CN108522371A - A kind of hatching method of black pearl Medaka fish resting egg - Google Patents
A kind of hatching method of black pearl Medaka fish resting egg Download PDFInfo
- Publication number
- CN108522371A CN108522371A CN201810237523.6A CN201810237523A CN108522371A CN 108522371 A CN108522371 A CN 108522371A CN 201810237523 A CN201810237523 A CN 201810237523A CN 108522371 A CN108522371 A CN 108522371A
- Authority
- CN
- China
- Prior art keywords
- hatching
- ovum
- egg
- resting egg
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000012447 hatching Effects 0.000 title claims abstract description 81
- 230000000284 resting effect Effects 0.000 title claims abstract description 46
- 241000276569 Oryzias latipes Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 39
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 73
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 73
- 210000004681 ovum Anatomy 0.000 claims abstract description 70
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 58
- 239000007921 spray Substances 0.000 claims abstract description 21
- 239000003814 drug Substances 0.000 claims abstract description 8
- 229940079593 drug Drugs 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims abstract description 3
- 239000002689 soil Substances 0.000 claims description 18
- 239000003415 peat Substances 0.000 claims description 16
- 229920000742 Cotton Polymers 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 12
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical class O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 11
- 238000005507 spraying Methods 0.000 claims description 10
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 6
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 6
- 238000003860 storage Methods 0.000 claims description 6
- 239000003755 preservative agent Substances 0.000 claims description 5
- 230000002335 preservative effect Effects 0.000 claims description 5
- 239000003595 mist Substances 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 abstract description 34
- 230000018109 developmental process Effects 0.000 abstract description 9
- 238000011161 development Methods 0.000 abstract description 5
- 238000011534 incubation Methods 0.000 abstract description 5
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 230000008859 change Effects 0.000 description 6
- 241000251468 Actinopterygii Species 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 5
- 230000006266 hibernation Effects 0.000 description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 3
- 230000005059 dormancy Effects 0.000 description 3
- 235000014103 egg white Nutrition 0.000 description 3
- 210000000969 egg white Anatomy 0.000 description 3
- 230000017448 oviposition Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 235000014653 Carica parviflora Nutrition 0.000 description 1
- 244000132059 Carica parviflora Species 0.000 description 1
- 235000001543 Corylus americana Nutrition 0.000 description 1
- 240000007582 Corylus avellana Species 0.000 description 1
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- NIPNSKYNPDTRPC-UHFFFAOYSA-N N-[2-oxo-2-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 NIPNSKYNPDTRPC-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000008144 egg development Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010539 reproductive behavior Effects 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention relates to hatching methods, disclose a kind of hatching method of black pearl Medaka fish resting egg, the method includes the steps of：1）The preservation of resting egg；2）The pretreatment of resting egg；3）Lower water hatching；4）Do not hatch ovum two to incubate.The present invention preserves fertilized eggs by using no earthwork formula, it is capable of the development condition of accurate judgement fish-egg, keep brooding time node more accurate, accelerate developmental process by heating, drug spray, hatching rate improves 30% compared with traditional incubation method, and hatching number only needs 2 times, brooding time greatly shortens, without manually choosing ovum, it is greatly saved manpower, is a kind of hatching method of the black pearl Medaka fish resting egg suitable for commercial applications.
Description
Technical field
The present invention relates to hatching method more particularly to a kind of hatching methods of black pearl Medaka fish resting egg.
