CN105886382A - Intestinal content flora extraction device - Google Patents
Intestinal content flora extraction device Download PDFInfo
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- CN105886382A CN105886382A CN201610269238.3A CN201610269238A CN105886382A CN 105886382 A CN105886382 A CN 105886382A CN 201610269238 A CN201610269238 A CN 201610269238A CN 105886382 A CN105886382 A CN 105886382A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
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Abstract
The invention discloses an intestinal content flora extraction device which comprises a crushing device, a filtering device and a negative pressure device. The crushing device comprises a crushing bucket with a sealing cover, an annular groove is formed in each of the upper end face of the crushing bucket and the end face of a port of the sealing cover, annular blades in matched use are arranged in the two annular grooves, a filtering net is arranged on the inner wall of the bottom of the crushing bucket, spiny protrusions are uniformly distributed on the upper end face of the filtering net, a rotating shaft completely provided with spiny protrusions is arranged on the side wall of the crushing bucket positioned above the filtering net, a round groove with an air hole is formed in the sealing cover, an air valve cover hinged to the sealing cover is arranged in the round groove, the crushing bucket is filled with a diluent, and a liquid outlet with a switch valve is arranged on the side wall of the lower portion of the crushing bucket. The intestinal content flora extraction device has the advantages that the intestinal content flora extraction device is convenient to operate, a plastic sealing bag is combined to collect excrement samples, time of contact between the excrement samples and air is reduced as much as possible, and Coprophilous fungi survival rate and treatment effect are improved.
Description
Technical field
The present invention relates to a kind of medical extraction element, particularly relate to a kind of intestinal contents flora extraction element.
Background technology
It is a kind of by rebuilding the intestinal microbial population method for the treatment of disease that feces is transplanted, and the feces of Healthy People will obtain useful caprophyl liquid after treatment, i.e. remove the flora that the parasites such as worm's ovum only remain with, be then fabricated to enteric coated capsule or between oral.Medical worker when preparing stool suspension either from vision, olfactory sensation or psychologically the most beyond affordability, and want the activity ensureing caprophyl must be rapidly completed extraction process, owing to the flora overwhelming majority in intestinal is anaerobic bacterium, when medical worker takes stool sample, the time of stool sample one section of ingress of air, being difficult to ensure the most very much the condition of card anaerobic during extracting, the most current extraction equipment is it is difficult to ensure that the quality of caprophyl.
Summary of the invention
The invention provides a kind of feces of minimizing as far as possible and the time of contact of air, the easy to operate and intestinal contents flora extraction element of raising caprophyl survival rate.
The present invention is realized by following measure: a kind of intestinal contents flora extraction element, the defecator being connected with described breaker including breaker and the negative pressure device being connected with described defecator；
Described breaker include with seal lid broken bucket, the upper surface of described broken bucket and the end face of described sealing lid port are provided with annular groove, be provided with in two described annular grooves with the use of circular blade；The medial wall height of described sealing bezel ring, connected in star is less than the lateral wall of described sealing bezel ring, connected in star；
It is provided with a drainage screen on described broken bottom of the barrel inwall, described drainage screen upper surface is evenly equipped with spine projection, being positioned at above described drainage screen and be provided with the rotating shaft being covered with spine projection on described broken bucket sidewall, described rotating shaft one end passes described broken bucket sidewall and is provided with handle；
Described sealing covers and is provided with the leachy circular groove of band, is provided with and seals, with described, the air valve cap that lid is hinged in described circular groove；
Described diluent is filled with in described broken bucket；It is provided with the liquid outlet with switch valve on described broken bucket lower sides.
Described defecator includes the filtration container sealed, it is provided with inlet in the upper portion side wall of described filtration container, described inlet is connected with the liquid outlet of described breaker, it is provided with liquid outlet on the lower sides of described filtration container, it is positioned at above described liquid outlet and is provided with negative pressure ventilation mouth on described filtration container side wall, the filter membrane that some levels aperture from top to bottom reduces step by step it is provided with in described filtration container between described inlet and described negative pressure ventilation mouth, interval is had between filter membrane described in adjacent two-stage, it is provided with on the sidewall of described filtration container between filter membrane described in adjacent two-stage with the leakage fluid dram sealing lid；
The upper surface of described filtration container is provided with the leachy circular groove of band, is provided with the air valve cap hinged with described filtration container upper end face in described circular groove.
The height of described circular blade is less than lateral wall height and the medial wall height of described annular groove of described annular groove.
The sealing lid of described breaker is threadeded with described broken bucket, and described sealing lid is provided with female thread, and described broken bucket is provided with the external screw thread being connected with described female thread.
