CN105433385A - Preparation method of immunity-improving cordyceps militaris and tomato enzyme - Google Patents
Preparation method of immunity-improving cordyceps militaris and tomato enzyme Download PDFInfo
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- CN105433385A CN105433385A CN201510865198.4A CN201510865198A CN105433385A CN 105433385 A CN105433385 A CN 105433385A CN 201510865198 A CN201510865198 A CN 201510865198A CN 105433385 A CN105433385 A CN 105433385A
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 10
- 102000004190 Enzymes Human genes 0.000 title abstract description 10
- 108090000790 Enzymes Proteins 0.000 title abstract description 10
- 240000003768 Solanum lycopersicum Species 0.000 title abstract 2
- 239000007788 liquid Substances 0.000 claims abstract description 32
- 241000894006 Bacteria Species 0.000 claims abstract description 25
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 230000036039 immunity Effects 0.000 claims abstract description 11
- 239000000284 extract Substances 0.000 claims abstract description 10
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 10
- 239000004310 lactic acid Substances 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 12
- 238000010899 nucleation Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 241000233866 Fungi Species 0.000 claims description 9
- 240000001307 Myosotis scorpioides Species 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 241000227653 Lycopersicon Species 0.000 claims description 8
- 241000186000 Bifidobacterium Species 0.000 claims description 6
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 6
- 241000186660 Lactobacillus Species 0.000 claims description 6
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 6
- 241001052560 Thallis Species 0.000 claims description 6
- 229960005305 adenosine Drugs 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 229940039696 lactobacillus Drugs 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 241000235342 Saccharomycetes Species 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 230000008676 import Effects 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 108060003951 Immunoglobulin Proteins 0.000 abstract description 2
- 102000018358 immunoglobulin Human genes 0.000 abstract description 2
- 239000011550 stock solution Substances 0.000 abstract 2
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000013329 compounding Methods 0.000 abstract 1
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 abstract 1
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 abstract 1
- 230000002708 enhancing effect Effects 0.000 abstract 1
- 239000000047 product Substances 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 210000000952 spleen Anatomy 0.000 description 5
- 210000001541 thymus gland Anatomy 0.000 description 4
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 101100256850 Drosophila melanogaster EndoA gene Proteins 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 206010039966 Senile dementia Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000004279 orbit Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002992 thymic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A preparation method of an immunity-improving cordyceps militaris and tomato enzyme comprises the following steps: step 1, preparation of a yeast seed liquid; step 2, preparation of a lactic acid bacteria seed liquid; step 3, fermentation culture; step 4, centrifugal treatment to obtain an enzyme stock solution; step 5, addition of a cordycepin extract into the enzyme stock solution. The preparation method has the advantages that the characteristics of enzyme and cordyceps militaris are combined, enzyme and cordyceps militaris are mixed for fermentation or compounding, and other active ingredients are added, so as to develop the cordyceps militaris enzyme product innovatively. The product integrates the efficacies of enzyme and cordyceps militaris into a whole, thereby having the efficacies of improving immunity, enhancing immune globulin, and building bodies.
Description
Technical field
The invention belongs to biologic product technology field, particularly a kind of preparation method improving the Cordyceps militaris tomato ferment of immunity.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Modern medicine study its there is cellular immunity and Humoral Immunological Regulation Roles, also there is antifatigue effect.Therefore, be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.Further, existing ferment raising immunity aspect effect is poor.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of preparation method improving the Cordyceps militaris tomato ferment of immunity.
Technical scheme of the present invention is achieved in that the method comprises the steps:
Step 1: prepare barms liquid
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, and 2% apple sugar, 2% agar, all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2. prepares lactobacillus inoculation liquid
Plant flow container and expand culture medium: enrichment medium of wort, its 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Be dissolved in the sterilized water of 3 times by 1.5 kilograms of lactic acid bacteria dry bacterium powders under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3. fermented and cultured
Be the tomato of 1.5:0.5:10 by mass ratio, apple and water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4. centrifugal treating, obtains ferment stoste
Zymotic fluid imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5, adding Chinese caterpillar fungus adenosine extract to ferment stoste, according to ferment stoste and Chinese caterpillar fungus adenosine extract quality than being 1000:50, high-temperature sterilization 30-50 minute at 115 DEG C, carrying out packing.
Described lactic acid bacteria is streptococcus thermophilus and Bifidobacterium, and wherein streptococcus thermophilus quality is 1 kilogram, and Bifidobacterium quality is 0.5 kilogram.
