CN105886382B - A kind of intestinal contents flora extraction element - Google Patents
A kind of intestinal contents flora extraction element Download PDFInfo
- Publication number
- CN105886382B CN105886382B CN201610269238.3A CN201610269238A CN105886382B CN 105886382 B CN105886382 B CN 105886382B CN 201610269238 A CN201610269238 A CN 201610269238A CN 105886382 B CN105886382 B CN 105886382B
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- sealing cover
- broken
- broken bucket
- side wall
- annular groove
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
Abstract
The invention discloses a kind of intestinal contents flora extraction element, including breaker, filtration apparatus and negative pressure device；Breaker includes the broken bucket with sealing cover, is both provided with annular groove on the upper surface of broken bucket and the end face of sealing cover port, the circular blade being used cooperatively is provided with two annular grooves；A filter screen is provided with broken bottom of the barrel inner wall, filter screen upper surface is evenly equipped with spine projection, is crushed above filter screen and is provided with the shaft for being covered with spine projection on barrel side wall；It is provided with sealing cover with leachy circular groove, the air valve cap hinged with sealing cover is provided with circular groove；The dilution is filled with broken bucket；The liquid outlet with switch valve is provided with broken bucket lower sides；The beneficial effects of the invention are as follows：The present invention is not only easy to operate, but also combines plastic sealing bag collection fecal sample, as far as possible time of contact of fecal sample and air less, improves caprophyl survival rate and therapeutic effect.
Description
Technical field
The present invention relates to a kind of medical extraction element, more particularly to a kind of intestinal contents flora extraction element.
Background technology
Excrement transplanting be it is a kind of treat the method for disease by rebuilding intestinal flora, i.e., by the excrement of Healthy People at
Obtain beneficial to caprophyl liquid, that is, removing the flora that the parasites such as worm's ovum only remain with after reason, be then fabricated to capsulae enterosolubilis or it
Between take orally.Medical worker is either still psychological beyond affordability from vision, smell when preparing stool suspension, and thinks
Extraction process must be rapidly completed by ensureing the activity of caprophyl, since the flora overwhelming majority in enteron aisle is anaerobic bacterium, in medical matters
When personnel take stool sampl, the time of stool sampl one section of ingress of air, nothing is also difficult to guarantee very much in extraction process
The condition of oxygen, therefore current extraction equipment is it is difficult to ensure that the quality of caprophyl.
The content of the invention
The present invention provides a kind of time of contact for reducing excrement and air as far as possible, easy to operate and raising caprophyl to survive
The intestinal contents flora extraction element of rate.
The present invention is realized by following measure：A kind of intestinal contents flora extraction element, including breaker, with
The filtration apparatus of the breaker connection and the negative pressure device being connected with the filtration apparatus；
The breaker includes the broken bucket with sealing cover, the upper surface of the broken bucket and the sealing cover port
End face on be both provided with annular groove, be provided with the circular blade being used cooperatively in two annular grooves；The sealing
The madial wall of bezel ring, connected in star is highly less than the lateral wall of the sealing cover annular groove；
A filter screen is provided with the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine projection, position
In being provided with the shaft that is covered with spine projection above the filter screen on the broken barrel side wall, described shaft one end is pierced by described
Broken barrel side wall is provided with handle；
It is provided with the sealing cover with leachy circular groove, is provided with and the sealing cover in the circular groove
Hinged air valve cap；
The dilution is filled with the broken bucket；It is provided with the broken bucket lower sides and goes out liquid with switch valve
Mouthful.
The filtration apparatus includes the filtering container of sealing, and inlet is provided with the upper portion side wall of the filtering container,
The inlet is connected with the liquid outlet of the breaker, and liquid outlet, position are provided with the lower sides of the filtering container
Described filter is provided with negative pressure ventilation mouth, the inlet and the negative pressure ventilation mouth on container side wall above the liquid outlet
Between it is described filtering container in be provided with some levels filter membrane that from top to bottom aperture reduces step by step, filter membrane described in adjacent two-stage
Between have an interval, be provided with the discharge opeing with sealing cover on the side wall of the filtering container between filter membrane described in adjacent two-stage
Mouthful；
It is described filtering container upper surface be provided with leachy circular groove, be provided with the circular groove with
The hinged air valve cap in the filtering container upper end face.
The height of the circular blade is less than the lateral wall height of the annular groove and the madial wall of the annular groove
Highly.
The sealing cover of the breaker is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, described
Broken bucket is provided with the external screw thread being connected with the internal thread.
Several L-type notches are evenly arranged with the side wall of the broken bucket on the outside of the annular groove, positioned at institute
The wall outer surface for stating the sealing cover on the outside of annular groove is provided with the positioning column being used cooperatively with the L-type notch.
