CN108310053A - The preparation method and application of Pogostemon cablin medicine materical crude slice - Google Patents

The preparation method and application of Pogostemon cablin medicine materical crude slice Download PDF

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Publication number
CN108310053A
CN108310053A CN201810435299.1A CN201810435299A CN108310053A CN 108310053 A CN108310053 A CN 108310053A CN 201810435299 A CN201810435299 A CN 201810435299A CN 108310053 A CN108310053 A CN 108310053A
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CN
China
Prior art keywords
pogostemon cablin
medicine materical
materical crude
crude slice
drying
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CN201810435299.1A
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Inventor
修彦凤
徐雯
王楠
汪颖舒
刘若楠
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Shanghai University of Traditional Chinese Medicine
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Shanghai University of Traditional Chinese Medicine
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Priority to CN201810435299.1A priority Critical patent/CN108310053A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/532—Agastache, e.g. giant hyssop
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
    • A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/13—Preparation or pretreatment of starting material involving cleaning, e.g. washing or peeling
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting

Abstract

The invention belongs to the field of Chinese medicines, and in particular to a kind of preparation method of Pogostemon cablin medicine materical crude slice takes clean fresh Pogostemon cablin medicinal material, daylight drying or in the ventilating drying equipment of 30 DEG C~40 DEG C temperature, continuous drying 5~7 hours；Afterwards under the covering of gas permeability covering, sweating 16~17 hours is stacked；The moisture on Pogostemon cablin medicinal material surface, cutting and drying, obtain Pogostemon cablin medicine materical crude slice after removal sweating.According to existing Habitat producing and concocting method, to optimize, obtained new method.This method prepare Pogostemon cablin medicine materical crude slice with《Chinese Pharmacopoeia》The quality of Pogostemon cablin medicine materical crude slice prepared by method is similar with Toxicity Analysis, and meets pharmacopoeial requirements, and the decocting liquid of Pogostemon cablin medicine materical crude slice prepared by the method for the present invention also has anti-inflammatory activity.

Description

The preparation method and application of Pogostemon cablin medicine materical crude slice
Technical field
The invention belongs to field of Chinese herbal medicine technology, and in particular to the preparation method and application of Pogostemon cablin medicine materical crude slice.
Background technology
Prepared slices of Chinese crude drugs processing directly affects its clinic diagnosis effect, and " processing is unknown, and pharmacological property is or not the record of China's classic of TCM Really, then soup side is without standard and illness is without testing." also demonstrate the viewpoint.It is aided with existing science and technology, improves the prepared slices of Chinese crude drugs and process Level improves drug concocted quality, ensures clinical drug treatment effect, reduces prepared slices of Chinese crude drugs processing cost, is modern Chinese herbal medicine neck The direction of domain effort for it.
Pogostemon cablin is the drying that Labiatae pierces stamen grass platymiscium Pogostemon cablin Pogostemon cablin (Blanco) Benth. Aerial part.Its acrid flavour, slightly warm in nature, returns spleen, stomach, lung channel have and eliminate dampness with aromatics and middle preventing or arresting vomiting, deliver relieving summer-heat effect.It cures mainly wet Turbid middle resistance, the vomiting of gastral cavity ruffian, summer-heat and damp exterior syndrome, damp-warm syndrome is from the beginning of burnout of generating heat is uncomfortable in chest not relax, and cold-dampness closes heat, and abdominal pain is vomited and diarrhoea, nasosinusitis head Pain etc..《Chinese Pharmacopoeia》The habitat processing method that version in 2015 records Pogostemon cablin is " to be tapped when branches and leaves are luxuriant, solarization night is bored, instead It is multiple extremely dry "；Concocting method is " to remove undesirable root and impurity, first tremble inferior lobe, well sifted is separately put；Stem is cleaned, and is moisturized, and segment is dried, then With leaf mixing ".
For Pogostemon cablin as traditional Chinese medicine of whole straw, crude drug is at source bored by solarization night, repeatedly to doing, obtains dry active compound Material.The Pogostemon cablin crude drug dried through Habitat producing, then by medicine materical crude slice enterprise water treating softener, cutting, drying, Chinese medicine can be become The prepared slices of Chinese crude drugs of clinical application.
