CN108684716A - A kind of compounding improvement medicament and preparation method thereof for preventing lady's-grass - Google Patents
A kind of compounding improvement medicament and preparation method thereof for preventing lady's-grass Download PDFInfo
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- CN108684716A CN108684716A CN201810733062.1A CN201810733062A CN108684716A CN 108684716 A CN108684716 A CN 108684716A CN 201810733062 A CN201810733062 A CN 201810733062A CN 108684716 A CN108684716 A CN 108684716A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/66—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms
- A01N43/68—1,3,5-Triazines, not hydrogenated and not substituted at the ring nitrogen atoms with two or three nitrogen atoms directly attached to ring carbon atoms
- A01N43/70—Diamino—1,3,5—triazines with only one oxygen, sulfur or halogen atom or only one cyano, thiocyano (—SCN), cyanato (—OCN) or azido (—N3) group directly attached to a ring carbon atom
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Abstract
The invention discloses a kind of compoundings of prevention lady's-grass to improve medicament and preparation method thereof, the formula of the medicament is made of a certain proportion of activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum, sucrose, tween, atrazine, efficient fluazifop, wetting agent, dispersant, it is combined with chemical prevention medicament by biology, efficiently inhibits or kill the lady's-grass in rice terrace.The present invention uses a variety of auxiliary agents, improves the control effect of biological control agents, effectively reduces the usage amount of chemical pesticide, overcome the drug resistance of lady's-grass, to Security of rice height.
Description
Technical field
The present invention relates to technical field of agriculture science, more particularly to the compounding improvement medicament of a kind of prevention lady's-grass and its preparation
Method.
Background technology
China is maximum Rice Production state in the world, and paddy production history is long, and distributional region is wide, cultivates face every year
There is plantation in about 33,000,000 hectares of product, the whole nation in addition to Qinghai Province.Lady's-grass is distributed in worldwide malignant weed and China rice field
One of main weeds in paddy field most wide, harm is most heavy.
Grass family annual malignant weed when lady's-grass, is distributed widely in the torrid zone and Temperate Region in China, hazard rice, wheat, big
30 multiple kinds of crops such as wheat, corn and soybean is to be distributed one of most commonly used 18 kinds of malignant weeds in the world, and lady's-grass is to environment
Adaptability is extremely strong, and growth period is more consistent with crop, easily competes consumption soil fertility with crop.The harm of lady's-grass shows following several
A aspect:1, lady's-grass root system is more flourishing compared with crops, stronger to the absorbability of the nutrition such as soil, moisture and nutrient, growth
It is relatively rapid, cause corps nutrient supply in periphery serious deficient, so that growth is lagged, when serious also its normal growth can hinder
Or cause death;2, the competitive advantage of lady's-grass is stronger, distributed more widely, prevents and kill off the improper quality that can influence crops;3, horse
Tang can be used as the vector of certain plants pathogeny bacterium or pest and disease damage, so as to cause the morbidity of periphery plant;4, lady's-grass root system is also
Certain inhibitory compound can be secreted, periphery plant normal growth is inhibited.
At this stage in agricultural production, the use of biological weed killer can not also much replace the use of chemical herbicide, however
If it is significantly that can find one or more of herbicides that can be used in combination with pathogen and carry out weed control jointly.
Have been reported that the chemical herbicide of microbial herbicide and low dosage is used in compounding the weeding ratio that can effectively improve exclusive use,
It is insufficient that chemical herbicide defect in terms of weed control can also be made up, and the usage amount of herbicide can be reduced, to certain journey
The pollution that chemical herbicide brings environment is reduced on degree, and can also expand herbicide controlling spectrum, is changed host selection, is reduced weeding
Cost etc..
