CN108618084A - A kind of mushroom enzymolysis powder and preparation method thereof - Google Patents

A kind of mushroom enzymolysis powder and preparation method thereof Download PDF

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Publication number
CN108618084A
CN108618084A CN201810438858.4A CN201810438858A CN108618084A CN 108618084 A CN108618084 A CN 108618084A CN 201810438858 A CN201810438858 A CN 201810438858A CN 108618084 A CN108618084 A CN 108618084A
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CN
China
Prior art keywords
powder
enzymolysis
mushroom
slurry
preparation
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CN201810438858.4A
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Chinese (zh)
Inventor
朱新成
马翠丽
李长春
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Zhongjing Food Ltd By Share Ltd
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Zhongjing Food Ltd By Share Ltd
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Priority to CN201810438858.4A priority Critical patent/CN108618084A/en
Publication of CN108618084A publication Critical patent/CN108618084A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof

Abstract

The invention discloses a kind of mushrooms to digest powder and preparation method thereof.The preparation method of mushroom enzymolysis powder includes the following steps:Raw material is crushed, feed powder is obtained;Feed powder and mixing enzyme powder are configured to 45 DEG C 65 DEG C of enzymolysis slurry;Enzymolysis slurry is digested into a period of time at 50 DEG C 65 DEG C;Destroy the enzyme treatment is carried out to the enzymolysis slurry that enzymolysis terminates;It to the enzymolysis slurry drying after enzyme deactivation and crushes, obtains mushroom enzymolysis powder.Mixing enzyme powder can disposably be put into and be digested by the disclosure, and easy to operate, it can be achieved that producing in enormous quantities, enzymolysis efficiency is high, and the delicate flavour material in mushroom can be released effectively.In addition, pH value need not be adjusted in enzymolysis process, be conducive to further reduce enzymolysis cost, improve enzymolysis efficiency.

