CN1618981A - Dry cell secretion factor and its preparation method and application - Google Patents
Dry cell secretion factor and its preparation method and application Download PDFInfo
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- CN1618981A CN1618981A CNA200310115014XA CN200310115014A CN1618981A CN 1618981 A CN1618981 A CN 1618981A CN A200310115014X A CNA200310115014X A CN A200310115014XA CN 200310115014 A CN200310115014 A CN 200310115014A CN 1618981 A CN1618981 A CN 1618981A
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Abstract
A stem cell secretion factor used for cosmetics, health-care product and regeneration medicine to promote reproduction and differentiation of cell and preventing senility of cell is an immortal cell line generated during the reproduction and differentiation of stem cells. It contains growth factor, protein, active polypeptide and trace elements.
Description
Technical field
The present invention relates to a kind of stem cell secretion factor and preparation method thereof and the application in makeup, healthcare products and regenerative medicine.
Background technology
The adult stem cell that stem cell secretion factor is taken from human embryo stem cell and multiple embryonic tissue separation and purification acquisition (comprises hemopoietic stem cell, neural stem cell, liver stem cells, skin progenitor cell, muscle stem cell or the like), go down to posterity and build up different stem cell lines through tens of generations to the cultivation in generations up to a hundred, or (comprise Hoxb-4 through genetic modification, telomerase activation subunits etc.) immortal cell line of the source of human stem cell of setting up can secrete multiple somatomedin in the stem cells hyperplasia atomization, protein, active polypeptide, trace elements etc. promote the cell growth, activation, the material of regeneration etc.
Contain the extremely strong somatomedin of various physiological actions in the stem cell secretion factor, its effect gets most of the attention.Somatomedin is meant " cell fission activation factor ", can promote cell proliferation, differentiation, prevents cell aging.As a result, metabolism is vigorous, and old cell is constantly replaced by new cell, makes cell activation simultaneously.The research that this type of stem cell secretion factor is applied to makeup, healthcare products and regenerative medicine field does not appear in the newspapers.
Summary of the invention
The objective of the invention is: 1, provide a kind of promotion cell proliferation and differentiation, antidotal material: stem cell secretion factor; 2, the preparation method of stem cell secretion factor is provided; 3, the purposes of stem cell secretion factor is provided.
The present invention is achieved in that
The immortal cell line in stem cell rice source or go down to posterity and the adult stem cell system that builds up through tens of generations to the cultivation in generations up to a hundred, in the stem cells hyperplasia atomization, secreted a large amount of stem cell secretion factors, this is-kind comprised the growth of multiple promotion cell, activation and regenerated materials such as somatomedin, protein, active polypeptide, trace element.Refining stem cell secretion factor through the precision sizing that comprises modern biotechnologies such as separation, purifying is made can promote cell proliferation, differentiation, prevents cell aging.
Stem cell secretion factor is joined the vigor that can strengthen skin cells in the makeup with the ratio of 10~100 μ g/ml, skin to health has anti-ageing, strengthen the function of skin elasticity, skin to damage can automatically nurse, takes good care of and repair, and makes skin tenderer, the more high resilience that becomes; Because stem cell secretion factor contains the various kinds of cell factor, can promote the metabolism of cell simultaneously, be a kind of new cytokine source of keeping ancestral cells propagation and differentiation.Thereby the purified stem cell secretion factor can promote the aging of metabolism, enhancing body immunizing power and the opposing cell of cell, in makeup, healthcare products and regenerative medicine, have splendid application prospect, and will have a tremendous social and economic benefits.
Embodiment
The foundation of embodiment 1, adult stem cell system
At first separate from the adult stem cell in multiple embryonic tissue source, going down to posterity under external serum-free condition and 20~40 be commissioned to train fosterly in purified back, sets up system such as the adult stem cells that comprise hemopoietic stem cell, neural stem cell, liver stem cells, skin progenitor cell, muscle stem cell.Or adopt the genetic modification means, and change over to adult stem cell such as Hoxb-4 gene, telomerase activation subunit, set up the immortal cell line of source of human stem cell.
Cell requires growth conditions good, goes down to posterity once pollutions such as no bacterium, mycoplasma in per 3~4 days; Passage cell needs to form through cytogenetics, clone the detection of experiment, the experiment of nude mice tumorigenesis etc., is confirmed that it is the cell of normal growth state.
