt%.
5. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (2), the technique for heating oscillation is:First in the case where pH is 7.5-8, with 40-50 DEG C and 100-
The rotating speed of 150r/min handles 1-5min, and then again under 8-8.5,5- is handled with 55-65 DEG C and 50-100r/min of rotating speed
10min。
6. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (3), the mass ratio of the liposomic microcapsule and seed culture fluid that coat essential oil is 1:5-10.
7. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (3), the temperature of culture is 30-32 DEG C, time 5-7d.
8. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (3), the liposomic microcapsule that essential oil is coated in the modifies bacteria cellulose film of lipid is thin
Fungin embeds.
9. a kind of preparation method of the modifies bacterial cellulose facial mask of lipid of load essential oil according to claim 1,
It is characterized in that:In the step (4), modifies thin of lipid in the modifies bacterial cellulose facial mask of lipid of essential oil is loaded
The thickness of fungin film is 0.8-1.5mm.
10. a kind of preparation method system of the modifies bacterial cellulose facial mask of lipid of load essential oil described in claim 1-9
The modifies bacterial cellulose facial mask of the standby lipid for loading essential oil.
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| CN201810881143.6A CN108685711A (en) | 2018-08-04 | 2018-08-04 | A kind of bacterial cellulose facial mask and preparation method thereof that the lipid of load essential oil is modifies |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136874A (en) * | 2020-09-01 | 2020-12-29 | 浙江大学舟山海洋研究中心 | Modified protocatechuic acid liposome for storage and preservation of shrimps and crabs as well as preparation method and application thereof |
| CN113338074A (en) * | 2021-01-12 | 2021-09-03 | 刘秀娟 | Novel painting transparent oil rice paper and manufacturing method thereof |
-
2018
- 2018-08-04 CN CN201810881143.6A patent/CN108685711A/en not_active Withdrawn
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136874A (en) * | 2020-09-01 | 2020-12-29 | 浙江大学舟山海洋研究中心 | Modified protocatechuic acid liposome for storage and preservation of shrimps and crabs as well as preparation method and application thereof |
| CN112136874B (en) * | 2020-09-01 | 2022-11-01 | 浙江大学舟山海洋研究中心 | Modified protocatechuic acid liposome for storage and preservation of shrimps and crabs as well as preparation method and application of modified protocatechuic acid liposome |
| CN113338074A (en) * | 2021-01-12 | 2021-09-03 | 刘秀娟 | Novel painting transparent oil rice paper and manufacturing method thereof |
| CN113338074B (en) * | 2021-01-12 | 2022-11-15 | 刘秀娟 | Novel painting transparent oil rice paper and manufacturing method thereof |
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Application publication date: 20181023 |