CN108264999A - A kind of anaerobic type faecal microbiota extraction element - Google Patents

A kind of anaerobic type faecal microbiota extraction element Download PDF

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Publication number
CN108264999A
CN108264999A CN201810215284.4A CN201810215284A CN108264999A CN 108264999 A CN108264999 A CN 108264999A CN 201810215284 A CN201810215284 A CN 201810215284A CN 108264999 A CN108264999 A CN 108264999A
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sealing cover
annular
side wall
collecting
broken
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CN108264999B (en
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不公告发明人
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Zhang Wen
Zhang Yi
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Yantai Minwang Electromechanical Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • C12M45/02Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02Separating microorganisms from the culture medium; Concentration of biomass
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/12Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Mechanical Engineering (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Filtration Of Liquid (AREA)

Abstract

The invention discloses a kind of anaerobic type faecal microbiota extraction element, extraction element includes sample collecting device, collecting bag, breaker and filter device;Sample collecting device includes the cylindrical collection container of upper end opening and is arranged on the upper cover of collection container upper surface, and circular through hole or ellipse hole are provided on upper lid, and back taper collecting hood, collecting hood lower port setting collecting bag are provided with below upper cover;Collecting bag is provided with seal assembly on the side wall of opening, and seal assembly can be sleeved in the lower port of collecting hood;Dilution is held in collection container.The beneficial effects of the invention are as follows:The present invention is not only easy to operate, but also combines plastic sealing bag and acquire fecal sample, as far as possible time of contact of fecal sample and air less, improves caprophyl survival rate and therapeutic effect.

Description

A kind of anaerobic type faecal microbiota extraction element
This case is divisional application, original application it is entitled:A kind of intestinal contents flora extraction element, original application The applying date is:2016-04-27, original application application No. is:201610269238.3.
Technical field
The present invention relates to a kind of medical extraction element more particularly to a kind of anaerobic type faecal microbiota extraction elements.
Background technology
Excrement transplanting be it is a kind of treat the method for disease by rebuilding intestinal flora, i.e., will the excrement of Healthy People by Obtain beneficial to caprophyl liquid, that is, removing the parasites such as worm's ovum and only retaining useful flora after reason, be then fabricated to capsulae enterosolubilis or it Between take orally.Medical worker is either still psychological beyond affordability from vision, smell when preparing stool suspension, and thinks Extraction process must be rapidly completed by ensureing the activity of caprophyl, since the flora overwhelming majority in enteron aisle is anaerobic bacterium, in medical matters When personnel take stool sampl, the time of stool sampl one section of ingress of air, nothing is also difficult to guarantee very much in extraction process The condition of oxygen, therefore current extraction equipment is it is difficult to ensure that the quality of caprophyl.
Invention content
The present invention provides a kind of time of contact for reducing excrement and air as far as possible, easy to operate and raising caprophyls to survive The intestinal contents flora extraction element of rate.
The present invention is realized by following measure:A kind of intestinal contents flora extraction element, including breaker, with The filter device of the breaker connection and the negative pressure device being connect with the filter device;
The breaker includes the end of the broken bucket with sealing cover, the upper surface of the broken bucket and the sealing cover port Annular groove is both provided on face, the circular blade being used cooperatively is provided in two annular grooves;The sealing bezel ring, The madial wall height of connected in star is less than the lateral wall of the sealing cover annular groove;
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, positioned at institute It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken Barrel side wall is provided with handle;
It is provided on the sealing cover with leachy circular groove, is provided in the circular groove and is hinged with the sealing cover Air valve cap;
The dilution is filled in the broken bucket;The liquid outlet with switch valve is provided on the broken bucket lower sides.
The filter device includes the filtering container of sealing, and inlet is provided in the upper portion side wall of the filtering container, The inlet is connect with the liquid outlet of the breaker, and liquid outlet, position are provided on the lower sides of the filtering container Described filter is provided with negative pressure ventilation mouth, the inlet and the negative pressure ventilation mouth on container side wall above the liquid outlet Between it is described filtering container in be provided with several grades filter membrane that from top to bottom aperture reduces step by step, filter membrane described in adjacent two-stage Between have an interval, be provided with the drain with sealing cover on the side wall of the filtering container between filter membrane described in adjacent two-stage Mouthful;
It is described filtering container upper surface be provided with leachy circular groove, be provided in the circular groove with it is described Filter the hinged air valve cap in container upper end face.
The height of the circular blade is less than the lateral wall height of the annular groove and the madial wall of the annular groove Highly.
The sealing cover of the breaker is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, described Broken bucket is provided with the external screw thread being connect with the internal thread.
