CN108271610B

CN108271610B - Method for planting Phoebe Machilus and application of extract thereof

Info

Publication number: CN108271610B
Application number: CN201810171884.5A
Authority: CN
Inventors: 开福根
Original assignee: Kai Fugen
Current assignee: Kai Fugen
Priority date: 2018-03-01
Filing date: 2018-03-01
Publication date: 2022-03-04
Anticipated expiration: 2038-03-01
Also published as: CN108271610A

Abstract

The invention relates to the field of new technologies, in particular to a method for planting a photinia fraseri and application of an extract of the photinia fraseri. The planting method at least comprises the following steps: (1) selecting soil with a pH value of 7-9 in a semi-negative and semi-positive land with a gradient of 15-30 degrees; (2) selecting seeds without diseases and insect pests, sterilizing the seeds with 8wt% of hydrogen peroxide solution for 15min, taking out and drying in the sun; (3) planting the seeds sterilized in the step (2) and safflower in a spacing way on the land selected in the step (1); (4) applying a fertilizer to the phoebe zhennan; (5) keeping the average temperature of the environment at 14-16 ℃.

Description

Method for planting Phoebe Machilus and application of extract thereof

Technical Field

The invention relates to the field of new technologies, in particular to a method for planting a photinia fraseri and application of an extract of the photinia fraseri.

Background

The Machilus thunbergii, also called as scaly wood, sunflower seeds and syzygium jambos, is a shrub or small arbor plant of syzygium genus of Myrtaceae family, and is originally produced in provinces such as Anhui, Zhejiang, Jiangxi and Guizhou provinces. The Machilus thunbergii has beautiful tree appearance, old stumps are cang Gu powerful, and the ornamental value of gardens is high. The Machilus thunbergii blooms in 6-8 months, most common, and a large number of white cymbidium floribundum flowers are blossomed, and flowers are like a scene in the full-bloom stage; the fruit turns to reddish red from light green in 8-11 months, the fruit is purple black when ripe, and the egg-shaped berries are full of branches and heads just like peach full trees. At present, the photinia fraseri is used for manufacturing the potted landscape by hedgerow, and the wonderful ancient potted landscape has high artistic value. In addition, the wood of the phoebe nanmu is fine and hard in texture and excellent in toughness, and can be used for manufacturing handles of common appliances or artworks and the like. At present, 80% of bonsais in the flower market of Jiangxi province are of the species of the Machilus thunbergii, and people can see the love of the Machilus thunbergii bonsais. After long-time cultivation, a complete industrial chain for cultivating, processing and modeling the machilus thunbergii has been formed in some areas of Jiangxi province, and taking the West Bay Xinxiancun of Nanchang city as an example, the pile of the machilus thunbergii is planted in the village to reach tens of thousands of plants in 2008. However, at present, most of main concerns about the phoebe-nanmu are only about how to model and make landscapes and how to cultivate more ornamental phoebe-nanmu bonsais, and researches on disease treatment, medicine value and the like of the phoebe-nanmu are rarely reported. Even if the effects of the phoebe-kanehirae thunb on other aspects are concerned, the phoebe-kanehirae thunb stays on leaves, rhizomes and the like of the phoebe-kanehirae thunb more frequently, and the research on the fruit of the phoebe-kanehirae thunb is less. Moreover, as most people pay attention to the beauty and the shape of the phoebe-zhennan, more attention is paid to the growth of stems and leaves in the planting process, and research on the conditions of influencing factors such as when the phoebe-zhennan blooms, how long the flower-growing period is, the high and low fruit setting rate, the influencing factors of fruit nutrient content and the like is neglected.

