CN108207970A

CN108207970A - A kind of bactericidal composition containing benziothiazolinone and pyrrole metsulfovax, pesticide and its application

Info

Publication number: CN108207970A
Application number: CN201810198232.0A
Authority: CN
Inventors: 王鹏; 吴泽和; 段新新; 翟卫兵
Original assignee: HUATE WESTERN SHAANXI SCIENCE AND TECHNOLOGY INDUSTRIAL Co Ltd
Current assignee: HUATE WESTERN SHAANXI SCIENCE AND TECHNOLOGY INDUSTRIAL Co Ltd
Priority date: 2015-08-31
Filing date: 2015-08-31
Publication date: 2018-06-29
Also published as: CN105211073A

Abstract

The invention discloses a kind of bactericidal composition containing benziothiazolinone and pyrrole metsulfovax, pesticide and its applications.The composition includes two kinds of active constituents of A, B, and component A is benziothiazolinone, and B component is pyrrole metsulfovax.A, the weight ratio between two components of B is 1：50-50：1, preferred weight ratio 1：10-10：1.Wettable powder, water dispersible granules, suspending agent, aqueous emulsion can be made in the composition, for preventing the downy mildew and epidemic disease of wheat rust, vegetables, melon and fruit class.

Description

A kind of bactericidal composition containing benziothiazolinone and pyrrole metsulfovax, pesticide and its application

The present invention is to be in August, 2015 patent application day 31, number of patent application 201510548282.3, entitled one Bactericidal composition of the kind containing benziothiazolinone and succinic acid Off hydrogen enzyme inhibitors, pesticide and its division Shen of the application for a patent for invention of application Please.

Technical field

The present invention relates to a kind of bactericidal compositions containing benziothiazolinone and pyrrole metsulfovax, belong to technical field of pesticide combination.

Technical background

Benziothiazolinone (Benziothiazolinone) belongs to isothiazole heterocyclic compound, is that a kind of efficient, less toxic, broad spectrum activity kills Microbial inoculum, mechanism of action are：It is reacted with bacterium, the anion binding of the cell membrane surface of mould or with sulfydryl, destroys protein With the synthesis system of cell membrane, so as to inhibit bacteria, mould breeding, interfere pathogen cell metabolism, make its physiologic derangement, Cause pathogen dead.The benziothiazolinone of Shaanxi Xida Huate Science ＆ Technology Industrial Co., Ltd.'s development and production obtains agriculture radical for 2008 Family's pesticide formal registration, and wide popularization and application.The compound has bacterium, fungus-caused corps diseases good prevention effect Fruit, and it is fool proof to crops.

Succinic acid Off hydrogen enzyme inhibitor series bactericidal agents (SDHI) are that one kind acts on pathogen succinic acid Off hydrogen enzymes, so as to press down The fungicide of its respiration is made, the effect of this series bactericidal agent is special, and strong drug action, efficiency time is long, and has effect of increasing production.At present Successfully developing this series bactericidal agent has 18 kinds, the new varieties of last decade listing mainly have thiophene methuroxam, Boscalid, pyrrole metsulfovax, Isopyrazam, biphenyl pyrrole metsulfovax, fluxapyroxad, fluorine azoles ring bacterium amine, fluorine azoles bacterium aniline, fluopyram, benzo alkene fluorine Bacterium azoles etc..The series bactericidal agent is found after being widely used, and since its action site is single, it is also beneficial convex that the medicament its resistance is used alone It is aobvious.

Invention content

The object of the present invention is to provide a kind of bactericidal composition, bactericidal effect gets well and can effectively improve succinic acid Off hydrogen enzymes The resistance problem of inhibitor series bactericidal agent, for this purpose, the present invention uses following technical scheme：

A kind of bactericidal composition containing benziothiazolinone and pyrrole metsulfovax, which is characterized in that the composition two kinds of activearms containing A, B Part, component A is benziothiazolinone, and B component is pyrrole metsulfovax, and the weight ratio of two kinds of active constituents of A, B is 1：50-50：1.

Further, the preferred weight ratio of two kinds of active constituents of A, B is 1：10-10：1.

Further, the sum of weight percent of two kinds of active constituents of A, B is 15%- 50% in bactericidal composition, It is remaining to complement to 100% for insecticides adjuvant.

Further, composition of the active material including the component A and B component can add corresponding pesticide and help Agent becomes wet powder, water dispersible granules, suspending agent, aqueous emulsion, as a kind of sterilization pesticide.

Further, the present invention provides the bactericidal composition in the frost for preparing prevention wheat rust, vegetables, melon and fruit class Application on the pesticide of mildew and epidemic disease.

Further, the present invention provides above-mentioned sterilization pesticide as the downy mildew for preventing wheat rust, vegetables, melon and fruit class With the application of the pesticide of epidemic disease.

