CN108676729B - Culture medium composition containing fermentation waste liquid, and preparation method and fermentation method thereof - Google Patents
Culture medium composition containing fermentation waste liquid, and preparation method and fermentation method thereof Download PDFInfo
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- CN108676729B CN108676729B CN201810520716.2A CN201810520716A CN108676729B CN 108676729 B CN108676729 B CN 108676729B CN 201810520716 A CN201810520716 A CN 201810520716A CN 108676729 B CN108676729 B CN 108676729B
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Abstract
The invention provides a culture medium composition containing fermentation waste liquid, a preparation method and a fermentation method thereof, belonging to the technical field of secondary fermentation and utilization of the fermentation waste liquid. The dry weight and iron content of the mycelium of the agaricus blazei murill obtained by performing liquid fermentation on the culture medium containing the fermentation waste liquid are obviously improved compared with the fermentation waste liquid, the fermentation waste liquid and the fermentation stock solution which are mixed and proportioned, and the agaricus blazei murill has obvious advantages in the aspects of cost, waste recycling, environmental friendliness and the like.
Description
Technical Field
The invention relates to the technical field of secondary fermentation and utilization of fermentation waste liquid, and particularly relates to a culture medium composition containing the fermentation waste liquid, and a preparation method and a fermentation method thereof.
Background
With the progress of human civilization and the continuous development of national economy, people pay more and more attention to their health.
Agaricus blazei murill is also called as Agaricus blazei Murill or Agaricus blazei Murill, is native to Brazil, Peru and the like, and belongs to edible and medicinal mushrooms. The agaricus blazei murill is extremely rich in nutrition, excellent in taste, pure, fresh and fragrant in taste and quite high in edible value. The protein composition comprises 18 amino acids, 8 essential amino acids of human body are complete, and also contains various vitamins and ergosterol. One of the treasures that people pay for the noble and Tianhuang in japan is called "mushroom-Zhenziyu".
The fruiting body of Agaricus blazei Murill contains various natural polysaccharide components, and can improve the living quality of specific people. The product was recognized by the U.S. food and drug administration as a "nutritional supplement for enhancing immunity" in 9 months 1999. It is because of such an attractive nutritional value and health care function that the consumption of agaricus blazei murill is raised in japan in recent years.
The fermentation waste liquid is harmful and nontoxic industrial waste water. Along with the gradual expansion of the application range of fermentation technology, the construction and development of the fermentation industry in China are faster and faster, so the treatment problem of the industrial wastewater is more and more emphasized by people. At present, relevant scientific research units in China already do a lot of work in the aspects of treatment and reutilization of wastewater in the fermentation industry, and the main methods comprise the steps of producing organic and inorganic fertilizers and poultry feed additives by using the wastewater, producing biological pesticides, producing biological flocculants, forming biomass hydrogen energy and the like. However, these methods produce little value. Therefore, a better way for recycling the fermentation waste liquid needs to be found, and it is urgent to fully utilize the fermentation waste liquid and generate higher income for enterprises.
Production enterprises generally treat production waste liquid as sewage, so that resources are wasted, the sewage treatment cost and the environmental pollution load of the enterprises are increased, and the development of the fermentation industry is restricted. Although manufacturing enterprises, research institutions and related colleges and universities have conducted a great deal of research on governance. However, no mature complete technology is applied to production practice at home and abroad at present. The main problems are that one-time investment is too large, or daily operation cost is too high, most manufacturers cannot bear the cost, and the current situation of standard exceeding emission has to be maintained for a long time.
With the development of industry and the expansion of production scale, the yield of fermentation waste liquid is continuously increased, and if the fermentation waste liquid cannot be effectively and properly treated, the economic and environmental burdens of enterprises are increased, and the healthy development of the enterprises is hindered.
If the fermentation waste liquid can be recycled for production, the environmental pollution can be reduced, and the waste utilization can be realized, thereby generating great economic and ecological benefits.
Therefore, the research on the environment-friendly process for treating the agaricus blazei murill fermentation waste liquid has important practical significance for reducing the wastewater pollution, reducing the sewage treatment burden, increasing the economic benefit of enterprises and improving the industrial added value to the maximum extent.
In view of the above, the present invention provides a culture medium composition containing fermentation waste liquid, a method for preparing the same, and a fermentation method.
