CN105495574A - Preparation method of cordyceps militaris and tomato enzyme with cosmetic effect - Google Patents
Preparation method of cordyceps militaris and tomato enzyme with cosmetic effect Download PDFInfo
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- CN105495574A CN105495574A CN201510865363.6A CN201510865363A CN105495574A CN 105495574 A CN105495574 A CN 105495574A CN 201510865363 A CN201510865363 A CN 201510865363A CN 105495574 A CN105495574 A CN 105495574A
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- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 29
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 16
- 239000002537 cosmetic Substances 0.000 title claims abstract description 13
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 240000003768 Solanum lycopersicum Species 0.000 title abstract description 12
- 108090000790 Enzymes Proteins 0.000 title abstract description 9
- 102000004190 Enzymes Human genes 0.000 title abstract description 9
- 230000000694 effects Effects 0.000 title abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 12
- 230000004151 fermentation Effects 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 12
- 241000186660 Lactobacillus Species 0.000 claims abstract description 6
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 4
- 239000000419 plant extract Substances 0.000 claims abstract description 4
- 239000007788 liquid Substances 0.000 claims description 30
- 241000894006 Bacteria Species 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000000706 filtrate Substances 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 12
- 238000010899 nucleation Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 238000011081 inoculation Methods 0.000 claims description 11
- 241000196324 Embryophyta Species 0.000 claims description 6
- 241001052560 Thallis Species 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 235000014655 lactic acid Nutrition 0.000 claims description 6
- 239000004310 lactic acid Substances 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 210000002966 serum Anatomy 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 241000186000 Bifidobacterium Species 0.000 claims description 5
- 244000298715 Actinidia chinensis Species 0.000 claims description 3
- 235000009434 Actinidia chinensis Nutrition 0.000 claims description 3
- 235000009436 Actinidia deliciosa Nutrition 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 241000235342 Saccharomycetes Species 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 3
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 3
- 240000006365 Vitis vinifera Species 0.000 claims description 3
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 235000015278 beef Nutrition 0.000 claims description 3
- 235000021028 berry Nutrition 0.000 claims description 3
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 229940002508 ginger extract Drugs 0.000 claims description 3
- 235000020708 ginger extract Nutrition 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 2
- 241000227653 Lycopersicon Species 0.000 claims 4
- 206010015150 Erythema Diseases 0.000 abstract description 3
- 239000011148 porous material Substances 0.000 abstract description 3
- 230000037394 skin elasticity Effects 0.000 abstract description 3
- 231100000321 erythema Toxicity 0.000 abstract description 2
- 230000004060 metabolic process Effects 0.000 abstract description 2
- 239000000243 solution Substances 0.000 abstract 2
- 239000011550 stock solution Substances 0.000 abstract 2
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000005457 optimization Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 6
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 101100256850 Drosophila melanogaster EndoA gene Proteins 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000002210 biocatalytic effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000008429 bread Nutrition 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000007969 cellular immunity Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a preparation method of a cordyceps militaris and tomato enzyme with a cosmetic effect. The method comprises specific steps as follows step one a yeast strain solution is prepared; step two a lactobacillus strain solution is prepared; step three fermentation culture is performed; step four an enzyme stock solution is obtained through centrifugal treatment; step five plant extract is added to the enzyme stock solution. The enzyme and the cordyceps militaris are mixed for fermentation or compounded according to respective characteristics, other active ingredients are added, and a cordyceps militaris enzyme product is innovatively developed. The product integrates efficacy of the enzyme and efficacy of the cordyceps militaris and has the efficacy of optimization of the metabolic process, beautifying, bodybuilding, improvement of skin elasticity, reduction of erythema in skin, shrinking of pores and the like.
Description
Technical field
The present invention relates to bio-science field, be specifically related to a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result.
Background technology
Ferment is also called " enzyme ", is the polymer substance with biocatalytic Activity.Nearly all cellular activity process all needs the participation of ferment.Modern society, along with the quickening of people's rhythm of life and the aggravation of environmental pollution, the content of people's body endo enzyme reduces day by day, and then reduces body immunity and metabolic rate, and the probability making people ill increases.At present, the ferment content in daily bread is too low, cannot meet the normal need of people.In order to meet modern to diversity, the balance of nutritional need be easy to absorbability, the production of efficient ferment product is extremely urgent.
