CN206609824U - A kind of cellulose acetate membrane electrophoresis sample applicator - Google Patents
A kind of cellulose acetate membrane electrophoresis sample applicator Download PDFInfo
- Publication number
- CN206609824U CN206609824U CN201720287184.3U CN201720287184U CN206609824U CN 206609824 U CN206609824 U CN 206609824U CN 201720287184 U CN201720287184 U CN 201720287184U CN 206609824 U CN206609824 U CN 206609824U
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- CN
- China
- Prior art keywords
- cross bar
- point template
- cellulose acetate
- shell
- point
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Abstract
The utility model provides a kind of cellulose acetate membrane electrophoresis sample applicator, including point template (5), cross bar (2), multiple elastic components (3), foam-rubber cushion (6), shell (7) and obstruction device (4), the cross bar (2) be arranged in parallel with point template (5), the multiple elastic component (3) uniform vertical is set in point template (5), and one end of the elastic component is connected with cross bar (2), the other end is connected with point template (5)；The top of the cross bar (2), elastic component (3) and point template (5) institute Special composition and it is surrounded by shell (7), the inwall of the shell (7) is used to hinder to be provided with foam-rubber cushion (6) below the obstruction device (4) that cross bar (2) is moved down, the outer wall of the shell (7) provided with least one.The utility model is simple in construction, easy to operate, and point sample dynamics is uniform, and absorption is obvious, and effect preferably, is easy to operation practical, can greatly improve the success rate of electrophoresis.
Description
Technical field
The utility model is related to a kind of drugs analysis equipment, particularly a kind of cellulose acetate membrane electrophoresis sample applicator.
Background technology
Electrophoresis is a kind of experimental technique commonly used in Biochemistry Experiment, is often used in entering the materials such as DNA and protein
Row Qualitative and quantitative analysis.Film is made as the electrophoresis apparatus referred to as film electrophoresis of support using acetate fiber etc..In certain acid
Under the conditions of basicity, protein molecule is because group is ionized and carries certain electric charge, on the film containing buffer solution, by electric field
Masterpiece is moved to certain speed to certain direction.Different protein molecules, by electrically charged difference, movement
Speed and direction are also different, so as to reach separation.Suction-operated of this film to protein example is small, and paper is almost completely eliminated
The conditions of streaking occurred in protein electrophorese, but because relatives' property of film is smaller, the buffer solution that it is accommodated is also few.Electrophoresis
The electric current for being is largely that sample is conducting, therefore with simply, quickly, zone is clear, and sensitivity is high, it is easy to quantifies and is easy to
The characteristics of preservation.This method amount of samples is few, and 5ug protein is with regard to that can obtain satisfied result.It is therefore particularly suited for pathology
In the case of trace of albumin detect.Analyzed with the separation for being widely used in protein and isodynamic enzyme, can be used for the side such as immunoelectrophoresis
Face.
Existing cellulose acetate membrane electrophoresis sample applicator is, in the point template both sides for picking sample, to be glued with same width
Spend but highly less except liquid plate, except liquid plate is connected by spring with support arm, it is ensured that remove liquid with tight promoted by anti-slip tank of finger
When plate picks solution downwards, both sides can be contacted with sample liquid simultaneously except liquid plate against middle point template, three plates, make the overwhelming majority molten
Liquid is pressed against beyond whole device, so as to avoid middle point template two sides from assembling more solution；In addition, finger unclamps after sampling
Due to the pulling force of spring, shrinking except liquid plate for both sides, simultaneously because support arm upper end is designed with handle, can be held and stop
Handle, the point sample on acetate film, so as to guarantee to point out one slightly, this sample applicator is simple in construction, and novel and unique is easy to
Operation, point sample effect is good, substantially increases operating efficiency, economical and practical, but there is also the dynamics of shortcoming, i.e. point sample by artificially controlling
System, and this dynamics needs to grasp by experience, is difficult to control, but cellulose acetate film is very thin, if firmly excessive, film is fragile；
If firmly too small, the line pointed out is not obvious.And it is bad except liquid effect, therefore improve existing cellulose acetate membrane electrophoresis point
Sample device has implementation necessary very much.
