CN108410712A - A kind of caprophyl anaerobic extraction element and extracting method - Google Patents
A kind of caprophyl anaerobic extraction element and extracting method Download PDFInfo
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- CN108410712A CN108410712A CN201810214107.4A CN201810214107A CN108410712A CN 108410712 A CN108410712 A CN 108410712A CN 201810214107 A CN201810214107 A CN 201810214107A CN 108410712 A CN108410712 A CN 108410712A
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- sealing cover
- broken
- broken bucket
- caprophyl
- side wall
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
Abstract
The invention discloses a kind of caprophyl anaerobic extraction element and extracting methods, and wherein extraction element includes breaker, the extraction tube being connect with breaker and the negative pressure device being connect with extraction tube；Breaker includes the broken bucket with sealing cover, is both provided with annular groove on the upper surface of broken bucket and the end face of sealing cover port, the circular blade being used cooperatively is provided in two annular grooves；It is provided with a filter screen on broken bottom of the barrel inner wall, filter screen upper surface is evenly equipped with spine protrusion, is crushed above filter screen on barrel side wall and is provided with the shaft for being covered with spine protrusion, shaft one end is pierced by broken barrel side wall and is provided with handle；Dilution is filled in broken bucket；It is provided with the liquid outlet with switch valve on broken bucket lower sides.The beneficial effects of the invention are as follows：The present invention is not only easy to operate, but also plastic sealing bag is combined to acquire fecal sample, as far as possible time of contact of fecal sample and air less, improves caprophyl survival rate and therapeutic effect.
Description
This case is divisional application, original application it is entitled：A kind of intestinal contents flora extraction element, original application
The applying date is：2016-04-27, original application application No. is：201610269238.3.
Technical field
The present invention relates to a kind of medical extraction element more particularly to a kind of caprophyl anaerobic extraction element and extracting methods.
Background technology
Excrement transplanting is a kind of method for treating disease by rebuilding intestinal flora, i.e., by the excrement of Healthy People at
Obtained after reason beneficial to caprophyl liquid, that is, remove the parasites such as worm's ovum and only retain useful flora, be then fabricated to capsulae enterosolubilis or it
Between take orally.Medical worker is either still psychological beyond affordability from vision, smell when preparing stool suspension, and thinks
Ensure that extraction process must be rapidly completed in the activity of caprophyl, since the flora overwhelming majority in enteron aisle is anaerobic bacterium, in medical matters
When personnel take stool sampl, the time of stool sampl one section of ingress of air, nothing is also difficult to guarantee very much in extraction process
The condition of oxygen, thus current extraction equipment it is difficult to ensure that caprophyl quality.
Invention content
The present invention provides a kind of time of contact reducing excrement and air as far as possible, easy to operate and raising caprophyls to survive
The caprophyl anaerobic extraction element and extracting method of rate.
The present invention is realized by following measure：A kind of intestinal contents flora extraction element, including breaker, with
The filter device of the breaker connection and the negative pressure device being connect with the filter device；
The breaker includes the broken bucket with sealing cover, the end of the upper surface of the broken bucket and the sealing cover port
It is both provided with annular groove on face, the circular blade being used cooperatively is provided in two annular grooves；The sealing bezel ring,
The madial wall height of connected in star is less than the lateral wall of the sealing cover annular groove；
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, is located at institute
It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken
Barrel side wall is provided with handle；
It is provided on the sealing cover with leachy circular groove, is provided in the circular groove hinged with the sealing cover
Air valve cap；
The dilution is filled in the broken bucket；It is provided with the liquid outlet with switch valve on the broken bucket lower sides.
The filter device includes the filtering container of sealing, and inlet is provided in the upper portion side wall of the filtering container,
The inlet is connect with the liquid outlet of the breaker, and liquid outlet, position are provided on the lower sides of the filtering container
It is provided with negative pressure ventilation mouth, the inlet and the negative pressure ventilation mouth on the filtering container side wall above the liquid outlet
Between be provided with several grades filter membrane that from top to bottom aperture reduces step by step in the filtering container, filter membrane described in adjacent two-stage
Between have an interval, be provided with the drain with sealing cover on the side wall of the filtering container between filter membrane described in adjacent two-stage
Mouthful；
It is described filtering container upper surface be provided with leachy circular groove, be provided in the circular groove with it is described
Filter the hinged air valve cap in container upper end face.
The height of the circular blade is less than the madial wall of the lateral wall height and the annular groove of the annular groove
Highly.
The sealing cover of the breaker is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, described
Broken bucket is provided with the external screw thread being connect with the internal thread.
Several L-type notches are evenly arranged on the side wall of the broken bucket on the outside of the annular groove, are located at institute
The wall outer surface for stating the sealing cover on the outside of annular groove is provided with the positioning column being used cooperatively with the L-type notch.
