CN105495573A - Preparation process of cordyceps militaris and bitter orange enzyme with weight loss effect - Google Patents
Preparation process of cordyceps militaris and bitter orange enzyme with weight loss effect Download PDFInfo
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 31
- 230000000694 effects Effects 0.000 title claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 title abstract description 11
- 102000004190 Enzymes Human genes 0.000 title abstract description 11
- 238000002360 preparation method Methods 0.000 title abstract 3
- 244000183685 Citrus aurantium Species 0.000 title abstract 2
- 235000007716 Citrus aurantium Nutrition 0.000 title abstract 2
- 230000004580 weight loss Effects 0.000 title abstract 2
- 238000000855 fermentation Methods 0.000 claims abstract description 18
- 230000004151 fermentation Effects 0.000 claims abstract description 18
- 239000000284 extract Substances 0.000 claims abstract description 17
- 241000186660 Lactobacillus Species 0.000 claims abstract description 11
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 11
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims abstract description 7
- 229940002508 ginger extract Drugs 0.000 claims abstract description 7
- 235000020708 ginger extract Nutrition 0.000 claims abstract description 7
- 235000010241 potassium sorbate Nutrition 0.000 claims abstract description 7
- 239000004302 potassium sorbate Substances 0.000 claims abstract description 7
- 229940069338 potassium sorbate Drugs 0.000 claims abstract description 7
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 55
- 241000894006 Bacteria Species 0.000 claims description 47
- 239000002609 medium Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- 239000000706 filtrate Substances 0.000 claims description 20
- 239000012530 fluid Substances 0.000 claims description 20
- 239000001963 growth medium Substances 0.000 claims description 20
- 238000011081 inoculation Methods 0.000 claims description 20
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 20
- 238000010899 nucleation Methods 0.000 claims description 20
- 230000001954 sterilising effect Effects 0.000 claims description 20
- 238000004659 sterilization and disinfection Methods 0.000 claims description 20
- 239000000843 powder Substances 0.000 claims description 17
- 238000000034 method Methods 0.000 claims description 15
- 241001052560 Thallis Species 0.000 claims description 10
- 229940041514 candida albicans extract Drugs 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 10
- 235000014655 lactic acid Nutrition 0.000 claims description 10
- 239000004310 lactic acid Substances 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 210000002966 serum Anatomy 0.000 claims description 10
- 239000012138 yeast extract Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- 241000186000 Bifidobacterium Species 0.000 claims description 7
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 7
- 241000235342 Saccharomycetes Species 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 5
- 235000005979 Citrus limon Nutrition 0.000 claims description 5
- 244000131522 Citrus pyriformis Species 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 235000014360 Punica granatum Nutrition 0.000 claims description 5
- 239000008272 agar Substances 0.000 claims description 5
- 235000015278 beef Nutrition 0.000 claims description 5
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000009630 liquid culture Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000012856 packing Methods 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 229910052700 potassium Inorganic materials 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 244000294611 Punica granatum Species 0.000 claims 1
- 239000011550 stock solution Substances 0.000 abstract 7
- 235000019218 bitter orange extract Nutrition 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 239000000047 product Substances 0.000 description 9
- 230000008569 process Effects 0.000 description 5
- 241000219991 Lythraceae Species 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 238000011169 microbiological contamination Methods 0.000 description 4
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 235000012055 fruits and vegetables Nutrition 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 101100256850 Drosophila melanogaster EndoA gene Proteins 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000220225 Malus Species 0.000 description 1
- 235000011430 Malus pumila Nutrition 0.000 description 1
- 235000015103 Malus silvestris Nutrition 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000037221 weight management Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
Abstract
The invention provides a technical scheme about a preparation process of a cordyceps militaris and bitter orange enzyme with a weight loss effect. The preparation process comprises the following steps step 1 a yeast strain solution is prepared; step 2 a lactobacillus strain solution is prepared; step 3 fermentation culture is performed; step 4 an enzyme stock solution is obtained through centrifugal treatment; step 5 bitter orange extract, synanthrin, ginger extract, potassium sorbate and cordyceps militaris extract are added to the enzyme stock solution, the mass ratio of the bitter orange extract to the enzyme stock solution is 1100, the mass ratio of the synanthrin to the enzyme stock solution is 120, the mass ratio of the ginger extract to the enzyme stock solution is 1100, the mass ratio of potassium sorbate to the enzyme stock solution is 12,000, the mass ratio of the cordyceps militaris extract to the enzyme stock solution is 110, and the mixture is uniformly stirred and sterilized at the high temperature of 115 DEG C for 30-50 minutes and then sub-packaged.
