CN108432535A - Sweet osmanthus long flowering period hybrid selection method - Google Patents

Sweet osmanthus long flowering period hybrid selection method Download PDF

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CN108432535A
CN108432535A CN201810218399.9A CN201810218399A CN108432535A CN 108432535 A CN108432535 A CN 108432535A CN 201810218399 A CN201810218399 A CN 201810218399A CN 108432535 A CN108432535 A CN 108432535A
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osmanthus
weight
parts
cinnabar
soil
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CN108432535B (en
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陈振华
翁灵巍
陈争
柳宽严
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Zhejiang Lulong Ecological Science And Technology Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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Abstract

The present invention provides sweet osmanthus long flowering period hybrid selection methods, include the following steps:1) soil pre-modified, draining and delivery system structure spray microbial inoculum using soil improvement fertilizer;2) it cross combination selection and cultivates, by hybridizing for cinnabar osmanthus and day fragrant osmanthus, the flowering time of the osmanthus fragrans of new generation of generation shifts to an earlier date than normal cinnabar osmanthus, longer blooming period, aromatic flavour, and resistance is strong.

Description

Sweet osmanthus long flowering period hybrid selection method
Technical field
The present invention relates to sweet osmanthus long flowering period hybrid selection methods.
Background technology
Sweet osmanthus category long-day plant likes in sufficient sunlight and soil layer plumpness, well-drained grown, and soil wants micro- Acid, PH5.5-6.5.Avoid alkaline earth, is afraid of ponding and coal smoke, wherein cinnabar osmanthus is orange osmanthus Breeds, and arbor is 5 meters high, 3 meters of filling, tree It is preced with spheroidal, tight ness rating is medium, and bark is light grey, and smoother, hole skin is sparse, florescence August, leads over autumn osmanthus class, premium color, 4-6 millimeters of petal has colored 6-8 per bud, and flower amount is big, and flower taste is denseer, and at 15 days or so florescence, shade tolerance is poor, because of cinnabar sweet osmanthus Color is dense, and rich and honour state foot, welcomed by the people, improvement cinnabar osmanthus has compared with larger market prospect;Day fragrant osmanthus is sweet osmanthus (sweet-scented osmanthus) Mutation is gained the name with constantly blooming and giving off a strong fragrance, September to 8 months continuous florescence of April next year or more, and the 5-8 months are monthly still Have 1-2 times and bloom, substantially throughout the year constantly flower, resistance is strong, but pattern is thin, day fragrant osmanthus and cinnabar osmanthus hybridization combination new product Kind does not occur in market.
Invention content
In view of this, the object of the present invention is to provide sweet osmanthus long flowering period hybrid selection method, applied widely, osmanthus of new generation Florescence is long, thick flavor, and resistance is strong.
In order to solve the above technical problems, the technical scheme is that:
Sweet osmanthus long flowering period hybrid selection method, includes the following steps:
1) soil pre-modified
Predetermined 20-40 centimeters of the cultivation ground of step 1, draining and delivery system structure, first deep ploughing, it is smooth, it is thin with soil It is broken, subject to apparent bulk;Then it carries out canopy constituency to build, the excavation of bright ditch, the laying of seepage pipe, hidden pipe and collector pipe is bright Ditch, hidden pipe and collector pipe are connected to, for controlling groundwater level and supply nutrition;
Step 2, it is sub- using soil improvement fertilizer, including pig manure, activated carbon, humic acid, furfural dregs, calcium dihydrogen phosphate, sulfuric acid Iron, water-retaining agent and composite fertilizer, rotary tillage after applying every time, spray microbial inoculum, including nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, antibiotic bacteria, hair Yeast-like fungi etc., cycle are multiple;
2) cross combination selection and cultivation
Step 1 selects day fragrant osmanthus, cinnabar osmanthus as cross combination, uses according to long flowering period, the breeding goal of thick flavor The mode of high pressure transplant 20 years or more day fragrant osmanthus, the healthy and strong branch on the veteran of cinnabar osmanthus, cultivate ground as seedling, and predetermined With line-spacing 60-80cm, breadth 30-40cm kinds are planted, at interval of two days sprinkling compound sodium nitrophenolate liquid in one month after transplanting;
Step 2, month next year 4-5 applied nutritional agents, with decomposed thin per 10-15 days by draining and delivery system Based on pig manure liquid, the 6-7 months apply nutritional agents in every 10 days;
Sturdy and well-developed cinnabar osmanthus male flower branch is chosen in step 3, the acquisition and culture of spray, is packed into nutrient solution water It trains in tank, temperature control ensures that cinnabar osmanthus male flower branch is bloomed earlier than day fragrant osmanthus, and cinnabar osmanthus male flower branch Post flowering is entered brosse with writing brush In glassware, it is subsequently placed in refrigerator cold-storage;
Step 4, pollination, the female chapiter of same day fragrant osmanthus is shinny, and when secreting hyalomucoid, male flower pollen is put into female style On in head, interval 10h pollinations are primary, pollinate 3-4 times, and cover with permeable plastics bag;
Step 5, extracts part infructescence, and anti-falling stop seed is stored after ripe according to combination respectively.
