A kind of compound bactericidal composition for control of plant disease
Technical field
The present invention relates to a kind of rice disease prevention and treatment compositions.
Background technique
2- amino -5- sulfydryl -1,3,4- thiadiazoles zinc (Universal Chinese character name: zinc thiazole) is a kind of the new of China's independent development
Type plant fungicide has broad spectrum antibacterial performance, has good control efficiency particularly with bacterial blight of rice, however, with
The popularization and use of recent two decades, the pathogenic bacteria of bacterial blight of rice resistance is gradually generated to zinc thiazole, cause its control efficiency
Begin to decline, to keep control efficiency, have to increase dosage in actual use, but existing research shows that zinc thiazole still
A kind of thyroid interferent, can cause the parathyroid tissue hyperplasia of experimental animal, and toxicity has apparent dosage effect phase
Guan Xing.
It is derivative that a kind of Isosorbide-5-Nitrae containing quinoxaline-pentadiene -3- ketone is disclosed in Chinese patent literature CN107602539A
Object, and disclose a series of application of derivatives in treatment tobacco mosaic virus (TMV).The document specifically discloses 1- (4- (2- quinoline
Quinoline base) phenyl) -5- (2- methoxyphenyl)-Isosorbide-5-Nitrae-pentadiene -3- ketone (A6), but disclosed in it in experimental data, chemical combination
The treatment protection of the tobacco mosaic virus (TMV) of object (A6) and passivation are below the Ningnanmycin of drug as a comparison.
Based on problem in the prior art above, compound how is formed rationally to improve existing bactericide anti-and harness the river
The effect when plant diseases such as the bacterial blight of rice becomes urgent problem to be solved in the prior art.
Summary of the invention
To solve aforementioned technical problem, under study for action it was found that disclosing seriation when using in CN107602539A
Close 1- (4- (2- quinoxalinyl) phenyl) -5- (2- the methoxyphenyl) -1,4- pentadiene -3- ketone (A6) and 2- amino-in object
5- sulfydryl -1,3 can be improved significantly when compound is made in 4- thiadiazoles zinc (I) compatibility for phytopathies such as bacterials blight of rice
Harmful control efficiency is based on this, and the invention adopts the following technical scheme:
A kind of compound bactericidal composition for control of plant disease is provided, it is characterized in that the composition is by least
A kind of auxiliary material and mass ratio are the 2- amino -5- sulfydryl -1,3,4- thiadiazoles zinc (I) and 1- (4- (2- quinoline of 1:0.15~0.25
Quinoline base) phenyl) -5- (2- methoxyphenyl) -1,4- pentadiene -3- ketone (A6) composition.
A kind of compound bactericidal composition for control of plant disease, it is characterized in that chemical combination in the composition
The mass ratio of object (I) and compound (A6) is preferably 1:0.18~0.22.
A kind of compound bactericidal composition for control of plant disease, it is characterized in that the dosage form of the composition is selected
From one of/emulsifiable concentrate, wettable powder, suspending agent, soluble powder.The gross mass of compound (I) and compound (A6)
The 15%~30% of the compound bactericidal composition gross mass is accounted for, preferred compound (I) and the gross mass of compound (A6) account for institute
State the 18%~25% of compound bactericidal composition gross mass.
A kind of compound bactericidal composition for control of plant disease, it is characterized in that the dosage form of the composition is
Suspending agent, the auxiliary material are the auxiliary material suitable for suspending agent, and the auxiliary material for suspending agent includes dispersing agent, wetting agent, collapses
Agent, binder, defoaming agent and filler are solved,
The disintegrating agent is selected from one or more of sodium chloride, sulfate, carbonate and sodium carboxymethylcellulose.
The dispersing agent is selected from sodium lignin sulfonate, calcium lignosulfonate, polycarboxylate, sodium methylene bis-naphthalene sulfonate
(NNO), APES formaldehyde condensation product, fatty alcohol polyoxyethylene ether sulfate, alkyl phenol polyoxyethylene ether fourth two
One or more of acid ester sulfonates.
The defoaming agent is selected from the representative examples of saturated aliphatic carboxylic and C10~C20 of the fatty acid ester compounded object of higher alcohols, C10~C20
One of representative examples of saturated aliphatic carboxylic ester is a variety of.
The binder is selected from one of gelatin, Arabic gum, polyvinyl alcohol, polyethylene glycol and starch or a variety of.
The wetting agent is selected from Nekal BX, EO/PO block polyether, fatty alcohol polyoxyethylene ether, fatty alcohol ethoxylate
Close one of object, Negel and fatty alcohol polyoxyethylene ether sulfate or a variety of.
It is kaolin, diatomite, bentonite, attapulgite, talcum powder, starch, glucose, sulphur that the filler, which is selected from filler,
At least one of sour sodium, precipitated calcium carbonate.The water content of the composition is 0.2~0.5%.
A kind of compound bactericidal composition for control of plant disease provided by the invention, by compound (I) and compound
(A6) compatibility is carried out with special ratios, the significant effect when preventing and treating bacterial blight of rice for obtaining composition is higher than compound
(I).It, which belongs to low toxicity compounds, and the side effect of athyreosis interference, is shown to the acute toxicity detection of compound (A6).According to
Experiment in control efficiency embodiment, compound (A6) itself are very low to the control efficiency of bacterial blight of rice.The present invention provides
A kind of compound bactericidal composition for control of plant disease, the ratio of preferred compound (I) and compound (A6) is being added
Bacteriostatic activity when for preventing and treating bacterial blight of rice is greatly improved in the case where minority specioz (A6), so as to
On the basis of reaching same control efficiency, the usage amount of compound (I) is reduced, and then reduces the dirt of compound (I) to environment
It contaminates and to adverse effect caused by user's health.And in recipe determination it was found that compound (I) and compound (A6)
Ratio is there is preferred range, when the two proportion is 1:0.15~0.25, can play more apparent effect, it is higher or
Lower compound (A6) additional amount can not all generate the effect, and the ratio in compound (I) and compound (A6) is preferred
1:0.18~0.22 when, improve prevention and treatment bacterial blight of rice effect become apparent.
Specific embodiment
A kind of compound bactericidal composition for control of plant disease provided by the invention, is prepared into suspending agent, by changing
Close object (I), compound (A6) and auxiliary material composition.The formula of the composition is respectively as shown in Examples 1 to 5 in following table, simultaneously
It prepares comparative example 1~5 (percentage shown in table is the mass percent for accounting for composition gross mass, disregards moisture):
The preparation method of the composition are as follows:
1) each component is added to dosage bunker according to the proportion and adds water to pulpous state, is stirred evenly;
2) coarse crushing is carried out using high-speed shearing machine, being crushed to granularity is 100~200 mesh;
3) sand mill is used to be sanded to granularity as 8000~10000 mesh after coarse crushing;
4) it is sent into pressure spray drying tower drying after being sanded, is granulated, obtains water content and meets the requirements (0.2%~0.5%)
Partial size be the hollow aperture spherical particle of 80~100 purposes.As suspending agent.
The dispersing agent is selected from NNO, and wetting agent is selected from Nekal BX, and disintegrating agent is selected from sodium chloride, and binder is selected from poly- second
Glycol 2000, defoaming agent are selected from hexadecylic acid, and filler is kaolin.
The compound (I) and compound (A6) used in experiment is provided by Tianjin Kai Yin Science and Technology Ltd., and content is equal
>=98%, compound (I) lot number is 20170904.Compound (A6) lot number is 20170901.
Control efficiency experiment
The compound (I) and compound (A6) for connecing bacterium method for measuring test different proportion with field leaf-cutting are to the white leaf of rice
The control efficiency of blight, experimental procedure are as follows
1, using the rice leaf spot bacteria strain of Zhejiang Academy of Agricultural Science plant protection and institute of microbiology
(Xanthomonasoryzaepv.oryzae (Xoo)), culture become 109After the suspension of a/ml, with the dissecting scissors for being moistened with bacterium solution
3~4cm of rice blade tip is subtracted, 20 rice leaves of every group of selection, 7 days sprays prevent and treat after connecing bacterium, examine or check and compare within 10 days after administration
The control efficiency (calculating control efficiency with Spot expansion) and use Excel that group is compared carry out t inspection, water used to experimental result
Rice varieties are middle morning 39 (authorization number is that rice 2009039 is examined in Zhejiang)
2, grouping see the table below (means ± s, n=20) with effect situation with administration
By above-mentioned each group drug, experimental group 1~7 and control 1/2 are by suspending agent made from each embodiment according to effective component
Final concentration 200ppm is diluted, and control 3 is diluted to compound (A6) final concentration 40ppm, and positive controls are using comparative example 5
Suspending agent (active substance) is diluted with water 1000 times.
The experimental results showed that the compound bactericidal composition that the embodiment of the present invention 1~5 provides, passes through preferred specific chemical combination
The ratio of object (I) and compound (A6), the effect compared with compound alone (I), when improving for bacterial blight of rice prevention and treatment
Fruit., the control efficiency compared with using 1 group of control of compound (I) of experimental group 1~5 is significantly increased (P < 0.05).And it adopts
With the experimental group 6/7 of comparative example 1/2, although the effective component ratio with experimental group 1~5 is closer to, its control efficiency is
Started to begin to decline to compare 1 group without significant difference.And 2/3 group of control that compound (A6) is administered alone shows to be applied alone
Compound (A6), almost without control efficiency, especially compares 3 groups to bacterial blight of rice, control efficiency and positive controls
Also without significant difference, illustrate that compound (I) and compound (A6) produces synergistic effect under specific preferred proportion, preventing and treating
Unexpected effect is produced when rice bacterial leaf spot.