CN108314495A - A kind of humulus grass zymotic fluid and preparation method thereof that can substitute mineral nutrition - Google Patents
A kind of humulus grass zymotic fluid and preparation method thereof that can substitute mineral nutrition Download PDFInfo
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- CN108314495A CN108314495A CN201810082738.5A CN201810082738A CN108314495A CN 108314495 A CN108314495 A CN 108314495A CN 201810082738 A CN201810082738 A CN 201810082738A CN 108314495 A CN108314495 A CN 108314495A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a kind of humulus grass zymotic fluids that can substitute mineral nutrition, it is prepared by the raw material of following parts by weight：8~10 parts of humulus grass cauline leaf, 2~4 parts of brown sugar, 0.03~0.05 part of dregs of beans, feldspar in powder（Containing K2The mesh of O 8%~10%, grain size >=300）0.01~0.03 part, ground phosphate rock（Containing P2O510%~35%, the mesh of grain size >=300）0.01~0.03 part, 44~52 parts of water.A kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, includes the following steps：（1）Humulus grass material guillotine cutting；（2）It is prepared by brown sugar water；（3）Fermented material mixes；（4）Aerobic fermentation；（5）Anaerobic fermentation；（6）Dilution, filtering.Quality, the use for reducing chemical fertilizer and low manufacture cost, the preparation process that the humulus grass fermentation liquid energy that the present invention makes significantly improves green vegetable are simple, are suitable for large-scale industrial production.
Description
Technical field
The invention belongs to plant soil-less culturing technical field, more particularly to a kind of humulus grass fermentation that can substitute mineral nutrition
Liquid and preparation method thereof.
Background technology
With the improvement of people ' s living standards, the enhancing of environmental consciousness, organic agriculture are developed rapidly, soilless culture
As a kind of advanced cultivation technique, the research of organic nutrient solution for cultivating is affected by the attention of people.Using farming and animal husbandry livestock and poultry
Excrement or processing of farm products byproduct prepare organic nutrient solution for cultivating such as chicken manure, dregs of beans, Rapeseed Meal by Aspergillus Fermentation leaching liquor and carry out romaine lettuce, dish
The heart, water spinach cultivation, achieve good effect.The residuum using natural plant material or industrial crops is had been reported that, with sugar, ferment
Deng for auxiliary material, it is fermented made of plant source organic nutrient solution for cultivating have and promote plant strain growth, enhancing crop root vigor, improve fruit
Real quality inhibits disease and controls the effect of disease, and Sherri A.Miller etc. point out the plants such as bamboo, argy wormwood, Cucurbita
Spray is suitable for fermenting plant nutrient liquor.The cloudy moisture-proof of humulus grass happiness, drought-resistant, barren-resistant, vitality is extremely strong, adapts to a variety of lifes
State environment is a kind of one of important ground cover plant of wild plant and desertification treatment that distribution is very wide.Forefathers research shows that
It is micro containing a variety of inorganic elements such as multiple nutritional components and N P and K and iron etc. such as protein, amino acid, vitamins in humulus grass
Element；Also contain the ingredient of a variety of effects such as anti-inflammatory, antibacterial, anti-oxidant, the country has scholar to be applied to animal feeding, medicine
Clinic also studies have reported that its leaching liquor is to plant seed germination and growth of seedling facilitation, but humulus grass zymotic fluid is made
For organic nutrient solution for cultivating or foliar fertilizer carry out vegetable aquaculture cultivation research and application there is not yet report.
Invention content
The object of the present invention is to provide a kind of humulus grass zymotic fluids and preparation method thereof that can substitute mineral nutrition, to make up
Humulus grass zymotic fluid carries out the research blank of vegetable aquaculture cultivation as organic nutrient solution for cultivating, organic foliar fertilizer.
The purpose of the present invention is what is realized with following technical proposals：
A kind of humulus grass zymotic fluid that can substitute mineral nutrition, it is prepared by the raw material of following parts by weight：Humulus grass cauline leaf 5
~7 parts, 4~6 parts of brown sugar, 0.05~0.07 part of dregs of beans, 0.01~0.03 part of feldspar in powder, 0.01~0.03 part of ground phosphate rock, water
50~60 parts.
Preferably, a kind of humulus grass zymotic fluid that can substitute mineral nutrition, it be by following parts by weight raw material prepare and
At：6 parts of humulus grass cauline leaf, 5 parts of brown sugar, 0.06 part of dregs of beans, 0.02 part of feldspar in powder, 0.02 part of ground phosphate rock, 55 parts of water.
Preferably, the length of a kind of humulus grass zymotic fluid that can substitute mineral nutrition, the humulus grass cauline leaf is 2~3cm.
Preferably, a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, includes the following steps：
（1）Humulus grass material guillotine cutting；
（2）It is prepared by brown sugar water；
（3）Fermented material mixes；
（4）Aerobic fermentation；
（5）Anaerobic fermentation；
（6）Dilution, filtering.