Further, 1) step 2 in the soil pre-modified, cycle-index are 4-6 times.
Further, 1) step 2 in the soil pre-modified, the optimization formula weight ratio of soil improvement fertilizer:Pig manure 80-95 parts by weight, activated carbon 5-15 parts by weight, humic acid 25-40 parts by weight, furfural dregs 15-25 parts by weight, composite fertilizer 8-10 Parts by weight, water-retaining agent 1-1.2 parts by weight.
Further, 1) step 2 in the soil pre-modified, the weight ratio of microbial inoculum optimization formula:Nitrogen-fixing bacteria 20-30 Parts by weight, phosphate solubilizing bacteria 15-25 parts by weight, potassium solubilizing bacteria 10-20 parts by weight, antibiotic bacteria 5-8 parts by weight, zymophyte 15-30 parts by weight.
Further, in the step 2, seepage pipe is pvc pipe, embedment subterranean depth between 40cm-120cm, hidden pipe with ooze Pipe is connected to and hidden pipe is connected to bright ditch, and seepage pipe, which is cut, draws underground water to hidden pipe, and Ming Gouzhong, controlling groundwater level are drained into through hidden pipe concentration In critical depth hereinafter, simultaneously nutritional agents directly can be applied to root soil by hidden pipe.
Further, 2) the cross combination selection and step 2, nutritional agents optimization formula ratio in cultivation:80% is decomposed dilute Thin pig manure liquid, 0.5% calcium superphosphate, 0.3% sodium vanadate, 19.2% composite fertilizer.
Beneficial effects of the present invention:
1, it is built by draining and delivery system, can effectively shut off underground water, prevent original underground water from interfering osmanthus fragrans Growth, and can be by pouring into nutritional agents to hidden pipe, improvement solution etc. improves soil, osmanthus fragrans is made to be detached to subacidity growing location Limitation, RS in Alkali Region etc. can be transplanted to extensively.
2, it is built by draining and delivery system, the environmental factor of osmanthus fragrans growth is controllable, and hybrid experiment is done in reduction It disturbs, experimental result is accurate.
3, it selects day fragrant osmanthus, cinnabar osmanthus as cross combination, is improved using the strong characteristic of the long flowering period of day fragrant osmanthus, resistance Cinnabar osmanthus makes the longer blooming period in cinnabar osmanthus, resistance enhancing.
4, cross breeding method of the invention is reasonable, can effectively improve the quality in cinnabar osmanthus.
Specific implementation mode
With reference to embodiments, the specific implementation mode of the present invention is described in further detail, so that technical solution of the present invention It is more readily understood and grasps.