Preferably, the present invention provides a kind of intestinal contents flora extraction element, including breaker and breaker
The extraction tube of connection and the negative pressure device being connected with the extraction tube；The breaker includes the broken bucket with sealing cover,
Annular groove is both provided with the end face of the upper surface of the broken bucket and the sealing cover port, in two annular grooves
It is provided with the circular blade being used cooperatively；The madial wall of the sealing cover annular groove is highly less than the sealing cover annular groove
Lateral wall；
A filter screen is provided with the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine projection, position
In being provided with the shaft that is covered with spine projection above the filter screen on the broken barrel side wall, described shaft one end is pierced by described
Broken barrel side wall is provided with handle；
It is provided with the sealing cover with leachy circular groove, is provided with and the sealing cover in the circular groove
Hinged air valve cap；
The dilution is filled with the broken bucket；It is provided with the broken bucket lower sides and goes out liquid with switch valve
Mouthful.
The height of the circular blade is less than the lateral wall height of the annular groove.
The sealing cover is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, and the broken bucket is set
There is the external screw thread being connected with the internal thread；Or, L-type is provided with the side wall of the broken bucket on the outside of the annular groove
Notch, the wall outer surface of the sealing cover is provided with what is be used cooperatively with the L-type notch on the outside of the annular groove
Positioning column.
The extraction tube includes the filter membrane that tube body and some levels are connected with the inner wall sealing of the tube body, on the tube body
End is provided with inlet, and the bottom of the pipe is provided with the negative pressure ventilation mouth being connected with negative pressure device, close to the bottom of the pipe
Sealed set has a hard liquid storage box connected with the tube body on the side wall of the tube body, the filter membrane from top to bottom aperture
In the trend reduced step by step.
The material of the tube body is silica gel, soft rubber, PVC or flexible plastic, and is integrally formed；The aperture of the filter membrane is
0.0004-5.00mm。
Preferably, present invention also offers a kind of intestinal contents flora extraction element, the extraction element to include sample
Harvester and collecting bag；The sample collecting device includes the cylindrical collection container of upper end opening and is arranged on the collection
The upper cover in container upper end face, is provided with circular through hole or ellipse hole on the upper lid, is provided with down below the upper cover
Cone-shaped collection cover, the collecting hood lower port set the collecting bag；
The collecting bag is provided with seal assembly on the side wall of opening, and the seal assembly can be sleeved on the collection
In the lower port of cover；
Dilution is held in the collection container.
The seal assembly is magnetic stripe, and the collecting bag is symmetrically arranged with two magnetic stripes on the side wall of opening,
The both ends articulated connection of two magnetic stripes.
The upper surface of the collection container bends inwards to form annular stop plate, and the annular stop plate upper surface is provided with
Annular groove, the upper cover lower face are provided with the annular protrusion being used cooperatively with the annular groove.
Taking mouth is provided with the side wall of the collection container, is hinged with the side wall of the collection container and takes thing with described
Mouth is corresponding to take thing door.
Magnetic stripe one end of the collecting bag is connected with drawstring.
Preferably, invention further provides a kind of intestinal contents flora extraction element, the extraction element to include sample
Harvester and collecting bag；The sample collecting device includes the cylindrical collection container of upper end opening and is arranged on the collection
The upper cover in container upper end face, is provided with circular through hole or ellipse hole on the upper lid, is provided with down below the upper cover
Cone-shaped collection cover, the collecting hood lower port set the collecting bag；
The collecting bag is provided with seal assembly on the side wall of opening, and the seal assembly can be sleeved on the collection
In the lower port of cover；
Dilution is held in the collection container.
The seal assembly is magnetic stripe, and the collecting bag is symmetrically arranged with two magnetic stripes on the side wall of opening,
The both ends articulated connection of two magnetic stripes.
The upper surface of the collection container bends inwards to form annular stop plate, and the annular stop plate upper surface is provided with
Annular groove, the upper cover lower face are provided with the annular protrusion being used cooperatively with the annular groove.
Taking mouth is provided with the side wall of the collection container, is hinged with the side wall of the collection container and takes thing with described
Mouth is corresponding to take thing door.
Magnetic stripe one end of the collecting bag is connected with drawstring.
The extraction element further includes breaker, and the breaker includes the broken bucket with sealing cover, described broken
Annular groove is both provided with the end face of the upper surface of broken bucket and the sealing cover port, is provided with two annular grooves
The circular blade being used cooperatively；The madial wall of the sealing cover annular groove is highly less than the outside of the sealing cover annular groove
Wall；
A filter screen is provided with the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine projection, position
In being provided with the shaft that is covered with spine projection above the filter screen on the broken barrel side wall, described shaft one end is pierced by described
Broken barrel side wall is provided with handle；
It is provided with the sealing cover with leachy circular groove, is provided with and the sealing cover in the circular groove
Hinged air valve cap；
The dilution is filled with the broken bucket；It is provided with the broken bucket lower sides and goes out liquid with switch valve
Mouthful.