Preferably, a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, step（2）In take one in raw material
Part water, is increased to 25~30 DEG C by water temperature, is put into brown sugar, and brown sugar is completely dissolved obtained brown sugar water.
Preferably, a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, step（3）It is middle first by dregs of beans with
Humulus grass material is sufficiently mixed, and remaining water in raw material, side then will be added in the brown sugar water prepared, feldspar in powder and ground phosphate rock
Side is poured into be stirred.
Preferably, a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, step（4）In stir within every 3~5 days
It mixes 1 time, the fermentation phase is 35 days.
Preferably, a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, step（5）The middle fermentation phase is 15
It.
Preferably, a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, step（6）It is middle total using raw material
The water that 4 times of weight is diluted, using >=40 the polished standard screens, natural filtration.
Beneficial effects of the present invention are as follows：
1, the humulus grass zymotic fluid of preparation method preparation of the present invention is added in improveing Huo Gelan nutrient solutions to aboveground vegetation part
Growth have and slow down effect, have facilitation to the growth of foot end, especially promote the dry matter weight of hypogeal parts of plant
Accumulation, moreover it is possible to significantly improve chlorophyll content and anthocyanidin content, the soluble sugar content and Vc contents of plant.
2, the humulus grass zymotic fluid for preparing the preparation method of the present invention is used as foliar fertilizer, can promote the growth of romaine lettuce overground part
With the elongation of underground part root, the root activity and its anthocyanidin, soluble protein, soluble sugar that can especially significantly improve romaine lettuce contain
Amount and Vc contents.
3, preparation method is simple by the present invention, raw materials used to be also easy to obtain, low manufacture cost, preparation process letter
It is single, it is suitable for large-scale industrial production.
Description of the drawings：
Fig. 1 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce plant height；
Fig. 2 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce stem thickness；
Fig. 3 is influence data statistics figure of the zymotic fluid of different proportion to the romaine lettuce number of blade；
Fig. 4 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce ground fresh weight；
Fig. 5 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce ground dry weight；
Fig. 6 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce underground fresh weight；
Fig. 7 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce underground dry weight；
Fig. 8 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce chlorophyll content；
Fig. 9 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce anthocyanidin content；
Figure 10 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce soluble sugar content；
Figure 11 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce Vc contents；
Figure 12 is influence data statistics figure of the zymotic fluid of different proportion to romaine lettuce soluble protein；
Figure 13 is that the zymotic fluid of different extension rates makees influence data statistics figure of the foliar fertilizer to romaine lettuce chlorophyll relative content；
Figure 14 is that the zymotic fluid of different extension rates makees influence data statistics figure of the foliar fertilizer to romaine lettuce root activity；
Figure 15 is the influence data statistics figure that the zymotic fluid of different extension rates makees foliar fertilizer to nitrogen relative amount in romaine lettuce blade；
Figure 16 is that the zymotic fluid of different extension rates makees influence data statistics figure of the foliar fertilizer to romaine lettuce anthocyanidin relative amount；
Figure 17 is that the zymotic fluid of different extension rates makees influence data statistics figure of the foliar fertilizer to romaine lettuce soluble protein content；
Figure 18 is that the zymotic fluid of different extension rates makees influence data statistics figure of the foliar fertilizer to romaine lettuce nitrate nitrogen content；
Figure 19 is that the zymotic fluid of different extension rates makees influence data statistics figure of the foliar fertilizer to romaine lettuce soluble sugar content；
Figure 20 is that the zymotic fluid of different extension rates makees influence data statistics figure of the foliar fertilizer to romaine lettuce Vc contents.
Specific implementation mode
Embodiment 1
A kind of humulus grass zymotic fluid that can substitute mineral nutrition, it is prepared by the raw material of following parts by weight：Humulus grass cauline leaf 5
~7 parts, 4~6 parts of brown sugar, 0.05~0.07 part of dregs of beans, 0.01~0.03 part of feldspar in powder, 0.01~0.03 part of ground phosphate rock, water
50~60 parts.
A kind of preparation method of humulus grass zymotic fluid that can substitute mineral nutrition, includes the following steps：
（1）Humulus grass material guillotine cutting；
（2）It is prepared by brown sugar water；
（3）Fermented material mixes；
（4）Aerobic fermentation；
（5）Anaerobic fermentation；
（6）Dilution, filtering.
Embodiment 2
A kind of humulus grass zymotic fluid that can substitute mineral nutrition, it is prepared by the raw material of following parts by weight：Humulus grass cauline leaf 6
Part, 5 parts of brown sugar, 0.06 part of dregs of beans, 0.02 part of feldspar in powder, 0.02 part of ground phosphate rock, 55 parts of water.