From fresh medicinal material to medicine materical crude slice, Pogostemon cablin medicinal material is by two from fresh medicinal material to crude drug and from crude drug to medicine materical crude slice Secondary drying process.The solarization night bored equal drying process and water infiltration softening process being repeated, as armaticity Chinese medicine, effectively Easily with water loss, the active ingredients such as volatile oil can also scatter and disappear ingredient, and solarization night bored sweating effort can also make Pogostemon cablin medicinal material Internal component changes, and finally influences the quality of Pogostemon cablin medicinal material；And the storage and transport of crude drug are inconvenient.
Processing method in order to optimize Pogostemon cablin, the present invention integrate Habitat producing and process of preparing, reduce technique Step, Optimizing Process Parameters, to achieve the purpose that simplified technique, ensure prepared slice quality.
Invention content
The object of the present invention is to provide a kind of preparation methods of Pogostemon cablin medicine materical crude slice, and it is straight which takes fresh Pogostemon cablin It connects and prepares Pogostemon cablin medicine materical crude slice, integrate and optimize existing habitat processing method and Preparation process method, simplification is existing from fresh drug Drying and sweating process of the material to crude drug, remove water softening process of the existing Pogostemon cablin from crude drug to medicine materical crude slice, deduction and exemption are repeatedly In process, the loss of the active ingredients such as volatile oil in Pogostemon cablin ensures the quality of Pogostemon cablin medicine materical crude slice.
The technical scheme is that a kind of preparation method of Pogostemon cablin medicine materical crude slice, step include：Take the clean fresh wide leaves of pulse plants Incense drug material, daylight dry or in the ventilating drying equipments of 30 DEG C~40 DEG C temperature, continuous drying 5~7 hours；Afterwards ventilative Property covering covering under, stack sweating 16~17 hours；The moisture on Pogostemon cablin medicinal material surface, cutting and drying after removal sweating, Obtain Pogostemon cablin medicine materical crude slice.Specifically, after dry in daylight drying or ventilating drying equipment, the time interval for stacking sweating is carried out Be not easy it is too long, generally on the day of drying night or aeration-drying on the day of just carry out stacking sweating.
The cleaning method of fresh Pogostemon cablin medicinal material is：After fresh Pogostemon cablin medicinal material removal impurity and undesirable root, water cleaning is robbed simultaneously It drains.
During daylight drying, the temperature around Pogostemon cablin medicinal material is no more than 40 DEG C；It is preferred that at the 9~16 of illumination abundance The period is selected to be carried out continuously in period, atmospheric temperature is 20 DEG C~30 DEG C.
In stacking process, the layering of Pogostemon cablin medicinal material is staggeredly banked up, and the mutual not aliasing in leaf and root.
The gas permeability covering is woven cloth.Woven cloth good permeability, convenient for the gone out moisture of Pogostemon cablin medicinal material sweating Evaporation.
Sweating effort makes Pogostemon cablin medicinal material internal moisture escape, and is conducive to the drying of Pogostemon cablin medicinal material, while can also soften Pogostemon cablin medicinal material.
By daylight drying or ventilating drying equipment, Pogostemon cablin removing superficial moisture on medicinal materials after sweating is removed.In illumination abundance Daylight drying is carried out, the temperature around Pogostemon cablin medicinal material is no more than 40 DEG C, preferably 20 DEG C~35 DEG C；Dry 1~2 hour i.e. It can；Atmospheric temperature at this time is 20 DEG C~30 DEG C.The low temperature drying that 30 DEG C~40 DEG C are carried out in ventilating drying equipment, will not be right Pogostemon cablin ingredient causes to be lost in or convert to influence, and the dry time is 1~4 hour, preferably 1~2 hour.Preferred method is day Light dries, and solar radiation is sufficient, well-ventilated, while being conducive to moisture evaporation, proper temperature, avoid Pogostemon cablin fragrance ingredient or The volatilization of other compositions is lost in or conversion.
5~15mm sections are cut, two kinds of specifications of specially 5~10mm and 10~15mm.The short section of 5~10mm specifications is more Conducive to follow-up dry.
By daylight drying or ventilating drying equipment, Pogostemon cablin medicinal material after cutting is dried.In illumination abundance into Row daylight dries, and the temperature around Pogostemon cablin medicinal material is no more than 40 DEG C, preferably 20 DEG C~35 DEG C；The dry time is 2~6 small When, preferably 4~6 hours；Atmospheric temperature at this time is 20 DEG C~30 DEG C.The drying temperature of ventilating drying equipment is 45 DEG C~55 DEG C, preferably 50 DEG C；The dry time is 1.5~3 hours, preferably 2 hours；Low temperature drying processing, does not interfere with Pogostemon cablin Ingredient.
