CN108676729A - A kind of culture media composition containing fermentation mother liquor and preparation method thereof and fermentation process - Google Patents
A kind of culture media composition containing fermentation mother liquor and preparation method thereof and fermentation process Download PDFInfo
- Publication number
- CN108676729A CN108676729A CN201810520716.2A CN201810520716A CN108676729A CN 108676729 A CN108676729 A CN 108676729A CN 201810520716 A CN201810520716 A CN 201810520716A CN 108676729 A CN108676729 A CN 108676729A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- mother liquor
- mycelium
- culture media
- media composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention provides a kind of culture media composition containing fermentation mother liquor and preparation method thereof and fermentation process, belongs to fermentation mother liquor and ferment again and utilize technical field.The mycelium dry weight of the Agricus blazei carried out liquid fermentation using culture medium of the present invention containing fermentation mother liquor and rich iron are compared with fermentation mother liquor and fermentation mother liquor and fermenation raw liquid mixing match each group, be significantly increased, and in cost, turn waste into wealth, environmental-friendly etc. also have a clear superiority.
Description
Technical field
The present invention relates to fermentation mother liquors is fermented again using technical field, containing fermentation mother liquor in particular to one kind
Culture media composition and preparation method thereof and fermentation process.
Background technology
With the progress of human civilization and the sustainable development of national economy, people increasingly pay attention to the health of itself.
Agricus blazei is also known as Brazilian mushroom or Brazilian mushroom, originates in the ground such as Brazil, Peru, belongs to food, medicine dual-purpose mushroom class.Ji Song
Fine and soft nutrition is extremely abundant, and mouthfeel is fabulous, the pure fresh perfume (or spice) of taste, and edible value is quite high.Protein composition includes 18 kinds of amino acid, human body
8 kinds of essential amino acids it is complete, also contain multivitamin and ergosterol.It is that the common people pay tribute to noble and Mikado in Japan
One of treasure is referred to as " precious beautiful in mushroom ".
Agricus blazei contains abundant iron substance and cancerocidal substance, and to enhancing human immunity, supplement, which special procures nutrition, to be had very well
Effect, contained by polysaccharide body compound, conditioning effect is apparently higher than other mushrooms, can promote T lymphocytes, auxiliary
The production capacity of T cell, interferon and interleukins, makes body immunity improve.Wherein mannosan is (outstanding to inhibiting tumour
It is ascites carcinoma), medical hemorrhoid complicated by anal fistula, enhancing energy, anti-angiocardiopathy etc. have the magical effect.Agricus blazei has anticancer, blood pressure lowering, U.S.
Hold beauty treatment, nourishing and strengthening Yin and Yang and adjust human immunity and other effects, anticancer effect is first of edible mushroom.In prevention and treatment tumour side
There is effect unique in face, is known as " last food on the rescue patient with advanced cancer earth ".There are many fructification of Agricus blazei contains
Natural polysaccharide component can improve the life quality of specific crowd.The product is in September, 1999 by U.S.'s food and drug control
Office is recognized as " improving the nutritional supplement of immunity ".Just because of having so tempting nutritive value and medical health care function, institute
To start edible Agricus blazei heat in Japan in recent years.
Fermentation mother liquor is a kind of harmful nontoxic industrial wastewater.With the gradually expansion of fermentation technique application range, China
The development of fermentation industry is also to be getting faster, therefore the governing problem of such industrial wastewater is also increasingly by the weight of people
Depending on.Related scientific research unit in China's has done a large amount of work in terms of the processing of fermentation industry waste water and recycling at present,
Main method produces organic and inorganic fertilizer and production livestock fodder additives, production biological pesticide, production life including the use of waste water
Object flocculant, formation biomass hydrogen energy source etc..However, value caused by these methods is few.So needing to find better
The approach that fermentation mother liquor recycles is made full use of and is had become a top priority for enterprise's higher income of generation.
The existing mother liquor that will generally produce of manufacturing enterprise not only wastes resource, while also increasing enterprise as sewage disposal
Sewage disposal expense and ambient pollution burden, and constrain the development of fermentation industry.Although manufacturing enterprise, scientific research institution and having
The universities and colleges of pass have all carried out a large amount of research to improvement.But all there are no ripe complete set technologys to answer both at home and abroad at present
For production practices.Major problem is that one-time investment is excessive or day-to-day operation expense is excessively high, most producers can not hold
By, it has to the long-term present situation for maintaining discharge beyond standards.
