CN108184907A - A kind of wettable powder of trichoderma asperellum DQ1 compositions and application - Google Patents
A kind of wettable powder of trichoderma asperellum DQ1 compositions and application Download PDFInfo
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- CN108184907A CN108184907A CN201810062911.5A CN201810062911A CN108184907A CN 108184907 A CN108184907 A CN 108184907A CN 201810062911 A CN201810062911 A CN 201810062911A CN 108184907 A CN108184907 A CN 108184907A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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Abstract
Wettable powder and application the present invention relates to a kind of trichoderma asperellum DQ1 compositions, the wettable powder of trichoderma asperellum DQ1 compositions therein are prepared by the following method：One：Spore liquid is made in trichoderma asperellum DQ1 bacterial strains, is cultivated；Two：Spore suspension is made, the spore suspension is as shallow tray fermentation seed liquor；Three：Sterilization fermentation matrix and shallow tray fermentation seed liquor are mixed, culture is air-dried, crushed, and obtains trichoderma asperellum DQ1 conidia powders；Four：By trichoderma asperellum DQ1 conidia powders, the jinggangmeisu that original powder content is 60%, 5000UI/ milligrams of Dipels in mass ratio 10：0.5‑1：0.5 1 mixing, are configured to trichoderma asperellum DQ1 compositions；Five：Wettable powder is made in trichoderma asperellum DQ1.The present invention can effectively play the drug effect of biological pesticide, while inhibit and kill the pest and disease damages such as sheath blight fungus and ustilaginoidea virens, nontoxic to people and animals, free from environmental pollution.
Description
First, technical field
The present invention relates to rice pest prevention biological pesticide, and in particular to a kind of trichoderma asperellum DQ1 compositions can
Wet powder and application.
2nd, background technology
In recent years, rice pest species survey in China's is more, and the degree that causes harm weight, especially rice sheath blight disease, false smut, rice indulge volume
Leaf snout moth's larva and snout moth's larva, which are laid particular stress on, to be occurred, serious threat China Rice Production safety.However relying primarily on of rice pest is prevented at present
Learn to do section, it is well known that chemical pesticide has residual in crop, empty gas and water and soil, causes pesticide residue exceeded and environment is dirty
Dye, and the use of single medicament easily causes disease pest and develops immunity to drugs, while also murders some beneficial microbes in soil, subtracts
Soil beneficial microbe group is lacked, has reduced soil organic matter content, reduce fertility and led to soil hardening, this has been brought all
More society, economy and ecological problems.With the improvement of people ' s living standards with the enhancing of environmental consciousness, rice pest is established
Green prevention and control implements rice pest green prevention and control measure, is the important measure for implementing " environment protective plant protecting " theory, is to ensure that rice
The important channel of production safety and paddy quality safety.
Trichoderma asperellum belongs to Fungi Imperfecti door, hyphomycetales, trichoderma.It is that a kind of distribution is wide, breeding is fast, has higher biological and ecological methods to prevent plant disease, pests, and erosion valency
Value and to the insensitive biological and ecological methods to prevent plant disease, pests, and erosion of some broad-spectrum germicides beneficial to fungi, the characteristics of adaptable strong, has a broad antifungal spectrum, moreover it is possible to promote
Into plant growth, there is hyperparasitism, Competition, generate lyases, generate antagonist compound, inhibit or kill cause of disease
The effects that bacterium, induction host plant are to the defense response of germ mode, has important economic implications.
3rd, invention content
It is an object of the present invention to provide a kind of wettable powder of trichoderma asperellum DQ1 compositions, this trichoderma asperellum DQ1
The wettable powder of composition solve the chemical pesticide used at present generated when preventing rice pest it is toxic to people and animals,
Environment is polluted, has the problem of residual, it is a further object to provide the wettable powders of this trichoderma asperellum DQ1 compositions
The application of agent.
To achieve these goals, the specific technical solution that the present invention uses is as follows：This trichoderma asperellum DQ1 compositions
Wettable powder is prepared by the following method：
Step 1：By trichoderma asperellum DQ1 inoculations in PDA slant mediums, 28 DEG C of culture 72-96 h use sterile purified water
Lower conidium is washed, spore liquid is made, spore liquid miospore number is 106A/mL, then by its volume by PD culture solutions
In the PD culture solutions of 1% access sterilizing, 28 DEG C are put into, 120rpm culture 36-48h obtain first order seed culture solution；
Step 2：By wheat bran, diatomite, rice husk, water in mass ratio 2:1:1:4 ratio mixing, after stirring evenly, is packed into
200mL plastic bottles, charge weight are the 1/2 of plastics bottle height, and then 121 DEG C of 20 min of high pressure moist heat sterilization again, connect after cooling by 1%
Kind amount accesses first order seed culture solution in plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, and humidity 80% cultivates 5-7d；It will
The fermenting microbe of plastic bottle is in mass ratio：1：500-1：1000 add in sterile water, are stirred into making spore suspension, should
Spore suspension is as shallow tray fermentation seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin, sterile water in mass ratio 50：25：1：50 stirrings
After uniformly, it is sub-packed in polybag, it is for use after 20~30 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation workshop
In, to solid fermentation workshop formaldehyde and the closed suffocating sterilization of potassium permanganate mixed liquor for 24 hours, then by sterilization fermentation matrix and
Shallow tray fermentation seed liquor in mass ratio 10：1 ratio mixing, uniformly sprawls in seedling-cultivation plate, spreads to thickness 1-2cm, in perseverance
It is 28 DEG C warm, under conditions of moisturizing RH >=80%, 7d is cultivated, then pour into desinfection chamber and carry out air-drying shallow tray fermentation product, after air-drying
Shallow tray fermentation product be put into low-temperature grinding instrument crush after, cross 200 mesh sieve, collection obtain trichoderma asperellum DQ1 conidia powders；
Step 4：Jinggangmeisu, the 5000UI/ millis of trichoderma asperellum DQ1 conidia powders, original powder content prepared by step 3 for 60%
Gram Dipel in mass ratio 10：0.5-1：0.5-1 is mixed, and is configured to trichoderma asperellum DQ1 compositions；
Step 5：By above-mentioned trichoderma asperellum DQ1 compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, press without bright powder
Mass ratio 50：6：1：16：6：9 mixing are configured to the wettable powder of trichoderma asperellum DQ1 compositions.
The application of the wettable powder of above-mentioned trichoderma asperellum DQ1 compositions：By the trichoderma asperellum DQ1 compositions can
Wet powder is sprayed on rice, for any one pest and disease damage in water cnaphalocrocis, yellow rice borer, striped rice borer, banded sclerotial blight, false smut
Prevention.
Advantageous effect：
1st, the present invention can promote rice to absorb and grow fertilizer, improve rice products quality, and inducing paddy rice generates resistance, can
To inhibit Rhizoctonia solani Kuhn and ustilaginoidea virens growth and breeding.
2nd, the present invention can effectively play the drug effect of biological pesticide, can inhibit simultaneously and kill sheath blight fungus and false smut
The pest and disease damages such as bacterium, water cnaphalocrocis, yellow rice borer, striped rice borer, it is nontoxic to people and animals, it is free from environmental pollution.
4th, specific embodiment
The present invention is described further below：
Embodiment 1：
The wettable powder of this trichoderma asperellum DQ1 compositions is prepared by following preparation method：
Step 1：By trichoderma asperellum DQ1 inoculations in PDA slant mediums, 28 DEG C of culture 72h are washed down with aseptic distillation
Conidium, is made spore liquid, and spore liquid miospore number is 106A/mL, then by 1%（Volume ratio）The PD trainings of access sterilizing
In nutrient solution, 28 DEG C are put into, 120rpm culture 36h obtain first order seed culture solution.
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring evenly, dress
Enter 200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, then 121 DEG C of high pressure moist heat sterilization 20min again, is connect after cooling by 1%
Kind amount accesses first order seed culture solution in plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, and humidity 80% cultivates 6d；It will modeling
Expect the fermenting microbe of bottle in mass ratio：1：500 add in sterile water, are stirred into making spore suspension, the spore suspension
Liquid is as shallow tray fermentation seed liquor；It is known as material in the present invention after wheat bran, diatomite, rice husk, water mixing, 1% inoculum concentration is
The mass ratio of material in the volume and plastic bottle of the first order seed culture solution referred to the accession to.
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterile water（50：25：1：50）（Quality
Than）After stirring evenly, it is sub-packed in polybag, it is for use after 20 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation vehicle
Between in, to solid fermentation workshop formaldehyde and potassium permanganate（2:1）（Mass ratio）The closed suffocating sterilization of mixed liquor for 24 hours, then will
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate,
Paving under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, cultivates 7d, then pour into desinfection chamber and carry out air-drying tray to thickness 1cm
Tunning, the shallow tray fermentation product after air-drying are put into low-temperature grinding instrument after crushing, cross 200 mesh sieve, and collection obtains spine spore wood
The conidia powder of mould DQ1.
Step 4：Above-mentioned trichoderma asperellum DQ1 conidia powders, jinggangmeisu (original powder：60%), Dipel（Unit：
5000UI)（10：1：1）（Mass ratio）Mixing, is configured to trichoderma asperellum DQ1 compositions.
Step 5：By above-mentioned trichoderma asperellum DQ1 compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright
Powder（50：6：1：16：6：9）（Mass ratio）Mixing is configured to the wettable powder of trichoderma asperellum DQ1 compositions.
The wettable powder of the present embodiment trichoderma asperellum DQ1 compositions is directly applied into rice, rice banded sclerotial blight can be prevented
The pest and disease damages such as germ and ustilaginoidea virens, water cnaphalocrocis, yellow rice borer, striped rice borer.
Embodiment 2：
The wettable powder of this trichoderma asperellum DQ1 compositions is prepared by following preparation method：
Step 1：By trichoderma asperellum DQ1 inoculations in PDA slant mediums, 28 DEG C of 96 h of culture are washed down with aseptic distillation
Conidium, is made spore liquid, and spore liquid miospore number is 106A/mL, then by 1%（Volume ratio）The PD trainings of access sterilizing
In nutrient solution, 28 DEG C are put into, 120rpm cultures 40h.
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring evenly, dress
Enter 200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, then 121 DEG C of 20 min of high pressure moist heat sterilization again, and 1% is pressed after cooling
It in inoculum concentration access plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, humidity 80% cultivates 7d；By the fermenting microbe of plastic bottle
In mass ratio：1：800 add in sterile water, are stirred into making spore suspension, the spore suspension is as solid tray
Fermentation seed liquid.
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterile water（50：25：1：50）（Quality
Than）After stirring evenly, it is sub-packed in polybag, it is for use after 30 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation vehicle
Between in, to solid fermentation workshop formaldehyde and potassium permanganate mixed liquor（2:1）（Mass ratio）Closed suffocating sterilization for 24 hours, then will
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate,
Paving under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, cultivates 7d, then pour into desinfection chamber and carry out air-drying tray to thickness 2cm
Tunning, the shallow tray fermentation product after air-drying are put into low-temperature grinding instrument after crushing, cross 200 mesh sieve, and collection obtains spine spore wood
The conidia powder of mould DQ1.
Step 4：Above-mentioned trichoderma asperellum DQ1 conidia powders, jinggangmeisu (original powder：60%), Dipel（Unit：
5000UI)（10：0.5：1）（Mass ratio）Mixing, is configured to trichoderma asperellum DQ1 compositions.
Step 5：By above-mentioned trichoderma asperellum DQ1 compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright
Powder（50：6：1：16：6：9）（Mass ratio）Mixing is configured to the wettable powder of trichoderma asperellum DQ1 compositions.
The wettable powder of the present embodiment trichoderma asperellum DQ1 compositions is directly applied into rice, rice banded sclerotial blight can be prevented
The pest and disease damages such as germ and ustilaginoidea virens, water cnaphalocrocis, yellow rice borer, striped rice borer.
Embodiment 3：
The wettable powder of this trichoderma asperellum DQ1 compositions is prepared by following preparation method：
Step 1：By trichoderma asperellum DQ1 inoculations in PDA slant mediums, 28 DEG C of culture 84h are washed down with aseptic distillation
Conidium, is made spore liquid, and spore liquid miospore number is 106A/mL, then by 1%（Volume ratio）The PD trainings of access sterilizing
In nutrient solution, 28 DEG C are put into, 120rpm cultures 48h.
Step 2：Wheat bran, diatomite, rice husk, water are pressed 2:1:1:4（Mass ratio）Ratio mixing, after stirring evenly, dress
Enter 200mL plastic bottles, charge weight is the 1/2 of plastics bottle height, then 121 DEG C of 20 min of high pressure moist heat sterilization again, and 1% is pressed after cooling
It in inoculum concentration access plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, humidity 80% cultivates 7 d；By the fermenting microbe of plastic bottle
In mass ratio：1：1000 add in sterile water, are stirred into making spore suspension, the spore suspension is as solid tray
Fermentation seed liquid.
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin and sterile water（50：25：1：50）（Quality
Than）After stirring evenly, it is sub-packed in polybag, it is for use after 30 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation vehicle
Between in, to solid fermentation workshop formaldehyde and potassium permanganate mixed liquor（2:1）（Mass ratio）Closed suffocating sterilization for 24 hours, then will
Sterilization fermentation matrix and shallow tray fermentation seed liquor press 10：1（Mass ratio）Ratio mixing, uniformly sprawl in seedling-cultivation plate,
Paving under conditions of 28 DEG C of constant temperature, moisturizing RH >=80%, cultivates 7d, then pour into desinfection chamber and carry out air-drying tray to thickness 2cm
Tunning, the shallow tray fermentation product after air-drying are put into low-temperature grinding instrument after crushing, cross 200 mesh sieve, and collection obtains spine spore wood
The conidia powder of mould DQ1 compositions.
Step 4：Above-mentioned trichoderma asperellum DQ1 conidia powders, jinggangmeisu (original powder：60%), Dipel（Unit：
5000UI)（10：0.5：0.5）（Mass ratio）Mixing, is configured to trichoderma asperellum DQ1 compositions.
Step 5：By above-mentioned trichoderma asperellum DQ1 compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, without bright
Powder（50：6：1：16：6：9）（Mass ratio）Mixing is configured to the wettable powder of trichoderma asperellum DQ1 compositions.
The wettable powder of the present embodiment trichoderma asperellum DQ1 compositions is directly applied into rice, rice banded sclerotial blight can be prevented
The pest and disease damages such as germ and ustilaginoidea virens, water cnaphalocrocis, yellow rice borer, striped rice borer.
