CN101011144A - Process for preparing shiitake mushroom extract - Google Patents
Process for preparing shiitake mushroom extract Download PDFInfo
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Abstract
The invention relates to a method for producing mushroom, which comprises that breaking mushroom and adding water, adding common salt to dissolve for 2-20h, adding some prolease to process enzymolysis, disinfecting enzyme and separating the filter solution; adding 5'-phosphodiesterase into filter solution to process the enzymolysis, disinfecting the enzyme and separating to obtain the filter solution; concentrating the filter solution into paste or atomizing and drying into powder. The invention processes two times of enzymolysis, while the product is abundant in amino acid, polypeptide, polysaccharide, or the like. And the invention has high solubility and stability in neutral or weak-alkali water solution, and some solubility in weak-acid water solution.
Description
Technical field
The present invention relates to a kind of mushroom deep process, be specially a kind of production technology of shiitake mushroom extract.
Background technology
Mushroom is the famous edible mushroom of China, at the title of among the people have " mountain delicacy ".Also expensive edible one of medicinal bacterium of holding concurrently of generic name contains protein and 17 seed amino acids, unrighted acid and multivitamin in the world, and especially provitamin D (ergosterol) content is abundant.Contain in addition that lentinan (lentinan) is called for short LNT, mushroom is given birth to very much (lentysin), mushroom adenine (eriadenine) and derivative thereof.The not only bright delicacy incense of mushroom, nutritious, and can regulate metabolism, and build up health, be described as " wholesome food ", " modern food " by people, developing into the 3rd based food---the fungus food outside the plant of continuing, the animal food at present.In order to utilize flavor substance and nutrition and the functional materials in the natural fresh mushroom fully, must carry out deep processing to mushroom and extract processing.In existing deep processing mushroom technology, a part of technology mainly is the report to the extraction process of single component in the mushroom, as mushroom SOD extract, the extraction of polysaccharide etc.The resulting product of this extracting method is more single, and it utilizes direction mainly is to embody medical value.Another part mainly be with edible be the technology of main target.The former specification requirement height, equipment investment is big, and large-scale production acquires a certain degree of difficulty, and is not suitable for practicing midwifery of medium-sized and small enterprises.The latter is based on edible, though production technology is simple, local flavor and functional materials content are lower in the product, and its application is affected.
Summary of the invention
The present invention is single and concentration is low, the problem of extraction effect difference and a kind of production technology of shiitake mushroom extract is provided in order to solve contained nutriment in the Shitake Mushroom P.E that exists in the prior art.
The present invention is realized that by following technical scheme a kind of production technology of shiitake mushroom extract may further comprise the steps:
1, it is broken and to add weight ratio be 1: 3～14 water to get mushroom,
2, adding 1～5% salt (NaCl) of mushroom quality and to adjust PH be 4～8, is self-dissolving 2～20 hours under 40～65 ℃ of conditions in temperature,
3,0.2～1.0% the protease that adds the mushroom quality, in PH=4.5～7.5, temperature is an enzymolysis 1～6 hour under 45～75 ℃ the condition,
4, go out to separate behind the enzyme and obtain filtrate, separation can be taked method centrifugal or that filter;
5, the 5 '-phosphodiesterase enzyme liquid that adds the 1.25-5.00% of filtrate volume in filtrate is under 45-75 ℃ the condition enzymolysis 1-5 hour in the PH=5.0-8.0 temperature,
6, go out to separate behind the enzyme and obtain filtrate;
7, to filtrate at 0.05～0.10MPa, vacuum is concentrated into paste or spray-drying powder process under 50～70 ℃ of conditions.
