CN107319304A - A kind of edible mushroom enzymolysis process and its application for preparing domestic fungus flour - Google Patents
A kind of edible mushroom enzymolysis process and its application for preparing domestic fungus flour Download PDFInfo
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- CN107319304A CN107319304A CN201710672143.0A CN201710672143A CN107319304A CN 107319304 A CN107319304 A CN 107319304A CN 201710672143 A CN201710672143 A CN 201710672143A CN 107319304 A CN107319304 A CN 107319304A
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- edible mushroom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
- A23L7/10—Cereal-derived products
- A23L7/198—Dry unshaped finely divided cereal products, not provided for in groups A23L7/117 - A23L7/196 and A23L29/00, e.g. meal, flour, powder, dried cereal creams or extracts
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
Abstract
The invention belongs to technical field of food deep processing, and in particular to a kind of edible mushroom enzymolysis process, and further disclose its application for being used to prepare domestic fungus flour.Extraction edible mushroom enzymolysis process of the present invention, immobilization complex enzyme is prepared by the way that cellulase, protease are fixed on magnetic inorganic material support, and and then progress enzyme digestion reaction.Because the constituent of edible mushroom cell membrane mainly has cellulose, glucan, chitin etc., they are interleaved with each other into the fine and close network structure for being different from other plant, if directly using protease hydrolyzed edible mushroom, the nutriments such as intracellular amino acid, nucleotides can be prevented from discharging completely.The present invention is digested using cellulase, proteinase synergy effect, accelerate the decomposition of cell membrane, broken, shortening enzymolysis time, improve enzymolysis efficiency, the nutriment included in edible mushroom, delicate flavour material etc. is set fully to discharge, nutrition and characteristic flavor on basis more enrich.
Description
Technical field
The invention belongs to technical field of food deep processing, and in particular to a kind of edible mushroom enzymolysis process, and further disclose
It is used for the application for preparing domestic fungus flour.
Background technology
Edible mushroom refers to that fructification is very large, gill fungus bacterium (macro fungi) that can be edible, is commonly referred to as mushroom.Chinese known food
Have kind more than 350 with bacterium, wherein many category Basidiomycotinas, common edible mushroom have mushroom, straw mushroom, mushroom, agaric, white fungus, hedgehog hydnum,
Dictyophora phalloidea, Trichotoma matsutake (matsutake), dried mushroom, russule, ganoderma lucidum, cordyceps sinensis, truffle, Pleurotus nebrodensis and bolete etc.；It is sub- that minority belongs to sac fungus
Door, mainly there is hickory chick, saddle fungus, ferfas etc..Above-mentioned fungi is grown in different regional, different ecological environments respectively.
Edible mushroom because containing Multiple components such as abundant carbohydrate, protein, several amino acids, steroidals, and
With the multiple biological activities such as antitumor, anti-oxidant, antibacterial, liver protection；Meanwhile, edible mushroom delicious flavour, the accessory substance of edible mushroom
Rich in dietary fibers such as beta glucan, chitin, hemicellulose, mannoses, with abundant nutrition and higher medical value,
And turn into recent years by the food materials of extensive concern, and liked by people, more as the common food materials on daily dining table.
At present, the edible application of edible mushroom is mainly to be added in food and plays its healthcare function and lift food product again
Matter.Noodles, steamed bun, bread etc. are a kind of daily staple foods that our people extremely likes, but the making of traditional wheaten food is present
The problems such as technique is dull simply, taste is more single, trophic component is not reasonable, can not meet people to health diet
Requirement.Proper amount of edible bacterium powder is added in flour and makes noodles, steamed bun, bread, master of the nutritive value compared with horn of plenty is made
Food, can not only improve flour mouthfeel, and add the content of dietary fiber in flour；Edible mushroom contains a variety of human body institutes simultaneously
The amino acid needed, wherein lysine, leucine content is higher, can make up the relatively low situation of lysine content in flour, improves people
Absorption and utilization of the body to dietary proteins, can improve the Dietary Health of China resident.So how edible mushroom to be led with these
How food, which preferably combines, has become one of hot research direction of China's food.But, current major part domestic fungus flour
The processing of product is primary processing, and it has simply carried out the drying of routine, pulverization process to edible fungi raw materials, matches therewith
Edible mushroom intensive processing technology but relative rarity, the utilization to beneficiating ingredients such as the nutriment in edible mushroom, delicate flavour materials
Degree is relatively low；Especially edible mushroom intensive processing technology is used to produce domestic fungus flour and staple food products, will be turned into the neck
The strong supplement of field technique and the market vacancy.