Background technology
Black pearl Medaka fish belongs to the annual oviparity Medaka fish in South America, there is extremely charming body colour, it may be said that is that fresh water is seen
Only a few can give birth to one of the fingerling that coral reef fish compares favourably with seawater in reward fish, in addition their small and exquisite build, active good
Dynamic personality, it is born perfect match really to be placed in aquatic weed cylinder.In addition, most of oviparity Medaka fishes are easy water quality wide adaptability
Raising, these features make them at home and abroad become extremely welcome one of tropical ornamental fish kind.Especially oviparity Medaka fish by
Smart ovum has a characteristic, can both sell this with the suspend mode regular hour, therefore in the market and can also sell the fish-egg.But
It is that oviparity Medaka fish is extremely special on reproductive behavior：Oviposition is multiple, and does not know in time, and amount is few, and (primary oviposition is at most several
Ten pieces, it is few then one or two piece), fish-egg asked when preserving it is short, the natural Medaka fish-egg that practical market is sold after one month storage rate be only 12-
52%.These reasons cause nature oviparity Medaka fish crop few, some species have been rare in the world endangered species.But
It is the presence of kind of fish egg laying amount is few, rate of fertilization is low, egg hatching rate is low, fry rearing survival rate is low etc. in the market industrialization of Medaka fish
Problems constrain the developing and industry development of ornamental class oviparity Medaka fish market.
Application No. is the Chinese patents of 201610259750.X to disclose a kind of medaka efficiently breeding and hatching method, carries
It has supplied a kind of cultivation controllability is strong, breeding efficiency is high, it can be achieved that medaka is efficiently bred and the method for hatching, but this method needle
To be medaka, different section is belonged in it and the classification of black pearl Medaka fish these oviparity Medaka fishes, is belonged to, after the medaka maturation of ovum,
Female fish by its output, but and without departing from parent, but hung over outside parent cloaca by haircuts, also without rest period, hatching compared with
To be simple, and the hatching of annual Medaka fish, fertilized eggs have to pass through 2-3 months from water suspend mode, even with batch of fertilization
Ovum, brooding time is also not quite identical, therefore is not suitable for very much being commercialized.
Invention content
In order to solve the above technical problem, the present invention provides a kind of hatching methods of black pearl Medaka fish resting egg, pass through
Fertilized eggs are preserved using no earthwork formula, and developmental process is accelerated by heating, drug spray, hatching rate is greatly improved, reduces
Cost is suitable for business application.
The specific technical solution of the present invention is：The method includes the steps of：
1) preservation of resting egg：Resting egg is preserved using no earthwork formula, fish-egg under the conditions of 23-27 DEG C after suspend mode 50 days,
Check the developmental state of ovum；
2) pretreatment of resting egg：When there is resting egg golden eyelet occur, storage temperature is increased 1-2 DEG C, when all
Resting egg all occurs the 14-16 days after golden eyelet, by resting egg taking-up be placed in metallic sieve, with Spawning water to ovum into
Row spraying；
3) water is hatched under：Metallic sieve is placed in hatching cylinder by spraying after terminating 18-22min, hatches and hatching is added in cylinder
With water 13-17cm, metallic sieve upper end 2-4cm in underwater, through 5.5-6.5h, part ovum can hatch, by grit hole to side
It tilts, the prelarva that do not swim out of is made to dissociate sieve；
4) do not hatch ovum two to incubate：Egg white water will not hatched to rinse, drain, be placed on and carry on ovum material, preserved at 27-28 DEG C
10-12 days, then carry out drug spray, the hatching of lower water.
Ovum is often stored in peat soil by traditional incubation mode, for the first time hatch after, also by containing the ovum that do not incubate together with
Peat soil takes out together, drains, then is blotted with newspaper, not only cumbersome, hatching rate is low, but also the prelarva hatched lies prone bottom
Ratio is high；Since ovum is mixed in soil, the development condition of inconvenience observation ovum cannot find accurately and most preferably just incubate the time, and have after just incubating
A small amount of demoulding prelarva is dead because failing free peat soil layer and influences two ovum incubated, and this traditional incubation mode usually wants three to incubate
Or four incubate and can just hatching rate be made to reach 60%；The present invention preserves resting egg using no earthwork formula, is capable of the hair of accurate judgement fish-egg
Situation is educated, keeps brooding time node more accurate.
5 stages of black pearl Medaka fish suspend mode egg development point, this 5 stages can differentiate from color change, be respectively：Thoroughly
It is bright-yellowish it is-deep yellow-there is black eyespot-appearance gold eyelet, the present invention is directed in black pearl Medaka fish growth course to temperature
Demand is different, by heating up when there is golden eyelet, accelerates the developmental process of fish-egg.