Due to technical solution using the present invention, the benziothiazolinone and amber of bactericidal composition and one-component provided by the present invention Amber acid Off hydrogen enzyme inhibitor series bactericidal agents are compared, and have apparent sterilization synergistic effect, and can effectively improve succinic acid Off hydrogen enzymes The resistance problem of inhibitor series bactericidal agent.Wettable powder, water dispersible granules, suspending agent, aqueous emulsion can be made in the composition, For preventing the downy mildew and epidemic disease of wheat rust, vegetables, melon and fruit class.

Specific embodiment

With reference to embodiment, the present invention is further described, and the percentage of each component is weight percent in embodiment Than, but the present invention is not limited to the proportional region in following exemplary case.

First, dosage form preparating example

Embodiment 1,50% benziothiazolinone thifluzamide wettable powder

Benziothiazolinone 25%, thifluzamide 25%, fatty alcohol polyoxyethylene ether 10%, Nekal BX 6%, swelling scholar supply To 100%.By above-mentioned material in double worm mixer after mixing, 800 mesh are crushed to through airslide disintegrating mill hereinafter, being made Standard compliant 50% benziothiazolinone thifluzamide wettable powder.

Embodiment 2,45% benziothiazolinone Boscalid water dispersible granules.

Benziothiazolinone 20%, Boscalid 25%, alkyl phenol polyoxyethylene ether 7%, 6 % of lauryl sodium sulfate, ammonium sulfate 1%, aluminium oxide 0.2%, starch 5%, diatomite complements to 100%.Water slurry is made in above-mentioned material, which is used The granularity of particle adjusts suspending agent to 5um hereinafter, aqueous suspension agent is made in pipeline macerator pump and sand mill attrition grinding suspension The suspending agent is pumped into slush pump in special spray granulating and drying tower to proper ratio, is made and complies with standard by middle solid content 45% benziothiazolinone Boscalid water dispersible granules.

Embodiment 3,35% benziothiazolinone pyrrole metsulfovax suspending agent

Benziothiazolinone 17%, pyrrole metsulfovax 18%, fatty alcohol polyoxyethylene ether 7%, alkyl benzene calcium sulfonate 8%, polyethylene glycol 2%, silicone oil 0.2%, propylene glycol 3%, soft water complements to 100%.Suspension is made in above-mentioned material, by the suspension pipeline The granularity of particle is to 5um hereinafter, being made standard compliant, 35% thiophene is mould in macerator pump and sand mill attrition grinding suspension Ketone pyrrole metsulfovax suspending agent.

Embodiment 4,25% benziothiazolinone isopyrazam aqueous emulsion

Benziothiazolinone 12%, isopyrazam 13%, castor oil polyoxyethylene ether 3%, dodecyl pyrrolidone 1.5%, Glycerine 2.5%, silicone oil 0.2%, sodium benzoate 2%, soft water complements to 100%.Above-mentioned material is cut through high-shear emulsion machine Cutting obtains standard compliant 25% benziothiazolinone isopyrazam aqueous emulsion.

Embodiment 5,45% benziothiazolinone biphenyl pyrrole metsulfovax wettable powder

Benziothiazolinone 20%, biphenyl pyrrole metsulfovax 25%, fatty alcohol polyoxyethylene ether 10%, 6 % of Nekal BX, lightweight carbon Sour calcium complements to 100%.By above-mentioned material in double worm mixer after mixing, 800 mesh are crushed to through airslide disintegrating mill Hereinafter, standard compliant 45% benziothiazolinone biphenyl pyrrole metsulfovax wettable powder is made.

Embodiment 6,40% benziothiazolinone fluxapyroxad water dispersible granules

Benziothiazolinone 20%, fluxapyroxad 20%, alkyl phenol polyoxyethylene ether 8%, 5 % of lauryl sodium sulfate, sulfuric acid Ammonium 1%, aluminium oxide 0.2%, starch 5%, diatomite complements to 100%.Water slurry is made in above-mentioned material, by the suspension With the granularity of particle in pipeline macerator pump and sand mill attrition grinding suspension to 5um hereinafter, aqueous suspension agent is made, adjusting suspends The suspending agent is pumped into slush pump in special spray granulating and drying tower to proper ratio, is made and meets mark by solid content in agent 40% accurate benziothiazolinone fluxapyroxad water dispersible granules.

Embodiment 7,30% benziothiazolinone fluorine azoles ring bacterium amine suspending agent

Benziothiazolinone 15%, fluorine azoles ring bacterium amine 15%, fatty alcohol polyoxyethylene ether 7%, 8 % of alkyl benzene calcium sulfonate, poly- second two Alcohol 2%, silicone oil 0.2%, propylene glycol 3%, soft water complements to 100%.Suspension is made in above-mentioned material, which is managed The granularity of particle is to 5um hereinafter, obtained standard compliant 30% thiophene is mould in road macerator pump and sand mill attrition grinding suspension Ketone fluorine azoles ring bacterium amine suspending agent.