A kind of preparation method of humulus grass zymotic fluid that can substitute mineral nutrition, includes the following steps：
（1）Humulus grass material guillotine cutting；
（2）It is prepared by brown sugar water：A part of water in raw material is taken, water temperature is increased to 25~30 DEG C, is put into brown sugar, brown sugar is completely molten
Brown sugar water is made in solution；
（3）Fermented material mixes：First dregs of beans and humulus grass material are sufficiently mixed, then by the brown sugar water prepared, feldspar in powder
With remaining water in addition raw material in ground phosphate rock, it is stirred when pouring into；
（4）Aerobic fermentation：It stirs 1 time within every 3~5 days, the fermentation phase is 35 days；
（5）Anaerobic fermentation：The fermentation phase is 15 days；
（6）Dilution, filtering, are diluted using 4 times of water of raw material gross weight, using 40 mesh（Aperture 0.45mm）Standard screen,
Natural filtration.
Embodiment 3
A kind of humulus grass zymotic fluid that can substitute mineral nutrition, it is prepared by the raw material of following parts by weight：Humulus grass cauline leaf 5
Part, 4 parts of brown sugar, 0.05 part of dregs of beans, 0.01 part of feldspar in powder, 0.01 part of ground phosphate rock, 50 parts of water.The length of the humulus grass cauline leaf is
2cm。
A kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, includes the following steps：
（1）Humulus grass material guillotine cutting；
（2）It is prepared by brown sugar water：A part of water in raw material is taken, water temperature is increased to 25~30 DEG C, is put into brown sugar, brown sugar is completely molten
Brown sugar water is made in solution；
（3）Fermented material mixes：First dregs of beans and humulus grass material are sufficiently mixed, then by the brown sugar water prepared, feldspar in powder
With remaining water in addition raw material in ground phosphate rock, it is stirred when pouring into；
（4）Aerobic fermentation：It stirs 1 time within every 3 days, the fermentation phase is 35 days；
（5）Anaerobic fermentation：The fermentation phase is 15 days；
（6）Dilution, filtering, are diluted using 4 times of water of raw material gross weight, using 40 mesh（Aperture 0.45mm）Standard screen,
Natural filtration.
Embodiment 4
A kind of humulus grass zymotic fluid that can substitute mineral nutrition, it is prepared by the raw material of following parts by weight：Humulus grass cauline leaf 7
Part, 6 parts of brown sugar, 0.07 part of dregs of beans, 0.03 part of feldspar in powder, 0.03 part of ground phosphate rock, 60 parts of water.The length of the humulus grass cauline leaf is
3cm。
A kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition, includes the following steps：
（1）Humulus grass material guillotine cutting；
（2）It is prepared by brown sugar water：A part of water in raw material is taken, water temperature is increased to 25~30 DEG C, is put into brown sugar, brown sugar is completely molten
Brown sugar water is made in solution；
（3）Fermented material mixes：First dregs of beans and humulus grass material are sufficiently mixed, then by the brown sugar water prepared, feldspar in powder
With remaining water in addition raw material in ground phosphate rock, it is stirred when pouring into；
（4）Aerobic fermentation：It stirs 1 time within every 3~5 days, the fermentation phase is 35 days；
（5）Anaerobic fermentation, fermentation phase are 20 days；
（6）Dilution, filtering, are diluted using 4 times of water of total weight, using 40 mesh（Aperture 0.45mm）Standard screen, normal pressure
Filtering.
Influence experiment one of the humulus grass zymotic fluid to Growth of Lettuce and quality
1, test objective
This experiment substitutes mineral fertilizer using humulus grass zymotic fluid and carries out romaine lettuce soilless culture, and research humulus grass zymotic fluid is as organic battalion
Influence of the nutrient solution to water planting Growth of Lettuce and its nutritional quality, to for humulus grass comprehensive exploitation and plant source organic nutrient solution for cultivating
Exploitation provides Technical Reference and theoretical foundation with application.
2, test material
The influence experiment of Growth of Lettuce and quality, humulus are carried out using humulus grass zymotic fluid prepared by the method in the embodiment of the present invention 2
The ingredient of careless zymotic fluid is as follows：Full nitrogen 167.38mg/L, full phosphorus 96.32mg/L, full potassium 175.96mg/L, soluble sugar
147.14mg/L soluble solid 0.6%.Romaine lettuce kind is " Italian romaine lettuce ", is bred with Yi Feng standing grain seeds company；Experiment
Using improvement Huo Gelan（Hogagland ＆ Apnon, 1938）Formula nutritional formula of liquid.
3, test period, place
Field trial is carried out 17 days~April 28 March in 2017 in three area's energy saving sunlight greenhouse of Agricultural University Of He'nan；Experiment
Room index test experiment is carried out 29 days~April 30 April in 2017 in College of Horticulture of Agricultural University Of He'nan laboratory.