The present invention is sprayed by spraying and drug, can stimulate fish-egg demoulding, is reduced hatching number, hatching rate can be made to carry
Rise 30%.
Preferably, in the step 1) without earthwork formula be by resting egg be sealed in by biochemical cotton and preservative film package it is close
In envelope.
Preferably, checking that the developmental state of ovum is that inspection was primary per 2-3 days in the step 1).
Preferably, the preparation method of Spawning water is in the step 2)：The leaching of 9-11wt% peat soils is added in water
Go out liquid and 20-30ppm glutaraldehydes, 26.5-27.5 DEG C of water temperature, with biochemical cotton filter circulation filter 23 day.
Black pearl Medaka fish needs the water quality of weak acid and hazel water colour when hatching, peat soil leachate is added and filters,
It not only can be with regulating water quality, moreover it is possible to prevent from being directly added into peat soil and introducing other impurities, germ.
Preferably, spray method is in the step 2)：Metallic sieve is placed in the hatching cylinder water surface or more, spraying continues
4.5-5.5min sprays 4.5-5.5min again every 18-22min.
Preferably, the specification of hatching cylinder is length, width and height (45-55) cm × (25-35) cm × (20- in the step 3)
30)cm。
Preferably, it is to be soaked in the peat soil leachate of the methylene blue containing 4-6ppm to carry ovum material in the step 4)
The black dense form biochemistry cotton steep, extracted.
Methylene blue can prevent immature fish-egg infection water mold.
Preferably, the store method for not hatching ovum in the step 4) is to fall ovum in carrying on ovum material, ovum material will be carried
Material volume rises, and is sealed in freshness protection package and labelled, indicates fingerling, just incubates date, ovum grain number.
Preferably, drug spraying method is to be sprayed with 80-120ppm glutaraldehydes in the step 4), continue 2-4min.
Preferably, lower water hatching method is that after spray terminates 10min, metallic sieve is placed in hatching in the step 4)
In cylinder, hatches and Spawning water 13-17cm is added in cylinder, metallic sieve upper end is not in the underwater 2-4cm of Spawning water, after 22-26h
Hatching terminates, and fails the ovum of hatching or the prelarva at bottom of lying prone after hatching can eliminate.
The glutaraldehyde of higher concentration can effective stimulus fish-egg demoulding, reduce hatching number.
It is compared with the prior art, the beneficial effects of the invention are as follows：The present invention preserves fertilized eggs, energy by using no earthwork formula
The development condition of enough accurate judgement fish-eggs, keeps brooding time node more accurate, accelerates developmental process by heating, drug spray,
Hatching rate improves 30% compared with traditional incubation method, and hatching number only needs 2 times, and brooding time greatly shortens, without artificial
Ovum is chosen, manpower is greatly saved, is a kind of hatching method of the black pearl Medaka fish resting egg suitable for commercial applications.
Specific implementation mode
With reference to embodiment, the invention will be further described.
Embodiment 1
It is prepared by Spawning water：10wt% peat soils leachate and 20ppm glutaraldehydes, 27 DEG C of water temperature, with life are added in water
Change cotton filter circulation filter 23 day.
Resting egg is sealed in the hermetic bag wrapped up by biochemical cotton and preservative film, fish-egg suspend mode 50 days under the conditions of 25 DEG C
Afterwards, the every 3 days developmental states for checking ovum；When there is resting egg golden eyelet occur, storage temperature is increased 2 DEG C, is stopped when all
All there is the 15th day after golden eyelet in dormancy ovum, and resting egg taking-up is placed in metallic sieve, is sprayed to ovum with Spawning water
Mist sprays and continues 5min, sprays again 5min every 20min；It is length, width and height that metallic sieve, which is placed in specification, after spraying end 20min
In the hatching cylinder of 50cm × 30cm × 25cm, hatches and Spawning water 15cm, metallic sieve upper end 3cm in underwater is added in cylinder,
Through 6h, part ovum can hatch, and grit hole is tilted to side, the prelarva that do not swim out of is made to dissociate sieve；The punching of egg white water will not hatched
It washes, drain, be placed in the peat soil leachate of the methylene blue containing 5ppm and impregnate, on the black dense form biochemistry cotton extracted, will carry
Ovum material is rolled, and is sealed in freshness protection package and labelled, is indicated fingerling, is just incubated date, ovum grain number；It is preserved 11 days at 27 DEG C,
Then it carries out being sprayed with 100ppm glutaraldehydes, continues 3min, after spray terminates 10min, metallic sieve is placed in hatching cylinder, is incubated
Change and Spawning water 15cm is added in cylinder, metallic sieve upper end is hatched after for 24 hours and terminated, fail to incubate not in the underwater 3cm of Spawning water
The prelarva at the ovum of change or bottom of lying prone after hatching can eliminate.
