CN108354090A - A kind of application method of composite prebiotics composition used for aquiculture - Google Patents

A kind of application method of composite prebiotics composition used for aquiculture Download PDF

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Publication number
CN108354090A
CN108354090A CN201810309732.7A CN201810309732A CN108354090A CN 108354090 A CN108354090 A CN 108354090A CN 201810309732 A CN201810309732 A CN 201810309732A CN 108354090 A CN108354090 A CN 108354090A
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China
Prior art keywords
composite prebiotics
parts
prebiotics composition
dilution
composite
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CN201810309732.7A
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Chinese (zh)
Inventor
金香
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Suzhou Cheng En Fishery Science And Technology Co Ltd
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Suzhou Cheng En Fishery Science And Technology Co Ltd
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Priority to CN201810309732.7A priority Critical patent/CN108354090A/en
Publication of CN108354090A publication Critical patent/CN108354090A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • A23K20/147Polymeric derivatives, e.g. peptides or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)

Abstract

The present invention relates to a kind of application methods of composite prebiotics composition used for aquiculture, it includes the following steps:(a)Composite prebiotics composition is dispensed into multiple plastic bottles, sealing is fermented under conditions of being placed on 28 ~ 37 DEG C makes the plastic bottle swell;(b)Composite prebiotics composition after fermentation is poured into water dilution, the mass ratio of the composite prebiotics composition and water after fermentation is 1:50~100;(c)The dilution is spilled into culture pond and carries out feeding by the usage amount that dilution is determined according to culture pond area, until fishing;Frequency of administration is 1 times/week.Be conducive to inhibit effective bacterium such as vibrios, improve the immunity of aquatic products and plant shoot survival percent, decompose organic impurities and ensure the transparency and finish of water body.

