CN112226344B - Anaerobic extraction and encapsulation intestinal flora integrated machine - Google Patents

Anaerobic extraction and encapsulation intestinal flora integrated machine Download PDF

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Publication number
CN112226344B
CN112226344B CN202011099512.XA CN202011099512A CN112226344B CN 112226344 B CN112226344 B CN 112226344B CN 202011099512 A CN202011099512 A CN 202011099512A CN 112226344 B CN112226344 B CN 112226344B
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intestinal flora
shell
elution
extraction
anaerobic
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CN112226344A (en
Inventor
刘经未
邱泳明
朱悦
王倩倩
张庆芳
于爽
迟雪梅
刘响
谭晶
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Dalian University
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Dalian University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
    • B65B31/00—Packaging articles or materials under special atmospheric or gaseous conditions; Adding propellants to aerosol containers
    • B65B31/02—Filling, closing, or filling and closing, containers or wrappers in chambers maintained under vacuum or superatmospheric pressure or containing a special atmosphere, e.g. of inert gas
    • B65B31/024—Filling, closing, or filling and closing, containers or wrappers in chambers maintained under vacuum or superatmospheric pressure or containing a special atmosphere, e.g. of inert gas specially adapted for wrappers or bags
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65B—MACHINES, APPARATUS OR DEVICES FOR, OR METHODS OF, PACKAGING ARTICLES OR MATERIALS; UNPACKING
    • B65B51/00—Devices for, or methods of, sealing or securing package folds or closures; Devices for gathering or twisting wrappers, or necks of bags
    • B65B51/10—Applying or generating heat or pressure or combinations thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C12M29/22—Oxygen discharge

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Mechanical Engineering (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Extraction Or Liquid Replacement (AREA)

Abstract

The invention relates to the technical field of intestinal flora separation and extraction devices, in particular to an anaerobic extraction and intestinal flora encapsulation integrated machine. The device comprises a shell, wherein a material inlet is arranged above the shell, a nitrogen gas inlet and an air outlet are also arranged on the shell, two tearing rollers which are arranged oppositely are arranged inside the shell, a scraping roller is arranged below each tearing roller, an elution turntable is arranged below each scraping roller, a filter screen is arranged below the elution turntable, a discharging channel is connected below the shell, and a switch valve is arranged at the outlet of the discharging channel; the outer walls of the tearing rollers are provided with sharp teeth, and the sharp teeth on the two tearing rollers are of a pair-edge structure; the outer wall of the scraping roller is provided with scraping teeth, and the pair of scraping teeth and sharp teeth are of a pair-edge structure; the elution turntable is provided with columnar comb teeth, and the elution turntable is provided with a discharge port. The device ensures anaerobic operation of the intestinal flora in the extraction process, and finally seals the intestinal flora in the sealing bag, thereby avoiding contact between the intestinal flora and air and improving the survival rate of the intestinal flora.