The acquisition of embodiment 2, stem cell secretion factor and purifying
In the immortal cell line culturing process of adult stem cell system or source of human stem cell, the propagation of stem cell, atomization can be secreted many somatomedins, protein, active polypeptide, trace element or the like and promote cell growth, activation, regenerated material, by separation, purifying equimolecular biological means, can obtain the purified stem cell secretion factor.
Stem cell secretion factor mainly comprises pHGF (HGF), nerve growth factor (NGF), Urogastron (EGF), fiber mother cell growth factor (FGF), Insulin-Like growth factor (IGF), hemopoietic stem cell stimulating substance etc.In addition, also contain the factor that improves immunizing power in the stem cell secretion factor, as various G CFSs (CSFs), multiple interleukin (IL-1,2,3,4,11, etc.).
Embodiment 3, stem cell secretion factor biological activity determination
Human stem cell excreted factor injection is added 37 ℃ of cultivations in the bone marrow cells in mice by 120 μ g/ holes/sky, the 2nd day meter cell count, and observation of cell metamorphosis under the mirror, compare the propagation that can promote the adherent of cell and stimulation bone marrow cells in mice to the stem cell secretion factor group with control group.
The application in makeup of embodiment 4, stem cell secretion factor
The ratio of stem cell secretion factor with 10~100 μ g/ml joined in the prescription of makeup, make makeup after the homogenization treatment.The makeup that contain stem cell secretion factor have anti-ageing, strengthen the function of skin elasticity, can automatically nurse, take good care of and repair the skin of damage, make skin tenderer, the more high resilience that becomes.
The application in healthcare products of embodiment 5, stem cell secretion factor
Stem cell secretion factor is combined with part food, can be made into healthcare products.That the healthcare products that contain the two have is anti-ageing, enhancing body immunizing power, promote damage or old and feeble tissue regeneration and the effect that improves tissue organ function.
The application in regenerative medicine of embodiment 6, stem cell secretion factor
The ratio of stem cell secretion factor with 10~200 μ g/ml added in the external ancestral cells culture system, bring into play the function that it promotes cell proliferation and differentiation.
Claims (8)
1. stem cell secretion factor and preparation method thereof and application, the immortality cell that it is characterized in that source of human stem cell ties up to excretory in the stem cells hyperplasia atomization, the material that has comprised the growth of multiple promotion cell such as somatomedin, protein, active polypeptide, trace element, activation, regeneration etc., its preparation method is the separation of the immortal cell line → proliferation and differentiation → culture supernatant → various secretory product of source of human stem cell, and its purposes is for making makeup, healthcare products and being applied in the regenerative medicine.
2. the immortal cell line of source of human stem cell according to claim 1, it is characterized in that taking from the adult stem cell that human embryo stem cell and multiple embryonic tissue separation and purification obtain, the different stem cell line that goes down to posterity or set up through the cultivation in tens of generations to generations up to a hundred through genetic modification.
3. adult stem cell according to claim 2 is characterized in that comprising hemopoietic stem cell, neural stem cell, liver stem cells, skin progenitor cell, muscle stem cell or the like at interior tissue stem cell.
4. the immortal cell line of the source of human stem cell through genetic modification according to claim 2 is characterized in that the stem cell line of setting up through comprising the genetic modification of Hoxb-4, telomerase activation subunit etc.
5. the preparation method of stem cell secretion factor according to claim 1 is characterized in that the immortality cell of source of human stem cell is tied up to the various active substances of excretory in the proliferation and differentiation process, and the various means of applied molecular biology get its precision sizing.
6. the cosmetic use of stem cell secretion factor according to claim 1 is characterized in that stem cell secretion factor allocates in the makeup with 1: 50~1: 100 ratio, after the homogenization treatment, makes makeup.
7. the healthcare products purposes of stem cell secretion factor according to claim 1 is characterized in that preparing anti-ageing, enhancing body immunizing power, promotes damage or old and feeble tissue regeneration and the healthcare products that improve tissue organ function.
8. the regenerative medicine purposes of stem cell secretion factor according to claim 1 is characterized in that promoting in the application of stem cells excreted factor factor of cell metabolism and proliferation and differentiation, and is introduced in the application of regenerative medicine.