The yield of development with industry and production-scale expansion, fermentation mother liquor will be also continuously increased, if cannot obtain
To effectively appropriate processing, the economy and environmental pressure of enterprise will be increased, hinder the sound development of enterprise.
It as being produced using fermentation mother liquor is recycled, can not only reduce environmental pollution, but also waste profit may be implemented
With to generate huge economy and ecological benefits.
Therefore, a kind of environment-protective process of processing Agricus blazei fermentation mother liquor is studied, to reduce contaminated wastewater, reduce sewage disposal
Burden, increases the economic benefit of enterprise, improves the added value of industry to greatest extent, have important practical significance.
In consideration of it, the present invention provides a kind of culture media composition containing fermentation mother liquor and preparation method thereof and fermentation side
Method.
Invention content
The purpose of the present invention is to provide a kind of culture media compositions containing fermentation mother liquor and preparation method thereof and fermentation
Method.
In order to realize that the above-mentioned purpose of the present invention, spy use following technical scheme：
In a first aspect, the present invention provides a kind of culture media composition containing fermentation mother liquor, the culture containing fermentation mother liquor
Base composition includes fermentation mother liquor, yeast, further includes corn Ultramicro-powder.
Further, the yeast in the culture media composition containing fermentation mother liquor is broken wall ferrum-rich saccharomyces cerevisiae.
Further, fermentation mother liquor is the waste liquid after the fermentation of Mycelium in Agaricus blazei Murill liquid fermentation medium composition, Ji Song
Fine and soft mycelium liquid fermenation raw liquid by broken wall ferrum-rich saccharomyces cerevisiae is 0.6 part, glucose is 20 parts, 0.3 part of potassium dihydrogen phosphate and sulfuric acid
Magnesium is 0.2 part of composition.
Further, the culture media composition containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor, wherein every liter of fermentation mother liquor
Middle addition broken wall ferrum-rich saccharomyces cerevisiae is 0.06~0.18g, corn Ultramicro-powder is 10~30g.
Further, the culture media composition containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor, wherein every liter of fermentation mother liquor
Middle addition broken wall ferrum-rich saccharomyces cerevisiae is 0.09~0.15g, the corn Ultramicro-powder is 15~25g.
Further, in preferred embodiments of the present invention, the culture containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor
Base composition, wherein being added in every liter of fermentation mother liquor, broken wall ferrum-rich saccharomyces cerevisiae is 0.12g, the corn Ultramicro-powder is 20g.
Iron content is that the broken wall ferrum-rich saccharomyces cerevisiae of 8000mg/kg is bought on the market with other nutrient media components in the present invention,
Corn Ultramicro-powder grain size is at 10 μm hereinafter, Agricus blazei seed used in liquid fermentation is prepared according to a conventional method.
Second aspect, the present invention provide the preparation method of the above-mentioned culture media composition containing fermentation mother liquor comprising：
The production method of culture medium containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor, it is broken by being added in every liter of fermentation mother liquor
After 121 DEG C of 15~30min of sterilizing of culture media composition that wall ferrum-rich saccharomyces cerevisiae is 0.06~0.18g, corn Ultramicro-powder is 10~30g
Cooling to obtain the final product.
The third aspect, the present invention also provides a kind of rich iron Agricus blazei mycelia containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor
Body liquid fermentation method comprising：Agricus blazei seed is seeded to the above-mentioned liquid containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor
It in body culture medium, carries out liquid fermentation, 25~27 DEG C of temperature, air agitation, 3~15d of fermentation time, by cultured zymotic fluid
Filtering or centrifugation 2000~10000rpm processing, collect mycelium, by washing, being dried to obtain organic rich iron Agricus blazei mycelia
Body.
Further, in preferred embodiments of the present invention, Agricus blazei seed is seeded to above-mentioned containing Agricus blazei mycelia
It in the fluid nutrient medium of body fluid body fermentation mother liquor, carries out liquid fermentation, 26 DEG C of temperature, air agitation, fermentation time 7d will be cultivated
Good zymotic fluid centrifuges 4000rpm processing, collects mycelium, by washing, being dried to obtain organic rich iron Mycelium in Agaricus blazei Murill.
Compared with prior art, beneficial effects of the present invention include：
The mycelium dry weight of Agricus blazei and rich iron compared with prior art, are significantly increased.
