CN108477395A - A kind of production method of feed additive for ruminant - Google Patents
A kind of production method of feed additive for ruminant Download PDFInfo
- Publication number
- CN108477395A CN108477395A CN201810242621.9A CN201810242621A CN108477395A CN 108477395 A CN108477395 A CN 108477395A CN 201810242621 A CN201810242621 A CN 201810242621A CN 108477395 A CN108477395 A CN 108477395A
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- China
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- added
- filtrate
- bamboo shoot
- ruminant
- feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000282849 Ruminantia Species 0.000 title claims abstract description 16
- 239000003674 animal food additive Substances 0.000 title claims abstract description 11
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 39
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 39
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 39
- 239000011425 bamboo Substances 0.000 claims abstract description 39
- 239000000706 filtrate Substances 0.000 claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 15
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 11
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 11
- 230000004151 fermentation Effects 0.000 claims abstract description 11
- 239000000843 powder Substances 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 8
- 240000008042 Zea mays Species 0.000 claims abstract description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 7
- 235000005822 corn Nutrition 0.000 claims abstract description 7
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 108010059892 Cellulase Proteins 0.000 claims abstract description 4
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims abstract description 4
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 4
- 229940106157 cellulase Drugs 0.000 claims abstract description 4
- 239000012141 concentrate Substances 0.000 claims abstract description 4
- 238000005469 granulation Methods 0.000 claims abstract description 4
- 230000003179 granulation Effects 0.000 claims abstract description 4
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims abstract description 4
- 238000004806 packaging method and process Methods 0.000 claims abstract description 4
- 239000000047 product Substances 0.000 claims abstract description 4
- 241001330002 Bambuseae Species 0.000 claims description 38
- 239000002609 medium Substances 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 239000000654 additive Substances 0.000 claims description 3
- 230000000996 additive effect Effects 0.000 claims description 3
- 229940088598 enzyme Drugs 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 claims description 3
- 238000009630 liquid culture Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims 1
- 235000019629 palatability Nutrition 0.000 abstract description 3
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 244000082204 Phyllostachys viridis Species 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 239000012809 cooling fluid Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 230000003000 nontoxic effect Effects 0.000 abstract 1
- 229930182470 glycoside Natural products 0.000 description 12
- -1 cyanogen glycosides Chemical class 0.000 description 11
- JMANVNJQNLATNU-UHFFFAOYSA-N glycolonitrile Natural products N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 11
- 241001465754 Metazoa Species 0.000 description 8
- 238000009835 boiling Methods 0.000 description 4
- LELOWRISYMNNSU-UHFFFAOYSA-N hydrogen cyanide Chemical compound N#C LELOWRISYMNNSU-UHFFFAOYSA-N 0.000 description 4
- 231100000572 poisoning Toxicity 0.000 description 3
- 230000000607 poisoning effect Effects 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 2
- 235000011949 flavones Nutrition 0.000 description 2
- 150000002213 flavones Chemical class 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 240000006909 Tilia x europaea Species 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Birds (AREA)
- Sustainable Development (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to a kind of production method of feed addictive, especially a kind of production method of feed additive for ruminant.Mix to obtain bamboo shoot shell powder, corn steep liquor and water thoroughly solid medium, what solid medium is sterilized, cooling, aspergillus oryzae ferments one time fermentation feed, the clear water of 2 times of quality is added in one time fermentation feed, it stirs evenly, sterilized, cooling fluid nutrient medium, cellulase and lactobacillus bulgaricus freeze-dried powder are added in liquid medium, it filters to get filtrate after anaerobic fermentation and filter residue, filtrate is concentrated to give concentration filtrate, it will concentrate after filtrate mixes with filter residue again through drying, crushing, granulation, packaging to get finished product.The feed additive for ruminant of the present invention, it is not only non-toxic, good without bitter taste, palatability, moreover it is possible to improve the immunocompetence of ruminant, promote growth.
Description
Technical field
The present invention relates to a kind of production method of feed addictive, especially a kind of making of feed additive for ruminant
Method.
