CN108476971B - A kind of propagation method of ornamental type cotton - Google Patents
A kind of propagation method of ornamental type cotton Download PDFInfo
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- CN108476971B CN108476971B CN201810260526.1A CN201810260526A CN108476971B CN 108476971 B CN108476971 B CN 108476971B CN 201810260526 A CN201810260526 A CN 201810260526A CN 108476971 B CN108476971 B CN 108476971B
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- cotton
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- ornamental type
- type cotton
- nakamise
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The present invention relates to ornamental type plant cultivation field, in particular to a kind of propagation method of ornamental type cotton, comprising the following steps: using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 harvests F1 generation seed as male parent, hybridization；It is selfed after the plantation of F1 generation seed, harvests F2 for seed；F2 is planted for seed or its progeny seed, and screening obtains the ornamental type cotton of target flower shape；The ornamental type cotton screened is grafted as scion, and culture obtains the young plant of ornamental type cotton.Inventors have surprisingly discovered that, using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 is as male parent, hybridization, the seed selfing of harvest, then further plantation, it can obtain windmill-shape or rose or its approximate flower-shape ornamental type cotton, the ornamental type cotton by the way of grafting in high volume can quickly produce ornamental type cotton cotton plant, meet cotton and watch the market demand.
Description
Technical field
The present invention relates to ornamental type plant cultivation fields, in particular to a kind of propagation method of ornamental type cotton.
Background technique
In Plant Taxonomy, cotton belongs to plant kingdom (Plantae), Angiospermae (Angiospermae), Shuangzi
Leaf plant guiding principle (Dicotyledoneae), Malvales (Malvales), Malvaceae (Malvaceae), Gossypium (Gossypium).
Cotton in China plantation is with a long history, and cotton harvests natural fiber as one of major economic crops, is used for textile industry.
In recent years, with the improvement of living standards, people are higher and higher to the pursuit of beauty, start to plant at home a large amount of ornamental
Type plant, cultivates one's taste, and improves living environment.Cotton have indeterminate growth habit, environmental condition be suitable for, can with perennation,
And florescence is long, and in addition Post flowering blow-of-cottons cotton boll is also considered as a kind of flower, so cotton is also planted initially as ornamental type
Species are planted.
Since the improvement of cotton flower shape is relatively difficult, vulnerable to the influence of illumination and temperature, so cotton flower shape
The fixation of variation type is extremely difficult, and rose or windmill-shape flower cotton variety at least needed for 7 generations could cultivate success, breeding
Time is long.Moreover, since rose or windmill-shape flower cotton variety heredity is stable, can be selfed in extension process
It reserves seed for planting, it is difficult to protect intellectual property.
In view of this, the present invention is specifically proposed.
Summary of the invention
The purpose of the present invention is to provide a kind of propagation method of ornamental type cotton, this method be by cleisogamy material and
Island cotton variety Xinhai 21 is hybridized, and then screening obtains windmill-shape or rose ornamental type cotton after being selfed, and is obtained
To ornamental type cotton bred by the way of grafting, shorten cultivation age limit, and be quickly obtained ornamental type convenient for stablizing batch
Cotton seedling.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of propagation method of ornamental type cotton, comprising the following steps:
Using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 hybridizes as male parent, harvest
F1 generation seed；
It is selfed after the F1 generation seed plantation, harvests F2 for seed；
F2 is planted for seed or its progeny seed, and screening obtains the ornamental type cotton of target flower shape；
The ornamental type cotton screened is grafted as scion, and culture obtains the young plant of ornamental type cotton.
Inventors have surprisingly discovered that using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 is made
For male parent, hybridization, the seed selfing of harvest, then further plantation, can obtain windmill-shape or rose or its approximate flower-shape
Ornamental type cotton meets cotton and watches the market demand.Further, the ornamental type cotton by the way of grafting, can be big
Batch quickly produces ornamental type cotton cotton plant, meets the market demand.
Further, the grafting connects for bud.Bud connect it is easily operated, be convenient for mass propagation.
Further, the bud selecting cotton stock for growing to two panels true leaf, with the tender of the ornamental type cotton
Bud is grafted as scion.
Specifically, the step of bud connects specifically:
Cotton seedling top is cut off in the cotyledon upper level of nakamise 24, then 1~2cm of vertical cut vertically downward in the middle part of stalk
Notch；
The tender shoots of ornamental type cotton removes extra blade and stalk, retains 1 true leaf and the stem of 3~5cm, in the stem
A knife wedgewise is sideling cut respectively in bottom both sides；
The tender shoots cut is injected into stock notch, binding is placed on and continues to cultivate in greenhouse.
