CN108617403A - A kind of Se-rich xianggu production technology - Google Patents
A kind of Se-rich xianggu production technology Download PDFInfo
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- CN108617403A CN108617403A CN201810454063.2A CN201810454063A CN108617403A CN 108617403 A CN108617403 A CN 108617403A CN 201810454063 A CN201810454063 A CN 201810454063A CN 108617403 A CN108617403 A CN 108617403A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
Abstract
The invention discloses a kind of Se-rich xianggu production technologies, include the following steps：It is prepared by level-one parent species：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put agar, glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B are added after agar dissolves1, sodium selenite and water, 1000 milliliters of culture mediums are made, test tube high pressure sterilization is dispensed, when pressure reaches 0.05MPa, lets cool air back to zero, it is pressurized to 0.14MPa and maintains 30 minutes back to zeros, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, inclined-plane is covered with through being cultivated 15 days or so under 25 DEG C of constant temperature, obtains level-one kind.The yield and quality of the present invention improves 10% or more than common mushroom, the ratio of first-grade products increases by 20%, Se content per kilogram mushroom is up to 1.6 milligrams, increase by 160 times compared with general mushroom, solves the problem of yield and quality that existing Se-rich xianggu planting type is easy to be caused to review mushroom by living contaminants in the course of cultivation reduces.
Description
Technical field
The present invention relates to mushroom Cultivating techniques field, specially a kind of Se-rich xianggu production technology.
Background technology
Selenium (Se) is the high-efficient oxidant in human body, has and improves a variety of biologies such as immunity of organisms, antitumor, anti-aging
Learn function.1973, WHO and international nutritive issue confirmed it for required trace element in human body and animal body.But full generation
Boundary has nearly 50 countries and regions to have the problem of selenium deficiency, the problem can be made to be resolved by producing Se-rich farm products.
Early in 1984, Finland just utilized the fertilizer production cereal containing sodium selenate and successful.The beginning of the nineties, China various regions also land
It is continuous to start Se-rich farm products production.Se-rich farm products production as a result, experienced one from inefficient dispersal to the process efficiently concentrated.
China's Se-rich farm products have begun to take shape at present, mainly there is more than 30 a kind such as selenium-enriched edible mushroom, selenium-enriched rice, selenium-enriched tea leaf.
Wherein, the development of selenium-enriched edible mushroom has clear superiority.
Currently, having carried out selenium-enriching cultivation research and production to multiple eating bacterium both at home and abroad, such as：Needle mushroom, oyster mushroom, spirit
Sesame, Hericium erinaceus, agaric, mushroom, agrocybe, elegant precious mushroom, coprinus comatus etc..Since different edible mushrooms are to the tolerance of selenium and enrichment power
It is different and differ greatly, so the addition of selenium is usually carried out according to the characteristic of kind in production.By considering adding for selenium
Add mode, the mode of production, additive amount, thalline (silk) yield, enrichment power, the rate of recovery, production cost and safety, it is specific to determine
The mode of production of mushroom kind, existing Se-rich xianggu planting type are easy, by living contaminants, to cause to review mushroom in the course of cultivation
Yield and quality reduce, for this purpose, it is proposed that a kind of Se-rich xianggu production technology.
Invention content
The purpose of the present invention is to provide a kind of Se-rich xianggu production technologies, to solve mentioned above in the background art ask
Topic.
To achieve the above object, the present invention provides the following technical solutions：A kind of Se-rich xianggu production technology, including walk as follows
Suddenly：
(1) prepared by level-one parent species：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put agar, wait for
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added after dissolving in agar1, sodium selenite and water, be made 1000
Milliliter culture medium, dispenses test tube high pressure sterilization, when pressure reaches 0.05MPa, lets cool air back to zero, is pressurized to 0.14MPa maintenances
30 minutes back to zeros, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through under 25 DEG C of constant temperature
Culture covers with inclined-plane in 15 days or so, obtains level-one kind；
(2) prepared by two level parent species：Mix packing seed bottle thoroughly, tampon sealing sterilizing, it is zero that pressure cools down in 0.05MPa,
Pressurization continues 3 hours when rising to 0.14MPa after back to zero, and temperature is down to 30 DEG C or less aseptic conditions access level-one parent species, every one
Grade parent species 5-8 bottles of original seeds of switching, cultivate 40 days or so in 25 DEG C of dark surrounds and obtain two level kind；
(3) prepared by three-level parent species：Cultigen is produced with polyethylene bagging, and normal-pressure sterilization maintains 48 again when temperature is up to 100 DEG C
Hour, when the back pkt. that takes the dish out of the pot is cooled to 30 DEG C or less, two level kind is added under aseptic condition, three-level kind is obtained by culture；
(4) Se-rich xianggu is cultivated：It adds liquid organic selenium fertilizer again in medium culture material, is produced with polyethylene bagging,
Normal-pressure sterilization, temperature continue 48 hours up to 100 DEG C, and temperature is down to 30 DEG C hereinafter, accessing three-level kind i.e. under aseptic condition after taking the dish out of the pot
To produce bag.
Preferably, potato 200g, glucose 20g, agar 20-25g, peptone 1-3g, phosphatide two in the step (1)
Hydrogen potassium 0.5-1.5g, magnesium sulfate 0.5-1.5g, vitamin B10.5-1.5g, appropriate sodium selenite, 1000 milliliters of water.
Preferably, raw material is chosen by weight proportion in the step (2)：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, stone
Cream 1%, appropriate sodium selenite, material water weight ratio are 1:1.2.
Preferably, raw material is chosen by weight proportion in the step (3)：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, stone
Cream 1%, appropriate sodium selenite, material water weight ratio are 1:1.2.
Preferably, raw material is chosen by weight proportion in the step (4)：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, stone
Cream 1%, appropriate sodium selenite, per kilogram compost add 1.5-1.6 milliliters of organic selenium fertilizer, material-water ratio example to be 1:1.2.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention introduces strain first, passes through sub kind of entity separation
It carries out turning to expand it is preferred that going out excellent species, produces parent species, original seed and the cultigen for being suitble to cultivation, by the accumulation ability of mushroom,
Strains at different levels are tamed, the best selenium amount of application of mushroom is preferably gone out, then the liquid sodium selenite and organic selenium fertilizer that will have been configured
It is added in culture material, bacterial contamination rate can be greatly reduced, yield and quality improves 10% or more than common mushroom, and the ratio of first-grade products increases
Add 20%, Se content per kilogram mushroom increases by 160 times compared with general mushroom, solve existing Se-rich xianggu up to 1.6 milligrams
The problem of yield and quality that planting type is easy to be caused to review mushroom by living contaminants in the course of cultivation reduces.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
The every other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of technical solution：A kind of Se-rich xianggu production technology, includes the following steps：
(1) prepared by level-one parent species：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put agar, wait for
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added after dissolving in agar1, sodium selenite and water, be made 1000
Milliliter culture medium, dispenses test tube high pressure sterilization, when pressure reaches 0.05MPa, lets cool air back to zero, is pressurized to 0.14MPa maintenances
30 minutes back to zeros, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through under 25 DEG C of constant temperature
Culture covers with inclined-plane in 15 days or so, obtains level-one kind；
(2) prepared by two level parent species：Mix packing seed bottle thoroughly, tampon sealing sterilizing, it is zero that pressure cools down in 0.05MPa,
Pressurization continues 3 hours when rising to 0.14MPa after back to zero, and temperature is down to 30 DEG C or less aseptic conditions access level-one parent species, every one
Grade parent species 5-8 bottles of original seeds of switching, cultivate 40 days or so in 25 DEG C of dark surrounds and obtain two level kind；
(3) prepared by three-level parent species：Cultigen is produced with polyethylene bagging, and normal-pressure sterilization maintains 48 again when temperature is up to 100 DEG C
Hour, when the back pkt. that takes the dish out of the pot is cooled to 30 DEG C or less, two level kind is added under aseptic condition, three-level kind is obtained by culture；
(4) Se-rich xianggu is cultivated：It adds liquid organic selenium fertilizer again in medium culture material, is produced with polyethylene bagging,
Normal-pressure sterilization, temperature continue 48 hours up to 100 DEG C, and temperature is down to 30 DEG C hereinafter, accessing three-level kind i.e. under aseptic condition after taking the dish out of the pot
To produce bag.