The content of the invention
Therefore, the technical problems to be solved by the invention are to provide a kind of edible mushroom enzymolysis process, to solve existing skill
The problem of edible fungi nutrition matter utilization degree is relatively low in art.
In order to solve the above technical problems, a kind of edible mushroom enzymolysis process of the present invention, comprises the following steps：
(1) pretreatment of raw material：Take dry edible mushroom to crush, obtain powder of edible fungus, and by the powder of edible fungus system of adding water
Edible mushroom feed liquid is obtained, it is standby；
(2) preparation of immobilization complex enzyme：Take inorganic carrier to add activator and carry out crosslinking activation processing, and add fiber
Plain enzyme and protease are mixed, and reaction is fixed to neutrality in regulation reaction solution pH, and separation of solid and liquid takes precipitation drying, produces described solid
Surely complex enzyme is changed, it is standby；
(3) the immobilization complex enzyme is added into the edible mushroom feed liquid to mix, and enzyme digestion reaction is carried out in 37-60 DEG C,
Obtain edible mushroom enzymolysis liquid；
(4) enzymolysis liquid obtained by step (3) is subjected to destroy the enzyme treatment, and the enzymolysis liquid is spray-dried, that is, obtained
Required edible mushroom digests powder.
The activator is glutaraldehyde solution.
In the step (2), the inorganic carrier is magnetic hydrotalcite or magnetic Fe3O4Nano particle.
The addition of the protease accounts for the 0.02%-0.2wt% of the edible mushroom feed liquid, the addition of the cellulase
Amount accounts for the 0.02%-0.2wt% of the edible mushroom feed liquid.
The protease include papain, flavor protease, compound protease, neutral proteinase, trypsase or
At least one of bromelain.
In the step (3), the enzymolysis step is carried out under ultrasonic synergistic microwave radiation technology；
The frequency of the ultrasonication is 40-60kHz, and power is 150-500W, and ultrasonic treatment time is 1min-
10min；
The power of the microwave treatment is 150-350W, and microwave treatment time is 1-10min.
In the step (3), the addition of the immobilization complex enzyme accounts for the 0.1- of the edible mushroom feed liquid quality
0.5wt%.
In the step (1), the powder of edible fungus is according to 1：10-1：The edible mushroom feed liquid is made in 40 solid-liquid ratio, and
The edible mushroom material liquid pH is adjusted for 6-7.
In the step (4), the condition of spray drying is：Feeding temperature be 100 DEG C -200 DEG C, leaving air temp be 80 DEG C -
95 DEG C, feeding speed is 10-50mL/min.
The edible mushroom includes at least one in White mushroom, pleurotus eryngii, mushroom, asparagus, flat mushroom, seafood mushroom or crab flavour mushroom
Kind.
Powder is digested the invention also discloses the edible mushroom as made from described edible mushroom enzymolysis process.
The invention also discloses the purposes that described edible mushroom enzymolysis powder is used to prepare domestic fungus flour.
The invention also discloses a kind of domestic fungus flour, including：
Described edible mushroom enzymolysis powder 0.5%-20wt%；
Wheat flour 80-99.5wt%.
Extraction edible mushroom enzymolysis process of the present invention, by the way that cellulase, protease are fixed into magnetic inorganic material
Immobilization complex enzyme, and and then progress enzyme digestion reaction are prepared on carrier.Because the constituent of edible mushroom cell membrane mainly has fibre
Element, glucan, chitin etc. are tieed up, they are interleaved with each other into the fine and close network structure for being different from other plant, if directly using egg
White enzyme enzymolysis edible mushroom, can prevent the nutriments such as intracellular amino acid, nucleotides from discharging completely.The present invention is used
Cellulase, proteinase synergy effect are digested, and are accelerated the decomposition of cell membrane, broken, shortening enzymolysis time, are improved enzymolysis effect
Rate, makes the nutriment included in edible mushroom, delicate flavour material etc. fully discharge, and nutrition and characteristic flavor on basis more enrich.
The more excellent use magnetic inorganic material of the present invention is prepared cellulase, protease as the carrier of immobilised enzymes
Digested into immobilised enzymes, it is to avoid free state enzyme in use in the presence of consumption enzyme amount is big, difficult separation, can not repeat to make
With, reaction time is long the shortcomings of.Although enzyme immobilization is that organized enzyme is fettered or is limited in necessarily using certain solid material
In region, but enzyme still is able to carry out itself distinctive catalytic reaction, and a kind of recyclable and recycling new technology.With tradition
Resolvase compare, immobilised enzymes is easily separated with substrate, simplifies purification step, and product yield is higher；Immobilised enzymes can be multiple
Recycling, reduces cost；Meanwhile, immobilised enzymes reaction condition is easily controllable, can successive reaction, and more suitable for multienzyme
Reaction.
