CN108431015A - 一种nadph的纯化工艺 - Google Patents
一种nadph的纯化工艺 Download PDFInfo
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- CN108431015A CN108431015A CN201680016191.8A CN201680016191A CN108431015A CN 108431015 A CN108431015 A CN 108431015A CN 201680016191 A CN201680016191 A CN 201680016191A CN 108431015 A CN108431015 A CN 108431015A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/027—Nanofiltration
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/147—Microfiltration
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
- C07H19/207—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids the phosphoric or polyphosphoric acids being esterified by a further hydroxylic compound, e.g. flavine adenine dinucleotide or nicotinamide-adenine dinucleotide
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
Description
Claims (1)
- 权利要求书[权利要求 1] 一种 NADPH的纯化工艺, 其特征在于: 所述工艺依次包括以下步骤A、 预处理: 将生物催化法制备得到的 NADPH粗产物过微滤膜, 所 述微滤膜为 0.1-0.5μηι孔径的中空纤维膜。Β、 上离子柱: 将步骤 Α微滤后的 NADPH粗产物上阴离子交换树脂柱C、 阳离子洗脱: 上样完成后用纯水淋洗离子柱至洗脱液中不含阳离 子;D、 前杂洗脱: 用 0.07-0.5mol/L氯化钠溶液淋洗离子柱至洗脱液中不 含在 260nm波长处有紫外吸收的物质;E、 产品洗脱: 用含有 0.5mol/L氯化钠、 Ί% (V/V%) 乙醇的水溶液 淋洗离子柱, 收集在 340nm波长处有紫外吸收的洗脱液;F、 步骤 E的洗脱液经浓缩、 干燥处理后即得纯化后的 NADPH成品。[权利要求 2] 根据权利要求 1所述的 NADPH的纯化工艺, 其特征在于: 所述阴离 子交换树脂为大孔型苯乙烯系季胺 I型强碱性阴离子交换树脂。[权利要求 3] 根据权利要求 2所述的 NADPH的纯化工艺, 其特征在于: 所述 NADPH粗产物的上样量以 NADPH计为 9-15g/g阴离子交换树脂。[权利要求 4] 根据权利要求 1或 2所述的 NADPH的纯化工艺, 其特征在于, 当所述NADPH粗产物的 pH值较低吋, 所述工艺还包括: 在步骤 A之前, 将 所述 NADPH粗产物的 pH值调至 8.0-11.0。[权利要求 5] 根据权利要求 4所述的 NADPH的纯化工艺, 其特征在于, 当所述 NADPH粗产物的 pH值较低吋, 所述工艺还包括: 在步骤 A之前, 将所述NADPH粗产物的 pH值调至 9.0-10.0。[权利要求 6] 根据权利要求 1或 2所述的 NADPH的纯化工艺, 其特征在于, 所述工 艺还包括对所述阴离子交换树脂的如下预处理步骤: 用氢氧化钠溶液 浸泡所述阴离子交换树脂, 然后用纯水淋洗至偏中性。[权利要求 7] 根据权利要求 1或 2所述的 NADPH的纯化工艺, 其特征在于: 所述工 艺还包括将所述阴离子交换树脂再生的步骤, 再生过程中所使用的再 生液为含有 1.0mol/L氯化钠、 O.lmol/L盐酸的水溶液, 淋洗速度为 0.8- 1.5BV/H, 再生液用量为 2.0BV, 再生后用纯水冲洗树脂至偏中性。[权利要求 8] 根据权利要求 1或 2所述的 NADPH的纯化工艺, 其特征在于: 步骤 C 和步骤 D中全程采用核酸蛋白检测仪进行检测, 所述洗脱液的收集方 式为从所述核酸蛋白检测仪的读数幵始上升吋幵始收集, 待读数幵始 下降吋停止收集。[权利要求 9] 根据权利要求 1或 2所述的 NADPH的纯化工艺, 其特征在于: 所述浓 缩处理依次包括微滤、 超滤和纳滤, 所述微滤处理使用 0.1-0.5μηι孔 径的中空纤维膜, 所述超滤处理使用截留分子量为 10K的超滤膜, 所 述纳滤处理使用截留分子量为 100-400的卷式膜。[权利要求 10] 根据权利要求 1或 2所述的 NADPH的纯化工艺, 其特征在于: 所述干 燥处理为真空冷冻干燥。
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PCT/CN2016/109849 WO2018107377A1 (zh) | 2016-12-14 | 2016-12-14 | 一种nadph的纯化工艺 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112870979A (zh) * | 2020-12-31 | 2021-06-01 | 内蒙古金达威药业有限公司 | 一种特种膜及其制备方法和应用以及β-烟酰胺单核苷酸的分离纯化方法 |
