CN108392459A - A kind of sea cucumber peptide anti-oxidant face mask - Google Patents
A kind of sea cucumber peptide anti-oxidant face mask Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/81—Preparation or application process involves irradiation
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Abstract
The present invention relates to cosmetic fields, and in particular to a kind of sea cucumber peptide anti-oxidant face mask.Including following mass parts substance：15 parts of sea cucumber peptide, 6 26 parts of plant extracts, 57 parts of butanediol, 10 14 parts of polyethylene glycol, 13 parts of hyaluronic acid, 12 parts of carbomer, 0.2 0.4 parts of allantoin, 46 parts of natural antiseptic agent.The present invention first carries out self-dissolving, then using the enzymolysis liquid after self-dissolving as substrate using sea cucumber albumen as raw material, and protease is added and carries out secondary step-by-step hydrolysis, improves the yield of sea cucumber polypeptide.Other ingredients are added in the sea cucumber polypeptide for preparing of the present invention to be prepared into facial mask and have the effect of anti-oxidant, bright color, desalinate microgroove, the light spot of moisturizing.
Description
Technical field
The present invention relates to cosmetic fields, and in particular to a kind of sea cucumber peptide anti-oxidant face mask.
Background technology
With the improvement of living standards, the problem of women starts increasingly to pay close attention to skin aging, is exposed to sunlight for a long time
Under, the radiation of foul atmosphere, computer and often makeup can all cause skin aging, lead to that complexion is dull, the colour of skin is uneven, skin is dry
The problems such as dry, microgroove and spot, generates.
Sea cucumber is important oceanic invertebrate, existence, based on Stichopus chloronotus (Brandt) and Stichopus variegatus (Sempen) etc..Since ancient times, extra large
Ginseng is exactly nutraceutical and health food.Ming Dynasty's income book on Chinese herbal medicine, Qing Dynasty's Chinese medicine ancient books and records are then classified as help drug, claim " sea cucumber
Warm-natured benefit, sufficient enemy's ginseng " has the benefits of " tonifying primordial Qi, the beneficial vital organs of the human body give birth to through establishing-Yang, promote longevity ".And modern scientific research is demonstrate,proved
It is real, it is beneficial to the nutritional ingredient of human physiological activity in sea cucumber body containing more than 5 kinds, the content of wherein protein is high.Dried sea-cucumber
Gross protein value is about 40.7~63.3% in body wall organic principle, and lipid accounts for about 4%, and polyoses content 6% or so is another to go back
Contain a small amount of nucleic acid.Calcic, magnesium salts and iron, manganese, zinc, copper, molybdenum, selenium and other trace elements in inorganic constituents.Sea cucumber peptide refer to from
The active peptide with special physiological function extracted in sea cucumber, can be by small peptide or molecule that 2~12 amino acid form
Measure the polypeptide of bigger.Its bioavailability is high, is more easy to absorb than amino acid.Although the country has many sea cucumber peptide products, big
It all concentrates in the technique of hydrolysis sea cucumber albumen and the physiological activity of sea cucumber peptide is studied, the application of sea cucumber peptide is also concentrated
Field is eaten in medicine, it is less in the research of cosmetics related field.
Technical solution
In view of the above problems, invention using sea cucumber albumen as raw material, first carries out self-dissolving, then with the enzymolysis after self-dissolving
Liquid is substrate, and protease is added and carries out secondary step-by-step hydrolysis, improves the yield of sea cucumber polypeptide；Sea cucumber prepared by the present invention is more
Peptide be added other ingredients be prepared into facial mask have the effect of anti-oxidant, bright color, desalination microgroove, the light spot of moisturizing.
For achieving the above object, the present invention takes following technical scheme：
A kind of sea cucumber peptide anti-oxidant face mask, including following mass parts substance：1-5 parts of sea cucumber peptide, 6-26 parts of plant extracts,
5-7 parts of butanediol, 10-14 parts of polyethylene glycol, 1-3 parts of hyaluronic acid, 1-2 parts of carbomer, 0.2-0.4 parts of allantoin are natural anti-
Rotten agent 4-6 parts.