The inventor considers the actual research from 2008 to Fujian, Jiangxi, Guizhou, Anhui and the like, knows various types, habits and growth environments of the phoebe niphyllum, knows the effects of appreciation and the like of the prior phoebe niphyllum, realizes that the phoebe niphyllum has been ignored for a long time, and solves the problems of other applications, specific culture and the like of the phoebe niphyllum. Then, after years of continuous effort, repeated groping, practice and study, the soil quality and the terrain (such as slope, altitude and the like) which are most suitable for cultivating the machilus thunbergii are researched, for example, the research in Chinese patent CN201110276518.4, namely a ligusticum seedling culture method, finds that the acid soil on the land of semiyin and semiyang is beneficial to the growth of the ligusticum sinense; temperature required for planting and culturing; natural fertilizer required by the growth of the phoebe zhennan; how to plant the trees and other crops in a sleeving way to improve the fruit setting rate and the yield; how to enable the finally obtained phoebe nanmu fruits to have more medical value and the like, and finally researches a culture technology and a method for culturing phoebe nanmu with high medical application value.

Disclosure of Invention

In view of the above technical problems, a first aspect of the present invention provides a method for planting a machilus thunbergii, the method at least includes the following steps:

(1) selecting soil with a pH value of 7-9 in a semi-negative and semi-positive land with a gradient of 15-30 degrees;

(2) selecting seeds without diseases and insect pests, sterilizing the seeds with 8wt% of hydrogen peroxide solution for 15min, taking out and drying in the sun;

(3) planting the seeds sterilized in the step (2) and safflower in a spacing way on the land selected in the step (1);

(4) applying a fertilizer to the phoebe zhennan;

(5) keeping the average temperature of the environment at 14-16 ℃.

As a preferable technical scheme, the natural fertilizer comprises the following components in parts by weight: 30-40 parts of plant ash, 10-15 parts of rice hull, 10-20 parts of peanut shell, 25-40 parts of grass carbon, 5-10 parts of kaolin, 5-10 parts of cassava powder, 40-60 parts of straw and 5-8 parts of seaweed extract fertilizer.

As a preferable technical scheme, the percentage of the trees of the phoebe nankingense is 80-85% based on the total trees of the phoebe nankingense and the safflower.

As a preferred technical scheme, the soil is yellow core soil.

As a preferable technical scheme, the fertilizing time of the natural fertilizer is 20 days before the flowering period and 100-110 days after the flowering.

As an optimal technical scheme, the dosage of the natural fertilizer is 600-800 kg/mu.

The second aspect of the invention provides a phoebe nana extract obtained by the planting method, and the extraction method of the phoebe nana extract comprises the following steps:

(1) taking dried leaves of Machilus thunbergii 3g and Machilus thunbergii 10g, pulverizing, and mixing;

(2) adding 50ml of petroleum ether into the powder, refluxing for 45min, filtering, and discarding the filtrate to obtain filter residue;

(3) adding 90ml of distilled water into the filter residue obtained in the step (2), heating and refluxing, extracting for 3 hours, and cooling;

(4) and (3) adding 120ml of 95wt% ethanol and 1-3 g of an auxiliary agent into the system in the step (3), extracting for 1 hour under the microwave power of 250W, standing for 10min, filtering, concentrating the filtrate, and drying to obtain the machilus thunbergii extract.

As a preferable technical scheme, the auxiliary agent is tetrahydropiperine.

The third aspect of the invention provides application of the phoebe nana obtained by the planting method, and the phoebe nana is applied to scald, diabetes, bronchiectasis, prostatitis and asthma.

The fourth aspect of the invention provides application of the machilus thunbergii extract in treating bronchiectasis and diabetes.

The above-described and other features, aspects, and advantages of the present application will become more apparent with reference to the following detailed description.

Has the advantages that: the existing planting method of the phoebe-leaved sweetgum mainly focuses on regulating and controlling the appearance and the modeling landscaping of the phoebe-leaved sweetgum. Compared with the prior art, the planting method is simple and easy to operate. In addition, natural fertilizer is used in the planting process, other organic fertilizers are not added, and chemical components harmful to the soil, such as pesticides and herbicides, are not required to be sprayed, so that the quality of the soil can be protected, and the components in the soil and the surrounding environment are not damaged. Even if organic fertilizers are not used in the planting process, the effects of high plant survival rate, quick growth, no pests on leaves, high fruit setting rate, high fruit yield and the like can be achieved.