Embodiment 8,25% benziothiazolinone fluorine azoles bacterium aniline aqueous emulsion

Benziothiazolinone 12%, fluorine azoles bacterium aniline 13%, castor oil polyoxyethylene ether 3%, dodecyl pyrrolidone 1.5%, Glycerine 2.5%, silicone oil 0.2%, sodium benzoate 2%, soft water complements to 100%.Above-mentioned material is cut through high-shear emulsion machine Cutting obtains standard compliant 25% benziothiazolinone fluorine azoles bacterium aniline aqueous emulsion.

Embodiment 9,46% benziothiazolinone fluopyram wettable powder

Benziothiazolinone 23%, fluopyram 23%, fatty alcohol polyoxyethylene ether 9%, Nekal BX 7%, precipitated calcium carbonate Complement to 100%.By above-mentioned material in double worm mixer after mixing, be crushed to 800 mesh through airslide disintegrating mill hereinafter, Standard compliant 46% benziothiazolinone fluopyram wettable powder is made.

Embodiment 10,40% benziothiazolinone benzo alkene triflumizole water dispersant

Benziothiazolinone 20%, benzo alkene fluorine bacterium azoles 20%, alkyl phenol polyoxyethylene ether 8%, lauryl sodium sulfate 5%, sulphur Sour ammonium 1%, aluminium oxide 0.2%, starch 5%, diatomite complements to 100%.Water slurry is made in above-mentioned material, this is suspended For the granularity of particle to 5um hereinafter, aqueous suspension agent is made, adjusting is outstanding in liquid pipeline macerator pump and sand mill attrition grinding suspension The suspending agent is pumped into slush pump in special spray granulating and drying tower to proper ratio, is made and meets by solid content in floating agent 40% benziothiazolinone benzo alkene triflumizole water dispersant of standard.

2nd, raw test (one)

Benziothiazolinone respectively with thiophene methuroxam, Boscalid, pyrrole metsulfovax, isopyrazam, biphenyl pyrrole metsulfovax, fluorine azoles bacterium A kind of compounding in amide, fluorine azoles ring bacterium amine, fluorine azoles bacterium aniline, fluopyram or benzo alkene fluorine bacterium azoles is to cucumber downy mildew disease Bacterium toxicity test.

Subjects：Cucumber downy mildew germ

Test method：According to the incidence of the experiment grade scale investigation whole strain blade of cucumber, calculate disease index and prevent Control effect.Control effect is converted into niqueMin (y), liquid volume height (ug/mL) is converted into logarithm (X), with least square Concentration EC during method calculates virulence equation and inhibits₅₀, according to the toxicity index grade co-toxicity coefficient (CTC) of the abundant method calculating medicaments of Sun Yun.

Survey toxicity index (ATI)=(standard agent EC₅₀/ reagent agent EC₅₀)×100。

The toxicity index of the percentage composition+B medicaments of A in toxicity index × mixture of theoretical toxicity index (TTI)=A medicaments The percentage composition of B in × mixture.

Co-toxicity coefficient (CTC)=[mixture actual measurement toxicity index (ATI)/mixture theoretical toxicity index (TTI)] × 100.

As CTC ＜ 80, composition shows as antagonism；As 80 ＜ CTC ＜ 120, composition, which shows as being added, to be made With；As CTC ＞ 120, composition shows as synergistic effect.

(1) benziothiazolinone is compounded with thiophene methuroxam to cucumber downy mildew Toxicity Determination

Table 1, benziothiazolinone, thiophene methuroxam bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		8.90	100
Thiophene methuroxam (B)		10.65	82.98
						A:B	1：50	8.11	109.85	99.66	110.22
A:B	1：30	7.28	122.53	99.45	123.2
						A:B	1：10	6.32	141.14	98.45	143.41
A:B	1：1	5.83	153.01	91.49	167.25
						A:B	10：1	5.69	156.78	84.52	185.6
A:B	30：1	5.69	156.77	83.53	125.94
						A:B	50：1	9.31	95.81	83.31	115.03

By 1 result of table can See, benziothiazolinone and thiophene methuroxam compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) Value is between 143.41 to 185.6, and compound synergic effect is more notable in this proportional region.

(2) benziothiazolinone is compounded with Boscalid to cucumber downy mildew Toxicity Determination

Table 2, benziothiazolinone, Boscalid bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		9.40	100
Boscalid (B)		11.45	81.98
						A:B	1：50	9.15	102.72	99.65	103.10
A:B	1：30	7.63	123.53	99.42	124.05
						A:B	1：10	6.52	144.54	98.35	146.91
A:B	1：1	5.59	168.31	90.49	185.25
						A:B	10：1	5.93	158.78	83.52	189.60
A:B	30：1	9.22	102.06	82.53	123.94
						A:B	50：1	10.15	92.81	82.31	112.73

By 2 result of table can See, benziothiazolinone and thiophene methuroxam compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) Value is between 146.91 to 189.60, and compound synergic effect is more notable in this proportional region.

