CN108371008A - A kind of implantation methods of the good bletilla striata of quality - Google Patents
A kind of implantation methods of the good bletilla striata of quality Download PDFInfo
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- CN108371008A CN108371008A CN201610959505.XA CN201610959505A CN108371008A CN 108371008 A CN108371008 A CN 108371008A CN 201610959505 A CN201610959505 A CN 201610959505A CN 108371008 A CN108371008 A CN 108371008A
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- bletilla striata
- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses preparation, seed treatment, sowing, hardening, selection of land site preparation, field planting, greenhouse construction and the field management of a kind of implantation methods of the good bletilla striata of quality, including environment disinfected, culture medium.When the method for the present invention cultivates bletilla striata seeds, bletilla striata seedling percent is high, and the seed sprouting period is short, and obtained bletilla polysaccharide content is high, and bletilla striata quality is good.
Description
Technical field
The present invention relates to a kind of implantation methods of the bletilla striata, especially a kind of implantation methods for the bletilla striata that polyoses content is high.
Background technology
The bletilla striata is the dry rhizome of the orchid family bletilla striata platymiscium bletilla striata.Alias：Even and grass, Gan Gen, give free of charge, Zhu Lan, purple orchid.
Conventional Chinese medicine with functions such as tonifying lung hemostasis, detumescence and promoting granulations.Pulmonary tuberculosis hemoptysis, bronchiectasis hemoptysis, gastric ulcer is cured mainly to spit
Blood, hematuria, the diseases such as have blood in stool；The diseases such as traumatic hemorrhage, burn and scald, rhagadia manus et pedis are controlled in external application.Bletilla striata haemostatic effect spy is good, now with white
The glued membrane block that splendid achnatherum is done is attached to knife-edge part for liver and spleen operation, and instead of blood pliers, effect spy is good.It, can generation with rapid blood coagulation effect
Blood plasma is chiefly used in surgical operation.Main product has cultivation in the provinces such as Sichuan, Yunnan, Shaanxi, Gansu, all parts of the country.
Currently, wild bletilla striata is increasingly exhausted, meet market mainly by artificial growth；Although the bletilla striata has a large amount of seeds, seed
The nonnutritive reserve of short life, sprouting condition are harsh, and Seedling Stage is longer, environmentally sensitive, and the seed of the bletilla striata is very tiny and nothing
Endosperm, therefore be difficult to sprout and grow under natural situation, the cultivation of seedling is more difficult, and traditional cultivation is numerous mainly by plant division
It grows.And the bletilla polysaccharide content that existing method culture obtains is low, quality is not good enough.
Invention content
The object of the present invention is to provide a kind of implantation methods of the good bletilla striata of quality.The method of the present invention is to bletilla striata seeds
When being cultivated, bletilla striata seedling percent is high, and the seed sprouting period is short, and obtained bletilla polysaccharide content is high, and bletilla striata quality is good.