Description
Technical field
The invention belongs to bio-science field, be specifically related to a kind of Cordyceps militaris bigarabe ferment manufacturing process having fat-reducing effect.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.Present fat phenomenon is more and more subject to people's attention, and various diet products emerge in an endless stream, but a lot of diet products can produce illeffects to health.Fruit and vegetable ferment is for dominant fermentation raw material with apple, tomato, Chinese cabbage, two benches respectively through the bacterial classification such as saccharomycete and lactic acid bacteria ferments, tunning removes dregs through centrifugation again, final acquisition fruit and vegetable ferment, ferment has very large benefit to the metabolism of human body and fat reducing, and therefore developing the Cordyceps militaris pectase with fat-reducing effect in conjunction with Cordyceps militaris has very large benefit to the mankind.
Summary of the invention
The invention provides a kind of Cordyceps militaris bigarabe ferment manufacturing process having fat-reducing effect, can play a positive role to the Weight management of people.
The technical solution used in the present invention is:
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3in seeding tank, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4, all the other are water; Enrichment medium of wort is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then being the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continuing to cultivate 5-7d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allowing tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
In described step 2, lactic acid bacteria is Bifidobacterium and streptococcus thermophilus, wherein Bifidobacterium dry bacterium powder 1 kilogram, streptococcus thermophilus dry bacterium powder 0.5 kilogram.
Compared with prior art, the invention has the beneficial effects as follows: the present invention is in conjunction with the features of ferment and Cordyceps militaris, both are carried out mixed culture fermentation or composite, be equipped with other active component again, develop Cordyceps militaris ferment product innovatively, effect of this product set ferment and Cordyceps militaris, has the effect optimized metabolic process, improve immunity and weight reducing fat reducing.
Detailed description of the invention
Embodiment one
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 30 minutes at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3in seeding tank, at 28 DEG C, pass into aseptic compressed air, throughput is 0.2 cubic meters per minute, cultivates 20h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4, all the other are water; Enrichment medium of wort is put into 0.5M3 seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1 kilogram of Bifidobacterium dry bacterium powder and 0.5 kilogram of streptococcus thermophilus dry bacterium powder are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid being loaded serum bottle and is inoculated into 0.5M3 seeding tank, 37 DEG C of constant temperature quiescent culture 24h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeating the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 5% of described filtrate by quality obtains, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is 5 cubic metres, throughput is 20 cubic meters per minute, speed of agitator 100-120rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 5% of described filtrate by quality obtains, continues to cultivate 5d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allows tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
Embodiment two
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 50 minutes at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3in seeding tank, at 30 DEG C, pass into aseptic compressed air, throughput is 0.25 cubic meters per minute, cultivates 30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4, all the other are water; Enrichment medium of wort is put into 0.5M
3in seeding tank, high-temperature sterilization 50 minutes at 115 DEG C; Under aseptic technique, 1 kilogram of Bifidobacterium dry bacterium powder and 0.5 kilogram of streptococcus thermophilus dry bacterium powder are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 7% of described filtrate by quality obtains, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is 5 cubic metres, throughput is 2.5 cubic meters per minute, speed of agitator 120rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 8% of described filtrate by quality obtains, continues to cultivate 7d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allows tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
Embodiment three
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 40 minutes at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3in seeding tank, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.25 cubic meters per minute, cultivates 40h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4, all the other are water; Enrichment medium of wort is put into 0.5M
3in seeding tank, high-temperature sterilization 40 minutes at 115 DEG C; Under aseptic technique, 1 kilogram of Bifidobacterium dry bacterium powder and 0.5 kilogram of streptococcus thermophilus dry bacterium powder are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 45 minutes at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 10% of described filtrate by quality obtains, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is 5 cubic metres, throughput is 3 cubic meters per minute, speed of agitator 110rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 10% of described filtrate by quality obtains, continues to cultivate 6d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allows tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
The evaluation and test volunteer that participation Cordyceps militaris ferment takes fat-reducing effect has 20 people, and sooner or later respectively taking 1 time, each 150ml for each person every day, 45 days is an evaluation and test cycle.The average weight of 20 people before evaluation and test is 70.2Kg, and at an all after date of evaluation and test, average weight is 67.5, decreases 2.7Kg, the Be very effective that loses weight of this ferment product.
Claims (2)
1. there is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, it is characterized in that comprising the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3in seeding tank, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4, all the other are water; Enrichment medium of wort is put into 0.5M
3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality;
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, centrifugal 10min under rotating speed is 6000rpm, and gained supernatant is ferment stoste;
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
2. a kind of Cordyceps militaris bigarabe ferment manufacturing process having fat-reducing effect according to claim 1, it is characterized in that, in described step 2, lactic acid bacteria is Bifidobacterium and streptococcus thermophilus, wherein Bifidobacterium dry bacterium powder 1 kilogram, streptococcus thermophilus dry bacterium powder 0.5 kilogram.
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