Background technology
Bamboo shoot shell is exactly the shell outside bamboo shoot meat, it is the byproduct after bamboo shoots are processed.Bamboo shoot shell is a still undeveloped important
Resource, currently, the utilization of bamboo shoot shell are still far from perfect, relevant report both domestic and external is all fewer.Present bamboo shoot shell generally uses
Processing mode be that underground is buried or discarded nearby in river shoal, limes marginis, not only cause environmental pollution and a kind of wave of resource
Take, the processing of bamboo shoot shell has become " bottleneck " that puzzlement bamboo shoots process deeply industry is further developed.Bamboo shoot shell is rich in protein, fat
The multiple biological activities such as the multiple nutritional components such as fat, carbohydrate, vitamin, minerals and phytosterol, flavones, phenolic acid
Substance has important physiological activity to animal body, the immunocompetence of animal can be improved, and promotes growth of animal, especially
It is its content of cellulose up to 20% or more, is well suited for feeding ruminants, still, cyanogen glycosides is contained in bamboo shoot shell, cyanogen glycosides not only has hardship
Taste influences the palatability of feed, can also be digested and be released the virose hydrogen cyanide of tool in animal body, hydrogen cyanide is through stomach
After intestinal absorption, animal body can be made to be absorbed in smother, cause the poisoning of animal, if so domestic animal largely searches for food or adopts throughout the year
Bamboo shoot shell is eaten, easily causes poisoning even dead, heavy losses is caused to aquaculture.It, can be by bamboo shoot shell by bamboo shoot shell drying or boiling
In most of cyanogen glycosides destroy, but also part cyanogen glycosides remaining, animal is throughout the year edible to will also result in slow poisoning.So bamboo shoot shell
The quality of detoxification efficiency directly affects its application prospect as feed, the cyanogen glycosides in bamboo shoot shell is only handled containing to safety
Amount standard could preferably apply it to feedstuff industry.
Invention content
The object of the present invention is to provide a kind of using bamboo shoot shell as the production method of the feed additive for ruminant of primary raw material.
The object of the present invention is achieved like this：
Bamboo shoot shell powder will be obtained after bamboo shoot shell drying, crushing, weighs the bamboo shoot shell powder of certain mass, the corn steep liquor of 4-6% is added, water is added to mix thoroughly,
So that material moisture control is obtained solid medium in 55-60%, solid medium is sterilized 30 minutes under the conditions of 121 DEG C, is cooled to
28-32 DEG C, 10% aspergillus oryzae seed liquor is accessed, is mixed thoroughly, in tiling to tray, 3-5 centimetres of thickness, in 28-32 DEG C of temperature, phase
To cultivating 48 hours to obtain one time fermentation feed under conditions of humidity 80-90%.
The clear water of 2 times of quality is added in one time fermentation feed, stirs evenly, sterilizes 15-20 minutes under the conditions of 121 DEG C
Postcooling obtains fluid nutrient medium to 40 DEG C, and cellulase is added in liquid medium, and additive amount is per milliliters of liquid culture medium
30-40 unit fiber element enzymes are added, then 0.01% lactobacillus bulgaricus freeze-dried powder is added in liquid medium, in 36-40
It filters to get filtrate after anaerobic fermentation 35-40 hours under the conditions of DEG C and filter residue, filtrate, which is concentrated to 20-25 ° of Bx, must concentrate filtrate, will
Concentration filtrate mixes with filter residue, then through drying, crushing, granulation, packaging to get finished product.
The invention also includes such features：The production method of the aspergillus oryzae seed liquor is：In 5 ° of B é brewer's worts
1% corn steep liquor is added, sterilizes 20 minutes at 121 DEG C after stirring evenly, is cooled to 28-32 DEG C, aspergillus oryzae is inoculated with, in 28-32
DEG C, cultivate 25-30 hours under conditions of rotary shaker 160-200rpm.