Description

Anaerobic extraction and encapsulation intestinal flora integrated machine
Technical Field
The invention relates to the technical field of intestinal flora separation and extraction devices, in particular to an anaerobic extraction and intestinal flora encapsulation integrated machine.
Background
Extraction of intestinal bacteria from animals requires on-site treatment because of the requirement for an anaerobic environment, otherwise death of anaerobic bacteria is easily caused, thereby further causing RNA degradation. The existing method for extracting the intestinal bacteria of the animals generally adopts direct on-site alcohol surface simple disinfection, and uses a knife to scratch the intestinal tract and directly scrape and wash the inner wall of the intestinal tract; for small intestinal tracts, the scraping and washing are difficult, and the whole intestinal tract is used as an experimental sample for carrying out related experiments of intestinal flora. The method is exposed in the air, so that most of bacteria living in anaerobic environment in the intestinal tract die, nucleic acid degradation is initiated, and the diversity of flora is greatly reduced; for the method taking the whole intestinal flora as research, nucleic acid of small intestine host cells is introduced, and a large error is caused for subsequent flora research.
In patent CN 105886382B, an intestinal content flora extraction device is provided, and negative pressure condition is adopted for extraction, but this method is not suitable for small animal intestinal flora extraction, and the feces cannot be effectively taken out without oxygen in a short time and transferred into the extraction device, so that the intestinal tract must be crushed and extracted. Therefore, research and improvement on the intestinal flora extraction device are still needed.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an anaerobic extraction and encapsulation intestinal flora integrated machine, which is used for improving the inconvenience of the anaerobic extraction and encapsulation intestinal flora in the related art in extracting work sites and time.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides an anaerobic draws and encapsulation intestinal flora all-in-one, includes the casing, is equipped with the material entry above the casing, still is equipped with nitrogen gas access port and air discharge port on the casing, and the casing is inside to be equipped with two tearing gyro wheels of relative arrangement, and each tearing gyro wheel below is equipped with one and scrapes the washing gyro wheel, scrapes the washing gyro wheel below and is equipped with elution carousel, is equipped with the filter screen below the elution carousel, is connected with the discharge channel below the casing, and the exit of discharge channel is equipped with the ooff valve; the outer walls of the tearing rollers are provided with sharp teeth, and the sharp teeth on the two tearing rollers are of a counter-blade structure; the outer wall of the scraping and washing roller is provided with scraping teeth, and the pair of scraping teeth and sharp teeth are of a pair-edge structure; the elution carousel is last to have columnar broach, has the discharge port on the elution carousel.
In the above technical scheme, further, a first driving motor is connected below the elution turntable; the filter screen is arranged between the elution turntable and the driving motor.
In the above technical scheme, further, a hole is formed in the middle of the filter screen for placing the electrode frame to fix the motor.
In the above technical solution, further, the mesh of the filter screen is regular hexagon.
In the above technical scheme, further, the casing below is equipped with the support frame, is fixed with the heat-seal heating plate on the support frame.
In the above technical scheme, further, a circle of annular protrusions are arranged on the lower edge of the channel below the valve and used for fixing the sealing bag.
In the above technical scheme, further, the casing is divided into an upper casing and a lower casing, the upper casing and the lower casing are connected by a rotating shaft hinge, and the upper casing and the lower casing are closed by a closed buckle.
In the above technical scheme, further, sealing rings are arranged at the closing positions of the upper shell and the lower shell.
The invention has the beneficial effects that: the device ensures anaerobic operation of the intestinal flora in the extraction process, and finally is packaged in the sealing bag, thereby avoiding the chance of the intestinal flora contacting with air, improving the survival rate of the intestinal flora and maximally guaranteeing the structural abundance and diversity of the intestinal flora.
Drawings
FIG. 1 is a cross-sectional view of an all-in-one machine of the present invention;
FIG. 2 is a left side view of the housing of the all-in-one machine of the present invention;
FIG. 3 is a front cross-sectional view of the integrated machine of the present invention;
FIG. 4 is a cross-sectional view of the tearing drum;
FIG. 5 is a cross-sectional view of the construction of the combination of the scraping and tearing drum;
FIG. 6 is a top view of the structure of the filter screen;
FIG. 7 is a front view of a sealed bag construction;
fig. 8 is a structural view of the filter screen support frame;
FIG. 9 is a structural view of an elution carousel;
FIG. 10 is a schematic view of the external structure of the present invention;