Several L-type notches are evenly arranged on the side wall of the broken bucket on the outside of the annular groove, positioned at institute The wall outer surface for stating the sealing cover on the outside of annular groove is provided with the positioning column being used cooperatively with the L-type notch.
Preferably, the present invention provides a kind of intestinal contents flora extraction element, including breaker and breaker The extraction tube of connection and the negative pressure device being connect with the extraction tube;The breaker includes the broken bucket with sealing cover, Annular groove is both provided on the end face of the upper surface of the broken bucket and the sealing cover port, in two annular grooves It is provided with the circular blade being used cooperatively;The madial wall height of the sealing cover annular groove is less than the sealing cover annular groove Lateral wall;
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, positioned at institute It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken Barrel side wall is provided with handle;
It is provided on the sealing cover with leachy circular groove, is provided in the circular groove and is hinged with the sealing cover Air valve cap;
The dilution is filled in the broken bucket;The liquid outlet with switch valve is provided on the broken bucket lower sides.
The height of the circular blade is less than the lateral wall height of the annular groove.
The sealing cover is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, the broken bucket setting There is the external screw thread being connect with the internal thread;Or, L-type is provided on the side wall of the broken bucket on the outside of the annular groove Notch, the wall outer surface of the sealing cover is provided with what is be used cooperatively with the L-type notch on the outside of the annular groove Positioning column.
The extraction tube includes the filter membrane that tube body and several grades are connect with the inner wall sealing of the tube body, on the tube body End is provided with inlet, and the bottom of the pipe is provided with the negative pressure ventilation mouth being connect with negative pressure device, close to the bottom of the pipe Sealed set has a hard liquid storage box connected with the tube body on the side wall of the tube body, the filter membrane from top to bottom aperture In the trend reduced step by step.
The material of the tube body is silica gel, soft rubber, PVC or flexible plastic, and is integrally formed;The aperture of the filter membrane is 0.0004-5.00mm。
Preferably, the present invention also provides a kind of intestinal contents flora extraction element, the extraction element includes sample Harvester and collecting bag;The sample collecting device includes the cylindrical collection container of upper end opening and is arranged on the acquisition The upper cover in container upper end face is provided with circular through hole or ellipse hole on the upper lid, is provided with down below the upper cover Cone-shaped collection cover, the collecting hood lower port set the collecting bag;
The collecting bag is provided with seal assembly on the side wall of opening, and the seal assembly can be sleeved on the collecting hood In lower port;
Dilution is held in the collection container.
The seal assembly is magnetic stripe, and the collecting bag is symmetrical arranged on the side wall of opening there are two the magnetic stripe, The both ends articulated connection of two magnetic stripes.
The upper surface of the collection container bends inwards to form annular stop plate, and the annular stop plate upper surface is provided with Annular groove, the upper cover lower face are provided with the annular protrusion being used cooperatively with the annular groove.
Taking mouth is provided on the side wall of the collection container, is hinged on the side wall of the collection container and takes object with described Mouth is corresponding to take object door.
Magnetic stripe one end of the collecting bag is connected with drawstring.
Preferably, invention further provides a kind of intestinal contents flora extraction element, the extraction element includes sample Harvester and collecting bag;The sample collecting device includes the cylindrical collection container of upper end opening and is arranged on the acquisition The upper cover in container upper end face is provided with circular through hole or ellipse hole on the upper lid, is provided with down below the upper cover Cone-shaped collection cover, the collecting hood lower port set the collecting bag;
The collecting bag is provided with seal assembly on the side wall of opening, and the seal assembly can be sleeved on the collecting hood In lower port;
Dilution is held in the collection container.
The seal assembly is magnetic stripe, and the collecting bag is symmetrical arranged on the side wall of opening there are two the magnetic stripe, The both ends articulated connection of two magnetic stripes.
The upper surface of the collection container bends inwards to form annular stop plate, and the annular stop plate upper surface is provided with Annular groove, the upper cover lower face are provided with the annular protrusion being used cooperatively with the annular groove.
Taking mouth is provided on the side wall of the collection container, is hinged on the side wall of the collection container and takes object with described Mouth is corresponding to take object door.
Magnetic stripe one end of the collecting bag is connected with drawstring.
The extraction element further includes breaker, and the breaker includes the broken bucket with sealing cover, described broken Annular groove is both provided on the end face of the upper surface of broken bucket and the sealing cover port, is provided in two annular grooves The circular blade being used cooperatively;The madial wall height of the sealing cover annular groove is less than the outside of the sealing cover annular groove Wall;
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, positioned at institute It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken Barrel side wall is provided with handle;
It is provided on the sealing cover with leachy circular groove, is provided in the circular groove and is hinged with the sealing cover Air valve cap;
The dilution is filled in the broken bucket;The liquid outlet with switch valve is provided on the broken bucket lower sides.
