CN108476971A - A kind of propagation method of ornamental type cotton - Google Patents

A kind of propagation method of ornamental type cotton Download PDF

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Publication number
CN108476971A
CN108476971A CN201810260526.1A CN201810260526A CN108476971A CN 108476971 A CN108476971 A CN 108476971A CN 201810260526 A CN201810260526 A CN 201810260526A CN 108476971 A CN108476971 A CN 108476971A
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cotton
ornamental type
seed
nakamise
type cotton
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CN108476971B (en
Inventor
李威
刘记
裴小雨
刘艳改
杨代刚
周晓箭
马雄风
贺昆仑
张飞
任中英
孙宽
宋成祥
张文生
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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Institute of Cotton Research of Chinese Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The present invention relates to ornamental type plant cultivation field, more particularly to a kind of propagation method of ornamental type cotton includes the following steps:Using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 harvests F1 generation seed as male parent, hybridization;It is selfed after the plantation of F1 generation seed, F2 is for seed for harvest;F2 is planted for seed or its progeny seed, and screening obtains the ornamental type cotton of target flower shape;The ornamental type cotton screened is grafted as scion, and culture obtains the young plant of ornamental type cotton.Inventors have surprisingly discovered that, using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 is as male parent, hybridization, the seed selfing of harvest, then further plantation, it can obtain windmill-shape or rose or its approximate flower-shape ornamental type cotton, the ornamental type cotton by the way of grafting in high volume can quickly produce ornamental type cotton cotton plant, meet cotton and watch the market demand.

Description

A kind of propagation method of ornamental type cotton
Technical field
The present invention relates to ornamental type plant cultivation fields, in particular to a kind of propagation method of ornamental type cotton.
Background technology
In Plant Taxonomy, cotton belongs to plant kingdom (Plantae), Angiospermae (Angiospermae), Shuangzi Leaf plant guiding principle (Dicotyledoneae), Malvales (Malvales), Malvaceae (Malvaceae), Gossypium (Gossypium). Cotton in China plantation is with a long history, and cotton harvests natural fiber as one of major economic crops, is used for textile industry.
In recent years, as living standard improves, people are higher and higher to beautiful pursuit, start to plant at home a large amount of ornamental Type plant, cultivates one's taste, and improves living environment.Cotton have indeterminate growth habit, environmental condition is suitable, can with perennation, And florescence is long, and in addition Post flowering blow-of-cottons cotton boll is also considered as a kind of flower, so cotton also begins to plant as ornamental type Species are planted.
Since the improvement of cotton flower shape is relatively difficult, it is easily illuminated by the light the influence with temperature, so cotton flower shape The fixation of variation type is extremely difficult, and rose or windmill-shape flower cotton variety at least needed for 7 generations could cultivate success, breeds Time is long.Moreover, in extension process, since rose or windmill-shape flower cotton variety heredity is stable, can be selfed It reserves seed for planting, it is difficult to protect intellectual property.
In view of this, special propose the present invention.
Invention content
The purpose of the present invention is to provide a kind of propagation method of ornamental type cotton, this method be by cleisogamy material and Island cotton variety Xinhai 21 is hybridized, and then screening obtains windmill-shape or rose ornamental type cotton after selfing, obtains To ornamental type cotton using grafting by the way of breed, shorten cultivation age limit, and convenient for stablize batch be quickly obtained ornamental type Cotton seedling.
In order to realize that the above-mentioned purpose of the present invention, spy use following technical scheme:
A kind of propagation method of ornamental type cotton, includes the following steps:
Using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 hybridizes as male parent, harvest F1 generation seed;
It is selfed after the F1 generation seed plantation, F2 is for seed for harvest;
F2 is planted for seed or its progeny seed, and screening obtains the ornamental type cotton of target flower shape;
The ornamental type cotton screened is grafted as scion, and culture obtains the young plant of ornamental type cotton.
Inventors have surprisingly discovered that using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 is made For male parent, hybridization, the seed selfing of harvest, then further plantation, can obtain windmill-shape or rose or its approximate flower-shape Ornamental type cotton meets cotton and watches the market demand.Further, the ornamental type cotton by the way of grafting, can be big Batch quickly produces ornamental type cotton cotton plant, meets the market demand.
Further, the grafting connects for bud.Bud connect it is easily operated, be convenient for mass propagation.
Further, the bud selecting cotton stock for growing to two panels true leaf, with the tender of the ornamental type cotton Bud is grafted as scion.