Preferably, it the present invention provides a kind of caprophyl anaerobic extraction element, including breaker, is connect with breaker
Extraction tube and the negative pressure device being connect with the extraction tube；The breaker includes the broken bucket with sealing cover, described broken
It is both provided with annular groove on the end face of the upper surface of broken bucket and the sealing cover port, is provided in two annular grooves
The circular blade being used cooperatively；The madial wall height of the sealing cover annular groove is less than the outside of the sealing cover annular groove
Wall；
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, is located at institute
It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken
Barrel side wall is provided with handle；
It is provided on the sealing cover with leachy circular groove, is provided in the circular groove hinged with the sealing cover
Air valve cap；
The dilution is filled in the broken bucket；It is provided with the liquid outlet with switch valve on the broken bucket lower sides.
The height of the circular blade is less than the lateral wall height of the annular groove.
The sealing cover is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, the broken bucket setting
There is the external screw thread being connect with the internal thread；Or, being provided with L-type on the side wall of the broken bucket on the outside of the annular groove
Notch, the wall outer surface of the sealing cover, which is provided with, on the outside of the annular groove is used cooperatively with the L-type notch
Positioning column.
The extraction tube includes tube body and the filter membrane that several grades are connect with the inner wall sealing of the tube body, on the tube body
End is provided with inlet, and the bottom of the pipe is provided with the negative pressure ventilation mouth being connect with negative pressure device, close to the bottom of the pipe
Sealed set has a hard liquid storage box being connected to the tube body on the side wall of the tube body, the filter membrane from top to bottom aperture
In the trend reduced step by step.
The material of the tube body is silica gel, soft rubber, PVC or flexible plastic, and is integrally formed；The aperture of the filter membrane is
0.0004-5.00mm。
Preferably, the present invention also provides a kind of intestinal contents flora extraction element, the extraction element includes sample
Harvester and collecting bag；The sample collecting device includes the cylindrical collection container of upper end opening and is arranged in the acquisition
The upper cover in container upper end face is provided with circular through hole or ellipse hole on the upper lid, is provided with down below the upper cover
The collecting bag is arranged in cone-shaped collection cover, the collecting hood lower port；
The collecting bag is provided with seal assembly on the side wall of opening, and the seal assembly can be sleeved on the collecting hood
In lower port；
Dilution is held in the collection container.
The seal assembly is magnetic stripe, and the collecting bag is symmetrical arranged on the side wall of opening there are two the magnetic stripe,
The both ends articulated connection of two magnetic stripes.
The upper surface of the collection container bends inwards to form annular stop plate, and the annular stop plate upper surface is provided with
Annular groove, the upper cover lower face are provided with the annular protrusion being used cooperatively with the annular groove.
It is provided with taking mouth on the side wall of the collection container, is hinged on the side wall of the collection container and takes object with described
Mouth is corresponding to take object door.
Magnetic stripe one end of the collecting bag is connected with drawstring.
Preferably, invention further provides a kind of intestinal contents flora extraction element, the extraction element includes sample
Harvester and collecting bag；The sample collecting device includes the cylindrical collection container of upper end opening and is arranged in the acquisition
The upper cover in container upper end face is provided with circular through hole or ellipse hole on the upper lid, is provided with down below the upper cover
The collecting bag is arranged in cone-shaped collection cover, the collecting hood lower port；
The collecting bag is provided with seal assembly on the side wall of opening, and the seal assembly can be sleeved on the collecting hood
In lower port；
Dilution is held in the collection container.
The seal assembly is magnetic stripe, and the collecting bag is symmetrical arranged on the side wall of opening there are two the magnetic stripe,
The both ends articulated connection of two magnetic stripes.
The upper surface of the collection container bends inwards to form annular stop plate, and the annular stop plate upper surface is provided with
Annular groove, the upper cover lower face are provided with the annular protrusion being used cooperatively with the annular groove.
It is provided with taking mouth on the side wall of the collection container, is hinged on the side wall of the collection container and takes object with described
Mouth is corresponding to take object door.
Magnetic stripe one end of the collecting bag is connected with drawstring.
The extraction element further includes breaker, and the breaker includes the broken bucket with sealing cover, described broken
It is both provided with annular groove on the end face of the upper surface of broken bucket and the sealing cover port, is provided in two annular grooves
The circular blade being used cooperatively；The madial wall height of the sealing cover annular groove is less than the outside of the sealing cover annular groove
Wall；
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, is located at institute
It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken
Barrel side wall is provided with handle；
It is provided on the sealing cover with leachy circular groove, is provided in the circular groove hinged with the sealing cover
Air valve cap；
The dilution is filled in the broken bucket；It is provided with the liquid outlet with switch valve on the broken bucket lower sides.
The height of the circular blade is less than the lateral wall height of the annular groove.
The sealing cover is threadedly coupled with the broken bucket, and the sealing cover is provided with internal thread, the broken bucket setting
There is the external screw thread being connect with the internal thread；Or, being provided with L-type on the side wall of the broken bucket on the outside of the annular groove
Notch, the wall outer surface of the sealing cover, which is provided with, on the outside of the annular groove is used cooperatively with the L-type notch
Positioning column.
The extraction element further includes filter device, and the filter device includes into fluid cylinder, several intermediate cylinders and going out liquid
Tin, it is both provided with cambered surface filter screen in the intermediate cylinder and the discharge barrel；The intermediate cylinder close to upper port madial wall and
It is described go out fluid cylinder on the inner wall of upper port on be provided with annular gear edge, the cambered surface filter screen include annular backup pad and
Cambered surface supporting network in the annular backup pad lower face is set, is provided with filter membrane in the mesh of the cambered surface supporting network, institute
Annular backup pad load is stated on the annular backup pad；
The intermediate cylinder upper port is provided with internal thread, and the intermediate cylinder lower port is provided with external screw thread, described into fluid cylinder lower end
Mouth is provided with the external screw thread used with the intermediate cylinder screw-internal thread fit, it is described go out fluid cylinder upper port be provided with and the centre
The internal thread that cylinder screw-internal thread fit uses；Several described intermediate cylinders are connected through a screw thread；
It is described go out fluid cylinder lower end be provided with liquid outlet, it is described go out fluid cylinder side wall on be provided with negative pressure ventilation mouth；
It is described into being provided on fluid cylinder upper surface with leachy circular groove, be provided with and the sealing in the circular groove
Cover hinged air valve cap.
Several flexible cleaning balls are placed in the cambered surface filter screen.
The beneficial effects of the invention are as follows：The present invention is not only easy to operate, but also plastic sealing bag is combined to acquire fecal sample,
The time of contact for lacking fecal sample and air as far as possible, improve caprophyl survival rate and therapeutic effect；In use, will be good at the first time
The fecal sample of health people is put into plastic sealing bag, keep excrement be in oxygen-free environment, then hermetic bag is placed on fill with it is dilute
It releases on the upper surface of broken bucket of liquid, then covers sealing cover, sealing cover moves downward, the circular blade when broken bucket and sealing
The circular blade of lid can cut in half hermetic bag when being in contact, and the half containing fecal sample is automatically fallen into broken bucket,
Crank handle drives shaft rotation, the spine protrusion in shaft to be relatively moved with the spine protrusion on filter screen, hermetic bag
It is scratched rapidly, excrement obtains the fecal liquid of removal large granular impurity, open liquid outlet through dilution and by filter screen filtration
Switch valve, fecal liquid is discharged in the filter device that negative pressure vacuumizes, after fecal liquid fully enters filter device,
Valve is turned off the switch, the air valve cap in filtering container upper end face is opened, fecal liquid finally obtains intestinal beneficial after multistage filtering
Flora.
Description of the drawings
Fig. 1 is the structural schematic diagram of the embodiment of the present invention 1.
Fig. 2 is the structural schematic diagram that bucket is crushed in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 3 is the structural schematic diagram of breaker sealing cover in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 4 is another structural schematic diagram that bucket is crushed in the embodiment of the present invention 1, embodiment 2 and embodiment 4.
Fig. 5 is the structural schematic diagram of filter device in the embodiment of the present invention 1.
Fig. 6 is the structural schematic diagram of air valve cap in the embodiment of the present invention 1.
Fig. 7 is the structural schematic diagram of the embodiment of the present invention 2.
Fig. 8 is the structural schematic diagram of filter device in the embodiment of the present invention 2.
Fig. 9 is the structural schematic diagram of harvester in the embodiment of the present invention 3 and embodiment 4.
Figure 10 is the structural schematic diagram of collecting bag in the embodiment of the present invention 3 and embodiment 4.
Figure 11 is the schematic diagram of harvester use state in the embodiment of the present invention 3 and embodiment 4.
Figure 12 is the structural schematic diagram of harvester and filter device in the embodiment of the present invention 4.
Figure 13 is the structural schematic diagram of filter device in the embodiment of the present invention 4.
In figure, 1, broken bucket；2, filter screen；3, bearing；4, shaft；5, spine protrusion；6, sealing cover；7, air valve cap；8、
Handle；9, liquid outlet；10, connecting tube；11, inlet；12, container is filtered；13, negative pressure ventilation mouth；14, liquid outlet；15, outer spiral shell
Line；16, the lateral wall of annular groove；17, circular blade；18, annular groove madial wall；19, L-type notch；20, filter membrane；21、
Leakage fluid dram；22, stomata；23, air valve cap；24, seal protuberance；25, extraction tube；26, hard liquid storage box；27, collection container；28、
Dilution；29, collecting bag；30, collecting hood；31, annular protrusion；32, annular stop plate；33, upper cover；34, taking mouth；35, it draws
Rope；36, articulated shaft；37, magnetic stripe；38, collecting bag upper edge；39, it latches；40, ellipse hole；41, object door is taken；42, into fluid cylinder；
43, intermediate cylinder；44, go out fluid cylinder；45, annular gear edge；46, annular backup pad；47, flexible cleaning ball；48, cambered surface supporting network.