The magnetic nanoparticle that the present invention is used is a kind of new composite, with its unique property, superparamagnetic
Property and good biocompatibility, it is had a wide range of applications in biological, medicine and field of Environment Protection.Made with magnetic nanoparticle
For fixed enzyme vector, with high mechanical strength, good heat endurance, anti-organic solvent, it is anticorrosive, easy to operate, be easy to separate, point
From it is thorough the advantages of.
The method of the invention, using ultrasonic synergistic microwave cooperating assistance enzymolysis, fuel factor that wherein ultrasonic wave is produced,
Mechanical effect and cavitation effect can cause fungal cell wall is isostructural to destroy and then promote the nutriments such as amino acid
Dissolution；Microwave radiation exaraction also has other the non-thermal effects acted on because of microwave polar molecule under the fuel factor produced and microwave field
Should, it is obvious to the extraction effect of nutriment in edible mushroom.Ultrasonic-microwave synergy can be that enzymolysis efficiency is carried significantly
Height, and with energy consumption is low, the features such as do not destroy active ingredient.And the inventive method is used as immobilised enzymes by the use of magnetic ball nano material
Carrier, also has the advantages that efficient absorption microwave radiation energy under microwave radiation technology, and complex enzyme is fixed on to synthetic magnetic
On nano particle, under microwave radiation, the efficiency of enzymolysis can be greatly enhanced.Compared to traditional enzyme solution, the enzymolysis
Technology will foreshorten to 15s the time required to proteolysis from 12h, and hydrolysis result is better than traditional in-solution digestion method.
The domestic fungus flour local flavor digested using edible mushroom of the present invention obtained by powder is delicious, nutritious.Identical
Addition under it is higher by more than 50% than the directly domestic fungus flour nutrient content that is made of addition powder of edible fungus.The starch of powder of edible fungus
Class content of material is few, the dietary fiber and polysaccharose substance rich content of strong absorptive, and lacks this kind of material in wheat flour,
The formation of albumen network, causes the gelatinizing qualitative change of dough poor in this serious water suction gelatinization for hindering starch and dough.Edible mushroom
Rich in protein in powder, but in the absence of mucedin, i.e. glutenin and gliadin, it is impossible to as wheat flour one
Sample forms the three-dimensional net structure of mucedin during dough is made.Therefore addition powder of edible fungus can reduce adding for flour
Work characteristic.Add edible mushroom enzymolysis powder of the present invention, under the premise of domestic fungus flour nutrient content identical is ensured, addition
Will be far less than the content for adding common powder of edible fungus.Therefore, under the premise of nutrient content identical, food of the present invention is added
The processing characteristics for digesting the domestic fungus flour that powder makes with bacterium are better than common domestic fungus flour, for improving adding for domestic fungus flour
Work characteristic has good effect.Meanwhile, the domestic fungus flour digested using edible mushroom of the present invention obtained by powder is widely used,
Available for staple food products such as making edible mushroom noodle, steamed bun, bread, it can also be used to make the leisure food such as edible mushroom biscuit, crispy rice
Product.
Domestic fungus flour of the present invention is processed by fruit body of edible fungi, it is adaptable to multiple eating bacterium raw material, is applicable
It is in extensive range, while can be difficult to sell as dry product or fresh goods using some residual mushrooms, secondary mushroom, stem, bacterium pin or mushroom root etc.
Edible mushroom and leftover bits and pieces be processed, improve the value of mushroom industry accessory substance, green energy conservation.
