CN108424208A - A kind of mill water culture nutrient solution and its preparation and application suitable for soybean nodulation - Google Patents
A kind of mill water culture nutrient solution and its preparation and application suitable for soybean nodulation Download PDFInfo
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- CN108424208A CN108424208A CN201810570750.0A CN201810570750A CN108424208A CN 108424208 A CN108424208 A CN 108424208A CN 201810570750 A CN201810570750 A CN 201810570750A CN 108424208 A CN108424208 A CN 108424208A
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- soybean
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
- Hydroponics (AREA)
Abstract
The present invention relates to a kind of mill water culture nutrient solutions and its preparation and application suitable for soybean nodulation, are made of potassium nitrate, calcium nitrate, calcium chloride, ammonium nitrate, potassium sulfate, magnesium sulfate, magnesium chloride, manganese sulfate, ammonium sulfate, zinc sulfate, copper sulphate, ammonium molybdate, Boratex, cobalt chloride, EDTA molysite and potassium dihydrogen phosphate.The mill water culture nutrient solution of the present invention can dramatically increase the development of the root nodule numbers, nodule weight, promotion root nodule bacteroid of soybean, can finally soybean be promoted to grow, increase soybean root system and overground part dry weight compared with 1/2 Hoagland nutrient solutions.The mill water culture nutrient solution of the present invention, there is some superiority including the development of the organ growths such as root system and root nodule and the experiment for carrying out relevant physiological and molecule mechanism using soybean hydroponic culture systems Study of The Underground portion, the legume especially including soybean provides the culture formula that can refer to and method condition in the research of Plant Nutrition, root system development and nodulation and nitrogen fixation effect etc..
Description
Technical field
The present invention relates to a kind of its preparation and application of the mill water culture nutrient solution suitable for soybean nodulation, belong to water planting nutrition
Liquid technical field.
Background technology
Soybean is not only protein and oil source important in human food's structure, and is that typical biological nitrogen fixation is made
Object.The study found that the symbiotic nitrogen fixation that legume-rhizobium are formed accounts for about 60% or more of biological nitrogen fixation total amount, it is nitrogen-fixing efficiency
The most system of highest, amount of nitrogen fixation（Herridge, et al., 2008）.It is raw that soybean biological nitrogen fixation is applied to agricultural
Production can improve crop nitrogen nutrition, reduce nitrogenous fertilizer input, promote root growth and improve crop yield（Ferreira et
al., 2009）.In the soybean main production country such as the U.S., Brazil, Rhizobium Inoculation has become one of major measure of soybean yield-increasing.And
Legume inoculation technology is applied to agricultural production by China, also achieves good benefit（Tang Fuyue etc. 2011; Mendes et
al., 2003; Sogut 2006; de Freitas et al., 2012）.As it can be seen that the physiology of research soybean nodulation fixed nitrogen is made
With with molecular regulation mechanism, excavate soybean biological fixed nitrogen potentiality to improve crop nutrition content efficiency, and push the ecological agriculture can
Sustainable development etc. has far reaching significance.
Soybean mill water culture nutrient solution culture convenient for control supply nutrient content, observation underground part phenotype and obtains underground part sample
Product are the important method systems of bean plant nutrition, root system biology and nodulation and nitrogen fixation research.Studies have shown that nutrient solution at
Point and content number seriously affected soybean nodulation fixed nitrogen.In general, low nitrogen processing, can promote soybean nodulation, increase root
Tumor number；And high nitrogen processing, significantly suppress soybean nodulation and bacteroid development（Fujikake et al., 2003）.Have
1/2 Hoagland mill water culture nutrient solution cultures soybean nodulation number it is relatively fewer, not soybean growth and dross be most
Good nutrient formula.Therefore, it is necessary to make the obligate mill water culture nutrient solution suitable for soybean nodulation, and then it is soybean nodulation fixed nitrogen
The basic research of physiology and molecule provides cultural method support and reference frame.