Prioritization scheme is: a kind of production technology of shiitake mushroom extract may further comprise the steps:
1, it is broken and to add weight ratio be 1: 5～12 water to get mushroom,
2, adding 2～4% salt (NaCl) of mushroom quality and to adjust PH be 5～6, is self-dissolving 2～14 hours under 45～55 ℃ of conditions in temperature,
3,0.4～0.8% the protease that adds the mushroom quality, in PH=5.5～6.5, temperature is an enzymolysis 2～5 hours under 55～65 ℃ the condition,
4, go out to separate behind the enzyme and obtain filtrate, separation can be taked method centrifugal or that filter;
5, the 5 '-phosphodiesterase enzyme liquid that adds filtrate volume 2～3% in filtrate is enzymolysis 1～3 hour under 55-65 ℃ the condition in the PH=5.0-8.0 temperature,
6, the enzyme that goes out, back separate and obtaining filtrate;
7, to filtrate at 0.07～0.09MPa, vacuum is concentrated into paste or spray-drying powder process under 55～65 ℃ of conditions.
The parameter condition is definite in technology of the present invention:
1, material-water ratio is to the influence of mushroom self-dissolving
Self-dissolving extracting condition: pH=7, NaCl addition 2%, 55 ℃ of following self-dissolving 12h measure extract amino nitrogen content and soluble solid content after the enzyme that goes out is centrifugal.Different material-water ratios the results are shown in Figure 1 to the extract amino nitrogen yield of mushroom self-dissolving and the influence of all shape things of solubility yield
As shown in Figure 1, solid-liquid ratio is very big to the influence of product, and material-water ratio is that 1: 5 o'clock amino nitrogen yield is higher, and what the soluble solid yield was the highest is material-water ratio 1: 12, but the amino nitrogen yield of this moment is lower, illustrates that the rare more amino nitrogen yield of mushroom liquid is low more; Increase with hourly water consumption, energy consumption was many when product concentrated, and can increase product cost, considered nutrition and cost, and it is best adopting material-water ratio at 1: 5.
2, temperature is to the influence of mushroom self-dissolving
Than self-dissolving 12h under 1: 5, the condition of pH7, NaCl addition 2%, after the enzyme that goes out is centrifugal, measure extract amino nitrogen content and soluble solid content at water.Temperature influences result such as Fig. 2 to the mushroom self-dissolving
It mainly is to utilize the effect of the enzyme of mushroom system own body internal protein, nucleic acid and glucide etc. to be decomposed into small-molecule substances such as amino acid, peptide class, nucleotides and lentinan that autolysis method is produced shiitake mushroom extract, and temperature is very big to the activity influence of enzyme.If temperature is too high, can make enzyme deactivation, simultaneous temperature is crossed to hang down and is unfavorable for that again enzyme plays a role, and causes the decomposition of protein not thorough, and the ammonia nitrogen yield descends.Take all factors into consideration, better from 55 ℃ of solubility temperatures.
3, pH is to the influence of mushroom self-dissolving
Self-dissolving 12h under the condition of 1: 5,55 ℃ of material-water ratios, interpolation 2%NaCl, different pH the results are shown in Figure 3 to the extract amino nitrogen yield of mushroom self-dissolving and the influence of soluble solids yield
The pH value directly influences the many enzymes system in the mushroom fruiting body, the complete decomposition that this influence is determining content whether, so must suitable pH value of screening.By among the figure as can be known, be amino nitrogen yield or soluble solid yield when pH6 all than higher, so it is more suitable to choose pH6.
4, autolysis promoter NaCl addition is to the influence of mushroom self-dissolving
Under material-water ratio 1: 5, pH6,55 ℃, choose the mushroom self-dissolving that different NaCl additions carries out 12h, measure extract amino nitrogen content and soluble solid content.The influence of extract amino nitrogen yield and soluble solid yield the results are shown in Figure 4.
NaCl can activate a series of hydrolases in the mushroom somatic cells as promoter, promotes self-dissolving, thereby shortens the self-dissolving time, and has antisepsis.In addition, NaCl also has the seasoning effect, can strengthen delicate flavour and regulate mouthfeel.Analyze from figure that to draw 2% addition better.
5, the time is to the influence of mushroom self-dissolving
Material-water ratio 1: 5, pH6,55 ℃, add under the condition of 2%NaCl, behind the self-dissolving different time, measure extract amino nitrogen content and soluble solid content.The influence of time to the mushroom self-dissolving the results are shown in Figure 5.
