CN108359611A - A kind of rapid conversion scale livestock farming discards the bioconversion method of animal and its tissue - Google Patents
A kind of rapid conversion scale livestock farming discards the bioconversion method of animal and its tissue Download PDFInfo
- Publication number
- CN108359611A CN108359611A CN201810144954.8A CN201810144954A CN108359611A CN 108359611 A CN108359611 A CN 108359611A CN 201810144954 A CN201810144954 A CN 201810144954A CN 108359611 A CN108359611 A CN 108359611A
- Authority
- CN
- China
- Prior art keywords
- animal
- tissue
- discards
- ovum
- bioconversion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/007—Fertilisers made from animal corpses, or parts thereof from derived products of animal origin or their wastes, e.g. leather, dairy products
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/05—Treatments involving invertebrates, e.g. worms, flies or maggots
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/685—Aspergillus niger
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Virology (AREA)
- Pest Control & Pesticides (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Plant Pathology (AREA)
- Insects & Arthropods (AREA)
- Fertilizers (AREA)
- Fodder In General (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses the bioconversion methods that a kind of rapid conversion scale livestock farming discards animal and its tissue, belong to discarded animal and tissue treatment field.It includes:(1) discarded animal and its biochemical precomminution are handled;(2) by after crushing discarded animal and its tissue disintegrating slag carry out enzymolysis processing;(3) special larva ovum processing:Screening, raising domestication amino acid, glucose adapt to larva and collect special larva ovum;(4) in the disintegrating slag special larva ovum being placed in after enzymolysis carry out breeding drosophila;(5) production of worm material separating treatment and drosophila industrialization product.The present invention can it is safe efficient, on the spot, quick resource treatment scale farm discard animal and its tissue, products obtained therefrom is high-end bio-organic fertilizer, insect protein after processing.
Description
Technical field
The invention belongs to discarded animal and its tissue treatment field, more particularly to a kind of rapid conversion scale livestock farming is useless
Abandon the bioconversion method of animal and its tissue.
Background technology
With flourishing for aquaculture, butchers the relevant enterprises such as processing, meat products processing and fur processing and also rapidly send out
Exhibition, such as discarded spoil of resulting attached rubbish, organs and tissues, blood stains, excrement also cause environment huge
Pressure will generate serious problem of environmental pollution if not dealt carefully with, directly affect the production life of surrounding area resident
It is living.
According to《People's Republic of China's animal epidemic prevention method》Regulation:At present can it is innoxious to discarded animal and its tissue,
The method of minimizing and recycling treatment has sanitary landfills, compost and burning.From technical matters angle analysis, each method is pursued
Target it is consistent, but cut both ways, technology complexity differs greatly:
(1) burning method, this method will produce secondary pollution, and highly toxic three cause (carcinogenic, teratogenesis, mutagenesis) class gas danger
Evil health;
(2) high temperature and high pressure method, this method are handled spoil using high-temperature high-pressure apparatus, which belongs to special
Kind equipment, needs specially to be equipped with kettleman, and the waste water and exhaust gas generated needs special equipment reprocessing to can be only achieved environmentally friendly mark
Standard, the product of the method need to be separated by solid-liquid separation, and need to add separation equipment;
(3) sanitary landfills, the method take up a large area, permanent land occupation, discarded animal and its tissue when landfill
Corrupt aerogenesis causes bubble to emerge and fluid seepage, and the chemical substance (quick lime and bleaching powder) that when landfill uses deposits underground water
It in larger security risk, and with seasonal variations, annual summer and autumn in spring, stinks to high heaven, mosquitos and flies grows, and seriously affects week
Side resident's productive life;
(4) traditional fermentation process makes compost fermentation with mixtures such as rice chaff, sawdust, stalk, corncobs, general 1~3
The moon generates completion, and the time cycle is longer, long-term land occupation resource, and fermentation process generates odor pollution and is difficult to control.
(5) cryogenic freezing crush method, Chinese Patent Application No. 201510110372.4,104690075 A of publication number CN are public
It has opened and a kind of spoil has been subjected to cryogenic freezing, broken, then the spoil through pyrolytic, separation of solid and liquid, sterilizing and drying
Method for innocent treatment, although this method realizes recycling for resource, but cost is higher, is unfavorable for industrial application.