Be positioned at outside described annular groove and on the sidewall of described broken bucket, be evenly arranged with several L-type breach, be positioned at outside described annular groove described seal lid wall outer surface be provided with described L-type breach with the use of locating dowel.
Preferably, the invention provides a kind of intestinal contents flora extraction element, the extraction tube being connected including breaker and the negative pressure device being connected with described extraction tube with breaker；Described breaker include with seal lid broken bucket, the upper surface of described broken bucket and the end face of described sealing lid port are provided with annular groove, be provided with in two described annular grooves with the use of circular blade；The medial wall height of described sealing bezel ring, connected in star is less than the lateral wall of described sealing bezel ring, connected in star；
It is provided with a drainage screen on described broken bottom of the barrel inwall, described drainage screen upper surface is evenly equipped with spine projection, being positioned at above described drainage screen and be provided with the rotating shaft being covered with spine projection on described broken bucket sidewall, described rotating shaft one end passes described broken bucket sidewall and is provided with handle；
Described sealing covers and is provided with the leachy circular groove of band, is provided with and seals, with described, the air valve cap that lid is hinged in described circular groove；
Described diluent is filled with in described broken bucket；It is provided with the liquid outlet with switch valve on described broken bucket lower sides.
The height of described circular blade is less than the lateral wall height of described annular groove.
The described lid that seals is threadeded with described broken bucket, and described sealing lid is provided with female thread, and described broken bucket is provided with the external screw thread being connected with described female thread；Or, be positioned at outside described annular groove and be provided with L-type breach on the sidewall of described broken bucket, be positioned at outside described annular groove described seal lid wall outer surface be provided with described L-type breach with the use of locating dowel.
Described extraction tube includes the filter membrane that body and some levels are connected with the inner wall sealing of described body, described body upper end is provided with inlet, described body lower end is provided with the negative pressure ventilation mouth being connected with negative pressure device, sealing on the sidewall of body described in described body lower end and be provided with a hard liquid storage box connected with described body, described filter membrane aperture from top to bottom is in the trend reduced step by step.
The material of described body is silica gel, soft rubber, PVC or flexible plastic, and one-body molded；The aperture of described filter membrane is 0.0004-5.00mm.
Preferably, present invention also offers a kind of intestinal contents flora extraction element, described extraction element includes sample collecting device and collecting bag；Described sample collecting device includes the cylindrical collection container of upper end open and is arranged on the upper cover of described collection container upper surface, cover on described and be provided with manhole or ellipse hole, the lower section of described upper cover is provided with inverted cone collecting hood, and described collecting hood lower port arranges described collecting bag；
Described collecting bag is provided with black box on the sidewall of opening, and described black box can be enclosed within the lower port of described collecting hood；
Diluent is held in described collection container.
Described black box is magnetic stripe, and described collecting bag is symmetrically arranged with two described magnetic stripes on the sidewall of opening, and the two ends of two described magnetic stripes are articulated and connected.
The upper surface of described collection container bend inwards formation annular stop plate, described annular stop plate upper surface is provided with annular groove, described upper cover lower surface be provided with described annular groove with the use of annular protrusion.
Be provided with on the sidewall of described collection container and take thing mouth, the sidewall of described collection container is hinged with described take thing mouth corresponding take thing door.
Magnetic stripe one end of described collecting bag connects stay cord.
Preferably, invention further provides a kind of intestinal contents flora extraction element, described extraction element includes sample collecting device and collecting bag；Described sample collecting device includes the cylindrical collection container of upper end open and is arranged on the upper cover of described collection container upper surface, cover on described and be provided with manhole or ellipse hole, the lower section of described upper cover is provided with inverted cone collecting hood, and described collecting hood lower port arranges described collecting bag；
Described collecting bag is provided with black box on the sidewall of opening, and described black box can be enclosed within the lower port of described collecting hood；
Diluent is held in described collection container.
Described black box is magnetic stripe, and described collecting bag is symmetrically arranged with two described magnetic stripes on the sidewall of opening, and the two ends of two described magnetic stripes are articulated and connected.
The upper surface of described collection container bend inwards formation annular stop plate, described annular stop plate upper surface is provided with annular groove, described upper cover lower surface be provided with described annular groove with the use of annular protrusion.
Be provided with on the sidewall of described collection container and take thing mouth, the sidewall of described collection container is hinged with described take thing mouth corresponding take thing door.
Magnetic stripe one end of described collecting bag connects stay cord.