As shown in the figure, the amino nitrogen yield has decline slightly between the 7-10h, along with self-dissolving time lengthening to 13h amino nitrogen yield obviously improves and reaches the highest, afterwards, along with the prolongation amino nitrogen yield of self-dissolving time descends; The soluble solid yield is the highest when 13h, then slowly raises again along with time lengthening has decline slightly.The self-dissolving time is too short, and self-dissolving is not thorough; But overlong time, extract content improves slowly, has prolonged the production time, has increased production cost.The more important thing is, overlong time, some flavour nucleotides can further be hydrolyzed into tasteless material under the effect of nucleotidase, thereby have reduced the delicate flavour of autolysate.So it is better that the self-dissolving time is controlled at 13h.
6, the orthogonal test of self-dissolving lixiviate optimization
By single factor experiment draft A (time), B (material-water ratio), C (temperature), D (pH) four factors are respectively got three levels, see Table 2, according to orthogonal table L
9(3
4) experimentize, autolysis promoter NaCl addition is 2% (w/w), is index with the ammonia nitrogen yield, determines the optimum condition of mushroom self-dissolving.
Table 1 mushroom self-dissolving condition orthogonal test L
9(3
4) factor level
Table1 Levels of factors for Lentinus autolysis L
9(3
4)
| Factor level | A (time/h) | B (material-water ratio) | C (temperature/℃) | D(pH) |
| 1 | 10 | 1∶3 | 50 | 5 |
| 2 | 13 | 1∶5 | 55 | 6 |
| 3 | 16 | 1∶7 | 60 | 7 |
Table 2 orthogonal experiments
Table2 Orthogonal test and results
| Level | Factor | Index | ||||
| A | B | C | D | Soluble solids yield % | Amino nitrogen yield % | |
| 1 | 1 | 1 | 1 | 1 | 6.42 | 0.16 |
| 2 | 1 | 2 | 2 | 2 | 6.22 | 0.17 |
| 3 | 1 | 3 | 3 | 3 | 5.52 | 0.12 |
| 4 | 2 | 1 | 2 | 3 | 4.29 | 0.13 |
| 5 | 2 | 2 | 3 | 1 | 6.39 | 0.18 |
| 6 | 2 | 3 | 1 | 2 | 7.53 | 0.13 |
| 7 | 3 | 1 | 3 | 2 | 2.88 | 0.10 |
| 7 | 3 | 2 | 1 | 3 | 6.09 | 0.13 |
| 9 | 3 | 3 | 2 | 1 | 6.80 | 0.09 |
| K 1 | 0.45 | 0.39 | 0.42 | 0.43 | ||
| K 2 | 0.44 | 0.48 | 0.39 | 0.40 | ||
| K 3 | 0.32 | 0.34 | 0.40 | 0.38 | ||
| k 1 | 0.15 | 0.13 | 0.14 | 0.143 | ||
| k 2 | 0.147 | 0.16 | 0.13 | 0.133 | ||
| k 3 | 0.11 | 0.11 | 0.133 | 0.127 | ||
| R | 0.04 | 0.05 | 0.01 | 0.016 |
By the interpretation of result of table 2 extreme difference as can be known, the variation of the level of factor to amino nitrogen yield effect size is: B＞A＞D＞C, i.e. material-water ratio＞time＞pH＞temperature.The combination of mushroom self-dissolving lixiviate optimal conditions is A
1B
2C
1D
1, promptly material-water ratio is 1: 5, time 10h, 50 ℃ of temperature, pH=5.
Conclusion
Best self-dissolving extracting technology parameter is: material-water ratio 1: 5, time 10h, 50 ℃ of temperature, pH=5, NaCL addition 2%.
Add the optimizing process of protease to mushroom self-dissolving process conditions
Pretreatment of raw material is the same, produces material-water ratio and be 1: 5 mushroom liquid, and is standby.