(6) microorganism treatment:Chinese Patent Application No. 201510572469.7,105199980 A of publication number CN are disclosed
A kind of method of complex microorganism preparations and harmless treatment spoil, using containing bacillus licheniformis, solution thiamine
Bacillus, the complex microorganism preparations of S. cervisiae and zytase and spoil mixing are fermented;With animal corpse
After body mixing, bacillus licheniformis, bacillus aneurinolytieus, S. cervisiae can mushroom out breeding, metabolism, to generate
Many enzymes;Organic matter decomposition in fermenting bed padding and spoil is metabolized as many small molecule objects by generated enzyme
Matter, these small-molecule substances further promote bacillus licheniformis in complex microorganism, bacillus aneurinolytieus, wine brewing ferment
The growth and breeding of female bacterium, to reach fast degradation spoil.This method can reduce micro- in the corpse for dead livestock and poultry of degrading
The pernicious gases such as the ammonia and organic amine that are discharged during biological decomposition livestock and poultry corpse have safety, high efficiency and nothing
The advantages that side effect.But this method process cycle or long, 15~56 days are needed, and animal corpse after undisclosed degradation
The processing method of body residue and application, if it is unknown to meet following resourceization.
Invention content
1, it to solve the problems, such as
Fail to ask what its bioconversion recycled for microbial degradation spoil and tissue is used in the prior art
Topic, the present invention provide the bioconversion method that a kind of quick treatment scale farm discards animal and its tissue.The present invention adopts
The substances such as macro-molecular protein, starch, the polysaccharide in discarded animal and its tissue are quickly cracked with the enzyme using microorganism secretion,
Then animal and its tissue are discarded using special insect larvae ovum metabolic conversion, production process is pollution-free, near-zero release,
This method can the safe and efficient treatment scale of quick resource on the spot farm discard animal and its tissue, it is final to realize biology
Conversion, obtains high-end bio-organic fertilizer, while can be used for the products such as propagating insects albumen.
2, technical solution
To solve the above problems, the present invention adopts the following technical scheme that.
A kind of rapid conversion scale livestock farming discards the bioconversion method of animal and its tissue, it is characterised in that:Packet
Include following steps:
(1) discarded animal and its biochemical precomminution are handled;
(2) by after step (1) described crushing discarded animal and its tissue disintegrating slag carry out enzymolysis processing;
(3) special larva ovum processing:Screening, raising domestication amino acid, glucose adapt to larva and collect special larva ovum;
(4) in disintegrating slag step (3) the special larva ovum being placed in after step (2) described enzymolysis carry out breeding drosophila;
(5) the worm material after breeding in step (4) is subjected to separating treatment.
Preferably, animal is discarded described in step (1) and its biochemical precomminution processing is:To discarded animal and its group
Be woven at 100~300 DEG C and carry out sterilization treatment, then by after sterilizing discarded animal and its tissue be put into pulverizer, mechanical powder
20~100 mesh are broken to, disintegrating slag enters enzymolysis processing after crushing.The filtrate generated in crushing process enters water treatment unit, uses
Conventional biological oxidation handles such as SCR, A/O, to remove the organic pollution in filtrate, COD, ammonia nitrogen etc. to realize row up to standard
It puts, disintegrating slag enters enzymolysis processing after crushing.
Preferably, the enzymolysis processing described in step (2) is that the enzyme secreted using complex microorganism is quick as enzymolysis agent
Crack discarded animal and its macro-molecular protein, starch, polysaccharide in tissue.
Preferably, complex microbial inoculum described in step (2) includes aspergillus niger, bacillus amyloliquefaciens and solution polysaccharide
Bacillus, and three is according to mass ratio 1:1:The ratio of (1~3) is uniformly mixed.Aspergillus niger viable bacteria amount in every gram of composite bacteria agent
Not less than 1 × 108A, bacillus amyloliquefaciens viable bacteria amount is not less than 1 × 109A, solution polysaccharide bacillus living amount is not less than 1
×108It is a.
Preferably, it is 1 in mass ratio that the enzymolysis agent described in step (2), which is by composite bacteria agent and absorption culture medium,:(2
~5), temperature is 25~30 DEG C, relative humidity is that 60~80%RH carries out mixed culture fermentation, until absorption culture medium is by yellow
Become brown, enzymatic production terminates to obtain enzymolysis agent, enzymolysis agent is mixed with discarded animal and its tissue disintegrating slag, enzymolysis agent
The enzyme of middle secretion quickly cracks the substances such as discarded animal and its macro-molecular protein, starch, polysaccharide in internal organs disintegrating slag, is converted to
Amino acid, glucose etc. are easy to the substance absorbed.