(3) benziothiazolinone is compounded with pyrrole metsulfovax to cucumber downy mildew Toxicity Determination

Table 3, benziothiazolinone, pyrrole metsulfovax bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		10.05	100
Pyrrole metsulfovax (B)		11.45	81.98
						A:B	1：50	9.15	102.72	99.65	103.10
A:B	1：30	7.63	123.53	99.42	124.05
						A:B	1：10	6.52	144.54	98.35	146.91
A:B	1：1	5.59	168.31	90.49	185.25
						A:B	10：1	5.93	158.78	83.52	189.60
A:B	30：1	9.22	102.06	82.53	123.94
						A:B	50：1	10.15	92.81	82.31	112.73

By 3 result of table can See, benziothiazolinone and pyrrole metsulfovax compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) Value is between 146.91 to 189.60, and compound synergic effect is more notable in this proportional region.

(4) benziothiazolinone is compounded with isopyrazam to cucumber downy mildew Toxicity Determination

Table 4, benziothiazolinone, isopyrazam bactericidal composition are to cucumber downy mildew The Toxicity Determination result

By 4 result of table can See, benziothiazolinone and pyrazoles bacterium amine compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) Value is between 141.90 to 145.23, and compound synergic effect is more notable in this proportional region.

(5) benziothiazolinone is compounded with biphenyl pyrrole metsulfovax to cucumber downy mildew Toxicity Determination

Table 5, benziothiazolinone, biphenyl pyrrole metsulfovax bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		10.59	100
Biphenyl pyrrole metsulfovax (B)		12.78	82.86
						A:B	1：50	10.13	104.54	99.66	104.10
A:B	1：30	8.64	122.57	99.45	124.05
						A:B	1：10	6.68	158.53	98.37	161.39
A:B	1：1	7.27	145.67	91.49	159.37
						A:B	10：1	7.92	133.71	84.52	169.60
A:B	30：1	10.43	101.53	83.42	121.94
						A:B	50：1	12.27	86.31	83.20	112.03

By 5 result of table can See, benziothiazolinone and biphenyl pyrrole metsulfovax compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 161.39 to 169.60, and compound synergic effect is more notable in this proportional region.

(6) benziothiazolinone is compounded with fluxapyroxad to cucumber downy mildew Toxicity Determination

Table 6, benziothiazolinone, fluxapyroxad bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		12.16	100
Fluxapyroxad (B)		15.31	79.45
						A:B	1：50	11.63	104.72	99.55	105.10
A:B	1：30	10.12	120.13	99.32	120.05
						A:B	1：10	8.73	139.23	98.15	141.90
A:B	1：1	8.13	149.87	89.69	167.25
						A:B	10：1	8.48	143.34	81.52	176.60
A:B	30：1	12.17	99.93	80.53	124.94
						A:B	50：1	13.43	90.54	79.84	113.73

By 6 result of table can See, benziothiazolinone and fluxapyroxad compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 141.90 to 176.60, and compound synergic effect is more notable in this proportional region.

(7) benziothiazolinone is compounded with fluorine azoles ring bacterium amine to cucumber downy mildew Toxicity Determination

Table 7, benziothiazolinone, fluorine azoles ring bacterium amine bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		11.35	100
Fluorine azoles ring bacterium amine (B)		13.80	82.25
						A:B	1：50	10.55	107.57	99.65	107.10
A:B	1：30	9.46	119.95	99.42	120.05
						A:B	1：10	8.68	130.53	98.35	142.91
A:B	1：1	8.20	138.31	91.19	155.25
						A:B	10：1	9.41	120.58	83.82	148.60
A:B	30：1	11.07	102.46	82.53	123.64
						A:B	50：1	12.14	93.51	82.01	112.63

By 7 result of table can See, benziothiazolinone and fluorine azoles ring bacterium amine compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 142.91 to 148.60, and compound synergic effect is more notable in this proportional region.

