CN108401785A - A kind of needle mushroom nutrient special and preparation method thereof - Google Patents
A kind of needle mushroom nutrient special and preparation method thereof Download PDFInfo
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- CN108401785A CN108401785A CN201810327984.2A CN201810327984A CN108401785A CN 108401785 A CN108401785 A CN 108401785A CN 201810327984 A CN201810327984 A CN 201810327984A CN 108401785 A CN108401785 A CN 108401785A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The technical problem to be solved in the present invention is to provide a kind of needle mushroom nutrient special and preparation method thereof, the ingredient and weight proportion of the nutritional agents of the invention are：High 100 150 parts of side calanthe petal, 260 320 parts of pod Opulus leaf stirrups blades, 300 350 parts of parsley Mang ox seedling fruit, 550 parts of water.The present invention combines multiple element, effectively reduces the content of dietary fiber in needle mushroom, while improving the content of vitamin C in needle mushroom, carrotene, protein, zinc, to make up the deficiencies in the prior art.
Description
Technical field
The present invention relates to a kind of nutritional agents, especially a kind of content effectively reducing dietary fiber in needle mushroom, simultaneously
Improve the nutritional agents of the content of vitamin C, carrotene, protein, zinc in needle mushroom.
Background technology
Needle mushroom, Chinese scientific name are hair handle money bacterium, and Classification system is Flammulina velutiper (Fr.) Sing,
There is needle mushroom very high medicinal dietary function, fresh Ion Absorption of Flammulina Velutipes to contain vitamin C, carbohydrate, carrotene, protein etc.,
But also contain more chitin in needle mushroom, although being also classified under dietary fiber, these fibers are different from vegetable
Cellulose in dish, our digestive system are fertilizer that is helpless, and being applied at present to needle mushroom to this substance
Predominantly common chemical fertilizer or organic fertilizer to the content dedicated for dietary fiber in reduction needle mushroom, while improving needle mushroom
The nutritional agents of the content of middle vitamin C, carrotene, protein, zinc is also rarely reported.
Invention content
It is an object of the invention to overcome the shortage of prior art, a kind of nutritional agents is provided, especially can effectively reduce needle mushroom
The content of middle dietary fiber, while improving the content of vitamin C in needle mushroom, carrotene, protein, zinc, the purpose of the present invention
It is realized by following technical proposals：The ingredient of nutritional agents and weight proportion are in the present invention：
100-150 parts of high side calanthe petal, ranging from 125-150 parts of preferred weight part.High side calanthe (Latin
Name：Calanthe yuana T.Tang et F.T.Wang) it is orchid family Calanthe plant, up to 70 centimetres, raceme is long
29 centimetres, 14 flowers of sparsely grow, petal skew bolt shape is 15 millimeters long, 5.5 millimeters wide, the high side calanthe petal used in the present invention
Pick up from the evergreen broad-leaved hayashishita of 1800 meters of the high side height above sea level in Sichuan.
260-320 parts of pod Opulus leaves stirrup blades, ranging from 285-315 parts of preferred weight part.Jia Opulus leaf raspberry (Latins
Scientific name：Rubus viburnifolius Focke) it is rose family rubus climbing shrub, Dan Ye, width egg shape to subcircular,
Dilute long avette, 6-11 centimetres long, 5-9.5 centimetres wide, the anxious point in top or short point, base portion section shape to nearly heart are used in of the invention
Pod Opulus leaf stirrup blades pick up from drying hillside fields or the shaw in height above sea level 1800-3000 meters of Yunnan.
300-350 parts of parsley Mang ox seedling fruit, ranging from 325-345 parts of preferred weight part.Parsley Mang ox seedling (Latin
Scientific name：Erodium cicutarium (L.) L ' Herit.) it is that MangNiu Er seedlings section Mang ox seedling belongs to annual or biennial herb,
10-20 centimetres high, capsule is 2-4 centimetres long, by short volt hair, the fruiting period 7-10 months, the parsley Mang ox seedling fruit used in of the invention
Pick up from Northwest Sichuan mountainous region gravel matter hillside.
550 parts of water.
