CN105361162A - Making method of cordyceps militaris Hami melon enzyme capable of retarding senile dementia - Google Patents

Making method of cordyceps militaris Hami melon enzyme capable of retarding senile dementia Download PDF

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Publication number
CN105361162A
CN105361162A CN201510865310.4A CN201510865310A CN105361162A CN 105361162 A CN105361162 A CN 105361162A CN 201510865310 A CN201510865310 A CN 201510865310A CN 105361162 A CN105361162 A CN 105361162A
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cordyceps militaris
liquid
enzyme
ferment
minute
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不公告发明人
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Shenyang Yuankang Natural Biological Technology Co Ltd
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Shenyang Yuankang Natural Biological Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2220/00Lactobacillus

Abstract

The invention relates to a making method of a cordyceps militaris Hami melon enzyme capable of retarding senile dementia. The making method comprises the following steps of step 1, preparing a microzyme strain solution; step 2, preparing a lactic acid bacteria strain solution; step 3, performing fermentation culture; step 4, performing centrifugation treatment so as to obtain an enzyme stock solution; and step 5, adding a cordycepin extract to the enzyme stock solution. The making method disclosed by the invention has the beneficial effects that respective characteristics of an enzyme and respective characteristics of cordyceps militaris are combined, the enzyme and the cordyceps militaris are in mixed fermentation or compounding, and then the enzyme and the cordyceps militaris after mixed fermentation or compounding is performed are matched with other active components, so that a cordyceps militaris enzyme product is innovatively developed; the cordyceps militaris enzyme product integrates the efficacies of the enzyme and the efficacies of the cordyceps militaris, and has the efficacies of retarding the senile dementia, reducing body weight, strengthening bodies and prolonging life.

Description

A kind of preparation method slowing down the Cordyceps militaris "Hami" melon ferment of senile dementia
Technical field
The invention belongs to biologic product technology field, particularly a kind of preparation method slowing down the Cordyceps militaris "Hami" melon ferment of senile dementia.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Modern medicine study its there is cellular immunity and Humoral Immunological Regulation Roles, also there is antifatigue effect.Therefore, be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.Further, existing ferment slows down senile dementia weak effect.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of preparation method slowing down the Cordyceps militaris "Hami" melon ferment of senile dementia.
Technical scheme of the present invention is achieved in that the method comprises the steps:
Step 1: prepare barms liquid
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2. prepares lactobacillus inoculation liquid
Plant flow container and expand culture medium: enrichment medium of wort, its 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4,all the other are water, and enrichment medium of wort is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Be dissolved in the sterilized water of 3 times by 1.5 kilograms of lactic acid bacteria dry bacterium powders under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3. fermented and cultured
Be that the "Hami" melon of 2:10 and water mixing are smashed rear filtration and obtained filtrate by mass ratio, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4. centrifugal treating, obtains ferment stoste
Zymotic fluid imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5, add cordycepin extractive to ferment stoste, be 1000:10 according to ferment stoste and cordycepin extractive mass ratio, high-temperature sterilization 30-50 minute at 115 DEG C, carries out packing.
Described lactic acid bacteria is Bifidobacterium, and its quality is 1.5 kilograms.
Beneficial effect of the present invention: both, in conjunction with the features of ferment and Cordyceps militaris, are carried out mixed culture fermentation or composite, then be equipped with other active component, develop Cordyceps militaris ferment product innovatively by the present invention.Effect of this product set ferment and Cordyceps militaris, has and slows down senile dementia, improve the health, the effect prolonged life.
Detailed description of the invention
Slow down a preparation method for the Cordyceps militaris "Hami" melon ferment of senile dementia, comprise the steps:
Step 1: prepare barms liquid
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2. prepares lactobacillus inoculation liquid
Plant flow container and expand culture medium: enrichment medium of wort, its 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4,all the other are water, and enrichment medium of wort is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Be dissolved in the sterilized water of 3 times by 1.5 kilograms of lactic acid bacteria dry bacterium powders under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3. fermented and cultured
Be that the "Hami" melon of 2:10 and water mixing are smashed rear filtration and obtained filtrate by mass ratio, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid.
Step 4. centrifugal treating, obtains ferment stoste
Zymotic fluid imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5, add cordycepin extractive to ferment stoste, be 1000:10 according to ferment stoste and cordycepin extractive mass ratio, high-temperature sterilization 30-50 minute at 115 DEG C, carries out packing.
Described lactic acid bacteria is Bifidobacterium, and its quality is 1.5 kilograms.
The present invention delays senile dementia and obtains effect by experiment:
The senile dementia patient that participation Cordyceps militaris ferment is taken has 35 people, and sooner or later respectively taking 1 time, each 200ml for each person every day, 90 days is an evaluation and test cycle.After using ferment of the present invention, efficiently individual quantity 25 people, all have in various degree delay effect, symptom is developing deeply not.Wherein 9 people, have certain recovery, and maintain better state, remain unchanged.

