CN102273415A - Method and device for keeping germ cell of killifish alive - Google Patents
Method and device for keeping germ cell of killifish alive Download PDFInfo
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- CN102273415A CN102273415A CN2011101461815A CN201110146181A CN102273415A CN 102273415 A CN102273415 A CN 102273415A CN 2011101461815 A CN2011101461815 A CN 2011101461815A CN 201110146181 A CN201110146181 A CN 201110146181A CN 102273415 A CN102273415 A CN 102273415A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses a method and device for keeping sperm cells of killifish alive. The method comprises the following steps of: P10, placing the sperm cell of the killifish into moist peat soil, then placing the moist peat soil into a temperature-controlled live-preservation box; P20, reducing temperature in the live-preservation box to 18 +/-0.5 DEG C at the speed of 2+/-0.5 DEG C per day; P30, maintaining the temperature in the live-preservation box to be 18 +/- 0.5 DEG C for 28-32 days and then executing a step P40; and P40, rising the temperature in the live-preservation box to 26+/-0.5 DEG C at the speed of 2+/-0.5 DEG C per day. The method and device for keeping the sperm cells of the killifish alive disclosed by the invention has the advantage that out-of-water survival time and survival rate of the sperm cell of killifish can be improved, so that market value of the sperm cells of the killifish is greatly improved.
Description
Technical field
The present invention relates to a kind of Medaka fish fertilized egg keepalive method and device.
Background technology
Oviparity Medaka fish has very charming body colour mostly, can be described as only a few in the fresh water pet fish and can give birth to one of fingerling that the coral fish compares favourably with the sea.Add their small and exquisite build, active active personality, being placed in the pasture and water cylinder is born perfect match really.In addition, most of oviparity Medaka fishes are raised easily to the water quality wide adaptability.These characteristics make them become one of extremely welcome tropical ornamental fish kind abroad.Especially the fertilized egg of oviparity Medaka fish has a characteristic, promptly can the dormancy certain hour, and therefore, market sale mainly is a fish-egg.But oviparity Medaka fish is extremely special on reproductive behavior: lay eggs repeatedly, and uncertain in time amount few (generally at most tens pieces, one or two piece at least); The fish-egg holding time is short, preserves usually and only survives 50% in one month.These reasons all cause natural world oviparity Medaka fish crop few, and some species has been rare in the world endangered species.All there is oviparity Medaka fish association in many countries, because output is few, fish-egg and fish all are to sell in the auction that association holds generally only.In recent years, get up by the also many of online auction.The price of oviparity Medaka fish generally be 10 to 20 dollars a pair of, the price of fish-egg is 10 dollar 10 to 30.The rare variety that idol has the player that oneself is had more transfers businessman, as long as one occur on market, even high surprising of price also can be robbed to the last pin.On the market, be just to be found in recent years because oviparity Medaka fish has a large amount in variety at home, China does not also have oviparity Medaka fish association in addition, is subjected to the restriction of customs's pertinent regulations, is difficult to personally from buying fingerling abroad.Some importers cost an arm and a leg because of it, and introduction also is reluctant to take chances.So the domestic people that can be familiar with this class fish has been few, can get the few especially if star of fingerling raiser morning.These beautiful figures are just can not see in most of cities at home at all.Just in the Xiamen, other big cities such as Shanghai, Guangzhou, Beijing, can see a spot of several kind, 4～5 centimeters long fishes, its price is also all a pair of more than 100.
The fertilized egg of viewing and admiring class oviparity Medaka fish all has the vast market prospect for sales at home and abroad, and its main subject of a sale is students in middle and primary schools, and purposes is a science subject biologic test material.Exist the preservation keep-alive phase of ovum short at present; 50% survival was only arranged after 1 month; the ovum that the period of slack sales collects can't be saved in the busy season; supply falls short of demand to cause the busy season; in addition; existing storage life does not satisfy the market circulation process need yet, has restricted the developing and the industry development of viewing and admiring class oviparity Medaka fish market.Therefore, the dried up keep-alive techniques of viewing and admiring class oviparity Medaka fish fertilized egg becomes views and admires the core technology difficult problem that class oviparity Medaka fish is sold circulation, must be researched and solved.
Summary of the invention
The invention provides a kind of Medaka fish fertilized egg keepalive method and device, it has overcome the existing deficiency of a kind of Medaka fish fertilized egg keepalive method in the background technology.