Sweet osmanthus long flowering period hybrid selection method, includes the following steps:
1) soil pre-modified
Step 1, draining and delivery system structure, deep ploughing is predetermined first cultivates 20-40 centimeters or so of ground, preferably deep ploughs It is 30 centimeters, smooth, it is fine crushing with soil, subject to apparent bulk;Then building for canopy constituency is carried out, canopy constituency uses semicircle plastics Booth structure, preferably canopy constituency can add temperature control device, convenient for changing the florescence of osmanthus fragrans, the digging of bright ditch by the variation of temperature Pick is specifically connect in canopy constituency both sides of the edge Wa Qu, and by tools such as water pumps with collector pipe, convenient for draining, seepage pipe, The laying of hidden pipe and collector pipe, specifically, i.e., echelon is embedded at predetermined cultivation ground depth 40cm, at 80cm and at 1.2m oozes Pipe, the moisture in casting earth, it is preferable that the high molecular material that band dredges hole can be used in seepage pipe periphery filtering layer, filtering layer, prevent soil into Entering, angled tilt of seepage pipe is buried, and convenient for draining, hidden pipe is connected in side seepage pipe end, and collector pipe is buried in hidden pipe bottom, For controlling groundwater level and supply nutrition, can by seepage pipe, hidden pipe, collector pipe it is embedded, on the one hand shut off underground water, prevents Underground water hinders the growth of osmanthus fragrans, on the other hand, the growth of osmanthus fragrans can also be controlled by pouring into solution etc., is avoided as possible Composition influence osmanthus fragrans are grown in soil;
Step 2, it is sub- using soil improvement fertilizer, including pig manure, activated carbon, humic acid, furfural dregs, calcium dihydrogen phosphate, sulfuric acid Iron, water-retaining agent and composite fertilizer, rotary tillage after applying every time, spray microbial inoculum, including nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, antibiotic bacteria, hair Yeast-like fungi etc. recycles 4-6 times, according to the optimization formula weight ratio of soil improvement fertilizer:Pig manure 80-95 parts by weight, activated carbon 5- 15 parts by weight, humic acid 25-40 parts by weight, furfural dregs 15-25 parts by weight, composite fertilizer's 8-10 parts by weight, water-retaining agent 1-1.2 weight Part, apply according to 30 kgs/acre, applies the weight ratio of microbial inoculum optimization formula:Nitrogen-fixing bacteria 20-30 parts by weight, phosphate solubilizing bacteria 15-25 Parts by weight, potassium solubilizing bacteria 10-20 parts by weight, antibiotic bacteria 5-8 parts by weight, zymophyte 15-30 parts by weight, spray according to 50g/ mus;
2) cross combination selection and cultivation
Step 1 selects day fragrant osmanthus, cinnabar osmanthus as hybridization according to the strong breeding goal of long flowering period, thick flavor, resistance Combination, transplanted by the way of high pressure 20 years or more day fragrant osmanthus, the healthy and strong branch on the veteran of cinnabar osmanthus, as seedling, specifically For it is preferred choose healthy and strong branch, by branch from bifurcation 10-12 centimeters ring partial applications, then 3 centimeters ring above it Then partial application is peeled the cortex between bipassage ring secant off with knife completely, expose xylem, compost is first prepared before wrapping: Sandy soil or fertile vegetable garden soil (no perennial root class weeds), wood sawdust, moss are mixed in 1: 0.5: 0.5 ratio, never admix chemical fertilizer Or not decomposed organic fertilizer, humidity pinches agglomerating, slightly existing wet print with hand, while getting long 40-50 centimetres, 33-40 centimetres of width ready Plastic film (50-60 plants can be pressed per 1kg films) and plastic tape, most handy 50% methyl α-naphthyl acetate, 2000 times of liquid mix loess and be tuned into Paste is applied to girding wound, and to induce adventitious root, film is first surrounded 4 centimeters of lower section of girding by when wrapping, is used