Disclosure of Invention
The invention aims to provide a culture medium composition containing fermentation waste liquid, a preparation method and a fermentation method thereof.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
in a first aspect, the invention provides a culture medium composition containing fermentation waste liquid, which comprises the fermentation waste liquid, yeast and corn ultra-fine powder.
Further, the yeast in the culture medium composition containing the fermentation waste liquid is the iron-containing yeast after wall breaking.
Further, the fermentation waste liquid is waste liquid obtained after fermentation of the agaricus blazei murill mycelium liquid fermentation medium composition, and the agaricus blazei murill mycelium liquid fermentation stock solution is composed of 0.6 part of wall-broken iron-containing yeast, 20 parts of glucose, 0.3 part of monopotassium phosphate and 0.2 part of magnesium sulfate.
Further, the culture medium composition contains 0.06-0.18 g of wall-broken iron-containing yeast and 10-30 g of corn ultra-fine powder in each liter of fermentation waste liquid of agaricus blazei mycelium liquid fermentation waste liquid.
Further, the culture medium composition contains 0.09-0.15 g of wall-broken iron-containing yeast and 15-25 g of corn ultra-fine powder in each liter of fermentation waste liquid of agaricus blazei mycelium liquid fermentation waste liquid.
Further, in a preferred embodiment of the present invention, the culture medium composition comprises a liquid fermentation waste of Agaricus blazei Murill mycelia, wherein 0.12g of wall-broken iron-containing yeast and 20g of the corn ultra fine powder are added per liter of the fermentation waste.
The wall-broken yeast containing iron and other culture medium components with the iron content of 8000mg/kg are purchased from the market, the grain diameter of the corn ultra-micro powder is below 10 mu m, and the agaricus blazei murill seeds used for liquid fermentation are prepared according to the conventional method.
In a second aspect, the present invention provides a method for preparing the above culture medium composition containing fermentation waste liquid, comprising:
a preparation method of a culture medium containing agaricus blazei mycelium liquid fermentation waste liquid comprises the steps of adding a culture medium composition which contains 0.06-0.18 g of wall-broken iron-containing yeast and 10-30 g of corn ultra-fine powder into each liter of fermentation waste liquid, sterilizing at 121 ℃ for 15-30 min, and cooling to obtain the agaricus blazei mycelium liquid fermentation waste liquid.
In a third aspect, the present invention also provides a liquid fermentation method of Agaricus blazei Murill mycelia containing iron, which comprises: inoculating agaricus blazei murill seeds into the liquid culture medium containing the agaricus blazei murill mycelium liquid fermentation waste liquid, performing liquid fermentation at the temperature of 25-27 ℃, ventilating and stirring for 3-15 d, filtering or centrifuging the cultured fermentation liquid at 2000-10000 rpm, collecting mycelia, washing and drying to obtain the iron-containing agaricus blazei murill mycelium.
Further, in a preferred embodiment of the present invention, Agaricus blazei Murill seeds are inoculated into the liquid culture medium containing the liquid fermentation waste liquid of Agaricus blazei Murill mycelia, liquid fermentation is carried out at 26 ℃, aeration and agitation are carried out, the fermentation time is 7d, the cultured fermentation liquid is centrifuged at 4000rpm, mycelia are collected, and the mycelia containing iron are obtained after washing and drying.
Compared with the prior art, the invention has the beneficial effects that:
compared with the prior art, the dry weight and the iron content of the agaricus blazei murill mycelium are both obviously improved.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The features and properties of the present invention are described in further detail below with reference to examples:
example 1
0.06g of wall-broken iron-containing yeast and 30g of corn ultra-fine powder are added into 1L of agaricus blazei mycelium liquid fermentation waste liquid, sterilized at 121 ℃ for 30min and cooled.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 25 deg.C under stirring for 15d, centrifuging the cultured fermentation liquid at 2000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Example 2
Adding 0.18g of wall-broken iron-containing yeast and 10g of corn ultra-fine powder into 1L of Agaricus Blazei Murr mycelium liquid fermentation waste liquid, sterilizing at 121 deg.C for 15min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, performing liquid fermentation at 27 deg.C under stirring for 3d, centrifuging the cultured fermentation liquid at 10000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Example 3
Adding 0.09g of wall-broken iron-containing yeast and 25g of corn ultra-fine powder into 1L of Agaricus Blazei Murr mycelium liquid fermentation waste liquid, sterilizing at 121 deg.C for 20min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 25 deg.C for 5d under aeration and stirring, press-filtering the cultured fermentation liquid with 60kg pressure by plate-and-frame filter, collecting mycelium, washing, and drying to obtain Agaricus blazei Murill mycelium containing iron.