In order to solve the above technical problems, the present invention is realized using following technical scheme：
A kind of implantation methods of the good bletilla striata of quality, include the following steps：
（1）Environment disinfected：Tissue culture operating room, culturing room are sterilized, then HD-850 type table above formula horizontal blast cleanings work
Platform is placed in tissue culture operating room and carries out disinfection, and ensures to be in gnotobasis in workbench workspace；
（2）The preparation of culture medium：The culture medium calculates by weight, mainly by 10-30 parts of ammonium nitrate, calcium chloride dihydrate 1-20
Part, 10-30 parts of epsom salt, 1-20 parts of potassium dihydrogen phosphate, 1-15 parts of potassium iodide, 1-15 parts of boric acid, 100-130 parts of sawdust,
100-110 parts of the cornmeal mush of 90-110 parts of mashed potatoes and a concentration of 80-90% mix；
（3）Seed treatment：The capsule for smearing the bletilla striata after the bletilla striata pollinates with the aqueous solution of auxin, using containing Mycen osmundicola,
Dendrobium mixture, the aqueous solution for opening the small mushroom of lip are sprayed on the mature seed of the bletilla striata；
（4）Sowing：After being sterilized in greenhouse, culturing room carries out illumination using fluorescent lamp, and control temperature is at 20-25 DEG C, humidity 60%
More than, it sprouts within one month, seedling is overstocked after two months and medium bottle nutrition exhausts, and medium bottle is needed to change after 2 months
Sub-bottle is carried out, 3-4 months i.e. bottle outlets of continuation can hardening after replacing medium bottle；
（5）Hardening：Seedling is taken out from culture bottle, with 800 ~ 1000 times of carbendazol soaking and washings 2-3 times, by the training of root
Foster base is washed off, then in plantation to compost seed plate；The seed plate for having planted bletilla striata seedling is put into experienced seedling greenhouse and is cultivated 2-3 months,
Using tidal irrigation；And foliar fertilizer is periodically sprayed, greenhouse is removed after cultivating 2-3 months, moves cultivation two months in the woods to, i.e.,
Obtain bletilla striata seedling；
（6）Selection of land site preparation：Loose fertile sandy loam and humus loam, warm, slightly dark and damp environment is selected to can not resist cold；Row
When the plantation of water good mountainous region, Schattenseite virgin soil is preferably selected to transplant, soil is turned over 20 centimetres or more, manuring and compost are applied per acre
Farm manure 950-1000kg；It paddles by line-spacing 30-50cm；
（7）Field planting：Bletilla striata seedling is taken, by spacing in the rows 12-17cm, line-spacing 18-22cm Furrow sowings, ditch depth 8-10cm, kind rear cover 3-
The fine earth of 4cm thickness；
（8）Greenhouse construction：Plastic film shed is set, and summer canopy both sides need to reserve the gap of 1.5m, cover on a plastic film
The sunshade net of 85% or more shading rate of lid, and cover completely, film is covered bottom by winter, and removes sunshade net in time；
（9）Field management
A. seedling thinning：Seedling thinning is carried out when plant height 6-8cm, each cave stays 2-3 plants, weeds out the rank grass, and shallow hoe 5-8cm is carefully closed lightly and loosened the soil, and
Spray 2-3 S 3307；
1.b. prevents pest and disease damage：It is prevented using Antagonistic Fungi, the Antagonistic Fungi is to be pressed by trichoderma reesei and bacillus subtilis
Weight ratio is 0.5-1.5: 1-2 to mix, and applies 10-15kg per acre.
The implantation methods of the good bletilla striata of quality above-mentioned, the step（2）In, the culture medium calculates by weight, main
It will be by 15-25 parts of ammonium nitrate, 5-15 parts of calcium chloride dihydrate, 15-25 parts of epsom salt, 5-15 parts of potassium dihydrogen phosphate, potassium iodide
5-10 parts, 100-110 parts of the cornmeal mush of 5-10 parts of boric acid, 105-125 parts of sawdust, 95-105 parts of mashed potatoes and a concentration of 80-90%
It mixes.
The implantation methods of the good bletilla striata of quality above-mentioned, the step（2）In, the culture medium calculates by weight, main
It will be by 20 parts of ammonium nitrate, 10 parts of calcium chloride dihydrate, 20 parts of epsom salt, 10 parts of potassium dihydrogen phosphate, 8 parts of potassium iodide, boric acid 8
105 parts of the cornmeal mush of part, 115 parts of sawdust, 100 parts of mashed potatoes and a concentration of 80-90% mixes.
The implantation methods of the good bletilla striata of quality above-mentioned, the farm manure calculate by weight, mainly by ammonium persulfate 5-
10 parts, 1-5 parts of borax, 5-9 parts of microbial manure, 4-9 parts of balsampear root, 7-10 parts of bentonite, wheat bran 9-16
Part, 10-20 parts of chicken manure, 1-10 parts of weathered coal, 1-8 parts of plant ash, 1-10 parts of pig manure, 5-15 parts of land plaster, dregs of fat 1-5
2-4 parts of part, 10-20 parts of albumen mud and potassium are prepared.