Preferably, the mass ratio of enzymolysis agent described in step (2) and discarded animal and its tissue disintegrating slag is 1:(50~
200), the enzymolysis processing time is 1~3h.
Preferably, the processing of special larva ovum described in step (3) is screened from all kinds of drosophilas, raise domestication amino acid,
Glucose adapts to larva, and carries out pure ovum collecting using pure ovum collecting technology.Its concrete operation step is as follows:It is received using cage system
Collect drosophila ovum, adult housefly is placed in cage, hatching basin dedicated is placed below cage, it is special in hatching using fresh meat mincing and wheat bran
With trapping collection ovum is carried out in basin, drosophila can converge food in matrix, mate, oviposition (in caking shape), the fly ovum of collection
It is preserved for 24 hours under the conditions of 4 DEG C.
Preferably, special larva ovum described in step (4) with after enzymolysis processing discarded animal and its tissue disintegrating slag
Mass ratio is (1~8):5000.
Preferably, the time of the breeding drosophila described in step (4) is 4~5 days.
Preferably, the worm material separating treatment described in step (5) is to detach drosophila larvae and adult flies using worm material seperator
The remaining bottom material of polypide and disintegrating slag after breeding, the raw material by the drying of remaining bottom material as production organic fertilizer, by drosophila larvae
With adult flies drying as protein feed, medicinal raw material.
In the present invention, aspergillus niger, bacillus amyloliquefaciens, solution polysaccharide bacillus are that strain is bought in market.
The present invention also provides a kind of complex microbial inoculum for discarding animal and its tissue for enzymolysis processing, micro- lifes
Object composite bacteria agent includes aspergillus niger, bacillus amyloliquefaciens, solution polysaccharide bacillus, and three is according to mass ratio 1:1:(1~3)
Ratio mixes.
3, advantageous effect
Compared with the prior art, beneficial effects of the present invention are:
(1) enzymolysis processing is carried out to discarded animal and its tissue using the present invention, and by the disintegrating slag after enzymolysis for numerous
Drosophila is grown, discarded spoil is not only avoided and its organizes the environmental pollution that may be brought, and makes discarded animal and its group
It knits and transforms into organic fertilizer, be further used to breed drosophila as protein feed etc., the biology for realizing waste turns
Change, turns waste into wealth, generate certain economic benefit;Primary decrement is (after the quality of raw animal corpse or tissue is dried with processing
As the difference of the quality of organic fertilizer) up to 80%, have the advantage of minimizing;
(2) the middle degradation spoil period needs 15~56 days compared with prior art, and the method for the present invention can be within 1 week
Spoil and tissue are rapidly and efficiently handled, the processing for discarding animal and its tissue is completed, process cycle is short, and processing method is high
Effect;
(3) use the innoxious degree for discarding spoil and tissue at the method for the present invention high, processing procedure is without secondary dirt
Dye, material is odorless after processing;
(4) defective material after discarded animal and its tissue treatment culture is a kind of pest-resistant disease-resistant high level organic fertilizer by the present invention,
Its fertilizer efficiency is higher than composting production, does not grow fly and maggot.
Description of the drawings
Fig. 1 is the bioconversion method technique that a kind of rapid conversion scale livestock farming of the present invention discards animal and its tissue
Flow chart;
Fig. 2 is the process flow chart of the embodiment of the present invention 1 and embodiment 4.
Specific implementation mode
In the complex microorganism preparations that the present invention uses, aspergillus niger viable bacteria amount is not less than 1 × 108It is a/gram, solution starch gemma
Bacillus viable bacteria amount is not less than 1 × 109It is a/gram, solution polysaccharide bacillus living content be not less than 1 × 108A/gram.
The present invention is further described below with reference to specific embodiment.
Embodiment 1
Present invention will now be described in detail with reference to the accompanying drawings..