Description

A kind of mushroom enzymolysis powder and preparation method thereof
Technical field
The present invention relates to edible fungi flavoring agent fields, and powder and preparation method thereof is digested more particularly, to a kind of mushroom.
Background technology
With the raising that people require quality of life, edible fungi flavoring agent has also obtained quick development.Especially Mushroom digests powder because easy to use, have many advantages, such as it is fresh fragrant and full of nutrition, it is extensively popular with users.
It, can after being reconciled with food rich in nutritional ingredients such as amino acid and polypeptides for digesting powder made of mushroom The fresh, fragrant of food, savoury is remarkably reinforced, improves the uncomfortable mouthfeel of single tasty agents.Mushroom enzymolysis powder can be used for flavor flavouring, The flavoring agents manufacture field or food processing etc. such as flavor pack, fillings, ham, a ball, snack food, delicate flavour essence fields.
But need repeatedly to throw enzyme in existing mushroom enzymolysis powder production process, and enzymolysis efficiency is relatively low, is also easy to produce big The residual waste of amount causes production cost higher.
Therefore, how to provide it is a kind of can once throw enzyme and enzymolysis efficiency it is high mushroom enzymolysis powder preparation method become this field The technical barrier of urgent need to resolve.
Invention content
It is an object of the present invention to provide a kind of preparation methods that can once throw enzyme and the high mushroom enzymolysis powder of enzymolysis efficiency New solution.
According to the first aspect of the invention, a kind of preparation method of mushroom enzymolysis powder is provided.
The preparation method of the mushroom enzymolysis powder of the present invention includes the following steps:
Step S1:Raw material is crushed, micron-sized feed powder is obtained;
Step S2:Feed powder and mixing enzyme powder are configured to 45 DEG C -65 DEG C of enzymolysis slurry, wherein mixing enzyme powder is disposable Input, and it includes neutral cellulase, alkali protease, compound protease and carbohydrase to mix enzyme powder;
Step S3:Enzymolysis slurry is digested into a period of time at 50 DEG C -65 DEG C;
Step S4:Enzymolysis slurry to digesting end in the step S3 carries out destroy the enzyme treatment;
Step S5:It to the enzymolysis slurry drying after enzyme deactivation in the step S4 and crushes, obtains mushroom enzymolysis powder.
Optionally, the raw material in the step S1 is mushroom stems.
Optionally, the granularity of the feed powder in the step S1 is 25 μm -50 μm.
Optionally, the step S2 is specific as follows:
Step S2-1:The slurry that will be obtained feed powder in the step S1 and be made into 50 DEG C -65 DEG C;
Step S2-2:Neutral cellulase, alkali protease, compound protease and carbohydrase are made into 45 DEG C -65 DEG C Enzyme solution;
Step S2-3:The enzyme solution matched in the slurry matched in the step S2-1 and the step S2-2 is mixed, is obtained To enzymolysis slurry.
Optionally, the solid-liquid ratio of the slurry in the step S2-1 is 1:(1-4).
Optionally, the compound protease in the step S2 includes the aspergillus oryzae and 35wt%- of 55wt%-65wt% The neutral proteinase of 45wt%.
Optionally, the quality of the neutral cellulase in the step S2 is the feed powder quality in the step S1 The quality of alkali protease in 0.5%-1.5%, the step S2 is the 0.3%- of the feed powder quality in the step S1 The quality of compound protease in 1%, the step S2 is the 0.5%-1.5% of the feed powder quality in the step S1, described The quality of carbohydrase in step S2 is the 0.5%-1% of the feed powder quality in the step S1.
Optionally, the time of the enzymolysis in the step S3 is 0.5h-1h.
According to the second aspect of the invention, a kind of mushroom enzymolysis powder is additionally provided.
The preparation method that the mushroom enzymolysis powder of the present invention is digested powder by the mushroom of the present invention is made.
Optionally, the mushroom enzymolysis powder further includes salt, and mass fraction of the salt in the mushroom digests powder is more than 0 And it is less than or equal to 1%.
According to one embodiment of the disclosure, mixing enzyme powder can disposably be put into and be digested, it is easy to operate, it can be real Now produce in enormous quantities, enzymolysis efficiency is high, and the delicate flavour material in mushroom can be released effectively.In addition, need not be adjusted in enzymolysis process Whole pH value is conducive to further reduce enzymolysis cost, improves enzymolysis efficiency.
By referring to the drawings to the detailed description of exemplary embodiment of the present invention, other feature of the invention and its Advantage will become apparent.
Description of the drawings
It is combined in the description and the attached drawing of a part for constitution instruction shows the embodiment of the present invention, and even With its explanation together principle for explaining the present invention.
Fig. 1 is the flow chart for the preparation method embodiment that disclosure mushroom digests powder.
Specific implementation mode
Carry out the various exemplary embodiments of detailed description of the present invention now with reference to attached drawing.It should be noted that:Unless in addition having Body illustrates that the unlimited system of component and the positioned opposite of step, numerical expression and the numerical value otherwise illustrated in these embodiments is originally The range of invention.