4, test method
Using completely randomized experiment, five kinds of nutrient solutions of A, B, C, D and CK are set altogether：
A：25%（V/V）Embodiment 2 prepare humulus grass zymotic fluid dilute again the zymotic fluid+75% after 4 times improvement Huo Gelan nutrient solutions；
B：50%（V/V）Embodiment 2 prepare humulus grass zymotic fluid dilute again the zymotic fluid+50% after 4 times improvement Huo Gelan nutrient solutions；
C：75%（V/V）Embodiment 2 prepare humulus grass zymotic fluid dilute again the zymotic fluid+25% after 4 times improvement Huo Gelan nutrient solutions；
D：100% embodiment 2 prepares humulus grass zymotic fluid and dilutes the zymotic fluid after 4 times again；
CK：100% improvement Huo Gelan nutrient solutions.
By the way of hole plate seedling growth, seed is directly multicast in 50 hole hole trays, cave depth 1cm；It is divided into seedlings after 32 days, by seedling
Clear water is washed after root and is placed in customization cup with sponge block package, then in shady place slow seedling 2 days；By the seedling delayed, field planting is arrived at random
In NFT water planting slots；Property of throughout the growing season breaking is for nutrient solution, and daily 7:00-19:00 per hour for nutrient solution 15min, and 20:00-
6:00 respectively at 24:00、6:00 each feed flow 15min；30 plants are one group, and one shares 3 groups, every group of grab sample 6 after field planting 40 days
Strain carries out the acquisition of related data.
5, project and assay method are measured
Measure the item is as follows：Plant height（Basal part of stem is to romaine lettuce plant strain growth point）, base portion stem thickness（Basal part of stem to cotyledon interposition
It sets）, overground part and underground part dry weight and fresh weight of plant seedlings, fresh leaf inner chlorophyll, soluble sugar, soluble protein, Vc, anthocyanidin content.
Measurement method is as follows：Plant height is surveyed with tape measure（Unit：cm）；Base portion stem thickness (unit is surveyed with electronic digital indicator：
mm)；With on assay balance geodetic, underground part dry weight and fresh weight of plant seedlings（Fresh weight is directly measured with balance, dry weight：105 DEG C of 15min that finish, 85 DEG C
Constant temperature is weighed after drying to constant weight）；Spectrophotometry surveys chlorophyll content；Anthrone method surveys soluble sugar content；Coomassie brilliant blue contaminates
Color method surveys soluble protein content；2,6-D titrations survey Vc contents；Absorbance method surveys anthocyanidin content.
All test datas are all made of DPS7.05 softwares and carry out variance analysis, with 2016 softwares of WPS office into line number
According to statistics, it maps, and with LSD methods of inspection to significant difference (P<0.05) Multiple range test is carried out.
6, test result
Shown in Fig. 1-3, plant height, stem thickness, the number of blade of romaine lettuce are all successively decreased with the increase of humulus grass zymotic fluid substitution ratio；For A,
B, the romaine lettuce plant height of C and D nutrient solutions is substantially less than the romaine lettuce plant height for CK nutrient solutions；For the stem thicknesses of A nutrient solutions, the number of blade with
Contrast difference is not notable, and the romaine lettuce stem thickness for CK nutrient solutions is substantially less than for the romaine lettuce stem thickness and the number of blade of B, C and D nutrient solution
And the number of blade.Statistics indicate that the humulus grass zymotic fluid of high concentration, which has the growth of romaine lettuce, slows down effect, A nutrient solutions slow down effect
Minimum, plant height reduce 13.36% compared with control, and stem thickness and the number of blade and contrast difference be not notable.
Shown in Figure 4 and 5, with the increase of humulus grass zymotic fluid substitution ratio, the fresh weight and dry weight of romaine lettuce overground part are all in
The trend successively decreased, fresh weight and dry weight for the romaine lettuce overground part of A, B, C and D nutrient solution are significantly lower than the life for CK nutrient solutions
The fresh weight and dry weight on vegetable plot top, wherein the overground part fresh weight and dry weight for the romaine lettuce of A nutrient solutions are respectively than for CK nutrient solutions
Romaine lettuce overground part fresh weight and dry weight reduce 21.27% and 16.26%.
As shown in Figure 6, the growth that romaine lettuce underground part is significantly inhibited for D nutrient solutions promotes romaine lettuce for A, B and C nutrition liquid energy
The growth of underground part, and it is significantly higher than the romaine lettuce underground part fresh weight for CK nutrient solutions for the romaine lettuce underground part fresh weight of A nutrient solutions.
As shown in Figure 7, the romaine lettuce underground for CK nutrient solutions is significantly higher than for the romaine lettuce underground part dry weight of A, B and C nutrient solution
Portion's dry weight, the romaine lettuce underground part dry weight for D nutrient solutions and the romaine lettuce underground part dry weight difference for CK nutrient solutions is not notable.Tables of data
Bright, humulus grass zymotic fluid, which has the growth of romaine lettuce overground part, slows down effect, and humulus grass is fermented, and liquid proportional is bigger to slow down Growth of Lettuce
Effect is stronger；Humulus grass zymotic fluid promotes the growth of romaine lettuce underground part with mineral nutrition cooperation, especially promotes romaine lettuce underground part dry
The accumulation of matter.