Mainly there are following 3 kinds to the harm prevention and control measure in rice field about prevention lady's-grass at present:First, farming measure is prevented and kill off:
In the fall or spring carries out rice field to plough deeply deep ploughing, and the underground rhizome of lady's-grass is exposed to native table, it is blocked to derive breeding;The
Two, establish or restore rice field-upland field rotation:It is disliked to effectively control rice field in order to effectively control the harm of rice field malignant weed lady's-grass, it can
It carries out rice field-upland field rotation and reduces lady's-grass radix, and adjust soil fertility and improvement soil, greatly reduce the occurrence quantity of regrowth;Third is changed
Learn chemical control:Aunar Lijin, paraquat, the chemical agents such as glyphosate are apparent to lady's-grass preventive effect.
The growth that thoroughly can not kill and block lady's-grass with rice field-upland field rotation is prevented and kill off in farming measure, can only reduce its harm, because
Its vitality is indomitable, can revive again when encountering appropraite condition, the growth of explosion type is presented, and brings huge economic damage
It loses, and chemical weed control then brings environmental pollution, destroys the ecological balance, and improper use is easy to cause poisoning to rice, causes
In production practice due to the use of improper the poisoning of herbicide can usually occur for the underproduction, and some poisoning are extremely serious.
Moreover, long-term excessively to also result in the serious drug resistance population rising of lady's-grass, resistance kind using chemical herbicide
Group continuously emerges, to increase weeding difficulty.All has there are common several main gramineous herbicides anti-in lady's-grass at present
(resistance to) property population.New weeding technique is needed to carry out substituted or supplemented current chemical herbicide.Biological weed killer has research because of it
Development cost is relatively low, environmental-friendly, to non-target organism safety, the advantages that not generating resistant weed, therefore next life in recent years
Object herbicide receives much attention.
Invention content
The technical problems to be solved by the invention:For current rice field prevent lady's-grass chemical means exist pollution environment,
Improper use causes the shortcomings that poisoning and the active low problem of biological control agents, the present invention that can effectively reduce chemical agent use
Amount, reduces environmental pollution, and improves biological control agents control effect, and to Security of rice height.
In order to solve the above technical problems, the present invention provides technical solution below:
A kind of compounding improvement medicament of prevention lady's-grass, passes through following raw material and compounds preparation:Activity from Curvularia eragrostidis 5
~15 parts, 10~20 parts of anthrax-bacilus, thick smooth 15~25 parts of fusarium oxysporum, 5~10 parts of sucrose, 10~20 parts of soybean oil, tween 5~
15 parts, 10~20 parts of atrazine, 10~20 parts of efficient fluazifop, 0.1~0.5 part of sodium metnylene bis-naphthalene sulfonate, bentonite 150
~250 parts.
Preferably, a kind of compounding of prevention lady's-grass improves medicament, is compounded and is prepared by following raw material:India lovegrass is curved
10 parts of spore mould, 15 parts of anthrax-bacilus, thick smooth 20 parts of fusarium oxysporum, 7.5 parts of sucrose, 15 parts of soybean oil, 10 parts of tween, atrazine 15
Part, 15 parts of efficient fluazifop, 0.3 part of sodium metnylene bis-naphthalene sulfonate, 200 parts of bentonite.
Preferably, the activity from Curvularia eragrostidis bacterial strain is QZ2000, and thick smooth fusarium oxysporum bacterial strain is ZC201301, anthrax
Bacteria strain is Col-68.
Preferably, the tween is the mixture of polysorbas20, polysorbate40, Tween 80, and weight ratio is:Polysorbas20:Tween
40:Tween 80=1:1:1;The atrazine is 30% atrazine wettable powder, and the efficient fluazifop is 10% smart pyrrole
Fluorine dogstail missible oil.
A kind of preparation method of compound drug according to the prevention lady's-grass in claim 4, includes the following steps:
(1) activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum are cultivated and harvests spore, is washed with aseptic double-distilled water
And spore is resuspended, adjustment spore concentration is activity from Curvularia eragrostidis 1 × 106~108/ mL, anthrax-bacilus 5 × 106~108/ mL, thickness
Smooth fusarium oxysporum 5 × 107~109/mL;
By Tween-20, Tween-40 and Tween-80 according to 1:1:1 weight ratio premix weighs each group according to formula precision
Point;
(2) after mixing activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum spore suspension, sucrose, soybean is added
Mixture 800rpm, 15min are sufficiently stirred mixing by oil, tween, efficient fluazifop, sodium metnylene bis-naphthalene sulfonate;
(3) after mixing, atrazine and bentonite is added, is sufficiently mixed in pestsides synthesis machine to obtain the final product.