Embodiment 2
It is prepared by Spawning water：9wt% peat soils leachate and 30ppm glutaraldehydes, 26.5 DEG C of water temperature, with life are added in water
Change cotton filter circulation filter 23 day.
Resting egg is sealed in the hermetic bag wrapped up by biochemical cotton and preservative film, fish-egg suspend mode 50 days under the conditions of 27 DEG C
Afterwards, the every 2 days developmental states for checking ovum；When there is resting egg golden eyelet occur, storage temperature is increased 1 DEG C, is stopped when all
All there is the 14th day after golden eyelet in dormancy ovum, and resting egg taking-up is placed in metallic sieve, is sprayed to ovum with Spawning water
Mist sprays and continues 4.5min, sprays again 4.5min every 22min；It is length and width that metallic sieve, which is placed in specification, after spraying end 22min
In the hatching cylinder of high 55cm × 35cm × 20cm, hatch and Spawning water 13cm is added in cylinder, metallic sieve upper end is in underwater
2cm, through 6.5h, part ovum can hatch, and grit hole is tilted to side, the prelarva that do not swim out of is made to dissociate sieve；Ovum will not hatched
Clear water is rinsed, is drained, and is placed in the peat soil leachate of the methylene blue containing 4ppm the black dense form biochemistry cotton impregnated, extracted
On, ovum material will be carried and rolled, sealed in freshness protection package and labelled, fingerling is indicated, just incubate date, ovum grain number；It is protected at 27 DEG C
It deposits 12 days, then carries out being sprayed with 120ppm glutaraldehydes, continue 2min and metallic sieve is placed in hatching after spray terminates 10min
In cylinder, hatch and Spawning water 13cm is added in cylinder, knot is hatched in metallic sieve upper end not in the underwater 2cm of Spawning water after 26h
Beam, fails the ovum of hatching or the prelarva at bottom of lying prone after hatching can eliminate.
Embodiment 3
It is prepared by Spawning water：11wt% peat soils leachate and 25ppm glutaraldehydes are added in water, 27.5 DEG C of water temperature is used
Biochemical cotton filter circulation filter 23 day.
Resting egg is sealed in the hermetic bag wrapped up by biochemical cotton and preservative film, fish-egg suspend mode 50 days under the conditions of 23 DEG C
Afterwards, the every 3 days developmental states for checking ovum；When there is resting egg golden eyelet occur, storage temperature is increased 2 DEG C, is stopped when all
All there is the 16th day after golden eyelet in dormancy ovum, and resting egg taking-up is placed in metallic sieve, is sprayed to ovum with Spawning water
Mist sprays and continues 5.5min, sprays again 5.5min every 18min；It is length and width that metallic sieve, which is placed in specification, after spraying end 18min
In the hatching cylinder of high 45cm × 25cm × 30cm, hatch and Spawning water 17cm is added in cylinder, metallic sieve upper end is in underwater
4cm, through 5.5h, part ovum can hatch, and grit hole is tilted to side, the prelarva that do not swim out of is made to dissociate sieve；Ovum will not hatched
Clear water is rinsed, is drained, and is placed in the peat soil leachate of the methylene blue containing 6ppm the black dense form biochemistry cotton impregnated, extracted
On, ovum material will be carried and rolled, sealed in freshness protection package and labelled, fingerling is indicated, just incubate date, ovum grain number；It is protected at 28 DEG C
It deposits 10 days, then carries out being sprayed with 80ppm glutaraldehydes, continue 4min and metallic sieve is placed in hatching after spray terminates 10min
In cylinder, hatch and Spawning water 17cm is added in cylinder, knot is hatched in metallic sieve upper end not in the underwater 4cm of Spawning water after 22h
Beam, fails the ovum of hatching or the prelarva at bottom of lying prone after hatching can eliminate.