The implantation methods of the good bletilla striata of quality above-mentioned, the farm manure calculate by weight, mainly by ammonium persulfate 8
Part, 7 parts of diammonium hydrogen phosphate, 3 parts of borax, 7 parts of microbial manure, 7 parts of balsampear root, 8 parts of bentonite, 12 parts of wheat bran, chicken manure 15
Part, 5 parts of weathered coal, 4 parts of plant ash, 5 parts of pig manure, 10 parts of land plaster, 4 parts of the dregs of fat, 15 parts of albumen mud and 3 parts of preparations of potassium
It forms.
Compared with prior art, when the method for the present invention cultivates bletilla striata seeds, bletilla striata seedling percent is high, and seed is sprouted
The hair period is short, and obtained bletilla polysaccharide content is high, and bletilla striata quality is good.
Specific implementation mode：
Embodiment 1：
A kind of implantation methods of the good bletilla striata of quality, include the following steps：
（1）Environment disinfected：Tissue culture operating room, culturing room are sterilized, then HD-850 type table above formula horizontal blast cleanings work
Platform is placed in tissue culture operating room and carries out disinfection, and ensures to be in gnotobasis in workbench workspace；
（2）The preparation of culture medium：The culture medium is by ammonium nitrate 20kg, calcium chloride dihydrate 10kg, epsom salt 20kg, phosphoric acid
The cornmeal mush 105kg of potassium dihydrogen 10kg, potassium iodide 8kg, boric acid 8kg, sawdust 115kg, mashed potatoes 100kg and a concentration of 80-90%
It mixes；
（3）Seed treatment：The capsule for smearing the bletilla striata after the bletilla striata pollinates with the aqueous solution of auxin, using containing Mycen osmundicola,
Dendrobium mixture, the aqueous solution for opening the small mushroom of lip are sprayed on the mature seed of the bletilla striata；
（4）Sowing：After being sterilized in greenhouse, culturing room carries out illumination using fluorescent lamp, and control temperature is at 20-25 DEG C, humidity 60%
More than, it sprouts within one month, seedling is overstocked after two months and medium bottle nutrition exhausts, and medium bottle is needed to change after 2 months
Sub-bottle is carried out, 3-4 months i.e. bottle outlets of continuation can hardening after replacing medium bottle；
（5）Hardening：Seedling is taken out from culture bottle, with 900 times of carbendazol soaking and washings 3 times, the culture medium of root is washed
Fall, then in plantation to compost seed plate；The seed plate for having planted bletilla striata seedling is put into experienced seedling greenhouse and is cultivated 3 months, using tide
Formula is irrigated；And foliar fertilizer is periodically sprayed, greenhouse is removed after cultivating 2 months, moves in the woods and cultivates two months to get bletilla striata children
Seedling；
（6）Selection of land site preparation：Loose fertile sandy loam and humus loam, warm, slightly dark and damp environment is selected to can not resist cold；Row
When the plantation of water good mountainous region, Schattenseite virgin soil is preferably selected to transplant, soil is turned over 20 centimetres or more, manuring and compost are applied per acre
Farm manure 1000kg；It paddles by line-spacing 30-50cm；
（7）Field planting：Bletilla striata seedling is taken, by spacing in the rows 12-17cm, line-spacing 18-22cm Furrow sowings, ditch depth 8-10cm, kind rear cover 3-
The fine earth of 4cm thickness；
（8）Greenhouse construction：Plastic film shed is set, and summer canopy both sides need to reserve the gap of 1.5m, cover on a plastic film
The sunshade net of 85% or more shading rate of lid, and cover completely, film is covered bottom by winter, and removes sunshade net in time；
（9）Field management
A. seedling thinning：Seedling thinning is carried out when plant height 6-8cm, each cave stays 2-3 plants, weeds out the rank grass, and shallow hoe 5-8cm is carefully closed lightly and loosened the soil, and sprays
It applies and sprays 2-3 S 3307；
B. pest and disease damage is prevented：It is prevented using Antagonistic Fungi, the Antagonistic Fungi is by trichoderma reesei and bacillus subtilis by weight
Amount applies 15kg per acre than being mixed for 1: 1.5.