Specific implementation mode
In order to clarify the technical characteristics of the invention, below by specific implementation mode, this programme is illustrated.
Embodiment 1
Referring to Fig. 1-Fig. 6, a kind of intestinal contents flora extraction element, including breaker, the filtering that is connect with breaker
Device and the negative pressure device being connect with filter device；
Breaker includes the broken bucket 1 with sealing cover 6, is all provided on the end face of 6 port of upper surface and sealing cover of broken bucket 1
It is equipped with annular groove, the circular blade 17 being used cooperatively is provided in two annular grooves, i.e., in broken 1 upper surface of bucket and is sealed
It is provided with the circular blade 17 being used cooperatively in the port end face of lid 6, plays the role of cut-out；6 annular groove of sealing cover it is interior
18 height of side wall is less than the lateral wall 16 of 6 annular groove of sealing cover, and the cut-off parts inside broken bucket 1 can smoothly fall into broken
In bucket 1；The height of circular blade 17 is less than 16 height of lateral wall of annular groove and 18 height of madial wall of annular groove.
It is provided with a filter screen 2 in 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine protrusion 5, was located at
2 top of strainer, which is crushed on 1 side wall of bucket, is provided with the shaft 4 for being covered with spine protrusion 5, and the both ends of shaft 4 are horizontally disposed by bearing 3
On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by 1 side wall of broken bucket and is provided with handle 8；
It is provided with the circular groove with stomata 22 on sealing cover 6, the air valve cap hinged with sealing cover 6 is provided in circular groove
7（Or 23 in figure）, air valve cap 7（Or 23 in figure）On be provided with seal protuberance corresponding with stomata 22 24；
Dilution 28 is filled in broken bucket 1；It is provided with the liquid outlet 14 with switch valve on 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with
The external screw thread 15 of internal thread connection；It is evenly arranged with several L-types alternatively, being crushed on the outside of annular groove on the side wall of bucket 1
Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove
Column.
Filter device includes the filtering container 12 of sealing, filters and is provided with inlet 11 in the upper portion side wall of container 12, into
Liquid mouth 11 is connect by connecting tube 10 with the liquid outlet 9 of breaker, is filtered and is provided with liquid outlet on the lower sides of container 12
14, it is filtered above liquid outlet 14 on 12 side wall of container and is provided with negative pressure ventilation mouth 13, inlet 11 and negative pressure ventilation mouth 13
Between be provided with several grades filter membrane 20 that from top to bottom aperture reduces step by step in filtering container 12, adjacent double-filtration film 20 it
Between have an interval, be provided with the leakage fluid dram 21 with sealing cover on the side wall of filtering container 12 between adjacent double-filtration film 20；
The upper surface of filtering container 12 is provided with the circular groove with stomata 22, is provided in circular groove and filtering container 12
The hinged air valve cap 23 in upper surface is provided with seal protuberance corresponding with stomata 22 24 on air valve cap 23.Wherein, negative pressure device is
The prior art, details are not described herein.
In use, the fecal sample of Healthy People is put into plastic sealing bag at the first time, excrement is kept to be in anaerobic ring
In border, then hermetic bag is placed on the upper surface for the broken bucket 1 for filling with dilution 28, then covers sealing cover 6, sealing cover 6
It moves downward, hermetic bag can be cut in half when the circular blade of the circular blade of broken bucket 1 and sealing cover 6 is in contact,
Half containing fecal sample is automatically fallen into broken bucket 1, and crank handle 8 drives shaft 4 to rotate, the spine protrusion 5 in shaft 4
It is relatively moved with the spine protrusion 5 on filter screen 2, hermetic bag is scratched rapidly, and excrement is through diluting and passing through 2 mistake of filter screen
Filter obtains the fecal liquid of removal large granular impurity, opens the switch valve of liquid outlet, fecal liquid, which is discharged to negative pressure, to be vacuumized
Filter device in, after fecal liquid fully enters filter device, turn off the switch valve, open the gas of 12 upper surface of filtering container
Valve deck 23, fecal liquid finally obtain intestinal beneficial flora after multistage filtering.
Embodiment 2
Referring to Fig. 2, Fig. 3, Fig. 4, Fig. 7 and Fig. 8, the present invention provides a kind of caprophyl anaerobic extraction element, including breaker, with
The extraction tube 25 of breaker connection and the negative pressure device being connect with extraction tube 25；Breaker includes broken with sealing cover 6
Broken bucket 1 is crushed on the end face of 6 port of upper surface and sealing cover of bucket 1 and is both provided with annular groove, setting in two annular grooves
There is a circular blade 17 being used cooperatively, i.e., is used cooperatively with being provided in the port end face of sealing cover 6 in broken 1 upper surface of bucket
Circular blade 17, plays the role of cut-out；18 height of madial wall of 6 annular groove of sealing cover is less than 6 annular groove of sealing cover
Lateral wall 16, being crushed the cut-off parts inside bucket 1 can smoothly fall into broken bucket 1；The height of circular blade 17 is less than annular
16 height of lateral wall of groove and 18 height of madial wall of annular groove.
It is provided with a filter screen 2 in 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine protrusion 5, was located at
2 top of strainer, which is crushed on 1 side wall of bucket, is provided with the shaft 4 for being covered with spine protrusion 5, and the both ends of shaft 4 are horizontally disposed by bearing 3
On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by 1 side wall of broken bucket and is provided with handle 8；
It is provided with the circular groove with stomata 22 on sealing cover 6, the air valve cap hinged with sealing cover 6 is provided in circular groove
7（Or 23 in figure）, air valve cap 7（Or 23 in figure）On be provided with seal protuberance corresponding with stomata 22 24；
Dilution 28 is filled in broken bucket 1；It is provided with the liquid outlet 14 with switch valve on 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with
The external screw thread 15 of internal thread connection；It is evenly arranged with several L-types alternatively, being crushed on the outside of annular groove on the side wall of bucket 1
Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove
Column.
Extraction tube 25 includes the filter membrane 20 that tube body and the inner wall sealing of several grades and tube body connect, and tube body upper end is provided with
The liquid outlet 9 of inlet 11, breaker is connect with inlet 11, and bottom of the pipe is provided with the negative pressure being connect with negative pressure device and takes out
Gas port 13, sealed set has a hard liquid storage box 26 being connected to tube body, filter membrane 20 on the side wall of bottom of the pipe tube body
From top to bottom aperture is in the trend reduced step by step.
The material of tube body is silica gel, soft rubber, PVC or flexible plastic, and the material of 26 box of hard liquid storage is rigid plastics, hard
The upper port of liquid storage box 26 is integrally formed with tube body；The aperture of filter membrane 20 is 0.0004-5.00mm.
In use, the fecal sample of Healthy People is put into plastic sealing bag at the first time, excrement is kept to be in anaerobic ring
In border, then hermetic bag is placed on the upper surface for the broken bucket 1 for filling with dilution 28, then covers sealing cover 6, sealing cover 6
It moves downward, hermetic bag can be cut in half when the circular blade of the circular blade of broken bucket 1 and sealing cover 6 is in contact,
Half containing fecal sample is automatically fallen into broken bucket 1, and crank handle 8 drives shaft 4 to rotate, the spine protrusion 5 in shaft 4
It is relatively moved with the spine protrusion 5 on filter screen 2, hermetic bag is scratched rapidly, and excrement is through diluting and passing through 2 mistake of filter screen
Filter obtains the fecal liquid of removal large granular impurity, opens the switch valve of liquid outlet, fecal liquid, which is discharged to negative pressure, to be vacuumized
Filter device in, after fecal liquid fully enters filter device, turn off the switch valve, fecal liquid after multistage filtering most
Intestinal beneficial flora is obtained eventually.
Embodiment 3
Referring to Fig. 9, Figure 10 and Figure 11, an embodiment of the present invention provides a kind of intestinal contents flora extraction element, extraction elements
Including sample collecting device and collecting bag 29；Sample collecting device includes that the cylindrical collection container 27 of upper end opening and setting exist
The upper cover 33 of 27 upper surface of collection container is provided with circular through hole or ellipse hole 40 in upper cover 33, and the lower section of upper cover 33 is set
It is equipped with back taper collecting hood 30, collecting bag 29 is arranged in 30 lower port of collecting hood；
Collecting bag 29 is close to opening（Collecting bag upper edge 38）Side wall on be provided with seal assembly, seal assembly can be sleeved on collection
In the lower port of cover 30；Dilution 28 is held in collection container.
Seal assembly is magnetic stripe 37, and collecting bag 29 is arranged symmetrically on the side wall of opening, and there are two magnetic stripe 37, two magnetic
The both ends of item 37 are articulated and connected by articulated shaft 36.
The upper surface of collection container 27 bends inwards to form annular stop plate 32, and 32 upper surface of annular stop plate is provided with ring
Connected in star, 33 lower face of upper cover are provided with the annular protrusion 31 being used cooperatively with annular groove.
Taking mouth 34 is provided on the side wall of collection container 27, it is right with taking mouth 34 to be hinged on the side wall of collection container 27
That answers takes object door 41.It is provided with graduation mark on the inner wall of collection container 27, ensures the liquid level just contact sleeve of dilution 28
Collecting bag 29 on collecting hood 30, magnetic stripe 37 enable to collecting bag to be just sleeved on collecting hood 39 and do not fall just, when
When having excrement to enter collecting bag, gravity can cause collecting bag 39 to move downward.
37 one end of magnetic stripe of collecting bag 29 is connected with drawstring 35 by lock 39.
In use, dilution 28 will be held to graduation mark in collection container 27,33 side of upper cover for packaging collecting bag 29 is existed
On collection container 27, the health worker of sample to be collected is sitting in upper cover 33, when there is excrement to enter collecting bag, collecting bag due to
Gravity increase can cause collecting bag 39 to move downward, and when being detached from collecting hood 30, magnetic stripe 37 attracts rapidly and in the pressure of dilution
The gas in collecting bag is excluded under power as possible, improves the anaerobic quality in collecting bag 39.
Embodiment 4
It is described an embodiment of the present invention provides a kind of intestinal contents flora extraction element referring to Fig. 2-Fig. 4 and Fig. 9-Figure 13
Extraction element includes sample collecting device and collecting bag 29；Sample collecting device includes the cylindrical collection container 27 of upper end opening
With the upper cover 33 in 27 upper surface of collection container is set, circular through hole or ellipse hole 40, upper cover 33 are provided in upper cover 33
Lower section be provided with back taper collecting hood 30, collecting bag 29 is arranged in 30 lower port of collecting hood；
Collecting bag 29 is provided with seal assembly on the side wall of opening, and seal assembly can be sleeved on the lower port of collecting hood 30
On；Dilution 28 is held in collection container.
Seal assembly is magnetic stripe 37, and collecting bag 29 is arranged symmetrically on the side wall of opening, and there are two magnetic stripe 37, two magnetic
The both ends of item 37 are articulated and connected.
The upper surface of collection container 27 bends inwards to form annular stop plate 32, and 32 upper surface of annular stop plate is provided with ring
Connected in star, 33 lower face of upper cover are provided with the annular protrusion 31 being used cooperatively with annular groove.
Taking mouth 34 is provided on the side wall of collection container 27, it is right with taking mouth 34 to be hinged on the side wall of collection container 27
That answers takes object door 41.
37 one end of magnetic stripe of collecting bag 29 is connected with drawstring 35 by lock 39.
The extraction element further includes breaker, and breaker includes the broken bucket 1 with sealing cover 6, is crushed bucket 1
It is both provided with annular groove on the end face of 6 port of upper surface and sealing cover, the ring being used cooperatively is provided in two annular grooves
Shape blade 17 is crushed in 1 upper surface of bucket and is provided with the circular blade 17 being used cooperatively in the port end face of sealing cover 6, rises
To the effect of cut-out 29 seal of collecting bag；18 height of madial wall of 6 annular groove of sealing cover is less than 6 annular groove of sealing cover
Lateral wall 16, being crushed the cut-out collecting bag inside bucket 1 can smoothly fall into broken bucket 1；The height of circular blade 17 is less than ring
16 height of lateral wall of connected in star and 18 height of madial wall of annular groove.
It is provided with a filter screen 2 in 1 bottom interior wall of broken bucket, 2 upper surface of filter screen is evenly equipped with spine protrusion 5, was located at
2 top of strainer, which is crushed on 1 side wall of bucket, is provided with the shaft 4 for being covered with spine protrusion 5, and the both ends of shaft 4 are horizontally disposed by bearing 3
On the side wall of broken bucket 1, the wherein one end of shaft 4 is pierced by 1 side wall of broken bucket and is provided with handle 8；
It is provided with the circular groove with stomata 22 on sealing cover 6, the air valve cap hinged with sealing cover 6 is provided in circular groove
7（Or 23 in figure）, air valve cap 7（Or 23 in figure）On be provided with seal protuberance corresponding with stomata 22 24；
Dilution 28 is filled in broken bucket 1；It is provided with the liquid outlet 14 with switch valve on 1 lower sides of broken bucket.
The sealing cover 6 of breaker is threadedly coupled with broken bucket 1, and sealing cover is provided with internal thread, broken bucket 1 be provided with
The external screw thread 15 of internal thread connection；It is evenly arranged with several L-types alternatively, being crushed on the outside of annular groove on the side wall of bucket 1
Notch 19, the wall outer surface of the sealing cover is provided with the positioning being used cooperatively with L-type notch 19 on the outside of annular groove
Column.
The extraction element further includes filter device, and the filter device includes into fluid cylinder 42, several intermediate cylinders 43 and going out liquid
Cylinder 44 is both provided with cambered surface filter screen in intermediate cylinder 43 and discharge barrel 44；Intermediate cylinder 43 close to upper port madial wall and go out liquid
Cylinder 44 on the inner wall of upper port on be provided with annular gear along 45, cambered surface filter screen includes that annular backup pad 46 and setting exist
The cambered surface supporting network 48 of 46 lower face of annular backup pad is provided with filter membrane, annular backup pad in the mesh of cambered surface supporting network 48
46 loads keep off along 45 in annular and are pressed on annular gear along 45 by adjacent 43 lower port of intermediate cylinder in top；
43 upper port of intermediate cylinder is provided with internal thread, and 43 lower port of intermediate cylinder is provided with external screw thread, is arranged into 42 lower port of fluid cylinder
There is the external screw thread used with 43 screw-internal thread fit of intermediate cylinder, the upper port for going out fluid cylinder 44 is provided with and 43 screw-internal thread fit of intermediate cylinder
The external screw thread used；Several intermediate cylinders 43 are connected through a screw thread；
Go out 44 lower end of fluid cylinder and be provided with liquid outlet 14, goes out on the side wall of fluid cylinder 44 to be provided with negative pressure ventilation mouth 13；
It is provided on into 42 upper surface of fluid cylinder with leachy circular groove, the gas hinged with sealing cover is provided in circular groove
Valve deck；Or into fluid cylinder and to go out the side wall of fluid cylinder and intermediate cylinder be soft material, such as silica gel, soft rubber, PVC or flexible plastic.
Several flexible cleaning balls 47 are placed in cambered surface filter screen, flexible cleaning ball 47 is the sphere that softwood matter and surface are covered with thorn, soft
Property rotating wash bowl 47 be the prior art, details are not described herein.
In use, dilution 28 will be held to graduation mark in collection container 27,33 side of upper cover for packaging collecting bag 29 is existed
On collection container 27, the health worker of sample to be collected is sitting in upper cover 33, when there is excrement to enter collecting bag, collecting bag due to
Gravity increase can cause collecting bag 39 to move downward, and when being detached from collecting hood 30, magnetic stripe 37 attracts rapidly and in the pressure of dilution
The gas in collecting bag is excluded under power as possible, improves the anaerobic quality in collecting bag 39.Then, hermetic bag is placed on fill with it is dilute
It releases on the upper surface of broken bucket 1 of liquid 28, then covers sealing cover 6, sealing cover 6 moves downward, when the circular blade of broken bucket 1
Hermetic bag can be cut in half when being in contact with the circular blade of sealing cover 6, the half containing fecal sample is automatically fallen into brokenly
In broken bucket 1, crank handle 8 drives shaft 4 to rotate, and with the spine protrusion 5 on filter screen 2 phase occurs for the spine protrusion 5 in shaft 4
To movement, hermetic bag is scratched rapidly, and excrement obtains the excrement of removal large granular impurity through diluting and being filtered by filter screen 2
Liquid opens the switch valve of liquid outlet, fecal liquid is discharged in the filter device that negative pressure vacuumizes, and waits for fecal liquid whole
Into after filter device, valve is turned off the switch, fecal liquid finally obtains intestinal beneficial flora after multistage filtering.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of caprophyl anaerobic extraction element, which is characterized in that the extraction tube that is connect including breaker, with breaker and with
The negative pressure device of the extraction tube connection；The breaker includes the broken bucket with sealing cover, the upper end of the broken bucket
It is both provided with annular groove on the end face of face and the sealing cover port, is provided with and is used cooperatively in two annular grooves
Circular blade；The madial wall height of the sealing cover annular groove is less than the lateral wall of the sealing cover annular groove；
A filter screen is provided on the broken bottom of the barrel inner wall, the filter screen upper surface is evenly equipped with spine protrusion, is located at institute
It states and is provided with the shaft for being covered with spine protrusion above filter screen on the broken barrel side wall, described shaft one end is pierced by described broken
Barrel side wall is provided with handle；
The dilution is filled in the broken bucket；It is provided with the liquid outlet with switch valve on the broken bucket lower sides.
2. caprophyl anaerobic extraction element according to claim 1, which is characterized in that be provided with gas on the sealing cover
The circular groove in hole is provided with the air valve cap hinged with the sealing cover in the circular groove.
3. caprophyl anaerobic extraction element according to claim 1 or 2, which is characterized in that the height of the circular blade is small
In the lateral wall height of the annular groove.
4. according to claim 1-3 any one of them caprophyl anaerobic extraction elements, which is characterized in that the sealing cover with it is described
Broken bucket is threadedly coupled, and the sealing cover is provided with internal thread, and the broken bucket is provided with the outer spiral shell being connect with the internal thread
Line；Or, being provided with L-type notch on the side wall of the broken bucket on the outside of the annular groove, it is located at outside the annular groove
The wall outer surface of sealing cover described in side is provided with the positioning column being used cooperatively with the L-type notch.
5. according to claim 1-3 any one of them caprophyl anaerobic extraction elements, which is characterized in that the extraction tube includes pipe
The filter membrane that body and several grades are connect with the inner wall sealing of the tube body, the tube body upper end are provided with inlet, the tube body
Lower end is provided with the negative pressure ventilation mouth being connect with negative pressure device, the sealed set on the side wall of tube body described in the bottom of the pipe
There is a hard liquid storage box being connected to the tube body, from top to bottom aperture is in the trend reduced step by step to the filter membrane.
6. caprophyl anaerobic extraction element according to claim 5, which is characterized in that the material of the tube body is silica gel, soft
Rubber, PVC or flexible plastic, and be integrally formed；The aperture of the filter membrane is 0.0004-5.00mm.
7. according to the extracting method of claim 1-6 any one of them caprophyl anaerobic extraction elements, which is characterized in that described to carry
The method is taken to be specially：The fecal sample of Healthy People is put into plastic sealing bag at the first time, excrement is kept to be in oxygen-free environment
In, then hermetic bag is placed on the upper surface for the broken bucket for filling with dilution, then covers sealing cover, sealing cover is transported downwards
It is dynamic, hermetic bag can be cut in half when the circular blade of the circular blade of broken bucket and sealing cover is in contact, contains excrement
The half of sample is automatically fallen into broken bucket, and crank handle drives shaft to rotate, in the spine protrusion and filter screen in shaft
Spine protrusion relatively moves, and hermetic bag is scratched rapidly, and excrement obtains removing big through dilution and by filter screen filtration
The fecal liquid of grain impurity, opens the switch valve of liquid outlet, fecal liquid is discharged in the filter device that negative pressure vacuumizes, is waited for
After fecal liquid fully enters filter device, valve is turned off the switch, fecal liquid finally obtains intestinal beneficial after multistage filtering
Flora.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810214107.4A CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810214107.4A CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
| CN201610269238.3A CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610269238.3A Division CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
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| CN108410712A true CN108410712A (en) | 2018-08-17 |
| CN108410712B CN108410712B (en) | 2022-03-08 |
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| CN201810213341.5A Expired - Fee Related CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201810215284.4A Active CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810214744.1A Active CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
| CN201810214107.4A Active CN108410712B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal strain extraction device and method |
| CN201810215283.XA Active CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201810214745.6A Active CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810215282.5A Active CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201610269238.3A Expired - Fee Related CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810213341.5A Expired - Fee Related CN108330057B (en) | 2016-04-27 | 2016-04-27 | Intestinal content flora extraction device and extraction method |
| CN201810215284.4A Active CN108264999B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal flora extraction device |
| CN201810214744.1A Active CN108485938B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection device and collection method for fecal strain extraction |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201810215283.XA Active CN108251288B (en) | 2016-04-27 | 2016-04-27 | Anaerobic fecal bacteria extraction device and method |
| CN201810214745.6A Active CN108467827B (en) | 2016-04-27 | 2016-04-27 | Fecal flora extraction device |
| CN201810215282.5A Active CN108441412B (en) | 2016-04-27 | 2016-04-27 | Anaerobic collection and crushing device for fecal strain extraction |
| CN201610269238.3A Expired - Fee Related CN105886382B (en) | 2016-04-27 | 2016-04-27 | A kind of intestinal contents flora extraction element |
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| CN114028867A (en) * | 2021-11-08 | 2022-02-11 | 武汉市农业科学院 | Quick extraction element of animal manure fungus crowd based on anaerobic condition |
| EP3840667A4 (en) * | 2018-08-20 | 2022-04-27 | The Medical Research, Infrastructure and Health Services Fund of the Tel Aviv Medical Center | Fecal processing device and methods |
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| KR102209997B1 (en) * | 2019-01-28 | 2021-01-29 | 이화여자대학교 산학협력단 | A device for producing a suspension for fecal microbiota transplantation |
| KR102234849B1 (en) | 2019-03-27 | 2021-04-01 | 주식회사 바이오뱅크힐링 | Fecal pretreatment kit for preparation of Fecal Microbiota Transplantation solution |
| CN112033743A (en) * | 2020-07-16 | 2020-12-04 | 上海市东方医院（同济大学附属东方医院） | Parkinson's excrement and urine liquid extraction element |
| CN112226344B (en) * | 2020-10-14 | 2023-08-18 | 大连大学 | Anaerobic extraction and encapsulation intestinal flora integrated machine |
| CN115261271B (en) * | 2022-08-01 | 2023-12-12 | 厦门承葛生物科技有限公司 | High-flux separation culture and screening method for intestinal flora |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN108485938A (en) | 2018-09-04 |
| CN108264999B (en) | 2022-02-11 |
| CN108441412B (en) | 2022-12-06 |
| CN108467827B (en) | 2021-12-31 |
| CN108330057B (en) | 2021-09-10 |
| CN108251288A (en) | 2018-07-06 |
| CN105886382B (en) | 2018-04-24 |
| CN108485938B (en) | 2021-12-17 |
| CN108441412A (en) | 2018-08-24 |
| CN108251288B (en) | 2021-10-08 |
| CN108330057A (en) | 2018-07-27 |
| CN108410712B (en) | 2022-03-08 |
| CN108467827A (en) | 2018-08-31 |
| CN105886382A (en) | 2016-08-24 |
| CN108264999A (en) | 2018-07-10 |
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