Production method of the present invention is as follows：
(1) high side calanthe petal, pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit is taken to impregnate 36 in 40 DEG C of water
It is spare after hour；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
The ultrasonic extraction instrument brand used in the present invention is opened up for sea, model HI-T00, and it is limited to open up mechanical science and technology purchased from sea
Company；Centrifuge brand is Hunan instrument, and model H2100R is purchased from Xiang Yi centrifuges Instrument Ltd.；High-pressure steam sterilizing pan
Brand is four directions, and model MJQ50-1 is purchased from Xuzhou four directions Medical Devices Co., Ltd.；Shaking table brand is permanent promise, model
HWS-250B makes the science and technology prosperous Co., Ltd purchased from Beijing's perseverance promise profit.
Experiment process：The place for cultivating needle mushroom is Nanchang area mushroom cultivation base, and cultivation kind is needle mushroom
No. 12.Prepare 300 1100 milliliters of edible fungi cultivating bottle and be used for soilless culture, is divided into 14 groups of test groups and 1 group of control group, often
20 bottles of group, and number, 800 milliliters of needle mushroom special culture solutions of every bottle of injection, culture solution is conventional cultivation needle mushroom culture solution,
In contain 100 grams of potato, 15 grams of brown sugar, 10 grams of glucose, 40 grams of wheat bran, 2.0 grams of peptone, 2.0 grams of potassium dihydrogen phosphate, sulfuric acid
1.0 grams of magnesium, vitamin B1 is a piece of, and 0.3 milliliter of polyoxypropylene glyceryl is dissolved in water and is settled to 1 liter, this matches for needle mushroom culture solution
Side, formula and its preparation method those skilled in the art should know；Prepare 300 needle mushroom culture special plastic bags (long 17
Centimetre, it is 28 centimetres wide, 0.6 millimeter thick), it is divided into 14 groups of test groups and 1 group of control group, every group 20 bags, and number, often packed culture
300 grams of material, compost are conventional cultivation needle mushroom compost, and with corncob 73%, wheat bran 25%, gypsum 1% is constituted, will be above-mentioned
Compost adds water fully to mix thoroughly, then adjusts pH value to 6 or so, and water content, which with hand pinches material and has water droplet to ooze place but do not form flow, is
Preferably, each polybag feeds 300 grams after compost prepares, both ends tying.The culture material formulas such as wheat bran, corncob, gypsum and its
Preparation method those skilled in the art should know.It was inoculated with October 1, culture bottle and cultivating bag is put into sterile training after inoculation
Foster room is cultivated, and test group culture bottle is in 100 milliliters of nutritional agents of the invention of every bottle of injection on October 15, control group culture bottle
It is not injected into the nutritional agents of the present invention, test group cultivating bag is in 80 milliliters of nutritional agents of the invention of every bag of injection on October 15, control
Group cultivating bag is not injected into the nutritional agents of the present invention, test group and control group golden mushroom plantation administrative skill and is planted with conventional needle mushroom
It manages identical, is the prior art, those skilled in the art should know.After 1-December 1 November harvests, every group random
1kg needle mushrooms are chosen, the inspection of dietary fiber content, Vitamin C content, carotene carotene content, protein content and Zn content is carried out
It surveys, and calculates per dietary fiber content, Vitamin C content, carotene carotene content, the protein content contained in 100g needle mushrooms
And the average value and record of Zn content, dietary fiber content, Vitamin C content, carrot in needle mushroom collecting method, needle mushroom
Cellulose content, the detection method of protein content and Zn content, sample treatment and instrument are the prior art, and dietary fiber contains
Amount is measured as enzyme-gravimetric method, and the assay method of Vitamin C content is permanganimetric method, the measurement side of carotene carotene content
Method is paper chromatography, and the assay method of protein content is Coomassie Brilliant Blue, and the assay method of Zn content is ashing method, ability
Field technique personnel should know.
Specific implementation mode
Experiment process：The place for cultivating needle mushroom is Nanchang area mushroom cultivation base, and cultivation kind is needle mushroom
No. 12.Prepare 300 1100 milliliters of edible fungi cultivating bottle and be used for soilless culture, is divided into 14 groups of test groups and 1 group of control group, often
20 bottles of group, and number, 800 milliliters of needle mushroom special culture solutions of every bottle of injection, culture solution is conventional cultivation needle mushroom culture solution,
In contain 100 grams of potato, 15 grams of brown sugar, 10 grams of glucose, 40 grams of wheat bran, 2.0 grams of peptone, 2.0 grams of potassium dihydrogen phosphate, sulfuric acid
1.0 grams of magnesium, vitamin B1 is a piece of, and 0.3 milliliter of polyoxypropylene glyceryl is dissolved in water and is settled to 1 liter, this matches for needle mushroom culture solution
Side, formula and its preparation method those skilled in the art should know；Prepare 300 needle mushroom culture special plastic bags (long 17
Centimetre, it is 28 centimetres wide, 0.6 millimeter thick), it is divided into 14 groups of test groups and 1 group of control group, every group 20 bags, and number, often packed culture
300 grams of material, compost are conventional cultivation needle mushroom compost, and with corncob 73%, wheat bran 25%, gypsum 1% is constituted, will be above-mentioned
Compost adds water fully to mix thoroughly, then adjusts pH value to 6 or so, and water content, which with hand pinches material and has water droplet to ooze place but do not form flow, is
Preferably, each polybag feeds 300 grams after compost prepares, both ends tying.The culture material formulas such as wheat bran, corncob, gypsum and its
Preparation method those skilled in the art should know.It was inoculated with October 1, culture bottle and cultivating bag is put into sterile training after inoculation
Foster room is cultivated, and test group culture bottle is in 100 milliliters of nutritional agents of the invention of every bottle of injection on October 15, control group culture bottle
It is not injected into the nutritional agents of the present invention, test group cultivating bag is in 80 milliliters of nutritional agents of the invention of every bag of injection on October 15, control
Group cultivating bag is not injected into the nutritional agents of the present invention, test group and control group golden mushroom plantation administrative skill and is planted with conventional needle mushroom
It manages identical, is the prior art, those skilled in the art should know.After 1-December 1 November harvests, every group random
1kg needle mushrooms are chosen, the inspection of dietary fiber content, Vitamin C content, carotene carotene content, protein content and Zn content is carried out
It surveys, and calculates per dietary fiber content, Vitamin C content, carotene carotene content, the protein content contained in 100g needle mushrooms
And the average value and record of Zn content, dietary fiber content, Vitamin C content, carrot in needle mushroom collecting method, needle mushroom
Cellulose content, the detection method of protein content and Zn content, sample treatment and instrument are the prior art, and dietary fiber contains
Amount is measured as enzyme-gravimetric method, and the assay method of Vitamin C content is permanganimetric method, the measurement side of carotene carotene content
Method is paper chromatography, and the assay method of protein content is Coomassie Brilliant Blue, and the assay method of Zn content is ashing method, ability
Field technique personnel should know.
Embodiment 1
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 145 parts by weight of side calanthe petal, 310 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 345 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.85 grams of dietary fiber, vitamin C 2.61 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 39.3 microgram of carrotene, 3.14 grams of protein, 0.51 milligram of zinc.
Contain 1.86 grams of dietary fiber, 2.60 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
39.1 micrograms of Bu Su, 3.12 grams of protein, 0.52 milligram of zinc.
Embodiment 2
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 150 parts by weight of side calanthe petal, 285 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 335 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.82 grams of dietary fiber, vitamin C 2.62 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 39.0 microgram of carrotene, 3.13 grams of protein, 0.50 milligram of zinc.
Contain 1.83 grams of dietary fiber, 2.60 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
38.8 micrograms of Bu Su, 3.12 grams of protein, 0.49 milligram of zinc.
Embodiment 3
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 125 parts by weight of side calanthe petal, 315 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 325 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.83 grams of dietary fiber, vitamin C 2.59 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 39.2 microgram of carrotene, 3.11 grams of protein, 0.53 milligram of zinc.
Contain 1.80 grams of dietary fiber, 2.58 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
38.9 micrograms of Bu Su, 3.13 grams of protein, 0.52 milligram of zinc.
Embodiment 4
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 135 parts by weight of side calanthe petal, 305 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 340 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.86 grams of dietary fiber, vitamin C 2.58 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 39.1 microgram of carrotene, 3.12 grams of protein, 0.47 milligram of zinc.
Contain 1.85 grams of dietary fiber, 2.59 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
38.6 micrograms of Bu Su, 3.10 grams of protein, 0.50 milligram of zinc.
Embodiment 5
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 100 parts by weight of side calanthe petal, 280 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 315 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.84 grams of dietary fiber, vitamin C 2.60 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 39.2 microgram of carrotene, 3.13 grams of protein, 0.50 milligram of zinc.
Contain 1.85 grams of dietary fiber, 2.62 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
38.7 micrograms of Bu Su, 3.14 grams of protein, 0.47 milligram of zinc.
Embodiment 6
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 120 parts by weight of side calanthe petal, 260 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 350 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.87 grams of dietary fiber, 2.55 milli of vitamin C in 20 culture bottles in average every 100 grams of pulp
Gram, 39.4 microgram of carrotene, 3.10 grams of protein, 0.46 milligram of zinc.