The height of the circular blade is less than the lateral wall height of the annular groove.
The sealing cover is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, and the broken bucket is set
There is the external screw thread being connected with the internal thread；Or, L-type is provided with the side wall of the broken bucket on the outside of the annular groove
Notch, the wall outer surface of the sealing cover is provided with what is be used cooperatively with the L-type notch on the outside of the annular groove
Positioning column.
The extraction element further includes filtration apparatus, and the filtration apparatus includes into fluid cylinder, several intermediate cylinders and goes out liquid
Tin, it is both provided with cambered surface filter screen in the intermediate cylinder and the discharge barrel；The intermediate cylinder close to upper port madial wall and
It is described go out fluid cylinder on the inner wall of upper port on be provided with annular gear edge, the cambered surface filter screen include annular backup pad and
The cambered surface supporting network of the annular backup pad lower face is arranged on, is provided with filter membrane in the mesh of the cambered surface supporting network, institute
Annular backup pad load is stated on the annular backup pad；
The intermediate cylinder upper port is provided with internal thread, and the intermediate cylinder lower port is provided with external screw thread, described into fluid cylinder
Lower port is provided with the external screw thread used with the intermediate cylinder screw-internal thread fit, it is described go out fluid cylinder upper port be provided with it is described
The internal thread that intermediate cylinder screw-internal thread fit uses；Several described intermediate cylinders are connected through a screw thread；
It is described go out fluid cylinder lower end be provided with liquid outlet, it is described go out fluid cylinder side wall on be provided with negative pressure ventilation mouth；
It is described into being provided with fluid cylinder upper surface with leachy circular groove, be provided with the circular groove with it is described
The hinged air valve cap of sealing cover.
Several flexible cleaning balls are placed in the cambered surface filter screen.
The beneficial effects of the invention are as follows：The present invention is not only easy to operate, but also combines plastic sealing bag collection fecal sample,
Lack the time of contact of fecal sample and air as far as possible, improve caprophyl survival rate and therapeutic effect；In use, at the first time will be strong
The fecal sample of health people is put into plastic sealing bag, keep excrement be in oxygen-free environment, then hermetic bag is placed on fill with it is dilute
Release on the upper surface of broken bucket of liquid, then cover sealing cover, sealing cover moves downward, circular blade and sealing when broken bucket
The circular blade of lid can cut in half hermetic bag when being in contact, and the half containing fecal sample is automatically fallen into broken bucket,
Crank handle drives shaft to rotate, and the spine projection in shaft is relatively moved with the spine projection on filter screen, hermetic bag
Scratched rapidly, excrement obtains removing the fecal liquid of large granular impurity, open liquid outlet through dilution and by filter screen filtration
Switch valve, fecal liquid is discharged in the filtration apparatus that negative pressure vacuumizes, after fecal liquid fully enters filtration apparatus,
Valve is turned off the switch, opens the air valve cap in filtering container upper end face, fecal liquid finally obtains intestinal beneficial after multistage filtering
Flora.
Brief description of the drawings
Fig. 1 is the structure diagram of the embodiment of the present invention 1.
Fig. 2 is the structure diagram that bucket is crushed in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 3 is the structure diagram of breaker sealing cover in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 4 is another structure diagram that bucket is crushed in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 5 is the structure diagram of filtration apparatus in the embodiment of the present invention 1.
Fig. 6 is the structure diagram of air valve cap in the embodiment of the present invention 1.
Fig. 7 is the structure diagram of the embodiment of the present invention 2.
Fig. 8 is the structure diagram of filtration apparatus in the embodiment of the present invention 2.
Fig. 9 is the structure diagram of harvester in the embodiment of the present invention 3 and embodiment 4.
Figure 10 is the structure diagram of collecting bag in the embodiment of the present invention 3 and embodiment 4.
Figure 11 is the schematic diagram of harvester use state in the embodiment of the present invention 3 and embodiment 4.
Figure 12 is the structure diagram of harvester and filtration apparatus in the embodiment of the present invention 4.
Figure 13 is the structure diagram of filtration apparatus in the embodiment of the present invention 4.