In addition, the effective components in the fruits and leaves of the phoebe nanmu planted according to the method provided by the invention are high in content, and the phoebe nanmu is rich in components which are beneficial to treating diseases such as bronchiectasis and diabetes. When the extract obtained by the extraction method provided by the invention is used, the proliferation and differentiation of pathogenic bacteria such as aspergillus, streptococcus pneumoniae, staphylococcus aureus and pseudomonas aeruginosa which cause scald infection, bronchiectasis combined infection and other diseases can be inhibited. And the obtained extract is taken by several diabetics for 8 weeks, so that the fasting blood sugar and the 2-hour postprandial glycemic index of the diabetics are greatly reduced, and the glycemic index of the diabetics can be stabilized and controlled to a certain extent, and the condition of the diabetics can be controlled.

Detailed Description

The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.

The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in a claim, the phrase is intended to claim as closed, meaning that it does not contain materials other than those described, except for the conventional impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.

When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.

The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "any" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.

Approximating language, as used herein throughout the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes portions that are literally received for modification without substantial change in the basic function to which the invention is related. Accordingly, the use of "about" to modify a numerical value means that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.

In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.

In order to solve the above technical problems, a first aspect of the present invention provides a method for planting a machilus thunbergii, the method at least comprising the following steps:

(4) applying a natural fertilizer to the phoebe zhennan;

(5) keeping the average temperature of the environment at 14-16 ℃.

The Machilus thunbergii, also called as scaly wood, sunflower seeds and syzygium jambos, is a shrub or small tree plant of Syzygium genus of Myrtaceae family. The variety of the machilus thunbergii is various, for example, the machilus thunbergii with two opposite leaves, the machilus thunbergii with three-night rotation, the machilus thunbergii with small leaves, the machilus brevifolia with similar bark and flower and fruit, and the like, mainly characterized in that the leaves are different, and the machilus thunbergii with two opposite leaves is planted by the applicant. The applicant selects different slopes and different daylights such as flat lands and hills and soils with different pH values such as yellow subsoil, black sandy soil and red soil to plant the phoebe-rich trees in areas such as Anji county and Deqing county of Huzhou. The acidity and alkalinity of the soil can be adjusted by spreading ferrous sulfate powder or lime and lime wall powder and other substances before planting the phoebe-nanmu seeds, and then plowing and loosening the soil. Through years of planting actual combat, applicants find that the alkalescent yellow-core soil is beneficial to the growth and fruit setting rate of the phoebe-kanehensis tree and improves the fruit yield of the phoebe-kanehensis tree. Although the phoebe nana plants grow earlier and faster in neutral or acid soil, especially acid soil, the phoebe nana plants bloom later and have short flowering period, low fruit setting rate and low final yield. Moreover, the applicant has unexpectedly found that the action of the phoebe nanmu fruit and leaves planted in the acid soil on bronchiectasis, diabetes and the like is not obvious, and the inhibition rate of pathogenic bacteria causing the diseases, such as aspergillus, streptococcus pneumoniae, staphylococcus aureus and pseudomonas aeruginosa, is low. The possible reason is that the components such as alkali metal oxides, trace elements, rare earth elements and the like contained in the alkaline yellow-heart soil change the way of the photinia fraseri roots to absorb nutrients or are beneficial to selectively absorbing certain special nutrients, so that the final fruit contains certain special components, and is beneficial to reducing the blood glucose index and inhibiting the proliferation and differentiation of certain germs.

The main component of the yellow sand soil is silicon dioxide, which is evolved from silica, wherein the nutrient components which are beneficial to plant growth are less harmful to the plant growth. Black soil is formed by strong humus accumulation and stagnant water retention, and is a special meadow chemical process. Laterite contains more iron and aluminum oxides, but the laterite is lack of alkali metals and alkaline earth metals, has strong leaching effect and less nutrients in minerals, and is easy to generate harmful metals such as aluminum, manganese and the like. Heavy land, more sand grains and poor fertilizer retention performance. The loess mineral component includes clastic mineral, clay mineral, authigenic mineral, etc. The clastic minerals are mainly quartz, feldspar and mica, and account for 80% of the clastic minerals. Secondly, pyroxene, amphibole, green curtain stone, chlorite, magnetite, etc.; in addition, loess contains a large amount of carbonate minerals, mainly calcite. The clay minerals mainly comprise illite, montmorillonite, kaolinite, goethite, hydrous hematite and the like. The chemical components of loess are also silicon dioxide, and the next is aluminum oxide, calcium oxide, ferric oxide, magnesium oxide, potassium oxide, ferric oxide, titanium dioxide, manganese oxide, etc.

The planting method of the invention can achieve the effect of semi-yin and semi-yang land by selecting the land with certain gradient such as hilly land, and the like, and can achieve the same effect by building a shed to shade sunlight if the light is too strong or the sunshine time is too long. The difference of the environmental temperature can also affect the growth of the phoebe nanmu plants and the components and contents in the fruits obtained after the phoebe nanmu plants grow to a certain extent. The applicant finds that the fruit yield, the survival rate and the like of the photinia serrulata are high when the annual average temperature of the environment is 14-16 ℃ after the photinia serrulata is planted in different environments such as Jiangxi, Jilin and the like at different temperatures. And the Huzhou region has mild temperature and relatively small annual average temperature fluctuation at 13-18 ℃, and is beneficial to planting high-quality phoebe-nanmu trees according to the method provided by the application.

In a preferred embodiment, the natural fertilizer comprises, in parts by weight: 30-40 parts of plant ash, 10-15 parts of rice hull, 10-20 parts of peanut shell, 25-40 parts of grass carbon, 5-10 parts of kaolin, 5-10 parts of cassava powder, 40-60 parts of straw and 5-8 parts of seaweed extract fertilizer.

In a preferred embodiment, the percentage of the plants of the machilus thunbergii is 80% -85% based on the total plants of the machilus thunbergii and the safflower.

In a preferred embodiment, the soil is selected from the group consisting of yellow core soil.

In a preferred embodiment, the natural fertilizer is applied 20 days before the flowering period and 100-110 days after the flowering period.

In a preferred embodiment, the amount of the natural fertilizer is 600 kg/mu to 800 kg/mu.

The inventor finds that compared with common organic fertilizers such as urea and the like, the natural fertilizer adopted in the process of planting the phoebe nanmu is more beneficial to the absorption of comprehensive nutrition by phoebe nanmu plants, absorbed nutrient substances are more beneficial to the absorption of fruits, the yield is improved, meanwhile, some effective components of the fruits are changed, and the medical value of the fruits is improved. The flowering of the photinia fraseri is about one month earlier than that of the photinia fraseri, and the pollination speed of the photinia fraseri can be increased when the photinia fraseri and the photinia fraseri are planted in a sleeving manner, so that the fruit setting rate is improved, and the yield is increased. The method is characterized in that the Phoebe dorsalis continues to come out of the soil after about 35-55 days after the Phoebe dorsalis seeds are planted, then the Phoebe dorsalis grows rapidly and blooms, the flowering period is about 105 days, natural fertilizers are applied about 20 days before the flowering period and at the end of the flowering period, the full flowering of the Phoebe dorsalis is facilitated, pollinated flowers are improved to grow into fruits, and if the fertilizers are applied in the flowering period, the plants grow excessively, branches and leaves are luxuriant, the flowers bloom is less, and the fruit yield is influenced.

The plant ash in the invention is the residue left by burning the plants, and can be obtained by removing weeds in the heaven and earth, collecting and burning. The rice hulls are byproducts in the rice processing process, are rich in components such as protein, sugar and the like, and can be purchased from the market; the peanut shell is the outer layer of the peanut, and can be used after being purchased and crushed or directly used. Peat, also known as "peat", is a product of the growth process of swamps and is formed in the quaternary stage by residues of swamp plants which cannot be completely decomposed and accumulated under the condition of abundant biogas. Contains a large amount of water and plant residues, humus and a part of minerals which are not completely decomposed. Kaolin is a non-metallic mineral, is a clay and claystone based on kaolinite group clay minerals, and is commercially available. Tapioca flour is starch obtained from tapioca or cassava plants, and is commercially available. The straw is a general term of stem and leaf (ear) parts of mature crops, and can be used after being crushed by straws of corn, wheat or rice. The seaweed extract fertilizer is a fertilizer-grade seaweed extract, is a pure natural marine organism product, and has all characteristics from the special seaweed itself and is purchased from the market.

The method has the advantages that the growth rule characteristics of the phoebe nankingense planted in different areas and environments are different, and the applicant finds that leaves planted in other places except the Zhejiang Huzhou area are thin in tips, have leaf ridges on two sides and are not smooth. Moreover, the change of conditions such as soil, climate, temperature and the like in the planting process of different areas can affect the chemical components in the planted machilus thunbergii, and the change of some conditions can even reduce the content of effective components such as crude sugar and the like by half, thereby greatly affecting the medicinal value of the machilus thunbergii.

In a preferred embodiment, the adjuvant is tetrahydropiperine.

The inventor finds that the phoebe nana fruit planted by the method provided by the invention is beneficial to reducing the blood sugar of a diabetic patient, and the infection caused by the disease such as bronchiectasis. After the obtained Machilus thunbergii fruit and leaves thereof are extracted together, the Machilus thunbergii extract has better effects of inhibiting the proliferation and differentiation of corresponding pathogenic bacteria, avoiding infection, reducing the blood glucose index and the like. During the extraction process, petroleum ether reflux is firstly carried out on the Machilus thunbergii fruits and leaves, some fatty esters and some sugar in the Machilus thunbergii fruits and leaves are removed, and when distilled water reflux extraction and ethanol microwave extraction are carried out once, the extracted components are more, and the extraction effect is better. A certain amount of tetrahydropiperine is added in the microwave extraction process of the ethanol, which is beneficial to the penetration of a solvent to the inside of the phoebe nanmu fruits and leaves, so that the internal effective components are dissolved out more fully. Furthermore, the inventors have unexpectedly found that when a system obtained by degreasing the fruits and leaves of the phoebe nanmu under reflux with petroleum ether, extracting the degreased fruit and leaves with distilled water under reflux, and extracting the distilled water with distilled water under reflux is directly subjected to microwave extraction with ethanol without filtration, the obtained extract has the best effect of inhibiting pathogenic bacteria such as aspergillus, and infection caused by pathogenic bacteria can be avoided. The extraction result is affected by adjusting the sequence of the extraction steps, omitting a certain extraction step, or changing without adding a certain component. The possible reason is that when the components degreased by petroleum ether are subjected to distilled water reflux extraction, substances which are beneficial to promoting certain fat-soluble or ethanol-soluble components are extracted, and under the action of the tetrahydropiperine, microwaves and other conditions, certain components which are difficult to permeate and damage in the leaves of the phoebe nanmu fruit and the phoebe nanmu can be dissolved out. Similarly, the leaves and fruits of Machilus thunbergii may contain different components, and these components may have synergistic effect in the extraction process to promote the dissolution of effective substances.

The Phoebe fruit planted by the method provided by the invention can avoid infection caused by scald, prostatitis, bronchiectasis and the like, inhibit the breeding and differentiation of some disease-treating bacteria and fungi in vivo, and is beneficial to reducing the blood glucose index of a diabetic patient.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

In addition, the raw materials used are commercially available from national chemical reagents, unless otherwise specified.

Examples

Example 1

Embodiment 1 provides a method for planting a machilus thunbergii, including the steps of:

(1) selecting yellow-heart soil with the pH value of 8 and a hilly land with the slope of 25 degrees of semiyin and semiyang;

(2) selecting seeds without diseases and insect pests and with full particles, sterilizing with 8wt% hydrogen peroxide solution for 15min, taking out, soaking seeds in 30 deg.C warm water for 3 hr, removing impurities on the seeds floating on water surface, taking out the seeds, and drying in the sun;

(3) planting the seeds and the safflower which are sterilized in the step (2) in the land selected in the step (1) in a sleeving manner, wherein the percentage of the plants of the phoebe-kanehira is 80% and the percentage of the safflower is 20% based on the total plants of the phoebe-kanehira and the safflower;

(4) applying natural fertilizers consisting of plant ash, rice hulls, peanut shells, grass carbon, kaolin, cassava powder, straws and seaweed extract fertilizers to the machilus thunbergii according to the weight of 700kg per mu of land respectively 20 days before the flowering period and 100 days after the flowering period, wherein the weight ratio of each component is 30: 10: 10: 25: 5: 5: 40: 5;

(5) the annual average temperature of the environment is 15 ℃; and (4) picking mature photinia fraseri fruits from planting to about 255 days.

In a second aspect of this embodiment, there is provided a method for extracting a photinia fraseri extract, including the steps of:

(1) taking the leaves and fruits of the phoebe nannieri provided by the embodiment, drying in the shade, wherein the weights of the leaves and fruits are respectively 3g and 10g, crushing and mixing;

(4) adding 120ml of 95wt% ethanol and 1g of tetrahydropiperine into the system in the step (3), extracting for 1 hour under the microwave power of 250W, standing for 10min, filtering, concentrating the filtrate, and drying to obtain the machilus thunbergii extract.

Example 2

Embodiment 2 provides a method for planting a machilus thunbergii, which includes the following steps:

(1) selecting yellow-heart soil with the pH value of about 7 and a hilly land with the slope of 25 degrees of semiyin and semiyang;

(4) applying natural fertilizers consisting of plant ash, rice hulls, peanut shells, grass carbon, kaolin, cassava powder, straws and seaweed extract fertilizers to the machilus thunbergii according to the weight of 800kg per mu of land respectively 20 days before the flowering period and 100 days after the flowering period, wherein the weight ratio of each component is 40: 15: 20: 40: 10: 10: 60: 8;

(4) adding 120ml of 95wt% ethanol and 3g of tetrahydropiperine into the system in the step (3), extracting for 1 hour under the microwave power of 250W, standing for 10min, filtering, concentrating the filtrate, and drying to obtain the machilus thunbergii extract.

Comparative example 1

Comparative example 1 provides a method for planting a machilus thunbergii, the method comprising the steps of:

(1) selecting yellow core soil with the pH value of about 8 and flat land with the gradient of less than 6 degrees;

In a second aspect of the present invention, there is provided a method for extracting a photinia fraseri extract, comprising the steps of:

Comparative example 2

Comparative example 2 provides a method for planting a machilus thunbergii, the method comprising the steps of:

(1) selecting yellow-heart soil with the pH value of 6 and a hilly land with the slope of 25 degrees of semiyin and semiyang;

Comparative example 3

Comparative example 3 provides a method for planting a machilus thunbergii, the method comprising the steps of:

(5) the average temperature of the environment is lower than 5 ℃ all year round; and (4) picking mature photinia fraseri fruits from planting to about 255 days.

Comparative example 4

Comparative example 4 provides a method for planting a machilus thunbergii, the method comprising the steps of:

(4) 700kg of urea is respectively applied to the machilus thunbergii per mu of land 20 days before the flowering period and 100 days after the flowering period;

Comparative example 5

Comparative example 5 provides a method for planting a machilus thunbergii, the method comprising the steps of:

(3) planting the sterilized seed grains in the step (2) in the place selected in the step (1);

Comparative example 6

Comparative example 6 provides a method of planting the machilus thunbergii, which is the same as in example 1.

This comparative example provides, in a second aspect, a method for extracting the extract of photinia fraseri, which is different from the extraction step of example 1 in that the step (2) is not performed, that is, the fruit and leaves of photinia fraseri are not subjected to petroleum ether reflux, but the extraction is directly started with distilled water.

Comparative example 7

Comparative example 7 provides a method of planting the machilus thunbergii, which is the same as in example 1.

The second aspect of the present comparative example provides the extraction method of the machilus thunbergii extract, which is different from the extraction step of example 1 in that the distilled water in the step (3) is heated and refluxed, and is cooled after being extracted for 3 hours, and is allowed to stand for 10min, and the filtrate is filtered, concentrated, and dried to obtain the machilus thunbergii extract (i.e., the step (4) is not performed, the ethanol microwave extraction is not performed on the system, and only the distilled water extraction is performed).

Comparative example 8

Comparative example 8 provides a method of planting the machilus thunbergii, which is the same as in example 1.

This comparative example provides, in a second aspect, a method for extracting the extract of photinia glabra, which differs from the extraction step of example 1 in that no tetrahydropiperine is added in the step (4).

Comparative example 9

Comparative example 9 provides a method of planting the machilus thunbergii, which is the same as in example 1.

A second aspect of the present comparative example provides the method for extracting a machilus thunbergii extract, which includes steps different from those of the extraction step in example 1, in that the step (3) is omitted, and the filter residue obtained by refluxing petroleum ether is directly subjected to microwave extraction with ethanol to obtain the machilus thunbergii extract.

Comparative example 10

Comparative example 10 provides a method of planting a machilus thunbergii, which is the same as in example 1.

The second aspect of the present comparative example provides the method for extracting the machilus thunbergii extract, which comprises the steps different from the extraction step of example 1, in that the filter residue obtained by refluxing the petroleum ether in the step (1) is subjected to microwave extraction with ethanol in the step (4), distilled water is added into the obtained system for extraction with distilled water, and then the machilus thunbergii extract is obtained after the steps of standing, filtering, drying and the like as described in example 1.

Comparative example 11

Comparative example 11 provides a method of planting the machilus thunbergii, which is the same as in example 1.

In the second aspect of this comparative example, the extraction method of the extract of photinia fraseri is provided, which differs from the extraction step of example 1 in that the microwave extraction is not performed in the step (4), and only the ethanol is heated and refluxed for extraction.

Comparative example 12

Comparative example 12 provides a method of planting a machilus thunbergii, which is the same as in example 1.

In the second aspect of this comparative example, the extraction method of the extract of machilus thunbergii is provided, which is different from the extraction step of example 1 in that only machilus thunbergii is extracted in the step (1), that is, only machilus thunbergii is extracted without leaves.

Comparative example 13

Comparative example 13 provides a method of planting a machilus thunbergii, which is the same as in example 1.

In a second aspect of the present comparative example, the extraction method of the extract of machilus thunbergii is provided, which is different from the extraction step of example 1 in that only machilus thunbergii leaves and no machilus thunbergii fruits are present in the step (1), that is, the machilus thunbergii leaves are separately extracted, and the extract does not contain machilus thunbergii fruits.

Evaluation of Performance

1. Survival and fruit yield statistics

The survival rate of the phoebe nanseri plants planted by the planting methods provided in the above examples and comparative examples was counted, and the yield of phoebe nanseri fruits was counted per mu of the survived plants, and the results are shown in the following table.

TABLE 1 survival and yield statistics

2. Bacteriostasis test

The extracts provided in the above examples and comparative examples were tested for inhibition of aspergillus, streptococcus pneumoniae, staphylococcus aureus, pseudomonas aeruginosa. Firstly, 0.5g of the extract is weighed and added into 15ml of 45 wt% ethanol solution, and the solution to be detected is obtained after the extract is dissolved. Then inoculating aspergillus, streptococcus pneumoniae, staphylococcus aureus and pseudomonas aeruginosa in a culture medium, culturing for 24 hours at 37 ℃ in a thermostat, adding a proper amount of distilled water, and shaking to obtain test bacterium suspension. 10ml of the melted LB medium was placed in a Mucun petri dish, and then 1ml of the test bacteria suspension was added dropwise to the dish and allowed to spread over the bottom of the dish. Then, 4 Oxford cups were placed on each plate, 0.1ml of the solution to be tested for the extract was added thereto, and the size of the zone diameter (unit: mm) was measured after culturing at 37 ℃ for 24 hours.

TABLE 2 results of the bacteriostatic test

3. Clinical trial

Several diabetics are randomly selected, 3g of the machilus thunbergii extract provided by the embodiment 1 of the invention is soaked in warm water for drinking every day, and fasting blood glucose index and 2-hour postprandial blood glucose index changes of the patients before eating the machilus thunbergii extract and after eating 4 weeks and 8 weeks respectively are counted.

Example 1: diabetic patients, kaixx, men, 58 years old, had diabetes for 3 years.

Example 2: diabetic patients, plum x, female, 38 years old, had diabetes for 1 year.

Example 3: diabetic patients, xiaxx, men, 46 years old, have had diabetes for 3 years.

Example 4: diabetic patients, xiaxx, women, 41 years old, have had diabetes for 2 years.

TABLE 3 blood sugar index table

As can be seen from the above tables 1 to 3, the survival rate of the phoebe nannie planted by the method provided by the invention is high, the survival rate of the phoebe nannie tree is high, the yield is high, and the yield is several times higher than that of the phoebe nannie tree planted by other methods. In addition, the Machilus thunbergii extract obtained by extracting the Machilus thunbergii fruits and leaves which are planted according to the method provided by the invention by adopting the extraction method provided by the invention can inhibit proliferation and differentiation of pathogenic bacteria, such as aspergillus, streptococcus pneumoniae, staphylococcus aureus and pseudomonas aeruginosa, which cause scald infection, bronchiectasis and infection and other diseases (such as prostatitis, diabetes, asthma and the like). And the obtained extract is taken by several diabetics for 8 weeks, so that the fasting blood sugar and the blood sugar 2 hours after the meal are obviously reduced, and the blood sugar index of the diabetics can be stabilized and controlled to a certain extent to control the disease condition.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. The method for planting the photinia fraseri is characterized by at least comprising the following steps:

(1) selecting soil with a pH value of 8-9 in a semi-negative and semi-positive land with a gradient of 15-30 degrees;

(2) selecting Machilus thunbergii seeds without diseases and insect pests, sterilizing with 8wt% hydrogen peroxide solution for 15min, taking out, and drying in the sun;

(4) applying a natural fertilizer to the phoebe zhennan;

(5) keeping the average ambient temperature at 14-16 ℃;

the soil is yellow core soil; the natural fertilizer comprises the following components in parts by weight: 30-40 parts of plant ash, 10-15 parts of rice hull, 10-20 parts of peanut shell, 25-40 parts of grass carbon, 5-10 parts of kaolin, 5-10 parts of cassava flour, 40-60 parts of straw and 5-8 parts of seaweed extract fertilizer;

the fertilizing time of the natural fertilizer is 20 days before the flowering period and 100-110 days after the flowering.

2. The method for planting phoebe nanseris according to claim 1, wherein the percentage of the plants of phoebe nanseris is 80-85% based on the total plants of phoebe nanseris and safflower.

3. The method for planting the photinia fraseri of claim 1, wherein the amount of the natural fertilizer is 600-800 kg/mu.

4. The method for extracting the photinia fraseri extract obtained by the planting method of claims 1 to 3, wherein the method for extracting the photinia fraseri extract comprises the following steps:

(4) adding 120ml of 95wt% ethanol and 1-3 g of an auxiliary agent into the system in the step (3), extracting for 1 hour under the microwave power of 250W, standing for 10min, filtering, concentrating the filtrate, and drying to obtain the machilus thunbergii extract; the auxiliary agent is tetrahydropiperine.