Film lower end bundle is scheduled on branch by plastic tape, then compost is pinched into the mass of soil of 1kg weights, and it is (upper supreme to be wrapped in entire notch 3 centimetres of girdling line), then film reversion is covered into mass of soil, film and mass of soil are bundled into a diameter of 10 centimetres olivary with hand Soil ball, then tighten film in close proximity to soil ball upper end, film connector must be overlapped, to prevent mass of soil moisture evaporation, 15-20 after press strip It is checked that wound goes rotten if finding, should change pressure in time, when seeing a plurality of adventitious root from film external energy, you can will press Seedling is planted from tree, and it is predetermined cultivate with line-spacing 60-80cm, breadth 30-40cm kinds are planted, every in one month after transplanting Compound sodium nitrophenolate liquid was sprayed every two days, promotes root system development;
Step 2, because high pressure is transplanted, sweet osmanthus seedling can pass through draining and delivery system per 10-15 days in month next year 4-5 Apply nutritional agents, nutritional agents optimization formula ratio:80% decomposed thin pig manure liquid, 0.5% calcium superphosphate, 0.3% positive vanadic acid Sodium, 19.2% composite fertilizer, the 6-7 months apply nutritional agents in every 10 days, supplement the nutrients;
Sturdy and well-developed cinnabar osmanthus male flower branch is chosen in step 3, the acquisition and culture of spray, is packed into nutrient solution water It trains in tank, temperature control ensures that cinnabar osmanthus male flower branch is bloomed earlier than day fragrant osmanthus, and temperature is controlled at 16 ° to 18 °, and cinnabar osmanthus male flower branch is opened Brosse is entered in glassware with writing brush, is subsequently placed in refrigerator cold-storage by Hua Hou;
Step 4, pollination, the female chapiter of same day fragrant osmanthus is shinny, and when secreting hyalomucoid, male flower pollen is put into female style On in head, interval 10h pollinations are primary, pollinate 3-4 times, and cover with permeable plastics bag, prevent other pollen from entering, influence to test Result;
Step 5, extracts part infructescence, and anti-falling stop seed is stored after ripe according to combination respectively.
Embodiment 1
In the present embodiment, carry out first, 1) soil pre-modified
Predetermined 30 centimeters or so of the cultivation ground of step 1, draining and delivery system structure, first deep ploughing, it is smooth, it is thin with soil It is broken, subject to apparent bulk;Then building for canopy constituency is carried out, canopy constituency uses semicircle plastics booth structure, installed in canopy constituency Air-conditioning, adjust temperature, the excavation of bright ditch, i.e., in canopy constituency both sides of the edge Wa Qu, canal depth 1.5m, and by water pump tool with catchment Pipe connects, in predetermined cultivation the embedded seepage pipe of echelon at depth 40cm, at 80cm and at 1.2m, the moisture in casting earth, outside seepage pipe Filtering layer is enclosed, filtering layer uses band to dredge the polyurethane material in hole, prevents soil from entering, and seepage pipe is embedded integral into 0.5 ° of inclination angle, is convenient for Draining, hidden pipe are connected in side seepage pipe end, and collector pipe is buried in hidden pipe bottom, for controlling groundwater level and supply nutrition, secretly Pipe and collector pipe are managed using pvc;
Step 2, it is sub- using soil improvement fertilizer, including pig manure, activated carbon, humic acid, furfural dregs, calcium dihydrogen phosphate, sulfuric acid Iron, water-retaining agent and composite fertilizer, rotary tillage after applying every time, spray microbial inoculum, including nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, antibiotic bacteria, hair Yeast-like fungi etc. recycles 5 times, formulation weight part ratio of soil improvement fertilizer:80 parts by weight of pig manure, 5 parts by weight of activated carbon, humic acid 30 Parts by weight, 15 parts by weight of furfural dregs, 9 parts by weight of composite fertilizer, 1 parts by weight of water-retaining agent apply according to 30 kgs/acre, apply microbial inoculum and match The weight ratio of side:25 parts by weight of nitrogen-fixing bacteria, 20 parts by weight of phosphate solubilizing bacteria, 15 parts by weight of potassium solubilizing bacteria, 8 parts by weight of antibiotic bacteria, hair 30 parts by weight of yeast-like fungi, spray according to 50g/ mus;