In figure, 1, broken bucket；2nd, filter screen；3rd, bearing；4th, shaft；5th, spine projection；6th, sealing cover；7th, air valve cap；8、
Handle；9th, liquid outlet；10th, connecting tube；11st, inlet；12nd, container is filtered；13rd, negative pressure ventilation mouth；14th, liquid outlet；15th, outer spiral shell
Line；16th, the lateral wall of annular groove；17th, circular blade；18th, annular groove madial wall；19th, L-type notch；20th, filter membrane；21、
Leakage fluid dram；22nd, stomata；23rd, air valve cap；24th, seal protuberance；25th, extraction tube；26th, hard liquid storage box；27th, collection container；28、
Dilution；29th, collecting bag；30th, collecting hood；31st, annular protrusion；32nd, annular stop plate；33rd, upper cover；34th, taking mouth；35th, draw
Rope；36th, articulated shaft；37th, magnetic stripe；38th, edge on collecting bag；39th, latch；40th, ellipse hole；41st, thing door is taken；42nd, into fluid cylinder；
43rd, intermediate cylinder；44th, fluid cylinder is gone out；45th, annular gear edge；46th, annular backup pad；47th, flexible cleaning ball；48th, cambered surface supporting network.
Embodiment
In order to clarify the technical characteristics of the invention, below by embodiment, this programme is illustrated.
Embodiment 1
Referring to Fig. 1-Fig. 6, a kind of intestinal contents flora extraction element, including breaker, be connected with breaker
Filtration apparatus and the negative pressure device being connected with filtration apparatus；
Breaker includes the broken bucket 1 with sealing cover 6, on the end face for 6 port of upper surface and sealing cover for crushing bucket 1
Be both provided with annular groove, the circular blade 17 being used cooperatively be provided with two annular grooves, i.e., in broken 1 upper surface of bucket with
The circular blade 17 being used cooperatively is provided with the port end face of sealing cover 6, plays the role of cut-out；6 annular groove of sealing cover
18 height of madial wall be less than the lateral wall 16 of 6 annular groove of sealing cover, the cut-off parts inside broken bucket 1 can smoothly be fallen into
In broken bucket 1；The height of circular blade 17 is less than 16 height of lateral wall of annular groove and the height of madial wall 18 of annular groove.
A filter screen 2 is provided with 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine projection 5, was located at
The shaft 4 for being covered with spine projection 5 is provided with 1 side wall of broken bucket of the top of strainer 2, the both ends of shaft 4 are horizontally disposed by bearing 3
On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by broken 1 side wall of bucket and is provided with handle 8；
The circular groove with stomata 22 is provided with sealing cover 6, the gas hinged with sealing cover 6 is provided with circular groove
Valve deck 7（Or 23 in figure）, air valve cap 7（Or 23 in figure）On be provided with seal protuberance 24 corresponding with stomata 22；
Dilution 28 is filled with broken bucket 1；The liquid outlet 14 with switch valve is provided with 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with
The external screw thread 15 of internal thread connection；Alternatively, several L-types are evenly arranged with the side wall of broken bucket 1 on the outside of annular groove
Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove
Column.
Filtration apparatus includes the filtering container 12 of sealing, filters and is provided with inlet 11 in the upper portion side wall of container 12, into
Liquid mouth 11 is connected by connecting tube 10 with the liquid outlet 9 of breaker, is filtered and is provided with liquid outlet on the lower sides of container 12
14, filtered above liquid outlet 14 on 12 side wall of container and be provided with negative pressure ventilation mouth 13, inlet 11 and negative pressure ventilation mouth 13
Between filter in container 12 and be provided with some levels filter membrane 20 that from top to bottom aperture reduces step by step, adjacent double-filtration film 20 it
Between have an interval, filtered between adjacent double-filtration film 20 and be provided with the leakage fluid dram 21 with sealing cover on the side wall of container 12；
The upper surface of filtering container 12 is provided with the circular groove with stomata 22, is provided with circular groove and holds with filtering
The hinged air valve cap 23 in 12 upper surface of device, is provided with seal protuberance 24 corresponding with stomata 22 on air valve cap 23.Wherein, negative pressure fills
The prior art is set to, details are not described herein.
In use, the fecal sample of Healthy People is put into plastic sealing bag at the first time, excrement is kept to be in anaerobic ring
In border, then hermetic bag is placed on the upper surface for the broken bucket 1 for filling with dilution 28, then covers sealing cover 6, sealing cover 6
Move downward, hermetic bag can be cut in half when the circular blade of the circular blade and sealing cover 6 that crush bucket 1 is in contact,
Half containing fecal sample is automatically fallen into broken bucket 1, and crank handle 8 drives shaft 4 to rotate, the spine projection 5 in shaft 4
Relatively moved with the spine projection 5 on filter screen 2, hermetic bag is scratched rapidly, and excrement is through diluting and passing through 2 mistake of filter screen
Filter, obtains removing the fecal liquid of large granular impurity, opens the switch valve of liquid outlet, fecal liquid is discharged to negative pressure and is vacuumized
Filtration apparatus in, after fecal liquid fully enters filtration apparatus, turn off the switch valve, open the gas of 12 upper surface of filtering container
Valve deck 23, fecal liquid finally obtain intestinal beneficial flora after multistage filtering.