It is illustrative to the description only actually of at least one exemplary embodiment below, is never used as to the present invention And its application or any restrictions that use.
Technology, method and apparatus known to person of ordinary skill in the relevant may be not discussed in detail, but suitable In the case of, the technology, method and apparatus should be considered as part of specification.
In shown here and discussion all examples, any occurrence should be construed as merely illustrative, without It is as limitation.Therefore, other examples of exemplary embodiment can have different values.
It should be noted that:Similar label and letter indicate similar terms in following attached drawing, therefore, once a certain Xiang Yi It is defined, then it need not be further discussed in subsequent attached drawing in a attached drawing.
It needs repeatedly to throw enzyme and the relatively low problem of enzymolysis efficiency, this hair during in order to solve to prepare mushroom enzymolysis powder It is bright to provide a kind of preparation method of mushroom enzymolysis powder.
As shown in Figure 1, the preparation method of the mushroom enzymolysis powder of the disclosure includes the following steps:
Step S1:Raw material is crushed, micron-sized feed powder is obtained.The granularity of feed powder can flexibly be selected according to actual demand.Example Such as, it is 25 μm -50 μm raw material to be crushed to granularity.
Raw material such as can be picked before crushing, be cleaned, being dried at the processing, to ensure the purity of feed powder after crushing.With For mushroom, in general, it is miscellaneous to remove the mushroom that damages by worms, rot mushroom and plastic skin, hair, stone etc. first can pick processing to mushroom Matter foreign matter, then the net material picked out is subjected to rinsing processing through bubble washer, the net material for rinsing completion is set through tunnel type drying Standby to be dried, drying is yellowish-white to mushroom color and luster, and moisture completes the preceding processing of raw material below 4%.
Above-mentioned raw materials can be the mushroom, such as mushroom fruiting body complete stool etc. of whole strain.Particularly, using mushroom stems as former Material.Mushroom stems are usually the by-product in mushroom commercial treatment, account for about the 15%-25% of the whole strain quality of dried thin mushroom.Mushroom Handle is made of mushroom mycelium as mushroom cap, equally contain the nutrition such as abundant protein, amino acid, lentinan and Functional component, but contain abundant dietary fiber in mushroom stems, palatability is poor, eat it is limited, value cannot embody and mostly by It abandons, results in waste of resources as waste.Therefore, using mushroom stems the production of mushroom enzymolysis powder is advantageously reduced as raw material Cost fully utilizes the mushroom handle for needing to abandon as waste originally.
Step S2:Feed powder and mixing enzyme powder are configured to 45 DEG C -65 DEG C of enzymolysis slurry, wherein mixing enzyme powder is disposable Input, and it includes neutral cellulase, alkali protease, compound protease and carbohydrase to mix enzyme powder.When it is implemented, can incite somebody to action Feed powder is first made into slurry, and mixing enzyme powder is made into enzyme solution, and then enzyme solution is added in slurry or slurry is added in enzyme solution and is prepared Obtain enzymolysis slurry;Alternatively, first can fully dissolve mixing enzyme powder, then puts into feed powder and prepare to obtain enzymolysis slurry;It again alternatively, can Mixing enzyme powder is direct plungeed into the slurry that feed powder is made into, preparation obtains enzymolysis slurry.
Step S3:Enzymolysis slurry is digested into a period of time at 50 DEG C -65 DEG C.Optionally, the temperature of enzymolysis can be 55 DEG C- 60 DEG C, the time of enzymolysis can be 0.5h-1h.
In order to ensure efficiently to digest, constantly enzymolysis slurry can be stirred in enzymolysis process.The rotating speed of stirring It is smaller than and is equal to 100rpm.
Step S4:Enzymolysis slurry to digesting end in step S3 carries out destroy the enzyme treatment.This field can be used in destroy the enzyme treatment Well known mode is realized.Optionally, the condition of destroy the enzyme treatment is:95 DEG C -105 DEG C, 10min-20min.
Step S5:It to the enzymolysis slurry drying after enzyme deactivation in step S4 and crushes, obtains mushroom enzymolysis powder.Mushroom digests powder Granularity is optionally 25 μm -50 μm.
The preparation method of the mushroom enzymolysis powder of the disclosure, which can disposably put into mixing enzyme powder, to be digested, easy to operate, It can be achieved to produce in enormous quantities, enzymolysis efficiency is high, and the delicate flavour material in mushroom can be released effectively.In addition, being not required in enzymolysis process PH value is adjusted, is conducive to further reduce enzymolysis cost, improves enzymolysis efficiency.
Optionally, step S2 is specific as follows:
Step S2-1:The slurry that will be obtained feed powder in step S1 and be made into 50 DEG C -65 DEG C.Further, the temperature of slurry can Control is at 55 DEG C -60 DEG C.When specific operation, the temperature of demand can be first heated water to, then feed powder is put into water so that material is made Slurry.