As shown in Fig. 2, the bioconversion method that rabbit intestines are discarded in a kind of quick treatment scale warren includes the following steps:
(1) rabbit intestines bioconversion pre-processes:100kg rabbits intestines are subjected to sterilization treatment at 200 DEG C, to remove rabbit intestines
In pathogen, then the rabbit intestines after sterilizing are put into pulverizer, mechanical crushing to 100 mesh, what is generated in crushing process is useless
Water enters water treatment unit, and conventional biological oxidation is used to handle SCR techniques to remove the organic pollution in waste water to realize
Qualified discharge obtains 65kg rabbit intestines disintegrating slags and enters enzymolysis processing;
(2) enzymolysis processing:By aspergillus niger, bacillus amyloliquefaciens, solution polysaccharide bacillus according to mass ratio 1:1:1 ratio
Example forms composite bacteria agent after mixing, by composite bacteria agent and absorption culture medium in mass ratio 1:2 carry out mixed culture fermentation,
25 DEG C, 60%RH carries out fermentation production until absorption culture medium becomes brown from yellow, enzymatic production terminates to obtain enzymolysis agent, by enzyme
Solution agent is mixed with rabbit intestines disintegrating slag, and the enzyme secreted in enzymolysis agent quickly cracks macro-molecular protein in rabbit intestines, starch, more
The substances such as sugar are converted to amino acid, glucose etc. and are easy to the substance absorbed, and the mixing quality ratio of enzymolysis agent and rabbit intestines slag is 1:
50, processing time 1h, the rabbit intestines residue 50kg after being digested;
(3) special larva ovum processing:Screening, raising domestication amino acid, glucose adapt to larva, using pure ovum collecting technology
The pure ovum collecting of drosophila is carried out, concrete operation step is as follows:Drosophila ovum is collected using cage system, by drosophila adult (20000)
It is placed in cage, 4 hatching basin dedicateds is placed below cage, it is special in hatching using fresh meat mincing (200 grams) and wheat bran (150 grams)
Trapping collection ovum is carried out in basin, drosophila can converge food in matrix, mate, oviposition (in caking shape), and the fly ovum of collection is 4
It is lower under the conditions of DEG C to preserve for 24 hours.
(4) breeding drosophila processing:It will be bred in rabbit intestines slag that larva ovum is placed in after enzymolysis processing, the matter of the two
Amount is than being 1:5000, it is multiplied into drosophila larvae after 4 days;
(5) separation of worm material and the production of drosophila industrialization product:Drosophila larvae polypide and fruit are detached using worm material seperator
Remaining bottom material after fly growth, remaining bottom material is dried, remaining bottom material 20kg is obtained, as the former material for producing high-end organic fertilizer
Material, drosophila larvae drying, obtains drosophila larvae 5kg, the raw material as protein feed.
Embodiment 2
As shown in Figure 1, a kind of bioconversion method of quick treatment scale pig farm sick dead pig includes the following steps:
(1) sick dead pig bioconversion pre-processes:200kg sick dead pigs are subjected to sterilization treatment at 100 DEG C, to remove sick dead pig
In pathogen, then the sick dead pig after sterilizing is put into pulverizer, mechanical crushing to 20 mesh, the waste water generated in crushing process
Into water treatment unit, conventional biological oxidation processing A/O techniques is used to be reached with the organic pollution removed in waste water to realize
Mark discharge, obtains 140kg sick dead pig disintegrating slags and enters enzymolysis processing;
(2) enzymolysis processing:By aspergillus niger, bacillus amyloliquefaciens, solution polysaccharide bacillus according to mass ratio 1:1:2 ratio
Example forms composite bacteria agent after mixing, by composite bacteria agent and absorption culture medium in mass ratio 1:3 carry out mixed culture fermentation,
27 DEG C, 70%RH carries out fermentation production until absorption culture medium becomes brown from yellow, enzymatic production terminates to obtain enzymolysis agent, by enzyme
Solution agent is mixed with sick dead pig disintegrating slag, and the enzyme secreted in enzymolysis agent quickly cracks macro-molecular protein in sick dead pig, starch, more
The substances such as sugar are converted to amino acid, glucose etc. and are easy to the substance absorbed, and the mixing quality ratio of enzymolysis agent and sick dead pig slag is 1:
200, processing time 3h, the sick dead pig residue 100kg after being digested;
(3) special larva ovum processing:Screening, raising domestication amino acid, glucose adapt to larva, using pure ovum collecting technology
The pure ovum collecting of drosophila is carried out, concrete operation step is as follows:Drosophila ovum is collected using cage system, by drosophila adult (40000)
It is placed in cage, 3 hatching basin dedicateds is placed below cage, it is special in hatching using fresh meat mincing (400 grams) and wheat bran (300 grams)
Trapping collection ovum is carried out in basin, drosophila can converge food in matrix, mate, oviposition (in caking shape), and the fly ovum of collection is 4
It is lower under the conditions of DEG C to preserve for 24 hours.