Preferably, including following mass parts substance：2-4 parts of sea cucumber peptide, 11-21 parts of plant extracts, butanediol 5.5-6.5
Part, 11-13 parts of polyethylene glycol, 1.5-2.5 parts of hyaluronic acid, 1.2-1.8 parts of carbomer, 0.25-0.35 parts of allantoin are natural anti-
Rotten agent 4.5-5.5 parts.
Preferably, including following mass parts substance：3 parts of sea cucumber peptide, 16 parts of plant extracts, 6 parts of butanediol, polyethylene glycol
12 parts, 2 parts of hyaluronic acid, 1.5 parts of carbomer, 0.3 part of allantoin, 5 parts of natural antiseptic agent.
The plant extracts is：Semen Coicis extract, pot marigold extract, aloe extract, grape seed extract, grape
Bark extract, it is at least three kinds of in lemon extract.
The natural antiseptic agent is forsythia suspense extraction, Rosmarinus officinalis extract, thyme extract, any of extractive of perilla
Kind.
A method of the sea cucumber peptide used in sea cucumber peptide anti-oxidant face mask is prepared, is comprised the steps of：
A) new fresh sea cucumber is cleaned to be kept separately body wall and alimentary canal after solution is dug and is smashed to pieces through bruisher, after colloidal grinding
Body wall homogenate mixed with the digestive juice that ice bath is ground, add water adjust solid-liquid ratio, irradiate certain time under 30W ultraviolet lamps
Afterwards, suitable temperature, pH are adjusted, self-dissolving is carried out in hydrolysis reactor；
B) boiling water bath enzyme deactivation is carried out after self-dissolving, then rotary evaporation is concentrated to suitable solid-liquid ratio, adjusts pH, temperature
Degree, is added a certain amount of compound protease and carries out secondary hydrolysis in hydrolysis reactor；
C) boiling water bath enzyme deactivation is finally used, it is cooling to be placed on 5000r/min centrifugations 10min in high speed freezing centrifuge, it takes
Clear liquid, freeze-drying is to get sea cucumber polypeptide after concentration.
Process conditions is selected as in step a：From 40-60 DEG C of solubility temperature, hydrolysis time 2-6h, ultraviolet irradiation time
15-35min, pH7-9, solid-liquid ratio 1:3-1:5.
Preferably, process conditions are preferably in step a：Self-dissolving temperature 50 C, hydrolysis time 4h, ultraviolet irradiation time
25min, pH8, solid-liquid ratio 1:4.
Technique is selected as in step b：50 DEG C of hydrolysis temperature, enzymolysis time 3h, pH10, solid-liquid ratio 1:5, ultraviolet light irradiation
Time 25min.
Beneficial effects of the present invention are：
(1) present invention first carries out self-dissolving, then using the enzymolysis liquid after self-dissolving as substrate using sea cucumber albumen as raw material, and egg is added
White enzyme carries out secondary step-by-step hydrolysis, improves the yield of sea cucumber polypeptide.
(2) the method for the present invention, the sea cucumber polypeptide dissolubility height of acquisition, viscosity reduce, and thermal stability is good, is produced for sea cucumber polypeptide
The exploitation of product provides the foundation.
(3) other ingredients are added in the sea cucumber polypeptide for preparing of the present invention and are prepared into facial mask with anti-oxidant, bright color, light
Change the effect of microgroove, the light spot of moisturizing.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of sea cucumber peptide anti-oxidant face mask, including following mass parts substance：1 part of sea cucumber peptide, 6 parts of plant extracts, fourth two
5 parts of alcohol, 10 parts of polyethylene glycol, 1 part of hyaluronic acid, 1 part of carbomer, 0.2 part of allantoin, 4 parts of natural antiseptic agent.
The plant extracts is：Semen Coicis extract, pot marigold extract, aloe extract, grape seed extract, grape
Bark extract, it is at least three kinds of in lemon extract.
The natural antiseptic agent is forsythia suspense extraction, Rosmarinus officinalis extract, thyme extract, any of extractive of perilla
Kind.
