CN108543003A - Anticancer herbal drug is the temperature of raw material through disperse accumulation pharmaceutical composition - Google Patents
Anticancer herbal drug is the temperature of raw material through disperse accumulation pharmaceutical composition Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/06—Fungi, e.g. yeasts
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/235—Foeniculum (fennel)
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
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- A61K36/77—Sapindaceae (Soapberry family), e.g. lychee or soapberry
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- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8964—Anemarrhena
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
It is the warm through disperse accumulation pharmaceutical composition of raw material that the present invention, which provides anticancer herbal drug, belongs to the field of Chinese medicines, contains the components such as 13 45 parts of cassia twig, granatum, fennel, moutan bark, rhizoma anemarrhenae, root of Chinese trichosanthes, matrimony vine, semen litchi, Rubus corchorifolius, Antrodia camphorata hypha powder.The present invention obtains the dregs of a decoction using extraction and is used for Antrodia camphorata culture, utilize extra effective nutritional ingredient in the dregs of a decoction, cultivate obtained antrodia mycelia, biomass is high, intracellular triterpene yield is high, wherein mycelial biomass reaches 14.3~16g/1000ml, and intracellular triterpene yield reaches 43.65~50.78mg/g.This mycelium is added in pharmaceutical composition, it cooperates with other components, form good synergistic effect, pharmaceutical composition obtained has dispelling cold in temperature, warming and activating meridian, fragrant invigorating the spleen, supporing yang leads to inverse stagnant, promoting qi circulation and relieving pain, pleasant drop, soothing liver-qi stagnation, alleviate long-pending dissipating bind and other effects.
Description
Technical field
The invention belongs to the field of Chinese medicines, and in particular to anticancer herbal drug is the temperature of raw material through disperse accumulation pharmaceutical composition.
Background technology
In some Chinese patent drugs promoting blood circulation and removing blood stasis used at present, shortage is warming channel and expelling cold, except stagnant dredging collateral, the medicine for the treatment of both manifestation and root cause of disease
Object.Some chronic infections include caused extravasated blood phenomenon after some infection, not meet " inflammation must be clearing heat and detoxicating " general rule
Rule usually has the feature of cloudy card, cold card on the contrary, some chronic inflammations are especially the chronic inflammation that some do not recover for a long time.
In addition with since Chinese medicine is made of autonomic drug and animal drugs mostly, how pungent flavour of a drug are, sour, bitter, salty, when sufferer uses not
But it is inconvenient to decoct, and flavour of a drug are unpleasant, takes decoction of medicinal ingredients bitter difficulty pharynx orally, easily generates stimulation damage to stomach and alimentary canal, significantly
Affect the development of traditional Chinese medicine.Therefore, the present invention is directed to the problem of currently available technology, provides a kind of while having temperature
Pharmaceutical composition through disperse accumulation can be used for preparing various preparations, realize industrialization.
Invention content
The purpose of the present invention is to provide the temperature that anticancer herbal drug is raw material through disperse accumulation pharmaceutical composition.
To achieve the above object, the present invention adopts the following technical scheme that：
Anticancer herbal drug is the warm through disperse accumulation pharmaceutical composition of raw material, and the composition contains following raw material：Cassia twig 13-45
Part, 7-30 parts of granatum, 5-10 parts of fennel, 2.5-12 parts of moutan bark, 5-20 parts of rhizoma anemarrhenae, 6-20 parts of root of Chinese trichosanthes, matrimony vine 10-35
Part, 8-19 parts of semen litchi, 5-16 parts of Rubus corchorifolius, 2-5 parts of antrodia mycelia powder.