Claims (2)

1. slow down a preparation method for the Cordyceps militaris "Hami" melon ferment of senile dementia, it is characterized in that: comprise the steps:
Step 1: prepare barms liquid
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water, and YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2. prepares lactobacillus inoculation liquid
Plant flow container and expand culture medium: enrichment medium of wort, its 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4,all the other are water, and enrichment medium of wort is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Be dissolved in the sterilized water of 3 times by 1.5 kilograms of lactic acid bacteria dry bacterium powders under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3. fermented and cultured
Be that the "Hami" melon of 2:10 and water mixing are smashed rear filtration and obtained filtrate by mass ratio, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4. centrifugal treating, obtains ferment stoste
Zymotic fluid imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5, add cordycepin extractive to ferment stoste, be 1000:10 according to ferment stoste and cordycepin extractive mass ratio, high-temperature sterilization 30-50 minute at 115 DEG C, carries out packing.
2. the preparation method slowing down the Cordyceps militaris "Hami" melon ferment of senile dementia according to claim 1, it is characterized in that: described lactic acid bacteria is Bifidobacterium, its quality is 1.5 kilograms.
CN201510865310.4A 2015-12-01 2015-12-01 Making method of cordyceps militaris Hami melon enzyme capable of retarding senile dementia Pending CN105361162A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105919076A (en) * 2016-04-26 2016-09-07 青岛金凤凰庄园果品有限公司 Fermentation method of blackberry single ferment
CN108244429A (en) * 2018-01-09 2018-07-06 安徽妙奇树生物科技有限公司 A kind of preparation method of "Hami" melon composite enzyme drink

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140057838A (en) * 2012-11-05 2014-05-14 노길형 Fermented foods process and production method
CN103960613A (en) * 2014-05-09 2014-08-06 亳州千草药业有限公司 Method for preparing fruit and vegetable enzymes
CN104738780A (en) * 2015-04-21 2015-07-01 杭州曜拓贸易有限公司 Integrated fruit enzyme and preparation method thereof
CN104856015A (en) * 2015-04-24 2015-08-26 西藏月王生物技术有限公司 A cordyceps sinensis enzyme and a preparation method thereof
CN104886572A (en) * 2015-06-02 2015-09-09 广州加海生物科技有限公司 A fruit and vegetable ferment beverage and a preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140057838A (en) * 2012-11-05 2014-05-14 노길형 Fermented foods process and production method
CN103960613A (en) * 2014-05-09 2014-08-06 亳州千草药业有限公司 Method for preparing fruit and vegetable enzymes
CN104738780A (en) * 2015-04-21 2015-07-01 杭州曜拓贸易有限公司 Integrated fruit enzyme and preparation method thereof
CN104856015A (en) * 2015-04-24 2015-08-26 西藏月王生物技术有限公司 A cordyceps sinensis enzyme and a preparation method thereof
CN104886572A (en) * 2015-06-02 2015-09-09 广州加海生物科技有限公司 A fruit and vegetable ferment beverage and a preparation method thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
佟林峰等: "蛹虫草多糖配合果蔬酵素对抗氧化作用的研究", 《安徽农业科学》 *
李艳: "《发酵工业概论》", 31 July 2011, 中国轻工业出版社 *
沙莎等: "《动物微生物实验教程》", 31 August 2011, 西南师范大学出版社 *
陈士怡等: "《酵母遗传学》", 31 October 1989, 科学出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105919076A (en) * 2016-04-26 2016-09-07 青岛金凤凰庄园果品有限公司 Fermentation method of blackberry single ferment
CN108244429A (en) * 2018-01-09 2018-07-06 安徽妙奇树生物科技有限公司 A kind of preparation method of "Hami" melon composite enzyme drink

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