2.1 the external enzyme amount is to the influence of mushroom self-dissolving
Add 0.2%, 0.4%, 0.6%, 0.8%, 1.0%, 1.2%, 1.4% protease respectively, at pH6,50 ℃ of following self-dissolving 7h, after the enzyme that goes out is centrifugal, measure extract amino nitrogen content, soluble solid content, lentinan content, determine suitable enzyme concentration.
As shown in Figure 6, along with the increase of protease addition, amino nitrogen yield and soluble solid yield also constantly increase, but after enzyme concentration surpassed 0.6%, the recruitment of yield and yield obviously reduced, and descend to some extent.Thereby the addition of protease is 0.6%.
2.2 temperature is to the influence of the enzyme-added self-dissolving of mushroom
The protease of transferring pH6, adding 0.6% is respectively at 45 ℃ of temperature, 50 ℃, 55 ℃, 60 ℃, 65 ℃, 70 ℃ following self-dissolving 7h, and the mensuration project is the same after the enzyme that goes out is centrifugal, determines suitable temperature.
In the enzyme-added self-dissolving process of mushroom, add the protease acting in conjunction degrade proteins in protease and the mushroom somatic cells.As shown in Figure 7, temperature is bigger to the influence of the amino nitrogen yield of enzyme-added self-dissolving and soluble solid yield.When temperature was 55 ℃, the amino nitrogen yield and the soluble solid yield of mushroom extract all reached maximum, thereby 55 ℃ is the optimum temperature of enzyme-added self-dissolving.
2.3pH influence to the enzyme-added self-dissolving of mushroom
Transfer pH4.5,5.0,5.5,6.0,6.5,7.0,7.5 respectively, the protease of adding 0.6% is at 55 ℃ of following self-dissolving 7h, and the mensuration project is the same after the enzyme that goes out is centrifugal, determines suitable pH.
In the enzyme-added self-dissolving process of mushroom, the variation of pH is very big to the amino nitrogen yield influence of extract, and less relatively to the influence of solubility thing yield, during pH5.0, amino nitrogen yield and yield all reach maximum, thereby pH5.0 is the optimum pH of enzyme-added self-dissolving.
2.4 the enzyme-added time is to the influence of the enzyme-added self-dissolving of mushroom
Add 0.6% protease respectively under pH5.0,55 ℃, continue the self-dissolving certain hour behind self-dissolving 0,1,2,3,4,5,6h, the mensuration project is the same through the enzyme that goes out is centrifugal after, determines the enzyme-added time that suits.
As seen from Figure 9, amino nitrogen yield and the soluble solid yield of enzyme-added time for the mushroom extract all has certain influence, direct enzyme-added self-dissolving is enzyme-added during this behind respectively self-dissolving 1, the 2h, the amino nitrogen yield improves along with time lengthening, the yield maximum behind the self-dissolving 2h after the enzyme-added processing; Again every the enzyme-added self-dissolving of 1h, yield reduces gradually afterwards.Simultaneously, enzyme-added soluble solid yield also is maximum behind self-dissolving 2h, thereby enzyme-added effect is best behind the self-dissolving 2h.
2.5 the self-dissolving time is to the influence of the enzyme-added self-dissolving of mushroom
Behind pH5.0,55 ℃ of following self-dissolving 2h, add 0.6% protease and continue self-dissolving, after the centrifugal back of the enzyme that goes out mensuration project is the same, determine the suitable self-dissolving time every 2h.
As shown in Figure 10, when the self-dissolving time surpassed 7h, yield and yield slightly descended and are milder, thereby 7h is the best self-dissolving time.
2.6 conclusion
Through experiment of single factor research, add the optimization result of protease: enzyme concentration 0.6%, 55 ℃ of temperature, pH5.0, enzyme-added time 2h, self-dissolving time 7h to mushroom self-dissolving process conditions.
Compared with prior art, the product that obtains of twice zymolysis technique of this technology utilization is the natural flavouring that a kind of nutrition and function have both.Product is brown paste, is rich in several amino acids, polypeptide, flavour nucleotide and lentinan, has unique strong natural mushroom local flavor.This product solubility is good, and is easily molten in the water more than 50 ℃, stable in neutrality or weak alkaline aqueous solution, more stable in weakly acidic solution, can be used as functional component and flavor additives useful in food, flavoring and the flavouring processing.