The anticancer herbal drug is the warm preparation method through disperse accumulation pharmaceutical composition of raw material, is included the following steps：
（1）13-45 parts of Chinese herbal medicine cassia twig, 7-30 parts of granatum, 5-10 parts of fennel, 2.5-12 parts of moutan bark, 5-20 parts of rhizoma anemarrhenae, day
5-16 parts of 6-20 parts of pollen, 10-35 parts of matrimony vine, 8-19 parts of semen litchi, Rubus corchorifolius removing impurity, it is dry, for use；
（2）Moutan bark is soaked into 3.5h, extraction by steam distillation Paeonol collects 90 times that distillate is medicinal material, medicine
Liquid and the dregs of a decoction are separately deposited, and are refrigerated at 1 DEG C of distillate, leaching Paeonol, and Paeonol crude product is obtained after drying, and refrigeration is spare；
（3）Cassia twig and granatum add the water of 6 times of weight to impregnate 2h, then with water boiling 1.5h, filtering and collecting filter liquid, continue plus
Enter 4 times of weight waters and continue boiling 1.5h, collect filtrate, filtrate will mix for use twice, the dregs of a decoction are for use；
（4）Take step（2）、（3）Chinese medicine slag mix, fennel, rhizoma anemarrhenae, root of Chinese trichosanthes, matrimony vine, semen litchi, Rubus corchorifolius crush after with the dregs of a decoction
Mixing is added 3 times of 65% alcohol refluxs of weight and extracts 2 times, alcohol extract and the dregs of a decoction is collected, after alcohol extract recycles ethyl alcohol, with step
（2）Moutan bark liquid and step（3）Cassia twig filtrate mixes, and 1.2 or more medicinal extract of relative density is concentrated at 50-60 DEG C, by true
Medicinal powder is obtained after vacuum freecing-dry；
（5）Step（4）It is ground into powder after dregs of a decoction drying and is configured to Antrodia camphorata culture medium and be：Dregs of a decoction powder 30g/L, glucose
10g, potassium dihydrogen phosphate 5g/L, corn flour 5g/L, VB1 0.1g/L, pH 5.0 being adjusted, 121 DEG C of high steam sterilizes 20 minutes,
It takes out, the Antrodia camphorata fungus block of 1~2cm sizes is seeded in Antrodia cinnamomea growth medium after cooling, in rotating speed after the completion of sterilizing
150/min is cultivated 3 days at 25 DEG C of temperature, collects mycelium, and dries pulverizing obtains antrodia mycelia powder；
（6）By Paeonol crude product according to 1:The ratio of 5g/g is added in 80% ethyl alcohol, is stirred evenly and is made it dissolve fully, then
It is added step（4）Chinese herbal medicine powder and 2-5 parts of antrodia mycelia powder are obtained, 2h is stirred at 35 DEG C, ethyl alcohol is recovered by filtration, it will
It is the warm through disperse accumulation pharmaceutical composition of raw material that mixture freeze-drying, which obtains anticancer herbal drug,.
The advantage of the invention is that：
Cassia twig energy warming meridian in full side, moutan bark can be promoting blood circulation and removing blood stasis, are for monarch drug in a prescription.Granatum is Hua Yu Xiao wei；Rhizoma anemarrhenae is fallen
Burnt kidney is fought enriching yin, matrimony vine blood-enriching tranquillizing, and fennel has the effect of stomach invigorating promoting the circulation of qi, can enhance the solution Yu Xiaoguo of cassia twig, there is collaboration
Synergistic effect is altogether ministerial drug.Root of Chinese trichosanthes clearing heat-fire；Semen litchi is apt to walk Liver Channel blood system, work(benefaction gas, cold dispelling, analgesic；Rubus corchorifolius is lived
Blood, hemostasis, is altogether adjuvant, strengthens the body resistance to consolidate the constitution altogether, repairs train of thought, the promoting flow of qi and blood, up to heightening the effect of a treatment.All medicines share makes Wen Jing altogether
The effect of disperse accumulation.
The present invention extract obtain paeonol in Cortex Moutan, effectively increase drug component it is promoting blood circulation and removing blood stasis the effect of, simultaneously
The effect of playing synergistic effect jointly with Antrodia camphorata hypha powder, effectively increase drug component, while avoiding drug to body
Damage improves immunity.
The present invention uses the remaining dregs of a decoction after each traditional Chinese medicine extraction, is used for the culture of Antrodia camphorata, the Antrodia camphorata cultivated
Mycelium, biomass is high, and intracellular triterpene yield is high, and wherein mycelial biomass reaches 14.3~16g/1000ml, intracellular triterpene
Yield reaches 43.65~50.78mg/g.This mycelium is added in pharmaceutical composition, is cooperated with other components, is formed
There is dispelling cold in temperature, warming and activating meridian, fragrant invigorating the spleen, supporing yang logical stagnant, promoting the circulation of qi to stop for good synergistic effect, pharmaceutical composition obtained
Bitterly, pleasant to drop inverse, soothing liver-qi stagnation, alleviate long-pending dissipating bind and other effects.The dregs of a decoction are recycled simultaneously, avoid waste and the pollution of environment.
