CN105495573A - Preparation process of cordyceps militaris and bitter orange enzyme with weight loss effect - Google Patents

Preparation process of cordyceps militaris and bitter orange enzyme with weight loss effect Download PDF

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Publication number
CN105495573A
CN105495573A CN201510865283.0A CN201510865283A CN105495573A CN 105495573 A CN105495573 A CN 105495573A CN 201510865283 A CN201510865283 A CN 201510865283A CN 105495573 A CN105495573 A CN 105495573A
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liquid
mass ratio
ferment
extract
cordyceps militaris
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不公告发明人
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Shenyang Yuankang Natural Biological Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2240/00Streptococcus, lactococcus
    • A23Y2240/75Thermophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23YINDEXING SCHEME RELATING TO LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23Y2300/00Bifidobacterium

Abstract

The invention provides a technical scheme about a preparation process of a cordyceps militaris and bitter orange enzyme with a weight loss effect. The preparation process comprises the following steps step 1 a yeast strain solution is prepared; step 2 a lactobacillus strain solution is prepared; step 3 fermentation culture is performed; step 4 an enzyme stock solution is obtained through centrifugal treatment; step 5 bitter orange extract, synanthrin, ginger extract, potassium sorbate and cordyceps militaris extract are added to the enzyme stock solution, the mass ratio of the bitter orange extract to the enzyme stock solution is 1100, the mass ratio of the synanthrin to the enzyme stock solution is 120, the mass ratio of the ginger extract to the enzyme stock solution is 1100, the mass ratio of potassium sorbate to the enzyme stock solution is 12,000, the mass ratio of the cordyceps militaris extract to the enzyme stock solution is 110, and the mixture is uniformly stirred and sterilized at the high temperature of 115 DEG C for 30-50 minutes and then sub-packaged.

Description

A kind of Cordyceps militaris bigarabe ferment manufacturing process having fat-reducing effect
Technical field
The invention belongs to bio-science field, be specifically related to a kind of Cordyceps militaris bigarabe ferment manufacturing process having fat-reducing effect.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.Present fat phenomenon is more and more subject to people's attention, and various diet products emerge in an endless stream, but a lot of diet products can produce illeffects to health.Fruit and vegetable ferment is for dominant fermentation raw material with apple, tomato, Chinese cabbage, two benches respectively through the bacterial classification such as saccharomycete and lactic acid bacteria ferments, tunning removes dregs through centrifugation again, final acquisition fruit and vegetable ferment, ferment has very large benefit to the metabolism of human body and fat reducing, and therefore developing the Cordyceps militaris pectase with fat-reducing effect in conjunction with Cordyceps militaris has very large benefit to the mankind.
Summary of the invention
The invention provides a kind of Cordyceps militaris bigarabe ferment manufacturing process having fat-reducing effect, can play a positive role to the Weight management of people.
The technical solution used in the present invention is:
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3in seeding tank, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4, all the other are water; Enrichment medium of wort is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then being the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continuing to cultivate 5-7d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allowing tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
In described step 2, lactic acid bacteria is Bifidobacterium and streptococcus thermophilus, wherein Bifidobacterium dry bacterium powder 1 kilogram, streptococcus thermophilus dry bacterium powder 0.5 kilogram.
Compared with prior art, the invention has the beneficial effects as follows: the present invention is in conjunction with the features of ferment and Cordyceps militaris, both are carried out mixed culture fermentation or composite, be equipped with other active component again, develop Cordyceps militaris ferment product innovatively, effect of this product set ferment and Cordyceps militaris, has the effect optimized metabolic process, improve immunity and weight reducing fat reducing.
Detailed description of the invention
Embodiment one
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 30 minutes at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3in seeding tank, at 28 DEG C, pass into aseptic compressed air, throughput is 0.2 cubic meters per minute, cultivates 20h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4, all the other are water; Enrichment medium of wort is put into 0.5M3 seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1 kilogram of Bifidobacterium dry bacterium powder and 0.5 kilogram of streptococcus thermophilus dry bacterium powder are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid being loaded serum bottle and is inoculated into 0.5M3 seeding tank, 37 DEG C of constant temperature quiescent culture 24h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeating the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 5% of described filtrate by quality obtains, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is 5 cubic metres, throughput is 20 cubic meters per minute, speed of agitator 100-120rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 5% of described filtrate by quality obtains, continues to cultivate 5d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allows tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
Embodiment two
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 50 minutes at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3in seeding tank, at 30 DEG C, pass into aseptic compressed air, throughput is 0.25 cubic meters per minute, cultivates 30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4, all the other are water; Enrichment medium of wort is put into 0.5M 3in seeding tank, high-temperature sterilization 50 minutes at 115 DEG C; Under aseptic technique, 1 kilogram of Bifidobacterium dry bacterium powder and 0.5 kilogram of streptococcus thermophilus dry bacterium powder are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 7% of described filtrate by quality obtains, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is 5 cubic metres, throughput is 2.5 cubic meters per minute, speed of agitator 120rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 8% of described filtrate by quality obtains, continues to cultivate 7d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allows tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
Embodiment three
There is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, comprise the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 40 minutes at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3in seeding tank, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.25 cubic meters per minute, cultivates 40h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4, all the other are water; Enrichment medium of wort is put into 0.5M 3in seeding tank, high-temperature sterilization 40 minutes at 115 DEG C; Under aseptic technique, 1 kilogram of Bifidobacterium dry bacterium powder and 0.5 kilogram of streptococcus thermophilus dry bacterium powder are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality.
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 45 minutes at 115 DEG C, described culture medium is added in fermentation tank, then the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 being 10% of described filtrate by quality obtains, at the temperature of 28 DEG C, cultivate 48h, the volume of described fermentation tank is 5 cubic metres, throughput is 3 cubic meters per minute, speed of agitator 110rpm/min; Then the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 being 10% of described filtrate by quality obtains, continues to cultivate 6d, this process quiescent culture at the temperature of 37 DEG C, without the need to stirring, allows tank keep malleation in case microbiological contamination, until fermentation ends obtains zymotic fluid.
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, and centrifugal 10min under rotating speed is 6000rpm, gained supernatant is ferment stoste.
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
The evaluation and test volunteer that participation Cordyceps militaris ferment takes fat-reducing effect has 20 people, and sooner or later respectively taking 1 time, each 150ml for each person every day, 45 days is an evaluation and test cycle.The average weight of 20 people before evaluation and test is 70.2Kg, and at an all after date of evaluation and test, average weight is 67.5, decreases 2.7Kg, the Be very effective that loses weight of this ferment product.

Claims (2)

1. there is a Cordyceps militaris bigarabe ferment manufacturing process for fat-reducing effect, it is characterized in that comprising the following steps:
Step 1: prepared by barms liquid;
Select YPD fluid nutrient medium for kind of a flow container expansion culture medium, the mass percent proportioning of YPD Liquid Culture based component is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and all the other are water; YPD fluid nutrient medium is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3in seeding tank, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3 cubic meters per minute, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepared by lactobacillus inoculation liquid;
Select enrichment medium of wort to be that kind of flow container expands culture medium, the mass percent proportioning of enrichment medium of wort composition is 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K 2hPO 4, all the other are water; Enrichment medium of wort is put into 0.5M 3in seeding tank, high-temperature sterilization 30-50 minute at 115 DEG C; Under aseptic technique, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 4.5 kilograms, form bacterium liquid; Described bacterium liquid is loaded serum bottle and is inoculated into 0.5M 3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain the lactic acid bacteria of required quality;
Step 3: fermented and cultured;
Be the orange of 130:20:20:1000 by mass ratio, lemon, pomegranate, water mixing smash rear filtration and obtain filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator 100-120rpm/min; Then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid;
Step 4: centrifugal treating obtains ferment stoste;
Zymotic fluid obtained for step 3 is imported centrifuge, centrifugal 10min under rotating speed is 6000rpm, and gained supernatant is ferment stoste;
Step 5: to ferment stoste add with ferment stoste mass ratio be 1:100 bigarabe extract, with ferment stoste mass ratio be 1:20 synanthrin, with ferment stoste mass ratio be 1:100 ginger extract, be the potassium sorbate of 1:2000 and be the Cordyceps militaris extract of 1:10 with ferment stoste mass ratio with ferment stoste mass ratio, after stirring at the temperature of 115 DEG C high-temperature sterilization 30-50 minute, carry out packing afterwards.
2. a kind of Cordyceps militaris bigarabe ferment manufacturing process having fat-reducing effect according to claim 1, it is characterized in that, in described step 2, lactic acid bacteria is Bifidobacterium and streptococcus thermophilus, wherein Bifidobacterium dry bacterium powder 1 kilogram, streptococcus thermophilus dry bacterium powder 0.5 kilogram.
CN201510865283.0A 2015-12-01 2015-12-01 Preparation process of cordyceps militaris and bitter orange enzyme with weight loss effect Pending CN105495573A (en)

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