Described extraction element also includes breaker, described breaker includes with the broken bucket sealing lid, be provided with annular groove on the upper surface of described broken bucket and the end face of described sealing lid port, be provided with in two described annular grooves with the use of circular blade；The medial wall height of described sealing bezel ring, connected in star is less than the lateral wall of described sealing bezel ring, connected in star；
It is provided with a drainage screen on described broken bottom of the barrel inwall, described drainage screen upper surface is evenly equipped with spine projection, being positioned at above described drainage screen and be provided with the rotating shaft being covered with spine projection on described broken bucket sidewall, described rotating shaft one end passes described broken bucket sidewall and is provided with handle；
Described sealing covers and is provided with the leachy circular groove of band, is provided with and seals, with described, the air valve cap that lid is hinged in described circular groove；
Described diluent is filled with in described broken bucket；It is provided with the liquid outlet with switch valve on described broken bucket lower sides.
The height of described circular blade is less than the lateral wall height of described annular groove.
The described lid that seals is threadeded with described broken bucket, and described sealing lid is provided with female thread, and described broken bucket is provided with the external screw thread being connected with described female thread；Or, be positioned at outside described annular groove and be provided with L-type breach on the sidewall of described broken bucket, be positioned at outside described annular groove described seal lid wall outer surface be provided with described L-type breach with the use of locating dowel.
Described extraction element also includes that defecator, described defecator include feed liquor cylinder, several intermediate cylinders and go out fluid cylinder, is provided with cambered surface drainage screen in described intermediate cylinder and described discharge barrel；Described intermediate cylinder near the medial wall of upper port and described go out fluid cylinder on the inwall of upper port all on be provided with annular gear edge, described cambered surface drainage screen includes annular backup pad and is arranged on the cambered surface supporting network of described annular backup pad lower surface, being provided with filter membrane in the mesh of described cambered surface supporting network, described annular backup pad is carried on a shoulder pole on described annular backup pad；
Described intermediate cylinder upper port is provided with female thread, described intermediate cylinder lower port is provided with external screw thread, the external screw thread that described feed liquor cylinder lower port is provided with and described intermediate cylinder screw-internal thread fit uses, described in go out the female thread that the upper port of fluid cylinder is provided with and described intermediate cylinder screw-internal thread fit uses；Several described intermediate cylinders are threaded connection；
Described go out fluid cylinder lower end be provided with liquid outlet, described in go out and be provided with negative pressure ventilation mouth on the sidewall of fluid cylinder；
It is provided with the leachy circular groove of band on described feed liquor cylinder upper surface, is provided with in described circular groove and seals, with described, the air valve cap that lid is hinged.
Several flexible cleaning balls are placed in described cambered surface drainage screen.
The invention has the beneficial effects as follows: the present invention is the most easy to operate, and combine plastic sealing bag and gather fecal sample, less fecal sample and the time of contact of air, improve caprophyl survival rate and therapeutic effect；nullDuring use，The fecal sample of Healthy People is put in plastic sealing bag by the very first time，Feces is kept to be in oxygen-free environment，Then it is placed on sealing bag on the upper surface of the broken bucket filling with diluent，Then lid top cover labyrinth，Sealing lid moves downward，Can cut in half sealing bag when the circular blade of broken bucket and the circular blade of sealing lid contact，Half containing fecal sample automatically falls in broken bucket，Crank handle drives axis of rotation，Spine projection in rotating shaft and the spine projection generation relative movement on drainage screen，Seal bag to be scratched rapidly，Feces is through dilution and passes through filter screen filtration，Obtain removing the fecal liquid of large granular impurity，Open the switch valve of liquid outlet，Fecal liquid is discharged in the defecator of negative pressure evacuation，After fecal liquid fully enters defecator，Closing switch valve，Open the air valve cap filtering container upper end face，Fecal liquid finally gives intestinal beneficial flora after multistage filtering.
Accompanying drawing explanation
Fig. 1 is the structural representation of the embodiment of the present invention 1.
Fig. 2 is the structural representation of broken bucket in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 3 is the structural representation that in the embodiment of the present invention 1, embodiment 2 and embodiment 4, breaker seals lid.
Fig. 4 is the another kind of structural representation of broken bucket in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 5 is the structural representation of defecator in the embodiment of the present invention 1.
Fig. 6 is the structural representation of air valve cap in the embodiment of the present invention 1.
Fig. 7 is the structural representation of the embodiment of the present invention 2.
Fig. 8 is the structural representation of defecator in the embodiment of the present invention 2.
Fig. 9 is the structural representation of harvester in the embodiment of the present invention 3 and embodiment 4.
Figure 10 is the structural representation of collecting bag in the embodiment of the present invention 3 and embodiment 4.
Figure 11 is the schematic diagram that in the embodiment of the present invention 3 and embodiment 4, harvester uses state.
Figure 12 is harvester and the structural representation of defecator in the embodiment of the present invention 4.
Figure 13 is the structural representation of defecator in the embodiment of the present invention 4.