In order to ensure the viscosity of slurry and the efficiency of enzymolysis, the water content of controllable slurry, for example, the material of control slurry Liquor ratio is 1:(1-4).It is 1 to select the solid-liquid ratio of slurry:When (1-4) also helps the drying process for shortening follow-up enzymolysis slurry Between, reduce the production cost of enzymolysis powder.
Step S2-2:Neutral cellulase, alkali protease, compound protease and carbohydrase are made into 45 DEG C -65 DEG C Enzyme solution.Further, the temperature for controlling enzyme solution is 50 DEG C -55 DEG C.
Step S2-3:The enzyme solution matched in the slurry and step S2-2 matched in step S2-1 is mixed, enzymolysis powder is obtained Slurry.Optionally, enzyme solution is put into slurry, to ensure that the two is uniformly mixed together.
In order to ensure enzymolysis efficiency, the compound protease in step S2 may include 55wt%-65wt% aspergillus oryzae and The neutral proteinase of 35wt%-45wt%.
In order to ensure that enzymolysis efficiency, the quality of the neutral cellulase in step S2 are the feed powder quality in step S1 The quality of alkali protease in 0.5%-1.5%, step S2 is the 0.3%-1%, step S2 of the feed powder quality in step S1 In compound protease quality be the feed powder quality in step S1 0.5%-1.5%, the quality of the carbohydrase in step S2 For the 0.5%-1% of the feed powder quality in step S1.
Mushroom enzymolysis powder delicate flavour foot made from the preparation method of powder is digested using disclosure mushroom, it is easy to use.
Further, mushroom enzymolysis powder further includes salt, and mass fraction of the salt in mushroom digests powder is more than 0 and is less than or equal to 1%.By the addition of a small amount of salt, is conducive to the purity for also ensureing mushroom enzymolysis powder while prominent mushroom enzymolysis powder delicate flavour, is convenient for Client uses.
The raw material used and production equipment in following embodiment or comparative example can be by commercially available acquisitions, and the disclosure is to this It is not further described.
Embodiment 1
(1) it is rinsed after pick processing to mushroom stems and dry, drying is yellowish-white to mushroom handle color and luster, has peculiar mushroom handle fragrant Gas, moisture is below 4%.
(2) the mushroom handle of drying is crushed to the feed powder for 25 μm.
(3) feed powder for weighing 100kg is added in 100kg water, and is heated to 55 DEG C and matches to obtain slurry, under quick stirring Disposable input neutral cellulase 1.5kg, alkali protease 1kg, compound protease 1.5kg, carbohydrase 1kg, it is lasting to stir To being uniformly mixed without powder agglomates, match to obtain enzymolysis slurry.
(4) slurry will be digested at 50 DEG C, digested under conditions of speed of agitator 100rmp, heat preservation 1h.
(5) destroy the enzyme treatment is carried out to enzymolysis slurry.
(6) it is crushed after enzymolysis slurry being dried under vacuum to moisture below 4%, obtains 25 μm of mushroom enzymolysis powder.
Embodiment 2
(1) it is rinsed after pick processing to mushroom stems and dry, drying is yellowish-white to mushroom handle color and luster, has peculiar mushroom handle fragrant Gas, moisture is below 4%.
(2) the mushroom handle of drying is crushed to the feed powder for 50 μm.
(3) feed powder for weighing 100kg is added 55 DEG C, in 350kg water, with obtaining slurry;Weigh neutral cellulase 0.5kg, Alkali protease 0.3kg, compound protease 0.5kg, carbohydrase 0.5kg are added 55 DEG C, in the water of 50kg, with obtaining enzyme solution;By enzyme Liquid puts into slurry and is uniformly mixed, and matches to obtain enzymolysis slurry.
(4) slurry will be digested at 50 DEG C, digested under conditions of speed of agitator 50rmp, heat preservation 0.5h.
(5) destroy the enzyme treatment is carried out to enzymolysis slurry.
(6) 25 μm are crushed to after enzymolysis slurry being dried under vacuum to moisture below 4%, the salt for mixing 1wt% is made The mushroom obtained digests powder.
Embodiment 3
(1) it is rinsed after pick processing to mushroom stems and dry, drying is yellowish-white to mushroom handle color and luster, has peculiar mushroom handle fragrant Gas, moisture is below 4%.
(2) the mushroom handle of drying is crushed to the feed powder for 40 μm.
(3) feed powder for weighing 100kg is added 55 DEG C, in 250kg water, with obtaining slurry;Weigh neutral cellulase 1kg, alkali Property proteinase-10 .5kg, compound protease 0.8kg, carbohydrase 0.7kg be added 55 DEG C, in the water of 50kg, with obtaining enzyme solution;By enzyme solution It puts into slurry and is uniformly mixed, match to obtain enzymolysis slurry.
(4) slurry will be digested at 50 DEG C, digested under conditions of speed of agitator 100rmp, heat preservation 0.75h.
(5) destroy the enzyme treatment is carried out to enzymolysis slurry.
(6) 35 μm are crushed to after enzymolysis slurry being dried under vacuum to moisture below 4%, mixes the salt system of 0.5wt% The mushroom obtained digests powder.
Although some specific embodiments of the present invention are described in detail by example, the skill of this field Art personnel it should be understood that example above merely to illustrating, the range being not intended to be limiting of the invention.The skill of this field Art personnel are it should be understood that can without departing from the scope and spirit of the present invention modify to above example.This hair Bright range is defined by the following claims.