As a preferred embodiment of the present invention, the preparation method of Pogostemon cablin medicine materical crude slice includes：Fresh Pogostemon cablin medicinal material is taken, After removing impurity and undesirable root, robs water and clean and drain；1st day：At sun-drenched 9~16 in the period, continuous drying 6 is small When；Under woven cloth covering, night stacks sweating 16~17 hours；2nd day：Drying 1~2 hour；It is short to cut into 5~10mm Section, it is 2 hours dry in 50 DEG C of drying chambers, obtain Pogostemon cablin medicine materical crude slice.
The decocting liquid of Pogostemon cablin medicine materical crude slice prepared by the method for the present invention can reduce carrageenan and cause scorching rat paw edema Degree inhibits the abnormal of inflammatory mediator (NO, TNF-α, PGE2, MDA, IL-1 β) in rat blood serum to generate, has anti-inflammatory activity, and The anti-inflammatory activity of middle and high dosage group (6.48g crude drugs/kg, 12.96g crude drug/kg) is especially notable.
The decocting liquid of Pogostemon cablin medicine materical crude slice prepared by the method for the present invention can be used for preparing the drug with anti-inflammatory activity, as suppression The active site that inflammatory mediator (NO, TNF-α, PGE2, MDA, IL-1 β) processed generates, the exception for reducing inflammatory mediator in serum are raw At.
The present invention also provides a kind of pharmaceutical preparation with anti-inflammatory activity, the Pogostemon cablin medicine materical crude slice water for preparing in the process of the present invention Decocting liquid is as active constituent.
The pharmaceutical preparation further includes any one or more pharmaceutically acceptable auxiliary material, described pharmaceutically acceptable auxiliary Material has carrier, excipient, disintegrant, flavoring agent, lubricant, adhesive etc..
The pharmaceutical preparation further includes Supplementary active ingredients, and the Supplementary active ingredients are auxiliary prevention of inflammation active constituent Or auxiliary treatment inflammatory activity ingredient etc..
The pharmaceutical preparation, be prepared into as needed with prevent and/or therapeutic effect it is pharmaceutically acceptable arbitrary Dosage form.Pharmaceutically acceptable dosage form has liquid preparation, solid pharmaceutical preparation, semisolid preparation or gaseous formulation etc..Solid pharmaceutical preparation Including but not limited to pill, powder, sublimed preparation, granule, capsule, tablet, pulvis etc.；Liquid preparation includes but not limited to oral Liquid, injection, suspension, electuary etc.；Gaseous formulation includes but not limited to aerosol, spraying etc..Tablet includes but not limited to sugar Garment piece agent, film coated tablet, enteric coated tablet, lozenge etc.；Capsule includes but not limited to hard capsule and soft capsule.
Compared with the existing technology, the present invention has the following advantages：
The present invention provides a kind of preparation method of new Pogostemon cablin medicine materical crude slice, and this method process is easy, and technological parameter is clear, from Fresh Pogostemon cablin medicinal material directly prepares Pogostemon cablin medicine materical crude slice, compared to existing habitat processing method and Preparation process method twice work For sequence, reduce dry and sweating process, removes water removal softening process, avoid in dry, sweating and water softening process, volatile oil Etc. active ingredients be largely lost or change of fermenting；While optimizing existing Pogostemon cablin medicine materical crude slice preparation method, ensure Pogostemon cablin drink The quality of piece.
The decocting liquid of Pogostemon cablin medicine materical crude slice prepared by preparation method of the present invention, the rat foot that can effectively inhibit carrageenan to induce Plantar swelling, lower rat blood serum in NO, TNF-α, PGE2, MDA, IL-1 β level, to play anti-inflammatory activity.The wide leaves of pulse plants is provided The new pharmacological activity of fragrant medicine materical crude slice decocting liquid, the clinical treatment for Pogostemon cablin medicine materical crude slice decocting liquid provide new application.
