CN104535780B - The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof - Google Patents
The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof Download PDFInfo
- Publication number
- CN104535780B CN104535780B CN201510012695.XA CN201510012695A CN104535780B CN 104535780 B CN104535780 B CN 104535780B CN 201510012695 A CN201510012695 A CN 201510012695A CN 104535780 B CN104535780 B CN 104535780B
- Authority
- CN
- China
- Prior art keywords
- microgranule
- micro
- film
- fluidic chip
- fixing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention relates to the detection field of microgranule in gas or liquid, in gas or liquid, the micro-fluidic chip of microgranule, sensor and microgranule fixing means is fixed particularly to a kind of, a kind of micro-fluidic chip of fixing microgranule, including substrate, flexible outstanding film or elastic suspended film, it is characterized in that: described substrate has groove structure, the groove structure of substrate and described flexibility hang film or described elastic suspended film constitutes sample channel；Described flexibility hangs film or described elastic suspended film can deform under the effect of external force.It provides the benefit that described flexibility hangs film or elastic suspended film, can cover and be fixed on sample channel surface by multiple microgranules simultaneously, it is achieved batch is fixing, thus improves the efficiency that detection is analyzed.
Description
Technical field
The present invention relates to the detection field of microgranule in gas or liquid, particularly to one for gas or liquid
Body is fixed the micro-fluidic chip of microgranule, sensor and microgranule fixing means thereof, can be applicable to haze in air,
The detection field such as microorganism in cell and water in blood.
Background technology
Optical detective technology and the fusion of micro-fluidic chip, expedited the emergence of " optofluidic " (optofluidic) sensor
(Nature Photon., 5 (10), 591-597 (2011)).Utilize optofluidic sensor to gas or fluid sample
It is analyzed detection, there is the advantages such as high sensitivity, Real-time and Dynamic and micro-example demand.Therefore, as
What utilizes optofluidic sensor detection of particles thing, has important application in the field such as environmental conservation and biochemical analysis,
Such as: the microgranule suspended in air can cause haze to pollute, and the cell in blood can reflect health status,
In sea water, nanoplankton can affect water quality (Biogeosciences, 7,3239-3257,2010).In high precision
Optical detection, it usually needs microgranule is fixed.But, the small-sized of microgranule (can be as small as several
Nanometer) it is not fixed easily, how to fix microgranule, the most fast, large batch of fixing microgranule, for
Realize analyzing detection efficiently, accurately most important.
In prior art, patent CN200680049247.6 discloses microgranule particularly biological particles and characterizes
With the method and apparatus of counting, it is likely to be present in by microgranule and is acted on institute by what the array of electrode (EL) produced
The principle stated on the stable equilibrium point (PEQ) of the field of force on microgranule (F) realizes, and the field of force can be positive or negative
Dielectrophoresis, electrophoresis or Electrofluid Mechanics campaign, it is characterised in that relevant microgranule (solid-state, liquid or gas
State) one group of stable equilibrium point；By utilizing international scientific circle to be referred to as the effect of electrowetting on electrolyte, equally
Method be applicable to handle droplet (liquid particulate).But its textural anomaly is complicated, the manufacture of described device
Cost will be the most expensive.
But commonly microgranule can not be fixed thus reach accurately to detect, therefore, how micro-by micro-fluidic chip
In fluidic chip, fixing large batch of microgranule rapidly and efficiently, thus a kind of low cost of manufacture, structure are provided
Simply, it is possible to large batch of fixing microgranule, it is achieved the micro-fluidic chip of detection of particles efficient, accurate, it is this
The wound of invention grinds motivation.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that one is cheap for manufacturing cost, structure is simple
List, required sample are few, it is possible to large batch of fixing microgranule, it is achieved efficiently, accurately the fixing microgranule of detection is micro-
Fluidic chip, sensor and microgranule fixing means thereof.
The micro-fluidic chip of a kind of fixing microgranule provided by the present invention, its main technical schemes is as follows:
The micro-fluidic chip of a kind of fixing microgranule, including substrate, flexible outstanding film or elastic suspended film, its feature
Being: described substrate has groove structure, the groove structure of substrate and described flexibility hang film or described elasticity
Outstanding film constitutes sample channel；Described flexibility hangs film or described elastic suspended film can deform under the effect of external force.
The micro-fluidic chip of a kind of fixing microgranule provided by the present invention, may also include following attached technical scheme:
Wherein, described sample channel surface etch has micro-hole.
Wherein, the shape in described micro-hole is arranged as required to, in point-like, line style, circle, polygon
A kind of or appoint several.
Wherein, described micro-fluidic chip is provided with upstream and downstream pipe.
Wherein, described upstream is provided with upstream valve.
Wherein, described downstream pipe is provided with draw-out device and/or valve downstream.
Wherein, described external force is any one in liquid capillary force, pressure, fluid draft or appoints several.
Wherein, described backing material one in glass material, organic material or crystalline material.
Present invention also offers the sensor of a kind of micro-fluidic chip containing fixing microgranule, its major technique side
Case is:
The sensor of a kind of micro-fluidic chip containing fixing microgranule, including: peripheral sampling system, micro-fluidic
Chip, collecting sensor signal and processing system and platform assembly, it is characterised in that: described miniflow
Control chip is arbitrary micro-fluidic chip described above.
Present invention also offers the microgranule fixing means in a kind of micro-fluidic chip, it is characterised in that mainly wrap
Include following steps:
The first step, by the gas containing microgranule to be measured or liquid, dirty recessed via substrate of effect in external force
The micro-fluidic chip sample channel that groove structure and flexibility hang film or elastic suspended film is constituted, after through downstream pipe
Discharge；
Second step, completely or partially closing upstream valve, the downstream pipe of sample channel continues withdrawn fluid,
Pressure in sample channel is persistently reduced, thus causes flexible outstanding film or the deformation of elastic suspended film, attach
On sample channel surface, it is achieved microgranule is covered and is fixed on sample channel table by flexible outstanding film or elastic suspended film
Face, for detection of particulates；
After 3rd step, detection of particulates terminate, open upstream valve, refill fluid, flexible outstanding film or
Person's elastic suspended film leaves sample channel surface, and the microgranule being fixed on sample channel surface is carried away by the flow, and changes
Particulate samples.
The present invention is by hanging film or bullet in the groove structure surface of the substrate of micro-fluidic chip one layer of flexibility of suspension
Property hangs film, after fluid upstream pipeline is closed, at the draft of downstream line and the work of the capillary force of fluid
Under with so that flexible outstanding film or elastic suspended film deform and be attached to sample channel surface, thus by fluid
In microgranule cover hang below film or elastic suspended film in flexibility, to realize fixing a large amount of microgranules of quickness and high efficiency.
Further, by the micro-hole of sample channel surface etch so that microgranule is preferentially fixed in micro-hole, thus realizes
The controlled microgranule in position is fixed.
The microfluidic chip structure of the present invention is as it is shown in figure 1, be the groove one layer of flexibility fixed above at chip
Outstanding film or elastic suspended film, flexible outstanding film or elastic suspended film constitute the fluid sample of sealing together with substrate and lead to
Road.
The operation principle of described microgranule fixing means is (such as Fig. 2): containing fine-grained fluid, take out in downstream
Under the effect of power, through upstream valve, flow through have outstanding film sample channel, after discharge through downstream pipe；
After upstream valve is closed, the draft in downstream continues withdrawn fluid so that the pressure in sample channel continues
Reduce, thus cause flexible outstanding film or elastic suspended film deform and are attached to sample channel surface, it is achieved will
Microgranule covers the purpose being fixed on sample channel surface.
Described flexibility hangs film or elastic suspended film, can cover and be fixed on sample by multiple microgranules simultaneously lead to
Surface, road, it is achieved batch is fixing, thus improve the efficiency that detection is analyzed.
Described microgranule fixing means, after upstream valve reopens, owing to fluid refills sample
Passage so that flexible outstanding film or elastic suspended film recover unsettled state, and the microgranule fixed flows away therewith,
Thus realize changing the purpose of detection of particles sample.It is therefore not necessary to complex operations, it is possible to change and treat test sample
Product.
Described fluid, comprises gas and liquid.
Described fluid sample passage, can prepare small pit (being called for short in " micro-hole ") on sample channel surface
(such as Fig. 3).During outstanding film deforms and is attached to sample channel surface, microgranule is preferentially solid in micro-hole
Fixed, thus the fixed position realizing microgranule is controlled, advantageously forms the array arrangement of microgranule, it is easy in high volume
Aulomatizeted Detect.
Described microgranule fixing means, after upstream valve is closed, along with in sample channel, liquid reduces, liquid
The capillary force of body can promote outstanding film deformation and be attached to sample channel surface.