Specifically, the step of bud connects be specially:
On the cotyledon upper level excision cotton seedling top of nakamise 24, then 1~2cm of vertical cut vertically downward in the middle part of stalk Notch;
The tender shoots of ornamental type cotton removes extra blade and stalk, retains 1 true leaf and the stem of 3~5cm, in the stem A knife wedgewise is sideling cut respectively in bottom both sides;
The tender shoots cut is injected into stock notch, binding is placed in greenhouse and continues to cultivate.
By the operation, obtained grafting success rate is high, is 100%.
Further, the stem of the tender shoots of the ornamental type cotton is whittled into wedge shape using the cutter after disinfection.Generally use wine Essence carries out cutting action to cutter such as scalpel.
The tender shoots cut is injected into stock notch, pays attention to being inserted to bottom, interface is wound repeatedly with cord, is tightened.It puts It sets and continues to cultivate in the greenhouse, the cotton seedling survived after two weeks can list marketing.
Further, the length of the stem of wedge-shaped tender shoots is 1~2cm.
In order to screen to obtain the ornamental type cotton of target flower shape, further, the male parent and maternal plant Number is not less than 10 plants, and the strain number of F1 generation seed plantation is not less than 100 plants.
Further, progeny seed is F3 for above seed, preferably F3-F5 generations in generation.Certainly, most preferably F2 generation kinds Son plantation.The ornamental type cotton for the target flower shape that screening obtains in this way is grafted as scion, eliminates screening and stablizes The step of kind, simplifies step, saves the time, and is easy to grasp the control of cotton seed.
Grasp cotton seed control refer to:Since the cotton material of batch breeding can be selfed in selfing process, from Hand over offspring that trait segregation can occur, it cannot be guaranteed that there is target flower-shape in obtained offspring, it can only be by continuing to buy cotton seedling ability Rose or windmill-shape flower cotton is obtained, intellectual property can be protected.
Further, F3 is to plant F2 for seed for seed, and the seed of target flower shape cotton plant is collected in plant division, is obtained F3 is for seed.
F2 in growth course, in florescence, selects the single plant of satisfactory flower shape for cotton plant, and F2 generations Single plant can be completed to be selfed.
Preferably, F2 is not less than 500 plants for the scale that seed is planted.
Further, the F3 that plant division is collected is planted for seed point group, and the F3 groups for selecting single plant are target flower-shape entirely The cotton plant of shape harvests seed, obtains F4 for seed.
The F3 that different cotton plants is collected is for seed in different ecological region plantings, and Ji Fen groups plant, and different groups realizes The trait segregation phenomenon or flower shape of existing flower shape unsightly, eliminate these groups, selecting the F3 groups of single plant is entirely The cotton plant of ornamental type cotton harvests seed, obtains F4 for seed.
Preferably, the F3 of every plant of collection is not less than 100 plants for the scale that seed is planted.
F4 generations and F5 are planted for seed, are to screen to obtain corresponding plant type.
Further, the F4 is planted for seed, and selection target flower shape and short bar and compact plant single plant mix Harvest, obtains F5 for seed.
Further, the F5 is planted for seed, and selection target flower shape and short bar and compact plant single plant are distinguished Harvest, obtains F6 for seed.
Preferably, F4 generations and/or F5 are not less than 500 plants for the scale that seed is planted.
F6 is in order to which corresponding character is further purified for seed plantation.
Further, the F6 that plant division is collected is planted for seed point group, selects the F6 groups of single plant generally short bar and plant type The cotton plant of compact target flower shape, and poor plant type single plant is eliminated, mixing harvest seed obtains F7 for seed;
The F6 of every plant of collection is not less than 100 plants for the scale that seed is planted.
Further, the F7 is planted for seed, obtains the ornamental type cotton that flower shape and plant type are stablized.
That is F7 has stable character inheritance for seed and its later seed.
It should be noted that since the character of the target flower shape of ornamental type cotton provided by the invention is recessiveness Shape, flower is wide-open after the plantation of F1 generation seed, self cannot hybridize, therefore, is selfed, obtained using human assistance F2 planted for seed after can be obtained the flower shape of trait segregation.Because the target flower shape of the present invention is or approximate Flower for windmill-shape or rosette, both flower-shapes is all endless Full-open, therefore, can be hybridized with self, just no longer be needed Want extraneous auxiliary selfing.That is, the ornamental type cotton that the present invention screens completes selfing during plantation.
In the present invention, grafts stock used and can be selected from any one cotton seed, the source of stock is not particularly limited, It such as grafts stock used and is selected from any one of following cotton seed:Nakamise 24, nakamise 36, nakamise 45, nakamise 49, in Cotton institute 53, nakamise 55, nakamise 62, nakamise 63, nakamise 65, nakamise 66, nakamise 71, nakamise 87, TM-1.
Further, the target flower shape is windmill-shape or rose.
Compared with prior art, beneficial effects of the present invention are:
(1) inventors have surprisingly discovered that, using cleisogamy material C J1548143 as female parent, the new sea of island cotton variety 21 Number be used as male parent, hybridization, harvest seed selfing, then further plantation, windmill-shape or rose or its approximate flower can be obtained The ornamental type cotton of shape, energy rapid batch produces ornamental type cotton plant by the way of grafting, meets cotton and watches the market demand.
(2) propagation method of ornamental type cotton provided by the invention is selected the mode that specific bud connects to be grafted, is easy to Mass propagation is convenient in operation.
(3) propagation method of ornamental type cotton provided by the invention grafts the ornamental type cotton of target flower shape used The tender shoots for spending the cotton seed of optional low generation to plant eliminates the step of screening distinct variety, simplifies step, saves the time, And it is easy to grasp the control of cotton seed.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below There is attached drawing needed in technology description to be briefly described.
Fig. 1 be the present embodiments relate to cleisogamy material C J1548143 flower photo;
Fig. 2 be the present embodiments relate to Xinhai 21 flower photo;
Fig. 3 is the flower photo for the windmill-shape flower cotton that the embodiment of the present invention is cultivated;
Fig. 4 is the flower photo for the rose flower cotton that the embodiment of the present invention is cultivated;
Fig. 5 is the growth conditions photo that stock used is grafted in the embodiment of the present invention;
Fig. 6 is the tender shoots photo of windmill-shape or rose flower cotton plant that the embodiment of the present invention is won;
Fig. 7 is the cotton seedling photo after grafting of the embodiment of the present invention;
Fig. 8 is the cotton seedling photo cultivated after the embodiment of the present invention is grafted two weeks.
Specific implementation mode
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is The conventional products that can be obtained by commercially available purchase.
Embodiment 1
The propagation method of windmill-shape flower cotton
1. cleisogamy material C J1548143 configures cross combination with island cotton variety Xinhai 21
Cleisogamy material C J1548143:On the day of florescence flower blooms loose powder pollination, petal closes, and does not open, complete It pollinates at self, flower is as shown in Figure 1.The material can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute or commercially available purchase obtains .
Xinhai 21:Island cotton variety, flower are as shown in Figure 2.The material can be studied from Chinese Academy of Agricultural Sciences cotton It is obtained.Precocity, high yield, high-quality long-staple cotton new varieties Xinhai 21 [J] Cottons, 2004,31 (1):31, this article has Disclose the material.
Chamber planting cleisogamy material C J1548143 and each 10 plants of Xinhai 21 in Hainan, with cleisogamy material CJ1548143 is pollinated, cotton boll maturation harvests F1 seeds as maternal, florescence emasculation with the pollen of Xinhai 21.In the greenhouse 100 plants of F1 plant are planted, selfing harvests F2 seeds.
2. selecting windmill-shape flower single plant
500 plants of F2 single plants, continuous 10 day morning 8 in florescence are planted in the greenhouse:00-12:00 selection windmill-shape flower Cotton plant finally selects 5 plants respectively, and windmill-shape flower is as shown in Figure 3.
3. stock
Nakamise 24:Upland Cotton can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute.In short season cotton new varieties- 24 [J] Cottons of cotton, 1995, (11):31.
It is planted in nutritive cube, it is spare to grow to two panels true leaf in the greenhouse, sees Fig. 5.
4. the propagation by grafiting of windmill-shape flower cotton plant
The tender shoots (see Fig. 6) for the windmill-shape flower cotton plant chosen is won with scissors, bottom is positioned in clear water, spare.With Scalpel after alcohol disinfecting is close to cotyledon upper level excision cotton seedling top in stock, then straight vertically downward in the middle part of stalk Cut the notch of 1~2cm.The tender shoots for taking out ready windmill-shape flower cotton plant, removes extra blade and stalk, only retains 1 The stem of true leaf and 3~5cm, the scalpel after disinfecting in alcohol sideling cut a knife wedgewise (1~2cm) respectively on bottom both sides. Then, the tender shoots cut is injected into stock notch, pays attention to being inserted to bottom, interface is wound repeatedly with cord, is tightened (see figure 7).It is placed in greenhouse and continues to cultivate, the cotton seedling survived after two weeks can list marketing (see Fig. 8).