Description of the drawings
Fig. 1 is the Pogostemon cablin in 3 Guangning, four meetings, spring places of production respectively according to embodiment 1 (the method for the present invention) and embodiment The photo of gained Pogostemon cablin medicine materical crude slice after the preparation method processing of 2 (official methods).A1 refers to the place of production prepared according to official method The Pogostemon cablin medicine materical crude slice of Guangning, B1 refer to according to the Pogostemon cablin medicine materical crude slice that the place of production prepared by official method is four meetings, and C1 refers to according to pharmacopeia side The place of production prepared by method is the Pogostemon cablin medicine materical crude slice in spring, and A2 refers to be drunk according to the Pogostemon cablin that the place of production prepared by the method for the present invention is Guangning Piece, B2 refer to according to the Pogostemon cablin medicine materical crude slice that the place of production prepared by the method for the present invention is four meetings, and C2 refers to the production prepared according to the method for the present invention Ground is the Pogostemon cablin medicine materical crude slice in spring.
Fig. 2 is the Pogostemon cablin in 3 Guangning, four meetings, spring places of production respectively according to embodiment 1 (the method for the present invention) and embodiment The preparation method of 2 (official methods) prepares gained Pogostemon cablin medicine materical crude slice and patchouli alcohol TLC figures；1 finger is prepared according to the method for the present invention The place of production be Guangning Pogostemon cablin medicine materical crude slice, 2 refer to the Pogostemon cablin medicine materical crude slice that the place of production prepared according to official method is Guangning, 3 fingers according to The place of production of the method for the present invention preparation is the Pogostemon cablin medicine materical crude slice of four meetings, and 4 refer to according to the wide leaves of pulse plants that the place of production prepared by official method is four meetings Fragrant medicine materical crude slice, 5 refer to according to the Pogostemon cablin medicine materical crude slice that the place of production prepared by the method for the present invention is spring, and 6 refer to the production prepared according to official method Ground is the Pogostemon cablin medicine materical crude slice in spring, and 7 refer to patchouli alcohol reference substance.
Fig. 3 is the HPLC spectrograms of n-hexane blank solvent.
Fig. 4 is the HPLC spectrograms of patchouli alcohol reference substance hexane solution.In figure, 1 is patchouli alcohol, and 2 be n-octadecane.
Fig. 5 is the hexane solution for the Pogostemon cablin medicine materical crude slice chloroform extract that the place of production prepared by the method for the present invention is Guangning HPLC spectrograms.In figure, 1 is patchouli alcohol, and 2 be n-octadecane.
Fig. 6 is the hexane solution for the Pogostemon cablin medicine materical crude slice chloroform extract that the place of production prepared by official method is Guangning HPLC spectrograms.In figure, 1 is patchouli alcohol, and 2 be n-octadecane.
Fig. 7 is the HPLC spectrograms of Pogostone methanol solution.In figure, A is Pogostone chromatographic peak.
Fig. 8 is the HPLC spectrograms for the Pogostemon cablin medicine materical crude slice methanol extract liquid that the place of production prepared by the method for the present invention is Guangning.In figure, A is Pogostone chromatographic peak.
Fig. 9 is the HPLC spectrograms for the Pogostemon cablin medicine materical crude slice methanol extract liquid that the place of production prepared by official method is Guangning.In figure, A For Pogostone chromatographic peak.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.These embodiments are interpreted as being merely to illustrate this hair It is bright rather than limit the scope of the invention.After having read the content of the invention recorded, those skilled in the art can To be made various changes or modifications to the present invention, these equivalence changes and modification are equally fallen into defined by claims of the present invention Range.
The present invention buy the Guangning in Guangzhou, four meeting, 3 different sources of spring Pogostemon cablin, according to Pogostemon cablin of the present invention drink Piece preparation method and 2015 editions《Chinese Pharmacopoeia》Habitat processing method and concocting method prepare Pogostemon cablin medicine materical crude slice respectively, and compare Analyze the character of medicine materical crude slice, thin layer discriminating, moisture, total ash, acid-insoluble ash, impurity, leaf, patchouli alcohol, Pogostone and The anti-inflammatory effect of alcohol extract and medicine materical crude slice decocting liquid.
1 the method for the present invention of embodiment prepares Pogostemon cablin medicine materical crude slice
The fresh Pogostemon cablin for taking 3 Guangning, four meetings, spring places of production, proceeds as follows respectively：Impurity and undesirable root are removed, The net silt of wash quickly, drains the water, and daylight dried 6 hours (atmospheric temperature was 20 DEG C~30 DEG C, when 9~15 or 10~16 daytime When), it stacks at night, with woven cloth covering sweating 16~17 hours, spreads within the 2nd day daylight out and dry 1~2 hour, with slice machine-cut 5 ~10mm short sections dry 2h to drying for 50 DEG C in drying chamber, dry in the air cool, Pogostemon cablin medicine materical crude slice is packed with polyethylene plastic bag.Guangning, four Meeting, 3 place of production Pogostemon cablin medicine materical crude slice of spring photo such as Fig. 1 in shown in A2, B2, C2.