Embodiment one：
Level-one parent species preparation is carried out first：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put fine jade
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added in fat after agar dissolves1, sodium selenite and water, be made
1000 milliliters of culture mediums dispense test tube high pressure sterilization, when pressure reaches 0.05MPa, let cool air back to zero, are pressurized to 0.14MPa
30 minutes back to zeros are maintained, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through 25 DEG C of perseverances
The lower culture of temperature covers with inclined-plane in 15 days or so, obtains level-one kind；Then two level parent species preparation is carried out：Mix packing seed bottle, tampon thoroughly
Sealing sterilizing, it is zero that pressure cools down in 0.05MPa, when pressurization rises to 0.14MPa after back to zero, continues 3 hours, temperature is down to 30
DEG C or less aseptic condition access level-one parent species, every level-one parent species are transferred 5-8 bottles of original seeds, and 40 are cultivated in 25 DEG C of dark surrounds
It or so obtains two level kind；Then three-level parent species preparation is carried out：Cultigen is produced with polyethylene bagging, and normal-pressure sterilization, temperature reaches
It is maintained again at 100 DEG C 48 hours, when the back pkt. that takes the dish out of the pot is cooled to 30 DEG C or less, two level kind is added under aseptic condition, by cultivating
To three-level kind；Finally carry out Se-rich xianggu cultivation：Liquid organic selenium fertilizer is added again in medium culture material, with polyethylene bagging
Production, normal-pressure sterilization, temperature continue 48 hours up to 100 DEG C, and temperature is down to 30 DEG C hereinafter, accessing three-level under aseptic condition after taking the dish out of the pot
Kind is production bag.
Embodiment two：
In embodiment one, following processes are added：
Potato 200g, glucose 20g, agar 20-25g, peptone 1-3g, phosphatide potassium dihydrogen 0.5- in step (1)
1.5g, magnesium sulfate 0.5-1.5g, vitamin B10.5-1.5g, appropriate sodium selenite, 1000 milliliters of water.
Level-one parent species preparation is carried out first：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put fine jade
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added in fat after agar dissolves1, sodium selenite and water, be made
1000 milliliters of culture mediums dispense test tube high pressure sterilization, when pressure reaches 0.05MPa, let cool air back to zero, are pressurized to 0.14MPa
30 minutes back to zeros are maintained, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through 25 DEG C of perseverances
The lower culture of temperature covers with inclined-plane in 15 days or so, obtains level-one kind, wherein potato 200g, glucose 20g, agar 20-25g, albumen
Peptone 1-3g, phosphatide potassium dihydrogen 0.5-1.5g, magnesium sulfate 0.5-1.5g, vitamin B10.5-1.5g, appropriate sodium selenite, water 1000
Milliliter；Then two level parent species preparation is carried out：Mix packing seed bottle thoroughly, tampon sealing sterilizing, pressure cools down in 0.05MPa is
Zero, when pressurization rises to 0.14MPa after back to zero, continue 3 hours, temperature is down to 30 DEG C or less aseptic conditions access level-one parent species, often
Level-one parent species are transferred 5-8 bottles of original seeds, are cultivated 40 days or so in 25 DEG C of dark surrounds and are obtained two level kind；Then three-level is carried out
It is prepared by parent species：Cultigen is produced with polyethylene bagging, and normal-pressure sterilization maintains 48 hours again when temperature is up to 100 DEG C, the back pkt. that takes the dish out of the pot drop
When temperature is to 30 DEG C or less, two level kind is added under aseptic condition, three-level kind is obtained by culture；Finally carry out Se-rich xianggu cultivation：
It adds liquid organic selenium fertilizer again in medium culture material, is produced with polyethylene bagging, normal-pressure sterilization, temperature continues up to 100 DEG C
48 hours, temperature was down to 30 DEG C hereinafter, it is to produce bag to access three-level kind under aseptic condition after taking the dish out of the pot.