Example 4
Adding 0.15g of wall-broken iron-containing yeast and 15g of corn ultra-fine powder into 1L of Agaricus Blazei Murr mycelium liquid fermentation waste liquid, sterilizing at 121 deg.C for 20min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 26 deg.C under aeration and stirring for 7d, centrifuging the cultured fermentation liquid at 5000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Example 5
0.12g of wall-broken iron-containing yeast and 20g of corn ultra-fine powder are added into 1L of agaricus blazei mycelium liquid fermentation waste liquid, and the mixture is sterilized at 121 ℃ for 25min and then cooled.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 26 deg.C under aeration and stirring for 7d, centrifuging the cultured fermentation liquid at 4000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Comparative example 1
1L Agaricus blazei Murill mycelium liquid fermentation stock solution, sterilizing at 121 deg.C for 25min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 26 deg.C under aeration and stirring for 7d, centrifuging the cultured fermentation liquid at 4000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Comparative example 2
Sterilizing 1L waste liquid of Agaricus blazei mycelium liquid fermentation at 121 deg.C for 25min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 26 deg.C under aeration and stirring for 7d, centrifuging the cultured fermentation liquid at 4000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Comparative example 3
Taking 1L mixed culture medium composed of Agaricus blazei Murill mycelium liquid fermentation waste liquid and Agaricus blazei Murill mycelium liquid fermentation stock solution (waste liquid: stock solution: 1), sterilizing at 121 deg.C for 25min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 26 deg.C under aeration and stirring for 7d, centrifuging the cultured fermentation liquid at 4000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Comparative example 4
Taking 1L mixed culture medium composed of Agaricus blazei Murill mycelium liquid fermentation waste liquid and Agaricus blazei Murill mycelium liquid fermentation stock solution (waste liquid: stock solution: 1: 2), sterilizing at 121 deg.C for 25min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 26 deg.C under aeration and stirring for 7d, centrifuging the cultured fermentation liquid at 4000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
Comparative example 5
Taking 1L mixed culture medium composed of Agaricus blazei Murill mycelium liquid fermentation waste liquid and Agaricus blazei Murill mycelium liquid fermentation stock solution (waste liquid: stock solution: 1: 3), sterilizing at 121 deg.C for 25min, and cooling.
Inoculating Agaricus blazei Murill seed into the liquid culture medium, fermenting at 26 deg.C under aeration and stirring for 7d, centrifuging the cultured fermentation liquid at 4000rpm, collecting mycelium, washing, and drying to obtain iron-containing Agaricus blazei Murill mycelium.
The beneficial effects of the invention are as follows:
from the above table, it can be seen that the dry weight and iron content of the mycelium of the agaricus blazei murill obtained by performing liquid fermentation on the culture medium containing the fermentation waste liquid according to the present invention in each group of embodiments of the present invention are significantly improved compared with the fermentation waste liquid and the mixture ratio of the fermentation waste liquid and the fermentation stock solution, and the present invention has obvious advantages in aspects of cost, waste material utilization, environmental friendliness, etc.
The details which are not described in the present invention are the common general knowledge which can be selected by the ordinary skilled person in the art. Although the invention has been described in detail hereinabove with respect to specific embodiments thereof, it will be apparent to those skilled in the art that modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (3)
1. A culture medium composition containing fermentation waste liquid is characterized in that the culture medium composition containing the fermentation waste liquid comprises the fermentation waste liquid, corn superfine powder and wall-broken iron-containing yeast; the fermentation waste liquid is waste liquid obtained after fermentation of the agaricus blazei mycelium liquid fermentation medium composition, and 0.06-0.18 g of wall-broken iron-containing yeast and 10-30 g of corn ultra-fine powder are added into each liter of fermentation waste liquid.
2. The culture medium composition containing the fermentation waste liquid as claimed in claim 1, wherein the amount of the iron-containing yeast after wall breaking is 0.09-0.15 g, and the amount of the corn ultra-fine powder is 15-25 g.
3. The culture medium composition containing the fermentation waste liquid according to claim 1, wherein the amount of the iron-containing yeast after wall breaking is 0.12g, and the amount of the corn ultra-micro powder is 20 g.
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