Embodiment 2
The propagation method of rose flower cotton
1. cleisogamy material C J1548143 configures cross combination with island cotton variety Xinhai 21
Cleisogamy material C J1548143:On the day of florescence flower blooms loose powder pollination, petal closes, and does not open, complete It pollinates at self, flower is as shown in Figure 1.The material can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute or commercially available purchase obtains .
Xinhai 21:Island cotton variety, flower are as shown in Figure 2.The material can be studied from Chinese Academy of Agricultural Sciences cotton It is obtained.Precocity, high yield, high-quality long-staple cotton new varieties Xinhai 21 [J] Cottons, 2004,31 (1):31, this article has Disclose the material.
Chamber planting cleisogamy material C J1548143 and each 10 plants of Xinhai 21 in Hainan, with cleisogamy material CJ1548143 is pollinated, cotton boll maturation harvests F1 seeds as maternal, florescence emasculation with the pollen of Xinhai 21.In the greenhouse 100 plants of F1 plant are planted, selfing harvests F2 seeds.
2. selecting rose flower single plant
500 plants of F2 single plants, continuous 10 day morning 8 in florescence are planted in the greenhouse:00-12:The 00 rose flower of selection Cotton plant finally selects 5 plants respectively, and rose flower is as indicated at 4.
3. stock
Nakamise 24:Upland Cotton can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute.In short season cotton new varieties- 24 [J] Cottons of cotton, 1995, (11):31.
It is planted in nutritive cube, it is spare to grow to two panels true leaf in the greenhouse, sees Fig. 5.
4. the propagation by grafiting of rose flower cotton plant
The tender shoots (see Fig. 6) for the rose flower cotton plant chosen is won with scissors, bottom is positioned in clear water, spare.With Scalpel after alcohol disinfecting is close to cotyledon upper level excision cotton seedling top in stock, then straight vertically downward in the middle part of stalk Cut the notch of 1~2cm.The tender shoots for taking out ready rose flower cotton plant, removes extra blade and stalk, only retains 1 The stem of true leaf and 3~5cm, the scalpel after disinfecting in alcohol sideling cut a knife wedgewise (1~2cm) respectively on bottom both sides. Then, the tender shoots cut is injected into stock notch, pays attention to being inserted to bottom, interface is wound repeatedly with cord, is tightened (see figure 7).It is placed in greenhouse and continues to cultivate, the cotton seedling survived after two weeks can list marketing (see Fig. 8).
Embodiment 3
The propagation method of the ornamental type cotton of windmill-shape or rose flower
1. cleisogamy material C J1548143 configures cross combination with island cotton variety Xinhai 21
Cleisogamy material C J1548143:On the day of florescence flower blooms loose powder pollination, petal closes, and does not open, complete It pollinates at self, flower is as shown in Figure 1.The material can be obtained from the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute or commercially available purchase obtains .
Xinhai 21:Island cotton variety, flower are as shown in Figure 2.The material can be studied from Chinese Academy of Agricultural Sciences cotton It is obtained.Precocity, high yield, high-quality long-staple cotton new varieties Xinhai 21 [J] Cottons, 2004,31 (1):31, this article has Disclose the material.
Chamber planting cleisogamy material C J1548143 and each 20 plants of Xinhai 21 in Hainan, with cleisogamy material CJ1548143 is pollinated, cotton boll maturation harvests F1 seeds as maternal, florescence emasculation with the pollen of Xinhai 21.In the greenhouse 200 plants of F1 plant are planted, selfing harvests F2 seeds.
2. selection target flower-shape flower single plant
1000 plants of F2 single plants, continuous 10 day morning 8 in florescence are planted in the greenhouse:00-12:00 selection windmill-shape or rose Rare shape flower single plant, final choice go out 20 plants, harvest F3 seeds.
Continue to plant F3 seeds, continuous 10 day morning 8 in florescence in the greenhouse:00-12:00 selection windmill-shape or rose Flower single plant;
It is grafted using the method in embodiment 1 or embodiment 2, the cotton seedling largely grafted, you can for commercially available.
Same method after the plantation of F3 seeds, harvests seed, that is, F4 seeds with windmill-shape or rose flower character. Continue to plant F4 seeds, continuous 10 day morning 8 in florescence in the greenhouse:00-12:00 selection windmill-shape or rose flower list Strain;It is grafted using the method in embodiment 1 or embodiment 2, the cotton seedling largely grafted, you can for commercially available.
And so on, the windmill-shape or rose flower single plant screened after F5 seeds, F6 seeds, F7 seeds being planted, It is grafted using the method in embodiment 1 or embodiment 2, the cotton seedling largely grafted, you can for commercially available.