Embodiment
Embodiment 1
Edible mushroom enzymolysis powder extracts obtained as follows described in the present embodiment：
(1) edible mushroom fresh goods (mushroom) 2000g is weighed, classification is cleaned, the sick mushroom of bad mushroom etc. in silt and edible mushroom is removed
The part of inedibility, edible mushroom fresh goods is suitably cut, is uniformly positioned in air dry oven, 45 DEG C of drying 24h；After drying
Sample through general-purpose pulverizer crushed 60 mesh sieves be made edible fungus dry powder；Accurate weighing edible fungus dry powder 100g, with 1：20
Solid-liquid ratio adds a certain amount of drinking water, and edible mushroom feed liquid is made, standby；
(2) preparation of immobilization complex enzyme：Magnetic hydrotalcite nano particle is added the penta of deionized water and 2mL 0.5%
Stirring crosslinking activates 30min in dialdehyde solution, after centrifugation, washing for several times, adds cellulase and (accounts for the edible mushroom feed liquid
0.02%wt%), papain (0.2wt% for accounting for the edible mushroom feed liquid) and pH7.0 phosphate buffer solution, are placed in perseverance
Temperature vibration case is at 15 DEG C, and 200r/min speed oscillations are reacted 22 hours, are centrifuged after taking-up, be washed to washing lotion it is limpid it is colourless untill,
Supernatant is removed, then pellet frozen is dried, immobilization complex enzyme is produced；
(3) into the edible mushroom feed liquid, addition accounts for the immobilization complex enzyme of the edible mushroom feed liquid quality 0.1wt%, adjusts
PH is that 7.0, temperature is lasting stirring enzymolysis 2 hours under conditions of 37 DEG C；At the same time, in enzymolysis process carry out ultrasonic wave-
Microwave radiation technology acts synergistically, and the condition of the ultrasonic wave is that supersonic frequency 60kHz, power are 150W, and ultrasonic treatment time is
5min；The microwave condition be microwave power 300W, microwave time be 5min；
(4) enzymolysis liquid obtained by step (3) handle into 15min progress destroy the enzyme treatments in 100 DEG C of water-baths, and by edible mushroom enzyme
Solution liquid, which be spray-dried, obtains edible mushroom enzymolysis powder, and spray drying condition is：Feeding temperature is 200 DEG C, and leaving air temp is 95
DEG C, feeding speed is 30mL/min.
Embodiment 2
Edible mushroom enzymolysis powder extracts obtained as follows described in the present embodiment：
(1) edible mushroom fresh goods (mushroom) 2000g is weighed, classification is cleaned, the sick mushroom of bad mushroom etc. in silt and edible mushroom is removed
The part of inedibility, edible mushroom fresh goods is suitably cut, is uniformly positioned in air dry oven, 55 DEG C of drying 20h；After drying
Sample through general-purpose pulverizer crushed 60 mesh sieves be made edible fungus dry powder；Accurate weighing edible fungus dry powder 100g, with 1：30
Solid-liquid ratio adds a certain amount of drinking water, and edible mushroom feed liquid is made；
(2) preparation of immobilization complex enzyme：Magnetic hydrotalcite nano particle is taken to add the penta of deionized water and 2mL 0.5%
Stirring crosslinking activates 30min in dialdehyde solution, through centrifugation, washs for several times, adds cellulase and (account for the edible mushroom feed liquid
0.2%wt%), papain (0.01%wt% for accounting for the edible mushroom feed liquid), flavor protease (account for the edible fungus
The 0.01%wt% of liquid) and pH7.0 phosphate buffer solution, be placed in constant temperature oscillation case at 12 DEG C, 150r/min speed oscillations are anti-
Answer 24 hours, centrifuged after taking-up, be washed to washing lotion it is limpid it is colourless untill, remove supernatant, then pellet frozen dried, produced solid
Surely complex enzyme is changed；
(3) into the edible mushroom feed liquid, addition accounts for the immobilization complex enzyme of the edible mushroom feed liquid quality 0.1%, in pH
For 7.0, temperature is lasting stirring enzymolysis 2 hours under conditions of 40 DEG C；At the same time, ultrasonic wave-micro- is carried out in enzymolysis process
Ripple auxiliary collaboration processing, ultrasound condition is that supersonic frequency 50kHz, power are 300W, and ultrasonic treatment time is 8min；Micro-strip
Part be microwave power 200W, microwave time be 10min；
(4) enzymolysis liquid obtained by step (3) handle into 15min progress destroy the enzyme treatments and by edible mushroom enzyme in 100 DEG C of water-baths
Solution liquid, which be spray-dried, obtains edible mushroom enzymolysis powder, and spray drying condition is：Feeding temperature is 150 DEG C, and leaving air temp is 90
DEG C, feeding speed is 20mL/min.