(4) breeding drosophila processing:It will be bred in sick dead pig slag that larva ovum is placed in after enzymolysis processing, the matter of the two
Amount is than being 3:5000, it is multiplied into drosophila larvae after 4.5 days;
(5) separation of worm material and the production of drosophila industrialization product:Drosophila larvae polypide and fruit are detached using worm material seperator
Remaining bottom material after fly growth, remaining bottom material is dried, remaining bottom material 38kg is obtained, as the former material for producing high-end organic fertilizer
Material, drosophila larvae drying, obtains drosophila larvae 10kg, the raw material as protein feed.
Embodiment 3
As shown in Figure 1, a kind of bioconversion method of quick treatment scale pig farm sick dead pig includes the following steps:
(1) sick dead pig bioconversion pre-processes:1000kg sick dead pigs are subjected to sterilization treatment at 200 DEG C, are died of illness with removal
Pathogen in pig, then the sick dead pig after sterilizing is put into pulverizer, mechanical crushing to 50 mesh, what is generated in crushing process is useless
Water enters water treatment unit, and conventional biological oxidation is used to handle A/O techniques to remove the organic pollution in waste water to realize
Qualified discharge obtains 600kg sick dead pig disintegrating slags and enters enzymolysis processing;
(2) enzymolysis processing:By aspergillus niger, bacillus amyloliquefaciens, solution polysaccharide bacillus according to mass ratio 1:1:3 ratio
Example forms composite bacteria agent after mixing, by composite bacteria agent and absorption culture medium in mass ratio 1:5 carry out mixed culture fermentation,
30 DEG C, 80%RH carries out fermentation production until absorption culture medium becomes brown from yellow, enzymatic production terminates to obtain enzymolysis agent, by enzyme
Solution agent is mixed with sick dead pig disintegrating slag, and the enzyme secreted in enzymolysis agent quickly cracks macro-molecular protein in sick dead pig, starch, more
The substances such as sugar are converted to amino acid, glucose etc. and are easy to the substance absorbed, and the mixing quality ratio of enzymolysis agent and sick dead pig slag is 1:
100, processing time 2h, the sick dead pig residue 450kg after being digested;
(3) special larva ovum processing:Screening, raising domestication amino acid, glucose adapt to larva, using pure ovum collecting technology
The pure ovum collecting of drosophila is carried out, concrete operation step is as follows:Drosophila ovum is collected using cage system, by drosophila adult (200000)
It is placed in cage, 8 hatching basin dedicateds is placed below cage, it is special in hatching using fresh meat mincing (2500 grams) and wheat bran (1500 grams)
With trapping collection ovum is carried out in basin, drosophila can converge food in matrix, mate, oviposition (in caking shape), the fly ovum of collection
Can under the conditions of 4 DEG C under preserve for 24 hours.
(4) breeding drosophila processing:It will be bred in sick dead pig slag that larva ovum is placed in after enzymolysis processing, the matter of the two
Amount is than being 8:5000, it is multiplied into drosophila larvae after 5 days;
(5) separation of worm material and the production of drosophila industrialization product:Drosophila larvae polypide and fruit are detached using worm material seperator
Remaining bottom material after fly growth, remaining bottom material is dried, remaining bottom material 200kg is obtained, as the former material for producing high-end organic fertilizer
Material, drosophila larvae drying, obtains drosophila larvae 60kg, the raw material as protein feed.