It should be noted that embodiment 1 and embodiment 2 can be carried out at the same time, it can also separate and carry out, generally be carried out at the same time i.e. It can.
Multiple a large amount of contents for repeating to implement embodiment 1-3, energy stable screening obtain ornamental type windmill-shape flower cotton Windmill cotton and the rose flower cotton rose cotton of ornamental type, illustrate that a kind of screening technique of ornamental type cotton provided by the invention is steady It is fixed effective.
Obtained ornamental type cotton ornamental type cotton by the way of grafting is screened, high-volume quickly produces ornamental type Cotton cotton plant meets cotton and watches the market demand.
In summary, the propagation method of a kind of ornamental type cotton provided by the invention, with cleisogamy material C J1548143 As female parent, island cotton variety Xinhai 21 is as male parent, and hybridization, the seed selfing of harvest, then further plantation, obtains wind Vehicle shape or rose or its approximate flower-shape ornamental type cotton, energy rapid batch produces ornamental type cotton plant by the way of grafting, Graft survival rate 100% meets cotton and watches the market demand.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from the present invention's Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims Including belonging to all such changes and modifications in the scope of the invention.

Claims (10)

1. a kind of propagation method of ornamental type cotton, which is characterized in that include the following steps:
Using cleisogamy material C J1548143 as female parent, island cotton variety Xinhai 21 harvests F1 generation as male parent, hybridization Seed;
It is selfed after the F1 generation seed plantation, F2 is for seed for harvest;
F2 is for seed or its progeny seed;
The ornamental type cotton screened is grafted as scion, and culture obtains the young plant of ornamental type cotton.
2. the propagation method of ornamental type cotton according to claim 1, which is characterized in that the grafting connects for bud.
3. the propagation method of ornamental type cotton according to claim 2, which is characterized in that the bud selecting is with growing to two The cotton stock of piece true leaf is grafted using the tender shoots of the ornamental type cotton as scion.
4. the propagation method of ornamental type cotton according to claim 2, which is characterized in that the step of bud connects is specific For:
On the cotyledon upper level excision cotton seedling top of nakamise 24, then 1~2cm of vertical cut is cut vertically downward in the middle part of stalk Mouthful;
The tender shoots of ornamental type cotton removes extra blade and stalk, retains 1 true leaf and the stem of 3~5cm, in the bottom of the stem A knife wedgewise is sideling cut respectively in both sides;
The tender shoots cut is injected into stock notch, binding is placed in greenhouse and continues to cultivate.
5. the propagation method of ornamental type cotton according to claim 4, which is characterized in that the tender shoots of the ornamental type cotton Stem using disinfection after cutter be whittled into wedge shape.
6. the propagation method of ornamental type cotton according to claim 5, which is characterized in that the length of the stem of wedge-shaped tender shoots For 1~2cm.
7. the propagation method of ornamental type cotton according to claim 1, which is characterized in that the male parent and maternal plantation Strain number is not less than 10 plants, and the strain number of F1 generation seed plantation is not less than 100 plants.
8. the propagation method of ornamental type cotton according to claim 1, which is characterized in that progeny seed is that F3 generations are above Seed, preferably F3-F5 generations in generation.
9. according to the propagation method of claim 1-8 any one of them ornamental type cottons, which is characterized in that used in the grafting Stock be selected from any one of following cotton seed:Nakamise 24, nakamise 36, nakamise 45, nakamise 49, nakamise 53, in Cotton institute 55, nakamise 62, nakamise 63, nakamise 65, nakamise 66, nakamise 71, nakamise 87, TM-1.
10. according to the propagation method of claim 1-8 any one of them ornamental type cottons, which is characterized in that the target flower Piece shape is windmill-shape or rose.
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CN112400645A (en) * 2020-11-16 2021-02-26 安徽省农业科学院棉花研究所 Method for cultivating new high-temperature-resistant cotton variety
CN112400645B (en) * 2020-11-16 2024-03-29 安徽省农业科学院棉花研究所 Cultivation method of high-temperature-resistant cotton new variety
CN113287514A (en) * 2021-07-07 2021-08-24 安徽省农业科学院棉花研究所 Propagation method of mottled leaf ornamental cotton
CN113287514B (en) * 2021-07-07 2022-11-29 安徽省农业科学院棉花研究所 Propagation method of mottled leaf ornamental cotton
CN113692933A (en) * 2021-09-24 2021-11-26 湖北省农业科学院经济作物研究所 Method for ornamental cultivation by using cotton

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