Embodiment 3
Edible mushroom enzymolysis powder is extracted as follows described in the present embodiment：
(1) edible mushroom fresh goods (asparagus) 2000g is weighed, classification is cleaned, the sick mushroom of bad mushroom in silt and edible mushroom is removed
Deng the part of inedibility, edible mushroom fresh goods is suitably cut, is uniformly positioned in air dry oven, 60 DEG C of drying 24h；Drying
Sample afterwards crushed 60 mesh sieves through general-purpose pulverizer and edible fungus dry powder is made；Accurate weighing edible fungus dry powder 100g, with 1：40
Solid-liquid ratio add a certain amount of drinking water, edible mushroom feed liquid needed for being made；
(2) preparation of immobilization complex enzyme：By magnetic Fe3O4Nano particle adds the penta 2 of deionized water and 2mL0.5%
Stirring crosslinking activates 30min in aldehyde solution, and centrifugation, washing for several times, add cellulase and (account for the 0.1% of the edible mushroom feed liquid
Wt%), bromelain (0.05%wt% for accounting for the edible mushroom feed liquid), neutral proteinase (account for the edible mushroom feed liquid
0.05%wt%) with pH7.0 phosphate buffer solution, constant temperature oscillation case is placed in 15 DEG C, 300r/min speed oscillations reaction 16
Hour, centrifuged after taking-up, be washed to washing lotion it is limpid it is colourless untill, remove supernatant, then pellet frozen dried, produce immobilization
Complex enzyme；
(3) into the edible mushroom feed liquid, addition accounts for the immobilization complex enzyme of the edible mushroom feed liquid quality 0.2%, in pH
For 6.5, temperature is lasting stirring enzymolysis 2 hours under conditions of 45 DEG C；At the same time, ultrasonic wave-micro- is carried out in enzymolysis process
Ripple collaboration is aided in, and ultrasound condition is：Supersonic frequency 40kHz, power are 500W, and ultrasonic treatment time is 3min；Microwave condition
For：Microwave power 150W, microwave time are 10min；
(4) enzymolysis liquid obtained by step (3) handle into 15min progress destroy the enzyme treatments and by edible mushroom enzyme in 100 DEG C of water-baths
Solution liquid, which be spray-dried, obtains edible mushroom enzymolysis powder, and spray drying condition is：Feeding temperature is 120 DEG C, and leaving air temp is 85
DEG C, feeding speed is 20mL/min.
Embodiment 4
Edible mushroom enzymolysis powder is extracted as follows described in the present embodiment：
(1) edible mushroom fresh goods (flat mushroom) 2000g is weighed, classification is cleaned, the sick mushroom of bad mushroom etc. in silt and edible mushroom is removed
The part of inedibility, edible mushroom fresh goods is suitably cut, is uniformly positioned in air dry oven, 60 DEG C of drying 24h；After drying
Sample through general-purpose pulverizer crushed 60 mesh sieves be made edible fungus dry powder；Accurate weighing edible fungus dry powder 100g, with 1：10
Solid-liquid ratio adds a certain amount of drinking water, edible mushroom feed liquid needed for being made；
(2) preparation of immobilization complex enzyme：By magnetic Fe3O4Nano particle adds the penta 2 of deionized water and 2mL0.5%
Stirring crosslinking activates 30min in aldehyde solution, and centrifugation, washing for several times, add cellulase and (account for the 0.1% of the edible mushroom feed liquid
Wt%), flavor protease (0.05%wt% for accounting for the edible mushroom feed liquid), papain (account for the edible mushroom feed liquid
0.05%wt%) with pH7.0 phosphate buffer solution, constant temperature oscillation case is placed in 15 DEG C, 300r/min speed oscillations reaction 16
Hour, centrifuged after taking-up, be washed to washing lotion it is limpid it is colourless untill, remove supernatant, then pellet frozen dried, produce immobilization
Complex enzyme；
(3) into the edible mushroom feed liquid, addition accounts for the immobilization complex enzyme of the edible mushroom feed liquid quality 0.3%, in pH
For 6.5, temperature is lasting stirring enzymolysis 2 hours under conditions of 50 DEG C；At the same time, ultrasonic wave-micro- is carried out in enzymolysis process
Ripple collaboration is aided in, and ultrasound condition is：Supersonic frequency 40kHz, power are 500W, and ultrasonic treatment time is 1min；Microwave condition
For：Microwave power 350W, microwave time are 1min；
(4) enzymolysis liquid obtained by step (3) handle into 15min progress destroy the enzyme treatments and by edible mushroom enzyme in 100 DEG C of water-baths
Solution liquid, which be spray-dried, obtains edible mushroom enzymolysis powder, and spray drying condition is：Feeding temperature is 120 DEG C, and leaving air temp is 85
DEG C, feeding speed is 20mL/min.