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CNA200310115014XA CN1618981A (en) | 2003-11-20 | 2003-11-20 | Dry cell secretion factor and its preparation method and application |
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CNA200310115014XA CN1618981A (en) | 2003-11-20 | 2003-11-20 | Dry cell secretion factor and its preparation method and application |
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Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104997708A (en) * | 2015-07-07 | 2015-10-28 | 河南中科干细胞基因工程有限公司 | Obtaining method of stem cell conditioned medium, and application of stem cell conditioned medium in skin ageing resistance |
CN105078777A (en) * | 2014-05-15 | 2015-11-25 | 金凤华 | Mesenchymal stem cell excreted factor essence, and preparation method and application thereof |
CN107982197A (en) * | 2017-11-29 | 2018-05-04 | 中南大学 | Composition and its application containing non-stem cell constituents |
CN108113959A (en) * | 2018-02-12 | 2018-06-05 | 浙江奥瑞健生物技术有限公司 | A kind of stem cell freeze-dried powder for sensitive skin |
CN108125904A (en) * | 2018-02-12 | 2018-06-08 | 浙江奥瑞健生物技术有限公司 | It is a kind of for composition of dryness sensitivity skin and preparation method thereof |
CN108158933A (en) * | 2017-11-29 | 2018-06-15 | 中南大学 | Composition and its application containing non-stem cell constituents |
CN108261389A (en) * | 2018-02-12 | 2018-07-10 | 浙江奥瑞健生物技术有限公司 | A kind of full extracting solution composition of stem cell for being used for sensitive skin and its preparation method and application |
CN108403616A (en) * | 2018-04-28 | 2018-08-17 | 浙江奥瑞健生物技术有限公司 | A kind of radioresistance skin care compositions |
CN108498435A (en) * | 2018-04-28 | 2018-09-07 | 浙江奥瑞健生物技术有限公司 | A kind of emulsion compositions and its application in cosmetics |
CN108498449A (en) * | 2018-04-28 | 2018-09-07 | 浙江奥瑞健生物技术有限公司 | A kind of spray composite and its application in cosmetics |
CN109867718A (en) * | 2019-04-11 | 2019-06-11 | 南京鼓楼医院 | A kind of preparation method and its usage of the composite regenerated factor of high concentration from neural stem cell source |
-
2003
- 2003-11-20 CN CNA200310115014XA patent/CN1618981A/en active Pending
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105078777A (en) * | 2014-05-15 | 2015-11-25 | 金凤华 | Mesenchymal stem cell excreted factor essence, and preparation method and application thereof |
CN104997708A (en) * | 2015-07-07 | 2015-10-28 | 河南中科干细胞基因工程有限公司 | Obtaining method of stem cell conditioned medium, and application of stem cell conditioned medium in skin ageing resistance |
CN107982197A (en) * | 2017-11-29 | 2018-05-04 | 中南大学 | Composition and its application containing non-stem cell constituents |
CN108158933A (en) * | 2017-11-29 | 2018-06-15 | 中南大学 | Composition and its application containing non-stem cell constituents |
CN108113959A (en) * | 2018-02-12 | 2018-06-05 | 浙江奥瑞健生物技术有限公司 | A kind of stem cell freeze-dried powder for sensitive skin |
CN108125904A (en) * | 2018-02-12 | 2018-06-08 | 浙江奥瑞健生物技术有限公司 | It is a kind of for composition of dryness sensitivity skin and preparation method thereof |
CN108261389A (en) * | 2018-02-12 | 2018-07-10 | 浙江奥瑞健生物技术有限公司 | A kind of full extracting solution composition of stem cell for being used for sensitive skin and its preparation method and application |
CN108403616A (en) * | 2018-04-28 | 2018-08-17 | 浙江奥瑞健生物技术有限公司 | A kind of radioresistance skin care compositions |
CN108498435A (en) * | 2018-04-28 | 2018-09-07 | 浙江奥瑞健生物技术有限公司 | A kind of emulsion compositions and its application in cosmetics |
CN108498449A (en) * | 2018-04-28 | 2018-09-07 | 浙江奥瑞健生物技术有限公司 | A kind of spray composite and its application in cosmetics |
CN109867718A (en) * | 2019-04-11 | 2019-06-11 | 南京鼓楼医院 | A kind of preparation method and its usage of the composite regenerated factor of high concentration from neural stem cell source |
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