(8) benziothiazolinone is compounded with fluorine azoles bacterium aniline to cucumber downy mildew Toxicity Determination

Table 8, benziothiazolinone, fluorine azoles bacterium aniline bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		11.84	100
Fluorine azoles bacterium aniline (B)		10.65	110.84
						A:B	1：50	10.15	116.28	99.65	107.10
A:B	1：30	9.63	124.46	99.42	120.05
						A:B	1：10	7.52	159.91	98.38	142.90
A:B	1：1	7.19	166.42	91.49	151.25
						A:B	10：1	8.23	143.28	83.52	146.53
A:B	30：1	8.72	136.04	82.33	123.94
						A:B	50：1	10.15	116.74	82.61	113.73

By 8 result of table can See, benziothiazolinone and fluorine azoles bacterium aniline compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 142.90 to 146.53, and compound synergic effect is more notable in this proportional region

(9) benziothiazolinone is compounded with fluopyram to cucumber downy mildew Toxicity Determination

Table 9, benziothiazolinone, fluopyram bactericidal composition are to cucumber downy mildew The Toxicity Determination result

By 9 result of table can See, benziothiazolinone and fluopyram compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 144.64 to 149.63, and compound synergic effect is more notable in this proportional region

(10) benziothiazolinone is compounded with benzo alkene fluorine bacterium azoles to cucumber downy mildew Toxicity Determination

Table 10, benziothiazolinone, benzo alkene fluorine bacterium azoles bactericidal composition are to cucumber downy mildew The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		12.05	100
Benzo alkene fluorine bacterium azoles (B)		11.25	81.98
						A:B	1：50	10.15	101.73	99.64	104.99
A:B	1：30	10.63	122.52	99.41	125.35
						A:B	1：10	8.52	143.53	98.34	153.58
A:B	1：1	7.59	167.30	90.48	156.64
						A:B	10：1	8.93	157.77	83.51	146.04
A:B	30：1	9.22	101.05	82.52	121.94
						A:B	50：1	10.15	91.80	82.30	107.73

By 10 result of table can See, benziothiazolinone and benzo alkene fluorine bacterium azoles compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 153.58 to 146.04, when compound proportion is 1：When 1, co-toxicity coefficient (CTC) value is 156.64, is compared herein The compound synergic effect of example range is more notable.

3rd, raw test (two)

Benziothiazolinone respectively with thiophene methuroxam, isopyrazam, biphenyl pyrrole metsulfovax, fluxapyroxad or benzo alkene fluorine bacterium A kind of compounding in azoles measures potato late blight virulence.

For test method with raw test one, result of the test is shown in Table 11, table 12, table 13, table 14, table 15 respectively.

(1) benziothiazolinone is compounded with thiophene methuroxam to potato Dinner epidemic disease Toxicity Determinations

Table 11, benziothiazolinone, thiophene methuroxam bactericidal composition are to potato Dinner epidemic disease The Toxicity Determination results

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		6.88	100
Thiophene methuroxam (B)		5.27	130.48
						A:B	1：50	5.71	120.44	100.59	119.75
A:B	1：30	5.43	126.64	100.98	125.41
						A:B	1：10	4.62	148.80	102.77	144.79
A:B	1：1	3.31	207.52	115.24	180.07
						A:B	10：1	2.93	235.14	127.71	184.11
A:B	30：1	4.20	163.65	129.50	126.36
						A:B	50：1	4.67	147.21	129.88	113.34

By 11 result of table can See, benziothiazolinone and thiophene methuroxam compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) Value is between 144.79 to 184.11, and compound synergic effect is more notable in this proportional region

(2) benziothiazolinone is compounded with isopyrazam to potato Dinner epidemic disease Toxicity Determinations

Table 12, benziothiazolinone, isopyrazam bactericidal composition are to potato Dinner epidemic disease The Toxicity Determination results

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		5.57	100
Isopyrazam (B)		6.30	88.42
						A:B	1：50	4.77	116.73	99.76	117.15
A:B	1：30	4.61	120.77	9962	121.23
						A:B	1：10	4.20	132.53	9894	133.94
A:B	1：1	3.27	170.11	94.21	180.57
						A:B	10：1	3.17	175.46	89.48	196.01
A:B	30：1	4.90	113.64	88.80	127.97
						A:B	50：1	5.54	100.53	88.65	113.41

By 12 result of table can See, benziothiazolinone and isopyrazam compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 133.94 to 196.01, and compound synergic effect is more notable in this proportional region

(3) benziothiazolinone is compounded with biphenyl pyrrole metsulfovax to potato Dinner epidemic disease Toxicity Determinations

Table 13, benziothiazolinone, biphenyl pyrrole metsulfovax bactericidal composition are to potato Dinner epidemic disease The Toxicity Determination results

By 13 result of table can See, benziothiazolinone and biphenyl pyrrole metsulfovax compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 146.91 to 189.60, and synergistic effect is more notable in the range of this compound proportion.

(4) benziothiazolinone is compounded with fluxapyroxad to potato Dinner epidemic disease Toxicity Determinations

Table 14, benziothiazolinone, azoles fluorine bacterium amide bactericidal composition are to potato Dinner epidemic disease The Toxicity Determination results

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		5.88	100
Fluxapyroxad (B)		6.20	94.84
						A:B	1：50	4.81	113.04	99.89	113.16
A:B	1：30	3.57	122.23	99.83	122.40
						A:B	1：10	4.04	14412	98.53	144.68
A:B	1：1	5.20	164.53	97.42	168.88
						A:B	10：1	5.41	145.42	95.31	152.57
A:B	30：1	9.22	113.04	95.01	118.98
						A:B	50：1	10.15	108.66	94.94	114.45

By 14 result of table can See, benziothiazolinone and azoles fluorine bacterium amide compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 144.68 to 152.57, when compound proportion is 1：When 1, co-toxicity coefficient (CTC) value is up to 168.88, herein The effect of proportional region compound synergic is more notable.

(5) benziothiazolinone is compounded with benzo alkene fluorine bacterium azoles to potato Dinner epidemic disease Toxicity Determinations

Table 15, benziothiazolinone, benzo alkene fluorine bacterium azoles bactericidal composition are to potato Dinner epidemic disease The Toxicity Determination results

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		6.11	100
Benzo alkene fluorine bacterium azoles (B)		6.57	94.84
						A:B	1：50	5.30	113.04	99.89	115.41
A:B	1：30	4.51	122.23	99.83	135.71
						A:B	1：10	4.14	14412	98.53	148.41
A:B	1：1	3.61	164.53	97.42	175.67
						A:B	10：1	4.23	145.42	95.31	154.49
A:B	30：1	5.15	113.04	95.01	127.27
						A:B	50：1	5.36	108.66	94.94	122.35

By 15 result of table can See, benziothiazolinone and benzo alkene fluorine bacterium azoles compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 148.41 to 154.49, when compound proportion is 1：When 1, co-toxicity coefficient (CTC) value is up to 175.67, herein In the range of compound synergic effect it is more notable.

4th, raw test (three)

Benziothiazolinone respectively with one in Boscalid, pyrrole metsulfovax, fluorine azoles ring bacterium amine, fluorine azoles bacterium aniline or fluopyram Kind compounding measures wheat rust virulence.

For test method with raw test one, result of the test is shown in Table 16, table 17, table 18, table 19, table 20 respectively.

(1) benziothiazolinone compounds with Boscalid and wheat rust virulence is measured

Table 16, benziothiazolinone, Boscalid compounding are to wheat rust virulence measurement result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		7.67	100
Boscalid (B)		8.14	94.22
						A:B	1：50	6.81	112.60	99.88	1152.73
A:B	1：30	6.40	119.80	9981	120.13
						A:B	1：10	5.67	135.20	99.47	135.92
A:B	1：1	5.20	147.41	97.11	151.78
						A:B	10：1	5.55	138.12	94.62	154.77
A:B	30：1	6.45	118.88	94.41	125.17
						A:B	50：1	6.86	111.78	94.34	118.49

By 16 result of table can See, benziothiazolinone and Boscalid compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) Value is between 135.92 to 154.77, and compound synergic effect is more notable in this proportional region.

(2) benziothiazolinone compounds with pyrrole metsulfovax and wheat rust virulence is measured

Table 17, benziothiazolinone, pyrrole metsulfovax bactericidal composition are to wheat rust The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		7.91	100
Pyrrole metsulfovax (B)		7.67	103.12
						A:B	1：50	7.10	111.38	100.06	111.32
A:B	1：30	6.44	122.78	100.10	122.66
						A:B	1：10	5.60	141.17	100.27	140.77
A:B	1：1	4.62	171.05	101.56	168.42
						A:B	10：1	5.26	150.27	102.84	146.13
A:B	30：1	6.20	127.53	103.01	123.74
						A:B	50：1	6.61	119.63	103.05	112.03

By 17 result of table can See, benziothiazolinone and pyrrole metsulfovax compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) Value is between 140.77 to 146.13, when compound proportion is 1：When 1, co-toxicity coefficient (CTC) value is up to 168.42, in this ratio Compound synergic effect is more notable.

(3) benziothiazolinone compounds with fluorine azoles ring bacterium amine and wheat rust virulence is measured

Table 18, benziothiazolinone, fluorine azoles ring bacterium amine bactericidal composition are to wheat rust The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		7.51	100
Fluorine azoles ring bacterium amine (B)		8.25	90.91
						A:B	1：50	6.51	115.17	99.82	115.38
A:B	1：30	6.20	120.92	99.71	121.28
						A:B	1：10	5.47	137.03	99.17	138.17
A:B	1：1	4.30	174.24	95.45	182.53
						A:B	10：1	5.17	144.97	91.74	158.02
A:B	30：1	6.71	111.75	91.21	122.52
						A:B	50：1	6.97	107.58	91.09	118.10

By 18 result of table can See, benziothiazolinone and fluorine azoles ring bacterium amine compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 138.17 to 158.02, when compound proportion is 1：When 1, co-toxicity coefficient (CTC) value is up to 182.53, herein The effect of ratio compound synergic is more notable.

(4) benziothiazolinone compounds with fluorine azoles bacterium aniline and wheat rust virulence is measured

Table 19, benziothiazolinone, fluorine azoles bacterium aniline bactericidal composition are to wheat rust The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		7.92	100
Fluorine azoles bacterium aniline (B)		7.68	103.11
						A:B	1：50	6.97	113.60	100.06	113.54
A:B	1：30	6.31	125.46	100.10	125.34
						A:B	1：10	5.25	150.75	100.27	150.33
A:B	1：1	4.90	161.50	101.55	159.02
						A:B	10：1	5.31	149.05	102.83	144.94
A:B	30：1	6.65	119.06	103.01	115.58
						A:B	50：1	6.84	115.76	103.05	112.33

By 19 result of table can See, benziothiazolinone and fluorine azoles bacterium aniline compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 150.33 to 144.94, when compound proportion is 1：When 1, co-toxicity coefficient (CTC) value is up to 159.02, herein The effect of ratio compound synergic is more notable.

(5) benziothiazolinone compounds with fluopyram and wheat rust virulence is measured

Table 20, benziothiazolinone, fluopyram bactericidal composition are to wheat rust The Toxicity Determination result

Reagent agent	Proportioning	EC₅₀It is worth (mg/L)	ATI	TTI	CTC
						Benziothiazolinone (A)		7.50	100
Fluopyram (B)		7.95	94.34
						A:B	1：50	6.50	115.35	99.89	115.49
A:B	1：30	6.16	121.71	99.82	121.94
						A:B	1：10	5.11	146.67	99.48	147.44
A:B	1：1	4.55	164.68	97.17	169.48
						A:B	10：1	5.26	142.50	94.86	150.22
A:B	30：1	6.51	115.17	94.53	121.84
						A:B	50：1	720	104.15	94.46	110.27

By 20 result of table can See, benziothiazolinone and fluopyram compound proportion are 10：1 to 1：Between 10, co-toxicity coefficient (CTC) value is between 147.44 to 150.22, when compound proportion is 1：When 1, co-toxicity coefficient (CTC) value is up to 169.48, herein The effect of ratio compound synergic is more notable.

5th, field control effectiveness test verification (one)

Treatment agent：Benziothiazolinone and pyrrole metsulfovax, fluxapyroxad, fluopyram compound drug carry out prevention cucumber cream Mildew field efficacy check experiment, result are listed in table 21, table 22, table 23 respectively.

Test method

Field trial is carried out in Shaanxi Jingyang cucumber, and field management routinely carries out, each to test not using other pesticides Plot area is 50 square metres, and twice, water consumption is 30-50Kg/ mus for experiment dispenser, and interval 7d sprays are primary.

Investigation method records occurring degree with staging

Grade scale is as follows：

0 grade：It is disease-free

1 grade：Sorus area accounts for less than 5% whole leaf area；

3 grades：Sorus area accounts for the 6-10% of whole leaf area；

5 grades：Sorus area accounts for the 11-20% of whole leaf area；

7 grades：Sorus area accounts for the 21-90% of whole leaf area；

9 grades：Sorus area accounts for more than 90% whole leaf area；

Experiment is investigated 4 times altogether, and radix is investigated before medicine, 7d after the 1st medicine, and 7d and 14d carries out tune Check after the 2nd medicine.

Drug effect computational methods

21 benziothiazolinone of table and pyrrole metsulfovax compounding field control cucumber downy mildew drug effect check experiment result

It can be seen that the prevention of its prevention cucumber downy mildew after benziothiazolinone and pyrrole metsulfovax compounding from the result of the test of table 21 It is with obvious effects better than single dose, in the similary preventive effect complex preparation dosage only about half of single dose dosage, hence it is evident that reduce use Dose.

22 benziothiazolinone of table and fluxapyroxad compounding field control cucumber downy mildew drug effect check experiment result

From the result of the test of table 22 can be seen that benziothiazolinone and fluxapyroxad compounding after its prevent the anti-of cucumber downy mildew Control it is with obvious effects better than single dose, in the similary preventive effect complex preparation dosage only about half of single dose dosage or less than half, Significantly reduce dosage.

23 benziothiazolinone of table and fluopyram compounding field control cucumber downy mildew drug effect check experiment result

From the result of the test of table 23 can be seen that benziothiazolinone and fluopyram compounding after its prevent the anti-of cucumber downy mildew Control it is with obvious effects better than single dose, in the similary preventive effect complex preparation dosage only about half of single dose dosage, hence it is evident that reduce Dosage.

6th, field control effectiveness test (two)

Treatment agent：Benziothiazolinone and pyrrole metsulfovax, fluxapyroxad compound drug carry out prevention prevention capsicum epidemic disease field Drug effect check experiment, result are listed in table 24, table 25 respectively.

Test method：Experiment is located at Xianyang pepper planting area, and field management routinely carries out, not using other agricultures Medicine, each experimental plot area are 20 square metres, and twice, interval 7d sprays for experiment dispenser.

Investigation method

Each 5 points of cell random searching, every is investigated 3 plants, and occurring degree is recorded with staging.

Stage division (is classified) by symptom type

0 grade：Health is without disease；

1 grade：Overground part only leaf, fruit have scab；

3 grades：The aerial stem of plant, branch have brown rot spot；

5 grades：Basal part of stem has brown rot spot；

7 grades：The aerial stem of plant, branch have brown rot spot, and have part branch death；

9 grades：Complete stool is withered.

Drug effect computational methods

24 benziothiazolinone of table and pyrrole metsulfovax compounding field control prevention prevention capsicum epidemic disease drug effect check experiment result

It can be seen that prevention capsicum epidemic disease effect of its prevention after benziothiazolinone and pyrrole metsulfovax compounding from the result of the test of table 24 Fruit is substantially better than single dose, in the similary preventive effect complex preparation dosage only about half of single dose dosage, hence it is evident that reduce medication Amount.

25 benziothiazolinone of table and fluxapyroxad compounding field control capsicum epidemic disease drug effect check experiment result

It can be seen that the prevention capsicum epidemic disease of its prevention after benziothiazolinone and fluxapyroxad compounding from the result of the test of table 25 It is with obvious effects better than single dose, in the similary preventive effect complex preparation dosage only about half of single dose dosage, hence it is evident that reduce use Dose.

7th, field control effectiveness test (three)

Treatment agent：Benziothiazolinone and thifluzamide, Boscalid compounding prevention one field efficacy check experiment of wheat rust, Its result is listed in table 26, table 27 respectively.

Test method：Experiment is located at Xianyang, and field management routinely carries out, and not using other pesticides, respectively tests small Area's area is 20 square metres, and twice, interval 7d sprays for experiment dispenser.

Investigation method：Each 5 points of cell random searching, every is investigated 3 plants, and occurring degree is recorded with staging.

Stage division (is classified) by symptom type

0 grade：Disease-free spot；

1 grade：Leaf spot lesion is less than 1cm less than 5, length；

3 grades：Leaf spot lesion is more than 1cm less than 6-10, part scab length；

5 grades：Leaf spot lesion is linked to be piece less than 11-25, part scab, and lesion area accounts for leaf area 10- 25%；

7 grades：Leaf spot lesion 26 or more, scab are linked to be piece, and lesion area accounts for leaf area 26-50%；

9 grades：Scab is linked to be piece, and lesion area accounts for more than 50% leaf area or full leaf is withered；

Drug effect computational methods

26 benziothiazolinone of table and thiophene methuroxam compounding field control wheat rust drug effect check experiment result

It can be seen that the prevention effect of its prevention wheat rust after benziothiazolinone and thiophene methuroxam compounding from the result of the test of table 26 Fruit is substantially better than single dose, in the similary preventive effect complex preparation dosage only about half of single dose dosage or less than half, hence it is evident that Reduce dosage.

27 benziothiazolinone of table and Boscalid compounding field control wheat lure drug effect check experiment result

From the result of the test of table 27 can be seen that benziothiazolinone and Boscalid compounding after its prevent wheat the prevention of disease is lured to imitate Fruit is substantially better than single dose, in the similary preventive effect complex preparation dosage only about half of single dose dosage, hence it is evident that reduce medication Amount.

Structure feature the foregoing is merely specific embodiments of the present invention, but the present invention is not limited thereto, Ren Heben The technical staff in field in the field of the invention, all cover among protection scope of the present invention by the variation or modification made.

Claims

A kind of 1. bactericidal composition containing benziothiazolinone and pyrrole metsulfovax, which is characterized in that the composition two kinds of activearms containing A, B Part, component A is benziothiazolinone, and B component is pyrrole metsulfovax, and the weight ratio of two kinds of active constituents of A, B is 1：50-50：1.
2. bactericidal composition according to claim 1, which is characterized in that the preferred weight ratio of two kinds of active constituents of A, B is 1：10-10：1.
3. bactericidal composition according to claim 1, which is characterized in that two kinds of active constituents of A, B in bactericidal composition The sum of weight percent is 15%-50%, remaining complements to 100% for insecticides adjuvant.
4. bactericidal composition described in claim 1 is in the agriculture for preparing prevention wheat rust, vegetables, the downy mildew of melon and fruit class and epidemic disease Application on medicine.
5. a kind of sterilization pesticide, which is characterized in that it is wettable powder, water dispersible granules, suspending agent, aqueous emulsion, activity Substance includes component A described in claim 1 and B component.
6. pesticide of the sterilization pesticide as prevention wheat rust, vegetables, the downy mildew of melon and fruit class and epidemic disease described in claim 5 Application.