A method of the sea cucumber peptide used in sea cucumber peptide anti-oxidant face mask is prepared, is comprised the steps of：
A) new fresh sea cucumber is cleaned to be kept separately body wall and alimentary canal after solution is dug and is smashed to pieces through bruisher, after colloidal grinding
Body wall homogenate mixed with the digestive juice that ice bath is ground, add water adjust solid-liquid ratio, irradiate certain time under 30W ultraviolet lamps
Afterwards, suitable temperature, pH are adjusted, self-dissolving is carried out in hydrolysis reactor；
B) boiling water bath enzyme deactivation is carried out after self-dissolving, then rotary evaporation is concentrated to suitable solid-liquid ratio, adjusts pH, temperature
Degree, is added a certain amount of compound protease and carries out secondary hydrolysis in hydrolysis reactor；
C) boiling water bath enzyme deactivation is finally used, it is cooling to be placed on 5000r/min centrifugations 10min in high speed freezing centrifuge, it takes
Clear liquid, freeze-drying is to get sea cucumber polypeptide after concentration.
Process conditions is selected as in step a：From 40-60 DEG C of solubility temperature, hydrolysis time 2-6h, ultraviolet irradiation time
15-35min, pH7-9, solid-liquid ratio 1:3-1:5.
Preferably, process conditions are preferably in step a：Self-dissolving temperature 50 C, hydrolysis time 4h, ultraviolet irradiation time
25min, pH8, solid-liquid ratio 1:4.
Technique is selected as in step b：50 DEG C of hydrolysis temperature, enzymolysis time 3h, pH10, solid-liquid ratio 1:5, ultraviolet light irradiation
Time 25min.
Embodiment 2
A kind of sea cucumber peptide anti-oxidant face mask, including following mass parts substance：2 parts of sea cucumber peptide, 11 parts of plant extracts, fourth two
5.5 parts of alcohol, 11 parts of polyethylene glycol, 1.5 parts of hyaluronic acid, 1.2 parts of carbomer, 0.25 part of allantoin, 4.5 parts of natural antiseptic agent.
Embodiment 3
A kind of sea cucumber peptide anti-oxidant face mask, including following mass parts substance：3 parts of sea cucumber peptide, 16 parts of plant extracts, fourth two
6 parts of alcohol, 12 parts of polyethylene glycol, 2 parts of hyaluronic acid, 1.5 parts of carbomer, 0.3 part of allantoin, 5 parts of natural antiseptic agent.
Embodiment 4
A kind of sea cucumber peptide anti-oxidant face mask, including following mass parts substance：4 parts of sea cucumber peptide, 21 parts of plant extracts, fourth two
6.5 parts of alcohol, 13 parts of polyethylene glycol, 2.5 parts of hyaluronic acid, 1.8 parts of carbomer, 0.35 part of allantoin, 5.5 parts of natural antiseptic agent.
Embodiment 5
A kind of sea cucumber peptide anti-oxidant face mask, including following mass parts substance：5 parts of sea cucumber peptide, 26 parts of plant extracts, fourth two
7 parts of alcohol, 14 parts of polyethylene glycol, 3 parts of hyaluronic acid, 2 parts of carbomer, 0.4 part of allantoin, 6 parts of natural antiseptic agent.
Experimental result detects
1. the measurement of sea cucumber polypeptide scavenging ability of DPPH free radical
DPPH free radicals are a kind of very stable free radicals because the free radical in organism hold time it is too short,
The free radical substituted in common organism is generated free radicals using DPPH, the energy of free radical is captured for assessing polyphenoils
Power.
With reference to Sharma etc. assay method and slightly adjust.The sea cucumber polypeptide solution of 1mL various concentrations is drawn in test tube
In, the DPPH for the 0.1mmol/L that 4mL is prepared with 95% ethyl alcohol is added, mixing stands 30min at room temperature, in wavelength 517nm
Lower measurement OD, is denoted as A.Sample is calculated to the clearance rate of DPPH free radicals by formula (1), the results are shown in Table 1.
Y=(1- (A-A1)/A0) × 100% (1)
In formula：A is the OD values that sample solution is added；
A1The absorbance value of DPPH is replaced by 95% isometric ethyl alcohol；
A0The absorbance value of sample solution is replaced by isometric distilled water.