In figure, 1, broken bucket；2, drainage screen；3, bearing；4, rotating shaft；5, spine projection；6, lid is sealed；7, air valve cap；8, handle；9, liquid outlet；10, connecting tube；11, inlet；12, container is filtered；13, negative pressure ventilation mouth；14, liquid outlet；15, external screw thread；16, the lateral wall of annular groove；17, circular blade；18, annular groove medial wall；19, L-type breach；20, filter membrane；21, leakage fluid dram；22, pore；23, air valve cap；24, projection is sealed；25, extraction tube；26, hard liquid storage box；27, collection container；28, diluent；29, collecting bag；30, collecting hood；31, annular protrusion；32, annular stop plate；33, upper cover；34, thing mouth is taken；35, stay cord；36, jointed shaft；37, magnetic stripe；38, edge on collecting bag；39, snap close；40, ellipse hole；41, thing door is taken；42, feed liquor cylinder；43, intermediate cylinder；44, fluid cylinder is gone out；45, annular gear edge；46, annular backup pad；47, flexible cleaning ball；48, cambered surface supporting network.
Detailed description of the invention
For the technical characterstic of this programme can be clearly described, below by detailed description of the invention, this programme is illustrated.
Embodiment 1
See Fig. 1-Fig. 6, a kind of intestinal contents flora extraction element, the defecator being connected including breaker and the negative pressure device being connected with defecator with breaker；
Breaker includes with the broken bucket 1 sealing lid 6, it is provided with annular groove on the upper surface of broken bucket 1 and the end face of sealing lid 6 ports, be provided with in two annular grooves with the use of circular blade 17, be provided with in i.e. crushing in bucket 1 upper surface and sealing the port end face of lid 6 with the use of circular blade 17, play the effect of cut-out；Sealing medial wall 18 height lateral wall 16 less than sealing lid 6 annular grooves of lid 6 annular grooves, broken cut-off parts within bucket 1 can be fallen in broken bucket 1 smoothly；The height of circular blade 17 is less than lateral wall 16 height of annular groove and medial wall 18 height of annular groove.
It is provided with a drainage screen 2 in broken bucket 1 bottom interior wall, drainage screen 2 upper surface is evenly equipped with spine projection 5, it is positioned at above drainage screen 2 and is provided with, on broken bucket 1 sidewall, the rotating shaft 4 being covered with spine projection 5, the two ends of rotating shaft 4 are horizontally set on the sidewall of broken bucket 1 by bearing 3, and rotating shaft 4 wherein one end passes broken bucket 1 sidewall and is provided with handle 8；
Seal and be provided with the circular groove with pore 22 on lid 6, be provided with in circular groove and seal in lid 6 hinged air valve cap 7(or figure 23), in air valve cap 7(or figure 23) on be provided with the sealing projection 24 corresponding with pore 22；
Diluent 28 is filled with in broken bucket 1；It is provided with the liquid outlet 14 with switch valve on broken bucket 1 lower sides.
The sealing lid 6 of breaker is threadeded with broken bucket 1, seals lid and is provided with female thread, and broken bucket 1 is provided with the external screw thread 15 being connected with female thread；Or, be positioned at outside annular groove on the sidewall of broken bucket 1 and be evenly arranged with several L-type breach 19, be positioned at the described wall outer surface sealing lid outside annular groove be provided with L-type breach 19 with the use of locating dowel.
Defecator includes the filtration container 12 sealed, filter and be provided with inlet 11 in the upper portion side wall of container 12, inlet 11 is connected with the liquid outlet 9 of breaker by connecting tube 10, filter and be provided with liquid outlet 14 on the lower sides of container 12, it is positioned at above liquid outlet 14 to filter and is provided with negative pressure ventilation mouth 13 on container 12 sidewall, it is provided with, in filtering container 12 between inlet 11 and negative pressure ventilation mouth 13, the filter membrane 20 that some levels aperture from top to bottom reduces step by step, interval is had between adjacent double-filtration film 20, filter between adjacent double-filtration film 20 and be provided with the leakage fluid dram 21 sealing lid on the sidewall of container 12；
The upper surface filtering container 12 is provided with the circular groove with pore 22, is provided with the air valve cap 23 hinged with filtering container 12 upper surface, air valve cap 23 is provided with the sealing projection 24 corresponding with pore 22 in circular groove.Wherein, negative pressure device is prior art, does not repeats them here.