The height of the circular blade is less than the lateral wall height of the annular groove.
The sealing cover is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, the broken bucket setting There is the external screw thread being connect with the internal thread;Or, L-type is provided on the side wall of the broken bucket on the outside of the annular groove Notch, the wall outer surface of the sealing cover is provided with what is be used cooperatively with the L-type notch on the outside of the annular groove Positioning column.
The extraction element further includes filter device, and the filter device includes into fluid cylinder, several intermediate cylinders and goes out liquid Tin, it is both provided with cambered surface filter screen in the intermediate cylinder and the discharge barrel;The intermediate cylinder close to upper port madial wall and It is described go out fluid cylinder on the inner wall of upper port on be provided with annular gear edge, the cambered surface filter screen include annular backup pad and The cambered surface supporting network of the annular backup pad lower face is arranged on, is provided with filter membrane in the mesh of the cambered surface supporting network, institute Annular backup pad load is stated on the annular backup pad;
The intermediate cylinder upper port is provided with internal thread, and the intermediate cylinder lower port is provided with external screw thread, described into fluid cylinder lower end Mouth is provided with the external screw thread used with the intermediate cylinder screw-internal thread fit, it is described go out fluid cylinder upper port be provided with and the centre The internal thread that cylinder screw-internal thread fit uses;Several described intermediate cylinders are connected through a screw thread;
It is described go out fluid cylinder lower end be provided with liquid outlet, it is described go out fluid cylinder side wall on be provided with negative pressure ventilation mouth;
It is described into being provided on fluid cylinder upper surface with leachy circular groove, be provided with and the sealing in the circular groove Cover hinged air valve cap.
Several flexible cleaning balls are placed in the cambered surface filter screen.
The beneficial effects of the invention are as follows:The present invention is not only easy to operate, but also combines plastic sealing bag and acquire fecal sample, Lack the time of contact of fecal sample and air as far as possible, improve caprophyl survival rate and therapeutic effect;It in use, at the first time will be strong The fecal sample of health people is put into plastic sealing bag, keep excrement be in oxygen-free environment, then hermetic bag is placed on fill with it is dilute It releases on the upper surface of broken bucket of liquid, then covers sealing cover, sealing cover moves downward, circular blade and sealing when broken bucket The circular blade of lid can cut in half hermetic bag when being in contact, and the half containing fecal sample is automatically fallen into broken bucket, Crank handle drives shaft to rotate, and the spine protrusion in shaft is relatively moved with the spine protrusion on filter screen, hermetic bag It is scratched rapidly, excrement obtains the fecal liquid of removal large granular impurity, open liquid outlet through diluting and passing through filter screen filtration Switch valve, fecal liquid is discharged in the filter device that negative pressure vacuumizes, after fecal liquid fully enters filter device, Valve is turned off the switch, opens the air valve cap in filtering container upper end face, fecal liquid finally obtains intestinal beneficial after multistage filtering Flora.
Description of the drawings
Fig. 1 is the structure diagram of the embodiment of the present invention 1.
Fig. 2 is the structure diagram that bucket is crushed in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 3 is the structure diagram of breaker sealing cover in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 4 is another structure diagram that bucket is crushed in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 5 is the structure diagram of filter device in the embodiment of the present invention 1.
Fig. 6 is the structure diagram of air valve cap in the embodiment of the present invention 1.
Fig. 7 is the structure diagram of the embodiment of the present invention 2.
Fig. 8 is the structure diagram of filter device in the embodiment of the present invention 2.
Fig. 9 is the structure diagram of harvester in the embodiment of the present invention 3 and embodiment 4.
Figure 10 is the structure diagram of collecting bag in the embodiment of the present invention 3 and embodiment 4.
Figure 11 is the schematic diagram of harvester use state in the embodiment of the present invention 3 and embodiment 4.
Figure 12 is the structure diagram of harvester and filter device in the embodiment of the present invention 4.
Figure 13 is the structure diagram of filter device in the embodiment of the present invention 4.