Described microgranule fixing means, upstream valve is all closed or partially closed, all can promote outstanding film deformation
And it is attached to sample channel surface.
Accompanying drawing explanation
Accompanying drawing, it is incorporated into and becomes the part of this specification, demonstrating embodiments of the invention, and
The principle of the present invention is explained together with aforesaid summary and detailed description below.
Fig. 1 is the microfluidic chip structure schematic diagram for fixing microgranule.
Fig. 2 is microgranule fixing means schematic diagram in micro-fluidic chip.
Fig. 3 is the microgranule fixing means schematic diagram that position is controlled.
1, substrate；2, flexible outstanding film or elastic suspended film；3, sample channel；4, upstream valve；5, micro-
Grain；6, Wei Keng；7, upstream；8, downstream pipe.
Detailed description of the invention
Below in conjunction with embodiment and accompanying drawing, the present invention is described in detail, it should be pointed out that retouched
The embodiment stated is intended merely to facilitate the understanding of the present invention, and it does not play any restriction effect.
Embodiment 1
As shown in Figure 1, Figure 2 and Figure 3, the micro-fluidic chip of a kind of fixing microgranule, including substrate 1, flexibility
Outstanding film or elastic suspended film 2, it is characterised in that: described substrate 1 has groove structure, the groove of substrate 1
Structure and described flexibility hang film or described elastic suspended film constitutes sample channel 3；Described flexibility hangs film or institute
State elastic suspended film can deform under the effect of external force；Wherein, described sample channel 3 surface etch has micro-hole 6；
Microgranule 5 is preferentially fixed in micro-hole 6, thus realizes the controlled microgranule in position 5 and fix；Described micro-hole
The shape of 6 is arranged as required to, for the one in point-like, line style, circle, polygon or appoint several；This
The preferred linear array of embodiment, can realize Aulomatizeted Detect；Wherein, described micro-fluidic chip is provided with upstream
Pipeline 7 and downstream pipe 8；Described upstream 7 is provided with upstream valve 4；Described downstream pipe is provided with
Draw-out device and/or valve downstream；Wherein, described substrate 1 material is selected from glass material, organic material or crystalline substance
One in body material, the preferred glass material of the present embodiment；Wherein, described external force can be liquid capillary
In power, pressure, fluid draft any one or appoint several.
Present invention also offers the sensor of a kind of micro-fluidic chip containing fixing microgranule, its major technique side
Case is:
The sensor of a kind of micro-fluidic chip containing fixing microgranule, including: peripheral sampling system, micro-fluidic
Chip, collecting sensor signal and processing system and platform assembly, it is characterised in that: described miniflow
Control chip includes substrate 1, flexible outstanding film or elastic suspended film 2, it is characterised in that: described substrate 1 has recessed
Groove structure, the groove structure of substrate 1 and described flexibility hang film or described elastic suspended film constitutes sample channel 3；
Described flexibility hangs film or described elastic suspended film can deform under the effect of external force.
In the present invention, the structure of the sensor of a kind of micro-fluidic chip containing fixing microgranule is conventional structure, and
The improvement of the sensor that the present invention provides only relates to above-mentioned micro-fluidic chip, is not modified other parts.
Therefore micro-fluidic chip is only described in detail by this specification, to the miscellaneous part of sensor and operation principle the most not
Repeat again.Those skilled in the art, in the content basis that this specification describes, can realize of the present invention
Micro-fluidic control chip sensor.
Present invention also offers the microgranule fixing means in a kind of micro-fluidic chip, it is characterised in that mainly wrap
Include following steps:
The first step, by the gas containing microgranule 5 to be measured or liquid, the effect in external force is dirty via substrate 1
The micro-fluidic chip sample channel 3 that groove structure and flexibility hang film or elastic suspended film 2 is constituted, after warp
Downstream pipe 8 is discharged；
Second step, all or part of closedown upstream valve 4, the downstream pipe 8 of sample channel 3 continues extraction
Fluid so that the pressure in sample channel 3 persistently reduces, thus cause flexible outstanding film or elastic suspended film 2
Deform, be attached to sample channel 3 surface, it is achieved microgranule 5 is covered also by flexible outstanding film or elastic suspended film 2
It is fixed on sample channel 3 surface, detects for microgranule 5；
After the detection of 3rd step, microgranule 5 terminates, open upstream valve 4, refill fluid, flexible outstanding
Film or elastic suspended film 2 leave sample channel 3 surface, and the microgranule 5 being fixed on sample channel 3 surface is flowed
Body is taken away, and is convenient for changing microgranule 5 sample.