1 sea cucumber polypeptide of table removes DEEP free radical abilities
| Concentration/mg/mL | 2 | 4 | 6 | 8 | 10 |
| Clearance rate/% | 22 | 41 | 52 | 58 | 72 |
As shown in Table 1, with the raising of sea cucumber polypeptide concentration, scavenging ability of DPPH free radical also gradually increases, in concentration
For 10mg/mL when, clearance rate reaches 72%, illustrate the sea cucumber polypeptide extracted by the method for the invention to DPPH free radicals have it is good
Good Scavenging activity.
2. the measurement of sea cucumber polypeptide scavenging hydroxyl ability
Hydroxy radical is, harm maximum a kind of freedom most strong to organismal toxicity in the active oxygen radical being currently known
Base, it can be acted on the different kinds of molecules in organism in several ways, cause amino acid, protein, nucleic acid and lipid etc.
The oxidative damage of substance, so as to cause the disorder of body function.
Configuration concentration is the FeSO of 9mmol/L4, salicylic acid-ethanol solution of 9mmol/L and the H of 8.8mmol/L2O2.It is accurate
The standby sea cucumber polypeptide solution for measuring 1mL different quality concentration, is separately added into FeSO4With each 1mL of salicylic acid-ethanol solution, finally
The H of 1mL is added2O2, mixing reacts 1h at 37 DEG C, and absorbance is measured at 510nm.By formula (2) calculate sample to hydroxyl from
By the clearance rate of base, 2 are the results are shown in Table.
Y=(1- (A1-A2)/A0) × 100% (2)
In formula：A1For the OD values of sample solution are added；
A2H is replaced by isometric distilled water2O2Absorbance value；
A0The absorbance value of sample solution is replaced by isometric distilled water.
2 sea cucumber polypeptide scavenging hydroxyl ability of table
| Concentration/mg/mL | 3 | 5 | 10 | 15 | 20 | 25 |
| Clearance rate/% | 45 | 57 | 63 | 72 | 77 | 80 |
As shown in Table 2 with the raising of sea cucumber polypeptide concentration, scavenging hydroxyl ability also gradually increases, in concentration 3-
In the range of 15mg/mL, Scavenging activity increases more rapid, and increase becomes slow later.
3. sea cucumber polypeptide removes the measurement of ABTS free radical abilities
ABTS free radicals show good dissolubility in water and alcoholic solvent, therefore are usually used in evaluation water solubility and fat
The antioxidant activity of soluble natural product mainly measures the ability that antioxidant keeps redox state.
With reference to the assay method of Su etc..By the potassium sulfite mixed in equal amounts of the ABTS of 7mmol/L and 4.8mmol/L, in room
Warm avoid light place 12-16h, you can obtain ABTS free radical storing solutions.ABTS free radical storing solutions, which are diluted to absorbance value, is
(0.700 ± 0.001) it is used as working solution.The sea cucumber polypeptide solution 0.2mL for drawing different quality concentration respectively has plug examination in 10mL
Guan Zhong is added the ABTS working solutions of 3.8mL, shakes up, and stands the absorbance value of measurement solution after 6min at room temperature, and wavelength is
734nm.Sample is calculated to the clearance rate of ABTS free radicals by formula (3), the results are shown in Table 3.
Y=(1- (A1-A2)/A0) × 100% (3)
In formula：A1For the OD values of sample solution are added；
A2The absorbance value of ABTS free radical working solutions is replaced by isometric distilled water；
A0The absorbance value of sample solution is replaced by isometric distilled water.
3 sea cucumber polypeptide of table removes ABTS free radical abilities
| Concentration/mg/mL | 3 | 5 | 10 | 15 | 20 | 25 |
| Clearance rate/% | 22 | 37 | 58 | 87 | 92 | 93 |
As shown in Table 3, with the raising of sea cucumber polypeptide concentration, ABTS free radical abilities is removed and are also gradually increased, in concentration
In the range of 3-15mg/mL, Scavenging activity increases more rapid, and increase becomes slow later.
4. sea cucumber polypeptide removes the measurement of superoxide anion ability
Superoxide anion is a kind of typical free radical in active oxygen, and human body is interior there are certain quantity, does not change
Learn harmless when variation, but have stronger oxidation toxicity with the products upon cell after hydroxy combining, aging with human body and
The generation of the diseases such as cancer is related.