Measure respectively untreated bamboo shoot shell, be dried to 8% bamboo shoot shell below of water content, the bamboo shoot shell of 100 DEG C of boilings 30 minutes and this
The content of cyanogen glycosides, as a result see the table below in invention feed addictive：
| Serial number | Title | Cyanogen glycosides content（In terms of over dry）mg/kg |
| 1 | Untreated bamboo shoot shell | 122.93 |
| 2 | Dry bamboo shoot shell | 54.45 |
| 3 | Boiling bamboo shoot shell | 32.27 |
| 4 | Feed addictive of the present invention | 2.25 |
As can be seen from the above table, bamboo shoot shell is unprocessed, and cyanogen glycosides content is very high, up to 122.93 mg/kg, and pass through drying or
The bamboo shoot shell of person's boiling, cyanogen glycosides content is substantially reduced, but still has higher residual, is carried out to bamboo shoot shell using the method for the present invention
After processing, 98% or more cyanogen glycosides is all removed, so being safe using the feed addictive feeding ruminants of the present invention.
The beneficial effects of the invention are as follows：
First, feed additive for ruminant of the invention have passed through fermentation in the production process, improve yellow in bamboo shoot shell
The immunocompetence of ruminant can be improved in the physiological activity of ketone, flavones, promotes growth.
Second, feed additive for ruminant of the invention have passed through fermentation, not only eliminates in the production process
The bitter taste of cyanogen glycosides also has the pico- tart flavour of fermentation, improves palatability.
The amount of third, feed additive for ruminant of the invention institute cyanogentic glycoside is extremely low, and it is extremely to pacify that ruminant is edible
Complete.
4th, feed additive for ruminant of the invention is main former using the waste bamboo shoot shell of bamboo shoots deep processing enterprise
Material, not only turns waste into wealth, and solves the problems, such as that bamboo shoot shell pollutes environment, can also save food, be to achieve many things at one stroke.
Specific implementation mode
The strain Aspergillus oryzae CICC40636 that the present invention uses is purchased in Chinese industrial Microbiological Culture Collection administrative center.
1% corn steep liquor is added in 5 ° of B é brewer's worts, sterilizes 20 minutes at 121 DEG C after stirring evenly, is cooled to 30
DEG C, it is inoculated with aspergillus oryzae, aspergillus oryzae seed liquor is cultivated 28 hours to obtain under conditions of 30 DEG C, rotary shaker 180rpm.
Bamboo shoot shell powder will be obtained after bamboo shoot shell drying, crushing, weighs the bamboo shoot shell powder of certain mass, 5% corn steep liquor is added, water is added to mix
It is even, so that material moisture control is obtained solid medium 58%, solid medium is sterilized 30 minutes under the conditions of 121 DEG C, is cooled to
30 DEG C, 10% aspergillus oryzae seed liquor is accessed, is mixed thoroughly, in tiling to tray, 3-5 centimetres of thickness, in 30 DEG C of temperature, relative humidity
One time fermentation feed is cultivated 48 hours to obtain under conditions of 85%.
The clear water of 2 times of quality is added in one time fermentation feed, stirs evenly, sterilizes 15-20 minutes under the conditions of 121 DEG C
Postcooling obtains fluid nutrient medium to 40 DEG C, and cellulase is added in liquid medium, and additive amount is per milliliters of liquid culture medium
35 unit fiber element enzymes are added, then 0.01% lactobacillus bulgaricus freeze-dried powder is added in liquid medium, in 37 DEG C of conditions
Lower anaerobic fermentation filters to get filtrate after 37 hours and filter residue, and filtrate, which is concentrated to 24 ° of Bx, must concentrate filtrate, by concentration filtrate and filter
Slag mixes, then through drying, crushing, granulation, packaging to get finished product.