In figure, 1, broken bucket;2nd, filter screen;3rd, bearing;4th, shaft;5th, spine protrusion;6th, sealing cover;7th, air valve cap;8、 Handle;9th, liquid outlet;10th, connecting tube;11st, inlet;12nd, container is filtered;13rd, negative pressure ventilation mouth;14th, liquid outlet;15th, outer spiral shell Line;16th, the lateral wall of annular groove;17th, circular blade;18th, annular groove madial wall;19th, L-type notch;20th, filter membrane;21、 Leakage fluid dram;22nd, stomata;23rd, air valve cap;24th, seal protuberance;25th, extraction tube;26th, hard liquid storage box;27th, collection container;28、 Dilution;29th, collecting bag;30th, collecting hood;31st, annular protrusion;32nd, annular stop plate;33rd, upper cover;34th, taking mouth;35th, it draws Rope;36th, articulated shaft;37th, magnetic stripe;38th, collecting bag upper edge;39th, it latches;40th, ellipse hole;41st, object door is taken;42nd, into fluid cylinder; 43rd, intermediate cylinder;44th, go out fluid cylinder;45th, annular gear edge;46th, annular backup pad;47th, flexible cleaning ball;48th, cambered surface supporting network.
Specific embodiment
In order to clarify the technical characteristics of the invention, below by specific embodiment, this programme is illustrated.
Embodiment 1
Referring to Fig. 1-Fig. 6, a kind of intestinal contents flora extraction element, the filtering being connect including breaker, with breaker Device and the negative pressure device being connect with filter device;
Breaker includes the broken bucket 1 with sealing cover 6, is all provided on the end face of 6 port of upper surface and sealing cover of broken bucket 1 Annular groove is equipped with, the circular blade 17 being used cooperatively is provided in two annular grooves, i.e., in broken 1 upper surface of bucket and is sealed The circular blade 17 being used cooperatively is provided in the port end face of lid 6, plays the role of cut-out;6 annular groove of sealing cover it is interior 18 height of side wall is less than the lateral wall 16 of 6 annular groove of sealing cover, and the cut-off parts inside broken bucket 1 can smoothly fall into broken In bucket 1;The height of circular blade 17 is less than 16 height of lateral wall of annular groove and 18 height of madial wall of annular groove.
A filter screen 2 is provided in 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine protrusion 5, was located at The shaft 4 for being covered with spine protrusion 5 is provided on 2 top 1 side wall of broken bucket of strainer, the both ends of shaft 4 are horizontally disposed by bearing 3 On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by broken 1 side wall of bucket and is provided with handle 8;
The circular groove with stomata 22 is provided on sealing cover 6, the air valve cap hinged with sealing cover 6 is provided in circular groove 7(Or 23 in figure), air valve cap 7(Or 23 in figure)On be provided with and 22 corresponding seal protuberance 24 of stomata;
Dilution 28 is filled in broken bucket 1;The liquid outlet 14 with switch valve is provided on 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with The external screw thread 15 of internal thread connection;Alternatively, several L-types are evenly arranged on the side wall of broken bucket 1 on the outside of annular groove Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove Column.
Filter device includes the filtering container 12 of sealing, filters and is provided with inlet 11 in the upper portion side wall of container 12, into Liquid mouth 11 is connect by connecting tube 10 with the liquid outlet 9 of breaker, is filtered and is provided with liquid outlet on the lower sides of container 12 14, it is filtered above liquid outlet 14 on 12 side wall of container and is provided with negative pressure ventilation mouth 13, inlet 11 and negative pressure ventilation mouth 13 Between be provided with several grades filter membrane 20 that from top to bottom aperture reduces step by step in filtering container 12, adjacent double-filtration film 20 it Between have an interval, be provided with the leakage fluid dram 21 with sealing cover on the side wall of filtering container 12 between adjacent double-filtration film 20;
The upper surface of filtering container 12 is provided with the circular groove with stomata 22, is provided in circular groove and filtering container 12 The hinged air valve cap 23 in upper surface is provided with and 22 corresponding seal protuberance 24 of stomata on air valve cap 23.Wherein, negative pressure device is The prior art, details are not described herein.
In use, the fecal sample of Healthy People is put into plastic sealing bag at the first time, excrement is kept to be in anaerobic ring In border, then hermetic bag is placed on the upper surface for the broken bucket 1 for filling with dilution 28, then covers sealing cover 6, sealing cover 6 It moves downward, hermetic bag can be cut in half when the circular blade of circular blade and sealing cover 6 for crushing bucket 1 is in contact, Half containing fecal sample is automatically fallen into broken bucket 1, and crank handle 8 drives shaft 4 to rotate, the spine protrusion 5 in shaft 4 It is relatively moved with the spine protrusion 5 on filter screen 2, hermetic bag is scratched rapidly, and excrement is through diluting and passing through 2 mistake of filter screen Filter obtains the fecal liquid of removal large granular impurity, opens the switch valve of liquid outlet, fecal liquid is discharged to negative pressure and is vacuumized Filter device in, after fecal liquid fully enters filter device, turn off the switch valve, open the gas of 12 upper surface of filtering container Valve deck 23, fecal liquid finally obtain intestinal beneficial flora after multistage filtering.