Embodiment 5
The preparation method of domestic fungus flour described in the present embodiment is as follows：Weigh gained edible mushroom enzymolysis in 100g embodiments 1
Powder, mixes with 900g wheat flours, produces.
Embodiment 6
The preparation method of domestic fungus flour described in the present embodiment is as follows：Weigh gained edible mushroom enzymolysis in 50g embodiments 2
Powder, mixes with 950g wheat flours, produces.
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of edible mushroom enzymolysis process, it is characterised in that comprise the following steps：
(1) pretreatment of raw material：Take dry edible mushroom to crush, obtain powder of edible fungus, and the powder of edible fungus is added water food is made
Bacterium feed liquid is used, it is standby；
(2) preparation of immobilization complex enzyme：Take inorganic carrier to add activator and carry out crosslinking activation processing, and add cellulase
Mixed with protease, reaction is fixed to neutrality in regulation reaction solution pH, and separation of solid and liquid takes precipitation drying, produces the immobilization
Complex enzyme, it is standby；
(3) the immobilization complex enzyme is added into the edible mushroom feed liquid to mix, and is carried out enzyme digestion reaction in 37-60 DEG C, is obtained
Edible mushroom enzymolysis liquid；
(4) enzymolysis liquid obtained by step (3) is subjected to destroy the enzyme treatment, and the enzymolysis liquid is spray-dried, that is, needed for obtaining
Edible mushroom digests powder.
2. edible mushroom enzymolysis process according to claim 1, it is characterised in that in the step (2), the activator is
Glutaraldehyde solution.
3. edible mushroom enzymolysis process according to claim 1 or 2, it is characterised in that described inorganic in the step (2)
Carrier is magnetic hydrotalcite or magnetic Fe3O4Nano particle.
4. the edible mushroom enzymolysis process according to claim any one of 1-3, it is characterised in that the addition of the protease
The 0.02%-0.2wt% of the edible mushroom feed liquid is accounted for, the addition of the cellulase accounts for the edible mushroom feed liquid
0.02%-0.2wt%.
5. the edible mushroom enzymolysis process according to claim any one of 1-4, it is characterised in that described in the step (3)
Enzymolysis step is carried out under ultrasonic synergistic microwave radiation technology；
The frequency of the ultrasonication is 40-60kHz, and power is 150-500W, and ultrasonic treatment time is 1min-10min；
The power of the microwave treatment is 150-350W, and microwave treatment time is 1-10min.
6. the edible mushroom enzymolysis process according to claim any one of 1-5, it is characterised in that described in the step (3)
The addition of immobilization complex enzyme accounts for the 0.1-0.5wt% of the edible mushroom feed liquid quality.
7. the edible mushroom enzymolysis process according to claim any one of 1-6, it is characterised in that described in the step (1)
Powder of edible fungus is according to 1：10-1：The edible mushroom feed liquid is made in 40 solid-liquid ratio, and adjusts the edible mushroom material liquid pH for 6-7.
8. the edible mushroom enzymolysis powder as made from claim 1-7 any described edible mushroom enzymolysis process.
9. the edible mushroom enzymolysis powder described in claim 8 is used for the purposes for preparing domestic fungus flour.
10. a kind of domestic fungus flour, it is characterised in that including：
Edible mushroom enzymolysis powder 0.5%-20wt% described in claim 9；
Wheat flour 80-99.5wt%.
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Cited By (6)
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| CN108618084A (en) * | 2018-05-09 | 2018-10-09 | 仲景食品股份有限公司 | A kind of mushroom enzymolysis powder and preparation method thereof |
| CN109662301A (en) * | 2019-01-28 | 2019-04-23 | 内蒙古红太阳食品有限公司 | A kind of prairie Tricholomataceae bacterium soup of fire pot material and production method |
| CN110786445A (en) * | 2019-09-26 | 2020-02-14 | 黄河三角洲京博化工研究院有限公司 | Preparation process of flavored selenium-rich edible fungus functional flour |
| CN111982629A (en) * | 2020-08-25 | 2020-11-24 | 北京市农林科学院 | Ultrathin freezing slicing method for edible mushroom tissue cells |
| CN114287506A (en) * | 2021-11-05 | 2022-04-08 | 安徽中志科技有限公司 | Preparation method of easy-to-absorb edible fungus protein powder with high organic selenium content |
| NL2031945B1 (en) * | 2021-07-14 | 2022-12-13 | Univ Jiangnan | Fully soluble edible fungus protein and preparation method thereof |
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