Embodiment 2
Referring to Fig. 2, Fig. 3, Fig. 4, Fig. 7 and Fig. 8, the present invention provides a kind of intestinal contents flora extraction element, including
Breaker, the extraction tube 25 being connected with breaker and the negative pressure device being connected with extraction tube 25；Breaker includes carrying
The broken bucket 1 of sealing cover 6, is both provided with annular groove, two rings on the end face of 6 port of upper surface and sealing cover of broken bucket 1
The circular blade 17 being used cooperatively is provided with connected in star, i.e., is set in broken 1 upper surface of bucket and in the port end face of sealing cover 6
There is the circular blade 17 being used cooperatively, play the role of cut-out；18 height of madial wall of 6 annular groove of sealing cover is less than sealing cover
The lateral wall 16 of 6 annular grooves, crushing the cut-off parts inside bucket 1 can smoothly fall into broken bucket 1；The height of circular blade 17
Degree is less than 16 height of lateral wall of annular groove and the height of madial wall 18 of annular groove.
A filter screen 2 is provided with 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine projection 5, was located at
The shaft 4 for being covered with spine projection 5 is provided with 1 side wall of broken bucket of the top of strainer 2, the both ends of shaft 4 are horizontally disposed by bearing 3
On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by broken 1 side wall of bucket and is provided with handle 8；
The circular groove with stomata 22 is provided with sealing cover 6, the gas hinged with sealing cover 6 is provided with circular groove
Valve deck 7（Or 23 in figure）, air valve cap 7（Or 23 in figure）On be provided with seal protuberance 24 corresponding with stomata 22；
Dilution 28 is filled with broken bucket 1；The liquid outlet 14 with switch valve is provided with 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with
The external screw thread 15 of internal thread connection；Alternatively, several L-types are evenly arranged with the side wall of broken bucket 1 on the outside of annular groove
Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove
Column.
Extraction tube 25 includes the filter membrane 20 that tube body and the inner wall sealing of some levels and tube body connect, and tube body upper end is provided with
Inlet 11, the liquid outlet 9 of breaker are connected with inlet 11, and bottom of the pipe is provided with the negative pressure being connected with negative pressure device and takes out
Gas port 13, sealed set has a hard liquid storage box 26 connected with tube body, filter membrane 20 on the side wall of bottom of the pipe tube body
From top to bottom aperture is in the trend reduced step by step.
The material of tube body is silica gel, soft rubber, PVC or flexible plastic, and the material of 26 box of hard liquid storage is rigid plastics, hard
The upper port of liquid storage box 26 is integrally formed with tube body；The aperture of filter membrane 20 is 0.0004-5.00mm.
In use, the fecal sample of Healthy People is put into plastic sealing bag at the first time, excrement is kept to be in anaerobic ring
In border, then hermetic bag is placed on the upper surface for the broken bucket 1 for filling with dilution 28, then covers sealing cover 6, sealing cover 6
Move downward, hermetic bag can be cut in half when the circular blade of the circular blade and sealing cover 6 that crush bucket 1 is in contact,
Half containing fecal sample is automatically fallen into broken bucket 1, and crank handle 8 drives shaft 4 to rotate, the spine projection 5 in shaft 4
Relatively moved with the spine projection 5 on filter screen 2, hermetic bag is scratched rapidly, and excrement is through diluting and passing through 2 mistake of filter screen
Filter, obtains removing the fecal liquid of large granular impurity, opens the switch valve of liquid outlet, fecal liquid is discharged to negative pressure and is vacuumized
Filtration apparatus in, after fecal liquid fully enters filtration apparatus, turn off the switch valve, fecal liquid after multistage filtering most
Intestinal beneficial flora is obtained eventually.
Embodiment 3
Referring to Fig. 9, Figure 10 and Figure 11, an embodiment of the present invention provides a kind of intestinal contents flora extraction element, extraction
Device includes sample collecting device and collecting bag 29；Sample collecting device includes the cylindrical collection container 27 of upper end opening and sets
The upper cover 33 in 27 upper surface of collection container is put, is provided with circular through hole or ellipse hole 40 in upper cover 33, under upper cover 33
Side is provided with back taper collecting hood 30, and 30 lower port of collecting hood sets collecting bag 29；
Collecting bag 29 is close to opening（Along 38 on collecting bag）Side wall on be provided with seal assembly, seal assembly can be sleeved on
In the lower port of collecting hood 30；Dilution 28 is held in collection container.