The resulting shiitake mushroom extract of this production method (water content is 68.7%) yield reaches 21%, improves 13.27% than common process shiitake mushroom extract yield; Shiitake mushroom extract amino nitrogen content 1.19mg/ml improves 6.25% than common process amino nitrogen content; Shiitake mushroom extract 5 '-nucleotide content is 0.0353mg/ml, improves 4.53% when not using 5 '-phosphodiesterase; Lentinan content 0.2496mg/g improves 5.1% than common process lentinan content.The material that obtains by the present invention can be widely used in the food such as baste, soy sauce, instant food, spiced and stewed food, nutrient and healthcare products, cake.
Description of drawings
Fig. 1 is the influence of material-water ratio to the mushroom self-dissolving
Fig. 2 is the influence of temperature to the mushroom self-dissolving
Fig. 3 is the influence of pH to the mushroom self-dissolving
Fig. 4 is the influence of autolysis promoter NaCl addition to the mushroom self-dissolving
Fig. 5 is the influence of time to the mushroom self-dissolving
Fig. 6 is the influence of external enzyme amount to the mushroom self-dissolving
Fig. 7 is the influence of temperature to the enzyme-added self-dissolving of mushroom
Fig. 8 is the influence of pH to the enzyme-added self-dissolving of mushroom
Fig. 9 is the influence of enzyme-added time to the enzyme-added self-dissolving of mushroom
Figure 10 is the influence of self-dissolving time to the enzyme-added self-dissolving of mushroom
The specific embodiment
Embodiment 1: a kind of production technology of shiitake mushroom extract may further comprise the steps:
1, it is broken and to add weight ratio be 1: 3 water to get mushroom,
2, adding 1% salt (NaCl) of mushroom quality and to adjust PH be 4, is self-dissolving 8 hours under 40 ℃ of conditions in temperature, adjusts PH and can adopt acetate or hydrochloric acid and sodium hydroxide solution adjusting.
3,0.2% the protease that adds the mushroom quality, at PH=4.5, temperature is an enzymolysis 1 hour under 45 ℃ the condition, protease is selected papain or microbial protease for use;
4, go out to separate behind the enzyme and obtain filtrate, separation can take method centrifugal or that filter to remove the residue of mushroom, obtains clear liquid; Generally heating the enzyme that went out in 15 minutes down at 95 ℃ handles;
5, the 5 '-phosphodiesterase enzyme liquid that adds filtrate volume 1.25% in filtrate is enzymolysis 1 hour under 65 ℃ the condition in PH=6.5, temperature,
6, the enzyme that goes out, back separate and obtaining filtrate; Generally heating the enzyme that went out in 10 minutes down at 95 ℃ handles;
7, to filtrate at 0.05MPa, vacuum is concentrated into paste or spray-drying powder process under 50 ℃ of conditions.
Embodiment 2: a kind of production technology of shiitake mushroom extract may further comprise the steps:
1, it is broken and to add weight ratio be 1: 14 water to get mushroom,
2, adding 5% salt (NaCl) of mushroom quality and to adjust PH be 8, is self-dissolving 20 hours under 65 ℃ of conditions in temperature,
3,1.0% the protease that adds the mushroom quality, at PH=7.5, temperature is an enzymolysis 6 hours under 75 ℃ the condition, protease is selected papain or microbial protease for use;
4, go out to separate behind the enzyme and obtain filtrate, separation can take method centrifugal or that filter to remove the residue of mushroom, obtains clear liquid; Generally heating the enzyme that went out in 15 minutes down at 95 ℃ handles;
5, the 5 '-phosphodiesterase enzyme liquid that adds filtrate volume 4.25% in filtrate is enzymolysis 5 hours under 45 ℃ the condition in PH=5.5, temperature,
6, go out to separate behind the enzyme and obtain filtrate; Generally heating the enzyme that went out in 10 minutes down at 95 ℃ handles;
7, to filtrate at 0.10MPa, vacuum is concentrated into paste or spray-drying powder process under 70 ℃ of conditions.