Contain 1.85 grams of dietary fiber, 2.63 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
39.2 micrograms of Bu Su, 3.15 grams of protein, 0.47 milligram of zinc.
Embodiment 7
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 130 parts by weight of side calanthe petal, 270 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 320 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.85 grams of dietary fiber, vitamin C 2.59 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 38.8 microgram of carrotene, 3.15 grams of protein, 0.47 milligram of zinc.
Contain 1.87 grams of dietary fiber, 2.55 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
38.4 micrograms of Bu Su, 3.16 grams of protein, 0.50 milligram of zinc.
Embodiment 8
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 105 parts by weight of side calanthe petal, 320 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after real 310 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) high side calanthe petal, pod Opulus leaf stirrups blades, 4 DEG C of the parsley Mang ox seedling fruit obtained (1) step
Ultrasonic extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, temperature setting 4
DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 1.84 grams of dietary fiber, vitamin C 2.58 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 38.5 microgram of carrotene, 3.11 grams of protein, 0.46 milligram of zinc.
Contain 1.81 grams of dietary fiber, 2.57 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
38.2 micrograms of Bu Su, 3.14 grams of protein, 0.48 milligram of zinc.
Embodiment 9
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) spare after taking high 145 parts by weight of side calanthe petal to be impregnated 36 hours in 40 DEG C of water；
(2) high 4 DEG C of the side calanthe petal ultrasonic extraction 5 hours for obtaining (1) step, centrifuge is put into after taking-up
30 minutes, rotating speed was set as 15000 turns per minute, and temperature setting is 4 DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 2.55 grams of dietary fiber, vitamin C 2.11 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 31.6 microgram of carrotene, 2.72 grams of protein, 0.40 milligram of zinc.
Contain 2.56 grams of dietary fiber, 2.10 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
31.4 micrograms of Bu Su, 2.70 grams of protein, 0.39 milligram of zinc.
Embodiment 10
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) spare after taking 310 parts by weight of pod Opulus leaf stirrups blades to be impregnated 36 hours in 40 DEG C of water；
(2) pod Opulus leaf stirrups 4 DEG C of the blades ultrasonic extraction 5 hours for obtaining (1) step, be put into after taking-up centrifuge from
The heart 30 minutes, rotating speed are set as 15000 turns per minute, and temperature setting is 4 DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 2.60 grams of dietary fiber, vitamin C 2.13 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 32.0 microgram of carrotene, 2.69 grams of protein, 0.41 milligram of zinc.
Contain 2.61 grams of dietary fiber, 2.14 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
31.9 micrograms of Bu Su, 2.65 grams of protein, 0.40 milligram of zinc.
Embodiment 11
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) spare after taking 345 parts by weight of parsley Mang ox seedling fruit to be impregnated 36 hours in 40 DEG C of water；
(2) parsley Mang ox seedling 4 DEG C of the fruit ultrasonic extraction 5 hours for obtaining (1) step, be put into after taking-up centrifuge from
The heart 30 minutes, rotating speed are set as 15000 turns per minute, and temperature setting is 4 DEG C, after precipitation is discarded, stay supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 2.54 grams of dietary fiber, vitamin C 2.14 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 30.9 microgram of carrotene, 2.74 grams of protein, 0.42 milligram of zinc.
Contain 2.56 grams of dietary fiber, 2.13 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
31.0 micrograms of Bu Su, 2.71 grams of protein, 0.39 milligram of zinc.
Embodiment 12
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 145 parts by weight of side calanthe petal, 310 parts by weight of pod Opulus leaf stirrups blades is taken to be impregnated in 40 DEG C of water
It is spare after 36 hours；
(2) the high side calanthe petal that obtains (1) step, pod Opulus leaf stirrups 4 DEG C of blades ultrasonic extraction 5 hours, take
Centrifuge is put into after going out 30 minutes, rotating speed is set as 15000 turns per minute, and temperature setting is 4 DEG C, after precipitation is abandoned
It goes, stays supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 2.28 grams of dietary fiber, vitamin C 2.35 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 33.7 microgram of carrotene, 2.94 grams of protein, 0.43 milligram of zinc.
Contain 2.29 grams of dietary fiber, 2.34 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
33.9 micrograms of Bu Su, 2.90 grams of protein, 0.42 milligram of zinc.