nullDuring use，The fecal sample of Healthy People is put in plastic sealing bag by the very first time，Feces is kept to be in oxygen-free environment，Then it is placed on sealing bag on the upper surface of the broken bucket 1 filling with diluent 28，Then lid top cover labyrinth 6，Seal lid 6 to move downward，Can cut in half sealing bag when the circular blade of broken bucket 1 and the circular blade of sealing lid 6 contact，Half containing fecal sample automatically falls in broken bucket 1，Crank handle 8 drives rotating shaft 4 to rotate，Relative movement is there is in the spine projection 5 in rotating shaft 4 with the spine projection 5 on drainage screen 2，Seal bag to be scratched rapidly，Feces filters through dilution and by drainage screen 2，Obtain removing the fecal liquid of large granular impurity，Open the switch valve of liquid outlet，Fecal liquid is discharged in the defecator of negative pressure evacuation，After fecal liquid fully enters defecator，Closing switch valve，Open the air valve cap 23 filtering container 12 upper surface，Fecal liquid finally gives intestinal beneficial flora after multistage filtering.
Embodiment 2
See Fig. 2, Fig. 3, Fig. 4, Fig. 7 and Fig. 8, the invention provides a kind of intestinal contents flora extraction element, the extraction tube 25 being connected with breaker including breaker and the negative pressure device being connected with extraction tube 25；Breaker includes with the broken bucket 1 sealing lid 6, it is provided with annular groove on the upper surface of broken bucket 1 and the end face of sealing lid 6 ports, be provided with in two annular grooves with the use of circular blade 17, be provided with in i.e. crushing in bucket 1 upper surface and sealing the port end face of lid 6 with the use of circular blade 17, play the effect of cut-out；Sealing medial wall 18 height lateral wall 16 less than sealing lid 6 annular grooves of lid 6 annular grooves, broken cut-off parts within bucket 1 can be fallen in broken bucket 1 smoothly；The height of circular blade 17 is less than lateral wall 16 height of annular groove and medial wall 18 height of annular groove.
It is provided with a drainage screen 2 in broken bucket 1 bottom interior wall, drainage screen 2 upper surface is evenly equipped with spine projection 5, it is positioned at above drainage screen 2 and is provided with, on broken bucket 1 sidewall, the rotating shaft 4 being covered with spine projection 5, the two ends of rotating shaft 4 are horizontally set on the sidewall of broken bucket 1 by bearing 3, and rotating shaft 4 wherein one end passes broken bucket 1 sidewall and is provided with handle 8；
Seal and be provided with the circular groove with pore 22 on lid 6, be provided with in circular groove and seal in lid 6 hinged air valve cap 7(or figure 23), in air valve cap 7(or figure 23) on be provided with the sealing projection 24 corresponding with pore 22；
Diluent 28 is filled with in broken bucket 1；It is provided with the liquid outlet 14 with switch valve on broken bucket 1 lower sides.
The sealing lid 6 of breaker is threadeded with broken bucket 1, seals lid and is provided with female thread, and broken bucket 1 is provided with the external screw thread 15 being connected with female thread；Or, be positioned at outside annular groove on the sidewall of broken bucket 1 and be evenly arranged with several L-type breach 19, be positioned at the described wall outer surface sealing lid outside annular groove be provided with L-type breach 19 with the use of locating dowel.
Extraction tube 25 includes the filter membrane 20 that body and some levels are connected with the inner wall sealing of body, body upper end is provided with inlet 11, the liquid outlet 9 of breaker is connected with inlet 11, body lower end is provided with the negative pressure ventilation mouth 13 being connected with negative pressure device, sealing on the sidewall of body lower end body and be provided with a hard liquid storage box 26 connected with body, filter membrane 20 aperture from top to bottom is in the trend reduced step by step.
The material of body is silica gel, soft rubber, PVC or flexible plastic, and the material of hard liquid storage 26 box is rigid plastics, and the upper port of hard liquid storage box 26 is one-body molded with body；The aperture of filter membrane 20 is 0.0004-5.00mm.
nullDuring use，The fecal sample of Healthy People is put in plastic sealing bag by the very first time，Feces is kept to be in oxygen-free environment，Then it is placed on sealing bag on the upper surface of the broken bucket 1 filling with diluent 28，Then lid top cover labyrinth 6，Seal lid 6 to move downward，Can cut in half sealing bag when the circular blade of broken bucket 1 and the circular blade of sealing lid 6 contact，Half containing fecal sample automatically falls in broken bucket 1，Crank handle 8 drives rotating shaft 4 to rotate，Relative movement is there is in the spine projection 5 in rotating shaft 4 with the spine projection 5 on drainage screen 2，Seal bag to be scratched rapidly，Feces filters through dilution and by drainage screen 2，Obtain removing the fecal liquid of large granular impurity，Open the switch valve of liquid outlet，Fecal liquid is discharged in the defecator of negative pressure evacuation，After fecal liquid fully enters defecator，Closing switch valve，Fecal liquid finally gives intestinal beneficial flora after multistage filtering.