Embodiment 4
In addition to the hibernation temperature of ovum is 23 DEG C, remaining hatching method and embodiment 1 are completely the same.
Embodiment 5
In addition to the hibernation temperature of ovum is 27 DEG C, remaining hatching method and embodiment 1 are completely the same.
Comparative example 1
Spawning water preparation method is same as Example 1.
Resting egg is stored in peat soil, fish-egg is under the conditions of 23 DEG C after suspend mode 50 days, the every 3 days development feelings for checking ovum
Condition；The 15th day after resting egg all golden eyelet occurs, by resting egg taking-up be placed in metallic sieve, after metallic sieve is set
In the hatching cylinder that specification is length, width and height 50cm × 30cm × 25cm, hatch and Spawning water 15cm is added in cylinder, on metallic sieve
3cm in underwater is held, through 6h, part ovum can hatch, and grit hole is tilted to side, the prelarva that do not swim out of is made to dissociate sieve；It will
Do not hatch egg white water rinse, drain, recycling in freshness protection package sealing and it is labelled, indicate fingerling, just incubate date, ovum grain number；
After being preserved 11 days at 27 DEG C, metallic sieve is placed in former hatching cylinder hatches again, after for 24 hours, unhatched ovum is returned again
It receives and preserves, repeat above-mentioned incubation step, carry out 4 hatchings altogether.
Comparative example 2
In addition to the hibernation temperature of ovum is 25 DEG C, remaining hatching method and comparative example 1 are completely the same.
Comparative example 3
In addition to the hibernation temperature of ovum is 27 DEG C, remaining hatching method and comparative example 1 are completely the same.
600, the black pearl Medaka fish-egg with age in days is chosen, is divided into two groups totally 6 parts, 100 every part, 3 parts of 1 group press comparison
Example 1-3 modes are hatched, and every part hatches 4 times, and 3 parts of 2 groups are hatched by 1,4,5 mode of embodiment, and every part hatches 2 times, hatch results
It is shown in Table 1.
Table 1：The mode that black pearl Medaka fish tradition fish-egg preserves, hatching pattern and this patent are taken carries out the knot of contrast test
Fruit
According to table 1 as can be seen that the hatching method hatching number of the present invention is the half of conventional method, total hatching rate is than passing
System method improves about 30%, and larvae survival rate improves 10% or more, significantly improves the hatching of black pearl Medaka fish resting egg
Success rate saves brooding time and cost of labor, and being one kind being beneficial to commercialized hatching method.
Raw materials used in the present invention, equipment is unless otherwise noted the common raw material, equipment of this field；In the present invention
Method therefor is unless otherwise noted the conventional method of this field.
The above is only presently preferred embodiments of the present invention, is not imposed any restrictions to the present invention, every according to the present invention
Technical spirit still falls within the technology of the present invention side to any simple modification, change and equivalent transformation made by above example
The protection domain of case.
Claims (10)
1. a kind of hatching method of black pearl Medaka fish resting egg, it is characterised in that：The method includes the steps of：
1）The preservation of resting egg：Resting egg is preserved using no earthwork formula, fish-egg after suspend mode 50 days, checks under the conditions of 23-27 DEG C
The developmental state of ovum；
2）The pretreatment of resting egg：When there is resting egg golden eyelet occur, storage temperature is increased 1-2 DEG C, when all suspend mode
Ovum all occurs the 14-16 days after golden eyelet, and resting egg taking-up is placed in metallic sieve, is sprayed to ovum with Spawning water
Mist；
3）Lower water hatching：Metallic sieve is placed in hatching cylinder by spraying after terminating 18-22min, hatches in cylinder and Spawning water is added
13-17cm, metallic sieve upper end 2-4cm in underwater, through 5.5-6.5h, part ovum can hatch, and grit hole is tilted to side,
The prelarva that do not swim out of is set to dissociate sieve；
4）Do not hatch ovum two to incubate：Ovum clear water will not hatched to rinse, drain, be placed on and carry on ovum material, 10- is preserved at 27-28 DEG C
12 days, then carry out drug spray, the hatching of lower water.
2. the hatching method of black pearl Medaka fish resting egg as described in claim 1, which is characterized in that the step 1）Middle no soil
Mode is that resting egg is sealed in by the hermetic bag of biochemical cotton and preservative film package.
3. the hatching method of black pearl Medaka fish resting egg as claimed in claim 1 or 2, which is characterized in that the step 1）In
Check that the developmental state of ovum is to be checked once per 2-3 days.
4. the hatching method of black pearl Medaka fish resting egg as described in claim 1, which is characterized in that the step 2）Middle hatching
It is with the preparation method of water：9-11wt% peat soils leachate and 20-30ppm glutaraldehydes, water temperature 26.5-27.5 are added in water
DEG C, with biochemical cotton filter circulation filter 23 day.
5. the hatching method of black pearl Medaka fish resting egg as described in claim 1 or 4, which is characterized in that the step 2）In
Spray method is：Metallic sieve is placed in the hatching cylinder water surface or more, sprays and continues 4.5-5.5min, spray again every 18-22min
4.5-5.5min。
6. the hatching method of black pearl Medaka fish resting egg as described in claim 1, which is characterized in that the step 3）Middle hatching
The specification of cylinder is length, width and height（45-55）cm×（25-35）cm×（20-30）cm.
7. the hatching method of black pearl Medaka fish resting egg as described in claim 1, which is characterized in that the step 4）Middle load ovum
Material is the black dense form biochemistry cotton impregnated, extracted in the peat soil leachate of the methylene blue containing 4-6ppm.
8. the hatching method of black pearl Medaka fish resting egg as claimed in claim 1 or 7, which is characterized in that the step 4）In
The store method for not hatching ovum is to fall ovum in carrying on ovum material, will carry ovum material and rolls, is sealed in freshness protection package and stick mark
Label indicate fingerling, just incubate date, ovum grain number.
9. the hatching method of black pearl Medaka fish resting egg as claimed in claim 1 or 7, which is characterized in that the step 4）In
Drug spraying method is to be sprayed with 80-120ppm glutaraldehydes, continues 2-4min.
10. the hatching method of black pearl Medaka fish resting egg as claimed in claim 1 or 7, which is characterized in that the step 4）In
Lower water hatching method is that metallic sieve is placed in hatching cylinder, hatches and Spawning water 13- is added in cylinder after spray terminates 10min
17cm, metallic sieve upper end are hatched after 22-26h and are terminated not in the underwater 2-4cm of Spawning water, fail the ovum of hatching or hatch
The prelarva at bottom of lying prone afterwards can eliminate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810237523.6A CN108522371B (en) | 2018-03-21 | 2018-03-21 | Hatching method for dormant eggs of black pearl killifish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810237523.6A CN108522371B (en) | 2018-03-21 | 2018-03-21 | Hatching method for dormant eggs of black pearl killifish |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108522371A true CN108522371A (en) | 2018-09-14 |
| CN108522371B CN108522371B (en) | 2021-05-28 |
Family
ID=63484468
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810237523.6A Active CN108522371B (en) | 2018-03-21 | 2018-03-21 | Hatching method for dormant eggs of black pearl killifish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108522371B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102273415A (en) * | 2011-06-01 | 2011-12-14 | 福建省水产研究所 | Method and device for keeping germ cell of killifish alive |
| CN102396426A (en) * | 2010-09-17 | 2012-04-04 | 郭明忠 | System for automatically hatching roes |
| CN102440211A (en) * | 2011-11-17 | 2012-05-09 | 浙江海洋学院 | Killifish culture method |