Utility model content
For above-mentioned situation, to overcome the defect of prior art, it is fine that the purpose of this utility model is just to provide a kind of acetic acid
Film electrophoresis sample applicator is tieed up, existing sample applicator inconvenient operation can be effectively solved, dynamics control is uneven, and adsorption effect is inadequate
It is good, the problem of experiment success rate is low.
To achieve these goals, the utility model uses following technical scheme：
A kind of cellulose acetate membrane electrophoresis sample applicator, including point template, in addition to it is cross bar, multiple elastic components, foam-rubber cushion, outer
Shell and obstruction device；The cross bar is be arranged in parallel with point template, and the multiple elastic component uniform vertical is set in point template, and described
One end of elastic component is connected with cross bar, and the other end is connected with point template；The cross bar, elastic component and point template institute Special composition
Top and be surrounded by shell, the inwall of the shell is used to hinder the obstruction device that cross bar is moved down, institute provided with least one
State and foam-rubber cushion is provided with below the outer wall of shell.
The cross bar is provided with handle, is easy to operation.
The obstruction device is preferably disposed on the outer casing inner wall of point template 1-2cm height.
The elastic component is preferably spring.
The cross bar and point template equal length.
The obstruction device is preferably two, and is symmetrical arranged and outer casing inner wall.
The obstruction device can hinder cross bar to move down, when spring is in elastic deformation, using the stretching of spring to point sample
Plate applies pulling force or pressure, so that control point model point sample dynamics.It is many that the foam-rubber cushion is dipped for adsorbing around point template
Remaining sample liquid.
The utility model structure is practical simple, easy to operate, novel and unique, and point sample dynamics is uniform, and absorption is obvious, effect compared with
It is good, it is easy to operation practical, the success rate of electrophoresis can be greatly improved, it is economical and practical.
Brief description of the drawings
Fig. 1 is positive structure schematic of the present utility model.
Fig. 2 is side structure schematic diagram of the present utility model.
Fig. 3 is overlooking the structure diagram of the present utility model.
Embodiment
The utility model is further described with reference to Figure of description and embodiment：
A kind of cellulose acetate membrane electrophoresis sample applicator, including point template 5, in addition to cross bar 2, multiple elastic components 3, foam-rubber cushion
6th, shell 7 and obstruction device 4；The cross bar 2 be arranged in parallel with point template 5, and the multiple uniform vertical of elastic component 3 is in point template 5
Set, and one end of the elastic component is connected with cross bar 2, the other end is connected with point template 5；The cross bar 2, elastic component 3 and
The top of 5 Special compositions of point template is used to hinder with shell 7, the inwall of the shell 7 is surrounded by provided with least one
Foam-rubber cushion 6 is provided with below the obstruction device 4 that cross bar 2 is moved down, the outer wall of the shell 7.
The cross bar 2 is provided with handle 1.The elastic component 3 is spring.It is described obstruction device 4 be two, and be symmetrical arranged with
Outer casing inner wall.
The cross bar 2 and the equal length of sample applicator 5, are equal to point sample width, spring is uniformly fixed on point template 5 and cross bar
On 2, its equally distributed effect is to make spring uniform force application.
The width for hindering device 4 is enough to hinder the combination of cross bar 2 and handle 1 to move down, when combination is in this position, point
The just uniform force point sample of model 5.
The uniform seal of described sample liquid is on acetate film, and dynamics is both not enough to crush acetate film, again
Be avoided that the point sample of point template 5 is too light, sample liquid very little the problem of.
The foam-rubber cushion 6 is arranged at below the outer wall of shell (bottom position below shell), when point template is pushed, sea
The continuous surrounding of 6 tight adhesion point template of pad 5, its role is to can adsorb the excessive sample dipped around point template 5 to greatest extent
Product liquid.
The spring-mass is lighter, and its weight is not enough to impact the point sample of point template 5, and is in whole process
In the range of elastic deformation, its power is directly proportional to deformation length during this.
The spring effect is, when pull-up handle 1 is combined with cross bar 2, spring elongation, overcome spring in itself with point template
5 microgravity, when pushing handle 1 and being combined with cross bar 2, spring-compressed plays point sample effect, and obstruction device 4 is combined to when pushing
Hinder to stop during position.
The position of described obstruction device 4 is fixed, and is L apart from cellulose acetate film distance, and the mass of point template 5 is m, bullet
Spring coefficient is k, the former a length of l of spring, if upward direction is positive direction.For the known point sample dynamics F of each different, there is one
Individual linear L is corresponded to therewith, and its corresponding relation is：L=l ± (mg-F)/4k this formula when spring elongation takes "-", when spring pressure
This formula takes "+" during contracting.
In embodiment, the mass of point template 5 is 6-10g, and spring constant is 1N/cm, the former a length of 3cm of spring, for point sample power
10-15N is spent, it hinders device to be 1-2cm apart from the distance of cellulose acetate film.
Concrete operation method：
Operating method of the present utility model is that experiment pushes handle of the present utility model before starting, and moves down point template
Sample liquid is dipped in sample cell, then upper handle, point template is freely increased, foam-rubber cushion absorbs sample unnecessary around point template
Product liquid.During experiment, handle of the present utility model is pushed, it is thin that the point template for having dipped sample liquid is vertically pressed in into cellulose acetate
On film, stop pushing after device resistance hindered is pushed, finally lift the utility model, can be in cellulose acetate film
Upper uniform point sample.
The utility model has plurality of specifications, can be needed during experiment according to experiment and the width of the fine film of vinegar selects suitable point sample
Device.
Described above, simply explained through diagrams some principles of the present utility model, and this specification is not intended to this practicality
It is new to be confined in shown described concrete structure and the scope of application, therefore every corresponding modification for being possible to be utilized and wait
Jljl, belongs to the apllied the scope of the claims of the utility model.
Claims (6)
1. a kind of cellulose acetate membrane electrophoresis sample applicator, including point template (5), it is characterized in that, in addition to cross bar (2), multiple bullets
Property part (3), foam-rubber cushion (6), shell (7) and hinder device (4)；The cross bar (2) be arranged in parallel with point template (5), the multiple
Elastic component (3) uniform vertical is set in point template (5), and one end of the elastic component is connected with cross bar (2), the other end and point sample
Plate (5) is connected；The top of the cross bar (2), elastic component (3) and point template (5) institute Special composition and it is surrounded by shell
(7), the inwall of the shell (7) is used to hinder the obstruction device (4) that cross bar (2) is moved down, the shell (7) provided with least one
Outer wall below be provided with foam-rubber cushion (6).
2. cellulose acetate membrane electrophoresis sample applicator according to claim 1, it is characterized in that, the cross bar (2) is provided with handle
(1)。
3. cellulose acetate membrane electrophoresis sample applicator according to claim 1, it is characterized in that, the obstruction device (4) be arranged at away from
On outer casing inner wall from point template (5) 1-2cm height.
4. cellulose acetate membrane electrophoresis sample applicator according to claim 1, it is characterized in that, the elastic component (3) is spring.
5. cellulose acetate membrane electrophoresis sample applicator according to claim 1, it is characterized in that, the cross bar (2) and point template (5)
Equal length.
6. according to one of the claim 1-5 cellulose acetate membrane electrophoresis sample applicators, it is characterized in that, the obstruction device (4) is
Two, and be symmetrical arranged and outer casing inner wall.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720287184.3U CN206609824U (en) | 2017-03-23 | 2017-03-23 | A kind of cellulose acetate membrane electrophoresis sample applicator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720287184.3U CN206609824U (en) | 2017-03-23 | 2017-03-23 | A kind of cellulose acetate membrane electrophoresis sample applicator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206609824U true CN206609824U (en) | 2017-11-03 |
Family
ID=60165592
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720287184.3U Expired - Fee Related CN206609824U (en) | 2017-03-23 | 2017-03-23 | A kind of cellulose acetate membrane electrophoresis sample applicator |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206609824U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110940720A (en) * | 2019-11-25 | 2020-03-31 | 徐州医科大学 | Automatic cellulose acetate film point appearance ware of pressing |
-
2017
- 2017-03-23 CN CN201720287184.3U patent/CN206609824U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110940720A (en) * | 2019-11-25 | 2020-03-31 | 徐州医科大学 | Automatic cellulose acetate film point appearance ware of pressing |
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|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171103 Termination date: 20200323 |
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| CF01 | Termination of patent right due to non-payment of annual fee |