CN108467296A - A kind of composite bacterial fertilizer and preparation method thereof based on biogas slurry - Google Patents
A kind of composite bacterial fertilizer and preparation method thereof based on biogas slurry Download PDFInfo
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- CN108467296A CN108467296A CN201810239539.0A CN201810239539A CN108467296A CN 108467296 A CN108467296 A CN 108467296A CN 201810239539 A CN201810239539 A CN 201810239539A CN 108467296 A CN108467296 A CN 108467296A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
A kind of composite bacterial fertilizer and preparation method thereof based on biogas slurry belongs to biological fertilizer field.The present invention provides a kind of composite bacterial fertilizer based on biogas slurry, is made of the raw material of following mass percent for the bacterial manure formulation problems of the compound multiple beneficial microorganism based on biogas slurry：Biogas slurry 10%~50%, culture auxiliary material 0.2%~10% and complex microorganism bacterium solution 0.25%~2.5%, surplus are water；The culture auxiliary material, is mixed by sodium chloride, glucose, potassium dihydrogen phosphate, magnesium sulfate, sodium acetate, ammonium hydrogen carbonate, potassium sulfate, corn flour, soy meal, calcium carbonate and glycine；The complex microorganism bacterium solution is obtained by bacillus subtilis, lactic acid bacteria, photosynthetic bacteria and saccharomycetes to make fermentation, the complex microorganism bacterium solution a concentration of 1~2 × 108CFU/ml.It is added in fermentation vat after biogas slurry, culture auxiliary material and water are mixed in proportion, then complex microorganism bacterium solution is accessed to simultaneously mixing in proportion, fermentation obtains composite bacterial fertilizer.The present invention is suitable for the development and utilization of microbial-bacterial fertilizer.
Description
Technical field
The composite bacterial fertilizer and preparation method thereof that the present invention relates to a kind of based on biogas slurry, belongs to biological fertilizer field.
Background technology
Biogas slurry is the liquid that the brown formed after organic substance is fermented becomes clear, and is the byproduct of biogas production, containing more
Kind amino acid and trace element, are a kind of good feed addictives.Biogas slurry not only contains a large amount of battalion such as abundant nitrogen, phosphorus, potassium
The micronutrient elements such as element and zinc are supported, and contain 17 kinds of amino acid, organized enzymes.These nutrients are substantially with quick-acting
Existing for nutrient form, therefore, the quick-acting nutritious ability of biogas slurry is strong, and Nutrient availability is high, is polynary quick-acting compound fertilizers,
It can be absorbed and utilized by animal and crops rapidly.
Commercialized culture fishery mostly based on high density, intensive culture, such production model bring it is huge
While economic interests, many problems are also produced, such as a large amount of residual baits and excrement enter water body, cause body eutrophication tight
Weight, water quality deterioration, disease take place frequently.For prevention and control disease occurred frequently, a large amount of chemicals and antibiotics are often used in production,
Although these drugs have certain inhibition and therapeutic effect to breaking out for disease, from a long-term perspective, to aquaculture
The sound development of industry is totally unfavorable, is mainly manifested in：Due to these drugs meeting polluted-water, beneficial microbe in water is murdered,
Destroy water ecological setting；Medicament residue can be also caused, aquatic products is made to reduce or lose economic value；Antibiotics
It is a large amount of to use, also easily pathogen is caused to develop immunity to drugs, while reducing therapeutic effect, can directly endanger the strong of the mankind
Health.
By retrieval, it is found that following 5 documents are related to the application：1, a kind of edible mushroom parent species marsh liquid culture medium and
Parent species production method (CN200810058331.5), 2, a kind of in method that biogas slurry is basic culture medium fermenting and producing organic fertilizer
(CN201110124953.5), 3, a kind of biogas slurry organic nutrient solution for cultivating and its production method with stability
(CN201010259669.4), a kind of 4, method using biogas slurry anaerobic shake culture of photosynthetic bacteria
(CN201210467930.9), a kind of 5, method (CN201110430901.0) using biogas slurry culture photosynthetic bacteria.1st is
Field of edible fungus culture discloses a kind of application of single culture-edible mushroom in biogas slurry culture, and the 2nd, 3 is all single bacterium
Kind-bacillus is applied to the growth of crops in the culture of biogas slurry, and the 4th, 5 is photosynthetic using biogas slurry culture single culture-
Bacterium.
For current worsening water body, merely using biogas slurry carry out aquaculture cannot meet water body optimization with
Various demands such as increase production and improve productivity, and the composite fermentation technology that multiple beneficial microorganism species are carried out using biogas slurry is more tired
It is difficult.