Specific implementation mode
Embodiment 1
Anticancer herbal drug is the warm through disperse accumulation pharmaceutical composition of raw material, and the composition contains following raw material：30 parts of cassia twig,
20 parts of granatum, 7 parts of fennel, 8 parts of moutan bark, 10 parts of rhizoma anemarrhenae, 12 parts of root of Chinese trichosanthes, 25 parts of matrimony vine, 15 parts of semen litchi, Rubus corchorifolius 10
Part, 3 parts of antrodia mycelia powder.
The anticancer herbal drug is the warm preparation method through disperse accumulation pharmaceutical composition of raw material, is included the following steps：
（1）30 parts of Chinese herbal medicine cassia twig, 20 parts of granatum, 7 parts of fennel, 8 parts of moutan bark, 10 parts of rhizoma anemarrhenae, 12 parts of root of Chinese trichosanthes, matrimony vine 25
10 parts of part, 15 parts of semen litchi, Rubus corchorifolius removing impurity, it is dry, for use；
（2）Moutan bark is soaked into 3.5h, extraction by steam distillation Paeonol collects 90 times that distillate is medicinal material, medicine
Liquid and the dregs of a decoction are separately deposited, and are refrigerated at 1 DEG C of distillate, leaching Paeonol, and Paeonol crude product is obtained after drying, and refrigeration is spare；
（3）Cassia twig and granatum add the water of 6 times of weight to impregnate 2h, then with water boiling 1.5h, filtering and collecting filter liquid, continue plus
Enter 4 times of weight waters and continue boiling 1.5h, collect filtrate, filtrate will mix for use twice, the dregs of a decoction are for use；
（4）Take step（2）、（3）Chinese medicine slag mix, fennel, rhizoma anemarrhenae, root of Chinese trichosanthes, matrimony vine, semen litchi, Rubus corchorifolius crush after with the dregs of a decoction
Mixing is added 3 times of 65% alcohol refluxs of weight and extracts 2 times, alcohol extract and the dregs of a decoction is collected, after alcohol extract recycles ethyl alcohol, with step
（2）Moutan bark liquid and step（3）Cassia twig filtrate mixes, and 1.2 medicinal extract of relative density is concentrated at 55 DEG C, by vacuum refrigeration
Medicinal powder is obtained after drying；
（5）Step（4）It is ground into powder after dregs of a decoction drying and is configured to Antrodia camphorata culture medium and be：Dregs of a decoction powder 30g/L, glucose
10g, potassium dihydrogen phosphate 5g/L, corn flour 5g/L, VB1 0.1g/L, pH 5.0 being adjusted, 121 DEG C of high steam sterilizes 20 minutes,
It takes out, the Antrodia camphorata fungus block of 2cm sizes is seeded in Antrodia cinnamomea growth medium after cooling, in rotating speed 150/ after the completion of sterilizing
Min is cultivated 3 days at 25 DEG C of temperature, collects mycelium, and dries pulverizing obtains antrodia mycelia powder；Wherein mycelial biomass
Reach 16g/1000ml, intracellular triterpene yield reaches 50.78mg/g.
（6）By Paeonol crude product according to 1:The ratio of 5g/g is added in 80% ethyl alcohol, is stirred evenly and is made it dissolve fully,
It is subsequently added into step（4）Chinese herbal medicine powder and 3 parts of antrodia mycelia powder are obtained, 2h is stirred at 35 DEG C, ethyl alcohol is recovered by filtration,
It is the warm through disperse accumulation pharmaceutical composition of raw material that mixture, which is freeze-dried, and obtains anticancer herbal drug.
Embodiment 2
Anticancer herbal drug is the warm through disperse accumulation pharmaceutical composition of raw material, and the composition contains following raw material：13 parts of cassia twig,
7 parts of granatum, 5 parts of fennel, 2.5 parts of moutan bark, 5 parts of rhizoma anemarrhenae, 6 parts of root of Chinese trichosanthes, 10 parts of matrimony vine, 8 parts of semen litchi, 5 parts of Rubus corchorifolius, ox
2 parts of Antrodia camphorata mycelium powder.