Embodiment 4
As shown in Fig. 2, the bioconversion method that rabbit intestines are discarded in a kind of quick treatment scale warren includes the following steps:
(1) rabbit intestines bioconversion pre-processes:1000kg rabbits intestines are subjected to sterilization treatment at 300 DEG C, to remove rabbit intestines
Pathogen in son, then the rabbit intestines after sterilizing are put into pulverizer, mechanical crushing to 50 mesh, what is generated in crushing process is useless
Water enters water treatment unit, and conventional biological oxidation is used to handle SCR techniques to remove the organic pollution in waste water to realize
Qualified discharge obtains 650kg rabbit intestines disintegrating slags and enters enzymolysis processing;
(2) enzymolysis processing:By aspergillus niger, bacillus amyloliquefaciens, solution polysaccharide bacillus according to mass ratio 1:1:1 ratio
Example forms composite bacteria agent after mixing, by composite bacteria agent and absorption culture medium in mass ratio 1:2 carry out mixed culture fermentation,
25 DEG C, 60%RH carries out fermentation production until absorption culture medium becomes brown from yellow, enzymatic production terminates to obtain enzymolysis agent, by enzyme
Solution agent mixed with sick dead pig disintegrating slag, the enzyme secreted in enzymolysis agent quickly crack macro-molecular protein in rabbit intestines slag, starch,
The substances such as polysaccharide are converted to amino acid, glucose etc. and are easy to the substance absorbed, and enzymolysis agent and the mixing quality ratio of rabbit intestines slag are
1:50, processing time 3h, the rabbit intestines residue 500kg after being digested;
(3) special larva ovum processing:Screening, raising domestication amino acid, glucose adapt to larva, using pure ovum collecting technology
The pure ovum collecting of drosophila is carried out, concrete operation step is as follows:Drosophila ovum is collected using cage system, by drosophila adult (500000)
It is placed in cage, 15 hatching basin dedicateds is placed below cage, are being hatched using fresh meat mincing (5000 grams) and wheat bran (3000 grams)
Trapping collection ovum is carried out in basin dedicated, drosophila can converge food in matrix, mate, oviposition (in caking shape), the fly of collection
Ovum preserves for 24 hours under the conditions of 4 DEG C.
(4) breeding drosophila processing:It will be bred in sick dead pig slag that larva ovum is placed in after enzymolysis processing, the matter of the two
Amount is than being 8:5000, it is multiplied into drosophila larvae after 5 days;
(5) separation of worm material and the production of drosophila industrialization product:Drosophila larvae polypide and fruit are detached using worm material seperator
Remaining bottom material after fly growth, remaining bottom material is dried, remaining bottom material 200kg is obtained, as the former material for producing high-end organic fertilizer
Material, drosophila larvae drying, obtains drosophila larvae 60kg, the raw material as protein feed.
Claims (10)
1. a kind of rapid conversion scale livestock farming discards the bioconversion method of animal and its tissue, it is characterised in that:Including
Following steps:
(1) discarded animal and its biochemical precomminution are handled;
(2) by after step (1) described crushing discarded animal and its tissue disintegrating slag carry out enzymolysis processing;
(3) special larva ovum processing:Screening, raising domestication amino acid, glucose adapt to larva and collect special larva ovum;
(4) in disintegrating slag step (3) the special larva ovum being placed in after step (2) described enzymolysis carry out breeding drosophila;
(5) the worm material after breeding in step (4) is subjected to separating treatment.
2. a kind of rapid conversion scale livestock farming according to claim 1 discards the bioconversion side of animal and its tissue
Method, which is characterized in that discard animal described in step (1) and its biochemical precomminution processing is:To discarded animal and its group
Be woven at 100~300 DEG C and carry out sterilization treatment, then by after sterilizing discarded animal and its tissue be put into pulverizer, mechanical powder
20~100 mesh are broken to, disintegrating slag enters enzymolysis processing after crushing.
3. a kind of rapid conversion scale livestock farming according to claim 2 discards the bioconversion side of animal and its tissue
Method, which is characterized in that complex microbial inoculum described in step (2) includes aspergillus niger, bacillus amyloliquefaciens and solution polysaccharide
Bacillus, and three is according to mass ratio 1:1:The ratio of (1~3) is uniformly mixed.
4. a kind of rapid conversion scale livestock farming according to claim 3 discards the bioconversion side of animal and its tissue
Method, which is characterized in that it is 1 in mass ratio that the enzymolysis agent described in step (2), which is by composite bacteria agent and absorption culture medium,:(2~
5), temperature be 25~30 DEG C, relative humidity be 60~80%RH carry out mixed culture fermentation be made.