Beneficial effect of the present invention: both, in conjunction with the features of ferment and Cordyceps militaris, are carried out mixed culture fermentation or composite, then be equipped with other active component, develop Cordyceps militaris ferment product innovatively by the present invention.Effect of this product set ferment and Cordyceps militaris, has raising immunity, strengthens immunoglobulin (Ig), the effect improved the health.
Detailed description of the invention
Slow down a preparation method for the Cordyceps militaris apple ferment of senile dementia, comprise the steps:
Step 1: prepare barms liquid
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, and 2% apple sugar, 2% agar, all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2. prepares lactobacillus inoculation liquid
Plant flow container and expand culture medium: enrichment medium of wort, its 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Be dissolved in the sterilized water of 3 times by 1.5 kilograms of lactic acid bacteria dry bacterium powders under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3. fermented and cultured
Be the tomato of 1.5:0.5:10 by mass ratio, apple and water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4. centrifugal treating, obtains ferment stoste
Zymotic fluid imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5, adding Chinese caterpillar fungus adenosine extract to ferment stoste, according to ferment stoste and Chinese caterpillar fungus adenosine extract quality than being 1000:50, high-temperature sterilization 30-50 minute at 115 DEG C, carrying out packing.
Described lactic acid bacteria is streptococcus thermophilus and Bifidobacterium, and wherein streptococcus thermophilus quality is 1 kilogram, and Bifidobacterium quality is 0.5 kilogram.
The present invention improves immunity and obtains effect by experiment:
Adopt feeding method to feed kunming mice, be divided at random: Normal group (pure water group), embodiment product group (Chinese caterpillar fungus ferment group), often organize 10.Chinese caterpillar fungus ferment group gives Cordyceps militaris ferment tested material with the volume of 0.1mL/20g every day, and negative control group gives equal-volume pure water, after continuous 30d, weigh, eye socket is got blood and is put to death, and divides and gets thymus gland and spleen, weigh, calculate thymus index and index and spleen index respectively by following formula:
Thymus index=thymic weight (mg)/mice weights (g)
Index and spleen index=spleen weight (mg)/mice weights (g)
The results are shown in Table, Chinese caterpillar fungus ferment group Thymus and Spleen index is all higher than Normal group, and difference has conspicuousness (p < 0.01), illustrates that the immunity of product group promotes Be very effective.
。
Claims (2)
1. improve a preparation method for the Cordyceps militaris tomato ferment of immunity, it is characterized in that: comprise the steps:
Step 1: prepare barms liquid
Plant flow container and expand culture medium: YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, and 2% apple sugar, 2% agar, all the other are water, and YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, passes into aseptic compressed air at 28-30 DEG C, and throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2. prepares lactobacillus inoculation liquid
Plant flow container and expand culture medium: enrichment medium of wort, its 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,all the other are water, and enrichment medium of wort is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C;
Be dissolved in the sterilized water of 3 times by 1.5 kilograms of lactic acid bacteria dry bacterium powders under aseptic technique, bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, can repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3. fermented and cultured
Be the tomato of 1.5:0.5:10 by mass ratio, apple and water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4. centrifugal treating, obtains ferment stoste
Zymotic fluid imports centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5, adding Chinese caterpillar fungus adenosine extract to ferment stoste, according to ferment stoste and Chinese caterpillar fungus adenosine extract quality than being 1000:50, high-temperature sterilization 30-50 minute at 115 DEG C, carrying out packing.
2. the preparation method of the Cordyceps militaris tomato ferment of raising immunity according to claim 1, it is characterized in that: described lactic acid bacteria is streptococcus thermophilus and Bifidobacterium, wherein streptococcus thermophilus quality is 1 kilogram, and Bifidobacterium quality is 0.5 kilogram.
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| CN201510865198.4A CN105433385A (en) | 2015-12-01 | 2015-12-01 | Preparation method of immunity-improving cordyceps militaris and tomato enzyme |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106962927A (en) * | 2017-04-28 | 2017-07-21 | 邓和满 | The preparation method of Cordyceps militaris ferment |
| CN110692882A (en) * | 2019-11-06 | 2020-01-17 | 山东博华高效生态农业科技有限公司 | Tomato enzyme beverage with eucommia flavor and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962927A (en) * | 2017-04-28 | 2017-07-21 | 邓和满 | The preparation method of Cordyceps militaris ferment |
| CN110692882A (en) * | 2019-11-06 | 2020-01-17 | 山东博华高效生态农业科技有限公司 | Tomato enzyme beverage with eucommia flavor and preparation method thereof |
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Application publication date: 20160330 |