As shown in Figure 8, for the romaine lettuce of A, B, C and D nutrient solution, with the increase of humulus grass zymotic fluid substitution ratio, Chinese leaf
Piece chlorophyll content tapers off trend, but is significantly higher than for CK nutrient solutions for the romaine lettuce chlorophyll content in leaf blades of A and B nutrient solutions
Romaine lettuce chlorophyll content in leaf blades.
As shown in Figure 9, for the romaine lettuce of A, B, C and D nutrient solution, with the increase of humulus grass zymotic fluid substitution ratio, Chinese leaf
Piece anthocyanidin content is in increasing trend, and is all remarkably higher than for CK nutrition for the Chinese leaf piece anthocyanidin content of B, C and D nutrient solution
The Chinese leaf piece anthocyanidin content of liquid.Statistics indicate that a certain proportion of humulus grass fermentation liquid energy promote romaine lettuce blade Determination of Chlorophyll and
The accumulation of anthocyanidin, wherein when for A nutrient solutions romaine lettuce chlorophyll content highest, than the romaine lettuce chlorophyll content for CK nutrient solutions
It is about high by 22.05%, it is best to the facilitation effect of romaine lettuce anthocyanin accumulation when for D nutrient solutions.
As shown in Figure 10, the soluble sugar content of romaine lettuce increases with the increase of humulus grass zymotic fluid substitution ratio, and with confession
The romaine lettuce of CK nutrient solutions reaches the level of signifiance compared to difference, is sought for CK for the romaine lettuce soluble sugar content ratio of A, B, C and D nutrient solution
The romaine lettuce soluble sugar content of nutrient solution is about high by 1.5%, 3.9%, 4.84%, 5.6% respectively, and wherein D nutrient solutions are solvable to promoting romaine lettuce
The effect of property sugar accumulation is best.
As shown in Figure 11, the romaine lettuce for A, B, C and D nutrient solution compared with for the romaine lettuce of CK nutrient solutions can dramatically increase life
The Vc contents of dish, have increased separately 37.8%, 47.9%, 57.98%, 31.93%, are made to the promotion that romaine lettuce Vc is synthesized with C nutrient solutions
With best.
As shown in Figure 12, with the increase of humulus grass zymotic fluid substitution ratio, after the content of soluble protein is in first increasing in romaine lettuce
The trend subtracted, and the soluble protein content of A, B, C and D nutrient solution and the difference of CK nutrient solutions reach the level of signifiance, wherein for A
The romaine lettuce soluble protein content of nutrient solution is significantly higher than the romaine lettuce soluble protein content for CK nutrient solutions, about higher than compareing
13.58%。
7, conclusion (of pressure testing)
This experiment substitutes the Huo Gelan formula nutritional liquid of improvement using the humulus grass zymotic fluid of different proportion, has studied it and is given birth to water planting
Dish is grown and the influence of nutritional quality, test result show：
（1）Humulus grass zymotic fluid, which has the growth of romaine lettuce overground part, slows down effect, and the humulus grass fermentation liquid proportional the big more is given birth to romaine lettuce
Long to slow down effect stronger, uses 25%（V/V）Embodiment 2 prepare humulus grass zymotic fluid dilute again the zymotic fluid+75% after 4 times improvement
The romaine lettuce overground part fresh weight of Huo Gelan nutrient solutions reduces 16.26% compared with the romaine lettuce yield for the improvement Huo Gelan nutrient solutions for using 100%；
Humulus grass zymotic fluid promotes the growth of romaine lettuce underground part with mineral nutrition cooperation, especially promotes the accumulation of romaine lettuce dry matter weight of hypogeal parts.
（2）A certain proportion of humulus grass fermentation liquid energy promotes the generation of romaine lettuce blade Determination of Chlorophyll, uses 25%（V/V）Reality
Apply example 2 prepare humulus grass zymotic fluid dilute again the improvement Huo Gelan nutrient solutions of the zymotic fluid+75% after 4 times romaine lettuce chlorophyll content it is aobvious
It writes high by 22.05% higher than the romaine lettuce of the improvement Huo Gelan nutrient solutions using 100%；Humulus grass fermentation liquid energy significantly improves Chinese leaf film clips
The content of green element prepares the romaine lettuce anthocyanidin content that humulus grass zymotic fluid dilutes the zymotic fluid after 4 times again using 100% embodiment 2
Romaine lettuce compared with the improvement Huo Gelan nutrient solutions for using 100% improves 1 times or more.