Specific implementation mode
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
The feature and performance of the present invention are described in further detail with reference to embodiments：
Embodiment 1
It is added broken wall ferrum-rich saccharomyces cerevisiae 0.06g, corn Ultramicro-powder 30g in 1L Mycelium in Agaricus blazei Murill liquid fermentation mother liquors, 121 DEG C
Sterilize 30min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 25 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 2000rpm processing by fermentation time 15d, collects mycelium, organic by washing, being dried to obtain
Rich iron Mycelium in Agaricus blazei Murill.
Embodiment 2
It is added broken wall ferrum-rich saccharomyces cerevisiae 0.18g, corn Ultramicro-powder 10g in 1L Mycelium in Agaricus blazei Murill liquid fermentation mother liquors, 121 DEG C
Sterilize 15min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 27 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 10000rpm processing by fermentation time 3d, collects mycelium, organic by washing, being dried to obtain
Rich iron Mycelium in Agaricus blazei Murill.
Embodiment 3
It is added broken wall ferrum-rich saccharomyces cerevisiae 0.09g, corn Ultramicro-powder 25g in 1L Mycelium in Agaricus blazei Murill liquid fermentation mother liquors, 121 DEG C
Sterilize 20min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 25 DEG C of temperature, air agitation,
Cultured zymotic fluid is carried out plate compression with the pressure of 60kg, mycelium is collected, by washing, drying by fermentation time 5d
Obtain organic rich iron Mycelium in Agaricus blazei Murill.
Embodiment 4
It is added broken wall ferrum-rich saccharomyces cerevisiae 0.15g, corn Ultramicro-powder 15g in 1L Mycelium in Agaricus blazei Murill liquid fermentation mother liquors, 121 DEG C
Sterilize 20min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 26 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 5000rpm processing, mycelium is collected, by washing, being dried to obtain organic richness by fermentation time 7d
Iron Mycelium in Agaricus blazei Murill.
Embodiment 5
Broken wall ferrum-rich saccharomyces cerevisiae 0.12g is added in 1L Mycelium in Agaricus blazei Murill liquid fermentation mother liquors, the corn Ultramicro-powder is
20g, 121 DEG C of sterilizing 25min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 26 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 4000rpm processing, mycelium is collected, by washing, being dried to obtain organic richness by fermentation time 7d
Iron Mycelium in Agaricus blazei Murill.
Comparative example 1
1L Mycelium in Agaricus blazei Murill liquid fermentation stostes, 121 DEG C of sterilizing 25min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 26 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 4000rpm processing, mycelium is collected, by washing, being dried to obtain organic richness by fermentation time 7d
Iron Mycelium in Agaricus blazei Murill.
Comparative example 2
1L Mycelium in Agaricus blazei Murill liquid fermentation mother liquors, 121 DEG C of sterilizing 25min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 26 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 4000rpm processing, mycelium is collected, by washing, being dried to obtain organic richness by fermentation time 7d
Iron Mycelium in Agaricus blazei Murill.
Comparative example 3
1L is taken to be trained by the mixing that Mycelium in Agaricus blazei Murill liquid fermentation mother liquor and Mycelium in Agaricus blazei Murill liquid fermentation stoste form
Support base (mother liquor：Stoste=1：1), 121 DEG C of sterilizing 25min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 26 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 4000rpm processing, mycelium is collected, by washing, being dried to obtain organic richness by fermentation time 7d
Iron Mycelium in Agaricus blazei Murill.
Comparative example 4
1L is taken to be trained by the mixing that Mycelium in Agaricus blazei Murill liquid fermentation mother liquor and Mycelium in Agaricus blazei Murill liquid fermentation stoste form
Support base (mother liquor：Stoste=1：2), 121 DEG C of sterilizing 25min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 26 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 4000rpm processing, mycelium is collected, by washing, being dried to obtain organic richness by fermentation time 7d
Iron Mycelium in Agaricus blazei Murill.
Comparative example 5
1L is taken to be trained by the mixing that Mycelium in Agaricus blazei Murill liquid fermentation mother liquor and Mycelium in Agaricus blazei Murill liquid fermentation stoste form
Support base (mother liquor：Stoste=1：3), 121 DEG C of sterilizing 25min postcoolings.