Carry out on the day of this method a solarization night it is bored after, only need to remove removing superficial moisture on medicinal materials within the 2nd day, you can carry out cutting and It is dry, medicine materical crude slice is made.The method of removal removing superficial moisture on medicinal materials has dry in drying or ventilating drying equipment, it is generally preferable to traditional Drying.Ventilating drying equipment has air dry oven or ventilation drying chamber etc..
Drying refers in ventilation or shady place drying, generally daylight drying or shady and cool drying, and in a few days light fills for daylight drying The outdoor of foot spreads solarization out, and cool place drying refers to draughty shady and cool building, shady and cool structures or natural shady place and spreads solarization out. It is dried relative to cool place, the effect of daylight drying is the preferred of traditional method for airing more.Since Pogostemon cablin has armaticity, During daylight dries, Pogostemon cablin ambient temperature ensures to be no more than 40 DEG C.
In stacking process, the layering of Pogostemon cablin medicinal material is staggeredly banked up, and the mutual not aliasing in leaf and root.
2 official method of embodiment prepares Pogostemon cablin medicine materical crude slice
Take the fresh Pogostemon cablin in 3 Guangning, four meetings, spring places of production, respectively according to《Chinese Pharmacopoeia》The Habitat producing of record Method is prepared with concocting method：Solarization night is bored, repeatedly to dry；Undesirable root and impurity are removed, first trembles inferior lobe, well sifted is separately put；Stem Clean, moisturize, segment is dried, then with leaf mixing, Pogostemon cablin medicine materical crude slice is packed with polyethylene plastic bag.Guangning, four meetings, spring 3 In the photo such as Fig. 1 of a place of production Pogostemon cablin medicine materical crude slice shown in A1, B1, C1.
3 the method for the present invention of embodiment prepares the comparative studies of Pogostemon cablin medicine materical crude slice with official method
1. character
Pogostemon cablin medicine materical crude slice prepared by the method for the present invention is in irregular short section.Stem is slightly in square column type, and the most of ash in surface is brown Color, a small amount of lark, by pubescence.There is white marrow in section.Leaf is broken or shrinkage is agglomerating, celadon, in oval after complete person's flattening Or ellipse, two sides is by canescence villus；Base portion wedge shape or blunt circle, edge have the irregular cognate of size；Petiole is thin, soft Hair.Gas is fragrant special, mildly bitter flavor.
Pogostemon cablin medicine materical crude slice prepared by official method is in irregular section.Stem is slightly in square column type, surface lark or with reddish brown Color, by pubescence.There is white marrow in section.Leaf is broken or shrinkage is agglomerating, taupe or celadon, in oval or ellipse after complete person's flattening Circle, two sides is by canescence villus；Base portion wedge shape or blunt circle, edge have the irregular cognate of size；Petiole is thin, by pubescence. Gas is fragrant special, mildly bitter flavor.
2. thin layer differentiates
Example 1 and the Pogostemon cablin medicine materical crude slice coarse powder of embodiment 2 are appropriate, respectively according to determination of volatile oil method (《Chinese Pharmacopoeia》 General rule 2204) it measures, divide and take volatile oil 0.5ml, adds ethyl acetate to be diluted to 5ml, as test solution.Separately take patchouli alcohol Reference substance adds ethyl acetate that solution of every 1ml containing 2mg is made, as a contrast product solution.According to thin-layered chromatography (《Chinese Pharmacopoeia》 General rule 0502) experiment, above-mentioned solution respectively draws 1~2 μ l, put respectively on same silica gel g thin-layer plate, with petroleum ether (30~60 DEG C)-ethyl acetate-glacial acetic acid (95：5：0.2) it is solvent, is unfolded, take out, dry, sprays with 5% ferric trichloride ethanol solution. A yellow spotting (volatile oil put long has, brand-new without) is shown in test sample chromatography；It is clear to be heated to spot development, test sample color In spectrum, on position corresponding with reference substance chromatography, identical hyacinthine spot is shown.New method is processed to be prepared extensively with official method The shade of wrinkled giant hyssop medicine materical crude slice spot does not have notable difference, as shown in Figure 2.
3. checking
According to《Chinese Pharmacopoeia》Version method measures within 2015,《Chinese Pharmacopoeia》It provides that Pogostemon cablin is impure, 2% must not be crossed； Moisture must not cross 14.0%；Total ash must not cross 11.0%, and acid-insoluble ash must not cross 4.0%；Leaf must not be less than 20%. The inspection item for the Pogostemon cablin medicine materical crude slice that Pogostemon cablin medicine materical crude slice prepared by the method for the present invention is prepared with official method meets the requirements.
1 the method for the present invention of table prepares the inspection measurement result of Pogostemon cablin medicine materical crude slice with official method
4. the measurement result of patchouli alcohol, Pogostone, alcohol extract
The measurement of patchouli alcohol content and alcohol extract, according to《Chinese Pharmacopoeia》Version method is measured within 2015, hundred autumns Lee's alcohol content must not be less than 0.1%, and alcohol extract content must not be less than 2.5%.The chromatographic condition that Pogostone measures： Diamonsil C18 chromatographic columns (250 × 4.6mm, 5 μm), mobile phase are acetonitrile (A)：0.1% acetic acid-water (B), gradient elution (table 2), flow velocity 1.0ml/min, column temperature are 30 DEG C, Detection wavelength 310nm, and sample size is 10 μ L.
The place of production prepared by n-hexane blank solvent, patchouli alcohol reference substance hexane solution, the method for the present invention is Guangning The hexane solution of Pogostemon cablin medicine materical crude slice chloroform extract and the Pogostemon cablin medicine materical crude slice three that the place of production prepared by official method is Guangning The HPLC spectrograms of the hexane solution of chloromethanes extract are successively as shown in Fig. 3, Fig. 4, Fig. 5 and Fig. 6, and in figure, 1 is hundred autumn Lee Alcohol, appearance time 15.01min, 2 be n-octadecane, appearance time 24.97min.
The place of production prepared by Pogostone reference substance methanol solution, the method for the present invention is that the Pogostemon cablin medicine materical crude slice methanol of Guangning extracts The place of production of liquid and official method preparation is the HPLC spectrograms of the Pogostemon cablin medicine materical crude slice methanol extract liquid of Guangning successively such as Fig. 7, Fig. 8 and figure Shown in 9, the appearance time of Pogostone (A) is 58.80min.
By the comparison of Fig. 5 and Fig. 6, Fig. 8 and Fig. 9 it is found that relative to Pogostemon cablin medicine materical crude slice prepared by official method, side of the present invention Other constituents and content of Pogostemon cablin medicine materical crude slice prepared by method, there is significantly different and differences.
2 gradient elution program table of table
3 the method for the present invention of table prepares the survey of patchouli alcohol, Pogostone, alcohol extract in Pogostemon cablin medicine materical crude slice with official method Determine result
The method of the present invention prepare Pogostemon cablin medicine materical crude slice and official method prepare Pogostemon cablin medicine materical crude slice patchouli alcohol content and Alcohol extract content meets pharmacopoeial requirements, and the method for the present invention prepares the content of the alcohol extract of Pogostemon cablin medicine materical crude slice and is apparently higher than Pogostemon cablin medicine materical crude slice prepared by official method.
5. brief summary
The main table of quality difference for the Pogostemon cablin medicine materical crude slice that Pogostemon cablin medicine materical crude slice prepared by the method for the present invention is prepared with official method The difference of present appearance color, the content of other indexs such as impurity, ash content, moisture, patchouli alcohol and Pogostone be not apparent Difference illustrates that the Pogostemon cablin medicine materical crude slice of the method for the present invention preparation reaches the quality requirement for the Pogostemon cablin medicine materical crude slice that Chinese Pharmacopoeia records.
4 the method for the present invention of embodiment prepares the pharmacological activity comparative study of Pogostemon cablin medicine materical crude slice with official method
1. the method for the present invention prepares the decocting liquid of Pogostemon cablin medicine materical crude slice
The fresh Pogostemon cablin for taking Guangning prepares Pogostemon cablin medicine materical crude slice according to the method that embodiment 1 is recorded；Pogostemon cablin medicine materical crude slice is taken, 10 times of amount water are added and impregnate 0.5h, using double formulations, decoction is not further added by volatile oil, collects volatile oil, is separately done him and is used；Filter It crosses, obtains decocting liquid and filter residue；Filter residue adds 8 times of water to decoct 0.5h again, and filtration obtains decocting liquid；Merge secondary decocting liquid, concentrates, obtain 1.296g crude drugs/ml extracting solutions are added isometric distilled water and dilute successively, obtain 0.324g crude drugs/ml extracting solutions (low concentration), 0.648g crude drugs/ml extracting solutions (middle concentration), 1.296g crude drugs/ml extracting solutions (high concentration), it is spare.2. official method prepares wide The decocting liquid of wrinkled giant hyssop medicine materical crude slice
The fresh Pogostemon cablin for taking Guangning, according to version in 2015《Chinese Pharmacopoeia》The habitat processing method and concocting method of record Prepare Pogostemon cablin medicine materical crude slice；Pogostemon cablin medicine materical crude slice is taken, 10 times of amount water are added and impregnate 0.5h, using double formulations, decoct to volatile oil no longer Increase, collect volatile oil, separately does him and use；Filtration, obtains decocting liquid and filter residue；Filter residue adds 8 times of water to decoct 0.5h again, and filtration obtains water Decocting liquid；Merge secondary decocting liquid, concentrates, obtain 1.296g crude drugs/ml extracting solutions, isometric distilled water is added and dilutes successively, obtains 0.324g crude drugs/ml extracting solutions (low concentration), 0.648g crude drugs/ml extracting solutions (middle concentration), 1.296g crude drugs/ml extracting solutions (high concentration), it is spare.
3. the anti-inflammatory effect of Pogostemon cablin medicine materical crude slice decocting liquid is studied
(1) modeling method
After rat adaptable fed 3d, it is randomly divided into Normal group, model control group, positive control medicine group, low Dosage group, middle dose group, high dose group, every group 8.Every group of rat administration rear right metapedes is measured using toes capacity measurer to cause Ankle-joint volume below before inflammation.Positive controls press aspirin 200mg/kg gastric infusions, basic, normal, high dose of Pogostemon cablin group Amount respectively press 3.24,6.48,12.96g crude drugs/kg gastric infusions, 1 time/d, continuous 7d, wherein Normal group and model comparison Group is to isometric distilled water.It is marked in the hot-tempered periarticular of hind leg with marking pen before last dose, after last dose 30min, It is water-soluble under vola pedis key film to inject 1% carrageenan physiology salt behind the right side of high, medium and low dosage group animal for model group, positive controls Liquid (0.1ml), blank group do not give cause scorching processing, are put to death after 6h.Period measured using toes capacity measurer cause it is scorching after 1h, The sufficient volume of 2h, 3h, 4h, 5h and 6h carry out foot swelling degree measurement.Calculate foot swelling percentage and foot swelling inhibiting rate.
Foot plantar volume × 100% before foot swelling percentage=(foot plantar volume before foot plantar volume-injection after injection)/injection
Foot swelling inhibiting rate=(model group be averaged paw swelling-administration group be averaged paw swelling)/model group average foot is swollen Expansibility × 100%
(2) measurement of inflammatory mediator
Abdominal aortic blood after being anaesthetized with 25% Ethylurethanm after the scorching rat 6h of cause stands 1h, 4000r/min centrifugations at room temperature 10min takes -80 DEG C of refrigerators of serum to preserve, spare.Interleukin I L-1 β (IL-1 β), tumor necrosis factor-alpha (TNF- are measured respectively α), prostaglandin E2 (PGE2), the concentration of nitric oxide (NO), malonaldehyde (MDA).
(3) experimental result
4, table 5 and table 6 must be the results are shown in Table by carrying out variance analysis using SPSS 17.0.
4 Pogostemon cablin of table to rat paw edema degree influence (N=8)
Note：Compared with model group,*P ＜ 0.05,**P ＜ 0.01
5 Pogostemon cablin of table to rat paw inhibiting rate influence (N=8)
6 Pogostemon cablin of table to rat inflammation medium influence (N=8)
Note：Compared with normal group,ΔP ＜ 0.05,ΔΔP ＜ 0.01；Compared with model group,*P ＜ 0.05,**P ＜ 0.01
(4) brief summary
By table 4 and table 5 it is found that the method for the present invention prepares Pogostemon cablin medicine materical crude slice decocting liquid prepares Pogostemon cablin medicine materical crude slice with official method The rat toes swelling inhibitory activity of decocting liquid is similar, is：Compared with model group, various dose (basic, normal, high) Pogostemon cablin is given Medicine group can reduce carrageenan and scorching rat paw edema degree, middle dose group and high dose group is caused to reduce carrageenan and cause inflammation big The effect of mouse swelling degree of the paw is especially notable, and close to the rat toes swelling inhibition of positive controls.
As shown in Table 6, the method for the present invention prepares Pogostemon cablin medicine materical crude slice decocting liquid and prepares Pogostemon cablin medicine materical crude slice decocting with official method Liquid is similar to the inhibitory activity of rat inflammation medium, is：Compared with model group, various dose (basic, normal, high) Pogostemon cablin administration group Carrageenan can be reduced and cause inflammatory mediator (NO, TNF-α, PGE2, MDA, IL-1 β) concentration in scorching rat blood serum, middle dose group It is especially notable with the reduction of high dose group effect, and close in normal rats serum each inflammatory mediator concentration.
To sum up, the method for the present invention prepares the decocting liquid that the decocting liquid of Pogostemon cablin medicine materical crude slice prepares Pogostemon cablin medicine materical crude slice with official method Carrageenan can be reduced and cause scorching rat paw edema degree, and can inhibit carrageenan and cause inflammatory mediator in scorching rat blood serum The abnormal of (NO, TNF-α, PGE2, MDA, IL-1 β) generates, and has anti-inflammatory activity.Have relative to only Herba Pogostemonis Volatile oil anti-inflammatory Active existing research, present invention is disclosed the decocting liquids of Pogostemon cablin medicine materical crude slice equally to have anti-inflammatory activity.For the clinic of Pogostemon cablin Treatment and medicament exploitation provide new application.

Claims (10)

1. a kind of preparation method of Pogostemon cablin medicine materical crude slice, which is characterized in that step includes：Take clean fresh Pogostemon cablin medicinal material, day Light dries or in the ventilating drying equipment of 30 DEG C~40 DEG C temperature, continuous drying 5~7 hours；Afterwards in gas permeability covering Under covering, sweating 16~17 hours is stacked；The moisture on Pogostemon cablin medicinal material surface, cutting and drying, obtain the wide leaves of pulse plants after removal sweating Fragrant medicine materical crude slice.
2. preparation method according to claim 1, which is characterized in that the cleaning method of fresh Pogostemon cablin medicinal material is：It is fresh After Pogostemon cablin medicinal material removes impurity and undesirable root, robs water and clean and drain.
3. preparation method according to claim 1, which is characterized in that during daylight drying, around Pogostemon cablin medicinal material Temperature is no more than 40 DEG C.
4. preparation method according to claim 1, which is characterized in that pass through daylight drying or ventilating drying equipment, removal Pogostemon cablin removing superficial moisture on medicinal materials after sweating；During daylight drying, temperature around Pogostemon cablin medicinal material is no more than 40 DEG C, and the time is 1~2 hour；The drying temperature of ventilating drying equipment is 30 DEG C~40 DEG C, and the time is 1~4 hour.
5. preparation method according to claim 1, which is characterized in that cut 5~15mm sections.
6. preparation method according to claim 1, which is characterized in that by daylight drying or ventilating drying equipment, to cutting Pogostemon cablin medicinal material is dried after system；During daylight drying, the temperature around Pogostemon cablin medicinal material is no more than 40 DEG C, the time 2 ~6 hours；The drying temperature of ventilating drying equipment is 45 DEG C~55 DEG C, and the time is 1.5~3 hours.
7. the decocting liquid of the Pogostemon cablin medicine materical crude slice prepared by any one of claim 1-6 methods is preparing anti-inflammatory activity drug side The application in face, which is characterized in that the Pogostemon cablin medicine materical crude slice decocting liquid is as the active constituent for inhibiting inflammatory mediator to generate.
8. application according to claim 7, which is characterized in that the inhibition inflammatory mediator is NO, TNF-α, PGE2, MDA Or IL-1 β.
9. a kind of pharmaceutical preparation with anti-inflammatory activity, which is characterized in that the wide leaves of pulse plants prepared in any one of claim 1-6 methods The decocting liquid of fragrant medicine materical crude slice is as active constituent.
10. pharmaceutical preparation according to claim 9, which is characterized in that further including any one or more can pharmaceutically connect The auxiliary material and/or Supplementary active ingredients received.
CN201810435299.1A 2018-05-09 2018-05-09 The preparation method and application of Pogostemon cablin medicine materical crude slice Pending CN108310053A (en)

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