（3）Humulus grass fermentation liquid energy significantly increases the soluble sugar and Vc contents of romaine lettuce, wherein using 100% embodiment 2
It prepares humulus grass zymotic fluid and dilutes the romaine lettuce soluble sugar content of the zymotic fluid after 4 times again, using 75%（V/V）Embodiment 2 prepare
Humulus grass zymotic fluid dilutes the romaine lettuce Vc contents of the improvement Huo Gelan nutrient solutions of the zymotic fluid+25% after 4 times respectively than using 100% again
The romaine lettuce for improveing Huo Gelan nutrient solutions is high by 5.6%, 57.98%；Use 25%（V/V）Embodiment 2 prepare humulus grass zymotic fluid dilute again
The romaine lettuce of the improvement Huo Gelan nutrient solutions of zymotic fluid+75% after 4 times can significantly improve the soluble protein content of romaine lettuce, than making
Romaine lettuce yield with 100% improvement Huo Gelan nutrient fluid cultivations is high by 13.58%, and humulus grass zymotic fluid substitution ratio is at 50% or more
There is significantly reduced effect to romaine lettuce soluble protein content.
Influence experiment two of the humulus grass zymotic fluid to Growth of Lettuce and quality
1, test objective
This experiment makees foliar fertilizer using the humulus grass zymotic fluid of different extension rates and carries out romaine lettuce soilless culture, studies humulus grass zymotic fluid
Influence as foliar fertilizer to water planting Growth of Lettuce and its nutritional quality, to for humulus grass comprehensive exploitation and the organic leaf of plant source
The exploitation of leaven provides Technical Reference and theoretical foundation with application.
2, test material
Using humulus grass zymotic fluid after different multiples dilute prepared by the method in the embodiment of the present invention 2 carry out Growth of Lettuce and
The influence of quality is tested, and humulus grass fermentating liquid PH value 5.82, EC values 4.9, ingredient is as follows：Full nitrogen 294.38mg/L, full phosphorus
25.92mg/L, full potassium 174.18mg/L, soluble sugar 2283.1mg/L, soluble solid 0.8%.Romaine lettuce kind is that " meaning is big
Sharp romaine lettuce " is bred with Yi Feng standing grain seeds company；Using improvement Huo Gelan（Hogagland ＆ Apnon, 1938）Formula nutritional liquid
Formula.
3, test period, place
Field trial is 11 days~December 16 November in 2017 in the vinyl house of three area's two-membrane process of Agricultural University Of He'nan
It carries out；Lab index test experiments are 16 days~December 17 December in 2017 in College of Horticulture of Agricultural University Of He'nan laboratory
Interior progress.
4, test method
Using completely randomized experiment, five processing of A, B, C, D and CK are set altogether：
A：Humulus grass zymotic fluid prepared by embodiment 2 dilutes 12.5 times；
B：Humulus grass zymotic fluid prepared by embodiment 2 dilutes 25 times；
C：Humulus grass zymotic fluid prepared by embodiment 2 dilutes 50 times；
D：Humulus grass zymotic fluid prepared by embodiment 2 dilutes 75 times；
CK：Clear water.
By the way of hole plate seedling growth, seed is directly multicast in 50 hole hole trays, cave depth 1cm；It is divided into seedlings after 26 days, by seedling
Clear water is washed after root and is placed in customization cup with sponge block package, then in shady place slow seedling 2 days；By the seedling delayed, water is arrived in field planting at random
On training frame, start to process after field planting 7 days is primary at interval of processing in 7 days, and blade face whole of being subject to is moistened but do not dripped.It is entire raw
Chronic Intermittent is for nutrient solution, and daily 7:00-19:00 per hour for nutrient solution 15min, and 20:00-6:00 respectively at 24:00、6:
00 each feed flow 15min；Nocturnal temperature be less than 5 DEG C when with air heater wire below cultivating stand in 18:00-7:00 is heated.
12 plants are a processing, and each processing is repeated 3 times, the acquisition of 6 plants of progress related datas of every group of grab sample after field planting 35 days.
5, project and assay method are measured
Measure the item is as follows：Plant height（Basal part of stem is to romaine lettuce plant strain growth point）, base portion stem thickness（Basal part of stem to cotyledon interposition
It sets）, overground part dry weight and fresh weight of plant seedlings, the number of blade, maximum root long, root be living, soluble sugar in fresh leaf, soluble protein, Vc, nitrate, flower
Green cellulose content and chlorophyll（SPAD）With the relative amount of nitrogen in blade.
Measurement method is as follows：Plant height is surveyed with tape measure（Unit：cm）；Base portion stem thickness (unit is surveyed with electronic digital indicator：
mm)；With assay balance geodetic top dry weight and fresh weight of plant seedlings（Fresh weight is directly measured with balance, dry weight：105 DEG C of water-removing 15min, 85 DEG C of constant temperature
It weighs after drying to constant weight）；Number of blade number directly on plant（Leaf length disregarding less than 5cm）；It is surveyed with the ruler of accuracy 0.1cm
Maximum root long；TTC methods survey root and live；Anthrone method surveys soluble sugar content；Dying method with coomassie brilliant blue surveys soluble protein content；2,
6-D titrations survey Vc contents；Salicylic acid colorimetry surveys nitrate content；Absorbance method surveys anthocyanidin content；Chlorophyll element instrument is surveyed
Chlorophyll, Leaf N relative amount.