Agricus blazei seed is seeded in above-mentioned fluid nutrient medium, is carried out liquid fermentation, 26 DEG C of temperature, air agitation,
Cultured zymotic fluid is centrifuged 4000rpm processing, mycelium is collected, by washing, being dried to obtain organic richness by fermentation time 7d
Iron Mycelium in Agaricus blazei Murill.
Beneficial effects of the present invention such as following table：
As can be seen from the above table, in each group embodiment of the present invention liquid is carried out using culture medium of the present invention containing fermentation mother liquor
The mycelium dry weight for the Agricus blazei that body ferments and rich iron and fermentation mother liquor and fermentation mother liquor and fermenation raw liquid mixing match
Each group is compared, and is significantly increased, and in cost, turn waste into wealth, environmental-friendly etc. also have a clear superiority.
Unspecified link is the common knowledge that those of ordinary skill in the art can voluntarily select in the present invention.Though
So, above the present invention is described in detail with a general description of the specific embodiments, but on basis of the invention
On, it can be made some modifications or improvements, this is obvious for those of ordinary skill in the art.Therefore, not
These modifications or improvements on the basis of deviation spirit of that invention, belong to the scope of protection of present invention.
Claims (10)
1. a kind of culture media composition containing fermentation mother liquor, which is characterized in that the culture medium combination containing fermentation mother liquor
Object includes fermentation mother liquor, yeast.
2. the culture media composition according to claim 1 containing fermentation mother liquor, which is characterized in that described female containing fermentation
The culture media composition of liquid further includes corn Ultramicro-powder.
3. the culture media composition according to claim 1 containing fermentation mother liquor, which is characterized in that the yeast is broken wall
Ferrum-rich saccharomyces cerevisiae.
4. the culture media composition according to claim 1 containing fermentation mother liquor, which is characterized in that the fermentation mother liquor is
Waste liquid after the fermentation of Mycelium in Agaricus blazei Murill liquid fermentation medium composition, Mycelium in Agaricus blazei Murill liquid fermentation stoste is by broken wall richness
Iron yeast is 0.6 part, glucose is 20 parts, 0.3 part of potassium dihydrogen phosphate and magnesium sulfate are 0.2 part of composition.
5. the culture media composition according to claim 2 containing fermentation mother liquor, which is characterized in that every liter of fermentation is female
It is added that the broken wall ferrum-rich saccharomyces cerevisiae is 0.06~0.18g, the corn Ultramicro-powder is 10~30g in liquid.
6. the culture media composition according to claim 5 containing fermentation mother liquor, which is characterized in that the broken wall richness iron ferment
Mother is 0.09~0.15g, the corn Ultramicro-powder is 15~25g.
7. the culture media composition according to claim 5 containing fermentation mother liquor, which is characterized in that the broken wall richness iron ferment
Mother is 0.12g, the corn Ultramicro-powder is 20g.
8. a kind of production method for the culture medium containing fermentation mother liquor according to claim 1~7 any one of them, feature exist
In 121 DEG C of sterilizing 15~30min postcoolings of the culture media composition containing fermentation mother liquor.
9. a kind of rich iron Mycelium in Agaricus blazei Murill liquid fermentation method containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor, feature exist
In, Agricus blazei seed is seeded in fluid nutrient medium as described in claim 6, is carried out liquid fermentation, temperature 25~27
DEG C, air agitation, 3~15d of fermentation time collects cultured filtering fermentation liquor or centrifugation 2000~10000rpm processing
Mycelium, by washing, being dried to obtain organic rich iron Mycelium in Agaricus blazei Murill.