Cordyceps militaris has the active component similar with Cordyceps sinensis and pharmacological action, is called as " Cordyceps sinensis " that can manually cultivate.2009, Cordyceps militaris was approved for new resource food, and its price is far below Cordyceps sinensis, was thus more and more subject to the favor of consumers in general.The traditional Chinese medical science is thought, Cordyceps militaris enters lung kidney two warp, and both tonifying lung was cloudy, and kidney-replenishing again, function cures mainly as invigorating the lung and the kidney, relieving cough and reducing sputum.Modern medicine study its there is cellular immunity and Humoral Immunological Regulation Roles, also there is antifatigue effect.Therefore, be the product of primary raw material if can develop with Cordyceps militaris, further developing of Cordyceps militaris industry can not only be promoted, more can bring happiness for the health of broad masses of the people.Although the product that on market, existing Cordyceps militaris is relevant, market scale is little, and added value is low, and effect is indefinite, needs badly and develops cordyceps militaris series products that is efficient, high added value.
Summary of the invention
The object of the invention is to, a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result is provided, with
Customer service is to existing method Problems existing.
The object of the invention is to be achieved through the following technical solutions: a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result, described in this, method comprises:
Step one: prepared by barms liquid, plant flow container and expand culture medium, YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and YPD fluid nutrient medium is put into 0.5M by 93% water
3seeding tank in, 115 DEG C, 30-50 minute high-temperature sterilization; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank in, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3M
3/ min, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by lactobacillus inoculation liquid, plants flow container and expands culture medium: enrichment medium of wort, its mass percent is: 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,87.8% water, puts into 0.5M by enrichment medium of wort
3in seeding tank, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 3 times under aseptic technique by high-temperature sterilization 30-50min at 115 DEG C, and bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: fermented and cultured, be the tomato of 100:60:40:1000 by mass ratio, grape, Kiwi berry, water mixing is smashed rear filtration and is obtained filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, stir, then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid,
Step 4: centrifugal treating obtains ferment stoste, and zymotic fluid is imported centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5: add plant extracts to ferment stoste, be that 10:0.15:12:20:1000 is mixed to join ferment stoste by Senna P.E, sucrose, ginger extract, Cordyceps militaris extract, stoste according to mass ratio, 115 degree of 30-50 minute high-temperature sterilizations, carry out packing.
Above-mentioned a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result, described speed of agitator is 100-120rpm/min.
Above-mentioned a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result, described lactic acid bacteria is Bifidobacterium.
The invention has the advantages that: compared with prior art, both, in conjunction with the features of ferment and Cordyceps militaris, are carried out mixed culture fermentation or composite, then are equipped with other active component, develop Cordyceps militaris ferment product innovatively by the present invention.Effect of this product set ferment and Cordyceps militaris, have optimize metabolic process, beauty treatment, body beautification, increase skin elasticity, reduce skin erythema, reduce the effects such as pore.
Detailed description of the invention
Below in conjunction with specific embodiment, technical scheme of the present invention is described in detail, to understand object, the scheme of the present invention further, but not as the restriction of claims protection domain of the present invention.
Embodiment 1.The invention discloses a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result, the method comprises:
Step one: prepared by barms liquid, plant flow container and expand culture medium, YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and YPD fluid nutrient medium is put into 0.5M by 93% water
3seeding tank in, 115 DEG C, 30-50 minute high-temperature sterilization; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank in, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3M
3/ min, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality.
Step 2: prepared by bifidobacterium species atcc liquid, plants flow container and expands culture medium: enrichment medium of wort, its mass percent is: 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,87.8% water, puts into 0.5M by enrichment medium of wort
3in seeding tank, 1.5 kilograms of Bifidobacterium dry bacterium powders are dissolved in the sterilized water of 3 times under aseptic technique by high-temperature sterilization 30-50min at 115 DEG C, and bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain required Bifidobacterium;
Step 3: fermented and cultured, be the tomato of 100:60:40:1000 by mass ratio, grape, Kiwi berry, water mixing is smashed rear filtration and is obtained filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, speed of agitator is 100-120rpm/min, then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid,
Step 4: centrifugal treating obtains ferment stoste, and zymotic fluid is imported centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5: add plant extracts to ferment stoste, be that 10:0.15:12:20:1000 is mixed to join ferment stoste by Senna P.E, sucrose, ginger extract, Cordyceps militaris extract, stoste according to mass ratio, 115 degree of 30-50 minute high-temperature sterilizations, carry out packing.