CN113354702A (zh) * | 2021-06-03 | 2021-09-07 | 杭州唯泰生物药业有限公司 | 一种高纯nadp的分离提纯工艺 |
CN114544787A (zh) * | 2020-11-25 | 2022-05-27 | 尚科生物医药(上海)有限公司 | 一种nadp中有关物质的检测方法 |
CN115353537A (zh) * | 2022-07-29 | 2022-11-18 | 深圳希吉亚生物技术有限公司 | 一种nmnh的纯化工艺 |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112778358A (zh) * | 2019-11-08 | 2021-05-11 | 中国科学院天津工业生物技术研究所 | 一种体外多酶体系制备的小分子化合物的分离提取方法 |
JP7313296B2 (ja) | 2020-01-31 | 2023-07-24 | イビデン株式会社 | マット材 |
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CN104892710A (zh) * | 2015-05-19 | 2015-09-09 | 邦泰生物工程(深圳)有限公司 | 一种纯化还原型β-烟酰胺腺嘌呤二核苷酸的方法 |
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CN105463043A (zh) * | 2016-01-06 | 2016-04-06 | 北京化工大学 | 一种酶法再生atp的方法 |
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- 2016-12-14 US US15/575,389 patent/US10765967B2/en active Active
- 2016-12-14 JP JP2017559118A patent/JP6510074B2/ja active Active
- 2016-12-14 CN CN201680016191.8A patent/CN108431015B/zh active Active
- 2016-12-14 WO PCT/CN2016/109849 patent/WO2018107377A1/zh active Application Filing
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US3749709A (en) * | 1968-03-20 | 1973-07-31 | Boehringer Mannheim Gmbh | Process for the purification of reduced pyridine coenzymes |
JPS49100292A (zh) * | 1973-01-30 | 1974-09-21 | ||
RO96425B1 (ro) * | 1982-04-24 | 1989-03-02 | Institutul De Chimie Macromoleculara "Petru Poni" | Procedeu de preparare a unor schimbatori de anioni |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114544787A (zh) * | 2020-11-25 | 2022-05-27 | 尚科生物医药(上海)有限公司 | 一种nadp中有关物质的检测方法 |
CN112870979A (zh) * | 2020-12-31 | 2021-06-01 | 内蒙古金达威药业有限公司 | 一种特种膜及其制备方法和应用以及β-烟酰胺单核苷酸的分离纯化方法 |
CN112870979B (zh) * | 2020-12-31 | 2022-12-23 | 内蒙古金达威药业有限公司 | 一种β-烟酰胺单核苷酸的分离纯化方法 |
CN113354702A (zh) * | 2021-06-03 | 2021-09-07 | 杭州唯泰生物药业有限公司 | 一种高纯nadp的分离提纯工艺 |
CN115353537A (zh) * | 2022-07-29 | 2022-11-18 | 深圳希吉亚生物技术有限公司 | 一种nmnh的纯化工艺 |
Also Published As
Publication number | Publication date |
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US20190336887A1 (en) | 2019-11-07 |
JP6510074B2 (ja) | 2019-05-08 |
JP2019503334A (ja) | 2019-02-07 |
WO2018107377A1 (zh) | 2018-06-21 |
CN108431015B (zh) | 2021-03-23 |
US10765967B2 (en) | 2020-09-08 |
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