CN108601883A - 递送方法和组合物 - Google Patents
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- CN108601883A CN108601883A CN201680068693.5A CN201680068693A CN108601883A CN 108601883 A CN108601883 A CN 108601883A CN 201680068693 A CN201680068693 A CN 201680068693A CN 108601883 A CN108601883 A CN 108601883A
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- A61K9/0009—Galenical forms characterised by the drug release technique; Application systems commanded by energy involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/18—Applying electric currents by contact electrodes
- A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
- A61N1/327—Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A61P31/12—Antivirals
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/20—Antivirals for DNA viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M2037/0007—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin having means for enhancing the permeation of substances through the epidermis, e.g. using suction or depression, electric or magnetic fields, sound waves or chemical agents
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- A—HUMAN NECESSITIES
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- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0092—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin using ultrasonic, sonic or infrasonic vibrations, e.g. phonophoresis
Landscapes
- Health & Medical Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Public Health (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Epidemiology (AREA)
- Gastroenterology & Hepatology (AREA)
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- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Communicable Diseases (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Dermatology (AREA)
- Medical Informatics (AREA)
- Anesthesiology (AREA)
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| US62/234,340 | 2015-09-29 | ||
| PCT/US2016/053960 WO2017058793A1 (en) | 2015-09-29 | 2016-09-27 | Delivery methods and compositions |
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| CN108601883A true CN108601883A (zh) | 2018-09-28 |
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| EP (1) | EP3355954A4 (enExample) |
| JP (1) | JP2018532403A (enExample) |
| CN (1) | CN108601883A (enExample) |
| AU (1) | AU2016332704A1 (enExample) |
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| HK (1) | HK1258900A1 (enExample) |
| WO (1) | WO2017058793A1 (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020015279A1 (zh) * | 2018-07-17 | 2020-01-23 | 杭州观梓健康科技有限公司 | 一种在干细胞中进行基因定向敲入的方法 |
| CN112807273A (zh) * | 2021-02-23 | 2021-05-18 | 浙江大学 | 一种治疗炎症性皮肤病的基因编辑微针及其应用 |
| CN113556970A (zh) * | 2019-04-08 | 2021-10-26 | 维兰尼迪科技有限责任公司 | 用于在微纳米环境中感测、检测和实现的系统、设备和方法 |
| CN114072527A (zh) * | 2019-03-25 | 2022-02-18 | 香港中文大学 | 确定循环核酸的线性和环状形式 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
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| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
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| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
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| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
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| JP2021508533A (ja) | 2017-12-26 | 2021-03-11 | ギャラリー，インコーポレイテッド | 様々な用途のためのエネルギー送達の最適化 |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| WO2020102532A1 (en) * | 2018-11-15 | 2020-05-22 | President And Fellows Of Harvard College | Genetically engineered skin cells for the systemic in vivo treatment of deficient enzymes, factors or proteins |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
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| CN116829691A (zh) * | 2020-08-04 | 2023-09-29 | 盖能适治疗股份有限公司 | 分子到体内细胞的脉冲电场转移 |
| JP2023094496A (ja) * | 2021-12-23 | 2023-07-05 | 委子 陌間 | 抗菌・ウイルス治療薬の製造方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040203124A1 (en) * | 2001-08-03 | 2004-10-14 | King Alan D. | Electrodes coated with treating agent and uses thereof |
| US20130046230A1 (en) * | 2010-03-05 | 2013-02-21 | Cornell University | Ultrasound-assisted convection enhanced delivery of compounds in vivo with a transducer cannula assembly |
| WO2015089465A1 (en) * | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for hbv and viral diseases and disorders |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1432799A2 (en) * | 2001-07-17 | 2004-06-30 | Anne Josephine Milner | Silencing of gene expression by sirna |
| ES2751916T3 (es) * | 2010-02-08 | 2020-04-02 | Sangamo Therapeutics Inc | Semidominios de escisión genomanipulados |
| KR20160130392A (ko) * | 2014-02-18 | 2016-11-11 | 듀크 유니버시티 | 바이러스 복제의 불활성화를 위한 조성물 및 그의 제조 및 사용 방법 |
| US20160060655A1 (en) * | 2014-05-30 | 2016-03-03 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods to treat latent viral infections |
| CA3000155A1 (en) * | 2015-05-29 | 2016-12-08 | Agenovir Corporation | Compositions and methods for cell targeted hpv treatment |
| WO2017147446A1 (en) * | 2016-02-25 | 2017-08-31 | Agenovir Corporation | Viral and oncoviral nuclease treatment |
-
2016
- 2016-09-27 JP JP2018516030A patent/JP2018532403A/ja active Pending
- 2016-09-27 US US15/277,595 patent/US20170087224A1/en not_active Abandoned
- 2016-09-27 AU AU2016332704A patent/AU2016332704A1/en not_active Abandoned
- 2016-09-27 CA CA2999922A patent/CA2999922A1/en not_active Abandoned
- 2016-09-27 WO PCT/US2016/053960 patent/WO2017058793A1/en not_active Ceased
- 2016-09-27 HK HK19101376.1A patent/HK1258900A1/zh unknown
- 2016-09-27 CN CN201680068693.5A patent/CN108601883A/zh active Pending
- 2016-09-27 EP EP16852411.4A patent/EP3355954A4/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040203124A1 (en) * | 2001-08-03 | 2004-10-14 | King Alan D. | Electrodes coated with treating agent and uses thereof |
| US20130046230A1 (en) * | 2010-03-05 | 2013-02-21 | Cornell University | Ultrasound-assisted convection enhanced delivery of compounds in vivo with a transducer cannula assembly |
| WO2015089465A1 (en) * | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for hbv and viral diseases and disorders |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020015279A1 (zh) * | 2018-07-17 | 2020-01-23 | 杭州观梓健康科技有限公司 | 一种在干细胞中进行基因定向敲入的方法 |
| CN114072527A (zh) * | 2019-03-25 | 2022-02-18 | 香港中文大学 | 确定循环核酸的线性和环状形式 |
| CN113556970A (zh) * | 2019-04-08 | 2021-10-26 | 维兰尼迪科技有限责任公司 | 用于在微纳米环境中感测、检测和实现的系统、设备和方法 |
| CN112807273A (zh) * | 2021-02-23 | 2021-05-18 | 浙江大学 | 一种治疗炎症性皮肤病的基因编辑微针及其应用 |
| CN112807273B (zh) * | 2021-02-23 | 2022-06-17 | 浙江大学 | 一种治疗炎症性皮肤病的基因编辑微针及其应用 |
Also Published As
| Publication number | Publication date |
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| CA2999922A1 (en) | 2017-04-06 |
| EP3355954A4 (en) | 2020-01-08 |
| WO2017058793A1 (en) | 2017-04-06 |
| HK1258900A1 (zh) | 2019-11-22 |
| US20170087224A1 (en) | 2017-03-30 |
| JP2018532403A (ja) | 2018-11-08 |
| AU2016332704A1 (en) | 2018-04-19 |
| EP3355954A1 (en) | 2018-08-08 |
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