Embodiment 3: a kind of production technology of shiitake mushroom extract may further comprise the steps:
1, it is broken and to add weight ratio be 1: 5 water to get mushroom.Get fresh mushroom (water content 89.6%) through cleaning, drench and do, broken back adds the water of 2 times of fresh mushroom weight, makes the homogenate shape with beater, adds the water of 3 times of fresh mushroom weight again.2, adding 2% salt (NaCl) of mushroom quality and to adjust PH be 5, is self-dissolving 14 hours under 45 ℃ of conditions in temperature,
3,0.4% the protease that adds the mushroom quality, at PH=5.5, temperature is an enzymolysis 2 hours under 55 ℃ the condition, protease is selected papain or microbial protease for use;
4, go out to separate behind the enzyme and obtain filtrate, separation can take method centrifugal or that filter to remove the residue of mushroom, obtains clear liquid; Generally heating the enzyme that went out in 15 minutes down at 95 ℃ handles;
5, the 5 '-phosphodiesterase enzyme liquid that adds filtrate volume 3.75% in filtrate is enzymolysis 4 hours under 60 ℃ the condition in the PH=8 temperature,
6, the enzyme that goes out, back separate and obtaining filtrate; Generally heating the enzyme that went out in 10 minutes down at 95 ℃ handles;
7, to filtrate at 0.07MPa, vacuum is concentrated into paste or spray-drying powder process under 55 ℃ of conditions.
Embodiment 4: a kind of production technology of shiitake mushroom extract may further comprise the steps:
1, it is broken and to add weight ratio be 1: 12 water to get mushroom, and 2, to add 4% salt (NaCl) of mushroom quality and adjust PH be 6, be self-dissolving 8 hours under 55 ℃ of conditions in temperature,
3,0.8% the protease that adds the mushroom quality, at PH=6.5, temperature is an enzymolysis 5 hours under 65 ℃ the condition, protease is selected papain or microbial protease for use;
4, go out to separate behind the enzyme and obtain filtrate, separation can take method centrifugal or that filter to remove the residue of mushroom, obtains clear liquid; Generally heating the enzyme that went out in 15 minutes down at 95 ℃ handles;
5, the 5 '-phosphodiesterase enzyme liquid that adds filtrate volume 2% in filtrate is enzymolysis 3 hours under 50 ℃ the condition in PH=6, temperature,
6, the enzyme that goes out, back separate and obtaining filtrate; Generally heating the enzyme that went out in 10 minutes down at 95 ℃ handles;
7, to filtrate at 0.09MPa, vacuum is concentrated into paste or spray-drying powder process under 65 ℃ of conditions.
Embodiment 5: a kind of production technology of shiitake mushroom extract may further comprise the steps:
1, it is broken and to add weight ratio be 1: 5 water to get mushroom, and 2, to add 2% salt (NaCl) of mushroom quality and adjust PH be 5, be self-dissolving 2 hours under 51 ℃ of conditions in temperature,
3,0.6% the protease that adds the mushroom quality, at PH=5.0, temperature is an enzymolysis 5 hours under 55 ℃ the condition, protease is selected papain or microbial protease for use;
4, go out to separate behind the enzyme and obtain filtrate, separation can take method centrifugal or that filter to remove the residue of mushroom, obtains clear liquid; Generally heating the enzyme that went out in 15 minutes down at 95 ℃ handles;
5, the 5 '-phosphodiesterase enzyme liquid that adds filtrate volume 2.5% in filtrate is enzymolysis 2 hours under 55 ℃ the condition in PH=7, temperature,
6, go out to separate behind the enzyme and obtain filtrate; Generally heating the enzyme that went out in 10 minutes down at 95 ℃ handles;
7, to filtrate at 0.08MPa, vacuum is concentrated into paste or spray-drying powder process under 65 ℃ of conditions.