By the operation, obtained grafting success rate is high, is 100%.
Further, the stem of the tender shoots of the ornamental type cotton is whittled into wedge shape using the cutter after disinfection.Generally use wine
Essence carries out cutting movement to cutter such as scalpel.
The tender shoots cut is injected into stock notch, pays attention to being inserted to bottom, interface is wound repeatedly with cord, is tightened.It puts
It sets and continues to cultivate in the greenhouse, the cotton seedling survived after two weeks can list marketing.
Further, the length of the stem of wedge-shaped tender shoots is 1~2cm.
In order to screen to obtain the ornamental type cotton of target flower shape, further, the male parent and maternal plant
Number is not less than 10 plants, and the strain number of F1 generation seed plantation is not less than 100 plants.
Further, progeny seed is F3 for above seed, preferably F3-F5 generation in generation.Certainly, most preferably F2 generation kind
Son plantation.The ornamental type cotton for screening obtained target flower shape in this way is grafted as scion, eliminates screening and stablizes
The step of kind, simplifies step, saves the time, and be easy to grasp the control of cotton seed.
The control for grasping cotton seed refers to: since the cotton material of batch breeding can be selfed in selfing process, from
Hand over offspring that trait segregation can occur, it cannot be guaranteed that there is target flower-shape in obtained offspring, it can only be by continuing to buy cotton Miao Caineng
Rose or windmill-shape flower cotton is obtained, intellectual property can be protected.
Further, F3 is to plant F2 for seed for seed, and the seed of target flower shape cotton plant is collected in plant division, is obtained
F3 is for seed.
F2 during the growth process, in florescence, selects the single plant of satisfactory flower shape for cotton plant, and F2 generation
The achievable selfing of single plant.
Preferably, F2 is not less than 500 plants for the scale that seed is planted.
Further, the F3 that plant division is collected is planted for seed point group, and the F3 group for selecting single plant is target flower-shape entirely
The cotton plant of shape harvests seed, obtains F4 for seed.
The F3 that different cotton plants is collected is for seed in different ecological region plantings, and Ji Fen group plants, and different groups realizes
The trait segregation phenomenon or flower shape of existing flower shape unsightly, eliminate these groups, the F3 group for selecting single plant is entirely
The cotton plant of ornamental type cotton harvests seed, obtains F4 for seed.
Preferably, the F3 of every plant of collection is not less than 100 plants for the scale that seed is planted.
F4 generation and F5 are planted for seed, are to screen to obtain corresponding plant type.
Further, the F4 is planted for seed, and selection target flower shape and short bar and compact plant single plant mix
Harvest, obtains F5 for seed.
Further, the F5 is planted for seed, and selection target flower shape and short bar and compact plant single plant are distinguished
Harvest, obtains F6 for seed.
Preferably, the scale that F4 generation and/or F5 plants for seed is not less than 500 plants.
F6 is in order to which corresponding character is further purified for seed plantation.
Further, the F6 that plant division is collected is planted for seed point group, selects the F6 group of single plant generally short bar and plant type
The cotton plant of compact target flower shape, and poor plant type single plant is eliminated, mixing harvest seed obtains F7 for seed；
The F6 of every plant of collection is not less than 100 plants for the scale that seed is planted.
Further, the F7 is planted for seed, obtains flower shape and the stable ornamental type cotton of plant type.
That is F7 has stable character inheritance for seed and its later seed.
It should be noted that since the character of the target flower shape of ornamental type cotton provided by the invention is recessiveness
Shape, flower is wide-open after the plantation of F1 generation seed, self cannot hybridize, therefore, is selfed, obtained using human assistance
F2 for the flower shape of trait segregation can be obtained after seed plantation.Because target flower shape of the invention is or approximate
For windmill-shape or rosette, the flower of both flower-shapes is all endless Full-open, therefore, just can no longer be needed with self hybridization
Want extraneous auxiliary selfing.That is, the ornamental type cotton that the present invention screens completes selfing during plantation.
In the present invention, grafting stock used can be selected from any one cotton seed, be not particularly limited to the source of stock,
Such as graft stock used and be selected from any one of following cotton seed: nakamise 24, nakamise 36, nakamise 45, nakamise 49, in
Cotton 53, nakamise 55, nakamise 62, nakamise 63, nakamise 65, nakamise 66, nakamise 71, nakamise 87, TM-1.
Further, the target flower shape is windmill-shape or rose.
Compared with prior art, the invention has the benefit that
(1) inventors have surprisingly discovered that, using cleisogamy material C J1548143 as female parent, the new sea 21 of island cotton variety
Number be used as male parent, hybridization, harvest seed selfing, then further plantation, windmill-shape or rose or its approximate flower can be obtained
The ornamental type cotton of shape, energy rapid batch produces ornamental type cotton plant by the way of grafting, meets cotton and watches the market demand.
(2) propagation method of ornamental type cotton provided by the invention, the mode for selecting specific bud to connect are grafted, are easy to
Mass propagation is convenient in operation.
(3) propagation method of ornamental type cotton provided by the invention grafts the ornamental type cotton of target flower shape used
The step of spending the tender shoots that the cotton seed plantation of low generation can be selected, eliminating screening distinct variety, simplifies step, saves the time,
And it is easy to grasp the control of cotton seed.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described.
Fig. 1 be the present embodiments relate to cleisogamy material C J1548143 flower photo；
Fig. 2 be the present embodiments relate to Xinhai 21 flower photo；
Fig. 3 is the flower photo for the windmill-shape flower cotton that the embodiment of the present invention is cultivated；
Fig. 4 is the flower photo for the rose flower cotton that the embodiment of the present invention is cultivated；
Fig. 5 is the growth conditions photo that stock used is grafted in the embodiment of the present invention；
Fig. 6 is the tender shoots photo of windmill-shape or rose flower cotton plant that the embodiment of the present invention is won；
Fig. 7 is the cotton seedling photo after grafting of the embodiment of the present invention；
Fig. 8 is the cotton seedling photo cultivated after the embodiment of the present invention is grafted two weeks.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Embodiment 1
The propagation method of windmill-shape flower cotton
1. cleisogamy material C J1548143 and island cotton variety Xinhai 21 configure cross combination
Cleisogamy material C J1548143: on the day of florescence flower blooms loose powder pollination, petal is closed, and does not open, complete
It pollinates at self, flower is as shown in Figure 1.The material can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute or commercially available purchase obtains
.
Xinhai 21: island cotton variety, flower are as shown in Figure 2.The material can be studied from Chinese Academy of Agricultural Sciences cotton
It is obtained.Precocity, high yield, high-quality long-staple cotton new varieties Xinhai 21 [J] Cotton, 2004,31 (1): 31, this article has
Disclose the material.
Chamber planting cleisogamy material C J1548143 and each 10 plants of Xinhai 21 in Hainan, with cleisogamy material
CJ1548143 is pollinated, cotton boll maturation harvests F1 seed as maternal, florescence emasculation with the pollen of Xinhai 21.In the greenhouse
100 plants of F1 plant are planted, are selfed, F2 seed is harvested.
2. selecting windmill-shape flower single plant
500 plants of F2 single plant is planted in the greenhouse, and continuous 10 day morning 8:00-12:00 in florescence selects windmill-shape flower
Cotton plant finally selects 5 plants respectively, and windmill-shape flower is as shown in Figure 3.
3. stock
Nakamise 24: Upland Cotton can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute.In short season cotton new varieties-
24 [J] Cottons of cotton, 1995, (11): 31.
It is planted in nutritive cube, it is spare to grow to two panels true leaf in the greenhouse, sees Fig. 5.
4. the propagation by grafiting of windmill-shape flower cotton plant
The tender shoots (see Fig. 6) for the windmill-shape flower cotton plant chosen is won with scissors, bottom is placed in clear water, spare.With
Scalpel after alcohol disinfecting is close to cotyledon upper level in stock and cuts off cotton seedling top, then straight vertically downward in the middle part of stalk
Cut the notch of 1~2cm.The tender shoots for taking out ready windmill-shape flower cotton plant, removes extra blade and stalk, only retains 1
The stem of true leaf and 3~5cm, the scalpel after disinfecting in alcohol sideling cut a knife wedgewise (1~2cm) on bottom both sides respectively.
Then, the tender shoots cut is injected into stock notch, pays attention to being inserted to bottom, interface is wound repeatedly with cord, is tightened (see figure
7).It is placed on and continues to cultivate in greenhouse, the cotton seedling survived after two weeks can list marketing (see Fig. 8).