2) cross combination selection and cultivation
Step 1 selects day fragrant osmanthus, cinnabar osmanthus as hybridization according to the strong breeding goal of long flowering period, thick flavor, resistance Combination, transplanted by the way of high pressure 22 years age of trees day fragrant osmanthus, the healthy and strong branch on the veteran of cinnabar osmanthus chooses as seedling Then healthy and strong branch is used by branch from bifurcation 10-12 centimeters ring partial applications, then 3 centimeters ring partial application above it Knife peels the cortex between bipassage ring secant off completely, exposes xylem, compost is first prepared before wrapping:Sandy soil or fertile vegetable garden Native (no perennial root class weeds), wood sawdust, moss never admix chemical fertilizer or not decomposed organic by 1: 0.5: 0.5 ratio mixing Fertilizer, humidity pinches agglomerating, slightly existing wet print with hand, while getting 45 centimetres long, wide 35 centimetres of plastic film ready (per 1kg films 55 plants can be pressed) and plastic tape, and with 50% methyl α-naphthyl acetate, 2000 times of liquid mix loess and be tuned into paste and be applied to girding wound, to induce Film is first surrounded 4 centimeters of lower section of girding when wrapping by adventitious root, and film lower end bundle is scheduled on branch with plastic tape On, then compost is pinched into the mass of soil of 1kg weights, be wrapped in entire notch (upper 3 centimetres of supreme girdling line), then film is inverted and is covered Film and mass of soil are bundled into a diameter of 10 centimetres of olivary soil ball with hand by firmly mass of soil, then are tightened in close proximity to soil ball upper end thin Film, film connector must be overlapped, and to prevent mass of soil moisture evaporation, be checked within 16 days after press strip, and wound is without going rotten, when from film When external energy sees a plurality of adventitious root, press strip seedling is planted from tree, and in predetermined cultivation with line-spacing 70cm, breadth 35cm kinds It plants, at interval of two days sprinkling 400 times of liquid of compound sodium nitrophenolate in one month after transplanting, promotes root system development;
Step 2, because high pressure is transplanted, sweet osmanthus seedling is applied month next year 4-5, every 10 days by draining and delivery system Nutritional agents, according to 5kg/ mus, nutritional agents formula rate:80% decomposed thin pig manure liquid, 0.5% calcium superphosphate, 0.3% positive vanadium Sour sodium, 19.2% composite fertilizer, the 6-7 months apply nutritional agents in every 10 days, by 3kg/ mus, supplement the nutrients;
Step 3, the acquisition and culture of spray are chosen sturdy and well-developed cinnabar osmanthus male flower branch, bud all risk insurance and are stayed, It is fitted into suspension culture tank, 20 DEG C of room temperature temperature control, ensures that cinnabar osmanthus male flower branch is bloomed earlier than day fragrant osmanthus, 16 DEG C of temperature control in canopy, Fragrant osmanthus bloomed and was later than cinnabar osmanthus day, and brosse is entered in glassware with writing brush, is subsequently placed in ice by cinnabar osmanthus male flower branch Post flowering Case refrigerates;
Step 4, pollination, the female chapiter of same day fragrant osmanthus is shinny, and when secreting hyalomucoid, male flower pollen is put into female style On in head, interval 10h pollinations are primary, pollinate 3 times, and cover with permeable plastics bag, prevent other pollen from entering, and influence experiment As a result;
Step 5 extracts part infructescence, is extractd according to 10% ratio, anti-falling stop seed, is stored respectively according to combination after ripe.