All test datas are all made of DPS7.05 softwares and carry out variance analysis, with 2016 softwares of WPS office into line number
According to statistics, it maps, and with LSD methods of inspection to significant difference (P<0.05) Multiple range test is carried out.
6, test result
The zymotic fluid of the different extension rates of table 1 makees influence tables of data of the foliar fertilizer to romaine lettuce overground part and maximum root long
As shown in Table 1, the plant height, stem thickness of romaine lettuce, maximum root long, ground fresh weight are all with the increase of humulus grass zymotic fluid extension rate elder generation
Increase after reduction, and be all spray D foliar fertilizers effect it is best, wherein spray D foliar fertilizers maximum root long be noticeably greater than compare,
The ground fresh weight for spraying the stem thickness of B, C foliar fertilizer and spraying B foliar fertilizers is substantially less than control；The number of blade respectively handled is not notable
Sex differernce, but the number of blade for spraying D foliar fertilizers is most；The overground part dry weight for spraying D foliar fertilizers and the difference of other processing reach aobvious
Level is write, and difference is not notable between other processing；Show：The humulus grass zymotic fluid of suitable concentration has promotion to make the growth of romaine lettuce
With the facilitation for spraying D foliar fertilizers is maximum, plant height, stem thickness, the number of blade, maximum root long, the relatively control point of overground part dry weight and fresh weight of plant seedlings
3.59%, 5.18%, 6.49%, 31.63%, 12.37%, 17.78% is not increased.
By Figure 13 and 14 it is found that with humulus grass zymotic fluid extension rate increase, romaine lettuce blade Determination of Chlorophyll, nitrogen are opposite to be contained
Amount is in incremental trend, but is below compareed, wherein spraying the chlorophyll of A, B, C foliar fertilizer and spraying the nitrogen phase of A, B foliar fertilizer
To content, difference is notable compared with the control, and spraying D foliar fertilizers, difference is not notable compared with the control, and relative amount compares respectively
Control reduces 4.16%, 0.97%；As shown in Figure 15, B, C, D foliar fertilizer is sprayed compared with the control to remarkably promote in romaine lettuce blade
The accumulation of anthocyanidin, wherein the effect for spraying D foliar fertilizers is best, content is higher by 78.57% than compareing；As shown in Figure 16, spray B,
The root activity of D foliar fertilizers is significantly higher than control, higher by 17.83%, 7.60% than compareing respectively, sprays A foliar fertilizer root activity
It is significantly substantially less than and compares；Show：The humulus grass zymotic fluid of high concentration inhibits the accumulation of romaine lettuce blade Determination of Chlorophyll, nitrogen, and humulus grass
Zymotic fluid dilution is bigger, and inhibiting effect is weaker；Humulus grass zymotic fluid extension rate had both promoted cyanine in romaine lettuce blade after being more than 50 times
The accumulation of element, and the root activity of romaine lettuce can be improved, the facilitation effect that D foliar fertilizers are sprayed for the accumulation of anthocyanidin is best, right
The effect that B foliar fertilizers are sprayed in the raising of root activity is best.
As shown in Figure 17, the soluble protein content respectively handled is above control, wherein spraying C, D foliar fertilizer and compareing
Difference reach the level of signifiance, it is higher by 27.90%, 42.47% than compareing respectively；As shown in Figure 18, A foliar fertilizers are sprayed and significantly inhibit life
The accumulation of nitrate in dish leaf piece sprays the accumulation that C foliar fertilizers remarkably promote nitrate in romaine lettuce blade, sprays B, D foliar fertilizer
It is not notable with the difference compareed；It appears from figure 19 that spraying the soluble sugar content of A, B, C, D foliar fertilizer, difference is equal compared with the control
Not significantly, wherein spraying the soluble sugar content of B, D foliar fertilizer respectively than having compareed high 17.05%, 13.64%, and B, D leaf are sprayed
Difference is not notable between leaven；As shown in Figure 20, the Vc contents for spraying A, B, C, D foliar fertilizer are above control, wherein spraying
B, D foliar fertilizers with the significant difference that compares, it is higher by 31.82%, 21.21% than compareing respectively, but spray it is poor between B, D foliar fertilizer
It is different not notable；Show：The humulus grass zymotic fluid of suitable concentration promotes the raising of lettuce quality, the raising for soluble protein content
The effect for spraying C, D foliar fertilizer is preferable, and the effect that B, D foliar fertilizer are sprayed for the raising of soluble sugar and Vc contents is preferable, right
The effect that A foliar fertilizers are sprayed in the reduction of nitrate content is best.
7, conclusion (of pressure testing)
This experiment makees foliar fertilizer using the humulus grass zymotic fluid of dilution different multiples, has studied it to water planting Growth of Lettuce and nutriment
The influence of matter, test result show：
1, the humulus grass zymotic fluid of suitable concentration has facilitation to the growth of romaine lettuce, the humulus grass zymotic fluid prepared using embodiment 2
The effect of 75 times of dilution is best, and plant height, stem thickness, the number of blade, maximum root long, the relatively control of overground part dry weight and fresh weight of plant seedlings increase respectively
3.59%、5.18%、6.49%、31.63%、12.37%、17.78%；
2, the humulus grass zymotic fluid of high concentration inhibits the accumulation of romaine lettuce blade Determination of Chlorophyll, nitrogen, is sent out using humulus grass prepared by embodiment 2
The inhibiting effect that zymotic fluid dilutes 75 times is most weak；
3, humulus grass zymotic fluid multiple had not only promoted the accumulation of anthocyanidin in romaine lettuce blade after being more than 12.5 times, but also can improve the root of romaine lettuce
It is vigor, the humulus grass zymotic fluid prepared using embodiment 2 dilutes 75 times of accumulation most useful for anthocyanidin, is prepared using embodiment 2
Humulus grass zymotic fluid dilute 25 times most useful for improve root activity, but using embodiment 2 prepare humulus grass zymotic fluid dilute 75 times
It is also remarkably improved root activity compared with control.
4. the humulus grass zymotic fluid of suitable concentration promotes the raising of lettuce quality, the humulus grass zymotic fluid prepared using embodiment 2
50 times of dilution, 75 times of raisings for being relatively conducive to soluble protein content, 25 times of the humulus grass zymotic fluid dilution prepared using embodiment 2,
75 times relatively be conducive to soluble sugar and Vc contents raisings, using embodiment 2 prepare humulus grass zymotic fluid dilute 12.5 times most useful for
Reduce nitrate content；
Analysis is to sum up stated it is found that humulus grass zymotic fluid prepared by embodiment 2 is in general used to make answering for foliar fertilizer after diluting 75 times
It is best with effect.In addition, humulus grass zymotic fluid makees foliar fertilizer has dual character, high concentration to the growth of romaine lettuce and its effect of quality
Humulus grass zymotic fluid inhibit romaine lettuce root activity and its blade Determination of Chlorophyll, nitrogen accumulation, but suitable concentration humulus grass fermentation
Liquid to promote romaine lettuce growth and its quality improvement effect it is notable, the reason of causing this phenomenon may be：One side and humulus
The accumulation of certain allelochemicals is related in careless zymotic fluid, on the other hand related with the growth regulatory substance in humulus grass zymotic fluid.This
Experiment makees influence of the foliar fertilizer to Growth of Lettuce and its quality after only having inquired into humulus grass source zymotic fluid dilution different multiples, in relation to it
The influence etc. of influencing mechanism and other effects need further to study.
Claims (9)
1. a kind of humulus grass zymotic fluid that can substitute mineral nutrition, which is characterized in that it is prepared by the raw material of following parts by weight
It forms：5~7 parts of humulus grass cauline leaf, 4~6 parts of brown sugar, 0.05~0.07 part of dregs of beans, 0.01~0.03 part of feldspar in powder, ground phosphate rock
0.01~0.03 part, 50~60 parts of water.
2. a kind of humulus grass zymotic fluid that can substitute mineral nutrition according to claim 1, which is characterized in that it be by with
The raw material of lower parts by weight is prepared：6 parts of humulus grass cauline leaf, 5 parts of brown sugar, 0.06 part of dregs of beans, 0.02 part of feldspar in powder, ground phosphate rock
0.02 part, 55 parts of water.
3. a kind of humulus grass zymotic fluid that can substitute mineral nutrition according to claim 1, it is characterised in that：The humulus grass
The length of cauline leaf is 2~3cm.
4. a kind of humulus grass zymotic fluid that can substitute mineral nutrition as described in claims 1 to 3 any one claim
Preparation method, it is characterised in that include the following steps：
（1）Humulus grass material guillotine cutting；
（2）It is prepared by brown sugar water；
（3）Fermented material mixes；
（4）Aerobic fermentation；
（5）Anaerobic fermentation；
（6）Dilution, filtering.
5. a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition as claimed in claim 4, it is characterised in that：
Step（2）In take a part of water in raw material, water temperature is increased to 25~30 DEG C, is put into brown sugar, brown sugar is completely dissolved obtained red
Syrup.
6. a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition as claimed in claim 4, it is characterised in that：
Step（3）It is middle to be first sufficiently mixed dregs of beans and humulus grass material, then it will add in the brown sugar water prepared, feldspar in powder and ground phosphate rock
Add remaining water in raw material, is stirred when pouring into.
7. a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition as claimed in claim 4, it is characterised in that：
Step（4）In every 3~5 days stir 1 time, fermentation the phase be 35 days.
8. a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition as claimed in claim 4, it is characterised in that：
Step（5）The middle fermentation phase is 15 days.
9. a kind of preparation method for the humulus grass zymotic fluid that can substitute mineral nutrition as claimed in claim 4, it is characterised in that：
Step（6）It is middle to be diluted using the water of 4 times of raw material gross weight, using >=40 the polished standard screens, natural filtration.
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