| CN203897035U (en) * | 2014-01-27 | 2014-10-29 | 北京市通州鑫淼水产总公司 | Adhesive roe distributing device |
| RU2579205C1 (en) * | 2014-12-22 | 2016-04-10 | Федеральное государственное бюджетное научное учреждение "Государственный научно-производственный центр рыбного хозяйства" (ФГБНУ "Госрыбцентр") | Installation incubation of fish eggs |
-
2018
- 2018-03-21 CN CN201810237523.6A patent/CN108522371B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102396426A (en) * | 2010-09-17 | 2012-04-04 | 郭明忠 | System for automatically hatching roes |
| CN102273415A (en) * | 2011-06-01 | 2011-12-14 | 福建省水产研究所 | Method and device for keeping germ cell of killifish alive |
| CN102440211A (en) * | 2011-11-17 | 2012-05-09 | 浙江海洋学院 | Killifish culture method |
| CN203897035U (en) * | 2014-01-27 | 2014-10-29 | 北京市通州鑫淼水产总公司 | Adhesive roe distributing device |
| RU2579205C1 (en) * | 2014-12-22 | 2016-04-10 | Федеральное государственное бюджетное научное учреждение "Государственный научно-производственный центр рыбного хозяйства" (ФГБНУ "Госрыбцентр") | Installation incubation of fish eggs |
Non-Patent Citations (2)
| Title |
|---|
| 叶键 等主编: "《养鱼金典 家养热带鱼111问》", 31 July 2012, 福建科学技术出版社 * |
| 林龙 等: "鱼之梦—访上海鳉鱼玩家胡栋先生", 《水族世界》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108522371B (en) | 2021-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20210227808A1 (en) | Device and method for fish reproduction, hatching and larval culture | |
| CN109220904A (en) | The breeding of oriental cherry Thailand lion goldfish and selection | |
| CN104365557B (en) | A kind of Cryptolaemus montrouzieri artificial breeding, conservation and propagation production method | |
| Banks et al. | Age‐linked changes in salinity tolerance of larval spotted seatrout (Cynoscion nebulosus, Cuvier) | |
| Rowland | Development of techniques for the large-scale rearing of the larvae of the Australian freshwater fish golden perch, Macquaria ambigua (Richardson, 1845) | |
| Ramofafia et al. | Spawning and early larval rearing of Holothuria atra | |
| CN108293924A (en) | A kind of artificial fecundation method of Ptychobarbus dipogon | |
| Hugie et al. | The response of the tidepool sculpin, Oligocottus maculosus, to chemical stimuli from injured conspecifics, alarm signalling in the Cottidae (Pisces) | |
| Jungwirth | Some notes to the farming and conservation of the Danube salmon (Hucho hucho) | |
| Davis et al. | Spawning characteristics of the South African mudcrab Scylla serrata (Forskål) in captivity | |
| Fonds et al. | Influence of temperature and salinity on embryonic development, larval growth and number of vertebrae of the garfish, Belone belone | |
| Verreth et al. | The effect of biomanipulation of the zooplankton on the growth, feeding and survival of pikeperch (Stizostedion lucioperca) in nursing ponds | |
| KR101978417B1 (en) | A hatching method of lugworm | |
| Bearden | Salt-water impoundments for game fish in South Carolina | |
| Maslova | Problems and achievements in seed production of the Black Sea turbot in Russia | |
| CN108522371A (en) | A kind of hatching method of black pearl Medaka fish resting egg | |
| Kungvankij | Overview of penaeid shrimp culture in Asia. | |
| CN110074019A (en) | A kind of fugu obscurus, knife long-tailed anchovy and the cultivation of soft-shelled turtle stereo ecological method | |
| Heinen | An introduction to culture methods for larval and postlarval penaeid shrimp | |
| Van Zon | Studies on the biological control of aquatic weeds in The Netherlands | |
| CN111149742A (en) | Preparation and culture method of Thalictrum aquilegifolium larvae | |
| Kuo | A review of induced breeding of milkfish | |
| STEVENS | Striped bass culture in the United States | |
| CN107549094A (en) | A kind of wood frog method for breeding | |
| CN111194703B (en) | Erythroculter ilishaeformis fry breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |