CN201540271U - Capillary electrophoresis electrokinetic injection device - Google Patents
Capillary electrophoresis electrokinetic injection device Download PDFInfo
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- CN201540271U CN201540271U CN2009200587176U CN200920058717U CN201540271U CN 201540271 U CN201540271 U CN 201540271U CN 2009200587176 U CN2009200587176 U CN 2009200587176U CN 200920058717 U CN200920058717 U CN 200920058717U CN 201540271 U CN201540271 U CN 201540271U
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- rifle
- electrokinetic injection
- capillary electrophoresis
- injection device
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Abstract
The utility model relates t a capillary electrophoresis electrokinetic injection device, in particular to a sampling device for realizing the electrokinetic injection of microliter grade fewer samples. The capillary electrophoresis electrokinetic injection device comprises a bracket, location adjustors, a tube electrode, an electrode power supplying clamp, a hammerhead seat, a hammerhead, a hammerhead cover and a capillary, wherein a support arm is connected to the bracket; two location adjustors at the upper side and the lower side are fixedly connected to the support arm; the lower end of the location adjustor at the lower side is connected with the hammerhead seat; the hammerhead is arranged on the hammerhead seat; a muzzle of the hammerhead is covered by the hammerhead cover; the tube electrode passes through the middles of the two location adjustors to reach the muzzle of the hammerhead; the capillary is sheathed in the tube electrode; and the electrode power supplying clamp is sheathed on the tube electrode. The capillary electrophoresis electrokinetic injection device has the advantages of simple structure and convenient use and operation; the electrokinetic injection of 5 to 200 uL of fewer samples can be realized; and the sampling repetitiveness is good.
Description
Technical field
The utility model designs a kind of Capillary Electrophoresis electrokinetic injection device, relates in particular to the sampling device that a kind of micro updating small amount of sample is realized electrokinetic injection.
Background technology
(capillary electrophoresis CE) is high performance capillary electrophoresis (HPCE) again to Capillary Electrophoresis, is one of analytical approach with fastest developing speed in this year.Capillary Electrophoresis is to be split tunnel with the elastic quartz capillary tube, is driving force with the high-voltage dc, relies in the sample difference on the mobility and distribution behavior between each component and the electrophoretic separation analytical approach that realizes separating.The quartz capillary column that Capillary Electrophoresis is used, under the situation of pH value＞3, its inner surface belt negative electricity, form electrostatic double layer when contacting with damping fluid, under the high-voltage electric field effect, the damping fluid that forms electrostatic double layer one side moves to the negative pole direction owing to positively charged, thereby forms electroosmotic flow.Simultaneously, in buffer solution, charged particle moves to its electrically charged polarity reverse direction with friction speed separately under electric field action, forms electrophoresis.The migration velocity of charged particle in the kapillary damping fluid equal electrophoresis and electroosmotic flow vector and.Various particles are realized separation owing to the equal factor of electrically charged how much, the quality of institute, volume and shape causes that migration velocity is different.
Capillary Electrophoresis has been widely used in the quantitative test in fields such as food, medicine, environment now.The sample injection method of Capillary Electrophoresis mainly contains two kinds, and a kind of is pressure sample introduction (comprising the siphon sample introduction), and a kind of is electrokinetic injection.Wherein the electrokinetic injection technology can be utilized an enlarge-effect easily, and sample is carried out enrichment, and a lot of to the electrokinetic injection Study on Technology in recent years, especially electrical field magnified injection and selectivity exhaust sampling technique.But, present commercial apparatus does not generally design supporting professional electrokinetic injection device, researchers generally are to use the container of 0.5-20mL or the supporting reservoir of capillary electrophoresis apparatus to contain sample, directly be installed on the separate mesh electrode, apply voltage, electrokinetic injection, this electrokinetic injection mode, the first, amount of samples is big, and is for sample preciousness or the few situation of sample size, very inapplicable; The second, the relative position of electrode and capillary exit has greatly changed between twice sample introduction, and sample introduction is poor repeatability as a result; The 3rd, owing to when using selectivity to exhaust sample introduction sample is directly carried out qualitative analysis, require the inorganic ions total amount in the sample few, so used sample size should less (as tens microlitres) during sample introduction, and general electrokinetic injection mode can not be suitable for.
Summary of the invention
In order to overcome above-mentioned defective, the utility model provides a kind of Capillary Electrophoresis electrokinetic injection device that can realize micro updating sample electrokinetic injection.
Capillary Electrophoresis electrokinetic injection device of the present utility model, comprise support, positioning regulator, hollow edged electrode, the power supply folder, the rifle headstock, the rifle head, rifle skull and kapillary, be connected with support arm on the described support, fixedly connected two positioning regulators up and down on the described support arm, the lower end of the positioning regulator of described lower end is connected with the rifle headstock, described rifle head is arranged on the rifle headstock, described rifle skull is sealed the muzzle of described rifle head, the middle part that described hollow edged electrode passes two positioning regulators arrives in the rifle head, in the rifle head sample is housed, hollow edged electrode arrives the sample solution liquid level position of about 1mm down, described kapillary is set in the described hollow edged electrode, and described electrode power supply chuck is located on the described hollow edged electrode.
Further, when sample solution is less than 10uL, then supplemantary electrode need be installed, described supplemantary electrode is arranged on the interior hollow edged electrode position of described rifle head.
The low side of described rifle skull is provided with a rubber cushion, and described rubber cushion is sealed the mouth of robbing of rifle head.Preferably, described rifle skull is provided with clean window near the seat of rubber cushion, the usefulness of cleaning rubber cushion during for the replacing sample.More preferably, described rifle skull is provided with scale, does reference by location during for installing electrodes and kapillary.
Described positioning regulator is provided with the location and regulates nut.By two positioning regulators up and down, can regulate the position of hollow edged electrode easily.
During use, earlier the liquid-transfering gun head is installed to liquid-transfering gun, pipettes the sample solution of a certain amount of (as 20uL), entangle the rifle head with the rifle skull, seal muzzle, take out the liquid-transfering gun head again, be installed on the rifle headstock, when installing for the first time, regulate the positioning of electrode regulator, hollow edged electrode is on the axis of liquid-transfering gun head, and make electrode insert the about 1mm of sample solution, when sample solution is less than 10uL, then supplemantary electrode need be installed.Then kapillary is passed hollow edged electrode gently, be inserted into the about 2mm of rubber cushion place from rifle skull bottom.At last, install the electrode power supply folder, can begin electrokinetic injection.
Capillary Electrophoresis electrokinetic injection device of the present utility model, simple in structure, use easy to operately, can realize electrokinetic injection to the small amount of sample of 5-200uL, the sample introduction good reproducibility is applicable to that selectivity exhausts the not treated direct injected qualitative analysis of sample in the sample introduction.
Introduce Capillary Electrophoresis electrokinetic injection device of the present utility model in detail below in conjunction with accompanying drawing.
Description of drawings
Fig. 1 is a Capillary Electrophoresis electrokinetic injection apparatus structure synoptic diagram of the present utility model;
Fig. 2 is the vertical view of positioning regulator;
Fig. 3 is the structural representation of rifle skull.
Embodiment
As shown in Figure 1, Capillary Electrophoresis electrokinetic injection device of the present utility model, comprise support 9, positioning regulator 3,31, hollow edged electrode 2, power supply folder 4, rifle headstock 5, rifle head 6, rifle skull 7 and kapillary 1, be connected with support arm 91 on the described support 9, fixedly connected two positioning regulators 3 up and down on the described support arm 91,31, the lower end of the positioning regulator 31 of described lower end is connected with rifle headstock 5, described rifle head 6 is arranged on the rifle headstock 5, described rifle skull 7 is sealed the muzzle of described rifle head 6, be placed into the position of the about 1mm of sample solution liquid level in the rifle head, described hollow edged electrode 2 passes two positioning regulators 3,31 middle part arrives mouthful position of robbing of rifle head 6, described kapillary 1 is set in the described hollow edged electrode 2, and described electrode power supply folder 4 is set on the described hollow edged electrode 2.
When sample solution is less than 10uL, then supplemantary electrode 8 need be installed, described supplemantary electrode is arranged on the hollow edged electrode 2 in the described rifle head 6.
As schematically shown in Figure 3, the low side of described rifle skull 7 is provided with a rubber cushion 14, and described rubber cushion is sealed the mouth of robbing of rifle head 6.In a preferred embodiment of the present utility model, described rifle skull 7 is provided with clean window 13 near the seat of rubber cushion 17, the usefulness of cleaning rubber cushion 14 during for the replacing sample.In another preferred embodiment of the present utility model, described rifle skull is provided with scale 12, does reference by location during for installing electrodes and kapillary.
As shown in Figure 2, described positioning regulator 3 and 31 is provided with three location and regulates nut 11.By two positioning regulators 3 and 31 up and down, can make things convenient for, regulate accurately the position of hollow edged electrode, hollow edged electrode is on the axis of rifle head, during use, generally regulate hollow edged electrode, the liquid level that is inserted into the sample in the rifle head is the position of about 1mm down.The relative position that kapillary is inserted in the rifle head also can be regulated by positioning regulator, and kapillary generally is inserted into the position from the about 1mm of muzzle.
The above only is preferred embodiment of the present utility model; not in order to restriction the utility model; all within spirit of the present utility model and principle, any modification of being done, be equal to replacement, improvement etc., all should be included within the protection domain of the present utility model.
Claims (6)
1. Capillary Electrophoresis electrokinetic injection device, it is characterized in that, comprise support, positioning regulator, hollow edged electrode, the power supply folder, the rifle headstock, the rifle head, rifle skull and kapillary, be connected with support arm on the described support, fixedly connected two positioning regulators up and down on the described support arm, the lower end of the positioning regulator of described lower end is connected with the rifle headstock, described rifle head is arranged on the rifle headstock, described rifle skull is sealed the muzzle of described rifle head, the middle part that described hollow edged electrode passes two positioning regulators arrives rifle head inside, described kapillary is set in the described hollow edged electrode, and described electrode power supply folder is provided with and is set on the described hollow edged electrode.
2. Capillary Electrophoresis electrokinetic injection device as claimed in claim 1 is characterized in that the hollow edged electrode position in the described rifle head is provided with supplemantary electrode.
3. Capillary Electrophoresis electrokinetic injection device as claimed in claim 1 or 2 is characterized in that the low side of described rifle skull is provided with a rubber cushion, and described rubber cushion is sealed the mouth of robbing of rifle head.
4. Capillary Electrophoresis electrokinetic injection device as claimed in claim 3 is characterized in that, described positioning regulator is provided with the location and regulates nut.
5. Capillary Electrophoresis electrokinetic injection device as claimed in claim 4 is characterized in that, described rifle skull is provided with clean window near the seat of rubber cushion.
6. Capillary Electrophoresis electrokinetic injection device as claimed in claim 5 is characterized in that described rifle skull is provided with scale.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200587176U CN201540271U (en) | 2009-06-18 | 2009-06-18 | Capillary electrophoresis electrokinetic injection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200587176U CN201540271U (en) | 2009-06-18 | 2009-06-18 | Capillary electrophoresis electrokinetic injection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201540271U true CN201540271U (en) | 2010-08-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009200587176U Expired - Fee Related CN201540271U (en) | 2009-06-18 | 2009-06-18 | Capillary electrophoresis electrokinetic injection device |
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| CN (1) | CN201540271U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226264A (en) * | 2018-01-31 | 2018-06-29 | 重庆医药高等专科学校 | A kind of dribble dropping buffer solution gradient operating system and its operating method |
| CN112993760A (en) * | 2021-02-08 | 2021-06-18 | 桂林雷光科技有限公司 | Semiconductor manufacturing method |
-
2009
- 2009-06-18 CN CN2009200587176U patent/CN201540271U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108226264A (en) * | 2018-01-31 | 2018-06-29 | 重庆医药高等专科学校 | A kind of dribble dropping buffer solution gradient operating system and its operating method |
| CN108226264B (en) * | 2018-01-31 | 2020-11-03 | 重庆医药高等专科学校 | Dropping type buffer solution gradient operation system and operation method thereof |
| CN112993760A (en) * | 2021-02-08 | 2021-06-18 | 桂林雷光科技有限公司 | Semiconductor manufacturing method |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100804 Termination date: 20110618 |