5. a kind of rapid conversion scale livestock farming according to claim 4 discards the bioconversion side of animal and its tissue
Method, which is characterized in that the mass ratio of enzymolysis agent described in step (2) and discarded animal and its tissue disintegrating slag is 1:(50~200),
The enzymolysis processing time is 1~3h.
6. a kind of rapid conversion scale livestock farming according to claim 5 discards the bioconversion side of animal and its tissue
Method, which is characterized in that the processing of special larva ovum described in step (3) is screened from all kinds of drosophilas, raise domestication amino acid,
Glucose adapts to larva, and carries out pure ovum collecting using pure ovum collecting technology.
7. a kind of rapid conversion scale livestock farming according to claim 6 discards the bioconversion side of animal and its tissue
Method, which is characterized in that the matter of special larva ovum described in step (4) and discarded animal and its tissue disintegrating slag after enzymolysis processing
Amount is than being (1~8):5000.
8. a kind of rapid conversion scale livestock farming according to claim 7 discards the bioconversion side of animal and its tissue
Method, which is characterized in that the time of the breeding drosophila described in step (4) is 4~5 days.
9. a kind of rapid conversion scale livestock farming according to claim 8 discards the bioconversion side of animal and its tissue
Method, which is characterized in that the worm material separating treatment described in step (5) is to detach drosophila larvae and adult flies worm using worm material seperator
The remaining bottom material of body and disintegrating slag after breeding, by remaining bottom material dry as production organic fertilizer raw material, by drosophila larvae with
Adult flies drying is as protein feed, medicinal raw material.
10. a kind of complex microbial inoculum for discarding animal and its tissue for enzymolysis processing, which is characterized in that the microorganism
Composite bacteria agent includes aspergillus niger, bacillus amyloliquefaciens, solution polysaccharide bacillus.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810144954.8A CN108359611B (en) | 2018-02-12 | 2018-02-12 | Biotransformation method for rapidly transforming waste animals and tissues of large-scale farm |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810144954.8A CN108359611B (en) | 2018-02-12 | 2018-02-12 | Biotransformation method for rapidly transforming waste animals and tissues of large-scale farm |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108359611A true CN108359611A (en) | 2018-08-03 |
CN108359611B CN108359611B (en) | 2020-12-18 |
Family
ID=63005613
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810144954.8A Active CN108359611B (en) | 2018-02-12 | 2018-02-12 | Biotransformation method for rapidly transforming waste animals and tissues of large-scale farm |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108359611B (en) |
Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1357514A (en) * | 2000-12-15 | 2002-07-10 | 贵阳医学院 | Method for making organic fertilizer by utilizing housefly larva to ecologically treat pig dung |
WO2010073083A2 (en) * | 2008-12-22 | 2010-07-01 | Inbicon A/S | Methods of processing ensiled biomass |
CN104690075A (en) * | 2015-03-13 | 2015-06-10 | 江西绿环生物科技有限公司 | Carcass innocent treatment method |
CN104984985A (en) * | 2015-08-05 | 2015-10-21 | 江昊 | Infected dead livestock disposal method |
CN105016797A (en) * | 2015-08-07 | 2015-11-04 | 霍英军 | Livestock carcase harmless disposal technology and biological organic fertilizer processing method |
CN105621806A (en) * | 2016-01-22 | 2016-06-01 | 南京贝克特环保科技有限公司 | Biological coagulation oxidation process for rapidly treating kitchen waste wastewater |
CN106984629A (en) * | 2017-04-05 | 2017-07-28 | 上海元始环境科技发展有限公司 | The innoxious decomposing process of animal is carried out using fly maggot |
CN107080042A (en) * | 2017-04-17 | 2017-08-22 | 南昌大学 | The dirty resource utilization method of one kind pig excrement |
CN107200608A (en) * | 2017-04-19 | 2017-09-26 | 南阳师范学院 | A kind of dead livestock and poultry is innoxious, recycling processing method |
KR20170133555A (en) * | 2016-05-25 | 2017-12-06 | 정은희 | Preparation Method of Fertilizer Using Residual Stamping Out Body |
CN107556139A (en) * | 2017-09-29 | 2018-01-09 | 李春华 | A kind of method for innocent treatment for the chicken that dies of illness |
