CN108498435A - A kind of emulsion compositions and its application in cosmetics - Google Patents
A kind of emulsion compositions and its application in cosmetics Download PDFInfo
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- CN108498435A CN108498435A CN201810407321.1A CN201810407321A CN108498435A CN 108498435 A CN108498435 A CN 108498435A CN 201810407321 A CN201810407321 A CN 201810407321A CN 108498435 A CN108498435 A CN 108498435A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/981—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of mammals or bird
- A61K8/985—Skin or skin outgrowth, e.g. hair, nails
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
- A61K8/26—Aluminium; Compounds thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
- A61K8/375—Esters of carboxylic acids the alcohol moiety containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
- A61K2800/30—Characterized by the absence of a particular group of ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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Abstract
The present invention provides a kind of emulsion compositions, including skin progenitor cell culture supernatant, montmorillonite, bentonite, Aloe Vera Gel, camellia oil, lecithin, glycerin monostearate；The skin progenitor cell is irradiated using low dosage ultraviolet light.Quality of the present invention is clear, has good water lock moistening effect, and the salubrious sense of skin is kept when can be long, and can improve the vigorous state of skin fat secretion, has good oil-control effect, has very notable curative effect particularly with oiliness sensitivity flesh.
Description
Technical field
The invention belongs to beauty and make-up fields, relate generally to a kind of oil control emulsion composition.
Background technology
Skin and epidermis are the significant defense barriers of human body, and skin is rich in sebaceous glands and continuous updating.Sebum is for moistening
The substance of wet epidermis, secretion are the normal phenomenons to skin beneficiating.Sebum is the natural product of sebaceous glands, and sebum is substantially
The compound of grease.Sebum protection skin also has scalp and by lubricating cuticula by skin radiance.But the mistake of sebum
Degree secretion or seborrhea can lead to aesthstic obstacle.That there are fat secretions is vigorous for Oily, and forehead, the wing of nose have glossy, pore
The problems such as coarse, cortex is thick hard rough, seriously affects external image.Ultraviolet light, from excessive physico of external environment
Learning stimulation and pressure, fatigue etc. makes the normal function of skin degenerate, and keeps skin barrier impaired, exacerbates the hair of these situations
It is raw.Wherein, from external radiation, such as ultraviolet light is the stimulus that skin is difficult to avoid, and is that skin various problems occurs
Important factor in order.And modern society, the frequent use of various electronic products, computer, mobile phone, tablet etc., skin by micronic dust,
The erosion getting worse of radiation etc..For oily skin, sebum excessively divides under the stimulation of extraneous poor environment
It secretes, facial oiliness is not easy to clean, is repugnant to the eye；It is more serious facial skin disease also easily occur, such as acne, seborrhea
Deng.It controls sebaceous glands excessive secretion, mitigate fuel-displaced symptom, be to maintain the key of skin health.
However there is no make effective ways of the skin to antisum, radiation etc., the controls such as common suncream well at present
Oil product, often quality is thick and heavy, and frequently multiple smearing, easily deposits excessive impurity, grease etc., pore is caused to block, influence skin
Skin eubolism keeps skin oiliness problem more serious, the more controls the more oily；And the ingredients such as sun-screening agent contained therein, alcohol increase
The insecurity such as irritation, light sensitivity improve the probability that skin generates condition susceptible, make skin damage, occur it is coarse,
Play pimple, the symptoms such as itch.
Invention content
The purpose of the present invention is to provide one kind being used for oil control emulsion, and quality is frivolous, can persistently keep species activity, helps
Skin resists the environmental stimulis such as ultraviolet light, and improves the undesirable conditions such as skin greasing, sensitivity.
The purpose of the present invention is what is realized by following measures：
A kind of emulsion compositions, including skin progenitor cell culture supernatant, montmorillonite, bentonite, Aloe Vera Gel, camellia oil,
Lecithin, glycerin monostearate；The skin progenitor cell is irradiated using low dosage ultraviolet light.
Preferably, above-mentioned emulsion composition include 800-1500 parts of skin progenitor cell culture supernatants, 5-10 parts of montmorillonite,
5-10 parts of bentonite, 10-15 parts of Aloe Vera Gel, 2-15 parts of camellia oil, 1-10 parts of lecithin, 2-10 parts of glycerin monostearate.It is excellent
Choosing, the montmorillonite and bentonitic grain size are 0.25-20 microns.