Invention content
Based on the studies above background, problem to be solved by this invention is to provide a kind of suitable soybean nodulation, plant life
Long healthy and strong mill water culture nutrient solution.
In order to solve the above technical problems, the present invention provides a kind of mill water culture nutrient solution suitable for soybean nodulation, key component
Including：0.15 mM of potassium nitrate, 0.12 mM of calcium nitrate, 1.2 mM of calcium chloride, 0.04 mM of ammonium nitrate, 0.3 mM of potassium sulfate, sulfuric acid
0.5 mM of magnesium, 0.025 mM of magnesium chloride, 0.0015 mM of manganese sulfate, 0.03 mM of ammonium sulfate, 0.0015 mM of zinc sulfate, copper sulphate
0.0005 mM, 0.00015 mM of ammonium molybdate, 0.0025 mM of Boratex, 0.0001 mM, EDTA- molysite of cobalt chloride, 0.04 mM and
0.25 mM of potassium dihydrogen phosphate.Wherein, the nutrient solution is by following 8 mother liquors（stock）It configures：Stock1 includes nitric acid
Potassium, calcium nitrate and ammonium nitrate；Stock2 includes ammonium sulfate；Stock3 includes magnesium sulfate, magnesium chloride, manganese sulfate, zinc sulfate, sulfuric acid
Copper, ammonium molybdate and cobalt chloride；Stock4 includes Boratex；Stock5 includes EDTA- molysite；Stock6 includes potassium dihydrogen phosphate；
Stock7 includes potassium sulfate；Stock8 includes calcium chloride.In the nutrient solution of Rhizobium Inoculation experiment, the nitrogen concentration of nutrient solution is
0.53 mM, potassium are supplemented with stock7 potassium sulfates.
A kind of preparation method of mill water culture nutrient solution suitable for soybean nodulation includes as described above：By stock1-
Stock8 dilutes 1000 times, takes stock1-stock8 to dilute the liquid mixing after 1000 times, obtains nutrient solution.
A kind of application method of mill water culture nutrient solution suitable for soybean nodulation：When using Solution culture method soybean, including with
Lower step：
A, the nutrient solution configured, with 0.5 mM H2SO4Or 1 mM KOH pH is adjusted to 5.7-5.9 after, packing is to incubator；
B, will 7 days soybean seedling Rhizobium Inoculations of growth, and migrate in the nutrient solution after adjusting pH, ventilate 10 per hour
Min adjusts a pH, pH is made to maintain 5.7-5.9 every three days；When growing 14 days, ensureing former 0.3 mM potassium sulfate demands
On the basis of, it is supplemented potassium sulfate, makes to contain 0.45 mM potassium sulfates in nutrient solution；When growing 21 days, it is supplemented potassium sulfate, makes nutrition
Contain 0.6 mM potassium sulfate amounts in liquid；When growing 28 days, it is supplemented potassium sulfate, makes to contain 0.9 mM potassium sulfates in nutrient solution.
The invention has the beneficial effects that：By compared with existing 1/2 Hoagland nutrient solutions, being carried using the present invention
The soybean mill water culture nutrient solution of confession（Nitrogen concentration total amount is 0.53 mM）, soybean nodulation number, root nodule dry weight can be dramatically increased and promoted
Root nodule bacteroid is developed, and then promotes soybean growth, increases its overground part and weight of root system.Therefore, utilize the present invention's
The soybean plant strain root nodule of soybean Nourishing Formulations for Water Cultivation, culture is more and plant strain growth is healthy and strong, suitable for carrying out and planting using this formula
The correlation tests such as object nutrition, Root Symbiont.Meanwhile using this mill water culture nutrient solution system convenient for observation underground part organ, as root system,
Root nodule etc. develops phenotype, consolidates nitrogen studies for soybean nodulation and provides Solution culture method scheme and method.
Description of the drawings
Fig. 1 be Different Nutrition liquid under the conditions of soybean nodulation grow to relatively analyze；
Fig. 2 is that Different Nutrition liquid is handled to soybean nodulation development and the influence of nitrogenase activity；
Fig. 3 is the comparative analysis of soybean growth under the conditions of Different Nutrition liquid.