Claims (10)

1. a kind of preparation method of mushroom enzymolysis powder, which is characterized in that include the following steps:
Step S1:Raw material is crushed, micron-sized feed powder is obtained;
Step S2:Feed powder and mixing enzyme powder are configured to 45 DEG C -65 DEG C of enzymolysis slurry, wherein mixing enzyme powder is disposably put into, And mixing enzyme powder includes neutral cellulase, alkali protease, compound protease and carbohydrase;
Step S3:Enzymolysis slurry is digested into a period of time at 50 DEG C -65 DEG C;
Step S4:Enzymolysis slurry to digesting end in the step S3 carries out destroy the enzyme treatment;
Step S5:It to the enzymolysis slurry drying after enzyme deactivation in the step S4 and crushes, obtains mushroom enzymolysis powder.
2. the preparation method of mushroom enzymolysis powder according to claim 1, which is characterized in that the raw material in the step S1 is perfume Mushroom handle.
3. the preparation method of mushroom enzymolysis powder according to claim 1, which is characterized in that the grain of the feed powder in the step S1 Degree is 25 μm -50 μm.
4. the preparation method of mushroom enzymolysis powder according to claim 1, which is characterized in that the step S2 is specific as follows:
Step S2-1:The slurry that will be obtained feed powder in the step S1 and be made into 50 DEG C -65 DEG C;
Step S2-2:Neutral cellulase, alkali protease, compound protease and carbohydrase are made into 45 DEG C -65 DEG C of enzyme solution;
Step S2-3:The enzyme solution matched in the slurry matched in the step S2-1 and the step S2-2 is mixed, enzyme is obtained Solve slurry.
5. the preparation method of mushroom according to claim 4 enzymolysis powder, which is characterized in that slurry in the step S2-1 Solid-liquid ratio is 1:(1-4).
6. the preparation method of mushroom enzymolysis powder according to claim 1, which is characterized in that the compound protein in the step S2 Enzyme includes the aspergillus oryzae of 55wt%-65wt% and the neutral proteinase of 35wt%-45wt%.
7. the preparation method of the mushroom enzymolysis powder according to any one of claim 1 to 6, which is characterized in that the step S2 In neutral cellulase quality be the feed powder quality in the step S1 0.5%-1.5%, the alkali in the step S2 Property protease quality be the feed powder quality in the step S1 0.3%-1%, compound protease in the step S2 Quality is the 0.5%-1.5% of the feed powder quality in the step S1, and the quality of the carbohydrase in the step S2 is the step The 0.5%-1% of feed powder quality in rapid S1.
8. the preparation method of mushroom according to claim 1 enzymolysis powder, which is characterized in that enzymolysis in the step S3 when Between be 0.5h-1h.
9. a kind of mushroom digests powder, which is characterized in that the mushroom enzymolysis powder is digested by the mushroom described in any one of claim 1 to 8 The preparation method of powder is made.
10. mushroom according to claim 9 digests powder, which is characterized in that the mushroom enzymolysis powder further includes salt, the salt Mass fraction in the mushroom digests powder is more than 0 and is less than or equal to 1%.
CN201810438858.4A 2018-05-09 2018-05-09 A kind of mushroom enzymolysis powder and preparation method thereof Pending CN108618084A (en)

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Application publication date: 20181009