Embodiment 2
The propagation method of rose flower cotton
1. cleisogamy material C J1548143 and island cotton variety Xinhai 21 configure cross combination
Cleisogamy material C J1548143: on the day of florescence flower blooms loose powder pollination, petal is closed, and does not open, complete
It pollinates at self, flower is as shown in Figure 1.The material can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute or commercially available purchase obtains
.
Xinhai 21: island cotton variety, flower are as shown in Figure 2.The material can be studied from Chinese Academy of Agricultural Sciences cotton
It is obtained.Precocity, high yield, high-quality long-staple cotton new varieties Xinhai 21 [J] Cotton, 2004,31 (1): 31, this article has
Disclose the material.
Chamber planting cleisogamy material C J1548143 and each 10 plants of Xinhai 21 in Hainan, with cleisogamy material
CJ1548143 is pollinated, cotton boll maturation harvests F1 seed as maternal, florescence emasculation with the pollen of Xinhai 21.In the greenhouse
100 plants of F1 plant are planted, are selfed, F2 seed is harvested.
2. selecting rose flower single plant
500 plants of F2 single plant is planted in the greenhouse, and continuous 10 day morning 8:00-12:00 in florescence selects rose flower
Cotton plant finally selects 5 plants respectively, and rose flower is as indicated at 4.
3. stock
Nakamise 24: Upland Cotton can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute.In short season cotton new varieties-
24 [J] Cottons of cotton, 1995, (11): 31.
It is planted in nutritive cube, it is spare to grow to two panels true leaf in the greenhouse, sees Fig. 5.
4. the propagation by grafiting of rose flower cotton plant
The tender shoots (see Fig. 6) for the rose flower cotton plant chosen is won with scissors, bottom is placed in clear water, spare.With
Scalpel after alcohol disinfecting is close to cotyledon upper level in stock and cuts off cotton seedling top, then straight vertically downward in the middle part of stalk
Cut the notch of 1~2cm.The tender shoots for taking out ready rose flower cotton plant, removes extra blade and stalk, only retains 1
The stem of true leaf and 3~5cm, the scalpel after disinfecting in alcohol sideling cut a knife wedgewise (1~2cm) on bottom both sides respectively.
Then, the tender shoots cut is injected into stock notch, pays attention to being inserted to bottom, interface is wound repeatedly with cord, is tightened (see figure
7).It is placed on and continues to cultivate in greenhouse, the cotton seedling survived after two weeks can list marketing (see Fig. 8).
Embodiment 3
The propagation method of the ornamental type cotton of windmill-shape or rose flower
1. cleisogamy material C J1548143 and island cotton variety Xinhai 21 configure cross combination
Cleisogamy material C J1548143: on the day of florescence flower blooms loose powder pollination, petal is closed, and does not open, complete
It pollinates at self, flower is as shown in Figure 1.The material can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute or commercially available purchase obtains
.
Xinhai 21: island cotton variety, flower are as shown in Figure 2.The material can be studied from Chinese Academy of Agricultural Sciences cotton
It is obtained.Precocity, high yield, high-quality long-staple cotton new varieties Xinhai 21 [J] Cotton, 2004,31 (1): 31, this article has
Disclose the material.
Chamber planting cleisogamy material C J1548143 and each 20 plants of Xinhai 21 in Hainan, with cleisogamy material
CJ1548143 is pollinated, cotton boll maturation harvests F1 seed as maternal, florescence emasculation with the pollen of Xinhai 21.In the greenhouse
200 plants of F1 plant are planted, are selfed, F2 seed is harvested.
2. selection target flower-shape flower single plant
1000 plants of F2 single plant, florescence continuous 10 day morning 8:00-12:00 selection windmill-shape or rose are planted in the greenhouse
Rare shape flower single plant, final choice go out 20 plants, harvest F3 seed.
Continue to plant F3 seed in the greenhouse, continuous 10 day morning 8:00-12:00 in florescence selects windmill-shape or rose
Flower single plant；
It is grafted using the method in embodiment 1 or embodiment 2, the cotton seedling largely grafted can be used to commercially available.
Same method harvests the seed, that is, F4 seed with windmill-shape or rose flower character after the plantation of F3 seed.
Continue to plant F4 seed, florescence continuous 10 day morning 8:00-12:00 selection windmill-shape or rose flower list in the greenhouse
Strain；It is grafted using the method in embodiment 1 or embodiment 2, the cotton seedling largely grafted can be used to commercially available.
And so on, the windmill-shape or rose flower single plant that can will be screened after F5 seed, F6 seed, the plantation of F7 seed,
It is grafted using the method in embodiment 1 or embodiment 2, the cotton seedling largely grafted can be used to commercially available.