Description

A kind of application method of composite prebiotics composition used for aquiculture
Technical field
The invention belongs to field of fodder, are related to a kind of prebiotic promotor composition, and in particular to a kind of compound benefit used for aquiculture The application method of raw promotor composition.
Background technology
Aquatic products refer to animal and its converted products of ocean and fresh water fishery production.Fresh and living aquatic products, be divided into fish, shrimp, Crab, four major class of shellfish.Aquaculture is the production activity of the lower breeding of artificial control, cultivation and harvest aquatic animals and plants, and aquatic products is supported It is typically to raise the aquatic products seedling of purchase to certain size and sell to grow family.Aquatic products are needed in cultivation using prebiotic Bacterium, vitamin etc. are to improve the resistance of aquatic products, harmful microbe in pool is inhibited to breed.Existing prebiotic promotor composition After being spilled into pool, it is easy to cause the drastically decline of the transparency and finish of water body, this can influence the growth and development of aquatic products.
Invention content
The technical problem to be solved in the invention is one kind compound benefit used for aquiculture proposed in view of the above technical problems The application method of raw promotor composition.
In order to achieve the above objectives, the technical solution adopted in the present invention is:A kind of composite prebiotics combination used for aquiculture The application method of object, it includes the following steps:
(a)Composite prebiotics composition is dispensed into multiple plastic bottles, sealing is fermented under conditions of being placed on 28 ~ 37 DEG C makes institute Plastic bottle is stated to swell;The composite prebiotics composition includes the component of following parts by weight:3 ~ 5 parts of lactic acid bacteria powder, gemma 5 ~ 10 parts of bacillus pulvis, 0.5 ~ 2 part of vitamin E, 10 ~ 15 parts of culture medium, 10 ~ 15 parts of biological raw material, 50 ~ 100 parts of water;
(b)Composite prebiotics composition after fermentation is poured into water dilution, the composite prebiotics composition after fermentation and The mass ratio of water is 1:50~100;
(c)The dilution is spilled into culture pond and carries out feeding by the usage amount that dilution is determined according to culture pond area, until Fishing;Frequency of administration is 1 times/week.
Optimally, the plastic bottle is 500 ~ 1000mL.
Optimally, the fermentation carries out in insulating box.
Since above-mentioned technical proposal is used, the present invention has following advantages compared with prior art:Aquaculture of the present invention With the application method of composite prebiotics composition, is carried out by the composite prebiotics composition of feeding specific components, be conducive to press down Effective bacterium such as vibrios processed improves the immunity of aquatic products and plants shoot survival percent, decomposes organic impurities and ensure the transparent of water body Degree and finish.
Specific implementation mode
The application method of present invention composite prebiotics composition used for aquiculture, it includes the following steps:(a)By compound benefit Raw promotor composition is dispensed into multiple plastic bottles, and sealing is fermented under conditions of being placed on 28 ~ 37 DEG C makes the plastic bottle swell;Institute State the component that composite prebiotics composition includes following parts by weight:3 ~ 5 parts of lactic acid bacteria powder, 5 ~ 10 parts of bacillus pulvis, 0.5 ~ 2 part of vitamin E, 10 ~ 15 parts of culture medium, 10 ~ 15 parts of biological raw material, 50 ~ 100 parts of water;Wherein contain viable count of lactobacillus Amount is about 1 ~ 500,000,000/g, and number of viable bacillus is about 1 ~ 200,000,000/g(Typically bacillus subtilis);Culture medium is bacterium Kind manufacturer or research institution carry out defective material or clout after Spawn incubation, such as culture comprising lactobacillus plantarum and lactobacillus acidophilus Base is using formula(W/V):Molasses 3%, brown sugar 1%, yeast powder 0.6%, peptone 0.5%, KH2PO4 0.2%、CaCO30.5%, So that viable count >=10 after fermentation condition is 28 ~ 37 DEG C of cultures9CFU/ml recycles remaining culture medium;Biological raw material and training Foster base class is seemingly and strain manufacturer or research institution carry out defective material or clout after Spawn incubation, such as comprising husky good fortune bacillus With the nutrient matrix of bacillus pumilus, it is using formula(W/V):Beancake powder 3%, starch 1.5%, corn steep liquor 1%, glucose 0.5%、(NH4)2SO4 0.1%、KH2PO4 0.5%、NaH2PO4 0.5%、MgSO4 0.05%、MnSO40.05%, be in fermentation condition So that viable count >=10 after 28 ~ 37 DEG C of ventilation cultures9CFU/ml recycles remaining nutrient matrix.(b)By the compound benefit after fermentation Raw promotor composition is poured into water dilution, and the mass ratio of the composite prebiotics composition and water after fermentation is 1:50~100;(c) The dilution is spilled into culture pond and carries out feeding by the usage amount that dilution is determined according to culture pond area, until fishing;Hello It is 1 times/week to eat frequency.Be conducive to inhibit effective bacterium such as vibrios, the immunity for improving aquatic products and kind shoot survival percent in this way, divide Solution organic impurities and the transparency and finish for ensureing water body.Plastic bottle is 500 ~ 1000mL, and fermentation carries out in insulating box, this Sample takes convenient for composite prebiotics composition and accurately ensures dosage.
The preferred embodiment of the invention will be described in detail below.
Embodiment 1
The present embodiment provides a kind of application methods of composite prebiotics composition used for aquiculture, it includes the following steps:
(a)Composite prebiotics composition is dispensed into multiple plastic bottles(Conventional Drinking bottle, 500ml), sealing is placed on 28 ~ Fermentation makes plastic bottle swell under conditions of 37 DEG C(As long as plastic bottle does not rupture);Composite prebiotics composition includes following heavy Measure the component of number:3 parts of lactic acid bacteria powder, 10 parts of bacillus pulvis, 2 parts of vitamin E, 10 parts of culture medium, biological raw material 10 parts, 100 parts of water, wherein being about 1 ~ 500,000,000/g containing viable lactic acid bacteria quantity, bacillus subtilis number of viable is about 1 ~ 2 Hundred million/g;Culture medium is the culture medium defective material for cultivating lactobacillus plantarum and lactobacillus acidophilus(It is using formula(W/V):Molasses 3%, Brown sugar 1%, yeast powder 0.6%, peptone 0.5%, KH2PO4 0.2%、CaCO30.5%, after fermentation condition is 28 ~ 37 DEG C of cultures Make viable count >=109CFU/ml);Biological raw material is the nutrient matrix defective material of culture husky good fortune bacillus and bacillus pumilus (It is using formula(W/V):Beancake powder 3%, starch 1.5%, corn steep liquor 1%, glucose 0.5%, (NH4)2SO4 0.1%、KH2PO4 0.5%、NaH2PO4 0.5%、MgSO4 0.05%、MnSO40.05%, so that viable bacteria after fermentation condition is 28 ~ 37 DEG C of ventilation cultures Number >=109CFU/ml);
(b)Composite prebiotics composition after fermentation is poured into water dilution, the composite prebiotics composition and water after fermentation Mass ratio is 1:50;
(c)The usage amount of dilution is determined according to culture pond area(Typically 1 mu of culture pond is with 50 ~ 100 bottles), dilution is spilt Enter and carry out feeding in culture pond, until fishing;Frequency of administration is 1 times/week.
Embodiment 2
The present embodiment provides a kind of application method of composite prebiotics composition used for aquiculture, it with it is basic in embodiment 1 Unanimously, unlike:Step(a)In, composite prebiotics composition includes the component of following parts by weight:Lactic acid bacteria powder 5 Part, 5 parts of bacillus pulvis, 0.5 part of vitamin E, 15 parts of culture medium, 15 parts of biological raw material, 50 parts of water.
Embodiment 3
The present embodiment provides a kind of application method of composite prebiotics composition used for aquiculture, it with it is basic in embodiment 1 Unanimously, unlike:It includes the component of following parts by weight:4 parts of lactic acid bacteria powder, 6 parts of bacillus pulvis, vitamin E 1.5 parts, 13 parts of culture medium, 13 parts of biological raw material, 80 parts of water.
Comparative example 1
This example provides a kind of application method of composite prebiotics composition used for aquiculture, it with it is almost the same in embodiment 1, Unlike:It does not ferment after each raw material mixing.
5 foster shrimp units that shrimp pool is divided into 5m × 5m are rested, the shrimp seedling of equivalent is introduced(Specification is almost the same, ensures Cultivation density), the composite prebiotics composition that is spilled into respectively in the above-mentioned different examples of equivalent(It is one times/week, about prebiotic with 1 kilogram Promotor composition is spilled into after dilutable water;Another is the blank sample for being added without composite prebiotics composition), using identical dragon Shrimp feed is fed(Feed is commercially available, and rearing conditions are almost the same)January;When being spilled into composite prebiotics composition, it can be found that making Become slightly yellow with the foster shrimp unit water colour of composite prebiotics composition in embodiment 1 to embodiment 3, but still keeps higher transparent Degree and finish;Foster shrimp unit water colour using composite prebiotics composition in comparative example 1 becomes more yellow, transparency and finish It is decreased obviously, using flashlight irradiation it can be seen that yellow particle;Use composite prebiotics composition in embodiment 1 to embodiment 3 Foster shrimp unit in lobster head be significantly greater than in comparative example 1, and have no dead shrimp(I.e. survival rate is higher than in comparative example 1), And in comparative example 2 have more dead shrimp and survival rate is relatively low and head is minimum.
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art Scholar cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention Equivalent change or modification made by Spirit Essence, should be covered by the protection scope of the present invention.