The anticancer herbal drug is the warm preparation method through disperse accumulation pharmaceutical composition of raw material, is included the following steps：
（1）13 parts of Chinese herbal medicine cassia twig, 7 parts of granatum, 5 parts of fennel, 2.5 parts of moutan bark, 5 parts of rhizoma anemarrhenae, 6 parts of root of Chinese trichosanthes, matrimony vine 10
5 parts of part, 8 parts of semen litchi, Rubus corchorifolius removing impurity, it is dry, for use；
（2）Moutan bark is soaked into 3.5h, extraction by steam distillation Paeonol collects 90 times that distillate is medicinal material, medicine
Liquid and the dregs of a decoction are separately deposited, and are refrigerated at 1 DEG C of distillate, leaching Paeonol, and Paeonol crude product is obtained after drying, and refrigeration is spare；
（3）Cassia twig and granatum add the water of 6 times of weight to impregnate 2h, then with water boiling 1.5h, filtering and collecting filter liquid, continue plus
It is good to enter 4 times of weight waters continuation boilings 1.5, collects filtrate, filtrate will mix for use twice, the dregs of a decoction are for use；
（4）Take step（2）、（3）Chinese medicine slag mix, fennel, rhizoma anemarrhenae, root of Chinese trichosanthes, matrimony vine, semen litchi, Rubus corchorifolius crush after with the dregs of a decoction
Mixing is added 3 times of 65% alcohol refluxs of weight and extracts 2 times, alcohol extract and the dregs of a decoction is collected, after alcohol extract recycles ethyl alcohol, with step
（2）Moutan bark liquid and step（3）Cassia twig filtrate mixes, and 1.3 medicinal extract of relative density is concentrated at 50 DEG C, by vacuum refrigeration
Medicinal powder is obtained after drying；
（5）Step（4）It is ground into powder after dregs of a decoction drying and is configured to Antrodia camphorata culture medium and be：Dregs of a decoction powder 30g/L, glucose
10g, potassium dihydrogen phosphate 5g/L, corn flour 5g/L, VB1 0.1g/L, pH 5.0 being adjusted, 121 DEG C of high steam sterilizes 20 minutes,
It takes out, the Antrodia camphorata fungus block of 1cm sizes is seeded in Antrodia cinnamomea growth medium after cooling, in rotating speed 150/ after the completion of sterilizing
Min is cultivated 3 days at 25 DEG C of temperature, collects mycelium, and dries pulverizing obtains antrodia mycelia powder；Wherein mycelial biomass
Reach 14.3g/1000ml, intracellular triterpene yield reaches 43.65mg/g.
（6）By Paeonol crude product according to 1:The ratio of 5g/g is added in 80% ethyl alcohol, is stirred evenly and is made it dissolve fully,
It is subsequently added into step（4）Chinese herbal medicine powder and 2 parts of antrodia mycelia powder are obtained, 2h is stirred at 35 DEG C, ethyl alcohol is recovered by filtration,
It is the warm through disperse accumulation pharmaceutical composition of raw material that mixture, which is freeze-dried, and obtains anticancer herbal drug.
Embodiment 3
Anticancer herbal drug is the warm through disperse accumulation pharmaceutical composition of raw material, and the composition contains following raw material：45 parts of cassia twig,
30 parts of granatum, 10 parts of fennel, 12 parts of moutan bark, 20 parts of rhizoma anemarrhenae, 20 parts of root of Chinese trichosanthes, 35 parts of matrimony vine, 19 parts of semen litchi, Rubus corchorifolius 16
Part, 5 parts of antrodia mycelia powder.
The anticancer herbal drug is the warm preparation method through disperse accumulation pharmaceutical composition of raw material, is included the following steps：
（1）45 parts of Chinese herbal medicine cassia twig, 30 parts of granatum, 10 parts of fennel, 12 parts of moutan bark, 20 parts of rhizoma anemarrhenae, 20 parts of root of Chinese trichosanthes, matrimony vine
35 parts, 19 parts of semen litchi, 16 parts of Rubus corchorifolius removing impurity, it is dry, for use；
（2）Moutan bark is soaked into 3.5h, extraction by steam distillation Paeonol collects 90 times that distillate is medicinal material, medicine
Liquid and the dregs of a decoction are separately deposited, and are refrigerated at 1 DEG C of distillate, leaching Paeonol, and Paeonol crude product is obtained after drying, and refrigeration is spare；
（3）Cassia twig and granatum add the water of 6 times of weight to impregnate 2h, then with water boiling 1.5h, filtering and collecting filter liquid, continue plus
It is good to enter 4 times of weight waters continuation boilings 1.5, collects filtrate, filtrate will mix for use twice, the dregs of a decoction are for use；
（4）Take step（2）、（3）Chinese medicine slag mix, fennel, rhizoma anemarrhenae, root of Chinese trichosanthes, matrimony vine, semen litchi, Rubus corchorifolius crush after with the dregs of a decoction
Mixing is added 3 times of 65% alcohol refluxs of weight and extracts 2 times, alcohol extract and the dregs of a decoction is collected, after alcohol extract recycles ethyl alcohol, with step
（2）Moutan bark liquid and step（3）Cassia twig filtrate mixes, and 1.5 medicinal extract of relative density is concentrated at 60 DEG C, by vacuum refrigeration
Medicinal powder is obtained after drying；
（5）Step（4）It is ground into powder after dregs of a decoction drying and is configured to Antrodia camphorata culture medium and be：Dregs of a decoction powder 30g/L, glucose
10g, potassium dihydrogen phosphate 5g/L, corn flour 5g/L, VB1 0.1g/L, pH 5.0 being adjusted, 121 DEG C of high steam sterilizes 20 minutes,
It takes out, the Antrodia camphorata fungus block of 1cm sizes is seeded in Antrodia cinnamomea growth medium after cooling, in rotating speed 150/ after the completion of sterilizing
Min is cultivated 3 days at 25 DEG C of temperature, collects mycelium, and dries pulverizing obtains antrodia mycelia powder；Wherein mycelial biomass
Reach 15.3g/1000ml, intracellular triterpene yield reaches 45.61mg/g.