Claims (2)
1. a kind of wettable powder of trichoderma asperellum DQ1 compositions, it is characterised in that：This trichoderma asperellum DQ1 compositions can
Wet powder is prepared by the following method：
Step 1：By trichoderma asperellum DQ1 inoculations in PDA slant mediums, 28 DEG C of culture 72-96 h use sterile purified water
Lower conidium is washed, spore liquid is made, spore liquid miospore number is 106A/mL, then by its volume by PD culture solutions
In the PD culture solutions of 1% access sterilizing, 28 DEG C are put into, 120rpm culture 36-48h obtain first order seed culture solution；
Step 2：By wheat bran, diatomite, rice husk, water in mass ratio 2:1:1:4 ratio mixing, after stirring evenly, is packed into
200mL plastic bottles, charge weight are the 1/2 of plastics bottle height, and then 121 DEG C of 20 min of high pressure moist heat sterilization again, connect after cooling by 1%
Kind amount accesses first order seed culture solution in plastic bottle, shakes up and down uniformly, is placed on 28 DEG C of temperature, and humidity 80% cultivates 5-7d；It will
The fermenting microbe of plastic bottle is in mass ratio：1：500-1：1000 add in sterile water, are stirred into making spore suspension, should
Spore suspension is as shallow tray fermentation seed liquor；
Step 3：Shallow tray fermentation matrix is with wheat bran, diatomite, streptomysin, sterile water in mass ratio 50：25：1：50 stirrings
After uniformly, it is sub-packed in polybag, it is for use after 20~30 min that sterilize at 121 DEG C；Seedling-cultivation plate is set to solid fermentation workshop
In, to solid fermentation workshop formaldehyde and the closed suffocating sterilization of potassium permanganate mixed liquor for 24 hours, then by sterilization fermentation matrix and
Shallow tray fermentation seed liquor in mass ratio 10：1 ratio mixing, uniformly sprawls in seedling-cultivation plate, spreads to thickness 1-2cm, in perseverance
It is 28 DEG C warm, under conditions of moisturizing RH >=80%, 7d is cultivated, then pour into desinfection chamber and carry out air-drying shallow tray fermentation product, after air-drying
Shallow tray fermentation product be put into low-temperature grinding instrument crush after, cross 200 mesh sieve, collection obtain trichoderma asperellum DQ1 conidia powders；
Step 4：Jinggangmeisu, the 5000UI/ millis of trichoderma asperellum DQ1 conidia powders, original powder content prepared by step 3 for 60%
Gram Dipel in mass ratio 10：0.5-1：0.5-1 is mixed, and is configured to trichoderma asperellum DQ1 compositions；
Step 5：By above-mentioned trichoderma asperellum DQ1 compositions, ProDisMC9, WgwinDKF, LT-8601, white carbon, press without bright powder
Mass ratio 50：6：1：16：6：9 mixing are configured to the wettable powder of trichoderma asperellum DQ1 compositions.
2. a kind of application of the wettable powder of trichoderma asperellum DQ1 compositions described in claim 1, it is characterised in that：By institute
The wettable powder of trichoderma asperellum DQ1 compositions stated is sprayed on rice, withered for water cnaphalocrocis, yellow rice borer, striped rice borer, line
Any one prevention and control of plant diseases, pest control in disease, false smut.
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| CN201810062911.5A CN108184907A (en) | 2018-01-20 | 2018-01-20 | A kind of wettable powder of trichoderma asperellum DQ1 compositions and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109112071A (en) * | 2018-08-16 | 2019-01-01 | 浙江农林大学 | A kind of Trichoderma and its application |
| CN109517744A (en) * | 2019-01-10 | 2019-03-26 | 海南大学 | The composite spore powder and its application of Trichoderma mixed cooperative solid fermentation preparation |
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| CN107006524A (en) * | 2017-06-07 | 2017-08-04 | 刘铜 | A kind of long shoot trichoderma DQ2 wettable powders are prepared and applied |
| CN107114411A (en) * | 2017-06-07 | 2017-09-01 | 刘铜 | For the trichoderma asperellum DQ3 compound powders YBY of raising rice seedlings and application |
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- 2018-01-20 CN CN201810062911.5A patent/CN108184907A/en active Pending
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| CN107006524A (en) * | 2017-06-07 | 2017-08-04 | 刘铜 | A kind of long shoot trichoderma DQ2 wettable powders are prepared and applied |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109112071A (en) * | 2018-08-16 | 2019-01-01 | 浙江农林大学 | A kind of Trichoderma and its application |
| CN109517744A (en) * | 2019-01-10 | 2019-03-26 | 海南大学 | The composite spore powder and its application of Trichoderma mixed cooperative solid fermentation preparation |
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