Embodiment 3
Seeing Fig. 9, Figure 10 and Figure 11, embodiments provide a kind of intestinal contents flora extraction element, extraction element includes sample collecting device and collecting bag 29；Sample collecting device includes the cylindrical collection container 27 of upper end open and is arranged on the upper cover 33 of collection container 27 upper surface, manhole or ellipse hole 40 it is provided with on upper cover 33, the lower section of upper cover 33 is provided with inverted cone collecting hood 30, and collecting hood 30 lower port arranges collecting bag 29；
Collecting bag 29 is provided with black box on the sidewall of opening (along 38 on collecting bag), and black box can be enclosed within the lower port of collecting hood 30；Diluent 28 is held in collection container.
Black box is magnetic stripe 37, and collecting bag 29 is symmetrically arranged with two magnetic stripes 37 on the sidewall of opening, and the two ends of two magnetic stripes 37 are articulated and connected by jointed shaft 36.
The upper surface of collection container 27 bend inwards formation annular stop plate 32, annular stop plate 32 upper surface is provided with annular groove, upper cover 33 lower surface be provided with annular groove with the use of annular protrusion 31.
It is provided with on the sidewall of collection container 27 and takes thing mouth 34, the sidewall of collection container 27 is hinged with and corresponding with taking thing mouth 34 takes thing door 41.It is provided with graduation mark on the inwall of collection container 27, the liquid level just contact sleeve of guarantee diluent 28 collecting bag 29 on collecting hood 30, magnetic stripe 37 enables to collecting bag and is just enclosed within collecting hood 39 and does not falls, when there being feces to enter collecting bag, gravity can cause collecting bag 39 to move downward.
Magnetic stripe 37 one end of collecting bag 29 is connected by snap close 39 stay cord 35.
During use, diluent 28 to graduation mark is held by collection container 27, upper cover 33 side of collecting bag 29 will be packaged on collection container 27, the healthy personnel of sample to be collected are sitting on upper cover 33, when there being feces to enter collecting bag, collecting bag can cause collecting bag 39 to move downward due to gravity increase, when departing from collecting hood 30, magnetic stripe 37 attracts rapidly and gets rid of the gas in collecting bag under the pressure of diluent as far as possible, improves the anaerobic quality in collecting bag 39.
Embodiment 4
Seeing Fig. 2-Fig. 4 and Fig. 9-Figure 13, embodiments provide a kind of intestinal contents flora extraction element, described extraction element includes sample collecting device and collecting bag 29；Sample collecting device includes the cylindrical collection container 27 of upper end open and is arranged on the upper cover 33 of collection container 27 upper surface, manhole or ellipse hole 40 it is provided with on upper cover 33, the lower section of upper cover 33 is provided with inverted cone collecting hood 30, and collecting hood 30 lower port arranges collecting bag 29；
Collecting bag 29 is provided with black box on the sidewall of opening, and black box can be enclosed within the lower port of collecting hood 30；Diluent 28 is held in collection container.
Black box is magnetic stripe 37, and collecting bag 29 is symmetrically arranged with two magnetic stripes 37 on the sidewall of opening, and the two ends of two magnetic stripes 37 are articulated and connected.
The upper surface of collection container 27 bend inwards formation annular stop plate 32, annular stop plate 32 upper surface is provided with annular groove, upper cover 33 lower surface be provided with annular groove with the use of annular protrusion 31.
It is provided with on the sidewall of collection container 27 and takes thing mouth 34, the sidewall of collection container 27 is hinged with and corresponding with taking thing mouth 34 takes thing door 41.
Magnetic stripe 37 one end of collecting bag 29 is connected by snap close 39 stay cord 35.
Described extraction element also includes breaker, breaker includes with the broken bucket 1 sealing lid 6, it is provided with annular groove on the upper surface of broken bucket 1 and the end face of sealing lid 6 ports, be provided with in two annular grooves with the use of circular blade 17, be provided with in i.e. crushing in bucket 1 upper surface and sealing the port end face of lid 6 with the use of circular blade 17, play the effect cutting off collecting bag 29 seal；Sealing medial wall 18 height lateral wall 16 less than sealing lid 6 annular grooves of lid 6 annular grooves, broken cut-out collecting bag within bucket 1 can be fallen in broken bucket 1 smoothly；The height of circular blade 17 is less than lateral wall 16 height of annular groove and medial wall 18 height of annular groove.