Preferably, the well-mixed condition is 5~20rpm at room temperature using vacuum pestsides synthesis machine, mixing 5~
20min。
The advantageous effect that the present invention obtains:
The preferred embodiment that the present invention uses to the lady's-grass in rice terrace have fabulous control effect, be added a variety of auxiliary agents at
Point, the prevention efficiency of biological agent is promoted, and chemical agent usage amount significantly reduces, and is not damaged to ecological environment, no dirt
Dye.Using the method for biological and chemical control composite reagent, solves lady's-grass and single chemistry or fungal agents generation resistance are asked
Topic, works along both lines, and rice field lady's-grass is killed in multiple means combination, and strain preventive effect is close to 100%, while thoroughly removing lady's-grass within 28 days
To rice without any infection or inhibiting effect, the yield and quality of rice is improved.
Specific implementation mode
Below by the description to embodiment, specific embodiments of the present invention will be described in further detail, with side
Those skilled in the art is helped to have more complete, accurate and deep understanding to the inventive concept of the present invention, technical solution.
Embodiment 1:Medicament is prepared as follows
10 parts of activity from Curvularia eragrostidis QZ2000,15 parts of anthrax-bacilus Col-68, thick smooth fusarium oxysporum ZC201301 20
Part, 7.5 parts of sucrose, 15 parts of soybean oil, 10 parts of tween, 15 parts of 30% atrazine wettable powder, 10% efficient fluazifop breast
15 parts of oil, 0.3 part of sodium metnylene bis-naphthalene sulfonate, 200 parts of bentonite.
Preparation steps are as follows:
(1) preparation of thick smooth fusarium oxysporum spore suspension:Thick smooth fusarium oxysporum ZC201301 strains are inoculated in PDA plate
On culture medium, 26 DEG C are protected from light culture 8 days, are washed down the conidium of media surface with sterile water, 5000rpm, centrifuge 10min
Spore is collected, is used in combination sterile water to be resuspended, counts up to 5 × 108Conidial suspension is made in/mL;
(2) preparation of activity from Curvularia eragrostidis spore suspension:Activity from Curvularia eragrostidis QZ2000 strains are inoculated in PDA to put down
On plate culture medium, 28 DEG C are protected from light culture 12 days, are washed down the conidium of media surface with sterile water, 5000rpm, centrifuge
10min collects spore, is used in combination sterile water to be resuspended, counts up to 1 × 107Conidial suspension is made in/mL;
(3) picking be stored in inclined-plane anthrax-bacilus Col-68 strains it is a little, be inoculated in PSA tablets.In 28 DEG C of dark culturings
After 4d, the mycelia block of 3 pieces of a diameter of 5mm around picking colony, being inoculated into bran mass, (30g/mL wheat bran small fires, which disappear, boils
The filtrate of 30min), after 28 DEG C, 160rpm, shaken cultivation 3d, double-layer filter paper, which is collected by filtration, embraces son, is resuspended, is counted with sterile water
To 5 × 107Conidial suspension is made in/mL;
(4) by Tween-20, Tween-40 and Tween-80 according to 1:1:1 weight ratio premix, weighs respectively according to formula precision
Component;
(5) after mixing activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum spore suspension, sucrose, soybean is added
Oil, tween, 10% efficient fluazifop missible oil, sodium metnylene bis-naphthalene sulfonate, are sufficiently stirred mixed by mixture 800rpm, 15min
Even suspension mixing;
(6) after mixing, after mixing, 30% atrazine wettable powder and bentonite is added, in vacuum pestsides synthesis machine,
5~20rpm of room temperature, 5~20min of mixing to obtain the final product.