Invention content
The present invention provides one kind with natural pond for the bacterial manure formulation problems of the compound multiple beneficial microorganism based on biogas slurry
Composite bacterial fertilizer based on liquid, the bacterial manure are made of the raw material of following mass percent：Biogas slurry 10%~50%, culture auxiliary material
0.2%~10% and complex microorganism bacterium solution 0.25%~2.5%, surplus be water；The culture auxiliary material, by following quality percentage
The raw material of ratio forms：Sodium chloride 0.2%~3%, glucose 1.5%~20%, potassium dihydrogen phosphate 0.1%~2.5%, magnesium sulfate
0.05%~1%, sodium acetate 0.3%~8%, ammonium hydrogen carbonate 0.05%~2%, potassium sulfate 0.1%~2%, corn flour 1%~
20%, soy meal 1%~35%, calcium carbonate 2%~28% and glycine 0.05%~1%；The complex microorganism bacterium solution, by
The fermenting raw materials of following mass percent obtain：Bacillus subtilis 20%~60%, lactic acid bacteria 10%~30%, photosynthetic bacteria
25%~45% and saccharomycete 10%~40%, the sum of each Material Percentage is 100%, after each bacterium solution fermentation beneficial to micro-
Biologic live bacteria number is respectively 1~2 × 108CFU/ml。
Preferably, the bacterial manure is made of the raw material of following mass percent：Biogas slurry 50% is cultivated auxiliary material 2.5% and is answered
Microbial inoculum 0.5% is closed, surplus is water；The culture auxiliary material, is made of the raw material of following mass percent：Sodium chloride 1%,
Glucose 20%, potassium dihydrogen phosphate 0.28%, magnesium sulfate 1%, sodium acetate 6%, ammonium hydrogen carbonate 0.2%, potassium sulfate 1%, corn
Powder 20%, soy meal 35%, calcium carbonate 14.52% and glycine 1%；The complex microorganism bacterium solution, by following quality percentage
The fermenting raw materials of ratio obtain：Bacillus subtilis 20%, lactic acid bacteria 30%, photosynthetic bacteria 25% and saccharomycete 25%, it is described each
The total viable count of beneficial microbe 5 × 10 after bacterium solution fermentation8CFU/ml or more.
Preferably, the residue that the biogas slurry is biogas after anaerobic fermentation 1 month, after filtering, removing solid impurity
Filtrate, the filtering refers to that residue crosses 50 mesh dusting covers.
Preferably, the fermentation of each bacterium solution refers to first by bacillus subtilis, lactic acid bacteria, photosynthetic bacteria and saccharomycete bacterium
Kind is inoculated into seed tank culture respectively, ferments after inoculating fermentation tank mixing.
Preferably, the lactic acid bacteria is plant lactobacillus, and the photosynthetic bacteria is Rhodopseudomonas palustris, the saccharomycete
For saccharomyces cerevisiae.
The preparation method for the composite bacterial fertilizer that the present invention also provides above-mentioned based on biogas slurry：By biogas slurry, culture auxiliary material and
Water is added after mixing in proportion in fermentation vat, then complex microorganism bacterium solution accessed in proportion, and at 25~30 DEG C, intensity of illumination is
Under the conditions of 400~2000 luxs, ferments 3~5 days and obtain composite bacterial fertilizer.
Preferably, after biogas slurry, culture auxiliary material and water mixs in proportion in addition fermentation vat, then by complex microorganism bacterium solution
It accesses in proportion, at 28 DEG C, intensity of illumination is 3 days acquisition composite bacterial fertilizers of fermentation under the conditions of 1000 luxs.
Advantageous effect
1. biogas slurry promotes the mechanism of fish growth mainly to have two aspects：
(1) phytoplankton absorbs inorganic nutrients in biogas slurry, so that its own is obtained mass propagation in sunlight presence.It swims plant
Object can be directly as the bait of filter-feeding fish.It can be ingested again by zooplankter, zooplankter is made to be grown and be bred.Afterwards
Person is finally the bait of filter-feeding fish again.
(2) in aquatic ecosystem, microorganism is to connect the bridge for producing and decomposing between abiotic factor and living nature
Beam.In fresh water aquaculture system, microbial activities has the meaning of bigger.When anaerobic fermentation residues detach, Shao Liangzhao
Slag remains in biogas slurry, the nutrient generated after the aerobic and anaerobic digestion that biogas residue passes through bacterium and bacterium can by fish profit
With.Bacterium in addition to being ingested by zooplankter and water body bottom toy of dwelling, and then by fish outside, also can be directly by fish institute
It ingests.
And utilize probiotics made of microorganism species beneficial in environment or aquaculture organism body, both had no toxic side effect with it is residual
Problem is stayed, and growth can be promoted, prevented and cured diseases, can also improve the environment of breeding water body.Breeded fish using biogas slurry, can save cultivation at
This, improves the production performance of beasts, birds and aquatic products.Biogas slurry can increase fish pond planktonic organism amount after entering fish pond, reinforce photosynthesis, increase
Add oxygen-producing amount, reduce fish disease, save chemical fertilizer, bait etc..
It is directed to aquaculture field, the factor for influencing Water quality is more, the water body under different zones and varying environment
Difference is larger, cannot play good effect containing single beneficial microorganism biogas slurry, how successfully to utilize biogas slurry culture beneficial
Bacterium becomes key, and the speed of growth of each bacterium is different, and fermentation condition is different, and nutritional ingredient difference, the ion concentration of preference are different
Factors different with PH etc., it is desirable to make the fast-growth of each bacterium simultaneously, synchronous, balanced be nearly impossible, therefore compound
The selection of the adding ingredient of bacterium culture medium and ratio, i.e. biogas slurry cultivate the ratio of auxiliary material and water, and the quality of each bacterium accounts in compound bacteria
Than being very crucial, the growth demand of strain can either be met simultaneously, and can be taken according to the difference of each bacterium speed of growth
Mix crucial thalline inoculative proportion so that the strain lactic acid bacteria grown at first to the utilization of medium component and culture environment and
Transformation, disclosure satisfy that and quickly grown conducive to other strains, to achieve the purpose that and complete the growth of compound bacteria fast uniform.
2. the present invention based on biogas slurry, using aquaculture and improves four kinds of beneficial microbes of water quality, i.e. withered grass bud
Spore bacillus, lactic acid bacteria, photosynthetic bacteria and saccharomycete are prepared for a kind of composite bacterial fertilizer and preparation method thereof, after fermenting in the bacterial manure
The total viable count of compound beneficial microbe up to 5 × 108CFU/ml or more, each microorganism can jointly quickly, uniform growth, the hair
Fermenting process is simple and practicable, and culture medium cost of supplementary product used is cheap, and fermentation process is not high to equipment requirement, is very suitable for industrializing
Large-scale promotion.
Specific implementation mode
Bacillus subtilis used in following each embodiments is purchased from Chinese industrial Microbiological Culture Collection administrative center, withered grass
Bacillus (Bacillus subtilis), bacterial strain deposit number：CICC No.10089.
Lactic acid bacteria used is purchased from Chinese industrial Microbiological Culture Collection administrative center, is lactobacillus plantarum
(Lactobacillus plantarum), bacterial strain deposit number：CICC No.21794.
Photosynthetic bacteria used is purchased from Chinese industrial Microbiological Culture Collection administrative center, is Rhodopseudomonas palustris
(Rhodopseudomonas palustris), bacterial strain deposit number：CICC No.23812.
Used yeast bacterium is purchased from Chinese industrial Microbiological Culture Collection administrative center, is saccharomyces cerevisiae (Saccharomyces
Cerevisiae), bacterial strain deposit number：CICC No.1510.
The methane-generating pit is the methane-generating pit of conventional cylinder shape belt stirring.
It is conventional method that strain described in following each embodiments, which carries out seed tank culture method,:
The condition of fermentation tank culture is:
Activated bacillus subtilis is inoculated in the triangular flask of SDAY culture mediums, carries out shaking flask culture, SDAY trainings
The liquid amount for supporting base is the 20~50% of triangular flask volume, 180~200r/min shaken cultivations 16~for 24 hours at 28~30 DEG C,
Obtain primary seed solution, by primary seed solution be added equipped with SDAY culture mediums triangular flask, inoculum concentration be SDAY culture mediums 3~
The liquid amount of 5%v/v, SDAY culture medium is the 20~50% of triangular flask volume, and 180~200r/min vibrates at 28~30 DEG C
Culture 16~for 24 hours, secondary seed solution is obtained, spare fermentation medium is added in stirred-tank fermenter, fermentation medium
Liquid amount is the 50~60% of fermenter volume, and 0.09~0.11%w/v antifoaming agent accesses secondary seed solutions by 5~10%,
3~5L/min of ventilatory capacity at 28~30 DEG C, tank are pressed 60~80kPa, are cultivated under the conditions of 180~220r/min of speed of agitator, cultivated
Time is 16h~for 24 hours, obtains three-level seed liquor.
By activated lactobacillus inoculum in MRS fluid nutrient mediums, 37 DEG C of 15~20h of culture using it are seed according to 3
~5% inoculum concentration is linked into 15~30kg of sucrose, 15~30kg of yeast powder, 7~15kg of peptone, composite buffering 1~3L of liquid,
Compound 0.1~0.3L of inorganic salt solution in the culture medium of distilled water 1000L, carries out fermentation tank culture, 30~37 DEG C of constant temperature trainings
It supports, in fermenting to 2~6h, the method for the ammonium hydroxide that addition mass percent concentration is 25% controls fermentation process pH and keeps constant
5.5~5.8,8~10h is cultivated.
Activated photosynthetic bacteria is inoculated by the inoculum concentration of 5-10% in culture medium, which is：NH4SO4
0.5-l.5g；KH2P040.05-0.5g CH3COONa 1.5-3.0g MgSO4·7H2O 0.25-1.0g NaCl 1.0~
3.0g；Yeast extract 0.5~l.0g；Tween-80 0.2-lg；1000.0ml, PH=7.0~7.5,121 degree of tap water sterilizes 15 points
The above-mentioned culture solution for having connect strain is placed in transparent container, then sealing container by clock postcooling to 30 DEG C, temperature 30~
35 DEG C, illumination cultivation 5~6 days under the conditions of 1500~4000LX of intensity of illumination, solution becomes peony.
Activated saccharomycete is inoculated in malt juice liquid medium, 28~30 DEG C, 200~250r/min, shaking flask
Overnight incubation obtains seed liquor, and 75.80~77.12g/L of sucrose, yeast extract are accessed according still further to 8~10% inoculum concentration
33.31~34.25g/L, sour 15.86~16.15g/L of hydrolyzed casein, ammonium sulfate 0.55g/L, magnesium sulfate 1.03g/L, di(2-ethylhexyl)phosphate
In the culture medium of hydrogen potassium 2.62g/L and glycine 2.03g/L, using 28~30 DEG C, 200~250r/min conditions carry out fermentation tank
Culture starts to mend sugar when cultivating to 16h, mends sucrose solution of the sugar using a concentration of 150g/ml prepared in advance, is mended every 12h
It is sugared primary, 1.3~1.6ml, 60~72h of fermented and cultured are filled into every time.
The preparation method of the composite bacterial fertilizer based on biogas slurry is detailed below.
The preparation method of composite bacterial fertilizer of the embodiment 1. based on biogas slurry.
(1) residue of the biogas through anaerobic fermentation 1 month or more in the methane-generating pit of normal operation, through filtering, removing solid
Biogas slurry 50kg after impurity, it is spare.
(2) sodium chloride 25g, glucose 500g, potassium dihydrogen phosphate 7g, magnesium sulfate 25g, sodium acetate 150g, bicarbonate are weighed
Ammonium 5g, potassium sulfate 25g, corn flour 500g, soy meal 875g, calcium carbonate 363g and glycine 25g after mixing, are prepared and are completed
2.5kg cultivates auxiliary material, spare.
(3) by bacillus subtilis, lactobacillus plantarum, Rhodopseudomonas palustris and saccharomyces cerevisiae, respectively according to a conventional method
By being inoculated into seed tank culture, bacterium solution is obtained after inoculating ferment tank, bacteria concentration is respectively 2 × 108CFU/ml, amount
Take bacillus subtilis bacterium solution 100ml, lactobacillus plantarum bacterium solution 150ml, Rhodopseudomonas palustris bacterium solution 125ml and saccharomyces cerevisiae
Bacterium solution 125ml has prepared 500ml complex microorganism bacterium solutions after mixing, and bacterium solution quality is spare based on 500g.
It is added in fermentation vat after 50kg biogas slurries, the culture auxiliary material of 2.5kg and 47kg water are mixed, while again answering 500ml
Microbial inoculum access and mixing are closed, at 28 DEG C, intensity of illumination is 1000 luxs, ferments 3 days, obtains training based on biogas slurry
Product, i.e. composite bacterial fertilizer after the culture solution fermentation of foster complex microorganism.
4 kinds of beneficial microorganism cell concentrations in product, are shown in Table 1 after detection fermentation.
Table 1. cultivated based on biogas slurry complex microorganism culture solution fermentation after each beneficial microbe cell concentration of product
| Beneficial microbe title | Cell density (CFU/ml) |
| Bacillus subtilis | 1.5×108 |
| Lactobacillus plantarum | 2.1×108 |
| Rhodopseudomonas palustris | 2.8×108 |
| Saccharomyces cerevisiae | 1.9×108 |
Application effect：Product 10kg of the present invention is taken once to send out 3 mu, the crucian pond that about 3 meters of the depth of water, with the pond that do not apply
By contrast, water quality is obviously improved on the pool, and fish incidence can reduce by 10% or so.
The preparation method of composite bacterial fertilizer of the embodiment 2. based on biogas slurry.
(1) residue of the biogas through anaerobic fermentation 1 month or more in the methane-generating pit of normal operation, through filtering, removing solid
Biogas slurry 25kg after impurity, it is spare.
(2) sodium chloride 0.25kg, glucose 1.86kg, potassium dihydrogen phosphate 0.2kg, magnesium sulfate 8g, sodium acetate are weighed
0.8kg, ammonium hydrogen carbonate 0.2kg, potassium sulfate 0.15kg, corn flour 1.8kg, soy meal 2.1kg, calcium carbonate 2.55kg and glycine
82g after mixing, is prepared and is completed 10kg culture auxiliary materials, spare.
(3) by bacillus subtilis, lactobacillus plantarum, Rhodopseudomonas palustris and saccharomyces cerevisiae, respectively according to a conventional method
By being inoculated into seed tank culture, the bacterium solution obtained after ferment tank is inoculated, bacteria concentration is respectively 1 × 108CFU/ml,
Measure bacillus subtilis bacterium solution 300ml, lactobacillus plantarum bacterium solution 200ml, Rhodopseudomonas palustris bacterium solution 200ml and wine brewing ferment
Female bacterium solution 300ml has prepared 1L complex microorganism bacterium solutions after mixing, and bacterium solution quality is spare based on 1kg.
It is added in fermentation vat after 25kg biogas slurries, the culture auxiliary material of 10kg and 64kg water are mixed, while again that 1L is compound micro-
Biological bacterium solution access and mixing, at 30 DEG C, intensity of illumination is 800 luxs, is fermented 4 days, obtains cultivating based on biogas slurry multiple
Product, i.e. composite bacterial fertilizer after the culture solution fermentation of conjunction microorganism.
4 kinds of beneficial microorganism cell concentrations in product, are shown in Table 2 after detection fermentation.
Table 2. cultivated based on biogas slurry complex microorganism culture solution fermentation after each beneficial microbe cell concentration of product
| Beneficial microbe title | Cell density (CFU/ml) |
| Bacillus subtilis | 2.5×108 |
| Lactobacillus plantarum | 1.8×108 |
| Rhodopseudomonas palustris | 2.3×108 |
| Saccharomyces cerevisiae | 1.5×108 |
Application effect：The product 5kg of the present invention is taken once to send out 4 mu, the fresh water shrimp pond of the depth of water about 1.5m and is not applied
By contrast, water quality is obviously improved in pond, and year harvests 3000 jin of shrimp, output increased 5% or so.
The preparation method of composite bacterial fertilizer of the embodiment 3. based on biogas slurry.
(1) residue of the biogas through anaerobic fermentation 1 month or more in the methane-generating pit of normal operation, through filtering, removing solid
Biogas slurry 10kg after impurity, it is spare.
(2) sodium chloride 140g, glucose 1kg, potassium dihydrogen phosphate 70g, magnesium sulfate 40g, sodium acetate 15g, bicarbonate are weighed
Ammonium 10g, potassium sulfate 50g, corn flour 1kg, soy meal 1.25kg, calcium carbonate 1.4kg and glycine 25g after mixing, are prepared
It completes 5kg and cultivates auxiliary material, it is spare.
(3) by bacillus subtilis, lactobacillus plantarum, Rhodopseudomonas palustris and saccharomyces cerevisiae, respectively according to a conventional method
By being inoculated into seed tank culture, the bacterium solution obtained after ferment tank is inoculated, bacteria concentration is respectively 1.5 × 108CFU/
Ml measures bacillus subtilis bacterium solution 1L, lactobacillus plantarum bacterium solution 0.2L, Rhodopseudomonas palustris bacterium solution 0.5L and saccharomyces cerevisiae
Bacterium solution 0.3L has prepared 2L complex microorganism bacterium solutions after mixing, and bacterium solution quality is spare based on 2kg.
It is added in fermentation vat after 10kg biogas slurries, the culture auxiliary material of 5kg and 83kg water are mixed, while again by 2L composite microbials
The access of object bacterium solution and mixing, at 25 DEG C, intensity of illumination is 1200 luxs, ferments 5 days, obtains cultivating based on biogas slurry compound
Product, i.e. composite bacterial fertilizer after the culture solution fermentation of microorganism.
4 kinds of beneficial microorganism cell concentrations in product, are shown in Table 3 after detection fermentation.
Table 3. cultivated based on biogas slurry complex microorganism culture solution fermentation after each beneficial microbe cell concentration of product
| Beneficial microbe title | Cell density (CFU/ml) |
| Bacillus subtilis | 2.5×108 |
| Lactobacillus plantarum | 1.9×108 |
| Rhodopseudomonas palustris | 2.1×108 |
| Saccharomyces cerevisiae | 1.7×108 |
The preparation method of composite bacterial fertilizer of the embodiment 4. based on biogas slurry.
(1) residue of the biogas through anaerobic fermentation 1 month or more in the methane-generating pit of normal operation, through filtering, removing solid
Biogas slurry 40kg after impurity, it is spare.
(2) weigh sodium chloride 6g, glucose 32g, potassium dihydrogen phosphate 5g, magnesium sulfate 2g, sodium acetate 12g, ammonium hydrogen carbonate 2g,
Potassium sulfate 4g, corn flour 36g, soy meal 48g, calcium carbonate 52g and glycine 1g after mixing, it is auxiliary to prepare completion 200g cultures
Material, it is spare.
(3) by bacillus subtilis, lactobacillus plantarum, Rhodopseudomonas palustris and saccharomyces cerevisiae, respectively according to a conventional method
By being inoculated into seed tank culture, the bacterium solution obtained after ferment tank is inoculated, bacteria concentration is respectively 1.25 × 108CFU/
Ml, measure bacillus subtilis bacterium solution 0.3L, lactobacillus plantarum bacterium solution 0.45L, Rhodopseudomonas palustris bacterium solution 0.6L and wine brewing
Yeast liquid 0.15L has prepared 1.5L complex microorganism bacterium solutions after mixing, and bacterium solution quality is spare based on 1.5kg.
It is added in fermentation vat after 40kg biogas slurries, the culture auxiliary material of 200g and 58.3kg water are mixed, while again answering 1.5L
Microbial inoculum access and mixing are closed, at 27 DEG C, intensity of illumination is 1600 luxs, ferments 5 days, obtains training based on biogas slurry
Product, i.e. composite bacterial fertilizer after the culture solution fermentation of foster complex microorganism.
4 kinds of beneficial microorganism cell concentrations in product, are shown in Table 4 after detection fermentation.
Table 4. cultivated based on biogas slurry complex microorganism culture solution fermentation after each beneficial microbe cell concentration of product
| Beneficial microbe title | Cell density (CFU/ml) |
| Bacillus subtilis | 2.1×108 |
| Lactobacillus plantarum | 2.0×108 |
| Rhodopseudomonas palustris | 2.9×108 |
| Saccharomyces cerevisiae | 1.3×108 |
The preparation method of composite bacterial fertilizer of the embodiment 5. based on biogas slurry.
(1) residue of the biogas through anaerobic fermentation 1 month or more in the methane-generating pit of normal operation, through filtering, removing solid
Biogas slurry 30kg after impurity, it is spare.
(2) sodium chloride 7.5g, glucose 200g, potassium dihydrogen phosphate 1g, magnesium sulfate 5g, sodium acetate 33g, ammonium hydrogen carbonate are weighed
15g, potassium sulfate 18g, corn flour 200g, soy meal 240g, calcium carbonate 280g and glycine 0.5g after mixing, are prepared and are completed
1kg cultivates auxiliary material, spare.
(3) by bacillus subtilis, lactobacillus plantarum, Rhodopseudomonas palustris and saccharomyces cerevisiae, respectively according to a conventional method
By being inoculated into seed tank culture, the bacterium solution obtained after ferment tank is inoculated, bacterial concentration is respectively 1.75 ×
108CFU/ml measures bacillus subtilis bacterium solution 0.625L, lactobacillus plantarum bacterium solution 0.625L, Rhodopseudomonas palustris bacterium solution
0.625L and saccharomyces cerevisiae bacterium solution 0.625L has prepared 2.5L complex microorganism bacterium solutions after mixing, bacterium solution quality based on 2.5kg,
It is spare.
It is added in fermentation vat after 30kg biogas slurries, the culture auxiliary material of 1kg and 66.5kg water are mixed, while again that 2.5L is compound
Microbial inoculum access and mixing, at 26 DEG C, intensity of illumination is 2000 luxs, ferments 3 days, obtains cultivating based on biogas slurry
Product, i.e. composite bacterial fertilizer after the culture solution fermentation of complex microorganism.
After detection fermentation 4 in product in beneficial microorganism cell concentration be shown in Table 5.
Table 5. cultivated based on biogas slurry complex microorganism culture solution fermentation after each beneficial microbe cell concentration of product
| Beneficial microbe title | Cell density (CFU/ml) |
| Bacillus subtilis | 1.9×108 |
| Lactobacillus plantarum | 1.7×108 |
| Rhodopseudomonas palustris | 1.5×108 |
| Saccharomyces cerevisiae | 2.2×108 |
The preparation method of composite bacterial fertilizer of the embodiment 6. based on biogas slurry.
(1) residue of the biogas through anaerobic fermentation 1 month or more in the methane-generating pit of normal operation, through filtering, removing solid
Biogas slurry 20kg after impurity, it is spare.
(2) sodium chloride 15g, glucose 1.5kg, potassium dihydrogen phosphate 109.5g, magnesium sulfate 3.75g, sodium acetate are weighed
472.5g, ammonium hydrogen carbonate 105.75g, potassium sulfate 7.5g, corn flour 1.5kg, soy meal 1.875kg, calcium carbonate 1.896 and sweet ammonia
Sour 15g after mixing, is prepared and is completed 7.5kg culture auxiliary materials, spare.
(3) by bacillus subtilis, lactobacillus plantarum, Rhodopseudomonas palustris and saccharomyces cerevisiae, respectively according to a conventional method
By being inoculated into seed tank culture, the bacterium solution obtained after ferment tank is inoculated, bacteria concentration is respectively 1.75 × 108CFU/
Ml measures bacillus subtilis bacterium solution 87.5ml, lactobacillus plantarum bacterium solution 25ml, Rhodopseudomonas palustris bacterium solution 62.5ml and wine
Brewer yeast bacterium solution 75ml has prepared 250ml complex microorganism bacterium solutions after mixing, and bacterium solution quality is spare based on 250g.
It is added in fermentation vat after 20kg biogas slurries, the culture auxiliary material of 7.5kg and 72.25kg water are mixed, while again by 250ml
The access of complex microorganism bacterium solution and mixing, at 30 DEG C, intensity of illumination is 1800 luxs, ferments 4 days, obtains based on biogas slurry
Product, i.e. composite bacterial fertilizer after the culture solution fermentation of culture complex microorganism.
4 kinds of beneficial microorganism cell concentrations in product, are shown in Table 6 after detection fermentation.
Table 6. cultivated based on biogas slurry complex microorganism culture solution fermentation after each beneficial microbe cell concentration of product
| Beneficial microbe title | Cell density (CFU/ml) |
| Bacillus subtilis | 1.5×108 |
| Lactobacillus plantarum | 1.2×108 |
| Rhodopseudomonas palustris | 1.2×108 |
| Saccharomyces cerevisiae | 1.8×108 |
Further verify the application effect of the composite bacterial fertilizer of the present invention based on biogas slurry：
Comparative example 1. prepares a kind of composite bacillus subtilis culture solution based on biogas slurry.This comparative example and embodiment
Unlike 1, microorganism is determined as single culture bacillus subtilis (Bacillus subtillis (Bacillus in this comparative example
Subtilis), bacterial strain deposit number：CICC No.10089), remaining method is identical with embodiment 1, withered after culture solution fermentation
The cell concentration of careless bacillus is 2.2 × 104。
Comparative example 2. prepares a kind of compound lactobacillus culture solution based on biogas slurry.This comparative example is different from embodiment 1
, microorganism is determined as single culture lactic acid bacteria (lactobacillus plantarum (Lactobacillus in this comparative example
Plantarum), bacterial strain deposit number：CICC No.21794), remaining method is identical with embodiment 1, breast after culture solution fermentation
The cell concentration of sour bacterium Y is 1.2 × 105。
Comparative example 3. prepares a kind of Composite Photosynthetic Bacteria culture solution based on biogas slurry.This comparative example and embodiment 1 are not
With microorganism is determined as single culture photosynthetic bacteria (Rhodopseudomonas palustris (Rhodopseudomonas in this comparative example
), palustris bacterial strain deposit number：CICC No.23812), remaining method is identical with embodiment 1, light after culture solution fermentation
The cell concentration for closing bacterium Z is 4.8 × 103。
Comparative example 4. prepares a kind of composite yeast bacteria culture fluid based on biogas slurry.This comparative example is different from embodiment 1
, in this comparative example microorganism be determined as single culture saccharomycete (saccharomyces cerevisiae (Saccharomyces cerevisiae),
Bacterial strain deposit number：CICC No.1510), remaining method is identical with embodiment 1, and the thalline of saccharomycete is dense after culture solution fermentation
Degree is 3.2 × 105。
Comparative example 1-4 and composite bacterial fertilizer described in embodiment 1 are taken into 5kg respectively, once send out 4 mu, the depth of water about 1.5m
Fresh water shrimp pond by contrast with the pond do not applied, investigate each pond Prawn Survival after raising 30 days, the results are shown in Table
7。
The influence of the different bacterial manure application processing prawn growing states of table 7.
| Group | Put body length/cm of shrimp in a suitable place to breed | Growth rate % | Survival rate % |
| Embodiment 1 | 0.5 | 105 | 70 |
| Comparative example 1 | 0.5 | 101 | 68 |
| Comparative example 2 | 0.5 | 103 | 64 |
| Comparative example 3 | 0.5 | 104 | 67 |
| Comparative example 4 | 0.5 | 102 | 65 |
| Any fertilizer is not applied in control- | 0.5 | 100 | 60 |
Claims (7)
1. a kind of composite bacterial fertilizer based on biogas slurry, which is characterized in that the bacterial manure by following mass percent raw material system
At：Biogas slurry 10%~50%, culture auxiliary material 0.2%~10% and complex microorganism bacterium solution 0.25%~2.5%, surplus are water；
The culture auxiliary material, is made of the raw material of following mass percent：Sodium chloride 0.2%~3%, glucose 1.5%~20%, phosphorus
Acid dihydride potassium 0.1%~2.5%, magnesium sulfate 0.05%~1%, sodium acetate 0.3%~8%, ammonium hydrogen carbonate 0.05%~2%,
Potassium sulfate 0.1%~2%, corn flour 1%~20%, soy meal 1%~35%, calcium carbonate 2%~28% and glycine
0.05%~1%；The complex microorganism bacterium solution is obtained by the fermenting raw materials of following mass percent：Bacillus subtilis
20%~60%, lactic acid bacteria 10%~30%, photosynthetic bacteria 25%~45% and saccharomycete 10%~40%, each Material Percentage
The sum of be 100%, the beneficial microbe viable count after each bacterium solution fermentation is respectively 1~2 × 108CFU/ml。
2. a kind of composite bacterial fertilizer based on biogas slurry according to claim 1, which is characterized in that the bacterial manure is by following
The raw material of mass percent is made：Biogas slurry 50%, culture auxiliary material 2.5% and complex microorganism bacterium solution 0.5%, surplus are water；Institute
Culture auxiliary material is stated, is made of the raw material of following mass percent：Sodium chloride 1%, glucose 20%, potassium dihydrogen phosphate 0.28%,
Magnesium sulfate 1%, sodium acetate 6%, ammonium hydrogen carbonate 0.2%, potassium sulfate 1%, corn flour 20%, soy meal 35%, calcium carbonate
14.52% and glycine 1%；The complex microorganism bacterium solution is obtained by the fermenting raw materials of following mass percent：Withered grass gemma
Bacillus 20%, lactic acid bacteria 30%, photosynthetic bacteria 25% and saccharomycete 25%, the total viable bacteria of beneficial microbe after each bacterium solution fermentation
Number 5 × 108CFU/ml or more.
3. a kind of composite bacterial fertilizer based on biogas slurry according to claim 1 or 2, which is characterized in that the biogas slurry is
Residue of the biogas after anaerobic fermentation 1 month, the filtrate after filtering, removing solid impurity.
4. a kind of composite bacterial fertilizer based on biogas slurry according to claim 3, which is characterized in that it is described filtering refer to by
Residue crosses 50 mesh dusting covers.
5. a kind of composite bacterial fertilizer based on biogas slurry according to claim 1 or 2, which is characterized in that each bacterium solution
Fermentation refer to bacillus subtilis, lactic acid bacteria, photosynthetic bacteria and yeast seeds are first inoculated into seed tank culture respectively, then
It ferments after being inoculated into fermentation tank mixing.
6. the preparation method of the composite bacterial fertilizer as claimed in claim 1 or 2 based on biogas slurry, which is characterized in that by biogas slurry, training
It supports after auxiliary material and water mixs in proportion in addition fermentation vat, then complex microorganism bacterium solution is accessed in proportion, at 25~30 DEG C, light
It is 3~5 days acquisition composite bacterial fertilizers of fermentation under the conditions of 400~2000 luxs according to intensity.
7. the preparation method of the composite bacterial fertilizer according to claim 6 based on biogas slurry, which is characterized in that by biogas slurry,
After culture auxiliary material and water mixs in proportion in addition fermentation vat, then complex microorganism bacterium solution accessed in proportion, at 28 DEG C, illumination
Intensity is 3 days acquisition composite bacterial fertilizers of fermentation under the conditions of 1000 luxs.
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