Embodiment 2
Referring to Fig. 2, Fig. 3, Fig. 4, Fig. 7 and Fig. 8, the present invention provides a kind of intestinal contents flora extraction element, including broken Device, the extraction tube 25 being connect with breaker and the negative pressure device being connect with extraction tube 25;Breaker is included with sealing The broken bucket 1 of lid 6 is both provided with annular groove on the end face of 6 port of upper surface and sealing cover of broken bucket 1, and two annulars are recessed The circular blade 17 being used cooperatively is provided in slot, i.e., is provided in broken 1 upper surface of bucket and in the port end face of sealing cover 6 and matches The circular blade 17 used is closed, plays the role of cut-out;18 height of madial wall of 6 annular groove of sealing cover is less than 6 ring of sealing cover The lateral wall 16 of connected in star, crushing the cut-off parts inside bucket 1 can smoothly fall into broken bucket 1;The height of circular blade 17 Less than 16 height of lateral wall of annular groove and 18 height of madial wall of annular groove.
A filter screen 2 is provided in 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine protrusion 5, was located at The shaft 4 for being covered with spine protrusion 5 is provided on 2 top 1 side wall of broken bucket of strainer, the both ends of shaft 4 are horizontally disposed by bearing 3 On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by broken 1 side wall of bucket and is provided with handle 8;
The circular groove with stomata 22 is provided on sealing cover 6, the air valve cap hinged with sealing cover 6 is provided in circular groove 7(Or 23 in figure), air valve cap 7(Or 23 in figure)On be provided with and 22 corresponding seal protuberance 24 of stomata;
Dilution 28 is filled in broken bucket 1;The liquid outlet 14 with switch valve is provided on 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with The external screw thread 15 of internal thread connection;Alternatively, several L-types are evenly arranged on the side wall of broken bucket 1 on the outside of annular groove Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove Column.
Extraction tube 25 includes the filter membrane 20 that tube body and the inner wall sealing of several grades and tube body connect, and tube body upper end is provided with Inlet 11, the liquid outlet 9 of breaker are connect with inlet 11, and bottom of the pipe is provided with the negative pressure being connect with negative pressure device and takes out Gas port 13, sealed set has a hard liquid storage box 26 connected with tube body, filter membrane 20 on the side wall of bottom of the pipe tube body From top to bottom aperture is in the trend reduced step by step.
The material of tube body is silica gel, soft rubber, PVC or flexible plastic, and the material of 26 box of hard liquid storage is rigid plastics, hard The upper port of liquid storage box 26 is integrally formed with tube body;The aperture of filter membrane 20 is 0.0004-5.00mm.
In use, the fecal sample of Healthy People is put into plastic sealing bag at the first time, excrement is kept to be in anaerobic ring In border, then hermetic bag is placed on the upper surface for the broken bucket 1 for filling with dilution 28, then covers sealing cover 6, sealing cover 6 It moves downward, hermetic bag can be cut in half when the circular blade of circular blade and sealing cover 6 for crushing bucket 1 is in contact, Half containing fecal sample is automatically fallen into broken bucket 1, and crank handle 8 drives shaft 4 to rotate, the spine protrusion 5 in shaft 4 It is relatively moved with the spine protrusion 5 on filter screen 2, hermetic bag is scratched rapidly, and excrement is through diluting and passing through 2 mistake of filter screen Filter obtains the fecal liquid of removal large granular impurity, opens the switch valve of liquid outlet, fecal liquid is discharged to negative pressure and is vacuumized Filter device in, after fecal liquid fully enters filter device, turn off the switch valve, fecal liquid after multistage filtering most Intestinal beneficial flora is obtained eventually.
Embodiment 3
Referring to Fig. 9, Figure 10 and Figure 11, an embodiment of the present invention provides a kind of intestinal contents flora extraction element, extraction elements Including sample collecting device and collecting bag 29;Sample collecting device includes the cylindrical collection container 27 of upper end opening and is arranged on The upper cover 33 of 27 upper surface of collection container is provided with circular through hole or ellipse hole 40 in upper cover 33, and the lower section of upper cover 33 is set It is equipped with back taper collecting hood 30,30 lower port of collecting hood setting collecting bag 29;
Collecting bag 29 is close to opening(Collecting bag upper edge 38)Side wall on be provided with seal assembly, seal assembly can be sleeved on collection In the lower port of cover 30;Dilution 28 is held in collection container.