The farm manure is by ammonium persulfate 8kg, diammonium hydrogen phosphate 7kg, borax 3kg, microbial manure 7kg, balsampear root
7kg, bentonite 8kg, 12 kg of wheat bran, chicken manure 15kg, weathered coal 5kg, 4 kg of plant ash, pig manure 5kg, land plaster 10kg,
Dregs of fat 4kg, albumen mud 15 kg and potassium 3kg are mixed.
Claims (5)
1. a kind of implantation methods of the good bletilla striata of quality, it is characterised in that：Include the following steps：
（1）Environment disinfected：Tissue culture operating room, culturing room are sterilized, then HD-850 type table above formula horizontal blast cleanings work
Platform is placed in tissue culture operating room and carries out disinfection, and ensures to be in gnotobasis in workbench workspace；
（2）The preparation of culture medium：The culture medium calculates by weight, mainly by 10-30 parts of ammonium nitrate, calcium chloride dihydrate 1-20
Part, 10-30 parts of epsom salt, 1-20 parts of potassium dihydrogen phosphate, 1-15 parts of potassium iodide, 1-15 parts of boric acid, 100-130 parts of sawdust,
100-110 parts of the cornmeal mush of 90-110 parts of mashed potatoes and a concentration of 80-90% mix；
（3）Seed treatment：The capsule for smearing the bletilla striata after the bletilla striata pollinates with the aqueous solution of auxin, using containing Mycen osmundicola,
Dendrobium mixture, the aqueous solution for opening the small mushroom of lip are sprayed on the mature seed of the bletilla striata；
（4）Sowing：After being sterilized in greenhouse, culturing room carries out illumination using fluorescent lamp, and control temperature is at 20-25 DEG C, humidity 60%
More than, it sprouts within one month, seedling is overstocked after two months and medium bottle nutrition exhausts, and medium bottle is needed to change after 2 months
Sub-bottle is carried out, 3-4 months i.e. bottle outlets of continuation can hardening after replacing medium bottle；
（5）Hardening：Seedling is taken out from culture bottle, with 800 ~ 1000 times of carbendazol soaking and washings 2-3 times, by the training of root
Foster base is washed off, then in plantation to compost seed plate；The seed plate for having planted bletilla striata seedling is put into experienced seedling greenhouse and is cultivated 2-3 months,
Using tidal irrigation；And foliar fertilizer is periodically sprayed, greenhouse is removed after cultivating 2-3 months, moves cultivation two months in the woods to, i.e.,
Obtain bletilla striata seedling；
（6）Selection of land site preparation：Loose fertile sandy loam and humus loam, warm, slightly dark and damp environment is selected to can not resist cold；Row
When the plantation of water good mountainous region, Schattenseite virgin soil is preferably selected to transplant, soil is turned over 20 centimetres or more, manuring and compost are applied per acre
Farm manure 950-1000kg；It paddles by line-spacing 30-50cm；
（7）Field planting：Bletilla striata seedling is taken, by spacing in the rows 12-17cm, line-spacing 18-22cm Furrow sowings, ditch depth 8-10cm, kind rear cover 3-
The fine earth of 4cm thickness；
（8）Greenhouse construction：Plastic film shed is set, and summer canopy both sides need to reserve the gap of 1.5m, cover on a plastic film
The sunshade net of 85% or more shading rate of lid, and cover completely, film is covered bottom by winter, and removes sunshade net in time；
（9）Field management
A. seedling thinning：Seedling thinning is carried out when plant height 6-8cm, each cave stays 2-3 plants, weeds out the rank grass, and shallow hoe 5-8cm is carefully closed lightly and loosened the soil, and
Spray 2-3 S 3307；
B. pest and disease damage is prevented：It is prevented using Antagonistic Fungi, the Antagonistic Fungi is by trichoderma reesei and bacillus subtilis by weight
Amount applies 10-15kg per acre than being mixed for 0.5-1.5: 1-2.