The phosphate buffer (pH 8.04) of a concentration of 0.1mol/L of 3.5mL is measured in test tube, 1mL distilled water is added,
10mmol/L pyrogallol solution 0.5mL, immediately timing and rapid mixing measure absorbance after 0.5min at wavelength 325nm
It is worth (A1), a data are recorded to 3.5min (A every 30s2), replace pyrogallol as blank control using 10mmol/L hydrochloric acid.
Calculate mouse thymus cells rate.The accurate sea cucumber polypeptide solution for measuring 1mL different quality concentration is surveyed according to the method described above
The absorbance value of solution after fixed sample-adding is calculated mouse thymus cells rate after being loaded by formula (4) and (5), the results are shown in Table 4.
S0=(A2-A1)/3 (4)
Y=((S0-S1)/S0) * 100% (5)
In formula：S0For mouse thymus cells rate；
A2For the absorbance value of 3.5min；
A1For the absorbance value of 0.5min；
S1For mouse thymus cells rate after sample is added.
4 sea cucumber polypeptide of table removes superoxide anion ability
| Concentration/mg/mL | 3 | 5 | 10 | 15 | 20 |
| Clearance rate/% | 18 | 20 | 38 | 41 | 58 |
As shown in Table 4, with the raising of sea cucumber polypeptide concentration, clear superoxide anion ability also gradually increases, a concentration of
When 20mg/mL, clearance rate reaches 58%, and it is good to illustrate that the sea cucumber polypeptide extracted by the method for the invention has superoxide anion
Scavenging activity.
5. normal individual trial is tested
Preferably to prove that it is excellent that embodiment facial mask provided by the present invention has the effect of, chooses embodiment 1-5 and carry out
Further investigation on probation and evaluation test.Embodiment is as follows：250 triers are chosen, age distribution is 28-46 Sui, with
Machine is divided into 5 groups, carries out evaluation test to embodiment, does not during which use other skin care item, and testing items after one month on probation refers to
Mark, the results are shown in Table 5.
5 embodiment 1-5 indices results of table
As shown in Table 5, it has used after the facial mask of sea cucumber peptide is added for a period of time, most of trier dry skin and aging
The phenomenon that have and be obviously improved, part trier's skin aging state is alleviated, and it is good to illustrate that facial mask of the present invention has
Anti-oxidant, anti-aging effects.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Any one skilled in the art is in the technical scope of present disclosure, the change or replacement that can be readily occurred in,
It should all be included within the scope of the present invention.Therefore, protection scope of the present invention should be with the protection of claims
Subject to range.
Claims (9)
1. a kind of sea cucumber peptide anti-oxidant face mask, which is characterized in that include following mass parts substance：1-5 parts of sea cucumber peptide, plant extract
6-26 parts of object, 5-7 parts of butanediol, 10-14 parts of polyethylene glycol, 1-3 parts of hyaluronic acid, 1-2 parts of carbomer, allantoin 0.2-0.4
Part, 4-6 parts of natural antiseptic agent.
2. a kind of sea cucumber peptide anti-oxidant face mask according to claim 1, which is characterized in that include following mass parts substance：
2-4 parts of sea cucumber peptide, 11-21 parts of plant extracts, 5.5-6.5 parts of butanediol, 11-13 parts of polyethylene glycol, hyaluronic acid 1.5-2.5
Part, 1.2-1.8 parts of carbomer, 0.25-0.35 parts of allantoin, 4.5-5.5 parts of natural antiseptic agent.
3. a kind of sea cucumber peptide anti-oxidant face mask according to claim 2, which is characterized in that include following mass parts substance：
3 parts of sea cucumber peptide, 16 parts of plant extracts, 6 parts of butanediol, 12 parts of polyethylene glycol, 2 parts of hyaluronic acid, 1.5 parts of carbomer, allantois
0.3 part of element, 5 parts of natural antiseptic agent.
4. according to a kind of any one of claim 1-3 sea cucumber peptide anti-oxidant face masks, which is characterized in that the plant extracts
For：Semen Coicis extract, pot marigold extract, aloe extract, grape seed extract, grape skin extract, in lemon extract
It is at least three kinds of.