Embodiment 13
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) 310 parts by weight of pod Opulus leaf stirrups blades, 345 parts by weight of parsley Mang ox seedling fruit is taken to be soaked in 40 DEG C of water
Bubble is spare after 36 hours；
(2) the pod Opulus leaf stirrups blades that obtain (1) step, parsley Mang ox seedling 4 DEG C of fruit ultrasonic extraction 5 hours,
Centrifuge is put into after taking-up 30 minutes, rotating speed is set as 15000 turns per minute, and temperature setting is 4 DEG C, after will precipitation
It discards, stays supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 2.30 grams of dietary fiber, vitamin C 2.36 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 33.9 microgram of carrotene, 2.96 grams of protein, 0.44 milligram of zinc.
Contain 2.32 grams of dietary fiber, 2.33 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
33.5 micrograms of Bu Su, 2.90 grams of protein, 0.43 milligram of zinc.
Embodiment 14
A kind of preparation method of needle mushroom nutrient special, includes the following steps：
(1) high 145 parts by weight of side calanthe petal, 345 parts by weight of parsley Mang ox seedling fruit is taken to be impregnated in 40 DEG C of water
It is spare after 36 hours；
(2) the high side calanthe petal that obtains (1) step, parsley Mang ox seedling 4 DEG C of fruit ultrasonic extraction 5 hours, take
Centrifuge is put into after going out 30 minutes, rotating speed is set as 15000 turns per minute, and temperature setting is 4 DEG C, after precipitation is abandoned
It goes, stays supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing is gone out through high steam
It after 121 DEG C of 1.0kpa of bacterium pot sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, culture 20 days, i.e.,
Obtain the nutritional agents.
Test result：Contain 2.26 grams of dietary fiber, vitamin C 2.37 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 33.6 microgram of carrotene, 2.96 grams of protein, 0.44 milligram of zinc.
Contain 2.27 grams of dietary fiber, 2.38 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
33.7 micrograms of Bu Su, 2.94 grams of protein, 0.42 milligram of zinc.
Embodiment 15
The present embodiment is check experiment, and needle mushroom does not apply nutritional agents of the present invention.
Test result：Contain 2.71 grams of dietary fiber, vitamin C 2.02 in average every 100 grams of needle mushrooms in 20 culture bottles
Milligram, 30.0 microgram of carrotene, 2.39 grams of protein, 0.38 milligram of zinc.
Contain 2.72 grams of dietary fiber, 2.04 milligrams of vitamin C, Hu Luo in average every 100 grams of needle mushrooms in 20 cultivating bags
30.2 micrograms of Bu Su, 2.40 grams of protein, 0.37 milligram of zinc.
Claims (2)
1. a kind of needle mushroom nutrient special, it is characterized in that being prepared by the following steps：
(1) high 145 parts by weight of side calanthe petal, 310 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after 345 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) the high side calanthe petal that obtains (1) step, pod Opulus leaf stirrups blades, parsley Mang ox seedling 4 DEG C of ultrasounds of fruit
Extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, and temperature setting is 4 DEG C, knot
Precipitation is discarded after beam, stays supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing, through high-pressure steam sterilizing pan
It after 121 DEG C of 1.0kpa sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, cultivates 20 days to get this
Nutritional agents.
2. a kind of preparation method of needle mushroom nutrient special, includes the following steps and is made：
(1) high 145 parts by weight of side calanthe petal, 310 parts by weight of pod Opulus leaf stirrups blades, parsley Mang ox seedling fruit are taken
It is spare after 345 parts by weight are impregnated 36 hours in 40 DEG C of water；
(2) the high side calanthe petal that obtains (1) step, pod Opulus leaf stirrups blades, parsley Mang ox seedling 4 DEG C of ultrasounds of fruit
Extraction 5 hours, is put into centrifuge 30 minutes after taking-up, rotating speed is set as 15000 turns per minute, and temperature setting is 4 DEG C, knot
Precipitation is discarded after beam, stays supernatant spare；
(3) supernatant that (2) step obtains is placed in water-bath 2 hours in 80 DEG C of water-baths, it is spare；
(4) liquid that (3) step obtains is uniformly mixed with the water of 550 parts by weight, sealed membrane sealing, through high-pressure steam sterilizing pan
It after 121 DEG C of 1.0kpa sterilize 30 minutes, is placed on shaking table, rotating speed 120r/min, temperature is 40 DEG C, cultivates 20 days to get this
Nutritional agents.
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