Seal assembly is magnetic stripe 37, and collecting bag 29 is arranged symmetrically on the side wall of opening, and there are two magnetic stripe 37, two magnetic The both ends of item 37 are articulated and connected by articulated shaft 36.
The upper surface of collection container 27 bends inwards to form annular stop plate 32, and 32 upper surface of annular stop plate is provided with ring Connected in star, 33 lower face of upper cover are provided with the annular protrusion 31 being used cooperatively with annular groove.
Taking mouth 34 is provided on the side wall of collection container 27, it is right with taking mouth 34 to be hinged on the side wall of collection container 27 That answers takes object door 41.Graduation mark is provided on the inner wall of collection container 27, ensures the liquid level just contact sleeve of dilution 28 Collecting bag 29 on collecting hood 30, magnetic stripe 37 enable to collecting bag to be just sleeved on collecting hood 39 and do not fall just, when When having the excrement to enter collecting bag, gravity can cause collecting bag 39 to move downward.
37 one end of magnetic stripe of collecting bag 29 is connected with drawstring 35 by lock 39.
In use, dilution 28 will be held to graduation mark in collection container 27,33 side of upper cover for packaging collecting bag 29 is existed On collection container 27, the health worker of sample to be collected is sitting in upper cover 33, when there is excrement to enter collecting bag, collecting bag due to Gravity increase can cause collecting bag 39 to move downward, and when being detached from collecting hood 30, magnetic stripe 37 attracts rapidly and in the pressure of dilution The gas in collecting bag is excluded under power as possible, improves the anaerobic quality in collecting bag 39.
Embodiment 4
It is described an embodiment of the present invention provides a kind of intestinal contents flora extraction element referring to Fig. 2-Fig. 4 and Fig. 9-Figure 13 Extraction element includes sample collecting device and collecting bag 29;Sample collecting device includes the cylindrical collection container 27 of upper end opening With the upper cover 33 for being arranged on 27 upper surface of collection container, circular through hole or ellipse hole 40, upper cover 33 are provided in upper cover 33 Lower section be provided with back taper collecting hood 30,30 lower port of collecting hood setting collecting bag 29;
Collecting bag 29 is provided with seal assembly on the side wall of opening, and seal assembly can be sleeved on the lower port of collecting hood 30 On;Dilution 28 is held in collection container.
Seal assembly is magnetic stripe 37, and collecting bag 29 is arranged symmetrically on the side wall of opening, and there are two magnetic stripe 37, two magnetic The both ends articulated connection of item 37.
The upper surface of collection container 27 bends inwards to form annular stop plate 32, and 32 upper surface of annular stop plate is provided with ring Connected in star, 33 lower face of upper cover are provided with the annular protrusion 31 being used cooperatively with annular groove.
Taking mouth 34 is provided on the side wall of collection container 27, it is right with taking mouth 34 to be hinged on the side wall of collection container 27 That answers takes object door 41.
37 one end of magnetic stripe of collecting bag 29 is connected with drawstring 35 by lock 39.
The extraction element further includes breaker, and breaker includes the broken bucket 1 with sealing cover 6, crushes bucket 1 Annular groove is both provided on the end face of 6 port of upper surface and sealing cover, the ring being used cooperatively is provided in two annular grooves Shape blade 17 crushes in 1 upper surface of bucket and is provided with the circular blade 17 being used cooperatively in the port end face of sealing cover 6, rises To the effect of cut-out 29 seal of collecting bag;18 height of madial wall of 6 annular groove of sealing cover is less than 6 annular groove of sealing cover Lateral wall 16, crushing the cut-out collecting bag inside bucket 1 can smoothly fall into broken bucket 1;The height of circular blade 17 is less than ring 16 height of lateral wall of connected in star and 18 height of madial wall of annular groove.
A filter screen 2 is provided in 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine protrusion 5, was located at The shaft 4 for being covered with spine protrusion 5 is provided on 2 top 1 side wall of broken bucket of strainer, the both ends of shaft 4 are horizontally disposed by bearing 3 On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by broken 1 side wall of bucket and is provided with handle 8;
The circular groove with stomata 22 is provided on sealing cover 6, the air valve cap hinged with sealing cover 6 is provided in circular groove 7(Or 23 in figure), air valve cap 7(Or 23 in figure)On be provided with and 22 corresponding seal protuberance 24 of stomata;
Dilution 28 is filled in broken bucket 1;The liquid outlet 14 with switch valve is provided on 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with The external screw thread 15 of internal thread connection;Alternatively, several L-types are evenly arranged on the side wall of broken bucket 1 on the outside of annular groove Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove Column.
The extraction element further includes filter device, and the filter device is included into fluid cylinder 42,43 and of several intermediate cylinders Go out fluid cylinder 44, cambered surface filter screen is both provided in intermediate cylinder 43 and discharge barrel 44;Intermediate cylinder 43 close to upper port madial wall and Go out fluid cylinder 44 on the inner wall of upper port on be provided with annular gear along 45, cambered surface filter screen includes annular backup pad 46 and sets The cambered surface supporting network 48 in 46 lower face of annular backup pad is put, filter membrane, annular branch are provided in the mesh of cambered surface supporting network 48 Fagging 46, which is carried on a shoulder pole to keep off along 45 in annular and pass through adjacent 43 lower port of intermediate cylinder in top, is pressed on annular gear along 45;
43 upper port of intermediate cylinder is provided with internal thread, and 43 lower port of intermediate cylinder is provided with external screw thread, is set into 42 lower port of fluid cylinder There is the external screw thread used with 43 screw-internal thread fit of intermediate cylinder, the upper port for going out fluid cylinder 44 is provided with and 43 screw-internal thread fit of intermediate cylinder The external screw thread used;Several intermediate cylinders 43 are connected through a screw thread;
Go out 44 lower end of fluid cylinder and be provided with liquid outlet 14, go out on the side wall of fluid cylinder 44 to be provided with negative pressure ventilation mouth 13;
It is provided on into 42 upper surface of fluid cylinder with leachy circular groove, the gas hinged with sealing cover is provided in circular groove Valve deck;Or into fluid cylinder and to go out the side wall of fluid cylinder and intermediate cylinder be soft material, such as silica gel, soft rubber, PVC or flexible plastic. Several flexible cleaning balls 47 are placed in cambered surface filter screen, flexible cleaning ball 47 is covered with the sphere of thorn for softwood matter and surface, soft Property rotating wash bowl 47 be the prior art, details are not described herein.
In use, dilution 28 will be held to graduation mark in collection container 27,33 side of upper cover for packaging collecting bag 29 is existed On collection container 27, the health worker of sample to be collected is sitting in upper cover 33, when there is excrement to enter collecting bag, collecting bag due to Gravity increase can cause collecting bag 39 to move downward, and when being detached from collecting hood 30, magnetic stripe 37 attracts rapidly and in the pressure of dilution The gas in collecting bag is excluded under power as possible, improves the anaerobic quality in collecting bag 39.Then, hermetic bag is placed on fill with it is dilute It releases on the upper surface of broken bucket 1 of liquid 28, then covers sealing cover 6, sealing cover 6 moves downward, when the circular blade of broken bucket 1 Hermetic bag can be cut in half when being in contact with the circular blade of sealing cover 6, the half containing fecal sample is automatically fallen into brokenly In broken bucket 1, crank handle 8 drives shaft 4 to rotate, and with the spine protrusion 5 on filter screen 2 phase occurs for the spine protrusion 5 in shaft 4 To movement, hermetic bag is scratched rapidly, and excrement is filtered through diluting and passing through filter screen 2, obtains the excrement of removal large granular impurity Liquid opens the switch valve of liquid outlet, fecal liquid is discharged in the filter device that negative pressure vacuumizes, and treats fecal liquid whole Into after filter device, valve is turned off the switch, fecal liquid finally obtains intestinal beneficial flora after multistage filtering.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.

Claims (10)

1. a kind of anaerobic type faecal microbiota extraction element, which is characterized in that the extraction element includes sample collecting device, collects Bag, breaker and filter device;The sample collecting device includes the cylindrical collection container of upper end opening and is arranged on institute The upper cover of collection container upper surface is stated, circular through hole or ellipse hole are provided on the upper lid, is set below the upper cover Back taper collecting hood is equipped with, the collecting hood lower port sets the collecting bag;
The collecting bag is provided with seal assembly on the side wall of opening, and the seal assembly can be sleeved on the collecting hood In lower port;
Dilution is held in the collection container.
2. anaerobic type faecal microbiota extraction element according to claim 1, which is characterized in that the seal assembly is magnetic Item, the collecting bag is arranged symmetrically on the side wall of opening, and there are two the magnetic stripes, and the both ends of two magnetic stripes hingedly connect It connects.
3. anaerobic type faecal microbiota extraction element according to claim 1 or 2, which is characterized in that the collection container Upper surface bends inwards to form annular stop plate, and the annular stop plate upper surface is provided with annular groove, the upper cover lower end Face is provided with the annular protrusion being used cooperatively with the annular groove.
4. according to claim 1-3 any one of them anaerobic type faecal microbiota extraction elements, which is characterized in that the acquisition is held Taking mouth is provided on the side wall of device, is hinged on the side wall of the collection container and corresponding with the taking mouth takes object door.
5. according to claim 1-4 any one of them anaerobic type faecal microbiota extraction elements, which is characterized in that the collecting bag Magnetic stripe one end be connected with drawstring.
6. according to claim 1-5 any one of them anaerobic type faecal microbiota extraction elements, which is characterized in that the extraction dress It puts and further includes breaker, the breaker includes the broken bucket with sealing cover, the upper surface of the broken bucket and described Annular groove is both provided on the end face of sealing cover port, the endless knife being used cooperatively is provided in two annular grooves Sword;The madial wall height of the sealing cover annular groove is less than the lateral wall of the sealing cover annular groove;
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, positioned at institute It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken Barrel side wall is provided with handle;
The dilution is filled in the broken bucket;The liquid outlet with switch valve is provided on the broken bucket lower sides.
7. according to claim 1-6 any one of them anaerobic type faecal microbiota extraction elements, which is characterized in that the endless knife The height of sword is less than the lateral wall height of the annular groove.
8. according to claim 1-7 any one of them anaerobic type faecal microbiota extraction elements, which is characterized in that the sealing cover It is threadedly coupled with the broken bucket, the sealing cover is provided with internal thread, and the broken bucket is provided with to be connect with the internal thread External screw thread;Or, L-type notch is provided on the side wall of the broken bucket on the outside of the annular groove, positioned at the annular The wall outer surface of the sealing cover is provided with the positioning column being used cooperatively with the L-type notch on the outside of groove.
9. according to claim 1-8 any one of them anaerobic type faecal microbiota extraction elements, which is characterized in that the extraction dress It puts and further includes filter device, the filter device includes into fluid cylinder, several intermediate cylinders and going out fluid cylinder, the intermediate cylinder and described Cambered surface filter screen is both provided in discharge barrel;The intermediate cylinder close to upper port madial wall and it is described go out fluid cylinder close to upper port Inner wall on be provided with annular gear edge, the cambered surface filter screen includes annular backup pad and being arranged on the annular backup pad The cambered surface supporting network of lower face is provided with filter membrane in the mesh of the cambered surface supporting network, and the annular backup pad load is described On annular backup pad;
The intermediate cylinder upper port is provided with internal thread, and the intermediate cylinder lower port is provided with external screw thread, described into fluid cylinder lower end Mouth is provided with the external screw thread used with the intermediate cylinder screw-internal thread fit, it is described go out fluid cylinder upper port be provided with and the centre The internal thread that cylinder screw-internal thread fit uses;Several described intermediate cylinders are connected through a screw thread;
It is described go out fluid cylinder lower end be provided with liquid outlet, it is described go out fluid cylinder side wall on be provided with negative pressure ventilation mouth.
10. anaerobic type faecal microbiota extraction element according to claim 9, which is characterized in that in the cambered surface filter screen Place several flexible cleaning balls.
CN201810215284.4A 2016-04-27 2016-04-27 Anaerobic fecal flora extraction device Active CN108264999B (en)

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CN201810215282.5A Active CN108441412B (en) 2016-04-27 2016-04-27 Anaerobic collection and crushing device for fecal strain extraction
CN201810214744.1A Expired - Fee Related CN108485938B (en) 2016-04-27 2016-04-27 Anaerobic collection device and collection method for fecal strain extraction
CN201810215283.XA Expired - Fee Related CN108251288B (en) 2016-04-27 2016-04-27 Anaerobic fecal bacteria extraction device and method
CN201810213341.5A Expired - Fee Related CN108330057B (en) 2016-04-27 2016-04-27 Intestinal content flora extraction device and extraction method
CN201810214745.6A Expired - Fee Related CN108467827B (en) 2016-04-27 2016-04-27 Fecal flora extraction device
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CN201810215282.5A Active CN108441412B (en) 2016-04-27 2016-04-27 Anaerobic collection and crushing device for fecal strain extraction
CN201810214744.1A Expired - Fee Related CN108485938B (en) 2016-04-27 2016-04-27 Anaerobic collection device and collection method for fecal strain extraction
CN201810215283.XA Expired - Fee Related CN108251288B (en) 2016-04-27 2016-04-27 Anaerobic fecal bacteria extraction device and method
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CN108251288B (en) 2021-10-08
CN105886382B (en) 2018-04-24
CN108264999B (en) 2022-02-11
CN108441412B (en) 2022-12-06
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CN105886382A (en) 2016-08-24
CN108251288A (en) 2018-07-06
CN108467827A (en) 2018-08-31
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CN108485938B (en) 2021-12-17
CN108485938A (en) 2018-09-04

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