in the figure: 1. a material inlet; 2. a housing; 3. a nitrogen gas inlet; 4. an air outlet; 5. tearing the roller; 6. scraping and washing the roller; 7. a seal ring; 8. comb teeth; 9. an elution carousel; 10. a filter screen; 11. a filter screen support frame; 12. a first driving motor; 13. opening and closing a valve; 14. an annular protrusion; 15. heat-sealing the heating plate; 16. tines; 17. a discharge port; 18. scraping teeth; 19. a discharge channel; 20. a support frame; 21. a motor frame; 22. a second driving motor; 23. a closing button; 24. a grid of the filter screen; 25. a spindle hinge; 26. support bars of the filter screen support frame.
Detailed Description
The invention is further illustrated below in connection with specific examples, but is not limited in any way.
The anaerobic extraction and encapsulation intestinal flora integrated machine comprises a shell 2, wherein a material inlet 1 is arranged above the shell, a sealing cover is arranged at the material inlet, a nitrogen gas inlet 3 and an air outlet 4 are arranged on the shell, two tearing rollers 5 which are arranged oppositely are arranged inside the shell, a second driving motor 22 on the outer wall of the shell is connected with the tearing rollers through a roller central shaft to provide power for the tearing rollers, sharp teeth 16 are arranged on the outer wall of the tearing rollers, the sharp teeth on the two tearing rollers are of a pair-edge structure, and the two tearing rollers rotate at different speeds to pierce through the tearing intestinal tract, so that the intestinal contents are exposed outside the intestinal tract; a scraping roller 6 is arranged below each tearing roller, a second driving motor 22 is connected with a central shaft of the scraping roller through a gear to provide scraping power for the scraping roller, scraping teeth 18 are arranged on the outer wall of the scraping roller, a pair of scraping teeth and sharp teeth are arranged on the outer wall of the scraping roller, and the scraping teeth scrape the torn intestinal tracts; an elution rotary table 9 is arranged below the scraping and washing roller, columnar comb teeth 8 are arranged on the elution rotary table, the elution rotary table rotates, the comb teeth separate intestinal contents from intestinal walls, and the elution rotary table drives the torn intestinal tracts to rotate and elute; the elution turntable is provided with a discharge port 17 for discharging the extract, a first driving motor 12 is connected below the elution turntable, a filter screen 10 is arranged between the elution turntable and the first driving motor, a hole is arranged in the middle of the filter screen for placing a motor frame 21 to fix the motor, and a filter screen supporting frame 11 is arranged below the filter screen for further fixing the motor and the filter screen; the filter screen is used for separating intestinal canal walls and content solution, the mesh 24 of the filter screen is regular hexagon with the side length of 1mm, and the intestinal canal walls are blocked by the filter screen and stay on the filter screen; a discharge channel 19 is connected below the shell, intestinal canal extract discharged through the elution turntable is discharged through the discharge channel after being filtered by a filter screen, and a switch valve 13 is arranged at the outlet of the discharge channel; the lower edge of the channel below the valve is provided with a circle of annular bulge 14 for fixing a sealing bag, the sealing bag is a plastic bag which can be melted by heat, the opening of the plastic bag is provided with a circle of elastic rubber band which can be contracted, the plastic bag is sleeved at the annular bulge, and the plastic bag is fixed; a pressing type heat sealing heating plate 15 is fixed on a supporting frame 20 below the shell; and after the extract flows into the sealing bag through the valve, the sealing bag is sealed by heat sealing.
The housing is divided into an upper housing and a lower housing, which are connected by a hinge 25, and which are closed by a closing button 23. The closing position of the upper shell and the lower shell is provided with a sealing ring 7. The diameter of the sealing ring is slightly smaller than the inward sinking of the column body of the shell, the diameter of the sealing ring is the same as the inward sinking diameter of the upper shell and the lower shell, and the sealing ring is better isolated from the external air in the closed state. The upper shell and the lower shell are opened, and the elution turntable and the filter screen are detachable, so that the cleaning and disinfection work inside the machine is facilitated.
When the device is used, intestinal eluent and the intestinal tract are added from the material adding port in sequence, the liquid level of the intestinal eluent is over the scraping teeth below the scraping and washing roller so as to achieve the effect of scraping and washing the intestinal tract, the material inlet is tightly covered, constant-pressure nitrogen is introduced from one end of the nitrogen, under the interaction of gases for a period of time, air is discharged from the air outlet, the inside of the device is ensured to be free of oxygen, all driving motors are started, the intestinal tract is torn by the sharp teeth under the differential rotation of the tearing roller and the intestinal tract content in the intestinal tract is exposed, and simultaneously the torn intestinal tract is transferred to the lower part to be contacted with the scraping and washing roller under the rotation of the tearing roller.
Many possible variations and modifications of the disclosed technology can be made by anyone skilled in the art without departing from the scope of the technology, or the technology can be modified to be equivalent. Therefore, any simple modification, equivalent variation and modification of the above embodiments according to the technical substance of the present invention shall still fall within the scope of the technical solution of the present invention.

Claims (8)

1. The anaerobic extraction and encapsulation intestinal flora integrated machine is characterized by comprising a shell, wherein a material inlet is arranged above the shell, a nitrogen gas inlet and an air outlet are also arranged on the shell, two tearing rollers which are arranged oppositely are arranged inside the shell, a scraping roller is arranged below each tearing roller, an elution turntable is arranged below each scraping roller, a filter screen is arranged below each elution turntable, a discharging channel is connected below the shell, and a switch valve is arranged at the outlet of each discharging channel; the outer walls of the tearing rollers are provided with sharp teeth, and the sharp teeth on the two tearing rollers are of a counter-blade structure; the outer wall of the scraping and washing roller is provided with scraping teeth, and the pair of scraping teeth and sharp teeth are of a pair-edge structure; the elution carousel is last to have columnar broach, has the discharge port on the elution carousel.
2. The anaerobic extraction and encapsulation intestinal flora integrated machine according to claim 1, wherein a first driving motor is connected below the elution turntable; the filter screen is arranged between the elution turntable and the driving motor.
3. The anaerobic extraction and encapsulation intestinal flora integrated machine according to claim 1, wherein a hole is arranged in the middle of the filter screen for placing an electrode frame fixing motor.
4. The anaerobic extraction and encapsulation intestinal flora integrated machine according to claim 1, wherein the mesh of the filter screen is regular hexagon.
5. The anaerobic extraction and encapsulation intestinal flora integrated machine according to claim 1, wherein a supporting frame is arranged below the shell, and a heat-sealing heating plate is fixed on the supporting frame.
6. The anaerobic integrated intestinal flora extraction and packaging machine according to claim 1, wherein the lower edge of the channel below the valve is provided with a ring-shaped bulge for fixing the sealing bag.
7. The anaerobic integrated intestinal flora extraction and packaging machine according to claim 1, wherein the housing is divided into an upper housing and a lower housing, the upper housing and the lower housing are hinged by a rotating shaft, and the upper housing and the lower housing are closed by a closing buckle.
8. The anaerobic integrated intestinal flora extraction and packaging machine according to claim 7, wherein sealing rings are arranged at the closing positions of the upper shell and the lower shell.
CN202011099512.XA 2020-10-14 2020-10-14 Anaerobic extraction and encapsulation intestinal flora integrated machine Active CN112226344B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202011099512.XA CN112226344B (en) 2020-10-14 2020-10-14 Anaerobic extraction and encapsulation intestinal flora integrated machine

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Application Number Priority Date Filing Date Title
CN202011099512.XA CN112226344B (en) 2020-10-14 2020-10-14 Anaerobic extraction and encapsulation intestinal flora integrated machine

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CN112226344A CN112226344A (en) 2021-01-15
CN112226344B true CN112226344B (en) 2023-08-18

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105886382A (en) * 2016-04-27 2016-08-24 烟台民望机电技术有限公司 Intestinal content flora extraction device

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3035317B1 (en) * 2015-04-24 2019-06-14 Maat Pharma MICROORGANISM SAMPLING DEVICE, SAMPLING KIT COMPRISING SUCH A DEVICE AND SAMPLING METHOD USING SUCH A DEVICE

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105886382A (en) * 2016-04-27 2016-08-24 烟台民望机电技术有限公司 Intestinal content flora extraction device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Current sampling methods for gut microbiota:a call for more precise devices;Qiang tang et al;Frontiers in cellular and infection microbiology(第10期);1-10 *

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