Seal assembly is magnetic stripe 37, and collecting bag 29 is symmetrically arranged with two magnetic stripes 37, two magnetic on the side wall of opening
The both ends of bar 37 are articulated and connected by articulated shaft 36.
The upper surface of collection container 27 bends inwards to form annular stop plate 32, and 32 upper surface of annular stop plate is provided with ring
Connected in star, 33 lower face of upper cover are provided with the annular protrusion 31 being used cooperatively with annular groove.
Taking mouth 34 is provided with the side wall of collection container 27, it is right with taking mouth 34 to be hinged with the side wall of collection container 27
That answers takes thing door 41.Graduation mark is provided with the inner wall of collection container 27, ensures the liquid level just contact sleeve of dilution 28
Collecting bag 29 on collecting hood 30, magnetic stripe 37 enable to collecting bag to be just sleeved on collecting hood 39 and do not fall just, when
When having the excrement to enter collecting bag, gravity can cause collecting bag 39 to move downward.
37 one end of magnetic stripe of collecting bag 29 is connected with drawstring 35 by lock 39.
In use, dilution 28 will be held to graduation mark in collection container 27,33 side of upper cover for packaging collecting bag 29 is existed
On collection container 27, the health worker of sample to be collected is sitting in upper cover 33, when there is excrement to enter collecting bag, collecting bag due to
Gravity increase can cause collecting bag 39 to move downward, and when departing from collecting hood 30, magnetic stripe 37 attracts rapidly and in the pressure of dilution
The gas in collecting bag is excluded under power as far as possible, improves the anaerobic quality in collecting bag 39.
Embodiment 4
Referring to Fig. 2-Fig. 4 and Fig. 9-Figure 13, an embodiment of the present invention provides a kind of intestinal contents flora extraction element,
The extraction element includes sample collecting device and collecting bag 29；The cylinder collection that sample collecting device includes upper end opening is held
Device 27 and the upper cover 33 for being arranged on 27 upper surface of collection container, are provided with circular through hole or ellipse hole 40 in upper cover 33, on
The lower section of lid 33 is provided with back taper collecting hood 30, and 30 lower port of collecting hood sets collecting bag 29；
Collecting bag 29 is provided with seal assembly on the side wall of opening, and seal assembly can be sleeved on the lower end of collecting hood 30
On mouth；Dilution 28 is held in collection container.
Seal assembly is magnetic stripe 37, and collecting bag 29 is symmetrically arranged with two magnetic stripes 37, two magnetic on the side wall of opening
The both ends articulated connection of bar 37.
The upper surface of collection container 27 bends inwards to form annular stop plate 32, and 32 upper surface of annular stop plate is provided with ring
Connected in star, 33 lower face of upper cover are provided with the annular protrusion 31 being used cooperatively with annular groove.
Taking mouth 34 is provided with the side wall of collection container 27, it is right with taking mouth 34 to be hinged with the side wall of collection container 27
That answers takes thing door 41.
37 one end of magnetic stripe of collecting bag 29 is connected with drawstring 35 by lock 39.
The extraction element further includes breaker, and breaker includes the broken bucket 1 with sealing cover 6, crushes bucket 1
Annular groove is both provided with the end face of 6 port of upper surface and sealing cover, the ring being used cooperatively is provided with two annular grooves
Shape blade 17, that is, crush in 1 upper surface of bucket and be provided with the circular blade 17 being used cooperatively in the port end face of sealing cover 6, rise
To the effect of cut-out 29 seal of collecting bag；18 height of madial wall of 6 annular groove of sealing cover is less than 6 annular groove of sealing cover
Lateral wall 16, crushing the cut-out collecting bag inside bucket 1 can smoothly fall into broken bucket 1；The height of circular blade 17 is less than ring
16 height of lateral wall of connected in star and the height of madial wall 18 of annular groove.
A filter screen 2 is provided with 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine projection 5, was located at
The shaft 4 for being covered with spine projection 5 is provided with 1 side wall of broken bucket of the top of strainer 2, the both ends of shaft 4 are horizontally disposed by bearing 3
On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by broken 1 side wall of bucket and is provided with handle 8；
The circular groove with stomata 22 is provided with sealing cover 6, the gas hinged with sealing cover 6 is provided with circular groove
Valve deck 7（Or 23 in figure）, air valve cap 7（Or 23 in figure）On be provided with seal protuberance 24 corresponding with stomata 22；
Dilution 28 is filled with broken bucket 1；The liquid outlet 14 with switch valve is provided with 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with
The external screw thread 15 of internal thread connection；Alternatively, several L-types are evenly arranged with the side wall of broken bucket 1 on the outside of annular groove
Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove
Column.
The extraction element further includes filtration apparatus, and the filtration apparatus is included into fluid cylinder 42,43 and of several intermediate cylinders
Go out fluid cylinder 44, cambered surface filter screen is both provided with intermediate cylinder 43 and discharge barrel 44；Intermediate cylinder 43 close to upper port madial wall and
Go out fluid cylinder 44 on the inner wall of upper port on be provided with annular gear along 45, cambered surface filter screen includes annular backup pad 46 and sets
The cambered surface supporting network 48 in 46 lower face of annular backup pad is put, filter membrane, annular branch are provided with the mesh of cambered surface supporting network 48
Fagging 46, which is carried on a shoulder pole, to be kept off along 45 in annular and is pressed on annular gear along 45 by adjacent 43 lower port of intermediate cylinder in top；
43 upper port of intermediate cylinder is provided with internal thread, and 43 lower port of intermediate cylinder is provided with external screw thread, into 42 lower port of fluid cylinder
The external screw thread used with 43 screw-internal thread fit of intermediate cylinder is provided with, the upper port for going out fluid cylinder 44 is provided with and 43 internal thread of intermediate cylinder
The external screw thread being used cooperatively；Several intermediate cylinders 43 are connected through a screw thread；
Go out 44 lower end of fluid cylinder and be provided with liquid outlet 14, go out on the side wall of fluid cylinder 44 to be provided with negative pressure ventilation mouth 13；
It is provided with into 42 upper surface of fluid cylinder with leachy circular groove, is provided with circular groove and is hinged with sealing cover
Air valve cap；Or into fluid cylinder and to go out the side wall of fluid cylinder and intermediate cylinder be soft material, such as silica gel, soft rubber, PVC or soft modelings
Material.Several flexible cleaning balls 47 are placed in cambered surface filter screen, flexible cleaning ball 47 is covered with the sphere of thorn for softwood matter and surface,
Flexible cleaning ball 47 is the prior art, and details are not described herein.
In use, dilution 28 will be held to graduation mark in collection container 27,33 side of upper cover for packaging collecting bag 29 is existed
On collection container 27, the health worker of sample to be collected is sitting in upper cover 33, when there is excrement to enter collecting bag, collecting bag due to
Gravity increase can cause collecting bag 39 to move downward, and when departing from collecting hood 30, magnetic stripe 37 attracts rapidly and in the pressure of dilution
The gas in collecting bag is excluded under power as far as possible, improves the anaerobic quality in collecting bag 39.Then, hermetic bag is placed on fill with it is dilute
Release on the upper surface of broken bucket 1 of liquid 28, then cover sealing cover 6, sealing cover 6 moves downward, when the circular blade of broken bucket 1
Hermetic bag can be cut in half when being in contact with the circular blade of sealing cover 6, the half containing fecal sample is automatically fallen into brokenly
In broken bucket 1, crank handle 8 drives shaft 4 to rotate, and with the spine projection 5 on filter screen 2 phase occurs for the spine projection 5 in shaft 4
To movement, hermetic bag is scratched rapidly, and excrement obtains removing the excrement of large granular impurity through diluting and being filtered by filter screen 2
Liquid, opens the switch valve of liquid outlet, fecal liquid is discharged in the filtration apparatus that negative pressure vacuumizes, and treats fecal liquid whole
Into after filtration apparatus, valve is turned off the switch, fecal liquid finally obtains intestinal beneficial flora after multistage filtering.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on, should all be included in the protection scope of the present invention.
Claims (4)
1. a kind of intestinal contents flora extraction element, including breaker, the filtration apparatus that is connected with the breaker and
The negative pressure device being connected with the filtration apparatus；
The breaker includes the end of the broken bucket with sealing cover, the upper surface of the broken bucket and the sealing cover port
Annular groove is both provided with face, the circular blade being used cooperatively is provided with two annular grooves；The sealing bezel ring,
The madial wall of connected in star is highly less than the lateral wall of the sealing cover annular groove；
A filter screen is provided with the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine projection, positioned at institute
State and be provided with the shaft for being covered with spine projection above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken
Barrel side wall is provided with handle；
It is provided with the sealing cover with leachy circular groove, is provided with the circular groove and is hinged with the sealing cover
Air valve cap；
Dilution is filled with the broken bucket；The liquid outlet with switch valve is provided with the broken bucket lower sides；
The filtration apparatus includes the filtering container of sealing, and inlet is provided with the upper portion side wall of the filtering container, described
Inlet is connected with the liquid outlet of the breaker, liquid outlet is provided with the lower sides of the filtering container, positioned at institute
State and be provided with negative pressure ventilation mouth on the filtering container side wall above liquid outlet, between the inlet and the negative pressure ventilation mouth
Some levels filter membrane that from top to bottom aperture reduces step by step is provided with the filtering container, between filter membrane described in adjacent two-stage
There is an interval, the leakage fluid dram with sealing cover is provided with the side wall of the filtering container between filter membrane described in adjacent two-stage；
It is described filtering container upper surface be provided with leachy circular groove, be provided with the circular groove with it is described
Filter the hinged air valve cap in container upper end face.
2. intestinal contents flora extraction element according to claim 1, it is characterised in that the height of the circular blade
Less than the lateral wall height of the annular groove and the madial wall height of the annular groove.
3. intestinal contents flora extraction element according to claim 1, it is characterised in that the sealing of the breaker
Lid is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, and the broken bucket is provided with to be connected with the internal thread
The external screw thread connect.
4. intestinal contents flora extraction element according to claim 1, it is characterised in that outside the annular groove
Crushed described in side and be evenly arranged with several L-type notches on the side wall of bucket, the sealing cover on the outside of the annular groove
Wall outer surface is provided with the positioning column being used cooperatively with the L-type notch.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810214745.6A CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810215284.4A CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810215283.XA CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201810214744.1A CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810213341.5A CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201610269238.3A CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
| CN201810215282.5A CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810214107.4A CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610269238.3A CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
Related Child Applications (7)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810214745.6A Division CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810215283.XA Division CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201810215284.4A Division CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810213341.5A Division CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201810215282.5A Division CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810214744.1A Division CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810214107.4A Division CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105886382A CN105886382A (en) | 2016-08-24 |
| CN105886382B true CN105886382B (en) | 2018-04-24 |
Family
ID=56704771
Family Applications (8)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810215284.4A Active CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810213341.5A Expired - Fee Related CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201810215282.5A Active CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810215283.XA Active CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201610269238.3A Expired - Fee Related CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
| CN201810214745.6A Active CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810214744.1A Active CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810214107.4A Active CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
Family Applications Before (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810215284.4A Active CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810213341.5A Expired - Fee Related CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201810215282.5A Active CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201810215283.XA Active CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810214745.6A Active CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810214744.1A Active CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810214107.4A Active CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
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| CN (8) | CN108264999B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2020039433A1 (en) * | 2018-08-20 | 2020-02-27 | The Medical Research, Infrastructure and Health Services Fund of the Tel Aviv Medical Center | Fecal processing device and methods |
| KR102209997B1 (en) * | 2019-01-28 | 2021-01-29 | 이화여자대학교 산학협력단 | A device for producing a suspension for fecal microbiota transplantation |
| KR102234849B1 (en) | 2019-03-27 | 2021-04-01 | 주식회사 바이오뱅크힐링 | Fecal pretreatment kit for preparation of Fecal Microbiota Transplantation solution |
| CN112226344B (en) * | 2020-10-14 | 2023-08-18 | 大连大学 | Anaerobic extraction and encapsulation intestinal flora integrated machine |
| CN114028867B (en) * | 2021-11-08 | 2023-06-27 | 武汉市农业科学院 | Quick extraction element of beasts and birds excrement and urine fungus crowd based on anaerobic condition |
| CN115261271B (en) * | 2022-08-01 | 2023-12-12 | 厦门承葛生物科技有限公司 | High-flux separation culture and screening method for intestinal flora |
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- 2016-04-27 CN CN201810213341.5A patent/CN108330057B/en not_active Expired - Fee Related
- 2016-04-27 CN CN201810215282.5A patent/CN108441412B/en active Active
- 2016-04-27 CN CN201810215283.XA patent/CN108251288B/en active Active
- 2016-04-27 CN CN201610269238.3A patent/CN105886382B/en not_active Expired - Fee Related
- 2016-04-27 CN CN201810214745.6A patent/CN108467827B/en active Active
- 2016-04-27 CN CN201810214744.1A patent/CN108485938B/en active Active
- 2016-04-27 CN CN201810214107.4A patent/CN108410712B/en active Active
Also Published As
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|---|---|
| CN108410712A (en) | 2018-08-17 |
| CN108330057B (en) | 2021-09-10 |
| CN108264999B (en) | 2022-02-11 |
| CN108441412B (en) | 2022-12-06 |
| CN108251288A (en) | 2018-07-06 |
| CN108264999A (en) | 2018-07-10 |
| CN108251288B (en) | 2021-10-08 |
| CN108485938A (en) | 2018-09-04 |
| CN108330057A (en) | 2018-07-27 |
| CN108485938B (en) | 2021-12-17 |
| CN108467827B (en) | 2021-12-31 |
| CN108410712B (en) | 2022-03-08 |
| CN108467827A (en) | 2018-08-31 |
| CN108441412A (en) | 2018-08-24 |
| CN105886382A (en) | 2016-08-24 |
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