The seed of the method output of the present invention 2009, carries out outdoor on Suzhou, Zhengzhou, three ground of Beijing and pilots kind respectively, It blooms after plantation in 6 years, wherein 2016, Suzhou District new varieties flowering time is August 16, and Zhengzhou area is August 18 Day, Beijing area is August 20, and specifically following data was obtained by 2017:
Table one
Survival rate Incidence Infested rate Plant growing way
Suzhou 88% 6.2% 7.1% Flourishing, root is more, and blade face is plump
Zhengzhou 86% 8.6% 7.8% Flourishing, root is more, and blade face is plump
Beijing 84% 8.9% 7.9% There is seldom weak branch
Table two
Florescence/day It blooms the time limit Flowering time
Suzhou 22 6 15
Zhengzhou 20 6 13
Beijing 19 6 12
Note:Data are 2016/2017 year continuous 2 years mean value in table two;
Flowering time:2016/2017 year, compared to the number of days mean value shifted to an earlier date with ground cinnabar osmanthus flowering time;
By table one it is known that three weather differently band, the new varieties osmanthus fragrans are hybridized through this selection It grows fine, survival rate is higher than 80%, and incidence, infested rate gradually rise with climate cooling, but is less than 10%, plant growing way Good, in Beijing area, there are the weak branches in part, and for traditional cinnabar osmanthus, this selection hybridizes the new varieties sweet osmanthus Resistance enhancing is set, the enhancing of severe cold ability is adapted to.
By table two it is known that blooming within equal 6 years three, traditional cinnabar osmanthus seeding method needs 10 years or more just bloom, This selection hybridizes the time limit shortening of blooming of the new varieties, and traditional cinnabar sweet osmanthus phase at 15 days or so, this selection and breeding Method hybridize the new varieties the minimum florescence be 19 days, in addition traditional cinnabar osmanthus flowering time is in September-October, this selection and breeding side Method hybridizes the new varieties cinnabar osmanthus and at least shifts to an earlier date opening in 12 days, and aromatic flavour than traditional cinnabar osmanthus.
Certainly, the specific application example that the above is only the present invention, is not limited in any way protection scope of the present invention.It is all The technical solution formed using equivalent transformation or equivalent replacement is all fallen within rights protection scope of the present invention.

Claims (6)

1. sweet osmanthus long flowering period hybrid selection method, which is characterized in that include the following steps:
1) soil pre-modified
Step 1, draining and delivery system structure, deep ploughing is predetermined first cultivates 20-40 centimeters of ground, smooth, nothing fine crushing with soil Subject to apparent bulk;Then it carries out canopy constituency to build, the excavation of bright ditch, the laying of seepage pipe, hidden pipe and collector pipe, it is bright ditch, dark Pipe and collector pipe are connected to, for controlling groundwater level and supply nutrition;
Step 2, using soil improvement fertilizer, including pig manure, activated carbon, humic acid, furfural dregs, calcium dihydrogen phosphate, ferrous sulfate, Water-retaining agent and composite fertilizer, rotary tillage after applying every time, spray microbial inoculum, including nitrogen-fixing bacteria, phosphate solubilizing bacteria, potassium solubilizing bacteria, antibiotic bacteria, zymophyte Deng cycle is multiple;
2) cross combination selection and cultivation
Step 1 selects day fragrant osmanthus, cinnabar osmanthus as hybridization group according to the strong breeding goal of long flowering period, thick flavor, resistance Close, transplanted by the way of high pressure 20 years or more day fragrant osmanthus, the healthy and strong branch on the veteran of cinnabar osmanthus, as seedling, and pre- With line-spacing 60-80cm, breadth 30-40cm kinds are planted on fixed ground of cultivating, at interval of two days sprinkling compound sodium nitrophenolates in one month after transplanting Liquid;
Step 2, month next year 4-5 applied nutritional agents, with decomposed thin pig manure per 10-15 days by draining and delivery system Based on liquid, the 6-7 months apply nutritional agents in every 10 days;
Sturdy and well-developed cinnabar osmanthus male flower branch is chosen in step 3, the acquisition and culture of spray, is packed into suspension culture tank In, temperature control ensures that cinnabar osmanthus male flower branch is bloomed earlier than day fragrant osmanthus, and brosse is entered glass by cinnabar osmanthus male flower branch Post flowering with writing brush In vessel, it is subsequently placed in refrigerator cold-storage;
Step 4, pollination, the female chapiter of same day fragrant osmanthus is shinny, and when secreting hyalomucoid, cinnabar osmanthus male flower pollen is put into female flower On in column cap, interval 10h pollinations are primary, pollinate 3-4 times, and cover with breathable polybag;
Step 5, extracts part infructescence, and anti-falling stop seed is stored after ripe according to combination respectively.