2. the implantation methods of the good bletilla striata of quality as described in claim 1, it is characterised in that：The step（2）In, the training
Foster base calculates by weight, mainly by 15-25 parts of ammonium nitrate, 5-15 parts of calcium chloride dihydrate, 15-25 parts of epsom salt, phosphoric acid
5-15 parts of potassium dihydrogen, 5-10 parts of potassium iodide, 5-10 parts of boric acid, 105-125 parts of sawdust, 95-105 parts of mashed potatoes and a concentration of 80-
90% 100-110 parts of cornmeal mush mixes.
3. the implantation methods of the good bletilla striata of quality as claimed in claim 2, it is characterised in that：The step（2）In, the training
Foster base calculates by weight, mainly by 20 parts of ammonium nitrate, 10 parts of calcium chloride dihydrate, 20 parts of epsom salt, potassium dihydrogen phosphate 10
105 parts of the cornmeal mush mixing of part, 8 parts of potassium iodide, 8 parts of boric acid, 115 parts of sawdust, 100 parts of mashed potatoes and a concentration of 80-90% and
At.
4. the implantation methods of the good bletilla striata of quality as described in claim 1, it is characterised in that：The farm manure is by weight
It calculates, mainly by 5-10 parts of ammonium persulfate, 1-5 parts of borax, 5-9 parts of microbial manure, 4-9 parts of balsampear root, bentonite
7-10 parts, 9-16 parts of wheat bran, 10-20 parts of chicken manure, 1-10 parts of weathered coal, 1-8 parts of plant ash, 1-10 parts of pig manure, gypsum
2-4 parts of 5-15 parts of powder, 1-5 parts of the dregs of fat, 10-20 parts of albumen mud and potassium are prepared.
5. the implantation methods of the good bletilla striata of quality as claimed in claim 4, it is characterised in that：The farm manure is by weight
It calculates, mainly by 8 parts of ammonium persulfate, 7 parts of diammonium hydrogen phosphate, 3 parts of borax, 7 parts of microbial manure, 7 parts of balsampear root, bentonite 8
Part, 12 parts of wheat bran, 15 parts of chicken manure, 5 parts of weathered coal, 4 parts of plant ash, 5 parts of pig manure, 10 parts of land plaster, 4 parts of the dregs of fat, albumen
3 parts of 15 parts of mud and potassium are prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610959505.XA CN108371008A (en) | 2016-10-27 | 2016-10-27 | A kind of implantation methods of the good bletilla striata of quality |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610959505.XA CN108371008A (en) | 2016-10-27 | 2016-10-27 | A kind of implantation methods of the good bletilla striata of quality |
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| Publication Number | Publication Date |
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| CN108371008A true CN108371008A (en) | 2018-08-07 |
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| CN201610959505.XA Pending CN108371008A (en) | 2016-10-27 | 2016-10-27 | A kind of implantation methods of the good bletilla striata of quality |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207590A (en) * | 2021-04-29 | 2021-08-06 | 南京工业大学大丰海洋产业研究院 | Bletilla direct seeding and seedling raising method utilizing symbiotic bacteria |
| CN113416652A (en) * | 2021-06-25 | 2021-09-21 | 昆明理工大学 | Gastrodia elata seed germination bacterium and application thereof |
-
2016
- 2016-10-27 CN CN201610959505.XA patent/CN108371008A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207590A (en) * | 2021-04-29 | 2021-08-06 | 南京工业大学大丰海洋产业研究院 | Bletilla direct seeding and seedling raising method utilizing symbiotic bacteria |
| CN113416652A (en) * | 2021-06-25 | 2021-09-21 | 昆明理工大学 | Gastrodia elata seed germination bacterium and application thereof |
| CN113416652B (en) * | 2021-06-25 | 2023-05-02 | 昆明理工大学 | Gastrodia elata seed germination strain and application thereof |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180807 |
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