CN104535780A - Micro-fluidic chip for fixing particles, sensor and particle fixing method of micro-fluidic chip - Google Patents
Micro-fluidic chip for fixing particles, sensor and particle fixing method of micro-fluidic chip Download PDFInfo
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- CN104535780A CN104535780A CN201510012695.XA CN201510012695A CN104535780A CN 104535780 A CN104535780 A CN 104535780A CN 201510012695 A CN201510012695 A CN 201510012695A CN 104535780 A CN104535780 A CN 104535780A
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Abstract
The invention relates to the field of detection of particles in gas or liquid, in particular to a micro-fluidic chip for fixing particles in gas or liquid, a sensor and a particle fixing method of the micro-fluidic chip. The micro-fluidic chip for fixing the particles comprises a substrate and a flexible or elastic suspension membrane, and is characterized in that the substrate is provided with a groove structure, the groove structure of the substrate and the flexible or elastic suspension membrane form a sample channel, and the flexible or elastic suspension membrane can deform under the action of external force. The micro-fluidic chip for fixing the particles has the advantages that the flexible or elastic suspension membrane can cover multiple particles at the same time and be fixed to the surface of the sample channel to achieve batched fixing, so that the efficiency of detection and analysis is improved.
Description
Technical field
The present invention relates to the detection field of the particulate in gas or liquid, in particular to a kind of micro-fluidic chip, sensor and particulate fixing means thereof for fixing particulate in gas or liquid, to can be applicable in haze in air, blood the detection field such as microorganism in cell and water.
Background technology
The fusion of optical detective technology and micro-fluidic chip, has expedited the emergence of " optofluidic " (optofluidic) sensor (Nature Photon., 5 (10), 591-597 (2011)).Utilize optofluidic sensor to carry out analysis to gas or fluid sample to detect, there is high sensitivity, in real time dynamically and the advantage such as micro-example demand.Therefore; how to utilize optofluidic sensor detection of particles thing; in the field such as environmental protection and biochemical analysis, there is important application; such as: the particulate suspended in air can cause haze to pollute; cell in blood can reflect health status, and in seawater, nanoplankton can affect water quality (Biogeosciences, 7; 3239-3257,2010).High-precision optical detection, needs particulate to fix usually.But small-sized (can be as small as several nanometer) of particulate not easily fixes, and how to fix particulate, particularly how quick, large batch of fixing particulate, realization is analyzed efficiently, accurately to detection most important.
In prior art, patent CN200680049247.6 disclose particulate particularly biological particles characterize and counting method and apparatus, by particulate, the principle that may be present on the stable equilibrium point (PEQ) acting on the field of force (F) on described particulate produced by the array of electrode (EL) realizes, the field of force can be dielectrophoresis that is positive or that bear, electrophoresis or electrohydrodynamics campaign, it is characterized in that one group of stable equilibrium point about particulate (solid-state, liquid or gaseous state); Be called that by utilizing international scientific circle dielectric powers on wetting effect, same method is applicable to handles droplet (liquid particulate).But its textural anomaly is complicated, and the manufacturing cost of described device will be extremely expensive.
But particulate can not be fixed thus reach accurate detection by common micro-fluidic chip, therefore, how in micro-fluidic chip, fixing large batch of particulate rapidly and efficiently, thus provide a kind of low cost of manufacture, structure simple, can large batch of fixing particulate, realizing micro-fluidic chip that is efficient, accurately detection of particles, is that wound of the present invention grinds motivation.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, there is provided a kind of cheap for manufacturing cost, structure is simple, required sample is few, can large batch of fixing particulate, realize efficient, accurately to detect fixing particulate micro-fluidic chip, sensor and particulate fixing means thereof.
The micro-fluidic chip of a kind of fixing particulate provided by the present invention, its main technical schemes is as follows:
A micro-fluidic chip for fixing particulate, comprise substrate, flexible outstanding film or elastic suspended film, it is characterized in that: described substrate has groove structure, the groove structure of substrate and described flexibility hang film or described elastic suspended film forms sample channel; Described flexibility hangs film or described elastic suspended film can be out of shape under the effect of external force.
The micro-fluidic chip of a kind of fixing particulate provided by the present invention, also can comprise following attached technical scheme:
Wherein, described sample channel surface etch has micro-hole.
Wherein, the shape in described micro-hole is arranged as required, for the one in point-like, line style, circle, polygon or appoint several.
Wherein, described micro-fluidic chip is provided with upstream and downstream pipe.
Wherein, described upstream is provided with upstream valve.
Wherein, described downstream pipe is provided with draw-out device and/or valve downstream.
Wherein, described external force be in liquid capillary force, pressure, fluid draft any one or appoint several.
Wherein, described backing material is selected from the one in glass material, organic material or crystalline material.
Present invention also offers a kind of sensor of the micro-fluidic chip containing fixing particulate, its main technical schemes is:
A kind of sensor of the micro-fluidic chip containing fixing particulate, comprise: peripheral sampling system, micro-fluidic chip, collecting sensor signal and disposal system and platform assembly, is characterized in that: described micro-fluidic chip is arbitrary micro-fluidic chip described above.
Present invention also offers the particulate fixing means in a kind of micro-fluidic chip, it is characterized in that, mainly comprise the following steps:
The first step, by the gas containing particulate to be measured or liquid, hang via the groove structure of substrate and flexibility the micro-fluidic chip sample channel that film or elastic suspended film form at the flows by action of external force, discharge finally by downstream pipe;
Second step, all or part of closedown upstream valve, the downstream pipe of sample channel continues withdrawn fluid, pressure durations in sample channel is reduced, thus cause flexible outstanding film or elastic suspended film to be out of shape, to be attached to sample channel surface, particulate covers and is fixed on sample channel surface, for detection of particulates by the flexible outstanding film of realization or elastic suspended film;
After 3rd step, detection of particulates terminate, open upstream valve, refill fluid, flexible outstanding film or elastic suspended film leave sample channel surface, and the particulate being fixed on sample channel surface is taken away by fluid, changes particulate samples.
The present invention hangs film or elastic suspended film by the groove structure surface suspension one deck flexibility at the substrate of micro-fluidic chip, after fluid upstream pipeline is closed, under the effect of the draft of downstream line and the capillary force of fluid, flexible outstanding film or elastic suspended film is made to be out of shape and to be attached to sample channel surface, thus the particulate in fluid is covered below flexible outstanding film or elastic suspended film, to realize fixing a large amount of particulate of quickness and high efficiency.Further, by the micro-hole of sample channel surface etch, particulate is preferentially fixed in micro-hole, thus the particulate realizing position controlled is fixed.
As shown in Figure 1, be above the groove of chip, fix the outstanding film of one deck flexibility or elastic suspended film, flexible outstanding film or elastic suspended film form the fluid sample passage of sealing to microfluidic chip structure of the present invention together with substrate.
The principle of work of described particulate fixing means is (as Fig. 2): containing fine-grained fluid, under the effect of downstream draft, through upstream valve, flow through there is outstanding film sample channel, discharge finally by downstream pipe; After upstream valve is closed, the draft in downstream continues withdrawn fluid, pressure durations in sample channel is reduced, thus causes flexible outstanding film or elastic suspended film are out of shape and are attached to sample channel surface, realize particulate to cover the object being fixed on sample channel surface.
Described flexibility hangs film or elastic suspended film, can be covered by multiple particulate simultaneously and be fixed on sample channel surface, realizes batch fixing, thus improves the efficiency detecting and analyze.
Described particulate fixing means, after upstream valve reopens, because fluid refills sample channel, make flexible outstanding film or elastic suspended film recover unsettled state, the particulate be fixed flows away thereupon, thus realizes the object changing detection of particles sample.Therefore, without the need to complex operations, just testing sample can be changed.
Described fluid, air inclusion and liquid.
Described fluid sample passage, can prepare small pit (being called for short " micro-hole ") (as Fig. 3) on sample channel surface.Be out of shape when outstanding film and be attached in the process on sample channel surface, particulate is preferentially fixing in micro-hole, thus the fixed position realizing particulate is controlled, is conducive to the array arrangement forming particulate, is easy to Aulomatizeted Detect in enormous quantities.
Described particulate fixing means, after upstream valve is closed, along with liquid in sample channel reduces, the capillary force of liquid can promote that outstanding film is out of shape and is attached to sample channel surface.
Described particulate fixing means, upstream valve Close All or part are closed, and outstanding film all can be impelled to be out of shape and to be attached to sample channel surface.
Accompanying drawing explanation
Accompanying drawing, it is incorporated into and becomes the part of this instructions, demonstrates embodiments of the invention, and explains principle of the present invention with aforesaid summary together with detailed description below.
Fig. 1 is the microfluidic chip structure schematic diagram for fixing particulate.
Fig. 2 is particulate fixing means schematic diagram in micro-fluidic chip.
Fig. 3 is the controlled particulate fixing means schematic diagram in position.
1, substrate; 2, flexible outstanding film or elastic suspended film; 3, sample channel; 4, upstream valve; 5, particulate; 6, Wei Keng; 7, upstream; 8, downstream pipe.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the present invention is described in detail, it is pointed out that described embodiment is only intended to be convenient to the understanding of the present invention, and any restriction effect is not play to it.
Embodiment 1
As shown in Figure 1, Figure 2 and Figure 3, a kind of micro-fluidic chip of fixing particulate, comprise substrate 1, flexible outstanding film or elastic suspended film 2, it is characterized in that: described substrate 1 has groove structure, the groove structure of substrate 1 and described flexibility hang film or described elastic suspended film forms sample channel 3; Described flexibility hangs film or described elastic suspended film can be out of shape under the effect of external force; Wherein, described sample channel 3 surface etch has micro-hole 6; Particulate 5 is preferentially fixed in micro-hole 6, thus it is fixing to realize the controlled particulate in position 5; The shape in described micro-hole 6 is arranged as required, for the one in point-like, line style, circle, polygon or appoint several; The preferred linear array of the present embodiment, can realize Aulomatizeted Detect; Wherein, described micro-fluidic chip is provided with upstream 7 and downstream pipe 8; Described upstream 7 is provided with upstream valve 4; Described downstream pipe is provided with draw-out device and/or valve downstream; Wherein, described substrate 1 material is selected from the one in glass material, organic material or crystalline material, the present embodiment preferred glass material; Wherein, described external force can be in liquid capillary force, pressure, fluid draft any one or appoint several.
Present invention also offers a kind of sensor of the micro-fluidic chip containing fixing particulate, its main technical schemes is:
A kind of sensor of the micro-fluidic chip containing fixing particulate, comprise: peripheral sampling system, micro-fluidic chip, collecting sensor signal and disposal system and platform assembly, it is characterized in that: described micro-fluidic chip comprises substrate 1, flexible outstanding film or elastic suspended film 2, it is characterized in that: described substrate 1 has groove structure, the groove structure of substrate 1 and described flexibility hang film or described elastic suspended film forms sample channel 3; Described flexibility hangs film or described elastic suspended film can be out of shape under the effect of external force.
In the present invention, a kind of structure of sensor of the micro-fluidic chip containing fixing particulate is conventional structure, and the improvement of sensor provided by the invention only relates to above-mentioned micro-fluidic chip, does not change other parts.Therefore this instructions only describes in detail micro-fluidic chip, the miscellaneous part of sensor and principle of work are repeated no more here.In the content basis that those skilled in the art describe at this instructions, micro-fluidic control chip sensor of the present invention can be realized.
Present invention also offers the particulate fixing means in a kind of micro-fluidic chip, it is characterized in that, mainly comprise the following steps:
The first step, by the gas containing particulate 5 to be measured or liquid, hang via the groove structure of substrate 1 and flexibility the micro-fluidic chip sample channel 3 that film or elastic suspended film 2 form at the flows by action of external force, discharge finally by downstream pipe 8;
Second step, all or part of closedown upstream valve 4, the downstream pipe 8 of sample channel 3 continues withdrawn fluid, pressure durations in sample channel 3 is reduced, thus cause flexible outstanding film or elastic suspended film 2 to be out of shape, to be attached to sample channel 3 surface, particulate 5 covers and is fixed on sample channel 3 surface by the flexible outstanding film of realization or elastic suspended film 2, detects for particulate 5;
3rd step, particulate 5 are opened upstream valve 4, are refilled fluid after detecting and terminating, and flexible outstanding film or elastic suspended film 2 leave sample channel 3 surface, and the particulate 5 being fixed on sample channel 3 surface is taken away by fluid, is convenient for changing particulate 5 sample.
Embodiment 2
First, prepare groove structure on substrate 1, then substrate 1 and flexibility are hanged film bonding, to form the micro-fluidic chip with sealed hollow structure, hollow structure is wherein used as fluid sample passage 3 (as Fig. 1).
Gas containing particulate 5 to be measured or liquid, under the effect of downstream draft, through upstream valve 4, flow through there is outstanding film sample channel 3, discharge finally by downstream pipe 8.
Subsequently, all or part of closedown upstream valve 4, withdrawn fluid is continued in downstream, pressure durations in sample channel 3 is reduced, thus cause flexible outstanding film be out of shape and be attached to sample channel 3 surface, realize particulate 5 to cover and be fixed on sample channel 3 surface and carry out optical detection (as Fig. 2).
After optical detection terminates, open upstream valve 4, refill fluid, outstanding film leaves sample channel 3 surface, and the particulate 5 being fixed on sample channel 3 surface is taken away (as Fig. 1) by fluid.
In sum, particulate fixing means in micro-fluidic chip provided by the invention, by the outstanding film of fluid sample passage 3 surface suspension one deck flexibility at micro-fluidic chip, utilize fluid draft and fluid capillary force, make flexible outstanding film be out of shape and be attached to sample channel 3 surface, fixing a large amount of particulates 5 of quickness and high efficiency can be realized.
Finally should be noted that; above embodiment is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; although done to explain to the present invention with reference to preferred embodiment; those of ordinary skill in the art is to be understood that; can modify to technical scheme of the present invention or equivalent replacement, and not depart from essence and the scope of technical solution of the present invention.
Claims (10)
1. a micro-fluidic chip for fixing particulate, comprise substrate, flexible outstanding film or elastic suspended film, it is characterized in that: described substrate has groove structure, the groove structure of substrate and described flexibility hang film or described elastic suspended film forms sample channel; Described flexibility hangs film or described elastic suspended film can be out of shape under the effect of external force.
2. a micro-fluidic chip for a kind of fixing particulate as claimed in claim 1, is characterized in that: described sample channel surface etch has micro-hole.
3. a micro-fluidic chip for a kind of fixing particulate as claimed in claim 2, is characterized in that: the shape in described micro-hole is arranged as required, for the one in point-like, line style, circle, polygon or appoint several.
4. a micro-fluidic chip for a kind of fixing particulate as claimed in claim 1, is characterized in that: described micro-fluidic chip is provided with upstream and downstream pipe.
5. a micro-fluidic chip for a kind of fixing particulate as claimed in claim 4, is characterized in that: described upstream is provided with upstream valve.
6. a micro-fluidic chip for a kind of fixing particulate as claimed in claim 4, is characterized in that: described downstream pipe is provided with draw-out device and/or valve downstream.
7. a micro-fluidic chip for a kind of fixing particulate as claimed in claim 1, is characterized in that: described external force be in liquid capillary force, pressure, fluid draft any one or appoint several.
8. a micro-fluidic chip for a kind of fixing particulate as claimed in claim 1, is characterized in that: described backing material is selected from the one in glass material, organic material or crystalline material.
9. the sensor of the micro-fluidic chip containing fixing particulate, comprise: peripheral sampling system, micro-fluidic chip, collecting sensor signal and disposal system and platform assembly, is characterized in that: described micro-fluidic chip is the micro-fluidic chip described in any one of claim 1-8.
10. the particulate fixing means in micro-fluidic chip, is characterized in that, mainly comprise the following steps:
The first step, by the gas containing particulate to be measured or liquid, hang via the groove structure of substrate and flexibility the micro-fluidic chip sample channel that film or elastic suspended film form at the flows by action of external force, discharge finally by downstream pipe;
Second step, all or part of closedown upstream valve, the downstream pipe of sample channel continues withdrawn fluid, pressure durations in sample channel is reduced, thus cause flexible outstanding film or elastic suspended film to be out of shape, to be attached to sample channel surface, particulate covers and is fixed on sample channel surface, for detection of particulates by the flexible outstanding film of realization or elastic suspended film;
After 3rd step, detection of particulates terminate, open upstream valve, refill fluid, flexible outstanding film or elastic suspended film leave sample channel surface, and the particulate being fixed on sample channel surface is taken away by fluid, changes particulate samples.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510012695.XA CN104535780B (en) | 2014-11-05 | 2015-01-08 | The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof |
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| CN201410654625 | 2014-11-05 | ||
| CN201410654625X | 2014-11-05 | ||
| CN201510012695.XA CN104535780B (en) | 2014-11-05 | 2015-01-08 | The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof |
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| CN104535780A true CN104535780A (en) | 2015-04-22 |
| CN104535780B CN104535780B (en) | 2016-09-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345935A (en) * | 2016-05-06 | 2017-11-14 | 中国科学院化学研究所 | A kind of method of micro-fluidic reactor and its application and detecting system and detection liquid interface ion distribution |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1987420A (en) * | 2006-12-30 | 2007-06-27 | 清华大学 | Microflow control chip device for multiple function detecting single particle matter |
| CN101290314A (en) * | 2008-03-07 | 2008-10-22 | 重庆大学 | Microfluidic control chip for cell immobilization and solution dilution |
| CN101903105A (en) * | 2007-12-14 | 2010-12-01 | 皇家飞利浦电子股份有限公司 | Microfluidic device and method of making the same and sensor incorporating the same |
| CN102791370A (en) * | 2010-03-17 | 2012-11-21 | 罗伯特·博世有限公司 | Microarray comprising immobilisation particles |
| CN103434060A (en) * | 2013-08-07 | 2013-12-11 | 苏州扬清芯片科技有限公司 | Micro-fluidic chip die |
-
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- 2015-01-08 CN CN201510012695.XA patent/CN104535780B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1987420A (en) * | 2006-12-30 | 2007-06-27 | 清华大学 | Microflow control chip device for multiple function detecting single particle matter |
| CN101903105A (en) * | 2007-12-14 | 2010-12-01 | 皇家飞利浦电子股份有限公司 | Microfluidic device and method of making the same and sensor incorporating the same |
| CN101290314A (en) * | 2008-03-07 | 2008-10-22 | 重庆大学 | Microfluidic control chip for cell immobilization and solution dilution |
| CN102791370A (en) * | 2010-03-17 | 2012-11-21 | 罗伯特·博世有限公司 | Microarray comprising immobilisation particles |
| CN103434060A (en) * | 2013-08-07 | 2013-12-11 | 苏州扬清芯片科技有限公司 | Micro-fluidic chip die |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345935A (en) * | 2016-05-06 | 2017-11-14 | 中国科学院化学研究所 | A kind of method of micro-fluidic reactor and its application and detecting system and detection liquid interface ion distribution |
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