2. sweet osmanthus long flowering period hybrid selection method according to claim 1, which is characterized in that 1) in the soil pre-modified Step 2, cycle-index are 4-6 times.
3. sweet osmanthus long flowering period hybrid selection method according to claim 1, which is characterized in that 1) in the soil pre-modified Step 2, the optimization formula weight ratio of soil improvement fertilizer:Pig manure 80-95 parts by weight, activated carbon 5-15 parts by weight, humic acid 25-40 parts by weight, furfural dregs 15-25 parts by weight, composite fertilizer's 8-10 parts by weight, water-retaining agent 1-1.2 parts by weight.
4. sweet osmanthus long flowering period hybrid selection method according to claim 1, which is characterized in that 1) in the soil pre-modified Step 2, the weight ratio of microbial inoculum optimization formula:Nitrogen-fixing bacteria 20-30 parts by weight, phosphate solubilizing bacteria 15-25 parts by weight, potassium solubilizing bacteria 10- 20 parts by weight, antibiotic bacteria 5-8 parts by weight, zymophyte 15-30 parts by weight.
5. sweet osmanthus long flowering period hybrid selection method according to claim 1, which is characterized in that 1) in the soil pre-modified Step 1, seepage pipe are pvc pipe, and embedment subterranean depth is between 40cm-120cm, and hidden pipe is connected to seepage pipe and hidden pipe connects with bright ditch Logical, seepage pipe, which is cut, draws underground water to hidden pipe, drains into Ming Gouzhong through hidden pipe concentration, controlling groundwater level can lead in critical depth It crosses hidden pipe and directly applies nutritional agents to root soil.
6. sweet osmanthus long flowering period hybrid selection method according to claim 1, which is characterized in that 2) the cross combination selection With step 2 in cultivation, nutritional agents optimization formula ratio:80% decomposed thin pig manure liquid, 0.5% calcium superphosphate, 0.3% are just Sodium vanadate, 19.2% composite fertilizer.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102242543A (en) * 2010-05-11 2011-11-16 上海市农业科学院 Water conservancy system for preventing and treating soil secondary salinization in greenhouses in coastal region
CN102674996A (en) * 2012-05-23 2012-09-19 石光忠 Microbial organic fertilizer and manufacture method of microbial organic fertilizer
CN102805030A (en) * 2012-08-03 2012-12-05 侯金波 Crossbreeding technique of bamboo willow
CN104365304A (en) * 2013-08-12 2015-02-25 天津市增益花卉苗木销售有限公司 Cutting propagation method of Osmanthus fragrans
CN106465665A (en) * 2015-08-18 2017-03-01 华蓥市榆桂种植专业合作社 A kind of scientific and effective Osmanthus tragranscv.Aurantiacu implantation methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102242543A (en) * 2010-05-11 2011-11-16 上海市农业科学院 Water conservancy system for preventing and treating soil secondary salinization in greenhouses in coastal region
CN102674996A (en) * 2012-05-23 2012-09-19 石光忠 Microbial organic fertilizer and manufacture method of microbial organic fertilizer
CN102805030A (en) * 2012-08-03 2012-12-05 侯金波 Crossbreeding technique of bamboo willow
CN104365304A (en) * 2013-08-12 2015-02-25 天津市增益花卉苗木销售有限公司 Cutting propagation method of Osmanthus fragrans
CN106465665A (en) * 2015-08-18 2017-03-01 华蓥市榆桂种植专业合作社 A kind of scientific and effective Osmanthus tragranscv.Aurantiacu implantation methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
李稚: "桂花传粉生物学与杂交育种研究", 《中国优秀硕士学位论文全文数据库(电子期刊)-农业科技辑》 *
沈植国等: "《焦桐高速公路泌阳段景观绿化与模式配置研究》", 31 August 2013, 黄河水利出版社 *

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