It should be noted that embodiment 1 and embodiment 2 can carry out simultaneously, it can also separate and carry out, it is general to be simultaneously
It can.
Multiple a large amount of contents for repeating to implement embodiment 1-3, energy stable screening obtain ornamental type windmill-shape flower cotton
Windmill cotton and the rose flower cotton rose cotton of ornamental type, illustrate that a kind of screening technique of ornamental type cotton provided by the invention is steady
It is fixed effective.
Obtained ornamental type cotton ornamental type cotton by the way of grafting is screened, high-volume quickly produces ornamental type
Cotton cotton plant meets cotton and watches the market demand.
In summary, the propagation method of a kind of ornamental type cotton provided by the invention, with cleisogamy material C J1548143
As female parent, island cotton variety Xinhai 21 is as male parent, and hybridization, the seed selfing of harvest, then further plantation, obtains wind
Vehicle shape or rose or its approximate flower-shape ornamental type cotton, energy rapid batch produces ornamental type cotton plant by the way of grafting,
Graft survival rate 100% meets cotton and watches the market demand.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from of the invention
Many other change and modification can be made in the case where spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (9)
1. a kind of propagation method of ornamental type cotton, which comprises the following steps:
Using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 harvests F1 generation as male parent, hybridization
Seed；
It is selfed after the F1 generation seed plantation, harvests F2 for seed；
F2 is planted for seed or its progeny seed；
The ornamental type cotton screened is grafted as scion, and culture obtains the young plant of ornamental type cotton；
The ornamental type cotton flower shape is windmill-shape or rose.
2. the propagation method of ornamental type cotton according to claim 1, which is characterized in that the grafting connects for bud.
3. the propagation method of ornamental type cotton according to claim 2, which is characterized in that the bud selecting is with growing to two
The cotton stock of piece true leaf is grafted using the tender shoots of the ornamental type cotton as scion.
4. the propagation method of ornamental type cotton according to claim 2, which is characterized in that the step of bud connects is specific
Are as follows:
Cotton seedling top is cut off in the cotyledon upper level of nakamise 24, then 1~2cm of vertical cut is cut vertically downward in the middle part of stalk
Mouthful；
The tender shoots of ornamental type cotton removes extra blade and stalk, retains 1 true leaf and the stem of 3~5cm, in the bottom of the stem
A knife wedgewise is sideling cut respectively in both sides；
The tender shoots cut is injected into stock notch, binding is placed on and continues to cultivate in greenhouse.
5. the propagation method of ornamental type cotton according to claim 4, which is characterized in that the tender shoots of the ornamental type cotton
Stem using disinfection after cutter be whittled into wedge shape.
6. the propagation method of ornamental type cotton according to claim 5, which is characterized in that the length of the stem of wedge-shaped tender shoots
For 1~2cm.
7. the propagation method of ornamental type cotton according to claim 1, which is characterized in that the male parent and maternal plantation
Strain number is not less than 10 plants, and the strain number of F1 generation seed plantation is not less than 100 plants.
8. the propagation method of ornamental type cotton according to claim 1, which is characterized in that the progeny seed be F3 generation with
On seed.
9. the propagation method of ornamental type cotton according to claim 1-8, which is characterized in that used in the grafting
Stock be selected from any one of following cotton seed: nakamise 24, nakamise 36, nakamise 45, nakamise 49, nakamise 53, in
Cotton 55, nakamise 62, nakamise 63, nakamise 65, nakamise 66, nakamise 71, nakamise 87, TM-1.
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| CN112400645B (en) * | 2020-11-16 | 2024-03-29 | 安徽省农业科学院棉花研究所 | Cultivation method of high-temperature-resistant cotton new variety |
| CN113287514B (en) * | 2021-07-07 | 2022-11-29 | 安徽省农业科学院棉花研究所 | Propagation method of mottled leaf ornamental cotton |
| CN113692933B (en) * | 2021-09-24 | 2022-09-16 | 湖北省农业科学院经济作物研究所 | Method for ornamental cultivation by using cotton |
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| US20170367246P1 (en) * | 2016-06-21 | 2017-12-21 | Walters Gardens, Inc. | Monarda plant named 'cotton candy' |
| CN106718769B (en) * | 2017-01-19 | 2020-04-03 | 中国农业科学院棉花研究所 | Method for identifying temperature-sensitive characteristic of cotton closed pollination material |
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