-
2018
- 2018-02-12 CN CN201810144954.8A patent/CN108359611B/en active Active
Patent Citations (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1357514A (en) * | 2000-12-15 | 2002-07-10 | 贵阳医学院 | Method for making organic fertilizer by utilizing housefly larva to ecologically treat pig dung |
WO2010073083A2 (en) * | 2008-12-22 | 2010-07-01 | Inbicon A/S | Methods of processing ensiled biomass |
CN104690075A (en) * | 2015-03-13 | 2015-06-10 | 江西绿环生物科技有限公司 | Carcass innocent treatment method |
CN104984985A (en) * | 2015-08-05 | 2015-10-21 | 江昊 | Infected dead livestock disposal method |
CN105016797A (en) * | 2015-08-07 | 2015-11-04 | 霍英军 | Livestock carcase harmless disposal technology and biological organic fertilizer processing method |
CN105621806A (en) * | 2016-01-22 | 2016-06-01 | 南京贝克特环保科技有限公司 | Biological coagulation oxidation process for rapidly treating kitchen waste wastewater |
KR20170133555A (en) * | 2016-05-25 | 2017-12-06 | 정은희 | Preparation Method of Fertilizer Using Residual Stamping Out Body |
CN106984629A (en) * | 2017-04-05 | 2017-07-28 | 上海元始环境科技发展有限公司 | The innoxious decomposing process of animal is carried out using fly maggot |
CN107080042A (en) * | 2017-04-17 | 2017-08-22 | 南昌大学 | The dirty resource utilization method of one kind pig excrement |
CN107200608A (en) * | 2017-04-19 | 2017-09-26 | 南阳师范学院 | A kind of dead livestock and poultry is innoxious, recycling processing method |
CN107556139A (en) * | 2017-09-29 | 2018-01-09 | 李春华 | A kind of method for innocent treatment for the chicken that dies of illness |
Non-Patent Citations (1)
Title |
---|
韩伟铖等: "规模化猪场废水常规生化处理的效果及原因剖析", 《农业环境科学学报》 * |
Also Published As
Publication number | Publication date |
---|---|
CN108359611B (en) | 2020-12-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN100387551C (en) | Method of producing active nutritional fertilizer using old domostic garbage | |
CN104388363A (en) | Compound bacteria for organic refuse deodorization and reduction and preparation method thereof | |
CN105110833B (en) | Innoxious Rapid Fermentation biological organic fertilizer and preparation method thereof | |
CN109090043A (en) | A kind of black soldier flies larva cultural method | |
CN106365788A (en) | Harmless microorganism treatment method for cadavers of livestock and poultry | |
CN106135884B (en) | Utilize agricultural wastes produced environment friendly function ferment and preparation method thereof | |
CN1919807A (en) | Seaweed organism fertilizer for soil-restored_and environment-improved and preparation method thereof | |
CN107417475A (en) | A kind of agro-ecology fertilizer and preparation method thereof | |
CN101913962A (en) | Process for producing biological organic fertilizer by using garbage | |
CN105255767B (en) | A kind of complex microorganism preparations and fowl fermenting bed padding | |
CN101692788A (en) | Organic biology padding for efficient livestock and poultry and production method thereof | |
CN103396180A (en) | Method for treating animals died from illness by using flammulina velutipes dreg | |
CN105367147A (en) | Harmless processing method for diseased dead pigs | |
CN102391950A (en) | Food waste deodorization composite bacteria and preparation method thereof | |
CN106889030A (en) | A kind of method of utilization dealing with earthworm agricultural organic waste | |
CN109206165A (en) | A kind of method for innocent treatment of sesame duck cultivation field waste | |
KR101553073B1 (en) | Decoposing method for organic sludge | |
CN109170234A (en) | A method of earthworm special feed is prepared using kitchen castoff | |
CN109234349B (en) | Microbial inoculum and method for resource treatment of waste organisms of dead livestock and poultry animals | |
CN108739676A (en) | A kind of method of stalk Treatment and recovery application | |
CN103694009A (en) | Carcass and carcass product innocent treatment method | |
CN108276211A (en) | The biological method of fast and harmless processing dead livestock and poultry under the conditions of low ratio of carbon to ammonium material | |
CN106867941A (en) | A kind of microorganism-decomposing agent for livestock and poultry feces discarded object and its preparation method and application | |
CN104446680A (en) | Wet-process chemical preparation biological transformation method for harmless treatment of dead animals | |
CN1315399C (en) | Forage with swill as primary raw material and its production |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |