CN108226264A - A kind of dribble dropping buffer solution gradient operating system and its operating method - Google Patents
A kind of dribble dropping buffer solution gradient operating system and its operating method Download PDFInfo
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- CN108226264A CN108226264A CN201810098856.5A CN201810098856A CN108226264A CN 108226264 A CN108226264 A CN 108226264A CN 201810098856 A CN201810098856 A CN 201810098856A CN 108226264 A CN108226264 A CN 108226264A
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- buffer solution
- capillary
- operating system
- liquid
- droping head
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- 239000007853 buffer solution Substances 0.000 title claims abstract description 67
- 238000011017 operating method Methods 0.000 title claims abstract description 11
- 239000007788 liquid Substances 0.000 claims abstract description 59
- 238000005251 capillar electrophoresis Methods 0.000 claims abstract description 16
- 238000002474 experimental method Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 239000004615 ingredient Substances 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 14
- 239000002699 waste material Substances 0.000 claims description 6
- 239000000872 buffer Substances 0.000 claims description 5
- 239000008213 purified water Substances 0.000 claims description 4
- 238000001802 infusion Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 2
- -1 at least four Substances 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 description 10
- 238000000926 separation method Methods 0.000 description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005370 electroosmosis Methods 0.000 description 2
- 238000004451 qualitative analysis Methods 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of dribble dropping buffer solution gradient operating systems, the liquid storage bottle for being used to store buffer solution including at least four, liquid storage bottle ratio control unit passes through connection, ratio control unit is connected with flow controlling unit, peristaltic pump in flow controlling unit is connected with liquid droping head, and liquid droping head is equipped with the jack that Pt electrodes and capillary are inserted into.The operating method of dribble dropping buffer solution gradient operating system, capillary is cleaned, is activated, booting preheating, appropriate buffer solution ingredient is selected according to experimental subjects, determines initial buffer solution formula, is prepared, clean Pt electrodes, sample introduction, after connecting capillary electrophoresis, bubble in pipeline, setting flow, gradient parameter and working voltage value etc. are removed, start high pressure, data are recorded, after the completion of record, repeating experiment should be since walking third；Experiment finishes, with pure water rinsing dribble dropping buffer solution gradient operating system, cleaning electrode and capillary.The configuration of the present invention is simple, it is easy to operate.
Description
Technical field
Patent of the present invention is related to Capillary Electrophoresis, and in particular to a kind of dribble dropping buffer solution gradient operating system and its behaviour
Make method.
Background technology
Existing capillary electrophoresis system uses the single buffer solution method of operation to carry out sample separation analysis substantially, very not
It is big but include the separation of the true complex sample of the close component of property analysis, this kind of sample conducive to nature difference between most components
Complicated components, according to single buffer solution, disengaging time increases significantly, and chromatographic peak is widened aggravation naturally, and separation efficiency declines.I.e.
Take a small number of capillary electrophoresis methods for employing gradient buffer solution, also by the way of buffer solution is replaced manually,
It is cumbersome so that the stability of separation is greatly reduced, and is unfavorable for qualitative and quantitative analysis.
Invention content
The present invention provides a kind of dribble dropping buffer solution gradient operating system and its operating methods, solve gradient buffer
The capillary electrophoresis method of solution takes by the way of buffer solution is replaced manually, is cumbersome so that the stability of separation
The technical issues of being greatly reduced.
Technical scheme is as follows：
A kind of dribble dropping buffer solution gradient operating system, including at least four for storing the liquid storage bottle of buffer solution,
Liquid storage bottle is connect with adjusting the ratio control unit of solution proportion by the first conduit, and the solution in each liquid storage bottle is controlled in ratio
It is mixed in unit by both certainty ratios, ratio control unit is connected by the second conduit with flow controlling unit, flow control list
The output terminal of peristaltic pump in member is connected with liquid droping head, and the liquid droping head is equipped with for the Pt electrodes and hair in capillary electrophoresis
The jack that tubule is inserted into, the end lower section that oozes of the liquid droping head are equipped with drip collector unit.
Further, the liquid droping head is packaged type liquid droping head, and is provided with rubber stopper at the jack of the liquid droping head,
Pt electrodes and capillary enter after passing through rubber stopper in liquid droping head.
Further, the drip collector unit top is provided with a diameter of one centimetre of waste pipe, the waste liquid
Tube at one end is connected with drip collector unit, and the other end connects the funnel of an opening face liquid droping head upward；The ratio
Control unit includes low pressure proportioning valve and for controlling the circuit board of low pressure proportioning valve and control panel.
The operating method of the dribble dropping buffer solution gradient operating system, carries out according to the following steps：
The first step：Capillary is cleaned, is activated；
Second step：Booting preheating；
Third walks：Appropriate buffer solution ingredient is selected according to experimental subjects, single composition buffer solution to be mixed is distinguished
Suitable concentration is made into be stored in reservoir；
4th step：It determines initial buffer solution formula, prepares, and initial buffer solution is filled with capillary and capillary electricity
In the initial buffer solution cup of the test side of swimming instrument；
5th step：Pt electrodes, sample introduction are cleaned, Pt electrodes and capillary are inserted into liquid droping head；Capillary electrophoresis is installed
The initial buffer liquid cup of detector end；
6th step：After this dribble dropping buffer solution gradient operating system connection capillary electrophoresis, by wriggling pump discharge tune
It to 5ml/min, runs 1~5 minute, removes bubble in pipeline；The operation of 6th step can be after the completion of second step using purified water as fortune
The operation of row solution is primary, but must complete the 6th step after the completion of the 5th step；
7th step：Flow, gradient parameter and working voltage value are set；
8th step：Start high pressure, record data；
9th step：After the completion of record, repeating experiment should be since walking third；
Tenth step：Experiment finishes, with pure water rinsing dribble dropping buffer solution gradient operating system 5~10 minutes, flow 0.5
~1.0ml/min, cleaning electrode and capillary.
Further, the 6th step is：Dribble dropping buffer solution gradient operating system is connected, wriggling pump discharge is adjusted to 5ml/
Min is run 2~4 minutes, removes bubble in infusion pipeline system.
Further, the tenth step is：Experiment finishes, with pure water rinsing dribble dropping buffer solution gradient operating system 5~10
Minute, 0.6~0.9ml/min of flow, cleaning electrode and capillary.
Beneficial effects of the present invention：Dribble dropping buffer solution gradient operating system and its operating method proposed by the present invention,
Make it possible buffer solution during Capillary Electrophoresis it is automatic replace or ratio controllably transformation from this, substantially increases hair
The scope of application of cons electrophoresis method, moreover it is possible to improve separation efficiency, shorten analysis time so that the separation of Capillary Electrophoresis is stablized
Property greatly improves, and is more favorable for the qualitative and quantitative analysis of complex sample.Dribble dropping buffer solution gradient operating system is compact, and ties
Structure is simple easy to maintain and controls, and can individually produce and be used with the collocation of existing capillary electrophoresis, can expand existing capillary significantly
The function of electrophoresis tube instrument.The capillary electrophoresis system operating method of dribble dropping buffer solution gradient operation, simple and practicable, safe nothing
It is effective to can guarantee that experimental result is stablized for evil.
Description of the drawings
Fig. 1 is the structural diagram of the present invention.
Fig. 2 is the structure diagram that Fig. 1 is applied in combination with electrocapillary phoresis instrument
Component label instructions：Liquid storage bottle 1, the first conduit 2, ratio control unit 3, the second conduit 5, flow controlling unit 6,
Peristaltic pump 7, third conduit 8, liquid droping head 9, Pt electrodes 10, capillary 11, drip collector unit 12, rubber pad 13, waste liquid are led
Pipe 14, funnel 15.
Specific embodiment
It elaborates below in conjunction with the accompanying drawings to the present invention.
A kind of dribble dropping buffer solution gradient operating system as shown in Figs. 1-2, it is molten for storing buffering including at least four
The various solution prepared needed for buffer solution are stored in each liquid storage bottle 1 by the liquid storage bottle 1 of liquid, and liquid storage bottle 1 is with adjusting solution ratio
The ratio control unit 3 of example is connected by the first conduit 2, and the solution in each liquid storage bottle 1 presses set ratio in ratio control unit 3
Example mixing, it can be achieved that replace buffer ratio, without independent redistribution at any time.Ratio control unit 3 by the second conduit 5 with
Flow controlling unit 6 is connected, and the output terminal of the peristaltic pump 7 in flow controlling unit 6 is connected with liquid droping head 9, the peristaltic pump 7
Using the micro peristaltic pump of high-precision low pulse, the liquid droping head 9 is equipped with Pt electrodes 10 and capillary for 11 electrophoresis apparatus of capillary
The jack that pipe 11 is inserted into, the end lower section that oozes of the liquid droping head 9 are equipped with drip collector unit 12.Due to 11 electrophoresis apparatus of capillary
The high voltage that provides of Pt electrodes 10 so that electroosmotic flow is generated in capillary 11；The buffer solution of liquid droping head 9 is flowed through in electroosmotic flow
It acts on next part to flow into capillary 11, a part flows into drip collector unit 12 by liquid droping head 9, because in liquid droping head 9
Buffer solution replaced, therefore the buffer solution for entering capillary 11 also converts accordingly ceaselessly automatic in proportion so that
The runtime buffer solution being separated by electrophoresis in detection process for capillary 11 is not stopping to replace, and it is controllable to realize buffer solution gradient
The purpose of transformation, improves separative efficiency, shortens analysis time.Capillary 11 will be in liquid droping head 9 in Fig. 1, Pt electrodes 10
Slightly stretch out liquid droping head 9, and lean out than capillary 11 longer, be conducive to electrophoresis progress, be also beneficial to dropping liquid smoothly into
Row.
Further, the liquid droping head 9 is packaged type liquid droping head, and is provided with rubber at the jack of the liquid droping head 9
Plug, Pt electrodes 10 and capillary 11 enter after passing through rubber stopper in liquid droping head 9.It can prevent the buffer solution in liquid droping head 9 from overflowing, together
When can fix Pt electrodes 10 and capillary 11.
Further, the waste liquid for a diameter of one centimetre being provided between the liquid droping head 9 and drip collector unit 12 is led
Pipe, the ratio control unit 3 include low pressure proportioning valve and the circuit board for controlling low pressure proportioning valve.
The operating method of the dribble dropping buffer solution gradient operating system carries out according to the following steps：
The first step：Capillary 11 is cleaned, is activated.
Second step：Booting preheating.
Third walks：Appropriate buffer solution ingredient is selected according to experimental subjects, single composition buffer solution to be mixed is distinguished
It is made into suitable concentration to be stored in reservoir, e.g., buffer solution ingredient is NaH2PO3-Na2HPO3, with NaOH tune pH, then respectively will
NaH2PO3, Na2HPO3, NaOH are made into suitable concentration and are stored in A/B/C liquid storage bottles 1, and D bottles are often placed purified water or dilute hydrochloric acid
Solution.
4th step：It determines initial buffer solution formula, prepares, and initial buffer solution is filled with capillary 11 and capillary
In the initial buffer solution cup of the test side of 11 electrophoresis apparatuses；
5th step：Pt electrodes 10, sample introduction are cleaned, Pt electrodes 10 and capillary 11 are inserted into liquid droping head 9；Capillary is installed
The initial buffer liquid cup of 11 electrophoresis apparatus detector ends.11 electrophoresis apparatus of capillary is connect with dribble dropping buffer solution gradient operating system
It uses.
6th step：After this dribble dropping buffer solution gradient operating system connection 11 electrophoresis apparatus of capillary, by 7 flow of peristaltic pump
5ml/min is adjusted to, is run 1~5 minute, removes bubble in pipeline；6th step operation can after the completion of second step using purified water as
It is primary to run solution operation, but the 6th step must be completed after the completion of the 5th step.
7th step：Flow, gradient parameter and working voltage value etc. are set.
8th step：Start high pressure, operation records data.
9th step：After the completion of record, repeating experiment should be since walking third.
Tenth step：Experiment finishes, with pure water rinsing dribble dropping buffer solution gradient operating system 5~10 minutes, flow 0.5
~1.0ml/min, cleaning Pt electrode 10 and capillary 11.
Further, the 6th step is：Dribble dropping buffer solution gradient operating system is connected, 7 flow of peristaltic pump is adjusted to
5ml/min is run 2~4 minutes, removes bubble in infusion pipeline system.
Further, the tenth step is：Experiment finishes, with pure water rinsing dribble dropping buffer solution gradient operating system 5~10
Minute, 0.6~0.9ml/min of flow cleans Pt electrodes 10 and capillary 11.
Finally illustrate, the above embodiments are merely illustrative of the technical solutions of the present invention and it is unrestricted, although with reference to compared with
The present invention is described in detail in good embodiment, it will be understood by those of ordinary skill in the art that, it can be to the skill of the present invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this
In the right of invention.
Claims (6)
1. a kind of dribble dropping buffer solution gradient operating system, which is characterized in that be used to store buffer solution including at least four
Liquid storage bottle, liquid storage bottle with adjust solution proportion ratio control unit connect by the first conduit, the solution in each liquid storage bottle
It being mixed in ratio control unit by both certainty ratios, ratio control unit is connected by the second conduit with flow controlling unit,
The output terminal of peristaltic pump in flow controlling unit is connected with liquid droping head, and the liquid droping head is equipped in capillary electrophoresis
The jack that Pt electrodes and capillary are inserted into, the end lower section that oozes of the liquid droping head are equipped with drip collector unit.
2. a kind of dribble dropping buffer solution gradient operating system according to claim 1, which is characterized in that the liquid droping head is
Packaged type liquid droping head, and rubber stopper is provided at the jack of the liquid droping head, Pt electrodes and capillary pass through rubber stopper laggard
Enter in liquid droping head.
3. a kind of dribble dropping buffer solution gradient operating system according to claim 1 or claim 2, which is characterized in that described to ooze
Liquid collector unit top is provided with a diameter of one centimetre of waste pipe, and described waste pipe one end connects with drip collector unit
Logical, the other end connects the funnel of an opening face liquid droping head upward；The ratio control unit includes low pressure proportioning valve and use
In the circuit board and control panel of control low pressure proportioning valve.
4. a kind of operating method of dribble dropping buffer solution gradient operating system as described in claim 1, it is characterised in that：By with
Lower step carries out：
The first step：Capillary is cleaned, is activated；
Second step：Booting preheating；
Third walks：Appropriate buffer solution ingredient is selected according to experimental subjects, single composition buffer solution to be mixed is made into respectively
Suitable concentration is stored in reservoir；
4th step：It determines initial buffer solution formula, prepares, and initial buffer solution is filled with capillary and capillary electrophoresis
Test side initial buffer solution cup in；
5th step：Pt electrodes, sample introduction are cleaned, Pt electrodes and capillary are inserted into liquid droping head；Capillary electrophoresis detection is installed
The initial buffer liquid cup at device end；
6th step：After this dribble dropping buffer solution gradient operating system connection capillary electrophoresis, wriggling pump discharge is adjusted to
5ml/min is run 1~5 minute, removes bubble in pipeline；The operation of 6th step can be after the completion of second step using purified water as operation
Solution operation is primary, but must complete the 6th step after the completion of the 5th step；
7th step：Flow, gradient parameter and working voltage value are set；
8th step：Start high pressure, record data；
9th step：After the completion of record, repeating experiment should be since walking third；
Tenth step：Experiment finishes, with pure water rinsing dribble dropping buffer solution gradient operating system 5~10 minutes, flow 0.5~
1.0ml/min, cleaning electrode and capillary.
5. a kind of operating method of dribble dropping buffer solution gradient operating system according to claim 4, which is characterized in that the
Six steps are：Dribble dropping buffer solution gradient operating system is connected, wriggling pump discharge is adjusted to 5ml/min, runs 2~4 minutes, goes
Except bubble in infusion pipeline system.
6. a kind of operating method of dribble dropping buffer solution gradient operating system according to claim 4, which is characterized in that the
Ten steps are：Experiment finishes, with pure water rinsing dribble dropping buffer solution gradient operating system 5~10 minutes, 0.6~0.9ml/ of flow
Min, cleaning electrode and capillary.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810098856.5A CN108226264B (en) | 2018-01-31 | 2018-01-31 | Dropping type buffer solution gradient operation system and operation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810098856.5A CN108226264B (en) | 2018-01-31 | 2018-01-31 | Dropping type buffer solution gradient operation system and operation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108226264A true CN108226264A (en) | 2018-06-29 |
| CN108226264B CN108226264B (en) | 2020-11-03 |
Family
ID=62670286
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810098856.5A Expired - Fee Related CN108226264B (en) | 2018-01-31 | 2018-01-31 | Dropping type buffer solution gradient operation system and operation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113454451A (en) * | 2019-02-28 | 2021-09-28 | 思拓凡瑞典有限公司 | Control of buffer preparation process |
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| US5221448A (en) * | 1990-01-25 | 1993-06-22 | Spectra-Physics Analytical, Inc. | Buffer gradient and temperature gradient capillary electrophoresis |
| CN2476817Y (en) * | 2001-04-20 | 2002-02-13 | 天润通微(天津)分析技术有限公司 | Quantitative sample feeding gradient pressured capillary electrophoresis device |
| CN1936568A (en) * | 2005-09-22 | 2007-03-28 | 上海通微分析技术有限公司 | Quantitative high-precision capillary tube electrophoresis apparatus |
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| CN104395748A (en) * | 2012-06-26 | 2015-03-04 | 安捷伦科技有限公司 | Fluidic valve with selectively switchable storage paths |
| CN204989098U (en) * | 2015-08-03 | 2016-01-20 | 李绍平 | Capillary electrophoresis and electrochromatography plug -in components |
| CN105723212A (en) * | 2013-07-08 | 2016-06-29 | 株式会社日立高新技术 | Capillary electrophoresis device |
| WO2017046783A1 (en) * | 2015-09-20 | 2017-03-23 | Dh Technologies Development Pte. Ltd. | Small sample injection vial |
-
2018
- 2018-01-31 CN CN201810098856.5A patent/CN108226264B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5221448A (en) * | 1990-01-25 | 1993-06-22 | Spectra-Physics Analytical, Inc. | Buffer gradient and temperature gradient capillary electrophoresis |
| CN2476817Y (en) * | 2001-04-20 | 2002-02-13 | 天润通微(天津)分析技术有限公司 | Quantitative sample feeding gradient pressured capillary electrophoresis device |
| CN1936568A (en) * | 2005-09-22 | 2007-03-28 | 上海通微分析技术有限公司 | Quantitative high-precision capillary tube electrophoresis apparatus |
| CN201540271U (en) * | 2009-06-18 | 2010-08-04 | 广东科贸职业学院 | Capillary electrophoresis electrokinetic injection device |
| CN104395748A (en) * | 2012-06-26 | 2015-03-04 | 安捷伦科技有限公司 | Fluidic valve with selectively switchable storage paths |
| CN105723212A (en) * | 2013-07-08 | 2016-06-29 | 株式会社日立高新技术 | Capillary electrophoresis device |
| CN204989098U (en) * | 2015-08-03 | 2016-01-20 | 李绍平 | Capillary electrophoresis and electrochromatography plug -in components |
| WO2017046783A1 (en) * | 2015-09-20 | 2017-03-23 | Dh Technologies Development Pte. Ltd. | Small sample injection vial |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113454451A (en) * | 2019-02-28 | 2021-09-28 | 思拓凡瑞典有限公司 | Control of buffer preparation process |
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