It is provided with a drainage screen 2 in broken bucket 1 bottom interior wall, drainage screen 2 upper surface is evenly equipped with spine projection 5, it is positioned at above drainage screen 2 and is provided with, on broken bucket 1 sidewall, the rotating shaft 4 being covered with spine projection 5, the two ends of rotating shaft 4 are horizontally set on the sidewall of broken bucket 1 by bearing 3, and rotating shaft 4 wherein one end passes broken bucket 1 sidewall and is provided with handle 8；
Seal and be provided with the circular groove with pore 22 on lid 6, be provided with in circular groove and seal in lid 6 hinged air valve cap 7(or figure 23), in air valve cap 7(or figure 23) on be provided with the sealing projection 24 corresponding with pore 22；
Diluent 28 is filled with in broken bucket 1；It is provided with the liquid outlet 14 with switch valve on broken bucket 1 lower sides.
The sealing lid 6 of breaker is threadeded with broken bucket 1, seals lid and is provided with female thread, and broken bucket 1 is provided with the external screw thread 15 being connected with female thread；Or, be positioned at outside annular groove on the sidewall of broken bucket 1 and be evenly arranged with several L-type breach 19, be positioned at the described wall outer surface sealing lid outside annular groove be provided with L-type breach 19 with the use of locating dowel.
Described extraction element also includes that defecator, described defecator include feed liquor cylinder 42, several intermediate cylinders 43 and go out fluid cylinder 44, is provided with cambered surface drainage screen in intermediate cylinder 43 and discharge barrel 44；Intermediate cylinder 43 near the medial wall of upper port and go out fluid cylinder 44 on the inwall of upper port all on be provided with annular gear along 45, cambered surface drainage screen includes annular backup pad 46 and is arranged on the cambered surface supporting network 48 of annular backup pad 46 lower surface, be provided with filter membrane in the mesh of cambered surface supporting network 48, annular backup pad 46 carry on a shoulder pole annular gear along 45 and by above adjacent intermediate cylinder 43 lower port be pressed on annular gear along 45；
Intermediate cylinder 43 upper port is provided with female thread, intermediate cylinder 43 lower port is provided with external screw thread, feed liquor cylinder 42 lower port is provided with the external screw thread used with intermediate cylinder 43 screw-internal thread fit, and the upper port going out fluid cylinder 44 is provided with the external screw thread used with intermediate cylinder 43 screw-internal thread fit；Several intermediate cylinders 43 are threaded connection；
Go out fluid cylinder 44 lower end and be provided with liquid outlet 14, go out and on the sidewall of fluid cylinder 44, be provided with negative pressure ventilation mouth 13；
It is provided with the leachy circular groove of band on feed liquor cylinder 42 upper surface, in circular groove, is provided with and seals the air valve cap that lid is hinged；Or feed liquor cylinder is soft material, such as silica gel, soft rubber, PVC or flexible plastic with the sidewall going out fluid cylinder and intermediate cylinder.Placing several flexible cleaning balls 47 in cambered surface drainage screen, flexible cleaning ball 47 is the spheroid that soft material matter and surface are covered with thorn, and flexible cleaning ball 47 is prior art, does not repeats them here.
During use, diluent 28 to graduation mark is held by collection container 27, upper cover 33 side of collecting bag 29 will be packaged on collection container 27, the healthy personnel of sample to be collected are sitting on upper cover 33, when there being feces to enter collecting bag, collecting bag can cause collecting bag 39 to move downward due to gravity increase, when departing from collecting hood 30, magnetic stripe 37 attracts rapidly and gets rid of the gas in collecting bag under the pressure of diluent as far as possible, improves the anaerobic quality in collecting bag 39.Then, it is placed on sealing bag on the upper surface of the broken bucket 1 filling with diluent 28, then lid top cover labyrinth 6, seal lid 6 to move downward, can cut in half sealing bag when the circular blade of broken bucket 1 and the circular blade of sealing lid 6 contact, half containing fecal sample automatically falls in broken bucket 1, crank handle 8 drives rotating shaft 4 to rotate, relative movement is there is in the spine projection 5 in rotating shaft 4 with the spine projection 5 on drainage screen 2, seal bag to be scratched rapidly, feces filters through dilution and by drainage screen 2, obtain removing the fecal liquid of large granular impurity, open the switch valve of liquid outlet, fecal liquid is discharged in the defecator of negative pressure evacuation, after fecal liquid fully enters defecator, closing switch valve, fecal liquid finally gives intestinal beneficial flora after multistage filtering.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (5)
1. an intestinal contents flora extraction element, the defecator being connected with described breaker including breaker and the negative pressure device being connected with described defecator；
Described breaker include with seal lid broken bucket, the upper surface of described broken bucket and the end face of described sealing lid port are provided with annular groove, be provided with in two described annular grooves with the use of circular blade；The medial wall height of described sealing bezel ring, connected in star is less than the lateral wall of described sealing bezel ring, connected in star；
It is provided with a drainage screen on described broken bottom of the barrel inwall, described drainage screen upper surface is evenly equipped with spine projection, being positioned at above described drainage screen and be provided with the rotating shaft being covered with spine projection on described broken bucket sidewall, described rotating shaft one end passes described broken bucket sidewall and is provided with handle；
Described sealing covers and is provided with the leachy circular groove of band, is provided with and seals, with described, the air valve cap that lid is hinged in described circular groove；
Diluent is filled with in described broken bucket；It is provided with the liquid outlet with switch valve on described broken bucket lower sides.
Intestinal contents flora extraction element the most according to claim 1, it is characterized in that, described defecator includes the filtration container sealed, it is provided with inlet in the upper portion side wall of described filtration container, described inlet is connected with the liquid outlet of described breaker, it is provided with liquid outlet on the lower sides of described filtration container, it is positioned at above described liquid outlet and is provided with negative pressure ventilation mouth on described filtration container side wall, the filter membrane that some levels aperture from top to bottom reduces step by step it is provided with in described filtration container between described inlet and described negative pressure ventilation mouth, interval is had between filter membrane described in adjacent two-stage, it is provided with on the sidewall of described filtration container between filter membrane described in adjacent two-stage with the leakage fluid dram sealing lid；
The upper surface of described filtration container is provided with the leachy circular groove of band, is provided with the air valve cap hinged with described filtration container upper end face in described circular groove.
Intestinal contents flora extraction element the most according to claim 1 and 2, it is characterised in that the height of described circular blade is less than lateral wall height and the medial wall height of described annular groove of described annular groove.
4. according to the intestinal contents flora extraction element described in any one of claim 1-3, it is characterized in that, the sealing lid of described breaker is threadeded with described broken bucket, and described sealing lid is provided with female thread, and described broken bucket is provided with the external screw thread being connected with described female thread.
5. according to the intestinal contents flora extraction element described in any one of claim 1-4, it is characterized in that, be positioned at outside described annular groove and on the sidewall of described broken bucket, be evenly arranged with several L-type breach, be positioned at outside described annular groove described seal lid wall outer surface be provided with described L-type breach with the use of locating dowel.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610269238.3A CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
| CN201810213341.5A CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201810215283.XA CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201810214107.4A CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
| CN201810214744.1A CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810215282.5A CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810215284.4A CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810214745.6A CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610269238.3A CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810215284.4A Division CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810214744.1A Division CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810214107.4A Division CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
| CN201810215282.5A Division CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810214745.6A Division CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810213341.5A Division CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201810215283.XA Division CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
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| Publication Number | Publication Date |
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| CN105886382A true CN105886382A (en) | 2016-08-24 |
| CN105886382B CN105886382B (en) | 2018-04-24 |
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| CN201810213341.5A Expired - Fee Related CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201610269238.3A Expired - Fee Related CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
| CN201810215282.5A Active CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810214744.1A Active CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810215283.XA Active CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201810214745.6A Active CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810215284.4A Active CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810214107.4A Active CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
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| CN201810213341.5A Expired - Fee Related CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810215282.5A Active CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810214744.1A Active CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810215283.XA Active CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201810214745.6A Active CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810215284.4A Active CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810214107.4A Active CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
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| CN (8) | CN108330057B (en) |
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| KR102209997B1 (en) * | 2019-01-28 | 2021-01-29 | 이화여자대학교 산학협력단 | A device for producing a suspension for fecal microbiota transplantation |
| KR102234849B1 (en) | 2019-03-27 | 2021-04-01 | 주식회사 바이오뱅크힐링 | Fecal pretreatment kit for preparation of Fecal Microbiota Transplantation solution |
| CN114028867B (en) * | 2021-11-08 | 2023-06-27 | 武汉市农业科学院 | Quick extraction element of beasts and birds excrement and urine fungus crowd based on anaerobic condition |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN108410712B (en) | 2022-03-08 |
| CN108251288B (en) | 2021-10-08 |
| CN108264999A (en) | 2018-07-10 |
| CN108441412A (en) | 2018-08-24 |
| CN108330057A (en) | 2018-07-27 |
| CN108467827B (en) | 2021-12-31 |
| CN108485938B (en) | 2021-12-17 |
| CN108264999B (en) | 2022-02-11 |
| CN108467827A (en) | 2018-08-31 |
| CN108251288A (en) | 2018-07-06 |
| CN108330057B (en) | 2021-09-10 |
| CN105886382B (en) | 2018-04-24 |
| CN108441412B (en) | 2022-12-06 |
| CN108410712A (en) | 2018-08-17 |
| CN108485938A (en) | 2018-09-04 |
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