（6）By Paeonol crude product according to 1:The ratio of 5g/g is added in 80% ethyl alcohol, is stirred evenly and is made it dissolve fully,
It is subsequently added into step（4）Chinese herbal medicine powder and 5 parts of antrodia mycelia powder are obtained, 2h is stirred at 35 DEG C, ethyl alcohol is recovered by filtration,
It is the warm through disperse accumulation pharmaceutical composition of raw material that mixture, which is freeze-dried, and obtains anticancer herbal drug.
Application Example
The pharmaceutical composition of embodiment 1 is prepared into capsule according to a conventional method.
1, ordinary circumstance and test method
To confirm the clinical efficacy of drug of the present invention, in April, 2010 in by the end of March, -2011, treatment group selection obstruction of fallopian tube
100, wherein both sides obstruction of fallopian tube 14, side obstruction 30, hydrosalpinx 26, logical and unsmooth 30；The course of disease is
It is -3.4 years 1.1 years, 1.7 years average；Control group obstruction of fallopian tube 100, wherein both sides obstruction of fallopian tube 14, side obstruction 30
Example, hydrosalpinx 26, logical and unsmooth 30.The course of disease is -3.5 years 1.0 years, average year 1.8.Two groups are statistically analyzed tool
There is a comparativity, difference there are no significant meaning (P ＞ 0.05).
1 medicament capsule of embodiment is all taken by treatment group, 2 tablets each time, 3 times a day.Control group takes orally GUIZHI FULING JIAONANG
(Kangyuan Pharmaceutical Co., Ltd., Jiangsu Prov produces, every 0.31g), 2 tablets each time, 3 times a day.Two groups of administration times are 1
The course for the treatment of (60d).
2, clinical observation standard
Case diagnosis is made a definite diagnosis by fallopian tubal hysterography (contrast agent is lipiodol or general shadow Pu amine).
Criterion of therapeutical effect：3-7 days of next menstrual cycle clean period are checked after 1 course of therapy.Fallopian tubal is complete
All-pass is smooth, ponding disappears or become pregnant for recovery from illness；Fallopian tubal obstruction completely switchs to partial outlet obstruction, ponding eliminates 2/3 or more to have
Effect；It is invalid that fallopian tubal, which is not improved, after treatment.
3, therapeutic effect is analyzed
Treatment group's healing 60, effective 35, invalid 5, total effective rate 95.0%；Control group healing 24, effective 40,
Invalid 36, total effective rate 64%.Two groups of comparing differences are statistically significant (P ＜ 0.05).
For influence of the observation drug therapy to whole blood, two groups of (each to check 30) patients before treatment and after treatment in
Menstrual period venous blood samples measure whole blood viscosity (height is cut) and two parameters of fibrinogen, and pretherapy and post-treatment follicular phase measures bilateral
Arteria ovarica pulsating index and drag index.As a result, it has been found that treatment group is significant in efficacy to be higher than control group, and it is without side-effects.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification should all belong to the covering scope of the present invention.
Claims (2)
1. anticancer herbal drug is the temperature of raw material through disperse accumulation pharmaceutical composition, it is characterised in that：The composition contains following parts by weight
Raw material：13-45 parts of cassia twig, 7-30 parts of granatum, 5-10 parts of fennel, 2.5-12 parts of moutan bark, 5-20 parts of rhizoma anemarrhenae, root of Chinese trichosanthes 6-20
Part, 10-35 parts of matrimony vine, 8-19 parts of semen litchi, 5-16 parts of Rubus corchorifolius, 2-5 parts of Antrodia camphorata hypha powder.
