CN108531503B - 优化拟南芥转基因效率的方法 - Google Patents
优化拟南芥转基因效率的方法 Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
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- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
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CN109566402B (zh) * | 2019-01-14 | 2021-09-14 | 安徽农业大学 | 一种快速同步获得乌菜雄性不育与保持系转基因植株方法 |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101979602A (zh) * | 2010-10-08 | 2011-02-23 | 中国农业科学院油料作物研究所 | 一种超声辅助农杆菌介导的植物in planta遗传转化方法 |
CN103451190A (zh) * | 2013-09-05 | 2013-12-18 | 南开大学 | 一个花椰菜器官发育调控基因编码序列及其应用 |
CN104878042A (zh) * | 2015-06-23 | 2015-09-02 | 东北农业大学 | 一种提高植物抗逆性的方法及其应用 |
CN105524156A (zh) * | 2016-01-26 | 2016-04-27 | 甘肃农业大学 | 抗逆相关基因ZmHDZIV14在调控植物抗逆性中的应用方法 |
CN107446928A (zh) * | 2017-09-25 | 2017-12-08 | 南开大学 | 一个花椰菜器官发育调控miRNA序列及其应用 |
WO2017220539A1 (en) * | 2016-06-20 | 2017-12-28 | Algentech | Protein production in plant cells |
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Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101979602A (zh) * | 2010-10-08 | 2011-02-23 | 中国农业科学院油料作物研究所 | 一种超声辅助农杆菌介导的植物in planta遗传转化方法 |
CN103451190A (zh) * | 2013-09-05 | 2013-12-18 | 南开大学 | 一个花椰菜器官发育调控基因编码序列及其应用 |
CN104878042A (zh) * | 2015-06-23 | 2015-09-02 | 东北农业大学 | 一种提高植物抗逆性的方法及其应用 |
CN105524156A (zh) * | 2016-01-26 | 2016-04-27 | 甘肃农业大学 | 抗逆相关基因ZmHDZIV14在调控植物抗逆性中的应用方法 |
WO2017220539A1 (en) * | 2016-06-20 | 2017-12-28 | Algentech | Protein production in plant cells |
CN107446928A (zh) * | 2017-09-25 | 2017-12-08 | 南开大学 | 一个花椰菜器官发育调控miRNA序列及其应用 |
Non-Patent Citations (1)
Title |
---|
用氯化钠溶液克服油菜上的自交不亲和性;余天;《种子世界》;19891231(第10期);第24-25页 * |
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Application publication date: 20180914 Assignee: Bobai Dayu agriculture and animal husbandry Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980045130 Denomination of invention: Method for optimizing the efficiency of transgenic Arabidopsis thaliana Granted publication date: 20210525 License type: Common License Record date: 20231101 |
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Application publication date: 20180914 Assignee: Bobai Dayu agriculture and animal husbandry Co.,Ltd. Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS Contract record no.: X2023980046539 Denomination of invention: Method for optimizing the efficiency of transgenic Arabidopsis thaliana Granted publication date: 20210525 License type: Common License Record date: 20231108 |
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