Claims (3)

1. a kind of application method of composite prebiotics composition used for aquiculture, which is characterized in that it includes the following steps:
(a)Composite prebiotics composition is dispensed into multiple plastic bottles, sealing is fermented under conditions of being placed on 28 ~ 37 DEG C makes institute Plastic bottle is stated to swell;The composite prebiotics composition includes the component of following parts by weight:3 ~ 5 parts of lactic acid bacteria powder, gemma 5 ~ 10 parts of bacillus pulvis, 0.5 ~ 2 part of vitamin E, 10 ~ 15 parts of culture medium, 10 ~ 15 parts of biological raw material, 50 ~ 100 parts of water;
(b)Composite prebiotics composition after fermentation is poured into water dilution, the composite prebiotics composition after fermentation and The mass ratio of water is 1:50~100;
(c)The dilution is spilled into culture pond and carries out feeding by the usage amount that dilution is determined according to culture pond area, until Fishing;Frequency of administration is 1 times/week.
2. the application method of composite prebiotics composition used for aquiculture according to claim 1, it is characterised in that:The modeling Material bottle is 500 ~ 1000mL.
3. the application method of composite prebiotics composition used for aquiculture according to claim 1, it is characterised in that:The hair Ferment carries out in insulating box.
CN201810309732.7A 2018-04-09 2018-04-09 A kind of application method of composite prebiotics composition used for aquiculture Pending CN108354090A (en)

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CN201810309732.7A CN108354090A (en) 2018-04-09 2018-04-09 A kind of application method of composite prebiotics composition used for aquiculture

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CN108354090A true CN108354090A (en) 2018-08-03

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082345A (en) * 2014-07-24 2014-10-08 中国水产科学研究院珠江水产研究所 Special EM (Effective Microorganisms) bacteria powder for aquaculture and preparation method and application of special EM (Effective Microorganisms) bacteria powder
CN105347511A (en) * 2015-11-30 2016-02-24 威海地宝生物科技有限公司 Compound microbial preparation for aquaculture and method
KR20170082484A (en) * 2017-07-03 2017-07-14 주식회사 이뮤너스 Lactobacillus plantarum fgl0001 and method for enhancing immunity of fish using it

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104082345A (en) * 2014-07-24 2014-10-08 中国水产科学研究院珠江水产研究所 Special EM (Effective Microorganisms) bacteria powder for aquaculture and preparation method and application of special EM (Effective Microorganisms) bacteria powder
CN105347511A (en) * 2015-11-30 2016-02-24 威海地宝生物科技有限公司 Compound microbial preparation for aquaculture and method
KR20170082484A (en) * 2017-07-03 2017-07-14 주식회사 이뮤너스 Lactobacillus plantarum fgl0001 and method for enhancing immunity of fish using it

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Application publication date: 20180803