Preferably, skin progenitor cell culture supernatant is the supernatant collected to skin progenitor cell culture 3 days or more.
Above-mentioned ultraviolet light is radiated at Continuous irradiation 2-24 hours after skin progenitor cell culture 6-24 hours.Preferably, the ultraviolet light shines
It is 3-7mJ/cm to penetrate dosage2.Dosage is adjusted according to irradiation time.
The present invention can improve the ability that skin resists the radiation such as environmental stimuli, especially ultraviolet light, be rich in a variety of biologies
Active material can persistently keep activity and stability；And quality of the present invention is frivolous, Oily is repeatedly smeared not
It can feel greasy.
In order to be suitable for sensitive skin, neural stem cell lysate is added in the skin progenitor cell culture medium.The god
The mixing obtained after the release of cell and intracellular matter after stem cell lysate refers to neural stem cell destruction or dissolving
Object.Including cytosolic fractions, cell wall fraction and metabolite etc..Preferably, the cell cracking uses freeze-thaw method；Specifically,
Neural stem cell is placed in cooling in liquid nitrogen, 35-40 DEG C melts, and is repeated 3 times above；Membrane filtration collected film object to get.It is described
Film uses the apertures 250-500nm filter membrane.
Preferably, after skin progenitor cell ultraviolet light is irradiated and cultivated, above-mentioned neural stem cell is added in the medium
Lysate.It finds in an experiment, 2-12 hours addition neural stem cell lysates after ultraviolet light irradiates, and continues to cultivate
12-48 hours, be conducive to the collaboration of various composition.
The culture medium that above-mentioned skin progenitor cell culture uses is：Basal medium DMEM/F12,25-30 μ g/LVEGF, 30-
40 μ g/L EGF, the NGF of 5-10 μ g/L, the human albumin of 5~50mg/L, 20-30 μ g/L vitamin Es, ironic citrate 1-
3mmol/L, glutamine 1-2mmol/L, gentamicin 10-100g/L, putrescine 0.8-2.0mg/L.Neural stem cell lysate
Dosage is the 0.5-2wt% of culture medium.
The present invention also provides oil control emulsions, containing above-mentioned composition and conventional cosmetic grade auxiliary agent, wherein being free of wine
Essence, essence or preservative.
It is used for application of the composition in preparing anti-spoke, sun-proof or oil-control cosmetic the present invention also provides above-mentioned.
Advantageous effect
1. quality of the present invention is clear, there is good water lock moistening effect, the salubrious sense of skin is kept when can be long, and
The vigorous state of skin fat secretion can be improved, there is good oil-control effect, have particularly with oiliness sensitivity flesh very aobvious
The effect of work.
2. the present invention improves the defense function of skin Antiradiation injury；It is used in the quick of external boundary's stimulation extreme sensitivity
Feel flesh, can also there is good radioresistance effect.The various active ingredients of the present invention are synergistic each other, enhance skin barrier function,
The immunity of damaged skin is improved, a variety of symptoms such as skin sensitivity, greasy are improved.
3. emulsion-stabilizing of the present invention is rich in a variety of active ingredients, activity can be kept well before and after use and stablized
Property.The present invention, which does not add the poisonous and harmful substances such as preservative, pigment, essence, can have excellent radioresistance, repair oiliness sensitivity flesh
The effect of skin, safe, non-stimulated, have no toxic side effect, quality is frivolous, long-term, frequently use will not cause skin to bear and tire out
Meter injury.
Description of the drawings
Fig. 1 bioactivity detects
Specific implementation mode
The present invention is specifically described below by embodiment, it is necessary to which indicated herein to be the present embodiment can only use
In invention is further explained, it should not be understood as limiting the scope of the invention, the people that is skilled in technique in the field
Member can make some nonessential modifications and adaptations according to the content of aforementioned present invention.Oil control emulsion of the present invention meets
《Cosmetics safety technical specification》.
Embodiment 1
A kind of oil control emulsion, including following preparation process：
1. the preparation of neural stem cell lysate
Human nerve stem cell is placed in liquid nitrogen cooling 30min, melts in 37 DEG C of cell incubators, is repeated 3 times；250nm
Aperture membrane filtration collected film object to get.It is spare to be put into 37 DEG C of heat preservations.
2. the preparation of skin progenitor cell culture supernatant：
(1) culture medium is configured：Basal medium be DMEM/F12,25 μ g/L VEGF, 35 μ g/L EGF, 8 μ g/L NGF,
The human albumin of 20mg/L, 25 μ g/L vitamin Es, ironic citrate 2mmol/L, glutamine 2mmol/L, gentamicin 50g/
L, putrescine 1.6mg/L.
(2) it will be cultivated in application on human skin stem cell step (1) culture medium, 37 DEG C are cultivated 12 hours, and 5mJ/cm is then used2It is ultraviolet
Line Continuous irradiation cell 18 hours continues to cultivate 6h after irradiation.
(3) neural stem cell lysate made from 1wt% steps 1 is added in the medium, is further cultured for for 24 hours, then collecting
Cell liquid, centrifugation, takes supernatant, the apertures 300nm membrane filtration to get.
3. the preparation of oil control emulsion
12 parts of Aloe Vera Gel is uniformly mixed for 5 parts with camellia oil, then sequentially adds 1200 parts of skin progenitor cell culture supernatants
2 parts of 6 parts of liquid, 8 parts of montmorillonite and bentonite, 5 parts of lecithin and glycerin monostearate, are sufficiently mixed and stir and evenly mix, homogeneous；
40 DEG C keep the temperature 2 hours, are placed in 4 DEG C of household freezers and preserve.
Unless otherwise instructed, number of the present invention is in terms of mass fraction.
Embodiment 2
A kind of oil control emulsion, including following preparation process：
1. the preparation of neural stem cell lysate
Human nerve stem cell is placed in liquid nitrogen cooling 30min, melts in 37 DEG C of cell incubators, is repeated 4 times；300nm
Aperture membrane filtration collected film object to get.It is spare to be put into 37 DEG C of heat preservations.
2. the preparation of skin progenitor cell culture supernatant：
(1) culture medium is configured：Basal medium be DMEM/F12,28 μ g/L VEGF, 28 μ g/L EGF, 8 μ g/L NGF,
The human albumin of 20mg/L, 25 μ g/L vitamin Es, ironic citrate 2.5mmol/L, glutamine 1.6mmol/L, gentamicin
50g/L, putrescine 1.5mg/L.
(2) by application on human skin stem cell in step (1) inoculation of medium, 37 DEG C are cultivated 24 hours, and 7mJ/cm is then used2It is purple
Outside line Continuous irradiation cell 5 hours continues to cultivate 12h after irradiation.
(3) neural stem cell lysate made from 2wt% steps 1 is added in the medium, is further cultured for 36h, then collects
Cell liquid, centrifugation, takes supernatant, the apertures 500nm membrane filtration to get.
3. the preparation of oil control emulsion
10 parts of Aloe Vera Gel is uniformly mixed for 3 parts with camellia oil, 800 parts of skin progenitor cell culture supernatants are then added, cover
5 parts of de- soil and 2 parts of 5 parts of bentonite, 1 part of lecithin and glycerin monostearate, are sufficiently mixed and stir and evenly mix, homogeneous；At 40 DEG C
Heat preservation 2 hours is placed in 4 DEG C of household freezers and preserves.
Embodiment 3
A kind of oil control emulsion, including following preparation process：
1. the preparation of neural stem cell lysate
Human nerve stem cell is placed in liquid nitrogen cooling 30min, melts in 37 DEG C of cell incubators, is repeated 3 times；450nm
Aperture membrane filtration collected film object to get.It is spare to be put into 37 DEG C of heat preservations.
2. the preparation of skin progenitor cell culture supernatant：
(1) culture medium is configured：Basal medium be DMEM/F12,25 μ g/L VEGF, 40 μ g/L EGF, 5 μ g/L NGF,
The human albumin of 35mg/L, 20 μ g/L vitamin Es, ironic citrate 1mmol/L, glutamine 1.2mmol/L, gentamicin
80g/L, putrescine 1.0mg/L.
(2) it will be cultivated in application on human skin stem cell step (1) culture medium, 37 DEG C are cultivated 6 hours, and 3mJ/cm is then used2It is ultraviolet
Line Continuous irradiation cell 22 hours continues to cultivate 3h after irradiation.
(3) neural stem cell lysate made from 0.8wt% steps 1 is added in the medium, is further cultured for 45h, then receives
Collect cell liquid, centrifugation takes supernatant, the apertures 450nm membrane filtration to get.
3. the preparation of oil control emulsion
13 parts of Aloe Vera Gel is uniformly mixed for 10 parts with camellia oil, be then added 1500 parts of skin progenitor cell culture supernatants,
10 parts of montmorillonite and 5 parts of 8 parts of bentonite, 6 parts of lecithin and glycerin monostearate, are sufficiently mixed and stir and evenly mix, homogeneous；40
DEG C heat preservation 2 hours, is placed in 4 DEG C of household freezers and preserves.
Embodiment 4
One, stability of emulsion is tested：This oil control emulsion is placed under the conditions of 500 × g and centrifuges 10min, has no lamination,
Emulsion-stabilizing.
The biological activity test of two, oil control emulsions
Sample to be tested：This oil control emulsion is placed into 37 DEG C of unclosed environment 12h.
Using human skin fibroblasts strain Bal/3T3, blank control and negative control are set, are inoculated in 24 orifice plates thin
Born of the same parents' (in addition to blank group), are then added the 800 μ l of DMEM culture mediums containing 0.5% serum per hole, and 1 group of 100 μ l of addition of experiment is to be measured
Sample tests 2 groups of 50 μ l samples to be tested of addition, tests 3 groups of 25 μ l samples to be tested of addition, after being cultivated for 24 hours in incubator, MTT inspections
Survey cell proliferative conditions.The results are shown in Figure 1 for cell survival rate (proliferation rate), blank control 0.
Experimental result shows that this oil control emulsion has the bioactive substance for promoting cell Proliferation, and its activity can be lasting
It keeps, and shows dose dependent.
Three, skins improve test
1. tested crowd：
Oiliness sensitivity skin crowd tests：(1) after by smearing 10% lactic acid solution to volunteer's nose side, volunteer
Self-appraisal tingling sensation, standards of grading are 0~4 point：0 point is painless, and 1 point is slight shouting pain, and 2 points are slight shouting pain, and 3 points are moderate
Shouting pain, 4 points strong for shouting pain.The individual of moderate or strong tingling sensation thinks it with sensitive skin.
After being determined as that sensitive skin volunteer thoroughly cleans face with facial cleanser, water is dried with towel, skin can go out
Existing tight feeling；Without any oil-control product, skin conditions are observed silently, calculate the time that skin tight feeling disappears.If tight feeling exists
It disappears within 30 minutes or so after washing one's face, is neutral skin；It is Oily if tight feeling disappears after washing one's face within 20 minutes；If
Tight feeling just disappears for 40 minutes or so after washing one's face, and is dry skin.
Using the Oily screened in sensitive skin as tested crowd.Screen 21 oiliness sensitivity skin.；Wherein 12
Position is computer work crowd, before at least 8 hours or more daily are exposed to computer.
2. experimental method：
The minimum erythema value for measuring each volunteer before tested using sun protection factor detecting and analysing system, i.e., in fixed light source
With the time required to causing skin to generate just visible erythema under conditions of irradiation distance.SCH electrical measurements instrument (Skicon-200)
Measure keratoderma water content.Sebumeter SM180 (Courage＆Khazaka) measures skin sebum secretory volume.
Thereafter, this oil control emulsion is smeared after daily morning and evening cleaning face, is used continuously 12 weeks, is not used during experiment other
The oil-controls product such as moisturizing, anti-aging.
Terminate within tested 12 weeks, carry out minimum erythema value test, keratoderma water content and skin sebum secretory volume again,
Compare tested front and back difference.And it is tested after pay a return visit within 3 months, test again minimum erythema value, keratoderma water content and
Skin sebum secretory volume.
3. experimental result
(1) average time of tested crowd's minimum erythema value 7.9h before treatment rises to 13.8h, skin keratin after treatment
18.3% or more is risen before and after layer water content test, sebum secretion amount has dropped 19.7%.It of particular concern is, computer
Work subjects skin's situation has a significantly more variation, and average time of minimum erythema value, the 7.2h before treatment rose to
16.5h after treatment rises 18.9% before and after the test of keratoderma water content, and sebum secretion amount has dropped 22.9%.
(2) it pays a return visit within 3 months after tested, minimum erythema value, keratoderma water content and sebum secretion amount maintain
State at the end of tested, does not decline, and oiliness sensitivity skin-improving benefits are maintained, and are had no repeatedly.
It is oiliness sensitivity flesh before tested crowd is tested, skin water lock poor performance sensitive to environmental stimuli, fat secretion is prosperous
It contains, after treatment in 12 weeks, the time that skin generates erythema is substantially increased, and moisture content of skin rises, and sebum secretion amount declines, by
Examination person's sensitive skin is substantially increased light radiation tolerance, and not only skin light loss symptom is alleviated significantly, but also this product
Not only without any sensitization situation or exacerbation skin sensitivity situation, skin greasing, condition responsive are improved instead；Therapeutic effect simultaneously
It long-acting can keep, it will not be repeatedly.
Claims (10)
1. a kind of emulsion compositions, including skin progenitor cell culture supernatant, montmorillonite, bentonite, Aloe Vera Gel, camellia oil, ovum
Phosphatide, glycerin monostearate；The skin progenitor cell is irradiated using low dosage ultraviolet light.
2. emulsion compositions as described in claim 1, including 800-1500 parts of skin progenitor cell culture supernatants, montmorillonite 5-
10 parts, 5-10 parts of bentonite, 10-15 parts of Aloe Vera Gel, 2-15 parts of camellia oil, 1-10 parts of lecithin, glycerin monostearate 2-10
Part.
3. emulsion compositions as claimed in claim 1 or 2, after the ultraviolet light is radiated at skin progenitor cell culture 6-24 hours
Continuous irradiation 2-24 hours.
4. emulsion compositions as claimed in claim 3, the ultraviolet irradiating dose is 3-7mJ/cm2。
5. the emulsion compositions as described in claim 1-4 is any, neural stem cell is added in the skin progenitor cell culture medium
Lysate；The cell cracking uses freeze-thaw method；Specifically, neural stem cell being placed in cooling in liquid nitrogen, 35-40 DEG C melts,
It is repeated 3 times above；Membrane filtration collected film object to get.
6. emulsion compositions as claimed in claim 5, after skin progenitor cell ultraviolet light is irradiated and cultivated, in the medium
Add above-mentioned neural stem cell lysate.
7. emulsion compositions as claimed in claim 6,2-12 hours addition neural stem cell are split after ultraviolet light irradiates
Object is solved, and continues culture 12-48 hours.
8. the emulsion compositions as described in claim 1-7 is any, the culture medium that the skin progenitor cell culture uses are：Base
The human serum of basal culture medium DMEM/F12,25-30 μ g/L VEGF, 30-40 μ g/L EGF, the NGF of 5-10 μ g/L, 5~50mg/L
Albumen, 20-30 μ g/L vitamin Es, ironic citrate 1-3mmol/L, glutamine 1-2mmol/L, gentamicin 10-100g/L,
Putrescine 0.8-2.0mg/L；Neural stem cell lysate dosage is the 0.5-2wt% of culture medium.
9. a kind of lotion contains any compositions of claim 1-8 and conventional cosmetic grade auxiliary agent.
10. application of the emulsion compositions according to claims 1-8 in cosmetics.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109966160A (en) * | 2019-04-01 | 2019-07-05 | 中国日用化学研究院有限公司 | A kind of multiple radiation protection Pickering lotion and preparation method thereof containing natural products |
| CN116076365A (en) * | 2023-02-17 | 2023-05-09 | 贵州省林业学校(贵州省林业干部学校) | Rapid propagation method of Weining short-column tea-oil tree scion |
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| CN106244652A (en) * | 2016-08-17 | 2016-12-21 | 广州宏柯源生物科技有限公司 | The preparation method of a kind of human mesenchymal stem cell culture supernatant lyophilized powder and prepared lyophilized powder |
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| CN109966160A (en) * | 2019-04-01 | 2019-07-05 | 中国日用化学研究院有限公司 | A kind of multiple radiation protection Pickering lotion and preparation method thereof containing natural products |
| CN116076365A (en) * | 2023-02-17 | 2023-05-09 | 贵州省林业学校(贵州省林业干部学校) | Rapid propagation method of Weining short-column tea-oil tree scion |
| CN116076365B (en) * | 2023-02-17 | 2023-11-14 | 贵州省林业学校(贵州省林业干部学校) | Rapid propagation method of Weining short-column tea-oil tree scion |
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Application publication date: 20180907 |