Specific implementation mode
Specific introduce is made to the present invention below in conjunction with the drawings and specific embodiments.
Embodiment 1：Configuration suitable for soybean nodulation mill water culture nutrient solution
A kind of mill water culture nutrient solution suitable for soybean nodulation provided by the invention, key component include：0.15 mM of potassium nitrate, nitre
0.12 mM of sour calcium, 1.2 mM of calcium chloride, 0.04 mM of ammonium nitrate, 0.3 mM of potassium sulfate, 0.5 mM of magnesium sulfate, magnesium chloride 0.025
MM, 0.0015 mM of manganese sulfate, 0.03 mM of ammonium sulfate, 0.0015 mM of zinc sulfate, 0.0005 mM of copper sulphate, ammonium molybdate
0.00015 mM, 0.0025 mM of Boratex, 0.0001 mM, EDTA- molysite of cobalt chloride, 0.04 mM and potassium dihydrogen phosphate 0.5
mM.The nutrient solution is by following 8 mother liquors（stock）It configures：Stock1 includes potassium nitrate, calcium nitrate and ammonium nitrate；
Stock2 includes ammonium sulfate；Stock3 includes magnesium sulfate, magnesium chloride, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and cobalt chloride；
Stock4 includes Boratex；Stock5 includes EDTA- molysite；Stock6 includes potassium dihydrogen phosphate；Stock7 includes potassium sulfate；
Stock8 includes calcium chloride.
The mentioned reagent for weighing different quality respectively is dissolved in distilled water, and pre-prepd blue lid bottle is transferred to after constant volume,
Stock liquid is prepared, the concentration of each ingredient is final concentration in stock liquid（Nutrient solution）1000 times.Wherein, stock5 includes
EDTA- molysite needs are kept in dark place, or are stored in brown bottle.Stock1- stock8 are taken to dilute the liquid after 1000 times mixed
It closes, obtains nutrient solution.
Embodiment 2：Under the conditions of Different Nutrition liquid soybean nodulation grow to relatively analyze
Germination 7 days in vermiculite soya seeds HN66, Rhizobium Inoculation be transferred to respectively after 1 hour 1/2 Hoagland and
Soybean nutritional liquid（pH=5.7-5.9）Middle processing.Wherein nitrogen total amount is：0.53 mM （NH4NO3+KNO3+Ca (NO3)2·
4H2O）.Ventilate 10 min per hour, adjusts a pH every three days, pH is made to maintain 5.7-5.9.When soybean grows 14 days, ensureing
On the basis of former 0.3 mM potassium sulfate demands, it is supplemented potassium sulfate, makes to contain 0.45 mM potassium sulfates in nutrient solution；Growth 21 days
When, it is supplemented potassium sulfate, makes to contain 0.6 mM potassium sulfate amounts in nutrient solution；When growing 28 days, it is supplemented potassium sulfate, is made in nutrient solution
Containing 0.9 mM potassium sulfates.This experiment when soybean grows 28 days, harvests the table under simultaneously comparative analysis Different Nutrition liquid condition of culture
Type.
Fig. 1 is the comparative analysis of soybean nodulation growth under the conditions of Different Nutrition liquid.Wherein：
In Fig. 1（A）For Nodule Growth phenotype；
In Fig. 1（B）For root nodule numbers；
In Fig. 1（C）For root nodule dry weight.
Data are the average value and standard error that 4 biology repeat in figure.
Asterisk indicates the significant difference between same character under the conditions of 1/2 Hoagland and soybean nutritional liquid：
* the level of signifiance 0.01 is indicated<When ρ≤0.05, significant difference；
* indicates the level of signifiance 0.001<When ρ≤0.01, the significance of difference between significantly with extremely significantly between；
When * * indicate level of signifiance ρ≤0.001, difference is extremely notable.