10. a kind of rich iron Mycelium in Agaricus blazei Murill liquid fermentation method containing Mycelium in Agaricus blazei Murill liquid fermentation mother liquor, feature
It is, Agricus blazei seed is seeded in fluid nutrient medium as described in claim 6, is carried out liquid fermentation, 26 DEG C of temperature,
Cultured zymotic fluid is centrifuged 4000rpm processing, mycelium is collected, by washing, drying by air agitation, fermentation time 7d
Obtain organic rich iron Mycelium in Agaricus blazei Murill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810520716.2A CN108676729B (en) | 2018-05-28 | 2018-05-28 | Culture medium composition containing fermentation waste liquid, and preparation method and fermentation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810520716.2A CN108676729B (en) | 2018-05-28 | 2018-05-28 | Culture medium composition containing fermentation waste liquid, and preparation method and fermentation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108676729A true CN108676729A (en) | 2018-10-19 |
| CN108676729B CN108676729B (en) | 2022-05-03 |
Family
ID=63808452
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810520716.2A Active CN108676729B (en) | 2018-05-28 | 2018-05-28 | Culture medium composition containing fermentation waste liquid, and preparation method and fermentation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108676729B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110423699A (en) * | 2019-09-16 | 2019-11-08 | 汤原波巴布生物科技有限公司 | A kind of mycelium liquid fermentation medium composition of selenium-rich agaric and preparation method thereof and fermentation process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1339582A (en) * | 2001-09-29 | 2002-03-13 | 李宝健 | Large-scale deep liquid fermentation process for Jisongrong |
| CN102369838A (en) * | 2010-08-11 | 2012-03-14 | 宝生物工程株式会社 | Manufacturing Method Of Liquid Seed Fungus Of Mushroom |
| CN106190866A (en) * | 2016-08-11 | 2016-12-07 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of reuse method of Cordyceps fermented waste fluid |
-
2018
- 2018-05-28 CN CN201810520716.2A patent/CN108676729B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1339582A (en) * | 2001-09-29 | 2002-03-13 | 李宝健 | Large-scale deep liquid fermentation process for Jisongrong |
| CN102369838A (en) * | 2010-08-11 | 2012-03-14 | 宝生物工程株式会社 | Manufacturing Method Of Liquid Seed Fungus Of Mushroom |
| CN106190866A (en) * | 2016-08-11 | 2016-12-07 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of reuse method of Cordyceps fermented waste fluid |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110423699A (en) * | 2019-09-16 | 2019-11-08 | 汤原波巴布生物科技有限公司 | A kind of mycelium liquid fermentation medium composition of selenium-rich agaric and preparation method thereof and fermentation process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108676729B (en) | 2022-05-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102199054B (en) | Production method and culture medium formula of zinc-rich edible mushroom | |
| CN106072491B (en) | A kind of fructus arctii base Ganoderma lucidum mycelium powder and preparation method thereof | |
| CN103190576B (en) | Cordyceps militaris soybean and preparation method thereof | |
| CN103598012A (en) | Ganoderma lucidum mycelium and preparation method thereof | |
| CN103263448B (en) | Fermentation bacteria used for fermentation pretreatment to improve extraction of Ginkgo biloba L. leaf flavone and application | |
| CN103460994B (en) | Cordyceps militaris nanometer selenium and preparation method thereof | |
| CN103583236A (en) | Method for preparing fleckedflesh polypore mycelium | |
| CN105027970A (en) | Pleurotus tuber-regium culture medium rich in selenium and germanium and preparing method thereof | |
| CN107114758A (en) | A kind of ferment cordyceps sinensis D-mannitol powder and preparation method thereof | |
| CN102577839B (en) | Method for producing medicinal or medicine-food dual-purpose fruit body by utilizing yellow wine grains as solid culture medium | |
| CN105707851A (en) | Pleurotus eryngii enzyme and preparing method thereof | |
| CN103421861B (en) | The method of Cordyceps Polysaccharide produced by a kind of liquid state fermentation rice bran wheat bran complete feed | |
| CN102771857A (en) | Truffle health-care drink prepared through liquid submerged fermentation and preparation method of truffle health-care drink prepared through liquid submerged fermentation | |
| CN110423699A (en) | A kind of mycelium liquid fermentation medium composition of selenium-rich agaric and preparation method thereof and fermentation process | |
| CN103299824A (en) | Method for preparing phellinus igniarius sclertium from fresh, tender and sweet corn | |
| CN104429622A (en) | Method for producing shiitake mushroom mycelia by using ginkgo leaves | |
| CN104522750A (en) | Alga healthcare food | |
| CN101731357A (en) | Method for preparing wormgrass soymilk | |
| CN103299823A (en) | Method for improving yield and trace element enrichment of liquid fermentation mycelium of lucid ganoderma | |
| CN108383652A (en) | Agricultural wastes cultivate rich selenium germanium green organic element nutrition fertilizer | |
| CN108676729A (en) | A kind of culture media composition containing fermentation mother liquor and preparation method thereof and fermentation process | |
| CN108477595A (en) | A kind of preparation method and equipment therefor of mushroom enzyme food additive | |
| CN107382592A (en) | A kind of white fungus matrix and preparation method thereof | |
| CN103229666A (en) | Cordyceps militaris peanut and preparing method thereof | |
| CN102584363B (en) | Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |