CN108207944A - A kind of wettable powder of trichoderma asperellum HN compositions and application - Google Patents
A kind of wettable powder of trichoderma asperellum HN compositions and application Download PDFInfo
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- CN108207944A CN108207944A CN201810056174.8A CN201810056174A CN108207944A CN 108207944 A CN108207944 A CN 108207944A CN 201810056174 A CN201810056174 A CN 201810056174A CN 108207944 A CN108207944 A CN 108207944A
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- Prior art keywords
- trichoderma asperellum
- compositions
- wettable powder
- mass ratio
- shallow tray
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Abstract
Wettable powder and application the present invention relates to a kind of trichoderma asperellum HN compositions, the wettable powder of trichoderma asperellum HN compositions therein are prepared by the following method：One：Spore liquid is made in trichoderma asperellum HN bacterial strains, is cultivated；Two：Spore suspension is made, the spore suspension is as shallow tray fermentation seed liquor；Three：Sterilization fermentation matrix and shallow tray fermentation seed liquor are mixed, culture is air-dried, crushed, and obtains trichoderma asperellum HN conidia powders；Four：By trichoderma asperellum HN conidia powders, biochemical fulvic acid, lignin, Dipel in mass ratio 55 70:10:3:20 27 ratio mixing, is configured to trichoderma asperellum HN compositions；Five：Wettable powder is made in trichoderma asperellum HN compositions.The present invention can promote the absorption and growth of Crop fertilizer, improve crop quality, improve soil, induction plant generates resistance, inhibits the growth and breeding of germ, nontoxic to people and animals, free from environmental pollution.
Description
First, technical field
The present invention relates to the bacteria agents of crop disease control, and in particular to a kind of trichoderma asperellum HN compositions it is wettable
Property pulvis and application.
2nd, background technology
At present in agricultural production, prevention diseases and pests of agronomic crop relies primarily on Chemical control methods, in control disease pest damage losses
While, it also brings disease pest drug resistance and rises the problems such as breaking out probability increase with disease pest.By promoting and applying biological control, object
The green prevention and controls technologies such as prevention, Scientific Usage of Drugs are managed, not only facilitate protection bio-diversity, pest and disease damage is reduced and breaks out probability, it is real
The sustainable control of existing pest and disease damage, and also help and mitigate disease pest damage losses, ensure grain good harvest and main farm produce matter
Measure the effective supply of safety.
Trichoderma asperellum belongs to Fungi Imperfecti door, hyphomycetales, trichoderma.It is that a kind of distribution is wide, breeding is fast, has higher biological and ecological methods to prevent plant disease, pests, and erosion valency
Value and to the insensitive biological and ecological methods to prevent plant disease, pests, and erosion of some broad-spectrum germicides beneficial to fungi, the characteristics of adaptable strong, has a broad antifungal spectrum, moreover it is possible to promote
Into plant growth, there is hyperparasitism, Competition, generate lyases, generate antagonist compound, inhibit or kill cause of disease
The effects that bacterium, induction host plant are to the defense response of germ mode, has important economic implications.
3rd, invention content
It is an object of the present invention to provide a kind of wettable powder of trichoderma asperellum HN compositions, this trichoderma asperellum HN groups
The wettable powder for closing object solve that the chemical pesticide that uses at present generates when prevention prevents diseases and pests of agronomic crop to people and animals
It is toxic, environment is polluted, has the problem of residual, it is a further object to provide the wettable of this trichoderma asperellum HN compositions
The application of property pulvis.
To achieve these goals, the specific technical solution that the present invention uses is as follows：This trichoderma asperellum HN compositions
Wettable powder is prepared by the following method：
Step 1：By trichoderma asperellum HN inoculations in PDA slant mediums, 28 DEG C of culture 72-96 h are washed with aseptic distillation
Lower conidium, is made spore liquid, and spore liquid miospore number is 106A/mL, then by the 1% of its volume by PD culture solutions
It accesses in the PD culture solutions of sterilizing, is put into 28 DEG C, 120rpm culture 36-48h obtain first order seed culture solution；
Step 2：By wheat bran, diatomite, rice husk, water in mass ratio 2:1:1:4 ratio mixing, after stirring evenly, is packed into
200mL plastic bottles, charge weight are the 1/2 of plastics bottle height, and then 121 DEG C of 20 min of high pressure moist heat sterilization again, connect after cooling by 1%
Kind amount accesses first order seed culture solution in plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, and humidity 80% cultivates 5-7 d；It will
The fermenting microbe of plastic bottle is in mass ratio：1：500-1：1000 add in sterile water, are stirred into making spore suspension, should
Spore suspension is as shallow tray fermentation seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin, sterile water in mass ratio 50：25：1：50 stirrings
After uniformly, it is sub-packed in polybag, it is for use after 20~30 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation workshop
In, to solid fermentation workshop formaldehyde and closed 24 h of suffocating sterilization of potassium permanganate mixed liquor, then by sterilization fermentation matrix
With shallow tray fermentation seed liquor in mass ratio 10：1 ratio mixing, uniformly sprawls in seedling-cultivation plate, spreads to 1-2cm of thickness,
28 DEG C of constant temperature under conditions of moisturizing RH >=80%, cultivates 7d, then pours into desinfection chamber and carry out air-drying shallow tray fermentation product, air-dries
Shallow tray fermentation product afterwards is put into low-temperature grinding instrument after crushing, crosses 200 mesh sieve, and collection obtains trichoderma asperellum HN conidia powders；
Step 4：The trichoderma asperellum HN conidia powders of step 3 preparation, biochemical fulvic acid, lignin, Dipel are pressed into quality
Compare 55-70:10:3:The ratio of 20-27 mixes, and obtains being configured to trichoderma asperellum HN compositions after mixing.
Step 5：By above-mentioned trichoderma asperellum HN compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright
Powder in mass ratio 50：6：1：16：6：9 mixing are configured to the wettable powder of trichoderma asperellum HN compositions.
The application of the wettable powder of above-mentioned trichoderma asperellum HN compositions：By the wettable of the trichoderma asperellum HN compositions
Property pulvis spray or pouring root is in crops, for preventing crops soil-borne disease, root rot, stem rot, gray mold, killing farming
Any one in object lepidoptera pest.
Advantageous effect：
1st, the present invention can promote the absorption and plant growth of Crop fertilizer, improve crop product quality, improve soil, induction
Plant generates resistance, inhibits the growth and breeding of germ, nontoxic to people and animals, free from environmental pollution.
2nd, the present invention can effectively play the drug effect of biological pesticide, can prevent crops soil-borne disease, root rot, stem
Corruption, gray mold, powdery mildew, downy mildew and the cabbage caterpillar of crop, diamondback moth, sweet tea moth, prodenia litura, lopper worm, oriental tobacco budworm, jade
Rice snout moth's larva, rice leaf roller, striped rice borer.
4th, specific embodiment
The present invention is described further below：
Embodiment 1：
The wettable powder of this trichoderma asperellum HN compositions is prepared by following preparation method：
Step 1：By trichoderma asperellum HN inoculations in PDA slant mediums, 28 DEG C of culture 72h wash lower point with aseptic distillation
Raw spore, is made spore liquid, and spore liquid miospore number is 106A/mL, then by 1%（Volume ratio）The PD cultures of access sterilizing
In liquid, 28 DEG C are put into, 120rpm culture 36h obtain first order seed culture solution.
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring evenly, dress
Enter 200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, then 121 DEG C of 20 min of high pressure moist heat sterilization again, and 1% is pressed after cooling
Inoculum concentration accesses first order seed culture solution in plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, and humidity 80% cultivates 6d；It will
The fermenting microbe of plastic bottle is in mass ratio：1：500 add in sterile water, are stirred into making spore suspension, which hangs
Supernatant liquid is as shallow tray fermentation seed liquor；It is known as material, 1% inoculum concentration in the present invention after wheat bran, diatomite, rice husk, water mixing
It is the volume of the first order seed culture solution referred to the accession to and the mass ratio of material in plastic bottle.
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterile water（50：25：1：50）（Quality
Than）After stirring evenly, it is sub-packed in polybag, it is for use after 20 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation vehicle
Between in, to solid fermentation workshop formaldehyde and potassium permanganate（2:1）（Mass ratio）The closed suffocating sterilization of mixed liquor for 24 hours, then will
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate,
Paving under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, cultivates 7d, then pour into desinfection chamber and carry out air-drying tray to thickness 1cm
Tunning, the shallow tray fermentation product after air-drying are put into low-temperature grinding instrument after crushing, cross 200 mesh sieve, and collection obtains spine spore wood
Mould HN conidia powders.
Step 4：Above-mentioned trichoderma asperellum HN conidia powders, biochemical fulvic acid, lignin, Dipel are pressed（55：10：3：
20）Mass ratio mixes, and is configured to trichoderma asperellum HN compositions.
Step 5：By above-mentioned trichoderma asperellum HN compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright
Powder presses 50：6：1：16：6：9 mass ratioes mix, and are configured to the wettable powder of trichoderma asperellum HN compositions.
The wettable powder of the present embodiment trichoderma asperellum HN compositions is directly used in corps leaf surface and sprays prevention tomato
Powdery mildew, downy mildew, gray mold, Chinese cabbage cabbage caterpillar, diamondback moth, sweet tea moth, prodenia litura.
Embodiment 2：
The wettable powder of this trichoderma asperellum HN compositions is prepared by following preparation method：
Step 1：By trichoderma asperellum HN inoculations in PDA slant mediums, 28 DEG C of culture 72h wash lower point with aseptic distillation
Raw spore, is made spore liquid, and spore liquid miospore number is 106A/mL, then by 1%（Volume ratio）The PD cultures of access sterilizing
In liquid, 28 DEG C are put into, 120rpm cultures 36h.
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring evenly, dress
Enter 200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, then 121 DEG C of 20 min of high pressure moist heat sterilization again, and 1% is pressed after cooling
It in inoculum concentration access plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, humidity 80% cultivates 6d；By the fermenting microbe of plastic bottle
In mass ratio：1：500 add in sterile water, are stirred into making spore suspension, the spore suspension is as solid tray
Fermentation seed liquid.
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterile water（50：25：1：50）（Quality
Than）After stirring evenly, it is sub-packed in polybag, it is for use after 20 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation vehicle
Between in, to solid fermentation workshop formaldehyde and potassium permanganate（2:1）（Mass ratio）The closed suffocating sterilization of mixed liquor for 24 hours, then will
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate,
Paving under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, cultivates 7d, then pour into desinfection chamber and carry out air-drying tray to thickness 1cm
Tunning, the shallow tray fermentation product after air-drying are put into low-temperature grinding instrument after crushing, cross 200 mesh sieve, and collection obtains spine spore wood
Mould HN conidia powders.
Step 4：Above-mentioned trichoderma asperellum HN conidia powders, biochemical fulvic acid, lignin, Dipel are pressed（70：10：3：
27）Mass ratio mixes, and is configured to trichoderma asperellum HN compositions.
Step 5：By above-mentioned trichoderma asperellum HN compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright
Powder presses 50：6：1：16：6：9 mass ratioes mix, and are configured to the wettable powder of trichoderma asperellum HN compositions.
The wettable powder of the present embodiment trichoderma asperellum HN compositions is directly used in crops leaching filling can prevent tomato
Powdery mildew, downy mildew, gray mold, Chinese cabbage cabbage caterpillar, diamondback moth, sweet tea moth, prodenia litura.
Embodiment 3：
The wettable powder of this trichoderma asperellum HN compositions is prepared by following preparation method：
Step 1：By trichoderma asperellum HN inoculations in PDA slant mediums, 28 DEG C of culture 72h wash lower point with aseptic distillation
Raw spore, is made spore liquid, and spore liquid miospore number is 106A/mL, then by 1%（Volume ratio）The PD cultures of access sterilizing
In liquid, 28 DEG C are put into, 120rpm cultures 36h.
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring evenly, dress
Enter 200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, then 121 DEG C of 20 min of high pressure moist heat sterilization again, and 1% is pressed after cooling
It in inoculum concentration access plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, humidity 80% cultivates 6d；By the fermenting microbe of plastic bottle
In mass ratio：1：500 add in sterile water, are stirred into making spore suspension, the spore suspension is as solid tray
Fermentation seed liquid.
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterile water（50：25：1：50）（Quality
Than）After stirring evenly, it is sub-packed in polybag, it is for use after 20 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation vehicle
Between in, to solid fermentation workshop formaldehyde and potassium permanganate（2:1）（Mass ratio）The closed suffocating sterilization of mixed liquor for 24 hours, then will
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate,
Paving under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, cultivates 7d, then pour into desinfection chamber and carry out air-drying tray to thickness 2cm
Tunning, the shallow tray fermentation product after air-drying are put into low-temperature grinding instrument after crushing, cross 200 mesh sieve, and collection obtains spine spore wood
Mould HN conidia powders.
Step 4：Above-mentioned trichoderma asperellum HN conidia powders, biochemical fulvic acid, lignin, Dipel are pressed（60：10：3：
27）Mass ratio mixes, and is configured to trichoderma asperellum HN compositions.
Step 5：By above-mentioned trichoderma asperellum HN compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright
Powder presses 50：6：1：16：6：9 mass ratioes mix, and are configured to the wettable powder of trichoderma asperellum HN compositions.
The wettable powder of the present embodiment trichoderma asperellum HN compositions is directly used in pouring root can prevent root rot.
Claims (2)
1. a kind of wettable powder of trichoderma asperellum HN compositions, it is characterised in that：This trichoderma asperellum HN compositions it is wettable
Property pulvis is prepared by the following method：
Step 1：By trichoderma asperellum HN inoculations in PDA slant mediums, 28 DEG C of culture 72-96 h are washed with aseptic distillation
Lower conidium, is made spore liquid, and spore liquid miospore number is 106A/mL, then by the 1% of its volume by PD culture solutions
It accesses in the PD culture solutions of sterilizing, is put into 28 DEG C, 120rpm culture 36-48h obtain first order seed culture solution；
Step 2：By wheat bran, diatomite, rice husk, water in mass ratio 2:1:1:4 ratio mixing, after stirring evenly, is packed into
200mL plastic bottles, charge weight are the 1/2 of plastics bottle height, and then 121 DEG C of 20 min of high pressure moist heat sterilization again, connect after cooling by 1%
Kind amount accesses first order seed culture solution in plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, and humidity 80% cultivates 5-7 d；It will
The fermenting microbe of plastic bottle is in mass ratio：1：500-1：1000 add in sterile water, are stirred into making spore suspension, should
Spore suspension is as shallow tray fermentation seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin, sterile water in mass ratio 50：25：1：50 stirrings
After uniformly, it is sub-packed in polybag, it is for use after 20~30 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation workshop
In, to solid fermentation workshop formaldehyde and closed 24 h of suffocating sterilization of potassium permanganate mixed liquor, then by sterilization fermentation matrix
With shallow tray fermentation seed liquor in mass ratio 10：1 ratio mixing, uniformly sprawls in seedling-cultivation plate, spreads to 1-2cm of thickness,
28 DEG C of constant temperature under conditions of moisturizing RH >=80%, cultivates 7d, then pours into desinfection chamber and carry out air-drying shallow tray fermentation product, air-dries
Shallow tray fermentation product afterwards is put into low-temperature grinding instrument after crushing, crosses 200 mesh sieve, and collection obtains trichoderma asperellum HN conidia powders；
Step 4：The trichoderma asperellum HN conidia powders of step 3 preparation, biochemical fulvic acid, lignin, Dipel are pressed into quality
Compare 55-70:10:3:The ratio of 20-27 mixes, and obtains being configured to trichoderma asperellum HN compositions after mixing；
Step 5：By above-mentioned trichoderma asperellum HN compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, press without bright powder
Mass ratio 50：6：1：16：6：9 mixing are configured to the wettable powder of trichoderma asperellum HN compositions.
2. a kind of application of the wettable powder of trichoderma asperellum HN compositions described in claim 1, it is characterised in that：By described in
The wettable powders of trichoderma asperellum HN compositions spray or pouring root is in crops, for preventing crops soil-borne disease, root
Maize ear rot, gray mold, kills any one in crops lepidoptera pest at stem rot.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810056174.8A CN108207944A (en) | 2018-01-20 | 2018-01-20 | A kind of wettable powder of trichoderma asperellum HN compositions and application |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810056174.8A CN108207944A (en) | 2018-01-20 | 2018-01-20 | A kind of wettable powder of trichoderma asperellum HN compositions and application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109517744A (en) * | 2019-01-10 | 2019-03-26 | 海南大学 | The composite spore powder and its application of Trichoderma mixed cooperative solid fermentation preparation |
| CN114304190A (en) * | 2021-09-29 | 2022-04-12 | 海南大学 | Preparation method of trichoderma wettable powder |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107114411A (en) * | 2017-06-07 | 2017-09-01 | 刘铜 | For the trichoderma asperellum DQ3 compound powders YBY of raising rice seedlings and application |
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2018
- 2018-01-20 CN CN201810056174.8A patent/CN108207944A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107114411A (en) * | 2017-06-07 | 2017-09-01 | 刘铜 | For the trichoderma asperellum DQ3 compound powders YBY of raising rice seedlings and application |
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| 尹杰 等: "《哪里不懂查哪里 高中生物》", 31 August 2014, 湖南师范大学出版社：湖南大学出版社，第1版 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109517744A (en) * | 2019-01-10 | 2019-03-26 | 海南大学 | The composite spore powder and its application of Trichoderma mixed cooperative solid fermentation preparation |
| CN114304190A (en) * | 2021-09-29 | 2022-04-12 | 海南大学 | Preparation method of trichoderma wettable powder |
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