The result shows that：
Compared with 1/2 Hoagland nutrient solutions, obviously increased greatly using soybean nutritional liquid energy（The mm of diameter >=2）, it is small（Directly
Diameter< 2 mm）Root nodule number, referring to Fig. 1（A and B）And large and small root nodule dry weight, referring to Fig. 1（B）, imply that soybean nutritional liquid
Suitable for the culture to plant in soybean nodulation correlation test.
Embodiment 3：The processing of Different Nutrition liquid is to soybean nodulation development and the influence of nitrogenase activity
It harvests respectively and grows 28 days root nodule samples under the conditions of 1/2 Hoagland and soybean nutritional liquid, cut by paraffin
The developmental state of piece, Toluidine blue staining observation root nodule bacteroid.Acetylene reduction method is further utilized, the azotase of root nodule is analyzed
Activity.
Fig. 2 is that Different Nutrition liquid is handled to soybean nodulation development and the influence of nitrogenase activity.Wherein：
In Fig. 2（A）For the developmental condition of root nodule bacteroid under the conditions of 1/2 Hoagland nutrient solutions；
In Fig. 2（B）For the developmental condition of root nodule bacteroid under the conditions of soybean nutritional liquid；
In Fig. 2（C）For in Fig. 2（A）Enlarged drawing；
In Fig. 2（D）For in Fig. 2（B）Enlarged drawing；
In Fig. 2（E）For nitrogenase activity.
In Fig. 2（A）、（B）、（C）With（D）Scale be 1000 μm.
Data are the average value and standard error that 4 biology repeat in figure.
The result shows that：
Compared with 1/2 Hoagland nutrient solutions, it is obviously promoted root nodule bacteroid using soybean nutritional liquid energy and develops, referring to Fig. 2
In（C and D）, and then its nitrogenase activity is made to increase trend, referring in Fig. 2（E）.Illustrate, soybean nutritional liquid energy promotes Soybean Root
The growth and development of tumor.
Embodiment 4：The comparative analysis that soybean grows under the conditions of Different Nutrition liquid
It harvests respectively under the conditions of 1/2 Hoagland and soybean nutritional liquid, 28 days soybean plant strains is grown after Rhizobium Inoculation.It receives
The sample obtained finishes 30 minutes in 105 DEG C of baking ovens and is placed on 75 DEG C drying to constant weight, weighs overground part and weight of root system respectively.
Fig. 3 is the comparative analysis of soybean growth under the conditions of Different Nutrition liquid.Wherein：
In Fig. 3（A）For soybean growth phenotype；
In Fig. 3（B）For overground part dry weight；
In Fig. 3（C）For weight of root system.
Data are the average value and standard error that 4 biology repeats in figure.
Asterisk indicates the significant difference between same character under the conditions of 1/2 Hoagland and soybean nutritional liquid：
* the level of signifiance 0.01 is indicated<When ρ≤0.05, significant difference；
* indicates the level of signifiance 0.001<When ρ≤0.01, the significance of difference between significantly with extremely significantly between.
The result shows that：
Compared with 1/2 Hoagland nutrient solutions, the ingredient and proportioning of soybean nutritional liquid can be obviously promoted soybean overground part and root
The growth of system, referring in Fig. 3（A）, soybean overground part and weight of root system are significantly increased, referring in Fig. 3（B and C）.
In conclusion a kind of nutrient solution prescription suitable for soybean nodulation provided by the invention, is utilizing soybean water planting body
Being the organ growth development of Study of The Underground portion and the experiment of development relevant physiological and molecule mechanism has some superiority, especially in water
Rhizobium Inoculation under the conditions of training studies soybean nodulation nitrogen fixation.Compared with 1/2 Hoagland nutrient solutions, sought using soybean
The soybean of nutrient solution culture can obviously increase nodule number, root nodule dry weight, promote the development of root nodule bacteroid and further promote soybean
Growth increases overground part and weight of root system.As it can be seen that a kind of soybean nutritional liquid provided by the invention is in soybean hydroponic system, promote
Into soybean nodulation preferably nutrient solution prescription.