2. anticancer herbal drug as described in claim 1 is the warm preparation method through disperse accumulation pharmaceutical composition of raw material, feature
It is：Include the following steps：
（1）13-45 parts of Chinese herbal medicine cassia twig, 7-30 parts of granatum, 5-10 parts of fennel, 2.5-12 parts of moutan bark, 5-20 parts of rhizoma anemarrhenae, day
5-16 parts of 6-20 parts of pollen, 10-35 parts of matrimony vine, 8-19 parts of semen litchi, Rubus corchorifolius removing impurity, it is dry, for use；
（2）Moutan bark is soaked into 3.5h, extraction by steam distillation Paeonol collects 90 times that distillate is medicinal material, medicine
Liquid and the dregs of a decoction are separately deposited, and are refrigerated at 1 DEG C of distillate, leaching Paeonol, and Paeonol crude product is obtained after drying, and refrigeration is spare；
（3）Cassia twig and granatum add the water of 6 times of weight to impregnate 2h, then with water boiling 1.5h, filtering and collecting filter liquid, continue plus
It is good to enter 4 times of weight waters continuation boilings 1.5, collects filtrate, filtrate will mix for use twice, the dregs of a decoction are for use；
（4）Take step（2）、（3）Chinese medicine slag mix, fennel, rhizoma anemarrhenae, root of Chinese trichosanthes, matrimony vine, semen litchi, Rubus corchorifolius crush after with the dregs of a decoction
Mixing is added 3 times of 65% alcohol refluxs of weight and extracts 2 times, alcohol extract and the dregs of a decoction is collected, after alcohol extract recycles ethyl alcohol, with step
（2）Moutan bark liquid and step（3）Cassia twig filtrate mixes, and 1.2 or more medicinal extract of relative density is concentrated at 50-60 DEG C, by true
Medicinal powder is obtained after vacuum freecing-dry；
（5）Step（4）It is ground into powder after dregs of a decoction drying and is configured to Antrodia camphorata culture medium and be：Dregs of a decoction powder 30g/L, glucose
10g, potassium dihydrogen phosphate 5g/L, corn flour 5g/L, VB1 0.1g/L, pH 5.0 being adjusted, 121 DEG C of high steam sterilizes 20 minutes,
It takes out, the Antrodia camphorata fungus block of 1~2cm sizes is seeded in Antrodia cinnamomea growth medium after cooling, in rotating speed after the completion of sterilizing
150/min is cultivated 3 days at 25 DEG C of temperature, collects mycelium, and dries pulverizing obtains Antrodia camphorata hypha powder；
（6）By Paeonol crude product according to 1:The ratio of 5g/g is added in 80% ethyl alcohol, is stirred evenly and is made it dissolve fully, then
It is added step（4）Chinese herbal medicine powder and 2-5 parts of Antrodia camphorata hypha powders are obtained, 2h is stirred at 35 DEG C, ethyl alcohol is recovered by filtration, will mix
It is the warm through disperse accumulation pharmaceutical composition of raw material to close object freeze-drying and obtain anticancer herbal drug.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613430A (en) * | 2013-11-25 | 2014-03-05 | 浙江科技学院 | Antrodia cinnamomea growth medium and preparation method and application thereof |
| CN103784663A (en) * | 2014-01-15 | 2014-05-14 | 闫玉枝 | Traditional Chinese medicine for treating tubal obstruction |
| CN104212726A (en) * | 2014-09-17 | 2014-12-17 | 中山安荞生物科技有限公司 | Liquid fermentation culture medium for antrodia camphorata |
-
2018
- 2018-06-04 CN CN201810565077.1A patent/CN108543003A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613430A (en) * | 2013-11-25 | 2014-03-05 | 浙江科技学院 | Antrodia cinnamomea growth medium and preparation method and application thereof |
| CN103784663A (en) * | 2014-01-15 | 2014-05-14 | 闫玉枝 | Traditional Chinese medicine for treating tubal obstruction |
| CN104212726A (en) * | 2014-09-17 | 2014-12-17 | 中山安荞生物科技有限公司 | Liquid fermentation culture medium for antrodia camphorata |
Non-Patent Citations (1)
| Title |
|---|
| 罗洁等: "桂枝茯苓胶囊治疗输卵管阻塞性不孕120例", 《现代中西医结合杂志》 * |
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Application publication date: 20180918 |