Claims (6)
1, a kind of production technology of shiitake mushroom extract is characterized in that: may further comprise the steps:
(1), to get mushroom broken and to add weight ratio be 1: 3～14 water,
(2), to add 1～5% salt (NaCl) of mushroom quality and adjust PH be 4～8, is self-dissolving 2～20 hours under 40～65 ℃ of conditions in temperature,
(3), add 0.2～1.0% protease of mushroom quality, in PH=4.5～7.5, temperature is an enzymolysis 1～6 hour under 45～75 ℃ the condition,
(4), separate behind the enzyme that goes out and obtain filtrate, separation can be taked method centrifugal or that filter;
(5), 5 '-phosphodiesterase enzyme liquid of adding the 1.25-5.00% of filtrate volume in filtrate is under 45～75 ℃ the condition enzymolysis 1-5 hour in the PH=6.0-8.0 temperature,
(6), go out to separate behind the enzyme and obtain filtrate.
2, the production technology of shiitake mushroom extract according to claim 1 is characterized in that: may further comprise the steps:
(1), to get mushroom broken and to add weight ratio be 1: 5～12 water,
(2), to add 2～4% salt (NaCl) of mushroom quality and adjust PH be 5～6, is self-dissolving 2～14 hours under 45～55 ℃ of conditions in temperature,
(3), add 0.4～0.8% protease of mushroom quality, in PH=5.5～6.5, temperature is an enzymolysis 2～5 hours under 55～65 ℃ the condition,
(4), separate behind the enzyme that goes out and obtain filtrate, separation can be taked method centrifugal or that filter;
(5), in filtrate, add 5 '-phosphodiesterase enzyme liquid of filtrate volume 2～3%, be enzymolysis 1～3 hour under 55-65 ℃ the condition in PH=5.0～8.0 temperature,
(6), the enzyme that goes out, the back is separated and to be obtained filtrate.
3, the production technology of shiitake mushroom extract according to claim 1 is characterized in that: may further comprise the steps:
(1), to get mushroom broken and to add weight ratio be 1: 5 water,
(2), to add 2% salt (NaCl) of mushroom quality and adjust PH be 5, is self-dissolving 2 hours under the 50-55 ℃ of condition in temperature,
(3), add 0.6% protease of mushroom quality, at PH=5.0, temperature is an enzymolysis 5 hours under 55 ℃ the condition, protease is selected papain or microbial protease for use;
(4), separate behind the enzyme that goes out and obtain filtrate, separation can take method centrifugal or that filter to remove the residue of mushroom, obtains clear liquid; Generally heating the enzyme that went out in 15 minutes down at 95 ℃ handles;
(5), in filtrate, add 5 '-phosphodiesterase enzyme liquid of filtrate volume 2.5%, be enzymolysis 2 hours under 55 ℃ the condition in PH=7, temperature,
(6), go out to separate behind the enzyme and obtain filtrate; Generally heating the enzyme that went out in 10 minutes down at 95 ℃ handles.
4, according to the production technology of claim 1 or 2 or 3 described shiitake mushroom extracts, it is characterized in that: at 0.05～0.10MPa, vacuum is concentrated into paste or spray-drying powder process under 50～70 ℃ of conditions to filtrate.
5, the production technology of shiitake mushroom extract according to claim 4 is characterized in that: at 0.07～0.09MPa, vacuum is concentrated into paste or spray-drying powder process under 55～65 ℃ of conditions to filtrate.
6, the production technology of shiitake mushroom extract according to claim 5 is characterized in that: at 0.08MPa, vacuum is concentrated into paste or spray-drying powder process under 65 ℃ of conditions to filtrate.
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| CN101263886B (en) * | 2008-04-28 | 2011-03-16 | 广东省微生物研究所 | Preparation of edible mushroom seasoning base |
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| CN116369504A (en) * | 2023-05-22 | 2023-07-04 | 湖北省农业科学院农产品加工与核农技术研究所 | Lentinus edodes extract rich in lentinula edodes purine, lentinula edodes residue proteolytic peptide liquid, and preparation method and application thereof |
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| CN101836706B (en) * | 2010-04-29 | 2013-04-03 | 李洁 | Method for preparing mushroom noodles |
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