5. according to a kind of sea cucumber peptide anti-oxidant face mask of claim 1-3 any one of them, which is characterized in that the natural anticorrosion
Agent be forsythia suspense extraction, Rosmarinus officinalis extract, thyme extract, extractive of perilla it is any.
6. a kind of method preparing the sea cucumber peptide as described in claim any one of 1-3 used in sea cucumber peptide anti-oxidant face mask,
It is characterized in that comprising the steps of：
A) will new fresh sea cucumber clean solution plane after body wall and alimentary canal are kept separately smashed to pieces through bruisher, the body after colloidal grinding
Wall homogenate is mixed with the digestive juice that ice bath is ground, and water is added to adjust solid-liquid ratio, is irradiated after a certain period of time, is adjusted under 30W ultraviolet lamps
Suitable temperature, pH are saved, self-dissolving is carried out in hydrolysis reactor；
B) boiling water bath enzyme deactivation is carried out after self-dissolving, then rotary evaporation is concentrated to suitable solid-liquid ratio, adjusts pH, temperature, adds
Enter a certain amount of compound protease and carries out secondary hydrolysis in hydrolysis reactor；
C) boiling water bath enzyme deactivation is finally used, it is cooling to be placed on 5000r/min centrifugations 10min in high speed freezing centrifuge, supernatant is taken,
Freeze-drying is to get sea cucumber polypeptide after concentration.
7. the preparation method of sea cucumber peptide according to claim 6, which is characterized in that process conditions is selected as in step a：From
40-60 DEG C of solubility temperature, hydrolysis time 2-6h, ultraviolet irradiation time 15-35min, pH7-9, solid-liquid ratio 1:3-1:5.
8. the preparation method of sea cucumber peptide according to claim 7, which is characterized in that process conditions are preferably in step a：Self-dissolving
Temperature 50 C, hydrolysis time 4h, ultraviolet irradiation time 25min, pH8, solid-liquid ratio 1:4.
9. the preparation method of sea cucumber peptide according to claim 6, which is characterized in that technique is selected as in step b：Enzymolysis temperature
50 DEG C, enzymolysis time 3h, pH10 of degree, solid-liquid ratio 1:5, ultraviolet irradiation time 25min.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109106665A (en) * | 2018-08-31 | 2019-01-01 | 青岛银色世纪健康产业集团有限公司 | A kind of Marine biologic peptide anti-acne repairs facial mask and preparation method thereof |
| CN110354019A (en) * | 2019-09-02 | 2019-10-22 | 烟台东宇海珍品有限公司 | A kind of sea cucumber polypeptide freckle removing and whitening moisture saver mask and preparation method thereof |
| CN113813195A (en) * | 2021-11-11 | 2021-12-21 | 佛山科学技术学院 | Shampoo containing sea cucumber peptide and preparation method thereof |
| CN114184584A (en) * | 2021-12-06 | 2022-03-15 | 滨州学院 | Enzyme-determination-based micelle liquid and method for evaluating antioxidant activity of marine natural product |
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| CN104161699A (en) * | 2014-07-04 | 2014-11-26 | 大连美乐生物技术开发有限公司 | Sea cucumber polypeptide young keeping mask and preparation method thereof |
| CN104450845A (en) * | 2014-11-25 | 2015-03-25 | 渤海大学 | Method for preparing sea cucumber polypeptide from sea cucumber endogenous protease |
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| CN104161699A (en) * | 2014-07-04 | 2014-11-26 | 大连美乐生物技术开发有限公司 | Sea cucumber polypeptide young keeping mask and preparation method thereof |
| CN104450845A (en) * | 2014-11-25 | 2015-03-25 | 渤海大学 | Method for preparing sea cucumber polypeptide from sea cucumber endogenous protease |
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| CN113813195A (en) * | 2021-11-11 | 2021-12-21 | 佛山科学技术学院 | Shampoo containing sea cucumber peptide and preparation method thereof |
| CN114184584A (en) * | 2021-12-06 | 2022-03-15 | 滨州学院 | Enzyme-determination-based micelle liquid and method for evaluating antioxidant activity of marine natural product |
| CN114184584B (en) * | 2021-12-06 | 2024-06-04 | 山东航空学院 | Enzyme determination micelle liquid and marine natural product antioxidant activity evaluation method |
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