One of technical scheme that is adopted that the present invention solves its technical problem is:
A kind of Medaka fish fertilized egg keepalive method, it comprises:
P10 is used for the Medaka fish fertilized egg is put into the peat soil of preserving moisture, and the peat soil of will preserving moisture is again put into temperature adjusting keep-alive case;
P20 is used to make keep-alive the temperature inside the box to be cooled to 18 ± 0.5 ℃ 2 ± 0.5 ℃ of every days;
P30 is used to make keep-alive the temperature inside the box to remain on 18 ± 0.5 ℃, and keeps constant temperature spend 28-32 days execution in step P40 then;
P40 is used to make keep-alive the temperature inside the box to be warming up to 26 ± 0.5 ℃ 2 ± 0.5 ℃ of every days.
Among one preferred embodiment: also comprise behind this step P40:
P50 is used to make keep-alive the temperature inside the box to remain on 26 ± 0.5 ℃, and keeps constant temperature spend 28-32 days execution in step P60 then;
P60 is used to make keep-alive the temperature inside the box to be cooled to 18 ± 0.5 ℃ 2 ± 0.5 ℃ of every days.
Among one preferred embodiment: also comprise behind this step P60:
P70 is used to make keep-alive the temperature inside the box to remain on 18 ± 0.5 ℃, and keeps constant temperature spend 85-95 days execution in step P80 then;
P80 is used to make keep-alive the temperature inside the box to be warming up to 28 ± 0.5 ℃ 2 ± 0.5 ℃ of every days.
Among one preferred embodiment: this P10 comprises:
P111 is used for the alcohol disinfecting 8-12 second with Medaka fish fertilized egg usefulness 60-80%;
P112 is used for the deionized water that the Medaka fish fertilized egg is put into after the sterilization is soaked 25-35 second;
P113 is used for the Medaka fish fertilized egg is picked up, and evenly puts into the peat soil of preserving moisture again, and deposits 6-8 days in 26 ± 0.5 ℃ of environment of temperature.
Among one preferred embodiment: this P10 also comprises:
P121 is used for taking out the Medaka fish fertilized egg from peat soil, removes dead ovum;
P122, being used to adopt temperature is the alcohol disinfecting viable eggs 8-12 second of 26 ± 0.5 ℃ 60-80%;
P123 is used for 26 ± 0.5 ℃ of deionized waters that viable eggs is put into after the sterilization are soaked 25-35 second;
P124 is used for the Medaka fish fertilized egg is picked up, and then evenly puts into the peat soil of preserving moisture, and puts into the keep-alive case again and preserves.
The present invention solve its technical problem the technical scheme that is adopted two be:
A kind of Medaka fish fertilized egg keep-alive device, it comprises:
One casing (100), it has a cavity (110), a housing, that surrounds cavity (110) is located at housing with the opening of turnover cavity (110) and the door body of an adaptive opening, the housing energy heat-insulation and heat-preservation of this casing (100);
One cold and hot crosspoint, it is installed in casing (100) can control casing (100) cavity (110) temperature;
One controller (200), it is installed in casing (100) and connects cold and hot crosspoint to control cold and hot crosspoint work;
One power supply or power interface (300), its installing casing (100) and connection controller (200).
Among one preferred embodiment: this cold and hot crosspoint comprises that an interior cool-heat-exchanger (410) and that is located at cavity (110) and is abutted against inner walls is located at the outer cool-heat-exchanger (420) of housing outer wall.
Among one preferred embodiment: this cavity is established temperature controller in (110), and this temperature controller connects controller (200).
Among one preferred embodiment: cool-heat-exchanger (410) comprises that a diapire and extends upward the perisporium that forms by the diapire periphery in this.
The technical program is compared with background technology, and it has following advantage:
1, can improve dried up time-to-live of Medaka fish fertilized egg and survival rate, to improve the market value of Medaka fish fertilized egg greatly;
2, the Medaka fish fertilized egg adopts in alcohol disinfecting, the deionized water and soaks, and can improve dried up time-to-live of Medaka fish fertilized egg and survival rate;
3, make the dried up time-to-live of Medaka fish fertilized egg bring up to survival in 6 months below 50% more than 70% from survival in average 1 month;
4, Medaka fish fertilized egg keep-alive device comprises casing, cold and hot crosspoint, controller, can control variation of temperature in the cavity on request.
Description of drawings
The invention will be further described below in conjunction with drawings and Examples.
Fig. 1 has illustrated the schematic diagram of the control keep-alive device of a preferred embodiment.
Fig. 2 has illustrated the time of keepalive method of a preferred embodiment and the schematic diagram of temperature.
Embodiment
Please refer to Fig. 1, a kind of Medaka fish fertilized egg keep-alive device, it comprises a casing 100, one cold and hot crosspoint, a controller 200 and a power supply or power interface 300.
This casing 100, it has a cavity 110, a housing, that surrounds cavity 110 is located at housing with the opening of turnover cavity 110 and the door body of an adaptive opening.The housing energy heat-insulation and heat-preservation of this casing 100, among the present embodiment, this housing self has the heat-insulation and heat-preservation function, or this housing self does not have the heat-insulation and heat-preservation function and the inner walls lining is established insulating, and this heat-insulation and heat-preservation material is selected acrylic board etc. for use.
This cold and hot crosspoint, it is installed in casing 100 can control casing 100 cavitys 110 temperature.This cold and hot crosspoint comprises that an interior cool-heat-exchanger 410 and that is located at cavity 110 and is abutted against inner walls is located at the outer cool-heat-exchanger 420 on limit, housing outer wall left side, and it can improve in the cavity temperature or reduce temperature in the cavity.Among the present embodiment, adopt to lay respectively at the inside and outside inside and outside cool-heat-exchanger of heat-insulation layer, can accurately control cavity temperature.Among the present embodiment, cool-heat-exchanger 410 comprises that a diapire and extends upward the perisporium that forms by the diapire periphery in this.
This controller 200, it is installed in the limit, left side of casing 100 and connects interior cool-heat-exchanger 410 and outer cool-heat-exchanger 420, to control cold and hot crosspoint work, with control cavity 110 temperature.
This power supply or power interface 300, its installing casing limit, 100 left side and connection controller 200.Among the present embodiment, described controller 200, outer cool-heat-exchanger, power supply or power interface 300 upper and lower settings.
Among the present embodiment, establish temperature controller in this cavity 110, this temperature controller connects controller 200.
A kind of Medaka fish fertilized egg keepalive method, it comprises:
P10 is used for the Medaka fish fertilized egg is put into the peat soil of preserving moisture, and the peat soil of will preserving moisture is again put into temperature adjusting keep-alive case;
P20 is used to make keep-alive the temperature inside the box to be cooled to 18 ± 0.5 ℃ 2 ± 0.5 ℃ of every days; Among the present embodiment, the cold and hot crosspoint work of controller 200 controls is with control keep-alive the temperature inside the box;
P30 is used to make keep-alive the temperature inside the box to remain on 18 ± 0.5 ℃, and keeps constant temperature spend 28-32 days execution in step P40 then;
P40 is used to make keep-alive the temperature inside the box to be warming up to 26 ± 0.5 ℃ 2 ± 0.5 ℃ of every days;
P50 is used to make keep-alive the temperature inside the box to remain on 26 ± 0.5 ℃, and keeps constant temperature spend 28-32 days execution in step P60 then;
P60 is used to make keep-alive the temperature inside the box to be cooled to 18 ± 0.5 ℃ 2 ± 0.5 ℃ of every days;
P70 is used to make keep-alive the temperature inside the box to remain on 18 ± 0.5 ℃, and keeps constant temperature spend 85-95 days execution in step P80 then;
P80 is used to make keep-alive the temperature inside the box to be warming up to 28 ± 0.5 ℃ 2 ± 0.5 ℃ of every days, unpacks, and selects mature egg packing shipment.
Among the present embodiment: this P10 comprises:
P111 is used for the alcohol disinfecting 8-12 second with Medaka fish fertilized egg usefulness 60-80% weight concentration;
P112 is used for the deionized water that the Medaka fish fertilized egg is put into after the sterilization is soaked 25-35 second;
P113 is used for the Medaka fish fertilized egg is picked up, and evenly puts into the peat soil of preserving moisture again, and deposits 6-8 days in 26 ± 0.5 ℃ of environment of temperature;
P121 is used for taking out the Medaka fish fertilized egg from peat soil, removes dead ovum;
P122, being used to adopt temperature is the alcohol disinfecting viable eggs 8-12 second of 26 ± 0.5 ℃ 60-80%;
P123 is used for 26 ± 0.5 ℃ of deionized waters that viable eggs is put into after the sterilization are soaked 25-35 second;
P124 is used for the Medaka fish fertilized egg is picked up, and then evenly puts into the peat soil of preserving moisture, and puts into the keep-alive case again and preserves.
Adopt this method, can make the dried up time-to-live of Medaka fish fertilized egg bring up to survival in 6 months below 50% more than 70% from survival in average 1 month.
Among the present embodiment, described " peat soil ", its English name: peat soil, it is: in some river lake deposit low plain and intermountain valley floor, because long-term ponding, aquatic vegetation is dense, under anaerobic conditions, the soil that decomposes inadequate plant residue accumulation and formation peat horizon in a large number.
The above, only for preferred embodiment of the present invention, so can not limit scope of the invention process according to this, i.e. the equivalence of doing according to claim of the present invention and description changes and modification, all should still belong in the scope that the present invention contains.
Claims (9)
1. Medaka fish fertilized egg keepalive method, it is characterized in that: it comprises:
P10 is used for the Medaka fish fertilized egg is put into the peat soil of preserving moisture, and the peat soil of will preserving moisture is again put into temperature adjusting keep-alive case;
P20 is used to make keep-alive the temperature inside the box to be cooled to 18 ± 0.5 ℃ 2 ± 0.5 ℃ of every days;
P30 is used to make keep-alive the temperature inside the box to remain on 18 ± 0.5 ℃, and keeps constant temperature spend 28-32 days execution in step P40 then;
P40 is used to make keep-alive the temperature inside the box to be warming up to 26 ± 0.5 ℃ 2 ± 0.5 ℃ of every days.
2. a kind of Medaka fish fertilized egg keepalive method according to claim 1 is characterized in that: also comprise behind this step P40:
P50 is used to make keep-alive the temperature inside the box to remain on 26 ± 0.5 ℃, and keeps constant temperature spend 28-32 days execution in step P60 then;
P60 is used to make keep-alive the temperature inside the box to be cooled to 18 ± 0.5 ℃ 2 ± 0.5 ℃ of every days.
3. a kind of Medaka fish fertilized egg keepalive method according to claim 2 is characterized in that: also comprise behind this step P60:
P70 is used to make keep-alive the temperature inside the box to remain on 18 ± 0.5 ℃, and keeps constant temperature spend 85-95 days execution in step P80 then;
P80 is used to make keep-alive the temperature inside the box to be warming up to 28 ± 0.5 ℃ 2 ± 0.5 ℃ of every days.
4. according to claim 1 or 2 or 3 described a kind of Medaka fish fertilized egg keepalive methods, it is characterized in that: this P10 comprises:
P111 is used for the alcohol disinfecting 8-12 second with Medaka fish fertilized egg usefulness 60-80%;
P112 is used for the deionized water that the Medaka fish fertilized egg is put into after the sterilization is soaked 25-35 second;
P113 is used for the Medaka fish fertilized egg is picked up, and evenly puts into the peat soil of preserving moisture again, and deposits 6-8 days in 26 ± 0.5 ℃ of environment of temperature.
5. a kind of Medaka fish fertilized egg keepalive method according to claim 4, it is characterized in that: this P10 also comprises:
P121 is used for taking out the Medaka fish fertilized egg from peat soil, removes dead ovum;
P122, being used to adopt temperature is the alcohol disinfecting viable eggs 8-12 second of 26 ± 0.5 ℃ 60-80%;
P123 is used for 26 ± 0.5 ℃ of deionized waters that viable eggs is put into after the sterilization are soaked 25-35 second;
P124 is used for the Medaka fish fertilized egg is picked up, and then evenly puts into the peat soil of preserving moisture, and puts into the keep-alive case again and preserves.
6. Medaka fish fertilized egg keep-alive device, it is characterized in that: it comprises:
One casing (100), it has a cavity (110), a housing, that surrounds cavity (110) is located at housing with the opening of turnover cavity (110) and the door body of an adaptive opening, the housing energy heat-insulation and heat-preservation of this casing (100);
One cold and hot crosspoint, it is installed in casing (100) can control casing (100) cavity (110) temperature;
One controller (200), it is installed in casing (100) and connects cold and hot crosspoint to control cold and hot crosspoint work;
One power supply or power interface (300), its installing casing (100) and connection controller (200).
7. a kind of Medaka fish fertilized egg keep-alive device according to claim 6 is characterized in that: this cold and hot crosspoint comprises that an interior cool-heat-exchanger (410) and that is located at cavity (110) and is abutted against inner walls is located at the outer cool-heat-exchanger (420) of housing outer wall.
8. a kind of Medaka fish fertilized egg keep-alive device according to claim 6, it is characterized in that: this cavity is established temperature controller in (110), and this temperature controller connects controller (200).
9. a kind of Medaka fish fertilized egg keep-alive device according to claim 7 is characterized in that: cool-heat-exchanger (410) comprises that a diapire and extends upward the perisporium that forms by the diapire periphery in this.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101461815A CN102273415B (en) | 2011-06-01 | 2011-06-01 | Method for keeping germ cell of killifish alive |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101461815A CN102273415B (en) | 2011-06-01 | 2011-06-01 | Method for keeping germ cell of killifish alive |
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| CN102273415A true CN102273415A (en) | 2011-12-14 |
| CN102273415B CN102273415B (en) | 2013-01-09 |
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| CN2011101461815A Expired - Fee Related CN102273415B (en) | 2011-06-01 | 2011-06-01 | Method for keeping germ cell of killifish alive |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522371A (en) * | 2018-03-21 | 2018-09-14 | 浙江师范大学 | A kind of hatching method of black pearl Medaka fish resting egg |
| CN111758625A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for industrial in-vitro breeding of procambarus clarkii |
| CN111758626A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for controlling development of in-vitro fertilized egg embryo of procambarus clarkii |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU789063A1 (en) * | 1978-01-09 | 1980-12-23 | Московский Ордена Ленина И Ордена Трудового Красного Знамени Государственный Университет Им.М.В. Ломоносова | Method of improving vital capacity of impregnated fish spawn |
| EP0710439B1 (en) * | 1994-10-31 | 2002-05-29 | National Agricultural Research Organization | Method for cryopreservation of primordial germ cells and germ cells |
| CN1552200A (en) * | 2003-05-29 | 2004-12-08 | 中国水产科学研究院黄海水产研究所 | Glass freezing preserving method for fish embryo |
| CN1593124A (en) * | 2003-09-10 | 2005-03-16 | 中国科学院海洋研究所 | Red sea bream embryo freezing preservation method |
| CN201520481U (en) * | 2009-10-27 | 2010-07-07 | 沈新华 | Storage box with dual functions of refrigeration and heating |
| CN202095400U (en) * | 2011-06-01 | 2012-01-04 | 福建省水产研究所 | Fertilized egg keep-alive device for killifishes |
-
2011
- 2011-06-01 CN CN2011101461815A patent/CN102273415B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU789063A1 (en) * | 1978-01-09 | 1980-12-23 | Московский Ордена Ленина И Ордена Трудового Красного Знамени Государственный Университет Им.М.В. Ломоносова | Method of improving vital capacity of impregnated fish spawn |
| EP0710439B1 (en) * | 1994-10-31 | 2002-05-29 | National Agricultural Research Organization | Method for cryopreservation of primordial germ cells and germ cells |
| CN1552200A (en) * | 2003-05-29 | 2004-12-08 | 中国水产科学研究院黄海水产研究所 | Glass freezing preserving method for fish embryo |
| CN1593124A (en) * | 2003-09-10 | 2005-03-16 | 中国科学院海洋研究所 | Red sea bream embryo freezing preservation method |
| CN201520481U (en) * | 2009-10-27 | 2010-07-07 | 沈新华 | Storage box with dual functions of refrigeration and heating |
| CN202095400U (en) * | 2011-06-01 | 2012-01-04 | 福建省水产研究所 | Fertilized egg keep-alive device for killifishes |
Non-Patent Citations (4)
| Title |
|---|
| JONATHAN R. MATIAS ET AL: "The survival of embryos of the annual fish nothobranchius grentheri exposed to temperature extremes and the subsequent effects on embryonic diapause", 《JOURNAL OF EXPERIMENTAL ZOOLOGY.PART A:ECOLOGICAL GENETICS AND PHYSIOLOGY》 * |
| JULES MARKOFSKY ET AL: "The effect of temperature and season of collection on the onset and duration of diapause in embryos of the annual fish nothobranchius guentheri", 《JOURNAL OF EXPERIMENTAL ZOOLOFY.PART A:ECOLOGICAL GENETICS AND PHYSIOLOGY》 * |
| 刘长水 等: "温度对贡式假腮鳉的生长发育、性成熟和成活的影响", 《鲁东大学学报(自然科学版)》 * |
| 张岩 等: "海洋贝类配子及胚胎的低温冷冻保存", 《海洋水产研究》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522371A (en) * | 2018-03-21 | 2018-09-14 | 浙江师范大学 | A kind of hatching method of black pearl Medaka fish resting egg |
| CN108522371B (en) * | 2018-03-21 | 2021-05-28 | 浙江师范大学 | Hatching method for dormant eggs of black pearl killifish |
| CN111758625A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for industrial in-vitro breeding of procambarus clarkii |
| CN111758626A (en) * | 2020-06-29 | 2020-10-13 | 中国水产科学研究院淡水渔业研究中心 | Method for controlling development of in-vitro fertilized egg embryo of procambarus clarkii |
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| CN102273415B (en) | 2013-01-09 |
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