Embodiment 2:It is formulated as follows the compound drug of ingredient (sucrose, soy meal, the concentration of tween are preferred):
10 parts of activity from Curvularia eragrostidis QZ2000,15 parts of anthrax-bacilus Col-68, thick smooth fusarium oxysporum ZC201301 20
It is part, 15 parts of 30% atrazine wettable powder, 15 parts of 10% efficient fluazifop missible oil, 0.3 part of sodium metnylene bis-naphthalene sulfonate, swollen
200 parts of profit soil.
Sucrose is set, gradient is set:5,6,7,8,9,10 parts by weight;
Soybean oil is set, gradient is set:10,12,14,16,18,20 parts by weight;
Tween is set, gradient is set:5,6.5,8,11.5,13.5,15 parts by weight;
Compound drug preparation method is the same as embodiment 1.
Compound drug semilate rice Bipolaris and palace portion cochliobolus in comparing embodiment 1 and embodiment 2 infect lady's-grass
The promotion effect of rate:
The medicament of above-mentioned preparation is pressed 1:300 are diluted with water into suspension, are placed in turbine mixer and mix 10 seconds.Use scissors
75% alcoholic solution of several pieces lady's-grass blades of clip sterilizes 10s, and aqua sterilisa rinses 3 times.Immersion 1h in above each processing is placed in take
Go out, be placed in the sterilizes culture dish for being covered with sterilizing filter paper, adds aqua sterilisa 5mL moisturizings.Respectively processing is repeated 3 times each bacterial strain, is often repeated
6 blades are handled, preservative film seals and punches ventilation.Morbidity percentage is investigated after being integrally placed at 28 DEG C of constant incubator culture 5d
Rate, grade scale is shown in document (Hong Jianming, 2006), as a result as follows:
Table 1 combines activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum under different auxiliary agents to the pathogenicity rate of lady's-grass
As shown in Table 1 under the optimum condition of embodiment 1, activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum spore
Maximum is reached to the pathogenicity rate of lady's-grass, compared with blank control CK, pathogenic infection rate is substantially improved, sucrose, soybean oil, tween etc.
The addition of biological assistant increases the biological control activity of activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum spore, for sense
It contaminates lady's-grass and advantage is provided.
Embodiment 3:It is formulated as follows the compound drug of ingredient
5 parts of activity from Curvularia eragrostidis, 10 parts of anthrax-bacilus, thick smooth 15 parts of fusarium oxysporum, 5 parts of sucrose, 10 parts of soybean oil, tween
5 parts, 10 parts of atrazine, 10 parts of efficient fluazifop, 0.1 part of sodium metnylene bis-naphthalene sulfonate, 150 parts of bentonite.
Preparation steps are as follows:
(1) preparation of thick smooth fusarium oxysporum spore suspension:Thick smooth fusarium oxysporum ZC201301 strains are inoculated in PDA plate
On culture medium, 27 DEG C are protected from light culture 8 days, are washed down the conidium of media surface with sterile water, 5000rpm, centrifuge 10min
Spore is collected, is used in combination sterile water to be resuspended, counts up to 5 × 107Conidial suspension is made in/mL;
(2) preparation of activity from Curvularia eragrostidis spore suspension:Activity from Curvularia eragrostidis QZ2000 strains are inoculated in PDA to put down
On plate culture medium, 28 DEG C are protected from light culture 12 days, are washed down the conidium of media surface with sterile water, 5000rpm, centrifuge
10min collects spore, is used in combination sterile water to be resuspended, counts up to 1 × 106Conidial suspension is made in/mL;
(3) picking be stored in inclined-plane anthrax-bacilus Col-68 strains it is a little, be inoculated in PSA tablets.In 28 DEG C of dark culturings
After 4d, the mycelia block of 3 pieces of a diameter of 5mm around picking colony, being inoculated into bran mass, (30g/mL wheat bran small fires, which disappear, boils
The filtrate of 30min), after 28 DEG C, 160rpm, shaken cultivation 3d, double-layer filter paper, which is collected by filtration, embraces son, is resuspended, is counted with sterile water
To 5 × 106Conidial suspension is made in/mL;
(4) by Tween-20, Tween-40 and Tween-80 according to 1:1:1 weight ratio premix, weighs respectively according to formula precision
Component;
(5) after mixing activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum spore suspension, sucrose, soybean is added
Oil, tween, 10% efficient fluazifop missible oil, sodium metnylene bis-naphthalene sulfonate, are sufficiently stirred mixed by mixture 800rpm, 15min
Even suspension mixing;
(6) after mixing, after mixing, 30% atrazine wettable powder and bentonite is added, in vacuum pestsides synthesis machine,
Room temperature 5rpm, mixing 5min to obtain the final product.
Embodiment 4:It is formulated as follows the compound drug of ingredient
15 parts of activity from Curvularia eragrostidis, thick smooth 25 parts of fusarium oxysporum, 10 parts of sucrose, 20 parts of soybean oil, is spat 20 parts of anthrax-bacilus
15 parts of temperature, 20 parts of 30% atrazine wettable powder, 20 parts of 10% efficient fluazifop missible oil, sodium metnylene bis-naphthalene sulfonate 0.5
Part, 250 parts of bentonite.
Preparation steps are as follows:
(1) preparation of thick smooth fusarium oxysporum spore suspension:Thick smooth fusarium oxysporum ZC201301 strains are inoculated in PDA plate
On culture medium, 28 DEG C are protected from light culture 8 days, are washed down the conidium of media surface with sterile water, 5000rpm, centrifuge 10min
Spore is collected, is used in combination sterile water to be resuspended, counts up to 5 × 109Conidial suspension is made in/mL;
(2) preparation of activity from Curvularia eragrostidis spore suspension:Activity from Curvularia eragrostidis QZ2000 strains are inoculated in PDA to put down
On plate culture medium, 28 DEG C are protected from light culture 12 days, are washed down the conidium of media surface with sterile water, 5000rpm, centrifuge
10min collects spore, is used in combination sterile water to be resuspended, counts up to 1 × 108Conidial suspension is made in/mL;
(3) picking be stored in inclined-plane anthrax-bacilus Col-68 strains it is a little, be inoculated in PSA tablets.In 28 DEG C of dark culturings
After 4d, the mycelia block of 3 pieces of a diameter of 4mm around picking colony, being inoculated into bran mass, (30g/mL wheat bran small fires, which disappear, boils
The filtrate of 30min), after 28 DEG C, 200rpm, shaken cultivation 5d, double-layer filter paper, which is collected by filtration, embraces son, is resuspended, is counted with sterile water
To 5 × 108Conidial suspension is made in/mL;
(4) by Tween-20, Tween-40 and Tween-80 according to 1:1:1 weight ratio premix, weighs respectively according to formula precision
Component;
(5) after mixing activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum spore suspension, sucrose, soybean is added
Oil, tween, 10% efficient fluazifop missible oil, sodium metnylene bis-naphthalene sulfonate, are sufficiently stirred mixed by mixture 800rpm, 15min
Even suspension mixing;
(6) after mixing, after mixing, 30% atrazine wettable powder and bentonite is added, in vacuum pestsides synthesis machine,
Room temperature 20rpm, mixing 20min to obtain the final product.
The compounding improvement medicament of the present invention is as follows to the control effect of rice field lady's-grass:
Concrete operation step is as follows:
(1) after rice transplanting, when 5~6 leaf phase of lady's-grass, by the compound drug prepared in embodiment 1,3,4 according to 1:
200 times are diluted with water, and stir into uniform suspension after impregnating 25min, suspension is sprayed according to the dose uniformity of 20kg/667 ㎡
Liquid, dispenser handle the 2~4cm of layer that pour water afterwards for 24 hours, keep water layer 6d.
(2) positive control medicament is using scattered gram of enemy and 15% efficient fluazifop missible oil.
Enemy dissipates gram use 1:100 are diluted with water, and impregnate 20min or so, then stir into uniform suspension, according to
The dose uniformity of 40kg/667 ㎡ sprays suspension.Dispenser handles layer 5cm that pour water afterwards for 24 hours, keeps water layer 7d.15% smart pyrrole fluorine standing grain
Careless missible oil 70mL adds water 40kg/667 ㎡ routinely to use.Not dispenser blank control is set.It is anti-to investigate weeds by 14d, 28d after dispenser
Effect, 4 cells of often processing investigation, 5 repetition sampling points are investigated per cell.
The long-term rice cultivation in plot is tested, soil property is brunisolic soil, and pH 7.1, the content of organic matter is medium, pours facility.Rice
Kind is double osmanthus 1, and dispenser on July 4 is cloudy on the day of dispenser, and average daily temperature is 23.4 1 30.5 DEG C in 28d, day the highest temperature be
26.8~38.3 DEG C, Daily minimum temperature is 23.1~27.2 DEG C, and relative humidity is 62% one 96%.
CK0 indicates weeds strain number before blank district dispenser;CK1 indicates weeds strain number after blank district dispenser;Pt0 tables
Show weeds strain number before chemicals treatment district dispenser;Pt1 indicates weeds strain number after chemicals treatment district dispenser.
G0 indicates weeds fresh weight after blank district dispenser;G indicates weeds fresh weight after chemicals treatment district dispenser.
Experimental result is as follows:
The strain preventive effect of 14d, 28d and strain fresh weight preventive effect after 2 compound drug of table and comparison medicament medicine
Table 2 is the results show that embodiment 1 14 days strain preventive effects and strain fresh weight preventive effect after spraying and are being sprayed more than 80%
The latter 28 days strain preventive effects and strain fresh weight preventive effect to lady's-grass are applied close to 100%, are above 15% efficient fluazifop missible oil group and enemy
Gram group is dissipated, and action notable to the control effect of lady's-grass is rapid, and 15% efficient fluazifop missible oil group is anti-in 28 days weeds
Effect is below 14 days, illustrates that 15% efficient fluazifop missible oil of lady's-grass pair produces drug resistance, and embodiment 1,3,4 does not occur
The phenomenon that Weed-control effect reduces over time illustrates that the present invention still has inhibition or killing effect, dosage to drug resistance lady's-grass
Aspect, the substantially less than sprayed dose of 15% efficient fluazifop missible oil group.
Test result is as follows to Toxicity To Rice for the compounding improvement medicament of the present invention:
Concrete operation step is as follows:
(1) after rice transplanting, when 5~6 leaf phase of lady's-grass, by the compound drug prepared in embodiment 1,3,4 according to 1:
200 times are diluted with water, and stir into uniform suspension after impregnating 25min, suspension is sprayed according to the dose uniformity of 20kg/667 ㎡
Liquid, dispenser handle the 2~4cm of layer that pour water afterwards for 24 hours, keep water layer 6d.
(2) positive control medicament is using scattered gram of enemy and 15% efficient fluazifop missible oil.
Enemy dissipates gram use 1:100 are diluted with water, and impregnate 20min or so, then stir into uniform suspension, according to
The dose uniformity of 40kg/667 ㎡ sprays suspension.Dispenser handles layer 5cm that pour water afterwards for 24 hours, keeps water layer 7d.15% smart pyrrole fluorine standing grain
Careless missible oil 70mL adds water 40kg/667 ㎡ routinely to use.Not dispenser blank control is set.It is anti-to investigate weeds by 14d, 28d after dispenser
Effect, 4 cells of often processing investigation, 5 repetition sampling points are investigated per cell.
The long-term rice cultivation in plot is tested, soil property is brunisolic soil, and pH 7.1, the content of organic matter is medium, pours facility.Rice
Kind is double osmanthus 1, and dispenser on July 4 is cloudy on the day of dispenser, and average daily temperature is 23.4 1 30.5 DEG C in 28d, day the highest temperature be
26.8~38.3 DEG C, Daily minimum temperature is 23.1~27.2 DEG C, and relative humidity is 62% one 96%.
As a result as follows:
Safety effects of 3 compound drug of table to rice
By table 3 as it can be seen that 7d, 28d, 60d, test block rice do not occur poisoning, the life of rice after sprinkling compound drug
Long and yield is normal, and indices are better than 15% efficient fluazifop missible oil group and blank group, this is because to lady's-grass
Inhibit and killing causes the growth of rice and constant to be significantly improved.
In conclusion the present invention is superior to traditional chemical medicament and single bacterial strain to the strain preventive effect and strain fresh weight preventive effect of lady's-grass
Biological agent, it is notable to the inhibition of lady's-grass, and dosage be less than traditional chemical medicament, environment influence it is small;To rice
It is safe, do not occur rice injury symptoms using short-term after medicament and long-term observation, and have increasing action to rice yield,
The lady's-grass prevention that can be widely applied to rice field, has broad application prospects.
Above example is merely illustrative of the invention's technical idea, and protection scope of the present invention cannot be limited with this, every
According to technological thought proposed by the present invention, any change done on the basis of technical solution each falls within the scope of the present invention
Within;The technology that the present invention is not directed to can be realized by the prior art.
Claims (6)
1. a kind of compounding of prevention lady's-grass improves medicament, which is characterized in that compounded and prepared by following raw material:India lovegrass
5~15 parts of curved spore mould, 10~20 parts of anthrax-bacilus, thick smooth 15~25 parts of fusarium oxysporum, 5~10 parts of sucrose, soybean oil 10~20
Part, 5~15 parts of tween, 10~20 parts of atrazine, 10~20 parts of efficient fluazifop, sodium metnylene bis-naphthalene sulfonate 0.1~0.5
Part, 150~250 parts of bentonite.
2. improveing medicament according to a kind of compounding of prevention lady's-grass in claim 1, which is characterized in that pass through following raw material
It is prepared by compounding:10 parts of activity from Curvularia eragrostidis, 15 parts of anthrax-bacilus, thick smooth 20 parts of fusarium oxysporum, 7.5 parts of sucrose, 15 parts of soybean oil,
10 parts of tween, 15 parts of atrazine, 15 parts of efficient fluazifop, 0.3 part of sodium metnylene bis-naphthalene sulfonate, 200 parts of bentonite.
3. improveing medicament according to a kind of compounding of prevention lady's-grass in claim 1, it is characterised in that:The activity from Curvularia eragrostidis
Bacterial strain is QZ2000, and thick smooth fusarium oxysporum bacterial strain is ZC201301, and anthrax bacteria strain is Col-68.
4. improveing medicament according to a kind of compounding of prevention lady's-grass in claim 1, it is characterised in that:The tween be polysorbas20,
The mixture of polysorbate40, Tween 80, weight ratio are:Polysorbas20:Polysorbate40:Tween 80=1:1:1;The atrazine is 30%
Atrazine wettable powder, the efficient fluazifop are 10% efficient fluazifop missible oil.
5. a kind of preparation method of compound drug according to the prevention lady's-grass in claim 4, which is characterized in that including walking as follows
Suddenly:
(1) activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum are cultivated and harvests spore, is washed and is laid equal stress on aseptic double-distilled water
Outstanding spore, adjustment spore concentration are activity from Curvularia eragrostidis 1 × 106~108/ mL, anthrax-bacilus 5 × 106~108/ mL, thick smooth spore
Sickle-like bacteria 5 × 107~109/mL;
By Tween-20, Tween-40 and Tween-80 according to 1:1:1 weight ratio premix weighs each component according to formula precision;
(2) after mixing activity from Curvularia eragrostidis, anthrax-bacilus, thick smooth fusarium oxysporum spore suspension, sucrose is added, soybean oil, spits
Mixture 800rpm, 15min are sufficiently stirred mixing by temperature, efficient fluazifop, sodium metnylene bis-naphthalene sulfonate;
(3) after mixing, atrazine and bentonite is added, is sufficiently mixed in pestsides synthesis machine to obtain the final product.
6. according to a kind of preparation method of the compounding improvement medicament of prevention lady's-grass described in claim 5, it is characterised in that:It is described
Well-mixed condition is using vacuum pestsides synthesis machine, and 5~20rpm, mixes 5~20min at room temperature.
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