Claims (1)
1. a kind of production method of feed additive for ruminant, it is characterized in that being prepared by following process：
(1) 1% corn steep liquor is added in 5 ° of B é brewer's worts, sterilizes 20 minutes at 121 DEG C after stirring evenly, is cooled to 28-32
DEG C, it is inoculated with aspergillus oryzae, aspergillus oryzae seed is cultivated 25-30 hours to obtain under conditions of 28-32 DEG C, rotary shaker 160-200rpm
Liquid；
(2) bamboo shoot shell powder will be obtained after bamboo shoot shell drying, crushing, weigh the bamboo shoot shell powder of certain mass, the corn steep liquor of 4-6% is added, water is added to mix
It is even, so that material moisture control is obtained solid medium in 55-60%, solid medium is sterilized 30 minutes under the conditions of 121 DEG C, it is cold
But to 28-32 DEG C, 10% aspergillus oryzae seed liquor is accessed, is mixed thoroughly, in tiling to tray, 3-5 centimetres of thickness, in temperature 28-32
DEG C, one time fermentation feed is cultivated 48 hours to obtain under conditions of relative humidity 80-90%；
(3) the clear water of 2 times of quality is added in one time fermentation feed, stirs evenly, after sterilizing 15-20 minutes under the conditions of 121 DEG C
It is cooled to 40 DEG C and obtains fluid nutrient medium, cellulase is added in liquid medium, additive amount is to add per milliliters of liquid culture medium
Enter 30-40 unit fiber element enzymes, then 0.01% lactobacillus bulgaricus freeze-dried powder is added in liquid medium, at 36-40 DEG C
Under the conditions of anaerobic fermentation filter to get filtrate after 35-40 hours and filter residue, filtrate, which is concentrated to 20-25 ° of Bx, must concentrate filtrate, will be dense
Contracting filtrate mixes with filter residue, then through drying, crushing, granulation, packaging to get finished product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810242621.9A CN108477395A (en) | 2018-03-23 | 2018-03-23 | A kind of production method of feed additive for ruminant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810242621.9A CN108477395A (en) | 2018-03-23 | 2018-03-23 | A kind of production method of feed additive for ruminant |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108477395A true CN108477395A (en) | 2018-09-04 |
Family
ID=63319429
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810242621.9A Withdrawn CN108477395A (en) | 2018-03-23 | 2018-03-23 | A kind of production method of feed additive for ruminant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108477395A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116391799A (en) * | 2023-03-06 | 2023-07-07 | 广东省农业科学院蚕业与农产品加工研究所 | Feed additive based on bamboo shoot byproducts and preparation process thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3691808B2 (en) * | 2002-05-21 | 2005-09-07 | 独立行政法人農業・生物系特定産業技術研究機構 | Silage preparation method using filamentous fungi |
| CN103609852A (en) * | 2013-12-03 | 2014-03-05 | 五峰千丈白毫茶业有限公司 | Tea feeds and preparation method |
| CN104996722A (en) * | 2015-08-13 | 2015-10-28 | 江南大学 | Method of two-step united multi-strain fermented feed |
-
2018
- 2018-03-23 CN CN201810242621.9A patent/CN108477395A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3691808B2 (en) * | 2002-05-21 | 2005-09-07 | 独立行政法人農業・生物系特定産業技術研究機構 | Silage preparation method using filamentous fungi |
| CN103609852A (en) * | 2013-12-03 | 2014-03-05 | 五峰千丈白毫茶业有限公司 | Tea feeds and preparation method |
| CN104996722A (en) * | 2015-08-13 | 2015-10-28 | 江南大学 | Method of two-step united multi-strain fermented feed |
Non-Patent Citations (3)
| Title |
|---|
| 余斌: "笋壳废弃物发酵料在肉羊饲喂中的应用", 《中国畜牧业》 * |
| 曾俊棋: "笋壳饲料的资源化利用及其氰甙脱毒方法的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 赵丽萍等: "笋壳作为动物饲料利用研究进展", 《中国畜牧杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116391799A (en) * | 2023-03-06 | 2023-07-07 | 广东省农业科学院蚕业与农产品加工研究所 | Feed additive based on bamboo shoot byproducts and preparation process thereof |
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Application publication date: 20180904 |
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