Embodiment 2.The evaluation and test volunteer participating in the Cordyceps militaris tomato ferment of cosmetic result has 16 people, and sooner or later respectively taking 1 time, each 100-200ml for each person every day, 15 days is an evaluation and test cycle.Compare before and after taking, the average color cellulose content decline 18-30% of 16 people, skin elasticity increases 22-32%, and moisture content of skin increases 8-13%, and pore dia reduces 10-20%, and erythema reduces 10-20%.
Above the detailed description of the invention of invention is described; but these explanations can not be understood to limit scope of the present invention; protection scope of the present invention is limited by the claims of enclosing, and any change on the claims in the present invention basis is all protection scope of the present invention.
Claims (3)
1. there is a Cordyceps militaris tomato ferment preparation method for cosmetic result, it is characterized in that: described method comprises:
Step one: prepared by barms liquid, plant flow container and expand culture medium, YPD fluid nutrient medium, its mass percent is 1% yeast extract, 2% peptone, 2% glucose, 2% agar, and YPD fluid nutrient medium is put into 0.5M by 93% water
3seeding tank in, 115 DEG C, 30-50 minute high-temperature sterilization; Under aseptic technique, 1.5 kilograms of saccharomycete dry bacterium powders are dissolved in the sterilized water of 3 times, form bacterium liquid, described bacterium liquid is loaded serum bottle and is inoculated into 0.5M
3seeding tank in, at 28-30 DEG C, pass into aseptic compressed air, throughput is 0.2-0.3M
3/ min, cultivates 20-30h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeats the kind liquid that above-mentioned steps can obtain the yeast liquid of required quality;
Step 2: prepared by lactobacillus inoculation liquid, plants flow container and expands culture medium: enrichment medium of wort, its mass percent is: 10% brewer's wort, 0.5% soy peptone, 0.5% yeast extract, 1% beef extract, 0.2%K
2hPO
4,87.8% water, puts into 0.5M by enrichment medium of wort
3in seeding tank, 1.5 kilograms of lactic acid bacteria dry bacterium powders are dissolved in the sterilized water of 3 times under aseptic technique by high-temperature sterilization 30-50min at 115 DEG C, and bacterium liquid loads serum bottle and is inoculated into 0.5M
3seeding tank, 37 DEG C of constant temperature quiescent culture 24-36h, until thalli growth to stopping during logarithmic phase latter stage cultivating, repeat the kind liquid that above-mentioned steps can obtain required lactic acid bacteria;
Step 3: fermented and cultured, be the tomato of 100:60:40:1000 by mass ratio, grape, Kiwi berry, water mixing is smashed rear filtration and is obtained filtrate, culture medium is made with described filtrate, high-temperature sterilization 30-50 minute at 115 DEG C, described culture medium is added in fermentation tank, then be the saccharomycetic kind of liquid that the inoculum concentration inoculation step 1 of the 5%-10% of described filtrate obtains by quality, 48h is cultivated at the temperature of 28 DEG C, the volume of described fermentation tank is V cubic meter, throughput is (0.4-0.6) V cubic meters per minute, stir, then be the lactobacillus inoculation liquid that the inoculum concentration inoculation step 2 of the 5%-10% of described filtrate obtains by quality, continue to cultivate 5-7d to fermentation ends at the temperature of 37 DEG C, obtain zymotic fluid,
Step 4: centrifugal treating obtains ferment stoste, and zymotic fluid is imported centrifuge, and through the centrifugal 10min of 6000rpm, gained supernatant is ferment stoste;
Step 5: add plant extracts to ferment stoste, be that 10:0.15:12:20:1000 is mixed to join ferment stoste by Senna P.E, sucrose, ginger extract, Cordyceps militaris extract, stoste according to mass ratio, 115 degree of 30-50 minute high-temperature sterilizations, carry out packing.
2. a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result according to claim 1, is characterized in that: described speed of agitator is 100-120rpm/min.
3. a kind of Cordyceps militaris tomato ferment preparation method having cosmetic result according to claim 1, is characterized in that: described lactic acid bacteria is Bifidobacterium.
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| CN201510865363.6A CN105495574A (en) | 2015-12-01 | 2015-12-01 | Preparation method of cordyceps militaris and tomato enzyme with cosmetic effect |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110692882A (en) * | 2019-11-06 | 2020-01-17 | 山东博华高效生态农业科技有限公司 | Tomato enzyme beverage with eucommia flavor and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110692882A (en) * | 2019-11-06 | 2020-01-17 | 山东博华高效生态农业科技有限公司 | Tomato enzyme beverage with eucommia flavor and preparation method thereof |
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