CN107006524A - A kind of long shoot trichoderma DQ2 wettable powders are prepared and applied - Google Patents
A kind of long shoot trichoderma DQ2 wettable powders are prepared and applied Download PDFInfo
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- CN107006524A CN107006524A CN201710424515.8A CN201710424515A CN107006524A CN 107006524 A CN107006524 A CN 107006524A CN 201710424515 A CN201710424515 A CN 201710424515A CN 107006524 A CN107006524 A CN 107006524A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Pest Control & Pesticides (AREA)
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- Agronomy & Crop Science (AREA)
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Abstract
Prepare and its apply the present invention relates to a kind of long shoot trichoderma DQ2 wettable powders, wherein long shoot trichoderma DQ2 wettable powders are prepared from by following preparation method：Step one：By long shoot trichoderma DQ2 inoculations in PDA slant medium spore liquids, then it is linked into the PD nutrient solutions of sterilizing and is cultivated；Step 2：Spore suspension is made, the spore suspension is used as shallow tray fermentation seed liquor；Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin, sterilized water in mass ratio 50：25：1：After 50 stir, it is sub-packed in polybag and sterilizes, then mix sterilization fermentation matrix and shallow tray fermentation seed liquor, culture, the shallow tray fermentation product after air-drying is crushed, and obtains long shoot trichoderma DQ2 conidia powders；Step 4：It is configured to long shoot trichoderma DQ2 wettable powders.The present invention can promote the absorption and plant growth of fertilizer, nontoxic to people and animals, free from environmental pollution.
Description
First, technical field
The present invention relates to the bacteria agent of crop disease control, and in particular to a kind of long shoot trichoderma DQ2 wettable powders
Prepare and its apply.
2nd, background technology
As the proportion of crop planting time limit increases, cropping pattern is relatively fixed, and causes to have accumulated substantial amounts of harmful organism, disease in soil
Occur increasingly to weigh, soil is cultivated year after year in addition, causes soil physical properties substantially to change, a large amount of hardened, fertilizer occurs in soil
Power and cultivated power decline, and finally result in crop yield, quality and significantly decline, thus soil-borne disease and soil fertility decline into
To influence the important limiting factor of production estimation.But preventing and treating corps diseases rely primarily on chemical means, many institute's weeks at present
Know, chemical pesticide has residual in crop, empty gas and water and soil, causes residues of pesticides exceeded and environmental pollution, and single medicine
The use of agent easily causes disease pest to develop immunity to drugs, while also murdering the beneficial microbe of some in soil, reduces soil beneficial
Micropopulation, reduces soil organic matter content, reduces fertility and causes soil hardening, and many societies, warp are brought to this
Ji and ecological problem.With the improvement of people ' s living standards with the enhancing of environmental consciousness, requirement of the public to green vegetables also with
Day all increasings.Meanwhile, also need green vegetables in call the need for the export of farm produce.
Long shoot trichoderma belongs to Fungi Imperfecti door, hyphomycetales, trichoderma.It is that class distribution is wide, breeding is fast, with higher biological and ecological methods to prevent plant disease, pests, and erosion valency
Value and to the beneficial fungi of the insensitive biological and ecological methods to prevent plant disease, pests, and erosion of some broad-spectrum germicides, the characteristics of with strong adaptability, has a broad antifungal spectrum, moreover it is possible to promote
Enter plant growth, with hyperparasitism, Competition, produce lyases, generation antagonist compound, suppress or kill cause of disease
Bacterium, induction host plant are to modes of action such as the defense responses of germ, with important economic implications.
3rd, the content of the invention
It is an object of the present invention to provide a kind of preparation of long shoot trichoderma DQ2 wettable powders, this long shoot trichoderma DQ2 is wettable
Property pulvis having to people and animals of solving that the chemical pesticide that uses at present produces when preventing and treating crop root part disease and soil-borne disease
Poison, pollution environment, the problem of having residual, it is a further object to provide this long shoot trichoderma DQ2 wettable powders
Using.
To achieve these goals, the concrete technical scheme that the present invention is used is as follows：This long shoot trichoderma DQ2 wettable powders
Agent is prepared from by following preparation method：
Step one：By long shoot trichoderma DQ2 inoculations in PDA slant mediums, 28 DEG C of culture 72-96 h use sterile purified water
Lower conidium is washed, spore liquid is made, spore liquid miospore number is 106Individual/mL, then by its volume for pressing PD nutrient solutions
1% is linked into the PD nutrient solutions of sterilizing, in 28 DEG C, 120rpm cultures 36-48h；
Step 2：By wheat bran, diatomite, rice husk, water in mass ratio 2:1:1:4 ratio mixing, after stirring, loads
200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, and then 121 DEG C of min of high pressure moist heat sterilization 20 again, connect after cooling by 1%
Plant in amount access plastic bottle, shake uniform up and down, be placed on 28 DEG C of temperature, the culture 5-7 of humidity 80% d；By the fermented bacterium of plastic bottle
In mass ratio：1：500-1：1000 add in sterilized water, are stirred into making spore suspension, and the spore suspension is as solid
Body shallow tray fermentation seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin, sterilized water in mass ratio 50：25：1：50 stirrings
After uniform, it is sub-packed in polybag, in stand-by after 20~30 min that sterilized at 121 DEG C；Seedling-cultivation plate is arranged at solid fermentation workshop
In, to solid fermentation workshop formaldehyde and the closed h of suffocating sterilization 24 of potassium permanganate mixed liquor, then by sterilization fermentation matrix
With shallow tray fermentation seed liquor in mass ratio 10：1 ratio mixing, is uniformly sprawled in seedling-cultivation plate, the paving to -2cm of thickness 1,
Under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, 7d is cultivated, then pours into the air-dried shallow tray fermentation product of progress in desinfection chamber, is air-dried
Shallow tray fermentation product afterwards is put into low-temperature grinding instrument after crushing, crosses 200 mesh sieves, and collection obtains long shoot trichoderma DQ2 conidia powders；
Step 4：Above-mentioned long shoot trichoderma DQ2 conidia powders, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright powder press matter
Amount compares 50：6：1：16：6：9 mixing, are configured to long shoot trichoderma DQ2 wettable powders.
The application of above-mentioned long shoot trichoderma DQ2 wettable powders：Described long shoot trichoderma DQ2 wettable powders are directly used in
Foliage-spray preventing and treating muskmelon, tomato powdery mildew, downy mildew, gray mold.
The application of above-mentioned long shoot trichoderma DQ2 wettable powders：Described long shoot trichoderma DQ2 wettable powders are used to fill
Root, can be used for preventing and treating soil-borne diseases of vegetable, soil-borne diseases of vegetable includes root rot, stem rot, gray mold.
Beneficial effect：
1st, the present invention can promote the absorption and plant growth of fertilizer, improve crop product quality, improved soil, induction plant production
Raw resistance, suppresses the growth and breeding of harmful bacteria, nontoxic to people and animals, free from environmental pollution.
2nd, the present invention, which has, quickly occupies ecological niche advantage, produces antibiosis secondary metabolites, effectively preventing and treating vegetables soil biography
Disease, such as root rot, stem rot, gray mold.
4th, embodiment
The present invention is described further below：
Embodiment 1：
This long shoot trichoderma DQ2 wettable powders are prepared from by following preparation method：
Step one：By long shoot trichoderma DQ2 inoculations in PDA slant mediums, 28 DEG C of culture 72h are washed down with aseptic distillation
Conidium, is made spore liquid, and spore liquid miospore number is 106Individual/mL, then by 1%（Volume ratio）The PD trainings of access sterilizing
In nutrient solution, 28 DEG C, 120rpm cultures 36h are put into；
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring, load
200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, and then 121 DEG C of min of high pressure moist heat sterilization 20 again, connect after cooling by 1%
Plant in amount access plastic bottle, shake uniform up and down, be placed on 28 DEG C of temperature, humidity 80% cultivates 6 d；The fermented bacterium of plastic bottle is pressed
Mass ratio：1：500 are added in sterilized water, are stirred into making spore suspension, and the spore suspension is sent out as solid tray
Ferment seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterilized water（50：25：1：50）（Mass ratio）
After stirring, it is sub-packed in polybag, in stand-by after 20 min that sterilized at 121 DEG C；Seedling-cultivation plate is arranged at solid fermentation workshop
In, to solid fermentation workshop formaldehyde and potassium permanganate mixed liquor（Formaldehyde and potassium permanganate in formaldehyde and potassium permanganate mixed liquor
Mass ratio be 2:1）The closed h of suffocating sterilization 24, then presses 10 by sterilization fermentation matrix and shallow tray fermentation seed liquor：1
（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate, paving is to the cm of thickness 1, in 28 DEG C of constant temperature, the bar of moisturizing RH >=80%
Under part, 7d is cultivated, then pours into the air-dried shallow tray fermentation product of progress in desinfection chamber, the shallow tray fermentation product after air-drying is put into low temperature powder
After being crushed in broken instrument, 200 mesh sieves are crossed, collection obtains long shoot trichoderma DQ2 conidia powders.
Step 4：Above-mentioned long shoot trichoderma DQ2 conidia powders, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright powder
（50：6：1：16：6：9）（Mass ratio）Mixing, is configured to long shoot trichoderma DQ2 wettable powders.
The present embodiment long shoot trichoderma DQ2 wettable powders are directly used in foliage-spray preventing and treating muskmelon, tomato powdery mildew, frost
Mildew, gray mold.
Embodiment 2：
This long shoot trichoderma DQ2 wettable powders are prepared from by following preparation method：
Step one：By long shoot trichoderma DQ2 inoculations in PDA slant mediums, 28 DEG C of 96 h of culture are washed down with aseptic distillation
Conidium, is made spore liquid, and spore liquid miospore number is 106Individual/mL, then by 1%（Volume ratio）The PD trainings of access sterilizing
In nutrient solution, 28 DEG C, 120rpm cultures 40h are put into；
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring, load
200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, and then 121 DEG C of min of high pressure moist heat sterilization 20 again, connect after cooling by 1%
Plant in amount access plastic bottle, shake uniform up and down, be placed on 28 DEG C of temperature, humidity 80% cultivates 7 d；The fermented bacterium of plastic bottle is pressed
Mass ratio：1：800 are added in sterilized water, are stirred into making spore suspension, and the spore suspension is sent out as solid tray
Ferment seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterilized water（50：25：1：50）（Mass ratio）
After stirring, it is sub-packed in polybag, in stand-by after 30 min that sterilized at 121 DEG C；Seedling-cultivation plate is arranged at solid fermentation workshop
In, to solid fermentation workshop formaldehyde and potassium permanganate mixed liquor（2:1）（Mass ratio）The closed h of suffocating sterilization 24, then by
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate, spread
To thickness 2cm, under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, 7d is cultivated, then pours into desinfection chamber and carries out air-drying tray hair
Ferment product, the shallow tray fermentation product after air-drying is put into low-temperature grinding instrument after crushing, crosses 200 mesh sieves, and collection obtains long shoot trichoderma
DQ2 conidia powders.
Step 4：Above-mentioned long shoot trichoderma DQ2 conidia powders, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright powder
（50：6：1：16：6：9）（Mass ratio）Mixing, is configured to long shoot trichoderma DQ2 wettable powders.
Long shoot trichoderma DQ2 wettable powders manufactured in the present embodiment are used for pouring root, for preventing and treating soil-borne diseases of vegetable, root
Maize ear rot, stem rot, gray mold.
Embodiment 3：
This long shoot trichoderma DQ2 wettable powders are prepared from by following preparation method：
Step one：By long shoot trichoderma DQ2 inoculations in PDA slant mediums, 28 DEG C of 84 h of culture are washed down with aseptic distillation
Conidium, is made spore liquid, and spore liquid miospore number is 106Individual/mL, then by 1%（Volume ratio）The PD trainings of access sterilizing
In nutrient solution, 28 DEG C, 120rpm cultures 48h are put into；
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring, load
200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, and then 121 DEG C of min of high pressure moist heat sterilization 20 again, connect after cooling by 1%
Plant in amount access plastic bottle, shake uniform up and down, be placed on 28 DEG C of temperature, humidity 80% cultivates 7 d；The fermented bacterium of plastic bottle is pressed
Mass ratio：1：1000 are added in sterilized water, are stirred into making spore suspension, and the spore suspension is sent out as solid tray
Ferment seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterilized water（50：25：1：50）（Mass ratio）
After stirring, it is sub-packed in polybag, in stand-by after 30 min that sterilized at 121 DEG C；Seedling-cultivation plate is arranged at solid fermentation workshop
In, to solid fermentation workshop formaldehyde and potassium permanganate mixed liquor（2:1）（Mass ratio）The closed h of suffocating sterilization 24, then by
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate, spread
To thickness 2cm, under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, 7d is cultivated, then pours into desinfection chamber and carries out air-drying tray hair
Ferment product, the shallow tray fermentation product after air-drying is put into low-temperature grinding instrument after crushing, crosses 200 mesh sieves, and collection obtains long shoot trichoderma
DQ2 conidia powders.
Step 4：Above-mentioned long shoot trichoderma DQ2 conidia powders, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright powder
（50：6：1：16：6：9）（Mass ratio）Mixing, is configured to long shoot trichoderma DQ2 wettable powders.
Long shoot trichoderma DQ2 wettable powders manufactured in the present embodiment are used for pouring root, for preventing and treating soil-borne diseases of vegetable, root
Maize ear rot, stem rot, gray mold.
Claims (3)
1. a kind of long shoot trichoderma DQ2 wettable powders, it is characterised in that：This long shoot trichoderma DQ2 wettable powders pass through following
Preparation method is prepared from：
Step one：By long shoot trichoderma DQ2 inoculations in PDA slant mediums, 28 DEG C of culture 72-96 h use sterile purified water
Lower conidium is washed, spore liquid is made, spore liquid miospore number is 106Individual/mL, then by its volume for pressing PD nutrient solutions
1% is linked into the PD nutrient solutions of sterilizing, is put into 28 DEG C, 120rpm cultures 36-48h；
Step 2：By wheat bran, diatomite, rice husk, water in mass ratio 2:1:1:4 ratio mixing, after stirring, loads
200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, and then 121 DEG C of min of high pressure moist heat sterilization 20 again, connect after cooling by 1%
Plant in amount access plastic bottle, shake uniform up and down, be placed on 28 DEG C of temperature, the culture 5-7 of humidity 80% d；By the fermented bacterium of plastic bottle
In mass ratio：1：500-1：1000 add in sterilized water, are stirred into making spore suspension, and the spore suspension is as solid
Body shallow tray fermentation seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin, sterilized water in mass ratio 50：25：1：50 stirrings
After uniform, it is sub-packed in polybag, in stand-by after 20~30 min that sterilized at 121 DEG C；Seedling-cultivation plate is arranged at solid fermentation workshop
In, to solid fermentation workshop formaldehyde and the closed h of suffocating sterilization 24 of potassium permanganate mixed liquor, then by sterilization fermentation matrix
With shallow tray fermentation seed liquor in mass ratio 10：1 ratio mixing, is uniformly sprawled in seedling-cultivation plate, the paving to -2cm of thickness 1,
Under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, 7d is cultivated, then pours into the air-dried shallow tray fermentation product of progress in desinfection chamber, is air-dried
Shallow tray fermentation product afterwards is put into low-temperature grinding instrument after crushing, crosses 200 mesh sieves, and collection obtains long shoot trichoderma DQ2 conidia powders；
Step 4：Above-mentioned long shoot trichoderma DQ2 conidia powders, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright powder press matter
Amount compares 50：6：1：16：6：9 mixing, are configured to long shoot trichoderma DQ2 wettable powders.
2. a kind of application of the long shoot trichoderma DQ2 wettable powders described in claim 1, it is characterised in that：By long shoot wood
Mould DQ2 wettable powders are directly used in foliage-spray preventing and treating muskmelon, tomato powdery mildew, downy mildew, gray mold.
3. a kind of application of the long shoot trichoderma DQ2 wettable powders described in claim 1, it is characterised in that：By described long shoot
Trichoderma DQ2 wettable powders are used for pouring root, and for preventing and treating soil-borne diseases of vegetable, soil-borne diseases of vegetable includes root rot, stem rot, ash
Mildew.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710424515.8A CN107006524A (en) | 2017-06-07 | 2017-06-07 | A kind of long shoot trichoderma DQ2 wettable powders are prepared and applied |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710424515.8A CN107006524A (en) | 2017-06-07 | 2017-06-07 | A kind of long shoot trichoderma DQ2 wettable powders are prepared and applied |
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| CN107006524A true CN107006524A (en) | 2017-08-04 |
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| CN201710424515.8A Pending CN107006524A (en) | 2017-06-07 | 2017-06-07 | A kind of long shoot trichoderma DQ2 wettable powders are prepared and applied |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184907A (en) * | 2018-01-20 | 2018-06-22 | 海南大学 | A kind of wettable powder of trichoderma asperellum DQ1 compositions and application |
| CN113249228A (en) * | 2021-05-14 | 2021-08-13 | 河北科技大学 | Application of trichoderma longibrachiatum JG9-31 in prevention and treatment of gray mold |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212485A (en) * | 2011-05-09 | 2011-10-12 | 中国农业科学院农业资源与农业区划研究所 | Trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases |
| CN102388922A (en) * | 2011-10-21 | 2012-03-28 | 上海交通大学 | Preparation method and application of wettable powder for preventing and controlling Sphaerotheca fuliginea trichoderma of greenhouse cucumbers |
-
2017
- 2017-06-07 CN CN201710424515.8A patent/CN107006524A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212485A (en) * | 2011-05-09 | 2011-10-12 | 中国农业科学院农业资源与农业区划研究所 | Trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases |
| CN102388922A (en) * | 2011-10-21 | 2012-03-28 | 上海交通大学 | Preparation method and application of wettable powder for preventing and controlling Sphaerotheca fuliginea trichoderma of greenhouse cucumbers |
Non-Patent Citations (3)
| Title |
|---|
| 易征璇: "康氏木霉固体发酵产孢子粉工艺研究", 《现代农业科技》 * |
| 袁会珠: "《现代农药应用技术图解》", 30 June 2013, 北京：中国农业科学技术出版社 * |
| 陈捷: "《玉米病害诊断与防治》", 30 June 2001, 北京：金盾出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184907A (en) * | 2018-01-20 | 2018-06-22 | 海南大学 | A kind of wettable powder of trichoderma asperellum DQ1 compositions and application |
| CN113249228A (en) * | 2021-05-14 | 2021-08-13 | 河北科技大学 | Application of trichoderma longibrachiatum JG9-31 in prevention and treatment of gray mold |
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