It should be noted that the invention is not limited in any way for above-described embodiment, it is all to use equivalent replacement or equivalent change
The technical solution that the mode changed is obtained, all falls in protection scope of the present invention.
Claims (5)
1. a kind of mill water culture nutrient solution suitable for soybean nodulation, which is characterized in that nutrient composition includes following components：Potassium nitrate
0.15 mM, 0.12 mM of calcium nitrate, 1.2 mM of calcium chloride, 0.04 mM of ammonium nitrate, 0.3 mM of potassium sulfate, 0.5 mM of magnesium sulfate, chlorine
Change 0.025 mM of magnesium, 0.0015 mM of manganese sulfate, 0.03 mM of ammonium sulfate, 0.0015 mM of zinc sulfate, 0.0005 mM of copper sulphate, molybdenum
0.00015 mM of sour ammonium, 0.0025 mM of Boratex, 0.0001 mM, EDTA- molysite of cobalt chloride, 0.04 mM and potassium dihydrogen phosphate
0.25 mM。
2. a kind of mill water culture nutrient solution suitable for soybean nodulation according to claim 1, it is characterised in that：The nutrient solution
It is configured by following 8 stock, wherein stock1 includes potassium nitrate, calcium nitrate and ammonium nitrate；Stock2 includes ammonium sulfate；
Stock3 includes magnesium sulfate, magnesium chloride, manganese sulfate, zinc sulfate, copper sulphate, ammonium molybdate and cobalt chloride；Stock4 includes Boratex；
Stock5 includes EDTA- molysite；Stock6 includes potassium dihydrogen phosphate；Stock7 includes potassium sulfate；Stock8 includes calcium chloride.
3. a kind of mill water culture nutrient solution suitable for soybean nodulation according to claim 1 or 2, it is characterised in that：Nutrient solution
Nitrogen concentration total amount be 0.53 mM, potassium need with stock7 potassium sulfates supplement.
4. a kind of preparation method of mill water culture nutrient solution suitable for soybean nodulation as claimed in claim 2, it is characterised in that：Institute
Stating preparation method includes：Stock1-stock8 is diluted 1000 times, takes stock1-stock8 to dilute the liquid after 1000 times mixed
It closes, obtains nutrient solution.
5. a kind of application method of mill water culture nutrient solution suitable for soybean nodulation as claimed in claim 2, it is characterised in that：Make
When with Solution culture method soybean, include the following steps：
A, the nutrient solution configured, with 0.5 mM H2SO4Or 1 mM KOH pH is adjusted to 5.7-5.9 after, packing is to incubator；
B, will 7 days soybean seedling Rhizobium Inoculations of growth, and migrate in the nutrient solution after adjusting pH, ventilate 10 per hour
Min adjusts a pH, pH is made to maintain 5.7-5.9 every three days；When growing 14 days, ensureing former 0.3 mM potassium sulfate demands
On the basis of, it is supplemented potassium sulfate, makes to contain 0.45 mM potassium sulfates in nutrient solution；When growing 21 days, it is supplemented potassium sulfate, makes nutrition
Contain 0.6 mM potassium sulfate amounts in liquid；When growing 28 days, it is supplemented potassium sulfate, makes to contain 0.9 mM potassium sulfates in nutrient solution.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112931004A (en) * | 2021-02-03 | 2021-06-11 | 浙江省林业科学研究院 | Method for analyzing root nodule formation of different varieties of waxberries |
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| CN1040975A (en) * | 1988-09-09 | 1990-04-04 | 谢宏年 | The leaf fertilizer and the making method of controlling plant diseases |
| CN104744093A (en) * | 2013-12-30 | 2015-07-01 | 刘芳 | Nutrient solution for flowers |
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| 寇建平等: "《优质专用大豆品种及高产栽培技术》", 31 August 2003, 中国农业出版社 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112931004A (en) * | 2021-02-03 | 2021-06-11 | 浙江省林业科学研究院 | Method for analyzing root nodule formation of different varieties of waxberries |
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Application publication date: 20180821 |