Embodiment three：
In embodiment two, following processes are added：
Step chooses raw material by weight proportion in (2)：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, sub- selenium
Sour sodium is appropriate, material water weight ratio is 1:1.2.
Level-one parent species preparation is carried out first：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put fine jade
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added in fat after agar dissolves1, sodium selenite and water, be made
1000 milliliters of culture mediums dispense test tube high pressure sterilization, when pressure reaches 0.05MPa, let cool air back to zero, are pressurized to 0.14MPa
30 minutes back to zeros are maintained, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through 25 DEG C of perseverances
The lower culture of temperature covers with inclined-plane in 15 days or so, obtains level-one kind, wherein potato 200g, glucose 20g, agar 20-25g, albumen
Peptone 1-3g, phosphatide potassium dihydrogen 0.5-1.5g, magnesium sulfate 0.5-1.5g, vitamin B10.5-1.5g, appropriate sodium selenite, water 1000
Milliliter；Then two level parent species preparation is carried out：Raw material is chosen by weight proportion：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum
1%, appropriate sodium selenite, material water weight ratio are 1:1.2, mix packing seed bottle thoroughly, tampon sealing sterilizing, pressure is in 0.05MPa
Shi Jiangwen is zero, when pressurization rises to 0.14MPa after back to zero, continues 3 hours, and temperature is down to 30 DEG C or less aseptic condition access level-ones
Parent species, every level-one parent species 5-8 bottles of original seeds of switching, cultivate 40 days or so in 25 DEG C of dark surrounds and obtain two level kind；Then
Carry out three-level parent species preparation：Cultigen is produced with polyethylene bagging, and normal-pressure sterilization maintains 48 hours when temperature is up to 100 DEG C, goes out again
When pot back pkt. is cooled to 30 DEG C or less, two level kind is added under aseptic condition, three-level kind is obtained by culture；It is fragrant finally to carry out selenium-rich
Mushroom is cultivated：It adds liquid organic selenium fertilizer again in medium culture material, is produced with polyethylene bagging, normal-pressure sterilization, temperature is up to 100
DEG C continue 48 hours, temperature is down to 30 DEG C hereinafter, it is to produce bag to access three-level kind under aseptic condition after taking the dish out of the pot.
Example IV：
In embodiment three, following processes are added：
Step chooses raw material by weight proportion in (3)：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, sub- selenium
Sour sodium is appropriate, material water weight ratio is 1:1.2.
Level-one parent species preparation is carried out first：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put fine jade
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added in fat after agar dissolves1, sodium selenite and water, be made
1000 milliliters of culture mediums dispense test tube high pressure sterilization, when pressure reaches 0.05MPa, let cool air back to zero, are pressurized to 0.14MPa
30 minutes back to zeros are maintained, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through 25 DEG C of perseverances
The lower culture of temperature covers with inclined-plane in 15 days or so, obtains level-one kind, wherein potato 200g, glucose 20g, agar 20-25g, albumen
Peptone 1-3g, phosphatide potassium dihydrogen 0.5-1.5g, magnesium sulfate 0.5-1.5g, vitamin B10.5-1.5g, appropriate sodium selenite, water 1000
Milliliter；Then two level parent species preparation is carried out：Raw material is chosen by weight proportion：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum
1%, appropriate sodium selenite, material water weight ratio are 1:1.2, mix packing seed bottle thoroughly, tampon sealing sterilizing, pressure is in 0.05MPa
Shi Jiangwen is zero, when pressurization rises to 0.14MPa after back to zero, continues 3 hours, and temperature is down to 30 DEG C or less aseptic condition access level-ones
Parent species, every level-one parent species 5-8 bottles of original seeds of switching, cultivate 40 days or so in 25 DEG C of dark surrounds and obtain two level kind；Then
Carry out three-level parent species preparation：Raw material is chosen by weight proportion：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, sub- selenium
Sour sodium is appropriate, material water weight ratio is 1:1.2, cultigen is produced with polyethylene bagging, and normal-pressure sterilization is tieed up again when temperature is up to 100 DEG C
It holds 48 hours, when the back pkt. that takes the dish out of the pot is cooled to 30 DEG C or less, two level kind is added under aseptic condition, three-level kind is obtained by culture；Most
Se-rich xianggu cultivation is carried out afterwards：It adds liquid organic selenium fertilizer again in medium culture material, is produced with polyethylene bagging, normal pressure goes out
Bacterium, temperature continue 48 hours up to 100 DEG C, and temperature is down to 30 DEG C hereinafter, it is to produce to access three-level kind under aseptic condition after taking the dish out of the pot
Bag.
Embodiment five：
In example IV, following processes are added：
Step chooses raw material by weight proportion in (4)：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, sub- selenium
Sour sodium is appropriate, per kilogram compost adds 1.5-1.6 milliliters of organic selenium fertilizer, and material-water ratio example is 1:1.2.
Level-one parent species preparation is carried out first：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put fine jade
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B is added in fat after agar dissolves1, sodium selenite and water, be made
1000 milliliters of culture mediums dispense test tube high pressure sterilization, when pressure reaches 0.05MPa, let cool air back to zero, are pressurized to 0.14MPa
30 minutes back to zeros are maintained, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through 25 DEG C of perseverances
The lower culture of temperature covers with inclined-plane in 15 days or so, obtains level-one kind, wherein potato 200g, glucose 20g, agar 20-25g, albumen
Peptone 1-3g, phosphatide potassium dihydrogen 0.5-1.5g, magnesium sulfate 0.5-1.5g, vitamin B10.5-1.5g, appropriate sodium selenite, water 1000
Milliliter；Then two level parent species preparation is carried out：Raw material is chosen by weight proportion：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum
1%, appropriate sodium selenite, material water weight ratio are 1:1.2, mix packing seed bottle thoroughly, tampon sealing sterilizing, pressure is in 0.05MPa
Shi Jiangwen is zero, when pressurization rises to 0.14MPa after back to zero, continues 3 hours, and temperature is down to 30 DEG C or less aseptic condition access level-ones
Parent species, every level-one parent species 5-8 bottles of original seeds of switching, cultivate 40 days or so in 25 DEG C of dark surrounds and obtain two level kind；Then
Carry out three-level parent species preparation：Raw material is chosen by weight proportion：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, sub- selenium
Sour sodium is appropriate, material water weight ratio is 1:1.2, cultigen is produced with polyethylene bagging, and normal-pressure sterilization is tieed up again when temperature is up to 100 DEG C
It holds 48 hours, when the back pkt. that takes the dish out of the pot is cooled to 30 DEG C or less, two level kind is added under aseptic condition, three-level kind is obtained by culture；Most
Se-rich xianggu cultivation is carried out afterwards：Raw material is chosen by weight proportion：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, Asia
Appropriate sodium selenate, per kilogram compost add 1.5-1.6 milliliters of organic selenium fertilizer, material-water ratio example to be 1:1.2, in medium culture material
Liquid organic selenium fertilizer is added again, is produced with polyethylene bagging, and normal-pressure sterilization, temperature continues 48 hours up to 100 DEG C, temperature after taking the dish out of the pot
30 DEG C are down to hereinafter, it is to produce bag to access three-level kind under aseptic condition.
The present invention introduces strain first, and preferably going out excellent species by son kind of entity separation carries out turning to expand, and produces suitable
Parent species, original seed and the cultigen of cultivation tame strains at different levels by the accumulation ability of mushroom, and it is best preferably to go out mushroom
Selenium amount of application, then the liquid sodium selenite and organic selenium fertilizer that have configured are added in culture material, bacterial contamination rate can be greatly reduced,
Yield and quality than common mushroom improve 10% or more, the ratio of first-grade products increase by 20%, Se content per kilogram mushroom up to 1.6 milligrams,
Increase by 160 times compared with general mushroom, solves existing Se-rich xianggu planting type and be easy in the course of cultivation by miscellaneous bacteria dirt
The problem of contaminating, the yield and quality for reviewing mushroom caused to reduce.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of Se-rich xianggu production technology, it is characterised in that：Include the following steps：
(1) prepared by level-one parent species：It goes bud eye, slice to boil peeling potatoes, obtains potato juice, then put agar, wait for agar
Glucose, peptone, potassium dihydrogen phosphate, magnesium sulfate, vitamin B are added after dissolving1, sodium selenite and water, be made 1000 milliliters
Culture medium dispenses test tube high pressure sterilization, when pressure reaches 0.05MPa, lets cool air back to zero, is pressurized to 0.14MPa and maintains 30 points
Clock back to zero, taking-up is put into inclined-plane, when temperature is down to 30 DEG C, mushroom kind of aseptically transferring, through being cultivated under 25 DEG C of constant temperature
It covers with inclined-plane within 15 days or so, obtains level-one kind；
(2) prepared by two level parent species：Mix packing seed bottle thoroughly, tampon sealing sterilizing, it is zero that pressure cools down in 0.05MPa, back to zero
When pressurization rises to 0.14MPa afterwards, continue 3 hours, temperature is down to 30 DEG C or less aseptic condition access level-one parent species, every level-one mother
Kind 5-8 bottles of original seeds of switching, cultivate 40 days or so in 25 DEG C of dark surrounds and obtain two level kind；
(3) prepared by three-level parent species：Cultigen is produced with polyethylene bagging, normal-pressure sterilization, remains 48 small when temperature is up to 100 DEG C again
When, when the back pkt. that takes the dish out of the pot is cooled to 30 DEG C or less, two level kind is added under aseptic condition, three-level kind is obtained by culture；
(4) Se-rich xianggu is cultivated：It adds liquid organic selenium fertilizer again in medium culture material, is produced with polyethylene bagging, normal pressure
Sterilizing, temperature continue 48 hours up to 100 DEG C, and temperature is down to 30 DEG C and makes a living hereinafter, accessing three-level kind under aseptic condition after taking the dish out of the pot
Produce bag.
2. a kind of Se-rich xianggu production technology according to claim 1, it is characterised in that：Potato in the step (1)
200g, glucose 20g, agar 20-25g, peptone 1-3g, phosphatide potassium dihydrogen 0.5-1.5g, magnesium sulfate 0.5-1.5g, vitamin
B10.5-1.5g, appropriate sodium selenite, 1000 milliliters of water.
3. a kind of Se-rich xianggu production technology according to claim 1, it is characterised in that：In the step (2) by weight
Ratio chooses raw material：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, appropriate sodium selenite, material water weight ratio are 1:
1.2。
4. a kind of Se-rich xianggu production technology according to claim 1, it is characterised in that：In the step (3) by weight
Ratio chooses raw material：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, appropriate sodium selenite, material water weight ratio are 1:
1.2。
5. a kind of Se-rich xianggu production technology according to claim 1, it is characterised in that：In the step (4) by weight
Ratio chooses raw material：Sawdust 78%, wheat bran 20%, magnesium sulfate 1%, gypsum 1%, appropriate sodium selenite, per kilogram compost add
1.5-1.6 milliliters of organic selenium fertilizer, material-water ratio example are 1:1.2.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109348990A (en) * | 2018-12-06 | 2019-02-19 | 四川农业大学 | A method of improving 808 polyoses content of mushroom kind |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496484A (en) * | 2008-01-31 | 2009-08-05 | 马俊生 | Method for domesticating and cultivating selenium-rich mushroom strain |
-
2018
- 2018-05-14 CN CN201810454063.2A patent/CN108617403A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101496484A (en) * | 2008-01-31 | 2009-08-05 | 马俊生 | Method for domesticating and cultivating selenium-rich mushroom strain |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109348990A (en) * | 2018-12-06 | 2019-02-19 | 四川农业大学 | A method of improving 808 polyoses content of mushroom kind |
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Application publication date: 20181009 |