Embodiment 2
First, prepare groove structure on substrate 1, then substrate 1 and flexibility are hanged film bonding, to constitute
Having the micro-fluidic chip sealing hollow structure, hollow structure therein is used as fluid sample passage 3 (such as figure
1)。
Gas containing microgranule 5 to be measured or liquid, under the effect of downstream draft, through upstream valve 4,
Flow through have outstanding film sample channel 3, after discharge through downstream pipe 8.
Subsequently, all or part of upstream valve 4 of closing, downstream continuation withdrawn fluid so that sample channel 3
Interior pressure persistently reduces, thus causes flexible outstanding film deform and be attached to sample channel 3 surface, it is achieved
Microgranule 5 is covered and is fixed on sample channel 3 surface and carries out optical detection (such as Fig. 2).
After optical detection terminates, opening upstream valve 4, refill fluid, outstanding film leaves sample channel 3
Surface, the microgranule 5 being fixed on sample channel 3 surface is carried away by the flow (such as Fig. 1).
In sum, the microgranule fixing means in the micro-fluidic chip that the present invention provides, by micro-fluidic core
Fluid sample passage 3 surface one layer of flexibility of suspension of sheet hangs film, utilizes fluid draft and fluid capillary force, makes
Obtain flexible outstanding film deform and be attached to sample channel 3 surface, it is possible to achieve fixing a large amount of microgranules of quickness and high efficiency
5。
Last it should be noted that, above example is only in order to illustrate technical scheme, rather than to this
The restriction of invention protection domain, although having made to explain to the present invention with reference to preferred embodiment, this area
It is to be appreciated by one skilled in the art that technical scheme can be modified or equivalent,
Spirit and scope without deviating from technical solution of the present invention.
Claims (10)
1. a micro-fluidic chip for fixing microgranule, including substrate, flexible outstanding film or elastic suspended
Film, it is characterised in that: described substrate has groove structure, and the groove structure of substrate hangs with described flexibility
Film or described elastic suspended film constitute sample channel；Described flexibility hangs film or described elastic suspended film outside
Can deform under the effect of power, the described flexibility after deformation hangs film or described elastic suspended film is attached to sample
Channel surface, covers microgranule and is fixed on sample channel surface.
2. a micro-fluidic chip for a kind of fixing microgranule as claimed in claim 1, its feature exists
In: described sample channel surface etch has micro-hole.
3. a micro-fluidic chip for a kind of fixing microgranule as claimed in claim 2, its feature exists
In: the shape in described micro-hole is arranged as required to as the one in point-like, line style, circle, polygon
Or appoint several.
4. a micro-fluidic chip for a kind of fixing microgranule as claimed in claim 1, its feature exists
In: described micro-fluidic chip is provided with upstream and downstream pipe.
5. a micro-fluidic chip for a kind of fixing microgranule as claimed in claim 4, its feature exists
In: described upstream is provided with upstream valve.
6. a micro-fluidic chip for a kind of fixing microgranule as claimed in claim 4, its feature exists
In: described downstream pipe is provided with draw-out device and/or valve downstream.
7. a micro-fluidic chip for a kind of fixing microgranule as claimed in claim 1, its feature exists
In: described external force is any one in liquid capillary force, pressure, fluid draft or appoints several.
8. a micro-fluidic chip for a kind of fixing microgranule as claimed in claim 1, its feature exists
In: described backing material one in glass material, organic material or crystalline material.
9. a sensor for the micro-fluidic chip containing fixing microgranule, including: peripheral sample introduction system
System, micro-fluidic chip, collecting sensor signal and processing system and platform assembly, its feature
It is: described micro-fluidic chip is the micro-fluidic chip described in any one of claim 1-8.
10. the microgranule fixing means in a micro-fluidic chip, it is characterised in that mainly include following
Step:
The first step, by the gas containing microgranule to be measured or liquid, the effect in external force is dirty via substrate
The micro-fluidic chip sample channel that groove structure and flexibility hang film or elastic suspended film is constituted, after warp
Downstream pipe is discharged；
Second step, completely or partially closing upstream valve, the downstream pipe of sample channel continues extraction stream
Body, the power of withdrawn fluid is any one in liquid capillary force, pressure, fluid draft or appoints several,
Pressure in sample channel is persistently reduced, thus cause flexible outstanding film or the deformation of elastic suspended film,
It is attached to sample channel surface, it is achieved microgranule is covered and is fixed on sample by flexible outstanding film or elastic suspended film
Product channel surface, for detection of particulates；
After 3rd step, detection of particulates terminate, open upstream valve, refill fluid, flexible
Outstanding film or elastic suspended film leave sample channel surface, are fixed on the microgranule on sample channel surface by fluid
Take away, change particulate samples.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510012695.XA CN104535780B (en) | 2014-11-05 | 2015-01-08 | The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410654625X | 2014-11-05 | ||
| CN201410654625 | 2014-11-05 | ||
| CN201510012695.XA CN104535780B (en) | 2014-11-05 | 2015-01-08 | The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104535780A CN104535780A (en) | 2015-04-22 |
| CN104535780B true CN104535780B (en) | 2016-09-14 |
Family
ID=52851345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510012695.XA Active CN104535780B (en) | 2014-11-05 | 2015-01-08 | The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104535780B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107345935A (en) * | 2016-05-06 | 2017-11-14 | 中国科学院化学研究所 | A kind of method of micro-fluidic reactor and its application and detecting system and detection liquid interface ion distribution |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100552422C (en) * | 2006-12-30 | 2009-10-21 | 清华大学 | Microfluidic chip device for multifunctional detection of single particulate matter |
| EP2070594A1 (en) * | 2007-12-14 | 2009-06-17 | Koninklijke Philips Electronics N.V. | Microfluidic device and method of making the same and sensor incorporating the same |
| CN101290314A (en) * | 2008-03-07 | 2008-10-22 | 重庆大学 | Microfluidic control chip for cell immobilization and solution dilution |
| DE102010002957A1 (en) * | 2010-03-17 | 2011-09-22 | Robert Bosch Gmbh | Microarray with immobilization particles |
| CN103434060A (en) * | 2013-08-07 | 2013-12-11 | 苏州扬清芯片科技有限公司 | Micro-fluidic chip die |
-
2015
- 2015-01-08 CN CN201510012695.XA patent/CN104535780B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104535780A (en) | 2015-04-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101750450B (en) | Automatic sampling device for array capillary electrophoresis | |
| JP2012513773A5 (en) | ||
| US20200070167A1 (en) | Processing systems for isolating and enumerating cells or particles | |
| DE602005020742D1 (en) | SEQUENCE AMPLIFICATION AND DETECTION METHOD | |
| CN106999928A (en) | Nucleic acid purification box | |
| JP2009503553A5 (en) | ||
| CN202710481U (en) | Device for identifying and measuring air bubble in high-concentration dispersed multiphase flow | |
| CN102628870B (en) | Micro-nanofluidic chip and method for achieving rapid fluorescent labeling of proteins | |
| CN104076023A (en) | Body fluid Raman spectrum testing device | |
| FR2942041B1 (en) | ONBOARD DEVICE FOR ANALYZING A BODILY FLUID. | |
| EP2413128A1 (en) | Improved microfluidic sensor units to detect the presence of an analyte in a sample-fluid | |
| CN106198128A (en) | A kind of air particle automatic acquisition device | |
| TWI498166B (en) | Porous membrane based autonormous handling process microfluidic device for surface plasmon resonance quantitative analysis | |
| IT201600104601A1 (en) | MICROFLUID SYSTEM | |
| CN104535780B (en) | The micro-fluidic chip of a kind of fixing microgranule, sensor and microgranule fixing means thereof | |
| CN104165906A (en) | Virus detector | |
| CN215783449U (en) | Self-driven micro-fluid detection card capable of controlling flow rate | |
| CN209446605U (en) | A kind of liquid stream control and measuring device based on hydrogel | |
| JP5892589B2 (en) | Microdevice and bioassay system | |
| CN106198499A (en) | A kind of micro-fluidic chip for chemiluminescence detection and detection method thereof | |
| CN105738637A (en) | Full-automatic capillary electrophoresis device suitable for separation analysis of space biological sample | |
| CN109752353A (en) | Microlayer model detection device | |
| CN203949924U (en) | For the biochip of quick magnetic immunoassay | |
| CN107216998B (en) | Automatic device for rapidly measuring bacterial content in oil field sewage and finished oil | |
| CN201837609U (en) | ORP detection bottle |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |